LOCUS       NZ_WHOM01000047         2425 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 481, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000047
VERSION     NZ_WHOM01000047.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2425
                     /collection_date="2018-07"
                     /db_xref="taxon:2654677"
                     /geo_loc_name="China: Xinjiang"
                     /host="Apocynum hendersonii"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces apocyni"
                     /strain="TRM 66233"
                     /type_material="type strain of Streptomyces apocyni"
     gene            <1..>2425
                     /locus_tag="GCN81_RS12510"
     region          1..2425
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2425
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2425
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:>2425](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2425
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             1..>2425
                     /NRPS_PKS="Domain: ACP (120-193). E-value: 3e-34. Score:
                     109.1. Matches aSDomain:
                     nrpspksdomains_GCN81_RS12510_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (214-639). E-value:
                     1.1e-183. Score: 602.9. Matches aSDomain:
                     nrpspksdomains_GCN81_RS12510_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060789.1"
                     /locus_tag="GCN81_RS12510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_156724637.1"
                     /sec_met_domain="PP-binding (E-value: 2.1e-18, bitscore:
                     52.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 2.7e-234, bitscore:
                     763.7, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.9e-97, bitscore:
                     311.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.2e-17, bitscore: 50.4,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHAQGLPAQSLAWGLWNQTSTISGQLEEADLQRIQRLGLLPLSTA
                     EALDLFDAATTTGEPVLAVSRLDTAALRAQDTQPHPLLRGLAPKAPRRASSAQSGGSSL
                     AERLGGLSRDERERTLTDLVRTQVAAVLGHSDPSTIDAQRAFQELGFDSLTAVELRNQL
                     NNTTGLRLPTTLIFDHPNPTALATHLASELGGQEREFAPVAARADATSLEPIAIVGMAC
                     RYPGGVRSPEDLWRLVSEGADAVSEFPSNRGWDLDRLYDPDPDHTGTSYAREGGFLHDA
                     DQFDPEFFGMAPREALATDPQQRLLLETAWETLEHAGVLPAAVRGSRTGVFTGVMYHDY
                     GTQLAGAAPDDLEGYLASGNAGSVASGRVSYTLGLEGPAVTVDTACSSSLVALHLAANA
                     LRSGECDLALAGGVTVMASPTSFVEFSRQRGLAPDGRCKPFAAAADGTGWSEGVGLLLV
                     ERLSDAQRNGHNILAVVRGSAINQDGASNGLTAPNGPSQERVIRQALANAKLTTADVDA
                     VEAHGTGTRLGDPIEAQALLATYGQGRPEGRPLYLGSLKSNIGHSQAAAGVGSIIKVIE
                     SMRHGVLPRTLHVDEPTGHVDWEAGAVELLTEQLPWETDGRPRRAGVSSFGISGTNAHV
                     IIEAPASAAESEEPRETHESQESRSLPVLPWVLSGKSAEAVTAQAERLLSYVDEHPDLD
                     PLDVAFSLATDRTTFDYRAVVTGRDRDGLLDGVRALATGTPHPGVGTAARTTGGTAFLF
                     TGQGAQRVGMGQELYAAFPVFAEALDAACAALDPHLKRPLREVITSGDGLDRTEY"
     aSDomain        361..579
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS12510_ACP.1"
                     /evalue="3.00E-34"
                     /label="GCN81_RS12510_ACP.1"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="193"
                     /protein_start="120"
                     /score="109.1"
                     /tool="antismash"
                     /translation="TDLVRTQVAAVLGHSDPSTIDAQRAFQELGFDSLTAVELRNQLNN
                     TTGLRLPTTLIFDHPNPTALATHLASEL"
     aSDomain        643..1917
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS12510_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.10E-183"
                     /label="GCN81_RS12510_PKS_KS.1"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="639"
                     /protein_start="214"
                     /score="602.9"
                     /tool="antismash"
                     /translation="IAIVGMACRYPGGVRSPEDLWRLVSEGADAVSEFPSNRGWDLDRL
                     YDPDPDHTGTSYAREGGFLHDADQFDPEFFGMAPREALATDPQQRLLLETAWETLEHAG
                     VLPAAVRGSRTGVFTGVMYHDYGTQLAGAAPDDLEGYLASGNAGSVASGRVSYTLGLEG
                     PAVTVDTACSSSLVALHLAANALRSGECDLALAGGVTVMASPTSFVEFSRQRGLAPDGR
                     CKPFAAAADGTGWSEGVGLLLVERLSDAQRNGHNILAVVRGSAINQDGASNGLTAPNGP
                     SQERVIRQALANAKLTTADVDAVEAHGTGTRLGDPIEAQALLATYGQGRPEGRPLYLGS
                     LKSNIGHSQAAAGVGSIIKVIESMRHGVLPRTLHVDEPTGHVDWEAGAVELLTEQLPWE
                     TDGRPRRAGVSSFGISGTNAHVIIEA"
     CDS_motif       1129..1176
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS12510_0001"
                     /evalue="3.50E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="392"
                     /protein_start="376"
                     /score="22.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       1552..1596
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS12510_0002"
                     /evalue="1.50E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="532"
                     /protein_start="517"
                     /score="13.8"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       1657..1701
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS12510_0003"
                     /evalue="1.40E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="567"
                     /protein_start="552"
                     /score="11.2"
                     /tool="antismash"
                     /translation="GSLKSNIGHSQAAAG"
     CDS_motif       2257..2304
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS12510_0004"
                     /evalue="4.80E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="GCN81_RS12510"
                     /protein_end="768"
                     /protein_start="752"
                     /score="19.0"
                     /tool="antismash"
                     /translation="FTGQGAQRVGMGQELY"
ORIGIN
        1 cgccacgccc aaggcctgcc cgcccaatcc ctcgcctggg gactctggaa ccagaccagc
       61 accatcagcg gacaactcga agaggccgac ctccagcgca tccagcgcct cggtctgctg
      121 ccgctgtcga ccgccgaagc cctcgacctg ttcgacgccg ccacgaccac gggcgaaccc
      181 gtcctggccg tctcccgcct cgacaccgcc gccctacgcg cccaggacac ccagccgcac
      241 ccgctcctgc gtggcctggc cccgaaagcg ccgcgacggg ccagttccgc gcagagtggc
      301 ggctcctcgc tggccgagcg gctcggcggt ctctcccggg acgagcggga gcggacgctg
      361 accgatctcg tacggaccca ggtcgccgca gtgctcggtc acagcgaccc cagcaccatc
      421 gacgcacaac gcgccttcca agaactcgga ttcgactccc tcaccgccgt cgaactccgc
      481 aaccaactca acaacaccac cggcctccgc ctacccacca ccctcatctt cgaccacccc
      541 aaccccaccg cactcgccac gcatctcgca tccgaactcg ggggccagga aagggaattc
      601 gcgccggtcg cggcgcgtgc cgacgcgacg tcgctggagc cgatcgcgat cgtcggcatg
      661 gcctgccgct accccggcgg ggtgcgctcg cccgaggatc tgtggcgcct ggtgtccgaa
      721 ggggccgacg cggtcagcga gttcccgtcc aaccgaggct gggatctgga caggctgtac
      781 gacccggacc cggaccacac cgggaccagc tatgcccggg agggcgggtt cctgcacgac
      841 gcggaccagt tcgacccgga gttcttcggg atggcgccgc gtgaggcgct ggccaccgat
      901 ccgcagcagc ggctgctcct ggagaccgcg tgggagacgc tggagcacgc cggagtactg
      961 cccgccgccg tgcgcggcag ccgtaccggc gtcttcaccg gcgtgatgta ccacgactac
     1021 ggcacccagc tggcgggggc cgcgcctgac gacctggagg gctacctggc cagtgggaac
     1081 gcgggcagtg tcgcctcagg ccgggtctcg tacaccctgg gcctcgaggg gcccgcggtc
     1141 accgtggaca cggcgtgttc gtcgtcgctg gtggcgctgc acctggccgc gaacgccctg
     1201 cgctcgggcg agtgcgatct cgccctcgca ggtggtgtca cggtgatggc gtcgccgacg
     1261 tcgttcgtgg agttctcgcg gcagcggggg ctcgccccgg acggccgctg caagccgttc
     1321 gcggcggccg cggacgggac cggctggagc gagggcgtcg ggctgctgct cgtcgaacgg
     1381 ctctcggacg cccagcgcaa cgggcacaac atcctcgctg tcgtgcgcgg ttcggccatc
     1441 aaccaggacg gcgcgagcaa cggcctcacc gcgcccaacg gcccgtccca agagcgggtg
     1501 atccgccagg ctctggccaa cgcgaagctc accaccgccg acgtcgacgc cgtcgaggca
     1561 cacggcaccg gcacccggct gggcgacccc atcgaggcac aggccctcct cgccacgtac
     1621 ggccaggggc gcccggaagg tcgcccgctc tacctcggct cactcaagtc caacatcggg
     1681 cacagccagg ccgcggccgg agtcggcagc atcatcaagg tcatcgagtc gatgcggcac
     1741 ggcgtgctgc ccaggaccct gcatgtggac gagcccactg ggcacgtcga ctgggaggcg
     1801 ggcgcggtcg agctgctcac cgagcagctg ccgtgggaga cagacggccg cccgcgccgc
     1861 gcgggtgtct cctccttcgg catcagcggc accaacgccc acgtcatcat cgaggccccg
     1921 gcttccgccg ctgagtccga ggagccgcga gagactcacg aatcgcagga gtcgcgctcg
     1981 cttccggtgc tgccgtgggt gctctccgga aagtccgccg aagccgtcac cgcccaggcc
     2041 gagcgactcc tctcgtacgt cgacgagcac cccgacctcg acccgctcga cgtagcgttc
     2101 tccctggcca cggaccgaac gacgttcgac taccgggccg tggtgaccgg ccgcgaccgg
     2161 gacggactgc tggacggggt acgcgcactg gcgacgggaa ccccgcaccc cggcgtcggc
     2221 accgcggccc gcactacggg cggtacggcc ttcctgttca ccgggcaggg tgcgcagcgc
     2281 gtcgggatgg gccaggagct gtacgcggcc ttcccggtgt tcgctgaggc cctcgacgcg
     2341 gcatgcgccg cactcgaccc gcacctgaag cggccactgc gcgaggtgat cacctcaggc
     2401 gacgggctcg accgcaccga ataca
//
