LOCUS       NZ_WHOM01000053        21107 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 491, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000053
VERSION     NZ_WHOM01000053.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 33703)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33703)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4615
            Orig. end                         :: 25722
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21107
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21107
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21107
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:11107](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11107
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    464..478
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.12"
                     /tool="antismash"
     misc_feature    839..853
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.38"
                     /tool="antismash"
     misc_feature    1432..1447
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.51"
                     /tool="antismash"
     misc_feature    complement(1677..1690)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    complement(2333..2348)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     misc_feature    2425..2440
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            2516..2785
                     /locus_tag="GCN81_RS14785"
     CDS             2516..2785
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060729.1"
                     /locus_tag="GCN81_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156725071.1"
                     /transl_table=11
                     /translation="MATTTYLVRDAYSKRQETRDLPDLSLVSGPDSGLLGSCSCGCLEP
                     PLTSEPPLADAAPLTSEPPLADAAPLTSEPTATGTASFLDPSAY"
     gene            2786..5038
                     /gene="malQ"
                     /locus_tag="GCN81_RS14790"
     CDS             2786..5038
                     /EC_number="2.4.1.25"
                     /GO_function="GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="malQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137305.1"
                     /locus_tag="GCN81_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4-alpha-glucanotransferase"
                     /protein_id="WP_156725072.1"
                     /transl_table=11
                     /translation="MPRPREREPGAEPLDLLAARYGVATSYEPSPGRTVRAEPAAVVAV
                     LAALGVDASTPDAIRAALTAREAELRERLLPPTLVAWAPVGPVGPVASGEPVAPVEPSP
                     ELVSPPLGALEKLAGLPTGTRLWVRTEQGDAAGWVVPEASGGCGAPGTSEAAGVSGASG
                     DGVGGPRLPLGVHEVGAEAPDGRRAQAYLVVAPERMPAPGERAHGLLVQLYSLLSRRSW
                     GMGDLGDLADLAAWSGRALGTGFVQVNPLHASVPGGPGGPTDPSPYRPSSRRFPDPVHL
                     RVEDIPEYVYLAPEARQEAAELLARAAILRQGVLNEGALIDRDAVWALKSRALELLREV
                     PLSPGRRAAYCDFLAEQGEPLEAYATWCALAERYGSDWPSWPSPLRDPASPGTARARAG
                     LMERIDFHCWLGWLTDAQLAAAQRAALDAGMAIGLVHDLAVGVHPGGADAWARREFFAA
                     GMSVGAPPDAFSADGQDWGLPPWRPDALAASGYAPFRDLLRGLLRHAGALRIDHVMGLF
                     RLWWVPAGRPPTEGAYVHYDADAMLAVLALEAHRAGAAVIGEDLGTVAPGVREALAARG
                     VLGTSVLWFERDWDGTGRPLPPDRWRADCLATVTTHDLPPTAARLSGEHIRLHQRLGLL
                     TGPLSRATAEGAAETGEWLAVLARLGLLPGGRGARGEEEKIRAVYRFLARTPARMVGVW
                     LPDAVGDRRPQNVPGTWRTYPNWRLPIADATGRAVTLEELAASPRLHRLMAELRGL"
     misc_feature    3302..3304
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3536..3538
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4868..4870
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5097..5348
                     /locus_tag="GCN81_RS14795"
     CDS             5097..5348
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886343.1"
                     /locus_tag="GCN81_RS14795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156725073.1"
                     /transl_table=11
                     /translation="MDKKNALRAGAVAAGTTLMMLLMTSPALALTRDDGDDPAPKLSVM
                     ETLGLYVAAPIALFLLIAGLVMVGDKSKDRQPKPQKQG"
     misc_feature    complement(5454..5468)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.37"
                     /tool="antismash"
     misc_feature    complement(5461..5475)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.21"
                     /tool="antismash"
     gene            5570..6550
                     /locus_tag="GCN81_RS14800"
     CDS             5570..6550
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886339.1"
                     /locus_tag="GCN81_RS14800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_156725074.1"
                     /transl_table=11
                     /translation="MAILDHVRGSEPTERVRRRSTGPRPGKRRAAQAASAASASGLGLV
                     LAALATVVWSGSFVTSRALADTVPPVQHAFWRWIIALIAVAPFATRAAWRQRDLIRRHI
                     GYLTLAAFLGVATYNTLVNQAGISTSAGNMAMIMAASPVLMALYERLGGTRLGARRALG
                     MLIACAGVLLLVSKGSLAMDFTTGDLWMLAAALSFASYSALLRGRPAGLGGLAFLFTTF
                     ALGAVMLAPCYGVSLAVQGGFEVTTGTVGPLLYVGVVSSAVAFFAWNKAITLIGAARAG
                     VIYYLQPVCVAALSFAILGESVGVLQGACMALILGGVILASARAR"
     gene            6648..7973
                     /locus_tag="GCN81_RS14805"
     CDS             6648..7973
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542843.1"
                     /locus_tag="GCN81_RS14805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_231626730.1"
                     /sec_met_domain="p450 (E-value: 1e-30, bitscore: 97.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLVDPAALDPAALDSALLDPASLAPAPAPAPALLDPADADFAR
                     DPYPYYARLRAQGPAVRTPLSNGTHAWLVTGYQAARAVLADPRFSNVPAPRAGRPKPDS
                     AAERARACLARHMLNLDAPDHNRLRRLVTGAFTPRRVDALRPRIEGLVGPLVADVRARL
                     DRDGVADLVDTFAFPLPVLVIAELLGIPEADRTSLREWTYRVGSPADSLAPGEVEEAWG
                     SLHGYFTELIAQKRRSPGDDLFSVLVHEEREGALDDGELLALAFLLLFAGYETTMNLLS
                     SAALLLITHRDELAAARREGTRRWDAVVEETLRHASPLEGATWRRTTEEVDLGAGALIP
                     AGESVLVVLAAASRDPASFDAPDDFRPARYLSGAGRAAGHMAFGHGAHVCLGARLARLE
                     AGVALPMLFDALPKLGLADDVAEVPYRPGLLVRGPRVLRVRT"
     gene            8005..8985
                     /locus_tag="GCN81_RS14810"
     CDS             8005..8985
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886337.1"
                     /locus_tag="GCN81_RS14810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_156725075.1"
                     /transl_table=11
                     /translation="MSEWDIKKLRILRTLSERGTVTATAQELLMTPSAVSQQLSSLARQ
                     LGVPLLEARGRRVRLTDAAHLVLRHAEVVFAQLERADGELAAYARGEAGEVRVGAFSTA
                     VPALVVPAVRHLRTEAPALEVRVREAEAAQAYELLAGGAVDLALSLAAHAPTARDPKFT
                     RVPLLADPLDVALPVDHPLAGAESVRLADLAEEQWIFGGSGPWSEITTTACEAAGFVPE
                     QAHSAAGWTAILAMVEAGMGVALVPRMAVAGRQSGVTVRVLTGDRPRRHVVAAVRRGAD
                     QAPTVARVLTALQAAAPGHHEVNARSATGWGTGHGLPPRVRKTTG"
     gene            9095..9997
                     /locus_tag="GCN81_RS14815"
     CDS             9095..9997
                     /NRPS_PKS="Domain: oMT (82-178). E-value: 3.5e-08. Score:
                     23.8. Matches aSDomain: nrpspksdomains_GCN81_RS14815_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886335.1"
                     /locus_tag="GCN81_RS14815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_156725076.1"
                     /transl_table=11
                     /translation="MNRSLTPFDESQVPLYYSRKTADILYKYGPGPRVHYHMGLFEPGA
                     APNTTVSQHSLKRRIVASQEAIVEHAARSWGAHQAAPGSVLDIGCGLGGGSIYWAQEHG
                     ATVTGLTVAAEHLPVIAGFARQAGVAGQVTPLLADVHQYTADRLFDAAYANESSGYMDR
                     ERLFQVVAKSLKPGGWFGIQEHFICRPEWTEFIDGYYKTRLGTLTEYITAAEAAGFELE
                     QDEDVTDRVAEFWVQSMAWNTAELERVERGGAPERLAWSRERLTESALTHGKLFRIWRD
                     HALETRLLMFRLAGGPGGR"
     aSDomain        9341..9628
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS14815_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="3.50E-08"
                     /label="GCN81_RS14815_oMT.1"
                     /locus_tag="GCN81_RS14815"
                     /protein_end="178"
                     /protein_start="82"
                     /score="23.8"
                     /tool="antismash"
                     /translation="GSVLDIGCGLGGGSIYWAQEHGATVTGLTVAAEHLPVIAGFARQA
                     GVAGQVTPLLADVHQYTADRLFDAAYANESSGYMDRERLFQVVAKSLKPGG"
     gene            10001..11107
                     /locus_tag="GCN81_RS14820"
     CDS             10001..11107
                     /GO_function="GO:0010333 - terpene synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886333.1"
                     /locus_tag="GCN81_RS14820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="terpene synthase family protein"
                     /protein_id="WP_156725077.1"
                     /sec_met_domain="T1TS (E-value: 1.3e-70, bitscore: 229.6,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPVYAADTAPSRIGWELPPFYCPFNEQLIHPKAAELETRAIEWI
                     DAFGIYSDATERAWGLATHSTDFTSRIIPYGDVEPILLFVEWNYWANAVDDWQDSGDTA
                     TRTAAIADHSVRLVRSIEAPGSSLLPPGPLTTALDDLVARTRAMLTPSQLRRFTEGTRD
                     WLFGAGWQTANAELDVMPSLNDFCAMRMSVNGTRFTLTWCDAANGIHVPADVLYSAPVQ
                     ALTDAAGFIVSCDNDLFSYNKEDHLNPWEQNLLNVVAQHQGCTPAEAMPAAVALRDRAM
                     TLFVRLREQLYAGAGDDLRRYLDSLGHYVSGCIEWQNLAPRYTSPRNRNALPVEGASYD
                     VTWRDTPSDPEQGPPPIPAIAWWWAQLR"
     gene            complement(11160..12584)
                     /locus_tag="GCN81_RS14825"
     CDS             complement(11160..12584)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735524.1"
                     /locus_tag="GCN81_RS14825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_156725078.1"
                     /transl_table=11
                     /translation="MTSPEPATPVPSGGTPPPGCPAHAMSPEGLRRLYGPEAEADPRGL
                     YEALRAEHGTVAPVLVQGDLPAWLVLGHRENLDVARTPSRFSRDSRRWRDMQEGNVPAD
                     HPLTPVAAWQPVCNFADGTDHERLRGAVTESLERFDRRGIRRYVTRFAGQLIDKFAEAG
                     RADLVADFAEPLPMLVMTQLVGAPDSYGPRLVQAARDMTRGTDTAIASNEYLSVALRQL
                     LEAKRASPGHDFTSWLLEHSASLTDAEIIEHLRLVVIAAFETTANLIANTLRMMLTDRR
                     FRAHLSGGHMTLPDALEQVLWDEPPLTTILGRWATGDTMLGGQRIKAGDMLLLGLAAGN
                     TDPEIRPDLTSPVHGNRSHLAFSGGPHECPGQDVGRAIADTGIDALTSRLPDLRLGVPE
                     DELRYDSTLMSRRLVALPVTFTPLGSRTGATPLPRPPVAEPLPAAAAPPPAAPPPAALA
                     PEPVRRSWWARLFARR"
     gene            complement(12581..13201)
                     /locus_tag="GCN81_RS14830"
     CDS             complement(12581..13201)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944873.1"
                     /locus_tag="GCN81_RS14830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_156725079.1"
                     /transl_table=11
                     /translation="MDFRSSEPTVGPRSEDVLPETATAAVKVVIVGGFGVGKTTLVGSV
                     SEIRPLTTEETMTQAGVGVDDTAGVERKSTTTVAMDFGRISINDELVLYLFGTPGQERF
                     WFLWRGLFEGALGAVVLVDTRRLEVSFDVIGRLEERGVPFVVAVNSFPDAPEHPIEELR
                     GALDLPASVPIVACDARSRASSRDVLMALMRYLQSLAATTEAS"
     gene            complement(13182..13547)
                     /locus_tag="GCN81_RS14835"
     CDS             complement(13182..13547)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944874.1"
                     /locus_tag="GCN81_RS14835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_156725080.1"
                     /transl_table=11
                     /translation="MTQPGPGQAWDEGGPERLYVITGGRSGSSAPTALDLVTLIVATSG
                     PGPGMQPEQAAIVRLCQRPLSVAEISAYLGLPVSVVTVLLSDLLADQRVTSRPPVPPAK
                     LPDIALIEAVIDGLQKL"
     gene            complement(13544..13933)
                     /locus_tag="GCN81_RS14840"
     CDS             complement(13544..13933)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474920.1"
                     /locus_tag="GCN81_RS14840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="WP_156725090.1"
                     /transl_table=11
                     /translation="MDWMLKDLAEGVPQTRQVVVLSADGLRMAQFGADTDSADRLAAAC
                     AGLQSLAGAVAAELPDSDGQMRLVVIEMNGGFFYLMAAGAGAYLAVLADEGVDAGLMGQ
                     RMRDLVLRIGEHLSSPPRQDGQAAR"
     gene            complement(13978..15546)
                     /locus_tag="GCN81_RS14845"
     CDS             complement(13978..15546)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712912.1"
                     /locus_tag="GCN81_RS14845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_407697971.1"
                     /transl_table=11
                     /translation="MQRVRTGTSPGSPKSPFPLWLLPPAAAAALTALAAALLDSGRAAV
                     LVTGGAAVVGLAVACAIAARRGREIAELRATAAERDEALARREAETVRLAEELLPDVVA
                     RLRKGEFPEDVLDSLSAPAAHLAVLRSVVDAVTAEEDLRDSAQRAFVNIARRVQAIVHQ
                     QAQELREMEDRHGGHPEVFGDLLRIDHGTALIGRLADSIAVLGGARPGRQWSRAVPLYS
                     VLRGSMSRIIDYQRVELHSVSEVAVVGTAVEPLIHALAELLDNATRYSPPQTRVHLTAV
                     DVQSGIAIEIEDGGVSMSEEARGRAERMLKQAQQGIDVNDLGETPRLGLAVVGRLSQVY
                     GFQVSLRPSAYGGVRAVLVVPQDLITTAAEATGLAHGIGTASGPRASAEPSRAAAGTAR
                     TAARTAAGTAPRPVTGPAPVTDDAPVVAERTANGLPQRRRRPTAATPTASAAATSAAAA
                     TPPESAPSEPAPPVQPGIWLAAFQSGLSGESAQAVTDPEHTTQAGPAGQDHDASEASAS
                     KGNEQ"
     gene            complement(15822..18392)
                     /gene="pepN"
                     /locus_tag="GCN81_RS14850"
     CDS             complement(15822..18392)
                     /EC_number="3.4.11.2"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="pepN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527627.1"
                     /locus_tag="GCN81_RS14850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminopeptidase N"
                     /protein_id="WP_156725081.1"
                     /transl_table=11
                     /translation="MPGTNLTREEAQQRAKLLTVDAYEIDLDLSGAQEGGTYRSVTTVR
                     FESAEAGAETFIDLVAPTVHQVVLNGDALDTAAVFADSRITLANLLQGPNELKVVADCA
                     YTNTGEGLHRFVDPVDDQAYLYTQFEVPDARRVFASFEQPDLKATFQFTVKAPSGWTVI
                     SNSPTPEPKDDVWVFAPTPRISTYITALIVGPYHSVHSTYDGPGRSVPLGIYCRPSLAE
                     YLDADAIFEVTRQGFDWFQEKFDYAYPFEKYDQLFVPEFNAGAMENAGAVTIRDQYVFR
                     SKVTDAAYEMRAETILHELAHMWFGDLVTMEWWNDLWLNESFATYTSIACQAYAPDSKW
                     PHSWTTFANSMKTWAYRQDQLPSTHPIMAEIRDLDDVLVNFDGITYAKGASVLKQLVAY
                     VGRDEFFRGVQAYFKAHAFGNTQLSDLLGALEETSGRDLKIWSKAWLETAGINVLRPEI
                     ETDANGVITSFAVRQEAPALPEGAKGAPTLRPHRIAIGLYDLDDASGKLLRDERIELDV
                     EGPLTAVPQLVGQKRPAVVLLNDDDLSYAKVRLDEESLRQVTEHLGDFEASLPRALCWA
                     SAWDMTRDGELAARDYLSLVLSGIGKESDIGVVQSLHRQVKLALDLYAAPDFRAEGLAR
                     WTEAAHERLLAAEPGSDHQLAWARAFAATARTESQLDLLSDLLDDRATIEGLAVDTELR
                     WTFVLRLAATGRIGEKEIAAEYERDRTAAGERHAASARAARPTAEAKAEAWASVVDSDK
                     LPNAVQEAVIGGFVQTDQRELLAPYAEKYFAAVKGVWESRSHEMAQQIAVGLYPSLQVS
                     QHTLDATDAWLDAAAPNAALRRLASESRSGVERALRAQAVDAAA"
     gene            complement(18470..18970)
                     /locus_tag="GCN81_RS14855"
     CDS             complement(18470..18970)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103163.1"
                     /locus_tag="GCN81_RS14855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1203 domain-containing protein"
                     /protein_id="WP_156725082.1"
                     /transl_table=11
                     /translation="MTTTYHARPIAPAALQQLRDTDDAGRPCAPYTDGEGGAPLRCCLR
                     LSAAGERIALVSYAPLRRWAAERWARPGAYDEQGPVFIHAEDCGGPAGRERYPFDRAGA
                     LRVLRRYDSEGRIVGGRLLEIPEDAASGFDAAFAEAFADPAVALVHVRAVTYGCFHFEV
                     RRP"
     gene            complement(19082..20110)
                     /locus_tag="GCN81_RS14860"
     CDS             complement(19082..20110)
                     /EC_number="1.2.1.11"
                     /GO_function="GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA]"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948068.1"
                     /locus_tag="GCN81_RS14860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_156725083.1"
                     /transl_table=11
                     /translation="MRVGIVGATGQVGTVMRGILAERKFPVDELRLFASARSAGKTLDW
                     QGQDITIEDAATADYSGLDIVLFSAGGATSKALAEKVASQGPVVIDNSSAWRRHPEVPL
                     VVSEVNPHAVANRPKGIIANPNCTTMAAMPVLKPLHLEAGLTSLVVATYQAVSGSGLSG
                     VQELNDQIQKVGDDAPKLTHGGDAVEFPAPDNYVAPIAYNVLPLAGSIVDDGLNETDEE
                     QKLRHESRKILEIPELKVSGTCVRVPVFTGHSLQVNARFERPLSPERATEILSGAEGVV
                     VTDVPTPLVAAGKDDSFVGRIRNDETAENGLAFFVSDDNLRKGAALNAVQIAELVAAEL
                     KG"
     misc_feature    20265..20279
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
ORIGIN
        1 agcgtgcgcc agccctcgcc cggcacatgg gcctcgacgg agcccgccac gccgggcgcg
       61 gtcagcgcgg tgatcgcgtc caggcggcgc gggcggggga tacgtacggt gtacgcggag
      121 tcgtcccgct cgggcttcgc cagctggcgg ttcgcgccgg tccaggccgc ggagtcctgc
      181 tggacgcggt cgaggaacgg gtcgagtacg cccttgccga ctgtggcccg gccggtcttc
      241 accgcctcgc gcgccgcctt cagcccgcgc tcggcggtcc aggcggccgc gccgtcgccg
      301 cgtgactgcg cgtgcagcag gtccacggcg tactcgcccg cggtgccgta gcgggccaac
      361 tgcttcagcc agggcccggt ttcggcgccg agcgggctgc ccgcgaggtg ctcaggggct
      421 tgccgcaggg tggtgaaggc gccgcgcagc ttcttggccg tgcgggcgat acggtccagg
      481 tccgcgctgc cgcgtgcggt gcggaactcc tcgatcagcg ggcggagata cgccgactcc
      541 tcgtcgctga ggatagacga ggcggtgttg cccgccagcg cgcggacagc ctcgcggatc
      601 gcgccggcgt ccgcctcggt gtccttcgac tcccgtgtcg tctcgcgcgt cgtcgtgtca
      661 gccaggtcgt cgatcgccgc ccgccaggac tcccgcgggc ggtactgctt ggggttccag
      721 gcgtagtcgg cggccgtgaa cagcgggatg cgggaggccg cggcctgctc catcgcgttg
      781 gacagcaccg ccaccgaacc cgcggccacg gcgggctccc ggccctggta ggggcccagg
      841 aagatccggt cctgtgcgta gtcgttgacc gagtagttgt ccatggtgac cagcggatgc
      901 ttgaacgcct cgcgggccac ggccagttcg cgccccgtga tggtcttcgg caccacgccg
      961 acgcccgtcc aggcgatatg caccccgccg tccaactccc gcgccagcgc cgtgcggtac
     1021 tcggtcgcgc cttgctggta gtgctccgtc ggcagcagcg acagcgcggc cgcgtcgccg
     1081 taccgctccg ccaggtgcgc cgtcaccgcg cccgcgacgc tggcctgggc ggcggcggcc
     1141 gcgtcaggac ccgagccgaa cttgtcgcgg tcggctcggc agtgccactc gctgtacgag
     1201 acgtcctgga actggagttg gaaggcgcgt acgcccagcg cccacatggc gtcgatcttc
     1261 cgcttcagcg ccttgaggtc ggtgtcggac gacaggcaca tcgcctgacc cggcgccacc
     1321 gcccaggcga gcgtcacatg attgcgccgc gcccgctcgg ccagcgcccg gaactcggcg
     1381 cgccgctcgg ccgggtacgg gtcgcgccag cgggcctgcc ggtacgggtc gtcgccgggg
     1441 gcgtacagat agcggttctg cttcgtacgt cccatgaagt cgagctgagc caggcgctgt
     1501 tcacgggtcc atggggtgcc gtagaagccc tcggtcatgc cgcgtacggc ggcggtgggc
     1561 cagtcgcgga tcgtcacgcc cggcacggag cggtcggtga tcagctggcg cagcgtctgt
     1621 acggcgtgga acaggccgtc gtcgccgaca ccggccagga cgaccgtgtc gcggccttcg
     1681 acccggccca cggcgacgcg gtagccgcca ggcggcagat ccgcgcgctc cggggccttc
     1741 agggcgcgca gcgcgctctc cgagccggag cggccctcgg gtcccgtacg ggcgggctgg
     1801 cccccggggc cgcccggggc ggcgcgggtc gcgcggctgg cccgctcgtc gcccacgcgg
     1861 atcaccaggc cacgggcggg caggcgggcg ggcagcgcgt cggcgtcgga cagctcggtg
     1921 accttccggg cacctgctgt cgccaggagg gtacggagcg tgtcgatcgc gtacgggtcg
     1981 gcgccctcat cggcgagcag gaacacctcg ccgtcgatcg ggaccgcggt tccggacgcc
     2041 tggatcgact gtggtttcgg ccacactgag gggagtttcc cggctgtttt ccggcccgtt
     2101 tcgcggcctg ccgtgtccgc cgcgtcggtg ctcgggttta ccgggtccga cggggccgcg
     2161 agcgcgccgg gggcgctgcc gagcaggccg ccgatgaccg cggccacgag cgctgccgcc
     2221 cctcttccgc accggagctg cacgggttcc ccgcccttct gcgtcagtcc ccgtcgtgag
     2281 gttccaggga gcagctgagc ccaccatcgc ggtggtgagg gtgtcaacgt gggtgaccgt
     2341 tgtgtcacgt ttgatcatga gtgtcgcggg ttggtgaggg tgaacgggtg tggctgggga
     2401 ataactaccg caggccgggc ggcgtgtgag cagcgccaca tggcccgaat ggtgagcgat
     2461 ccagtgggta agggcccgca tggaacccgc caccaccaca cccgaggagc cctccgtggc
     2521 caccaccact taccttgtgc gcgacgccta ctccaaacgc caggagacgc gcgacctgcc
     2581 tgacctgtcg ctcgtctccg gccccgactc cggactgctc ggctcctgct cctgcggatg
     2641 cctggagcca ccgttgacca gcgagccgcc gctggccgac gccgccccct tgaccagtga
     2701 gccgccgctg gccgacgccg cgccgctcac cagcgagccc accgccaccg gtaccgcgtc
     2761 ctttctggac ccctcggcgt actagatgcc tcgaccccgc gagcgggagc ccggtgctga
     2821 gccccttgac ctgctggccg cgcggtacgg cgtggccacc tcctacgagc cgtcccctgg
     2881 ccggacggta cgggccgaac ccgcggcggt cgtcgccgtg ctcgccgcgt tgggcgtgga
     2941 cgcgagcaca cccgacgcga tacgcgccgc gctgaccgca cgcgaagccg aactccgcga
     3001 gcggctcctg ccgccgactc tggtggcctg ggcgccggtt gggccggttg ggcccgttgc
     3061 gtcgggtgag ccggttgctc cggttgagcc gtcgcccgag ctggtctcgc ctccgcttgg
     3121 cgcgctggag aagcttgccg ggctgccgac gggcacccgg ctgtgggtgc gtaccgagca
     3181 gggggacgcg gccggttggg tggtcccgga ggcatctggg ggctgtgggg cgccagggac
     3241 gtctgaggcg gctggggtgt ccggcgcctc cggtgacggc gtcggcgggc cgcggctgcc
     3301 gttaggggtg cacgaggtgg gggcggaggc ccccgacggg cggcgcgctc aggcatacct
     3361 cgtcgtcgcg cccgagcgga tgcccgcgcc gggagagcgc gcgcacgggc tgctcgtgca
     3421 gctgtactcg ctgctctcgc ggcgttcctg gggcatgggt gacctcggcg acctcgccga
     3481 tctcgccgcc tggtcggggc gggcgctcgg caccggcttc gtccaggtca acccgttaca
     3541 cgcgagcgtc cccgggggcc ccggcgggcc gaccgacccc tcgccgtacc gcccctcatc
     3601 gcgccgcttc cccgaccccg tacatctgcg cgtcgaggac atccccgagt acgtctatct
     3661 ggcacccgag gcccgccagg aggccgccga gctgctcgcg cgggccgcca tcctgcgtca
     3721 aggcgtgctg aacgagggcg cgttgatcga ccgcgacgcg gtgtgggcgc tcaagtcccg
     3781 ggccctggag ctgctacgcg aggtcccgct gagccccggc cgacgcgccg cctactgtga
     3841 cttcctcgcc gagcagggcg agcccctgga ggcctacgcc acctggtgcg cactcgccga
     3901 gcggtacggc tccgactggc cctcctggcc gtcgcccctg cgcgatcccg catctcccgg
     3961 caccgcccgc gcccgggccg ggctgatgga gcggatcgac ttccactgct ggctcggctg
     4021 gctcaccgac gcccagctcg ccgccgccca acgcgccgcg ctcgacgccg ggatggcgat
     4081 cgggctcgtc cacgacctcg cagtaggcgt ccatcccggc ggcgcggacg cgtgggcgcg
     4141 gcgggagttc ttcgccgcgg ggatgtcggt gggcgccccg cccgacgcgt tcagcgcgga
     4201 cggtcaggac tgggggctgc cgccctggcg tccggacgcg ctggccgcct ccggctacgc
     4261 ccccttccgc gacctgctac gcgggctgct acggcacgcg ggagcgctgc gtatcgacca
     4321 tgtgatgggc ctgttccggc tctggtgggt gcccgccggc cgcccgccga ccgagggtgc
     4381 ctatgttcac tacgacgccg acgcgatgct cgccgtcctc gccctggagg cccaccgcgc
     4441 cggggccgcc gtcatcggcg aggacctcgg caccgtggcg ccgggcgtcc gcgaggcgct
     4501 ggccgcccgg ggcgtcctgg gcacgtcggt gctgtggttc gagcgcgact gggacggtac
     4561 ggggcggccg ctgcccccag accgctggcg cgccgactgc ctggccaccg tcaccaccca
     4621 cgacctgccg cccaccgccg cccgcctcag cggtgaacac atcaggctgc accagcggct
     4681 cggcctgctg accgggccgc tgtcgcgggc cacggccgag ggcgcggcgg agacggggga
     4741 gtggctggcg gtgctggcca ggctgggact gctgccgggc ggccgcggtg cgcggggcga
     4801 ggaggagaag atccgggccg tctaccgctt cctggcgcgc accccggccc ggatggtcgg
     4861 cgtctggtta ccggacgcgg tcggcgaccg ccgcccgcag aacgtccccg gcacctggcg
     4921 tacgtacccg aactggcgcc tccccatcgc cgacgccacc ggccgcgcgg tcaccctgga
     4981 ggaactcgcc gcctcgcccc ggctgcaccg gctgatggcg gagctacgcg gtctctgaga
     5041 gccccgaacg gcaccccggg cgcgcggcgc gtgggggcgt tcgctacgtt ggcactgtgg
     5101 acaagaagaa tgccctgcgc gccggcgccg tagcagccgg tacgacgctg atgatgctgc
     5161 tcatgacgtc ccccgcgctc gcgctgaccc gcgacgacgg cgacgacccg gctcccaagc
     5221 tgagcgtgat ggagaccctc ggcctgtacg tcgcggctcc gatcgccctc ttcctgctga
     5281 tcgcgggcct ggtgatggtc ggcgacaagt cgaaggaccg gcagccgaag ccgcagaagc
     5341 agggctgacg gcccggccca cacccgcacg tcgcgccgag ggcgcccctc ctgagtgagg
     5401 ggcgccctcg gcgcgtttcg cgttccttgt tccctccgat tgttcagttc aactgaaaag
     5461 aacggtcaga agatttcgat ggacctaaca ggacggagcc tgcgacggta gccgcacacc
     5521 acctcaccac cacagccccc agccggggga gagagcaggg ggatactccg tggcgatcct
     5581 ggaccacgtc cgcggcagcg agccgaccga gcgcgtacgg cggcggtcca cggggccgag
     5641 gccagggaag cgccgggccg cccaggcggc ctccgccgcc tccgcctccg gcctcggcct
     5701 cgtcctcgcg gccctcgcca cggtcgtctg gtccggcagc ttcgtcacct cccgcgccct
     5761 cgccgacacc gtcccacccg tccagcacgc cttctggcgc tggatcatcg ccctgatcgc
     5821 cgtcgccccc ttcgcgaccc gcgcggcctg gcgtcagcgg gacctgatcc gccgccacat
     5881 cggatacctg acccttgcgg cgttcctcgg cgtcgccacg tacaacaccc tggtcaacca
     5941 ggccgggatc tccacgtccg ccggaaacat ggccatgatc atggccgcgt ccccggtcct
     6001 gatggccctg tatgagcgcc tcggtggcac ccggctcggc gcccgccgcg ccctcggcat
     6061 gctcatcgcc tgcgccgggg tgctgctgct ggtcagcaag ggctctctgg cgatggactt
     6121 caccaccggc gacctgtgga tgctggccgc cgcgctgtcc ttcgcctcct acagcgccct
     6181 gctgcgcggc aggcccgccg ggctcggcgg cctcgccttc ctgttcacca ccttcgcgct
     6241 cggcgccgtg atgctcgccc cctgctacgg cgtgagcctt gccgtccagg gtggcttcga
     6301 ggtcaccacg ggcacggtcg gcccgctgct gtacgtgggc gtggtctcgt cggccgtcgc
     6361 cttcttcgcc tggaacaagg cgatcacgct catcggcgcg gcccgcgcgg gcgtcatcta
     6421 ctacctacag ccggtgtgcg tcgcggccct gtccttcgcg atcctcggcg agtcggtcgg
     6481 cgtgctccag ggggcctgca tggcactgat cctgggcggc gtgatcctgg cctcggcccg
     6541 cgcccggtga gctgggccca ggcctgtggg gttcccacag atctgggcag gggaggctgg
     6601 ccgaaggctg tcgcgtctga acggatgggg tgggtgagga taggtgggtg cctgatctcg
     6661 tggaccccgc tgccctggac cccgctgccc tggactccgc tttgctggac cccgcgtcct
     6721 tggcccccgc ccccgccccc gcccccgctc tcctggaccc ggccgacgct gacttcgccc
     6781 gcgacccgta tccgtactac gcacggctgc gcgcccaggg cccggcggta cggactccgc
     6841 tgtccaacgg gacccacgcc tggctggtga ccggctacca ggccgcgcgc gcggtgctgg
     6901 ccgacccccg gttctccaac gtcccggcgc ctcgcgcggg gcggcccaag cccgactcgg
     6961 cggccgagcg ggcccgggcc tgcctggcgc ggcacatgct caacctcgac gcccccgacc
     7021 acaaccggct gcggcggctg gtgacgggag cgttcacgcc gcgccgggtg gacgcgttgc
     7081 gcccgcggat cgagggtctt gtcgggccgc tggtggcgga cgtacgggcg cggcttgacc
     7141 gggacggtgt cgccgacctg gtggacacgt tcgccttccc gctgccggtg ctggtgatcg
     7201 ccgagctgct tggcataccc gaggcggacc gaacgagcct gcgggagtgg acgtaccggg
     7261 tcggatcgcc cgccgactcc ctcgcgcccg gtgaggtgga ggaggcgtgg gggagtctgc
     7321 acggctactt caccgagctg atcgcccaga agcggcgctc cccgggcgat gacctgttca
     7381 gcgtgctggt gcacgaggag cgggaagggg cacttgatga cggcgagttg ctggcgctgg
     7441 cgttcctgct gctcttcgcg ggctacgaga cgaccatgaa cctgctgtcg tccgccgccc
     7501 tgctgctgat cacccaccgt gacgagctcg ccgccgcgcg gcgcgagggt acgcggcgct
     7561 gggacgccgt cgtcgaggag acgctgcggc acgccagccc gctggagggc gcgacctggc
     7621 ggcgtacgac ggaggaggtc gacctcggcg cgggggcgct catcccggcc ggggagtcgg
     7681 tcctggtggt gctggccgcc gccagccgtg accccgcgtc cttcgacgcg ccggacgact
     7741 tcaggcctgc gcgctatctg agtggtgcgg gccgggcggc gggacatatg gcgttcgggc
     7801 atggcgcgca tgtgtgcctg ggcgcgcgcc tggcgcggct ggaggcgggc gtcgcgctgc
     7861 cgatgctgtt cgacgcgctg ccgaagctcg gcctcgcgga tgacgtcgcc gaggttccgt
     7921 accgcccggg tctgctcgta cggggcccac gggtcctccg cgtcaggacc tgaacggaca
     7981 gcggaacggg ataggttgcg gcgcgtgagc gagtgggaca tcaagaagct gcggatcctg
     8041 cgcacgttga gcgagcgggg gaccgtgacc gcgacggccc aggagctgct gatgacaccg
     8101 tccgcggtgt cccagcagct cagcagcctc gcccggcagc tcggcgtccc gctgctcgaa
     8161 gcacgggggc ggcgggtccg gctgacggac gccgcccatc tcgtactccg gcacgcggag
     8221 gtcgtgttcg cccaactgga gcgggccgac ggggagttgg ccgcgtacgc acgcggagag
     8281 gccggggagg tgcgggtcgg cgcgttctcc acggcggtgc ccgccctggt cgtcccggcc
     8341 gtacgccacc tgcgtaccga agcgcccgcc ctggaggtac gggtccgcga ggccgaggcc
     8401 gcccaggcgt acgaactcct cgcgggcggc gccgtggacc tggccctgtc gctggccgcg
     8461 cacgcgccca ccgcgcggga ccccaagttc acccgcgtcc cgctgctcgc cgaccccctg
     8521 gatgtcgcgc tcccggtgga ccacccactg gccggggcgg agtcggtacg gctcgccgat
     8581 ctcgccgaag agcagtggat cttcggcggc agcggcccct ggtccgagat caccaccacc
     8641 gcctgcgaag cggccggttt cgtccccgaa caggcccaca gcgcggccgg ctggaccgcg
     8701 atcctggcca tggtggaagc cggaatgggc gtcgccctgg tcccccgcat ggccgtcgcc
     8761 ggacgccagt cgggcgtcac ggtacgcgtc ctgaccggcg accgcccccg ccgccacgta
     8821 gtagccgccg tacgccgagg cgcggaccag gcccccaccg tggcccgcgt cctgaccgcc
     8881 cttcaggcgg cggccccggg ccaccacgaa gtgaacgccc gcagtgccac gggctgggga
     8941 acagggcacg gcctcccgcc ccgggtgagg aaaacaactg ggtgagtgtt ccggggatct
     9001 cacggtatgt gagcttagag agggtgccgc aaacgatcat tttcgggcag ggttgatccg
     9061 ctcgtgcccc ccacaaccgt tcaccggaga cttgatgaac cgcagcctca cccccttcga
     9121 cgagtcccaa gtccccctct actactcacg caagaccgcg gacatcctct acaagtacgg
     9181 cccagggccc cgcgtccact accacatggg cctcttcgag ccgggtgcgg cacccaacac
     9241 caccgtctcc caacactccc tgaagcgccg gatcgtggcg tcccaggagg ccatcgtcga
     9301 acacgcggcg cgtagttggg gtgcccacca ggccgctccg ggctcggtcc tggacatagg
     9361 ctgcggtctc ggcggcggct cgatctactg ggcgcaggag cacggagcca ccgtcaccgg
     9421 cctcaccgtc gcggccgagc acctccccgt catcgcgggc ttcgcccgcc aggcgggagt
     9481 cgccgggcag gtcaccccgc tgctcgccga cgtccaccag tacaccgcag accggctgtt
     9541 cgacgccgcg tacgccaacg agtcctccgg gtacatggac cgcgagcggc tctttcaggt
     9601 cgtcgccaag tcgctgaagc cgggcggctg gttcggcatc caggagcact tcatctgccg
     9661 tccggagtgg accgagttca tcgacggcta ctacaagacc cgactgggca cgctgaccga
     9721 gtacatcacc gccgccgagg ccgcgggctt cgagctggag caggacgagg acgtcaccga
     9781 ccgggtcgcc gagttctggg tgcagtccat ggcttggaac accgctgaac tggagcgcgt
     9841 cgagcgcggc ggcgcccctg agcgcctcgc ctggagccgc gagcggctca ccgagtcggc
     9901 gctgacccac ggcaagctgt tccgcatctg gcgcgaccac gcactggaga cccggctgct
     9961 gatgttccgc ctcgcgggcg ggcccggagg ccgctgaatc atgcctcccg tgtacgccgc
    10021 cgataccgcc ccgtcccgca tcggctggga actcccgccg ttctactgtc cgttcaacga
    10081 gcaactgatc caccccaagg cggcggagct cgaaacccgc gccatcgagt ggatcgacgc
    10141 cttcggcatc tactccgacg cgaccgagcg ggcctggggt ctggccaccc acagcaccga
    10201 cttcacctcg cggatcatcc cgtacggcga cgtggaaccg atcctgctct tcgtcgagtg
    10261 gaactactgg gccaacgcgg tcgacgactg gcaggactcc ggagacaccg ctaccaggac
    10321 ggccgccatc gccgaccaca gcgtacggct ggtccgctcc atcgaggcac ccggctcgtc
    10381 cctgttgccg ccgggcccgc tcaccaccgc gctggacgac ctggtcgccc gcacccgggc
    10441 catgctcacg ccctcccagc tgcgccgttt cacggagggc acccgggact ggcttttcgg
    10501 cgcgggctgg cagaccgcca acgccgaact cgatgtgatg ccctcgctca acgacttctg
    10561 cgccatgcgg atgtcggtca acggtacgcg cttcacgctg acctggtgcg atgccgcgaa
    10621 cggcattcac gtaccggccg atgtgctgta ctcggcaccc gtccaggcgc tcaccgacgc
    10681 cgccggcttc atcgtgagct gcgacaacga cctgttctcc tacaacaagg aggaccacct
    10741 gaacccctgg gagcagaacc tgctcaacgt cgtcgcgcag caccaggggt gtaccccggc
    10801 agaggcgatg ccagccgcgg tggccctacg ggaccgggcg atgacgctct tcgtacggct
    10861 gcgggagcag ctgtacgccg gggcagggga cgatctgcgg cgctatctgg actcgctggg
    10921 gcactacgtg tccgggtgca tcgaatggca gaacctggcg ccgcgctata ccagcccgcg
    10981 taaccgtaac gcgctccccg tggaaggggc ttcgtacgac gtgacgtggc gcgacacacc
    11041 gagcgacccc gagcaggggc cgccgccgat tccggcgatc gcgtggtggt gggcccagct
    11101 gcggtgacct tcgccccgcg ggacgggctg aaagaccagc ccgtcccgcg ggcgacaggt
    11161 caccgcctgg cgaacagccg cgcccaccac gagcgccgca ccggctccgg cgcgagggcc
    11221 gcgggcggtg gcgccgcggg cgggggcgcc gcggcagccg ggaggggctc cgccaccggc
    11281 gggcgaggca ggggcgtggc gccggtacgg ctgcccagtg gtgtgaaggt gaccggcagc
    11341 gccaccagac ggcgcgacat cagcgtcgag tcatagcgca actcgtcctc cggcaccccg
    11401 agccgcagat ctggcagtcg cgacgtcagc gcgtcaatcc cggtgtccgc gatcgcccgc
    11461 ccgacatcct gtccgggaca ttcgtgcggc cccccgctga aggcgaggtg ggagcggttg
    11521 ccgtgcaccg ggctggtcag gtcgggccgg atctccgggt cggtgttgcc tgcggccagg
    11581 ccgagcagca gcatgtcccc ggccttgatc cgctgcccgc cgagcatggt gtctccggtc
    11641 gcccagcggc ccaggatcgt ggtgagcggt ggttcgtccc acagcacctg ctccagcgcg
    11701 tcgggcagcg tcatatgccc gccggacagg tgcgcccgga agcggcggtc ggtgagcatc
    11761 atgcgcaggg tgttcgcgat caggttggcg gtcgtctcga aggccgcgat caccacgagc
    11821 cgcaggtgct cgatgatctc cgcatcggtc agcgacgcgc tgtgctcaag gagccaggac
    11881 gtgaagtcgt ggccgggcga agctcgtttg gcctccaaca gctggcgcag cgcgaccgag
    11941 agatactcgt tactggcgat ggccgtgtca gtgccccggg tcatgtcacg cgcggcctgc
    12001 accaggcgcg gaccgtagct gtcgggcgcg ccgaccaact gggtcatcac cagcatcggc
    12061 agcggctcgg cgaagtcggc gaccaggtcg gcccggcctg cctcggcgaa cttgtcgatc
    12121 agctgaccgg cgaagcgcgt cacatagcgg cggatgcccc gccggtcgaa ccgctccagg
    12181 gactcggtga ccgccccgcg cagccgctcg tggtcggtgc cgtccgcgaa gttgcacacc
    12241 ggctgccagg ccgcgacagg ggtcagcgga tggtcggccg gtacgttccc ctcctgcata
    12301 tcgcgccagc ggcgggagtc ccgcgagaag cgcgaggggg tacgggccac gtccaggttc
    12361 tcgcggtgcc cgagcaccag ccacgcgggc aggtccccct gcaccagcac gggggccacc
    12421 gtgccgtgtt cggcgcgcag cgcttcgtac aggccgcgcg gatcggcctc ggcctcgggc
    12481 ccgtacagcc tgcgcagtcc ctcggggctc atggcgtgag ccgggcaccc cggcggcggc
    12541 gtcccgccgg acggcaccgg ggtggcgggt tcggggctgg tcacgaggcc tccgtggtcg
    12601 cggcgaggga ctgcaggtag cgcatcaggg ccatcaggac gtcccggctc gaagcgcgtg
    12661 agcgggcgtc gcaggcgacg atcggtaccg acgccggcag gtcgagcgcg ccgcgcagtt
    12721 cctcgatggg gtgctcgggg gcgtccggga aggagttgac ggcgaccacg aacggcacgc
    12781 cgcgttcctc caggcgcccg atgacgtcga agctgacttc gaggcggcgg gtgtcgacca
    12841 gcaccaccgc gccgagcgcg ccctcgaaca ggccgcgcca caggaaccag aagcgctcct
    12901 ggcccggagt gccgaacagg tacaggacca gctcgtcgtt gatgctgatc cggccgaagt
    12961 ccatcgccac ggtggtggtg gacttacgct ccacacccgc cgtgtcgtcg acgccgaccc
    13021 cggcctgggt catggtctcc tcggtggtca gcggcctgat ctcgctgacc gacccgacca
    13081 gtgtggtctt gccgacgccg aagccgccga cgatcaccac cttgaccgcg gcggtggccg
    13141 tctccggcag cacatcctca ctccgcggcc ccacggtcgg ctcagagctt ctgaagtcca
    13201 tcgatcactg cctcaatcaa cgcgatgtcg gggagtttgg cgggcggtac gggcggccgc
    13261 gaggtcaccc gctggtcggc gagcagatca ctgagcagta cggtcaccac gctcaccggc
    13321 agccccaggt acgcggagat ctcggcgacc gaaagcggcc gctggcacag ccgtacgatc
    13381 gccgcctgct ccggctgcat ccccgggccc ggacctgatg tggccacgat cagggtgacc
    13441 aggtcgagcg cggtcggggc cgacgagccg ctgcggcccc cggtgatcac atagagccgt
    13501 tccgggccgc cctcgtccca ggcctggccc gggccaggct gggtcacctg gcggcctgcc
    13561 cgtcctgccg gggcgggctg ctcaggtgct ctccgatccg cagtacgagg tcccgcatcc
    13621 gctgacccat cagtcccgcg tccacaccct cgtcggccag gacggcgagg taggcaccgg
    13681 cgcccgccgc catcagatag aagaagccgc cgttcatctc gatgacgaca agccgcatct
    13741 ggccgtcgct gtccgggagt tcggccgcga ccgcgcccgc gaggctctgt agccccgcgc
    13801 aggcggcggc cagccggtcc gctgagtcgg tgtccgcgcc gaactgggcc atccgcaggc
    13861 cgtcggcgga gagcacgacc acctgccggg tctgtggcac gccctctgcc aggtccttga
    13921 gcatccagtc catgttggtg tggctggcgt ggctccccgg gtggccctgc tgctgcgtca
    13981 ctgctcgttc cccttgctag ctgacgcctc ggaggcgtcg tggtcctggc cggctggacc
    14041 ggcctgtgtg gtgtgctccg ggtcggtcac ggcctgagca ctctcgccgg aaagcccgct
    14101 ctggaaggcg gccagccata tgccgggctg taccggcggg gcgggctccg acggggcgga
    14161 ctccggcggg gtcgcggctg cggccgacgt ggccgcggcc gacgcggtgg gtgtggccgc
    14221 ggtcggcctg cggcggcgct gcggcaggcc gttcgccgtg cgttcggcca ccacgggagc
    14281 gtcgtcggtc accggcgcgg gcccggtcac cgggcggggc gccgtcccag ccgccgtacg
    14341 cgccgccgta cgcgccgtcc cggccgccgc gcgcgacggc tcggcggagg cacgcgggcc
    14401 cgacgcggtg ccgatcccgt gcgcgaggcc ggtcgcctcg gccgccgtgg tgatcaggtc
    14461 ctgcgggacg accaggacgg cccgcacacc gccgtacgcc gaagggcgca gcgagacctg
    14521 gaacccgtac acctgcgaga gccgcccgac cacggcgagg ccgagccgtg gggtctcgcc
    14581 caggtcgttg acgtcgatgc cctgctgagc ctgcttcagc atccgctcag cccggccgcg
    14641 ggcctcctcg ctcatcgaga cgccgccgtc ctcgatctcg atggcgatgc ccgactgcac
    14701 gtcgacggcg gtcagatgga cccgggtctg cggcggcgag tagcgggtcg cgttgtccag
    14761 cagctcggcc agcgcgtgga tcagcggctc gacggcggtg ccgacgacgg cgacctcgga
    14821 gaccgagtgc agctcgacgc gctggtagtc gatgatccgc gacatggagc cgcgcagcac
    14881 cgagtacagc ggcaccgccc gggaccactg acgccccggc cgggcgccac cgagtacggc
    14941 gatggagtcg gcgagccgtc cgatcagcgc ggtgccgtgg tcgatccgca gcaggtcgcc
    15001 gaagacctcg gggtgtccac cgtgacggtc ctccatctcc cggagttcct gagcctgttg
    15061 gtgcacgatg gcctggacgc ggcgggcgat gttgacgaag gcgcgctgtg cggagtcgcg
    15121 caggtcctct tcggcggtga ccgcgtcgac gaccgagcgc agcaccgcga ggtgcgcggc
    15181 gggggccgac agcgagtcga gtacgtcctc ggggaactcg cccttgcgca gccgcgccac
    15241 gacgtcgggc agcagctcct cggccagccg tacggtctcc gcctcccggc gggccagcgc
    15301 ctcgtcccgc tcggcggccg tggcccgcag ctcggcgatc tcccggccgc ggcgggccgc
    15361 gatggcgcag gcgacggcga gcccgacgac ggcggcccca cccgtgacga gtacggccgc
    15421 ccgccccgag tcgagcaacg ccgcggccag cgcggtcagg gcggcggcag cggcgggcgg
    15481 cagaagccag agcgggaagg gggacttggg acttcctggg gatgtccccg tgcgaaccct
    15541 ctgcactgca gctcctgtac gtaccgaaga tcaccggaca gctcggtagc gtagcgcgtt
    15601 tggagtaacg gcagggagtt gggtgtgtgt tgaccgcgta aatacggtat ggcgtcgcgc
    15661 acggcgtacg gcgcgcggtc atcgcggtag ggcgcccgcc cggcatcggg gccctcaaca
    15721 agcggagggt cccgatgccg gacagacagc cggacagaca gccggacaga cagacaggcg
    15781 gcccttagac agcccttaga cggtcctagg ctgcgctgcc cctaggccgc cgcgtccacc
    15841 gcctgggccc gcagcgcgcg ctccacgccc gagcgggact ccgaagccag ccgccgcagc
    15901 gccgcgttcg gcgcggccgc gtccagccag gcgtccgtgg cgtccagcgt gtgctgggag
    15961 acctggagcg aggggtagag cccgaccgcg atctgctggg ccatctcgtg cgagcgcgac
    16021 tcccagacgc ccttgaccgc agcgaagtac ttctccgcgt acggggcgag gagttcacgc
    16081 tggtcggtct ggacgaagcc gccgatcacc gcctcctgaa cggcgttggg cagcttgtcg
    16141 ctgtcgacga ccgaggccca ggcctccgcc ttcgcctcgg cggtcggccg ggccgcccgc
    16201 gcgctggccg cgtgccgctc gcccgcggcg gtgcggtcgc gctcgtactc ggccgcgatc
    16261 tccttctcgc cgatcctccc ggtcgccgcg agccgcagca cgaacgtcca gcgcagctcg
    16321 gtgtcgacgg ccaggccctc gatcgtcgcc cggtcgtcca gcagatccga cagcaggtcg
    16381 agctgggatt ccgtacgggc cgtcgcggcg aacgcccggg cccaggccag ctggtggtca
    16441 ctgccaggct cggcggccag cagtcgctcg tgcgcggcct cggtccagcg ggcgagaccc
    16501 tcggcgcgga agtccggcgc cgcgtacagg tccagggcca gcttcacctg ccggtgcagc
    16561 gactgcacca caccgatgtc cgactccttg ccgatgccgg agagcaccag cgacaggtag
    16621 tcgcgggccg ccagctcgcc gtcgcgggtc atgtcccagg ccgacgccca gcacagggca
    16681 cgcggcagcg acgcctcgaa gtcccccagg tgctcggtga cctgacgcag cgactcctcg
    16741 tcgagccgga ccttggcgta cgacaggtcg tcgtcgttga gcagcaccac ggccgggcgc
    16801 ttctggccga ccagctgcgg tacggcggta agcgggcctt ccacgtccag ctcgatccgc
    16861 tcgtcacgca gcagcttgcc ggacgcgtcg tccaggtcgt acaggccgat cgcgatgcgg
    16921 tgcggacgca gcgtgggcgc gcccttggcg ccctcgggca gcgcgggcgc ctcctgccgt
    16981 acggcgaagg aggtgatgac gccgttggcg tccgtctcga tctccgggcg caggacattg
    17041 atacccgcgg tctccagcca cgccttcgac cagatcttca gatcgcggcc gctggtctcc
    17101 tccagcgcgc ccagcaggtc ggacagctgg gtgttaccga aggcgtgcgc cttgaagtag
    17161 gcctgcacgc cccggaagaa ctcgtccctc ccgacatacg ccaccagctg cttcagcacc
    17221 gaggcgccct tggcgtacgt gatcccgtcg aagttgacga gaacatcgtc gaggtcgcgg
    17281 atctcggcca tgatcgggtg cgtggacggc agttggtcct gccggtacgc ccaggtcttc
    17341 atcgagttgg cgaacgtggt ccaggagtgc ggccacttgg agtccggcgc gtacgcctgg
    17401 caggcgatcg aggtgtacgt cgcgaacgac tcgttcagcc acaggtcgtt ccaccactcc
    17461 atggtgacca ggtcgccgaa ccacatgtgc gccagctcgt gcaggatggt ctcggcgcgc
    17521 atctcgtacg ccgcgtccgt caccttcgag cggaagacgt actggtcgcg gatggtgacc
    17581 gcgcccgcgt tctccatggc gcccgcgttg aactcgggca cgaagagctg gtcgtacttc
    17641 tcgaaggggt acgcgtagtc gaacttctcc tggaaccagt cgaagccctg ccgggtgacc
    17701 tcgaagatcg cgtccgcgtc caggtactcg gcgagcgagg ggcggcagta gatacccagc
    17761 ggcacgctgc ggcccgggcc gtcgtaggtg ctgtgcaccg agtggtacgg accgacgatc
    17821 agcgccgtga tgtacgtcga gatccgcggg gtcggcgcga agacccagac gtcgtccttc
    17881 ggctcaggcg tcggggagtt cgagatgacc gtccagccgg agggcgcctt cacggtgaac
    17941 tggaacgtcg ccttcaggtc cggctgctcg aacgacgcga agacccgccg ggcgtccggc
    18001 acctcgaact gggtgtacag atacgcctgg tcgtcgaccg ggtcgacgaa gcggtgcagg
    18061 ccctcaccgg tgttggtgta cgcgcagtcc gcgacgacct tcagctcgtt ggggccctgg
    18121 agcaggttcg ccagggtgat ccgggagtcc gcgaagacgg cggcggtgtc gagcgcgtcg
    18181 ccgttcagga ccacctggtg cacggtcggc gcgaccaggt cgatgaaggt ctcggccccg
    18241 gcttcggcgc tctcgaagcg cacggtggtc acggaccggt aggtgccgcc ctcctgcgcg
    18301 ccgctcagat cgaggtcgat ctcgtacgcg tcgacagtga gcagcttcgc gcgctgctgc
    18361 gcctcttcac gggtcagatt ggtgccgggc acgcggtcat ctcctcgggt tgcgatggtg
    18421 ctgaccatcc ttccacgagc gacgcgccgc gtccggggcg tttcgcccgc tacggcctgc
    18481 gcacctcgaa gtggaagcag ccgtacgtca cggccctgac gtggaccagc gccaccgccg
    18541 ggtccgcgaa ggcctccgcg aacgcggcgt cgaagccgga cgcggcgtcc tcggggatct
    18601 ccagcagccg ccccccgacg atgcgcccct cggagtcgta ccgccgcagt acccgcagcg
    18661 ccccggcccg gtcgaacgga taccgctccc ggcccgccgg acccccgcag tcctcggcgt
    18721 ggatgaagac gggcccctgc tcgtcgtacg ccccgggtct cgcccagcgc tcggccgccc
    18781 accgccgcag cggcgcgtac gacaccagcg cgatccgctc cccggccgcg ctcagccgca
    18841 gacagcaccg cagcggcgca ccaccctcgc cgtcggtgta cggggcacag ggccgcccag
    18901 cgtcgtcggt gtcacggagc tgctggaggg cggctggggc gatgggccgg gcgtgatagg
    18961 tcgtcgtcat gcgcccagcg tcgccgcacg cggccctggg caccggcggt ttccggacgt
    19021 ggaacccgcg aagggggcgg ccgccgtcac acggtggccg cccccttcgc gtacgggcgc
    19081 gtcagccctt gagctcggcc gcgaccagct ccgcgatctg tacggcgttc agcgccgcgc
    19141 ccttgcggag gttgtcgtcg gagacgaaga acgcgaggcc gttctcggcc gtctcgtcgt
    19201 tacggatgcg gcccacgaag gagtcgtcct tgcccgccgc caccagcggc gtcggcacgt
    19261 cggtgacgac gacgccctcc gcgccggaca ggatctcggt ggcccgctcg gggctcagcg
    19321 gccgctcgaa gcgggcgttg acctggaggg agtggccggt gaagaccggg acccggacgc
    19381 aggtgccgga gaccttgagc tccgggatct ccaggatctt gcgggactcg tggcggagct
    19441 tctgctcctc gtcggtctcg ttcaggccgt cgtccacgat ggagcccgcc agcggcagga
    19501 cgttgtacgc gatgggcgcg acgtagttgt cgggcgccgg gaactccacg gcgtccccgc
    19561 cgtgggtcag cttcggcgcg tcgtcgccga ccttctggat ctggtcgttg agctcctgga
    19621 cgccggacag gccggagccg gacacggcct ggtaggtcgc gacgaccagg gaggtgagac
    19681 cggcctccag gtgcagcggc ttcagcaccg gcatggcggc catcgtggtg cagttcgggt
    19741 tggcgatgat gcccttgggg cggttcgcca ccgcgtgcgg gttgacctcc gagacgacca
    19801 gcggcacctc ggggtggcgg cgccaggcgg aggagttgtc gatcacgacg ggcccctgcg
    19861 aggcgacctt ctcggccagc gccttggagg tggcgccgcc cgcggagaag agcacgatgt
    19921 ccaggcccga gtagtcggcc gtggccgcgt cctcgatcgt gatgtcctgg ccctgccagt
    19981 cgagggtctt gcccgcggac cgggcggagg cgaacagtcg cagctcgtcg accgggaact
    20041 tacgctcggc gaggatgccg cgcatgactg tgccgacctg accggtggct ccgacgattc
    20101 cgaccctcac gtgggctcct tactcgtact actcgtacct gtggacctgg gcagtcccgt
    20161 ttccatcatg cgtatgaccg gtatgcggtt gtccaatcca ttgtccggga tgcggaacgg
    20221 ggcgggtgcc gtgagatagc acacccgccc cgccctatat ccgtatcaat cgtgtcaatc
    20281 gcgacagatc accttagggg gtgaccttct cgatcgtgac gctgccgacc ccggcggctg
    20341 tgccccgggc gttcagcacc tggagctcgc cgacgaactg gcggccctcg ggcgcggcgc
    20401 ccgcgaccac gacatcggcg gccagctgcg cggactcgcc gttgcccagc ttcacggtcg
    20461 ccgacttgtc gaccttgagc tcaccgagcg agggcgcgta gtacacgtcc aggtagtcgt
    20521 agtcggtctc cccggccgga accgcgtaac cgtcgacgac gatggtgtac tcgcccgcgg
    20581 cgggatcggt cagggtgacc cgctcctcgg agtcgccgcc cgcggactgg ccgaccagct
    20641 tgtcgcccag gaacacggcc aggtccaggt cggcggacgc gtcggagacc ttgccgatct
    20701 tgatgtcgaa cagctcgaca ccctcaccga tggtcacctt gcgctcctgc gacgcgccgt
    20761 ccttgatgct cggacgctcc ttgacggacg agccgagcga gccgcccttc agcgtgccgt
    20821 cgatcgcggc gaaggtgttg gtgcccttcc actcgacctt ggccggagta ccgatcttgg
    20881 cctcgtcgag ggtcgcctcg gccgggtcga actccacgcc gagaaccgag acgtccagct
    20941 tgtacgggtt cttcagcagc ggcgacgtac ggcgcgactc gacctcgatc tcccagacgc
    21001 ccggctgcgg gttcgcgtac gagcgcaggt cggggcggca ggtgttggtc gggtgctcgt
    21061 agttcgggta gcagtggatc gtcgacgtgg agtccatcgg cacgccg
//
