LOCUS       NZ_WHOM01000059         2685 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 501, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000059
VERSION     NZ_WHOM01000059.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2685
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2685
                     /collection_date="2018-07"
                     /db_xref="taxon:2654677"
                     /geo_loc_name="China: Xinjiang"
                     /host="Apocynum hendersonii"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces apocyni"
                     /strain="TRM 66233"
                     /type_material="type strain of Streptomyces apocyni"
     gene            complement(<1..>2685)
                     /locus_tag="GCN81_RS16500"
     region          1..2685
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2685
                     /SMILES="NC(C(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2685
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2685](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2685)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..2685)
                     /NRPS_PKS="Domain: AMP-binding (188-587). E-value:
                     3.2e-107. Score: 350.6. Matches aSDomain:
                     nrpspksdomains_GCN81_RS16500_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (695-760). E-value: 2.6e-23. Score:
                     74.0. Matches aSDomain: nrpspksdomains_GCN81_RS16500_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099845.1"
                     /locus_tag="GCN81_RS16500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_156725393.1"
                     /sec_met_domain="AMP-binding (E-value: 3.5e-121, bitscore:
                     390.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.3e-16, bitscore:
                     44.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.3e-10, bitscore:
                     25.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLVGFFINTLVLRTDTSGNPTFRELLNRTRTTNLTAYAHQDLPF
                     ERLVEALNPERSLTRHPLVQTMFTLQNAPGGRLELPGIDARLEPSGLSAAKFDLSIEVG
                     ERPGDGDTPDAFEGVVHYSVDLFDRATVETLMARWTRLLASLVAAPDRPLGEIDVLTAD
                     ERDRLADWNHTHHEVPEVSGTLRARFAAQVARTPEAVAVHSAASELTYRQLDERANRLA
                     HRLVGLGVGAETCVAILQERSADLLVSILAVIKAGGAYVPLDRRFPASRHQLITAETGC
                     RVLLTDAASWADGFAHDAVTVVVDAEAELAELATRPATDPVVPGHPSNAAYVMYTSGST
                     GTPKGVVVTHRDVLSLVYDRCWGDGVSDRVLVHSPQAFDASTFELWVPLLSGGQAIVAP
                     PGELDLRALELVITRERVTGLWLTAGLFRLLAEERPESLAGVREVWTGGDVVPPASVRR
                     VLEAAPDTVVVDGYGPTETTTFATHHKVGAADSVPAAMPIGRPLDNMRVHVLDEALRQV
                     PPGVPGELYIAGAGLARGYVNRAGLTAERFVADPYGTAGERMYRTGDLVRWNAQGHLEY
                     LGRGDDQVKLRGFRIELGEIEAALTAHPGIAQAMVLVREDRPGDKRLTAYVVPVAGHAA
                     PQTAELRDHLDQSLPAYMVPAVFVPLDALPLTPNGKVDRKALPAPDYTALTTGREPRTP
                     EEKLLAGLFADVLGLPSVGVDDSFFDLGGDSIISIQLVSRARKAGLVFTPRDVFQRKTV
                     AGLASVAMPADTAVVEDPDAGIGDIPLTPIMHWLRERPGPIDGSHQSMLVRLPAGLGED
                     RLASALQTVLDHHDALRARLVRTGSDVWSLEVAERGTVQARECVQRVDTTGLDSDGVRD
                     VLVREAEAARSRLAPEAGAMVQAV"
     CDS_motif       complement(187..255)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0008"
                     /evalue="1.80E-05"
                     /label="C2_starter_024-063"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="833"
                     /protein_start="810"
                     /score="17.4"
                     /tool="antismash"
                     /translation="EDRLASALQTVLDHHDALRARLV"
     aSDomain        complement(406..600)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS16500_PCP.1"
                     /evalue="2.60E-23"
                     /label="GCN81_RS16500_PCP.1"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="760"
                     /protein_start="695"
                     /score="74.0"
                     /tool="antismash"
                     /translation="EKLLAGLFADVLGLPSVGVDDSFFDLGGDSIISIQLVSRARKAGL
                     VFTPRDVFQRKTVAGLASVA"
     aSModule        complement(406..2121)
                     /complete
                     /domains="nrpspksdomains_GCN81_RS16500_AMP-binding.1"
                     /domains="nrpspksdomains_GCN81_RS16500_PCP.1"
                     /locus_tags="GCN81_RS16500"
                     /monomer_pairings="Val -> Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(448..477)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0007"
                     /evalue="2.60E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="746"
                     /protein_start="736"
                     /score="-0.6"
                     /tool="antismash"
                     /translation="KAGLVFTPRD"
     CDS_motif       complement(565..627)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0006"
                     /evalue="7.00E+00"
                     /label="C5_LCL_267-296"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="707"
                     /protein_start="686"
                     /score="-0.7"
                     /tool="antismash"
                     /translation="TGREPRTPEEKLLAGLFADVL"
     CDS_motif       complement(895..960)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0005"
                     /evalue="1.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="597"
                     /protein_start="575"
                     /score="39.1"
                     /tool="antismash"
                     /translation="YLGRGDDQVKLRGFRIELGEIE"
     aSDomain        complement(925..2121)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS16500_AMP-binding.1"
                     /evalue="3.20E-107"
                     /label="GCN81_RS16500_AMP-binding.1"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="587"
                     /protein_start="188"
                     /score="350.6"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FAAQVARTPEAVAVHSAASELTYRQLDERANRLAHRLVGLGVGAE
                     TCVAILQERSADLLVSILAVIKAGGAYVPLDRRFPASRHQLITAETGCRVLLTDAASWA
                     DGFAHDAVTVVVDAEAELAELATRPATDPVVPGHPSNAAYVMYTSGSTGTPKGVVVTHR
                     DVLSLVYDRCWGDGVSDRVLVHSPQAFDASTFELWVPLLSGGQAIVAPPGELDLRALEL
                     VITRERVTGLWLTAGLFRLLAEERPESLAGVREVWTGGDVVPPASVRRVLEAAPDTVVV
                     DGYGPTETTTFATHHKVGAADSVPAAMPIGRPLDNMRVHVLDEALRQVPPGVPGELYIA
                     GAGLARGYVNRAGLTAERFVADPYGTAGERMYRTGDLVRWNAQGHLEYLGRGDDQVKLR
                     "
     CDS_motif       complement(1045..1134)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0004"
                     /evalue="2.70E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="547"
                     /protein_start="517"
                     /score="54.8"
                     /tool="antismash"
                     /translation="PPGVPGELYIAGAGLARGYVNRAGLTAERF"
     CDS_motif       complement(1636..1695)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0003"
                     /evalue="9.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="350"
                     /protein_start="330"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYVMYTSGSTGTPKGVVVTH"
     CDS_motif       complement(1894..1935)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0002"
                     /evalue="6.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="264"
                     /protein_start="250"
                     /score="19.2"
                     /tool="antismash"
                     /translation="LAVIKAGGAYVPLD"
     CDS_motif       complement(2476..2595)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS16500_0001"
                     /evalue="3.40E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="GCN81_RS16500"
                     /protein_end="70"
                     /protein_start="30"
                     /score="77.3"
                     /tool="antismash"
                     /translation="RTTNLTAYAHQDLPFERLVEALNPERSLTRHPLVQTMFTL"
ORIGIN
        1 cacggcctgc accatcgccc cggcctccgg ggcgagcctg ctccgggcgg cctcggcctc
       61 ccgcaccagc acatcccgca ccccgtcact gtccaggccg gtggtgtcca cccgctggac
      121 gcactcacgc gcctgcacag tgccgcgctc ggcgacctcc aatgaccaca cgtcactgcc
      181 cgtacgtacg agtcgggcac gcagcgcatc gtggtggtcc agaacggtct ggagcgccga
      241 cgcgagccgg tcctcgccca gccccgcagg caaccgcacc agcatcgact ggtgcgagcc
      301 gtcgatcggc ccggggcgtt cgcggagcca gtgcatgatc ggtgtgagcg ggatgtcgcc
      361 gatgcccgcg tccgggtctt ccaccaccgc ggtgtcggcg ggcatcgcca cgctggccag
      421 gcccgcgaca gtcttgcgct ggaacacatc gcgcggagtg aacaccagac cggccttgcg
      481 ggcccggctg accagctgga tggagatgat gctgtcgccg cccaggtcga agaaactgtc
      541 gtccacaccc acggacggca gtccgaggac gtcggcgaac agcccggcga gcagcttctc
      601 ttccggggta cggggttcgc gcccggtcgt gagagcggtg tagtcgggcg cgggcagcgc
      661 cttgcggtcc accttgccgt tcggggtcag cggcagcgcg tccaacggca cgaagaccgc
      721 cgggaccatg tacgcgggca ggctctggtc caggtggtca cgcagttccg cggtctgcgg
      781 cgcggcgtgc ccggccacgg ggacgacgta cgcggtcagg cgcttgtcgc ccggccggtc
      841 ctcccgtacc aggaccatcg cctgggcgat gcccggatgc gcggtcagcg cggcctcgat
      901 ctcgcccagc tcgatccgga acccgcggag cttgacctgg tcgtcgccgc gacccaagta
      961 ctcgaggtgg ccctgggcgt tccagcggac caggtcgccg gtgcggtaca tccgctcgcc
     1021 cgcagtcccg taggggtcgg cgacgaagcg ttcggcggtc aggcccgcac ggttcacata
     1081 gccgcgcgcc agcccggccc cggcgatata cagctcgccc ggcacaccgg gcggcacctg
     1141 gcgcagtgcc tcgtccagca cgtgcacccg catgttgtcc agcggacgcc cgatcggcat
     1201 ggccgccgga acactgtccg ccgcgccgac cttgtggtgg gtggcgaacg tggtggtctc
     1261 ggtcggtccg tagccgtcga ccacgaccgt gtccggcgcg gcctccagca cgcggcgtac
     1321 ggaagcgggg ggaacgacgt cgccgccggt ccacacctcc cgtacgccgg ccaggctctc
     1381 gggccgttcc tcggccagca gtcggaacag gccggccgtc agccacaggc cggtcacccg
     1441 ctcccgcgtg atgaccagct ccagcgcccg gaggtccagc tcgcccggcg gggcgacgat
     1501 cgcctggccg ccggacagca acggcaccca cagctcgaat gtcgaggcgt cgaaggcctg
     1561 cggggagtgc accaggaccc ggtcgctcac cccatcgccc cagcagcggt cgtacacgag
     1621 gctcagcacg tcccggtgcg tgacgaccac acccttgggc gtgccggtcg aaccggaggt
     1681 gtacatcacg tacgccgcgt tggacgggtg ccctgggaca acggggtcgg tggcgggccg
     1741 cgtcgccagc tcggccagct ccgcttcggc gtccacgacg acggtgaccg cgtcatgggc
     1801 gaagccgtcg gcccacgagg ccgcgtcggt cagcagcacg cggcagccgg tctcggcggt
     1861 gatcagctgg tgccgggagg cggggaagcg ccggtcgagc ggcacgtacg cgccaccggc
     1921 cttgatgacc gccaggatcg acacgaggag gtccgccgaa cgctcctgga ggatcgccac
     1981 gcaggtctcc gcgccgacgc cgagccccac gagccggtgc gcgagccggt tggcccgctc
     2041 gtccagctgg cggtatgtca gctcgctcgc tgccgagtgc accgcgaccg cctcaggcgt
     2101 tcgggcgacc tgcgcggcga acctcgcccg gagcgtgccg gagacctcag ggacctcgtg
     2161 atgggtgtgg ttccagtcag ccagccggtc ccgctcgtcg gcggtcagga cgtcgatctc
     2221 cccgagcgga cggtcgggcg cggccacgag cgacgcgagc agccgcgtcc agcgggccat
     2281 cagggtctcg accgtcgccc ggtcgaacag atcgacgctg tagtggacga caccctcgaa
     2341 cgcgtcaggc gtatcgccgt ctccgggccg ctcgcccacc tcgatcgaca ggtcgaactt
     2401 cgccgcgctg aggccggacg gttccagacg ggcgtcgatg ccgggcagct ccaggcgccc
     2461 accgggggcg ttctggaggg tgaacatggt ttggacgagg gggtggcggg tgagggagcg
     2521 ctcggggttg agggcttcta cgaggcgttc gaaggggagg tcttggtgtg cgtaggcggt
     2581 gaggttggtg gtgcgggtgc ggttgaggag ttcgcggaag gtggggttgc cgctggtgtc
     2641 ggtgcgcagg acgagggtgt tgatgaagaa gccgacgagg tggtc
//
