LOCUS       NZ_WHOM01000061        11506 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 504, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000061
VERSION     NZ_WHOM01000061.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 229883)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 229883)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 64869
            Orig. end                         :: 76375
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11506
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11506
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11506
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6506](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6506
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            243..1076
                     /locus_tag="GCN81_RS16855"
     CDS             243..1076
                     /GO_function="GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063346.1"
                     /locus_tag="GCN81_RS16855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="WP_156725584.1"
                     /transl_table=11
                     /translation="MRALLRVPRGKRLDLASHDARATPGGPPSKAAGLAATAALAEPLA
                     DLQERLYAASTAGDRRRVLLVLQGMDTSGKGGTVKHVIGLFNPSGCRIRAFKTPTPEER
                     NHPFLWRVMRALPQPGEIGIFDRSHYEDVLITRVRELVPHRHLGRRYGQINRFEKSLAL
                     DGVTLVKCFLHISYEEQRARLLKRLDRPDKHWKFNPSDIDDRALWPAYQEAYAIALERC
                     STGTAPWYLIPSDRKWYRNWAISTLLLAHLEALDPQYPTPDFDLADARERLLTNT"
     misc_feature    complement(1089..1098)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            1137..1610
                     /locus_tag="GCN81_RS16860"
     CDS             1137..1610
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093485.1"
                     /locus_tag="GCN81_RS16860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="OsmC family protein"
                     /protein_id="WP_156725456.1"
                     /transl_table=11
                     /translation="MTDTHTYDVEIEWTGNLGSGTDSYRSFSRDHEVLATGKAPIAASS
                     DPAFRGDPTRWNPEELLVASVAQCHMLWYLHLCTVGGVTVVGYEDRAHGVMTMDASGGG
                     GQLTEVVLRPEVTVADASMVDKARALHGEVHAVCFIARSVNFPIRPEPVIRVQ"
     gene            1715..2611
                     /locus_tag="GCN81_RS16865"
     CDS             1715..2611
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034067.1"
                     /locus_tag="GCN81_RS16865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_156725457.1"
                     /transl_table=11
                     /translation="MILSVRRLAACCALGTALAGCGGPGLPHTTGAESPTAPPAASPAA
                     PAATADAQPPPPTLAPGPEGQAPVFEHGPRGGGKTVALTFDTDMTADQGPRAASGERFD
                     NPELIGTLRRLKVPSTVFMTGRWAEEYPDQARSIGADPLFEVANHSYSHHAYTARCYGL
                     PTLAPQRMRADVDRAFAAFRAAGVARTVPYFRFPGGCYDRTALRAIAGSGVTAVQWDVV
                     GGDAFATDPDAVAREVLRGVRPGSVVVLHCTRSAAPATERALRTVVPELRERGYRFVRV
                     SELIRASDQGGRGQGAG"
     gene            2633..2833
                     /locus_tag="GCN81_RS16870"
     CDS             2633..2833
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981773.1"
                     /locus_tag="GCN81_RS16870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156725458.1"
                     /transl_table=11
                     /translation="MDPTDHRARTSLGTPGPDEATPPADGPPFAECVLCRNPTEYPEST
                     KGATLCPVCAWQEAQRSACSG"
     gene            2944..3534
                     /locus_tag="GCN81_RS16875"
     CDS             2944..3534
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1224: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892352.1"
                     /locus_tag="GCN81_RS16875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_156725459.1"
                     /transl_table=11
                     /translation="MVTAKTTPRGRPRDARAHAAILTTAAELVTETGYAAMSLGAVAAR
                     ARVGKDTIYRRWSGKAELVYEAVFTTTDTAPVPDTGTLEGDLTVLVQQLIDEFSAPAAA
                     AALPGLLADFAAAPELRATIRSAFLAPAKQRLADVFDRARARGETVPGVESDLVLDSLV
                     GTVLFRLGILGEPASPRLARSVAGIAAKGIEAR"
     gene            3531..3905
                     /locus_tag="GCN81_RS16880"
     CDS             3531..3905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949070.1"
                     /locus_tag="GCN81_RS16880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156725460.1"
                     /transl_table=11
                     /translation="MNGWLLAAGALATVTALIHLIAGGREVVRPLLGSSLEAEPLRILH
                     AVWHFATADLLFSGAALIALAVRPAPNVPLVLFIAAHFLAYAAVFLVITLRAGWSRPLL
                     RLPQWMMLLPVAALAGAGVL"
     gene            4079..4849
                     /locus_tag="GCN81_RS16885"
     CDS             4079..4849
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944754.1"
                     /locus_tag="GCN81_RS16885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_197287683.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLAALAYPVWSYADRSGTGEANLEASTVA
                     TQWGPLSATDRDLLVKVRLAGLWEIPAGQQAMERAPTEAIKEAGDHLVVGHTDLDRRVR
                     QVAAQLGVELPSQPTEQQQGWLRELTEAQGEVYERKFANILRDSHGKVFSLIASVRHTT
                     RNALVRQLATDSNQTVLDHITMLERTGDVDFDALARDAAGRSTASPSGPPPPVPGNTPL
                     PPPAAGPTGDDQSSTSRPSAPGTADINTNRPEVG"
     gene            5001..6506
                     /locus_tag="GCN81_RS16890"
     CDS             5001..6506
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944755.1"
                     /locus_tag="GCN81_RS16890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_156725462.1"
                     /sec_met_domain="DUF692 (E-value: 1.1e-95, bitscore: 313.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGRVRLGTGIGWRPEIAEAVERMPGVDWVEVVAENVCPGHIAPP
                     LLRLRERGVNVVPHGVGLGLGGAERPDPARLAALAERAEALGSPLVSEHIAFVRAGGAL
                     TASQRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALIGWPGEEMTEGR
                     FLSELVERTGVRLLIDVANLYTNQVNRGEDASAALDELPVEAIAYVHVAGGATRDGVWH
                     DSHGHPVPQPVLDILADLASRVTPPGVLLERDENFPAQAELERELDTLRAAVSRDRLLA
                     TAGPSAGVPAGRRPGVTSKRPSGDTTRRPPSTVTTGRPSGAPLALATGPAWRPVQGTAP
                     AAVPATAATRDVPDAPDAPAQASDAARQRVALRQASLLSALVAGTPPPEGFDGQRLRVQ
                     SRALTAKRASVVAKVAPELPEILGAEYRSAFLAYARNRPLTAGHRRDALAFAEHLLRAR
                     KPRSPRARRELVRWWLERSGPAPLTGRPLARLARTARYALLRR"
     gene            6640..8283
                     /locus_tag="GCN81_RS16895"
     CDS             6640..8283
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (111-488). E-value:
                     1.9e-24. Score: 78.6. Matches aSDomain:
                     nrpspksdomains_GCN81_RS16895_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133766.1"
                     /locus_tag="GCN81_RS16895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_156725463.1"
                     /sec_met_domain="PF00561 (E-value: 2.1e-29, bitscore: 96.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKHGKRGRAPLLYAVTGSLVLTGLAASPASPAASASSTPEAPEA
                     RGTRVAVARAEAAGISFGRCPEVERLPAAVQCGTVTVPLDYADPDGKQIKLTVSRVKAK
                     DPAKRQGALIFNPGGPGGSGMYFPVVSTAGLPAFRRIAREYDVVGYAPRGVGRSAPVSC
                     KDPATFDPAPTRSPRHPDAAYKQERVARAKTYARGCLNRTGEALRHYHSLNNARDLDVL
                     RAGLGERKLNFLGASYGTYFGALYAAMFPSHVRRMVFDSAVNPDPSQIWYQNNLDQSLA
                     FERRWADFRAWVAAHHTTYGLGRTPQQVAHSYEKVRAALAREPAGGKVGTGELQAAFLQ
                     TGYHDDVWPKRAAALTAYLNGDPKPLVQQAARSSERAAENENGNAVYTAVECNDAPWPT
                     DFETWDEDNSRLARVAPFETWDNAWMNLPCAYWPVPRQRPLDLRIPDGALPPTLILAAE
                     RDAATPYQGAWELHRRLPDSALVTERKAGTHGLVGGPNQCVNGHLEAYLLDGRLPARRA
                     AGCAPRPEPKPEGEPEAAGSREATRTPRTA"
     aSDomain        6973..8103
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS16895_Abhydrolase_1.1"
                     /evalue="1.90E-24"
                     /label="GCN81_RS16895_Abhydrolase_1.1"
                     /locus_tag="GCN81_RS16895"
                     /protein_end="488"
                     /protein_start="111"
                     /score="78.6"
                     /tool="antismash"
                     /translation="ALIFNPGGPGGSGMYFPVVSTAGLPAFRRIAREYDVVGYAPRGVG
                     RSAPVSCKDPATFDPAPTRSPRHPDAAYKQERVARAKTYARGCLNRTGEALRHYHSLNN
                     ARDLDVLRAGLGERKLNFLGASYGTYFGALYAAMFPSHVRRMVFDSAVNPDPSQIWYQN
                     NLDQSLAFERRWADFRAWVAAHHTTYGLGRTPQQVAHSYEKVRAALAREPAGGKVGTGE
                     LQAAFLQTGYHDDVWPKRAAALTAYLNGDPKPLVQQAARSSERAAENENGNAVYTAVEC
                     NDAPWPTDFETWDEDNSRLARVAPFETWDNAWMNLPCAYWPVPRQRPLDLRIPDGALPP
                     TLILAAERDAATPYQGAWELHRRLPDSALVTERKAGT"
     gene            complement(8325..9044)
                     /gene="hemQ"
                     /locus_tag="GCN81_RS16900"
     CDS             complement(8325..9044)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643864.1"
                     /locus_tag="GCN81_RS16900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_156725464.1"
                     /transl_table=11
                     /translation="MSDDAPAKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRTGYA
                     DEVQELFDQLAAKDITVRGTYDVSGLRADADLMIWWHAETADELQEAYNLFRRTKLGRA
                     LEPVWSNMALHRPAEFNKSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRRMLAD
                     HGKMARGYPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRASEARMHVREEVP
                     FYTGRRKSVADLVAGLA"
     gene            complement(9049..10509)
                     /gene="hemG"
                     /locus_tag="GCN81_RS16905"
     CDS             complement(9049..10509)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993744.1"
                     /locus_tag="GCN81_RS16905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_156725465.1"
                     /sec_met_domain="DAO (E-value: 2e-13, bitscore: 44.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKTDTRTSHVVVIGGGIAGLAAAHRLLGTGARVTVLEAADRLGG
                     KLHAGEIEGVRVDLGAESMLARRPEAVELARAVGLGDLLQPPATATAALWTRGELRPMP
                     KGHVMGVPGDAGALAGVLSDEGLRRIEQDRELPPTEVGDDIAVGEYVASRVGREVVDRL
                     VEPLLGGVYAGDAYRLSMRMAVPKLYEAARTHRSLLDGVRDIQRQAVEAGATGPVFMGI
                     AGGVGGLPLAVADALRARGAEIRLNCPVRGVRRVADGWEVDIENGEDGGIKADAVVIAV
                     PAPQAARLLDADVPAAAAELRAVEYASMALMTLAYRRTDANLPAGSGFLVPPVDGHTIK
                     ASTFASQKWGWIADEDPDVFVLRTSVGRYGEERDLRRDDAELIDVSRRDLREATGLTAT
                     PVATRVTRWNDGLPQYPVGHHARVARIREHVAKLPGLAVCGAAYDGVGIPACIASAYAA
                     VDQLGGDSSALDQLTGNPVQSLHGGAAE"
ORIGIN
        1 ggccccagag gtcgcggccc tcgtcattcc ggcgcaggat cgaggggact gacccaaagg
       61 ggagcgcggt ggccaagaag aacgcgaggg acgacaacaa cgctggcaca ggcgcgcgcg
      121 acgcgggcgc gaaaggcacg ggcgcgggcg cgggcgcgaa aggcgccggt cagcgcgcca
      181 gccagggcgc ggaccagcgg cgtacgggca ggaagagtga cggcccggcc aaccccgccg
      241 acctgcgcgc gctgctccgc gtcccccggg ggaagcgcct cgacctcgcg tcccacgacg
      301 ccagggcgac acccggcggc ccgcccagca aggccgcggg cctggcggcg accgccgcac
      361 tggccgaacc actcgccgac ctccaggagc ggctgtacgc ggcctccacg gcgggcgacc
      421 ggcgccgggt cctgctggtg ctccaaggca tggacaccag cggcaagggc ggcacggtca
      481 agcacgtcat cggcctcttc aacccgtccg gctgccggat cagggccttc aagaccccca
      541 caccggaaga gcgaaaccac ccgttcctgt ggcgcgtcat gcgggccctg ccccagccgg
      601 gcgagatcgg catcttcgac cgctcgcact acgaggacgt actgatcacc cgggtcaggg
      661 agttggtgcc gcaccgccat ctgggtcgcc gctacgggca gatcaaccgc ttcgagaagt
      721 ccctggcact cgacggcgtg acgctcgtga agtgtttcct gcacatcagc tacgaggaac
      781 agcgggcccg gctcctgaag cggctcgacc gccccgacaa acactggaag ttcaacccca
      841 gcgacatcga cgaccgcgcg ctgtggcccg cctaccagga ggcctacgcg atcgccctgg
      901 aacgctgctc cacgggcacc gcgccctggt acctcatccc gtccgaccgc aagtggtacc
      961 ggaactgggc gatcagcacc ctgctgctcg cccacctcga agcgctggac ccgcagtacc
     1021 ccacgccgga cttcgacctc gcggacgccc gcgagcggct cctcacgaac acctgacccc
     1081 gcacagcctc gaaaatcacc tgccgctgaa gggatgaccc tccctatcct ggccgcatga
     1141 cggacaccca cacgtatgac gtagagatcg agtggaccgg caacctcggt tcaggcacgg
     1201 acagttaccg ttccttcagc cgtgaccacg aagtcctggc gacgggcaag gcgcccatcg
     1261 ccgcctcctc cgatccggcc ttccgcggcg accccacccg ctggaatccc gaggaactgc
     1321 tcgtggcgtc cgtggctcag tgccacatgc tctggtacct ccacctgtgc accgtgggag
     1381 gggtgacggt cgtcggatac gaagaccggg cgcacggcgt catgaccatg gacgcgagcg
     1441 gtggaggcgg ccagctgacc gaggtcgtcc tgcgtcccga ggtgaccgtg gccgacgcct
     1501 cgatggtgga caaggcccgg gcgctgcacg gcgaggtcca tgccgtgtgc tttatcgccc
     1561 gctcggtgaa cttccccatc cgccctgagc ccgtgatccg cgtccagtag ggccgggccg
     1621 gtgagcgtgc taggtgggtg acttcggcga cgggcacgcc gggtcgatcg cccagttctc
     1681 cgattagtgg cttttcttcc gttacgttca cctggtgatc ctctctgtac ggagactcgc
     1741 cgcctgctgt gcgctgggga cggcgctggc cgggtgcggc ggccccggcc tcccgcacac
     1801 caccggcgcg gaatccccca cagcgccacc tgccgcatcc cccgcggccc ccgcggccac
     1861 cgctgacgcc cagccgccac cccccacgct cgccccgggc cccgaaggcc aggcgcccgt
     1921 gttcgagcac ggcccgcgcg gcggcggcaa gaccgtagcg ctcacctttg acaccgacat
     1981 gaccgccgac cagggcccgc gcgcggcatc cggcgagcgc ttcgacaacc ccgagctgat
     2041 cgggaccctg cgccgcctca aggtcccctc caccgtcttc atgaccggcc gctgggccga
     2101 ggagtacccc gaccaggcca ggtcgatcgg cgccgacccg ctcttcgagg tcgccaacca
     2161 ctcctacagc caccacgcct acacagcgcg ctgctacggc ctgccgaccc tcgctcccca
     2221 gcggatgcga gccgacgtgg accgcgcctt cgccgccttc cgcgcggcgg gcgtggcgcg
     2281 caccgtgccg tacttccgct tccccggcgg ctgctacgac cgcacggcgc tgcgcgcgat
     2341 cgccgggagc ggggtcaccg cggtgcagtg ggacgtggtg ggcggcgacg ccttcgcgac
     2401 ggacccggac gcggttgccc gcgaggtact gcgcggggtg cggccgggct cggtggtcgt
     2461 gctgcactgc acccgcagcg cggccccggc caccgagcgc gcgctccgta cggtggtgcc
     2521 ggaactccgc gagcgcggat accggttcgt ccgggtctcc gagctgatca gggcatcgga
     2581 ccagggcggg cgcggccaag gcgccggtta gggcggcggc tacgctggcg gtatggaccc
     2641 cacggaccac cgcgcgcgta cgtcactggg gacgcccggc cccgacgagg ccacaccgcc
     2701 cgccgacggg cccccgttcg cggagtgcgt gctgtgccgg aacccgaccg agtacccgga
     2761 gtcgacgaag ggggcgacgc tgtgccccgt ctgcgcatgg caagaggctc agcgctccgc
     2821 ctgctccggc tgaacccggg ctagccaggc cagctgagca gccaccccaa gcgcagaacg
     2881 gcttcctcgg tgaagtcagc gtggacgata ttccatacgg gacgacccgt atggtaaaca
     2941 aggatggtga ctgccaagac aactccgcgc ggacgcccgc gagatgcgcg tgcgcatgcg
     3001 gcgatcctca ccaccgccgc cgagctggtg actgaaactg gctatgccgc catgtccctc
     3061 ggggccgtgg cagcccgggc cagggtgggc aaggacacca tctaccgccg ctggtccggc
     3121 aaggccgagc tggtctacga ggcggtgttc accaccaccg acaccgcccc cgtgcccgac
     3181 accggcaccc tcgaaggcga cctcaccgtc ctcgtccagc agctgatcga cgagttcagc
     3241 gcaccggccg ccgcggcggc gctgcccggc ctgctggcgg acttcgccgc cgcccccgaa
     3301 ctgcgcgcca ccatccgctc cgccttcctc gcgcccgcca agcagcgact ggccgatgtc
     3361 ttcgaccggg cgcgggcgcg gggcgagacc gtcccgggtg tggagtccga cctcgtgctc
     3421 gactccctcg tcggcaccgt cctgttccgg ctgggcatcc tcggcgagcc cgccagcccc
     3481 cgactcgccc gaagcgtcgc cgggatcgcc gccaagggaa tcgaggcccg gtgaacggat
     3541 ggctgctggc cgcgggagcc ctggcaaccg tgacggccct gatccacctc atcgccggcg
     3601 ggcgcgaggt ggtccgccca ctgctgggca gctccctgga ggccgagccg ttgcgcatcc
     3661 tgcacgcggt gtggcacttc gccacggcgg acctcctgtt cagcggcgcc gcgttgatcg
     3721 ccctcgcggt ccggcccgcg ccgaatgtgc cgctcgtcct cttcatcgcg gcccacttcc
     3781 tcgcgtatgc cgcggtgttc ttggtgatca cgctccgcgc gggctggtca cggcccctgc
     3841 tgcggctgcc gcagtggatg atgctgcttc cggtggcggc cctggccgga gccggggtgc
     3901 tgtagcgaca ccggttccgt cggctgccgg tacgcccgtg agcggaatct cagcccattg
     3961 cgggctgaac acgccctctt cgcatcgcgt atctctcaat accgtcaatc ggcttgaaca
     4021 ccagaccggt tgccggttgg ttcgtgcgaa gaacgcgcta cccccaggag ttaccttgtt
     4081 gcgacgcatc aacggcacgg ccctcatcat cgcggcgctg gtggcgacac tcgccgcgct
     4141 cgcctacccc gtctggtcgt acgccgaccg ctcgggcacc ggcgaggcca acctggaggc
     4201 cagtaccgtc gccacccagt gggggccgct cagcgcgacc gaccgggatc tgctggtgaa
     4261 agtcaggctt gccggactgt gggagatccc cgcgggtcag caggccatgg agcgggctcc
     4321 gacggaggcc atcaaggaag cgggcgacca cctggtggtc gggcacaccg acctggaccg
     4381 gcgggtacga caggtggccg cgcagctcgg ggtcgagctg ccgagccagc cgaccgagca
     4441 gcagcagggc tggctgcggg agctgacgga agcccaaggc gaggtctacg agcggaagtt
     4501 cgcgaacatt ctgcgcgact cacacggcaa ggtcttctcg ctgatcgcga gcgtgcggca
     4561 caccacccgc aacgcgctcg tacggcaact ggcgaccgac tcgaaccaga cggtactcga
     4621 ccacatcacg atgctggagc ggaccggcga cgtggacttc gacgcgctgg ccagggacgc
     4681 cgcgggcagg tcgaccgcca gcccgagcgg accgccgccg cccgtcccgg gcaacacccc
     4741 gctgccgccg cccgcggccg ggccgaccgg cgacgaccag tcctccacct cgcgcccctc
     4801 ggcgcccggc acggcggaca tcaacaccaa ccgccctgag gtgggctgac acgcttcacg
     4861 tccacggctg aaaaccggcg gtgaaacctc aaatcgagct ctgaatgtcc ccctgggtgg
     4921 gttcagcggc ccagtccggg ccagaagaac ctcacgccgt gcaggagggg gaaaccatga
     4981 gcggtggtat gagagatggc atgagcggcc gagtgcggct gggtacgggg atcggctggc
     5041 gcccggagat cgccgaagcg gtggagcgga tgcccggggt ggactgggtc gaggtggtcg
     5101 cggagaacgt ctgtccgggg catatcgccc caccgctgct acggctgcgc gagcgcggcg
     5161 tgaacgtggt gccgcatggc gtcggtcttg gcctgggtgg tgcggagcgg ccggacccgg
     5221 cgcggctcgc cgcgctcgcc gagcgggccg aggcgctggg ctcgccgctg gtcagcgagc
     5281 acatcgcgtt cgtacgggcg ggtggggcgc tgaccgcgtc gcagcggctg gaggccggac
     5341 acctgctgcc ggtgccgcgc accagggacg cgctggacgt gctgtgcgag aacgtacgga
     5401 tcgcgcagga cgcgctgccg gtgccgctcg ccctggagaa catcgccgcg ctgatcggct
     5461 ggccgggtga ggagatgacc gaggggcggt tcctgtccga actggtcgag cgcacgggcg
     5521 tacggctcct catcgacgtc gccaacctgt acaccaatca ggtcaaccgg ggcgaggacg
     5581 cgtcggccgc gctcgacgag ctgccggtag aggccatcgc gtacgtccat gtggcgggcg
     5641 gggccacccg cgacggcgtc tggcacgaca gccacggcca cccggtcccc cagcccgtcc
     5701 tggacattct ggccgacctc gcgtcccggg tgacaccacc gggcgtactg ctggagcgcg
     5761 acgagaactt cccggcccag gccgagctgg agcgcgaact cgacaccctc cgcgcggccg
     5821 tgtcccggga ccggctgctg gccacggcgg gaccgtcggc gggcgtccca gccggacgac
     5881 ggccgggggt cacgtccaag cgaccgtcag gcgacacaac ccgacggccg ccgtcaaccg
     5941 tcacgaccgg ccgaccgtca ggcgcaccgc tggccttggc caccggcccc gcttggcggc
     6001 cggttcaggg cacggccccg gccgcggtcc ccgccacggc cgccacacga gacgtaccgg
     6061 acgcaccgga cgcaccggcc caggcgagcg acgccgcgcg ccagcgggtc gcgctgcgcc
     6121 aggcgtcgct gctctccgcg ctggtggcgg ggacgccccc gcccgaaggg ttcgacggcc
     6181 agaggctgcg cgtacagagc cgggcgctga ccgcgaagcg ggcctccgtg gtggccaagg
     6241 tggcgcccga gctgccggag atcctgggcg cggagtaccg gtccgccttc ctggcgtacg
     6301 ccaggaaccg gccactgacc gccgggcacc ggcgcgacgc gctggccttc gccgagcacc
     6361 tgctgcgcgc ccgcaagccc cgctcgcccc gcgcgcggcg ggagttggtc cggtggtggc
     6421 tggagcgctc gggtccggcc ccgctcaccg gccgcccgct ggcgcggctg gccaggacgg
     6481 cccgctacgc gttgctacgg aggtgacggg ctcgccacac cgagcatcgg ggttatggcg
     6541 gcgacaccgg ccgcccggtg ctttacttcg cccttcaccg cccgaactat ccctttttat
     6601 acagccgcag tccgctgccg ttcgaaggga tgccgagcga tgtcgaagca cggaaagcgc
     6661 ggaagagcgc ctctcctgta cgccgtgacc ggatcgctgg ttctgaccgg cctggccgcg
     6721 tcgcccgcgt ccccggcggc ctccgcgagc agcacgcccg aggcccccga ggcccgtggc
     6781 accagggtcg ccgtcgcacg cgccgaggcg gccgggatct ccttcggccg ctgccccgag
     6841 gtcgagaggc tgcccgcggc cgtacagtgc ggcacggtga ccgtaccgct cgactacgcc
     6901 gaccccgacg gcaagcagat caaactgacg gtcagccggg tgaaggccaa ggacccggcc
     6961 aagcgccagg gcgccctgat cttcaacccc ggtggccccg gcggctccgg gatgtacttc
     7021 ccggtggtct cgacggccgg gctgcccgct ttccggcgca tcgcgcggga gtacgacgtc
     7081 gtcggctacg cgccacgcgg agtcggccgc tccgccccgg tgtcctgcaa ggacccggcc
     7141 acgttcgacc ccgcgccgac ccggtcaccg cgccaccccg acgcggcgta caaacaggag
     7201 cgcgtcgcgc gggcgaagac gtacgcgcga ggctgcctga accggaccgg ggaggcactg
     7261 cggcactacc attcgctcaa caacgcgcgc gacctcgacg tgctgcgcgc cgggctcggc
     7321 gagcggaagc tgaacttcct gggcgcctcg tacgggacct acttcggtgc cctgtacgcg
     7381 gcgatgttcc cctcccatgt gcgccggatg gtcttcgact ccgcggtcaa ccccgacccg
     7441 tcgcagatct ggtaccagaa caacctcgac cagtcgctgg cgttcgagcg ccgctgggcc
     7501 gacttccgcg cgtgggtcgc ggcgcatcac acgacgtacg gcctgggccg cactccgcag
     7561 caggtggcgc actcctacga gaaggtgcgg gccgcgctgg cgcgcgaacc ggcgggcggc
     7621 aaggtcggca ccggtgaact ccaggcggcg tttctgcaga ccgggtacca cgacgacgtc
     7681 tggcccaagc gggccgccgc gctgaccgcg tatctgaacg gcgacccgaa gccgctcgtc
     7741 cagcaggccg cgcgctcttc ggagcgggcg gccgagaacg agaacggcaa cgccgtctac
     7801 accgccgtcg aatgcaacga cgcgccctgg ccgacggact tcgagacctg ggacgaggac
     7861 aactcccggc tcgcgcgcgt ggcgcccttc gagacctggg acaacgcctg gatgaacctg
     7921 ccgtgcgcct actggccggt gccccggcag cgccccctgg acctccgcat ccccgacggc
     7981 gccctgcccc cgaccctgat cctggccgcc gagcgggacg ccgcgacccc gtaccagggg
     8041 gcgtgggagc tgcatcgcag gctcccggac tccgcgctgg tcaccgagcg caaggcgggt
     8101 acgcacggcc tcgtcggcgg ccccaaccag tgcgtcaacg ggcacctgga ggcctacctg
     8161 ctggacggcc gcctcccggc acggcgcgcg gcgggctgcg caccccgccc agagcccaag
     8221 cccgagggcg agccggaggc agcgggcagc cgagaggcca cccgcacgcc ccggaccgca
     8281 tagggcatcg accgcttcgg cacagaccgc gtagggcagg cctcttaggc cagccccgcc
     8341 accaggtcgg ccaccgactt gcggcgcccg gtgtagaacg gcacctcctc gcgtacgtgc
     8401 atccgtgcct cggacgcgcg caggtgccgc atcaggtcca cgatgcggta cagctcgtcg
     8461 gcctcgaagg cgagcagcca ctcgtagtcg ccgagcgaga aggacgccac ggtgttggcg
     8521 cggacgtccg ggtagccgcg ggccatcttg ccgtggtccg cgagcatcct gcggcggtcc
     8581 tcgtcgggca gcaggtacca gtcgtaggag cgcacgaacg ggtagacgct gacgtagtta
     8641 cgcggcgtct cgtcggccag gaaggccggg atgtgcgact tgttgaactc cgcggggcgg
     8701 tgcagcgcca tgttcgacca gaccggctcc agggcgcggc ccagcttggt gcggcggaag
     8761 aggttgtacg cctcctggag ctcgtcggcg gtctcggcgt gccaccagat catcagatcg
     8821 gcgtcggcgc gcaggcctga cacgtcgtag gtgccacgga cggtgatgtc cttggcggcg
     8881 agctggtcga acagttcctg gacctcgtcg gcgtagccgg tccggtcttc gggcagtacg
     8941 tcgcgcagct tgaagacgga ccacagcgtg tagcggatga cctcgttgag gtccttggcc
     9001 ttcttacctg cgttcgggct cttggcgggg gcgtcgtcac tcatggggct attctgccgc
     9061 gccgccgtgg agactctgga ccgggttccc ggtgagctga tccagagcgc tggaatcacc
     9121 gcccagctgg tccacggccg cgtacgcgct ggcgatgcag gccgggatgc cgaccccgtc
     9181 gtacgcggcg ccgcagaccg ccagtccggg cagcttggcg acgtgctcgc ggatgcgcgc
     9241 cacgcgcgcg tggtgcccga cggggtactg gggcagcccg tcgttccatc gggtcacgcg
     9301 ggtggcgacg ggcgtggcgg tcagtccggt cgcctcgcgc aggtcgcgcc gcgatacgtc
     9361 gatgagctcg gcgtcgtccc ggcgcagatc gcgctcctcg ccataccggc cgaccgaggt
     9421 gcgcagcacg aacacgtccg ggtcctcgtc ggcgatccag ccccacttct ggctagcgaa
     9481 ggtggacgcc ttgatggtgt gcccgtcgac tggcggcacc aggaagccgc tgcctgccgg
     9541 gagattcgcg tcggtcctgc ggtaggcgag ggtcatcagc gccatggagg cgtactcgac
     9601 ggcgcgcagt tcggccgctg cggcgggcac gtcggcgtcc aggaggcggg cggcctgggg
     9661 ggcggggacg gcgatgacga cggcgtcggc cttgatgccc ccgtcctcgc cgttctcgat
     9721 gtcgacctcc cagccgtccg cgacccggcg cactccgcgc accggacagt tgagccggat
     9781 ctcggcgcca cgcgcgcgta gcgcgtcggc gacggcgagg gggagtccgc cgacgccgcc
     9841 cgcgatgccc atgaacaccg gtccggtggc cccagcctcg acggcctggc gctggatgtc
     9901 gcgcacgccg tccaggaggg agcggtgggt gcgggcggcc tcgtagagct tgggtacggc
     9961 catccgcatc gagaggcggt acgcgtcgcc cgcgtacacc ccgccgagca gcggctccac
    10021 caggcggtcg acgacctcac ggccgacgcg ggacgcgacg tactcgccga ccgcgatgtc
    10081 gtcgccgacc tcggtcggcg gcaactcccg gtcctgctcg atccgacgca gcccctcgtc
    10141 ggagagcacc ccggccagcg cgccggcgtc gccgggtacg cccatcacat gtcccttggg
    10201 catggggcgc agctcgccgc gcgtccagag tgcggcggtc gcggtggcgg gcggctggag
    10261 caggtcgccc aggcccaccg cgcgggccag ctcgacggct tcggggcggc gggccagcat
    10321 cgactcggcg ccgaggtcga cgcgtacgcc ctcgatctca ccggcgtgca gcttgccacc
    10381 gagccggtcg gcggcctcca ggacggtcac ccgggcgccg gtgccgagca atcggtgggc
    10441 ggcggcgaga ccggcgatcc cgcctccgat gacgacgaca tggctcgtcc gcgtgtccgt
    10501 tttccgcatg gtttcactct ctcagacccg gatcgggagt ccgtaccgag tccacaccgt
    10561 gaccgcttcg gtaccgctgc ggaccaaacc acccgcgggt cgcgcgcgtc aaagccgtat
    10621 cggacaccac atctccctgg ggggcctcgt caccatgcgc gcatcgaccc gttcgcgttc
    10681 ccgtttccgc tcgcgttccc gttctcgtcg ccagtccacc cgggccctga ccgccttgct
    10741 gctcgcggcg gccgtcgcgg tcggggggtg cagcggcgac cgcggctcag acggagcaaa
    10801 gtcgaggagt gacagcaaag ccggggccgc ggaggacgcg gcgggtggcg accaggcagc
    10861 ctctggctcc ggctccaagt ccgccgaccg gcaggcgccc gaccctgtcg gaacccacat
    10921 catccgcacc gccacgctga ccgtacgagt caaggacgtg ccgaaggccc tgggcgcggc
    10981 ccgcgacgcc accctcgacg cgggcggcgg cgtcggcagt gagaccacca accgcgaccg
    11041 gcagggccgg gagcgctcgc ggctggtgct gcgggttccg caggcgaagt accacgacgt
    11101 cctgcgggcc ctggcgggca cgggcaaggt ggtggaacgc aagctcaagg aggagaacgt
    11161 caccgagcag gtggtggacg tcgagagccg cgtcaagtca cagcgcgcca gcgtcgcccg
    11221 ggtgcgggag ctgatggacc aggccaccaa gctcagtgat gtggtgacgc tggagggcga
    11281 gttgagctcg cggcaggccg acctggagtc gctgctggcc cggcaggcct cgctcaagga
    11341 ccgtgtcacc ctggcgacga tcacgctcca actgaacgag accgggcaga agcagcaggc
    11401 ggacaaggac gacgacccca cgttcgtcag tgcgctgaag ggcggctgga acgcgttcgt
    11461 caccggtctg cgctggatca ccatcgcgct cggcgcggtg cttccg
//
