LOCUS       NZ_WHOM01000046        21047 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 476, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000046
VERSION     NZ_WHOM01000046.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 176165)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 176165)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 83237
            Orig. end                         :: 104284
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21047
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21047
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21047
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11047](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11047
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    80..94
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.54"
                     /tool="antismash"
     misc_feature    complement(251..266)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            447..899
                     /locus_tag="GCN81_RS12085"
     CDS             447..899
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453772.1"
                     /locus_tag="GCN81_RS12085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4442 domain-containing protein"
                     /protein_id="WP_156724547.1"
                     /transl_table=11
                     /translation="MSADQMSIGEMLAATVPMARTLNLEFIETTPERAVLRLPDQPDYH
                     NHVGGPHAGAMFTLGESASGAIVIAAFGDQLSRAVPLAVSADIAYKKLAMGPVTATATL
                     GRPAADVVAELDAGQRPEFPVAIAIQREDGAVTGEMTVTWTLRPNS"
     gene            1054..3585
                     /locus_tag="GCN81_RS12090"
     CDS             1054..3585
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741988.1"
                     /locus_tag="GCN81_RS12090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="WP_156724548.1"
                     /transl_table=11
                     /translation="MSDTVSRRSALRLLSTAIAAATGASLASASPATAGPSRPSRPPRV
                     TALLDRLTLDEKISLLHGAEDPRSLGQAGYVPGVPRLGIPELRLADGPGGVRVREHATA
                     LPAPVLLASAFDPALAREYGQVIGREGRALGQDVLLSPMVNLIRTPYAGRNFETFSEDP
                     LLAADLVAEEIRGIQDAGLIATVKHFALNNQEKDRMTLDVVAGEQTMRETELRGFEAAV
                     AAGTGAVMGAYNKVNGTFACESRALLSDLLRDDWGFDGWVMTDWFAAHSTVAALDAGLD
                     MEMPSDVHFGVALRDAVRDGQVSEAAVDRAVGRVLAVLDRFGLLDGTAPARPARDAEAG
                     ARTALKVATAGAVLLRNERRALPLAGRAAQHIAVVGPTGSVPFVSGGGSAHVVPDHADS
                     PLDALTERAGSAARVTYALGEDLYGKPLPAAALTSGFAPEGQGVGPGETWQYDGTLTTG
                     EEDEWTFVVHYSGERPEVRLNGEELFPLAEGLAELFNGGLLPTAPDGLTVRRKTLALDG
                     GRHRLQITARGGDTGQLFRLRQVTGASRAQDVAEAVRVARAAHSVVLFAYEDATEGQDR
                     TTLALPGHQERLIEAVTAVNERTTVVLNTSSAVSMPWLERTAAVLQMYYPGQEGAAATT
                     AVLFGDADPGGRLTQSFPADDQAHPMAGDPRRYPGVDGVEEYSEGVRVGYRWYDAEGVP
                     PLFPFGHGLSYTTFAYEDLATHPATDGLAPTGGLAVSFTVRNTGVRDGVEVAQVYLGPS
                     PDLRVDQPERTLAGYRRLALKAGERRRITVRVARRTLSSWDTARHGWVLGTGPRTVWVG
                     ASSRDLRLSEQVRVQVQVPVRVDQSALSASS"
     gene            complement(3558..4103)
                     /locus_tag="GCN81_RS12095"
     CDS             complement(3558..4103)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988433.1"
                     /locus_tag="GCN81_RS12095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="WP_156724549.1"
                     /transl_table=11
                     /translation="MAERALIAGVGGKDPEVDPEAFTAPTSVVIGEVTLRPGASLWYHA
                     VLRADCGPIVLGADSNIQDNCTVHVDPGFPVTIGERVSVGHNATVHGCTVEDDCLIGMG
                     ATVLNGAVIGAGSLVAAQALVPQGMRVPPGSLVAGVPAKVRRPLTEEEREGIKVNAAMY
                     TELAKAHRAAHEDADSAD"
     misc_feature    complement(4099..4114)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.06"
                     /tool="antismash"
     gene            4265..5107
                     /locus_tag="GCN81_RS12100"
     CDS             4265..5107
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524815.1"
                     /locus_tag="GCN81_RS12100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyltransferase"
                     /protein_id="WP_156724550.1"
                     /transl_table=11
                     /translation="MPKYRNVFSSLAARRRRLVTCAVQAGWRWVRRAGAVTAERPGRLR
                     FRAIGTGTKLAFPQGTVFGEPWIELGDHCIVGEQVTLTAGMMPDLDLGPDPILRIGNGV
                     VLGRGSHVIADTTVTVGDDCYFGPQVYVTSTNHSYDDPHQPVGKQWPRMEPVEIGPGCW
                     VGTNAVILPGARLGRNVVVAAGAVVRGEVPDHAVVAGAPARVVRRWTPADGWQPPLRTP
                     APVPIPEGVTSEELLALAELPVEALAELPDELPDELTASGASGDVPDTAPGDRSAAGS"
     gene            complement(5126..5992)
                     /locus_tag="GCN81_RS12105"
     CDS             complement(5126..5992)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032087.1"
                     /locus_tag="GCN81_RS12105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_156724551.1"
                     /transl_table=11
                     /translation="MTALLALATSLLWGLADFGGGLLTRRTPALTVVVVSQCIAVAVLG
                     AIVLATGAWTEAGPKLWFAVAAGVVGPVAMLAFYKALALGPMGVVSPLGSLGVVVPLGV
                     GLVLGERPGLLQFAGIGVAVAGVMLAGGPQFRGAPVQRQAVLLTLVAAFGFGSVMALIS
                     EASTTLTGLFLALFVQRVANVVVGGGALYVSVRRGGRALPEDGGLAVIRASLPALAFVG
                     LADVAANGTYAIAAQNGPVTVAAVLASLYPVVTALAARGVLSERLRPIQATGAGLALIG
                     TLLLASG"
     gene            6088..6660
                     /locus_tag="GCN81_RS12110"
     CDS             6088..6660
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638350.1"
                     /locus_tag="GCN81_RS12110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_156724552.1"
                     /transl_table=11
                     /translation="MSDLDQLTQSLARSLKRWRGERGLTLDALAARSGVSRGMIIQIEQ
                     ARTNPSVGTTVKLADALGISITTLLDYEQGPEVKLVPEEQAVRMWWTEAGSWTKLLVGT
                     ETRGPFELWSWRLMPGDGSESDPHPAGTVELLRVTEGELTLVVDGEPYPVPAGTSASFE
                     SNVPHAYRNDGDAPVELYLAVSVPPAH"
     gene            6704..7261
                     /locus_tag="GCN81_RS12115"
     CDS             6704..7261
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991039.1"
                     /locus_tag="GCN81_RS12115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YbaK/EbsC family protein"
                     /protein_id="WP_156724553.1"
                     /transl_table=11
                     /translation="MRAPIGDFDDARPAPDCLDVLTRPVADAVRDWRGTVPAEQLLYVD
                     TDPAIADTAVFVEHHGAALLDQSANCVVVAGKRGGETSLAACVVLSRTRVDVNGVVRRQ
                     LGARKASFASMETATGETGMEYGGITPIGLSASWPLLVDAAVVDTEYVLIGSGGRRGKL
                     IVPGKIFAELPGAVVLDGLGVS"
     gene            complement(7308..7715)
                     /locus_tag="GCN81_RS12120"
     CDS             complement(7308..7715)
                     /GO_function="GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131683.1"
                     /locus_tag="GCN81_RS12120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CoA-binding protein"
                     /protein_id="WP_156724554.1"
                     /transl_table=11
                     /translation="MYGDKETIDRVLTELGDTWAVVGLSNNRARVAYDVAEVLQRHGKR
                     IVPVHPKAETVHGERGYATLADVPFEIDVVDVFVNSALAGAVADQAAAAGAKAVWFQLG
                     VIDEAAYARTRAAGLDMVMDRCPAIEFGRRY"
     gene            complement(7805..9634)
                     /locus_tag="GCN81_RS12125"
     CDS             complement(7805..9634)
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301849.1"
                     /locus_tag="GCN81_RS12125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_156724555.1"
                     /sec_met_domain="Pkinase (E-value: 1.4e-41, bitscore:
                     135.9, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEQRDADGGTALHQAGADRLQPGDPHQIGPYAPLGHLGSGGMGR
                     VYLARPADNRSGLAAVKVIRPEFAQDAEFRRRFEREASVLARVRGTHAPRLLDTGFDGD
                     LLWMATDYLPGLNLSDAVREHGLLAAPGAWRLLGELGQALAELSAAEVVHRDLKPSNVI
                     LSGDGAQVIDFGISQATDSTAITTTGKAVGTPAYMSPERLREGRSDATSDVFSLACTLV
                     FAATGRAPFGDGTGVDVMHRVAYEDPDREVIGELAAVDPALASLLSACLAKEPSQRPTP
                     HHLIDAAAARPRTPQWQEPLGSHLAARRQAYEVLKDLPVERTVHLRLPSPAGPSPAPPP
                     PAPPGFGPPLQAPAQAPMPAPAQAQAQAQAPAQTPAPGKRRKGLYLSGAAGVAVCAVAL
                     TAFVLTRPDTGTGTDRTEAAGTRSSAPADDSRAADDAAPVPGFSPDGSDSSEKAPGDEK
                     ESGKGKPDQDNEAEKESDGEQGGESAAPQPDRVSSAPSSTASRTSPAQPGATSSPTAPA
                     KSTPTAPSTPPWLSDCTHYSGTQITHYGHRGQRVLQVQCMLSKRGYSVGGSGVDGEFGE
                     DTRSAVRLFQSDRKLAVDGEVGPNTWSALRDRT"
     misc_feature    complement(9860..9878)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.24"
                     /tool="antismash"
     misc_feature    9863..9877
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.03"
                     /tool="antismash"
     misc_feature    9870..9884
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.16"
                     /tool="antismash"
     misc_feature    9945..9956
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.53"
                     /tool="antismash"
     gene            10001..11047
                     /locus_tag="GCN81_RS12130"
     CDS             10001..11047
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742346.1"
                     /locus_tag="GCN81_RS12130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="terpene synthase family protein"
                     /protein_id="WP_156724556.1"
                     /sec_met_domain="T1TS (E-value: 6.2e-64, bitscore: 210.3,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALLSSFAIPDFHLPFESTKHPQAEQANTEATSWALSQGLIKDAA
                     DDFSGIGCGHLAARVAHGATYRQLLLLSEWMAWSFVLDDQHDHVIRSGRVDTWRPVTDA
                     INGHLSDGEDYLTPQRRHGNALVDGFTDLHAAILDGMPPGIRKRYRAHIPPMLRSLDQE
                     AVNRGTVRRQPTVDDYVLMRRHSSQLLPMMDMVEASLGVEVPSEVYDSPTFQALLWSAV
                     DIISWGNDIFSLRKEYACGDNNNLVLLLAWWEGYSLPHAVRAVKDRISARLVDFHSAAR
                     TLPRVLDVLAITDPATREGVARCVRSYEDWIIGADLWQRHECTRYSDERWVSGDEGAYT
                     RPDLVAVA"
     gene            11044..12414
                     /locus_tag="GCN81_RS12135"
     CDS             11044..12414
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742345.1"
                     /locus_tag="GCN81_RS12135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_156724557.1"
                     /sec_met_domain="p450 (E-value: 7.6e-82, bitscore: 268.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGGQAIAPTPHEPRALDAPVAPGKLPVLGHAIPLWRRPIAFLES
                     LRTVGGVVRIDLATWPVYVLTSPDLVDAVLVSEARHFGRGRVFDKLRPLFGNGIVTTDG
                     EFHRKQRRLMQPAFHRAQLTAYAEVMAQQADAMAASWTAGQELSIVTEMRRFALGSVAK
                     MIFSGDMGRPAVAEVHRSLPVVLEGMLVRAVMPKALDRLPIPLNRRFDAAAARLRRIID
                     EVIVEYRAEQEDRQDLLSLLLSAVDADTGERMSDEQVRDEVIAIMFAGTETSATVLSWI
                     FHELGRDPEARKRLQAEVDGVVGSGPVRPDHVPQLTYTHHVFHEALRLHSPLLFTRRAL
                     APVNLAGVAIPAGAEVAYSPYALHRDPALFPQPQRFQPERWEKSPKRRPHLNSFIPFGA
                     GQHKCIGDHFAAAEIVIAVASVMSRWDVVHAPGVTVREVAAGIPQPDELTMMPVARQ"
     gene            12540..15440
                     /locus_tag="GCN81_RS12140"
     CDS             12540..15440
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058744.1"
                     /locus_tag="GCN81_RS12140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_156724558.1"
                     /transl_table=11
                     /translation="MTQEQSAEQPRWTTPPVLERESELHAIRRALDDLCGTSPDGAGTR
                     HGGVLAFAGPAGAGKTTLIAELRRQATERGCAVLFARGGEQERQVPFHVVRQLVQPALA
                     SMDDSERREILGHWYGIVAPAIGLTVSQGVAAPDPQGVRDGLDWLVTHLSVRNAPVLLV
                     LDDAHWADAESLTWLTAFAARAEELALLIVVAHRPEELLADVAGLQALAERHGARPHQL
                     TALTTTAVAHLVRGALGDDADDTFCREFWAITGGNPFEVVELMAKARERALRPTEGSIP
                     QLRDLASAVKGNGLVERLETLGPSTVRLAWAAALLGSGHSTELVGAVAALGEAQTADAI
                     ERLHDARILTSLHHIRRGKVVEFFHPLVATAVYRAIPAAVRVAMHGIAANALIDAGHGP
                     TAAARHILEMHTEGDPWVVQQLRQAARDNFASGAPDAALRCLTRALREPPDVEDRAQVL
                     FELGSAGLFYDPATAVNHLRAALEEPKLDQGLREAVSYRLAQALAHVGQMGQATDLLAE
                     EARRTTSARTRLRMQAEQFKWNAVRVDEEDSPARSRLLAQFAKRLTGRDLAERHILGLR
                     AWDSVMRGEPAAQALQFAEQALDGGLSWTDQDFGFEVPVVVALTFMYGDQPGRAEELFH
                     KGIAEFEERGWRGAHLSFAYTLLGYIRYRRGRLAEAEDYVRNGLQIANRVGQNIPAQWY
                     AIGTLIETLIARGDIDEAQNVAEAYDFGKDFPKAVVYPDPQAVYGKLLLAHGARDQAER
                     HLAAAGRRLDRRGSRNPAWSPWQLDLALAQAEYDPDQARATADAAVTRARVFGTASAKG
                     QALRVAAATDPARAAMLLHEAVEHLEQSPAAYELAHALIDHGAALYAEGNLEHASRQYH
                     RGLETATACGADALATRARTELGAAGLRPQHPQTAAQDTLTEAESAAARHAVRGLDNAA
                     IAAELRLDEHAISRLLSAVFAKLGTDRTGLSRALGG"
     gene            15668..17173
                     /locus_tag="GCN81_RS12145"
     CDS             15668..17173
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262596.1"
                     /locus_tag="GCN81_RS12145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FUSC family protein"
                     /protein_id="WP_156724628.1"
                     /transl_table=11
                     /translation="MFVAPDPGLLRLRVSLRAVLGVGLAVAAAEVAGLSLSASITGGLA
                     ALLALFTVGDATVRGQAVTTALLPVVGLPVLALAAALHGMPMARDAAFVAVVFCGVYAR
                     RWGPRGHALGIFAFMQFFVAQFLHAVPAQLPELYAAVALALAAAAAVRFGAWCVERRTP
                     LPMPPGPLAGAGLDRPSTRQAFQATAACAFAVVIGQLVSGDRWYWAVGTAWWIFVNTAS
                     RGETLVRGFRRVLGTVVGIAAGLLIAVPLHGAPGPTAALVAVCVFGIFYTAAPSYSWMM
                     FFVTIMAGLLYGLLGVLHPGLLLLRFVETGVGALGAALAVLLVLPITTHAATEAWIQRA
                     LREVHRCTSEAARRLAGDENADPAPHAAALEALLGRVRMALAPLVHPLSPLRARKARAR
                     QILVLLDACAREVRGLAVVAADPDASHDVRLTEACHRVAVALEALVDTEEALAESFGPR
                     PATVPRHDPGAEQALAHLRELEQALTGLATPLRREPRPVLATT"
     misc_feature    17177..17192
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.99"
                     /tool="antismash"
     gene            17235..18353
                     /locus_tag="GCN81_RS12150"
     CDS             17235..18353
                     /EC_number="3.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118342.1"
                     /locus_tag="GCN81_RS12150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactonase family protein"
                     /protein_id="WP_156724559.1"
                     /transl_table=11
                     /translation="MGGMRAYIGSFTSAGGRGITTASIDEDTGALTALSATDVLADPSY
                     LALSDDGEVLYAVSESERGQVAAFGVRAERPEPLGAPVAVGGASPTHLALAAGHLVTAN
                     YGSGSVSWLPVRAGGQLGAAGAGAGVLRHTGSGPDPERQRGPHAHQVLPDPSGRWVLSV
                     DLGTDSVRVCSLVASEAVAGKAVEGGAVTGEAITGEAGALAELRTHREVVLRPGSGPRH
                     LAFHPRGDLAYVVHELSATVTCCRWDAQAGHLEPLGETRLLHGGPVAGDAYPSGLVVST
                     DGRFLWAATRGQDVISVLALDGDGVRPRLVTTVASGGHWPRDLALHPSGRFLYAAHERS
                     GDVTWFAVDPESGALRRAGAIEAPAASCVVFG"
     assembly_gap    18381..18401
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18459..19379)
                     /locus_tag="GCN81_RS12155"
     CDS             complement(18459..19379)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982967.1"
                     /locus_tag="GCN81_RS12155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156724560.1"
                     /transl_table=11
                     /translation="MSSPTGPASGLPVRMPRPRQPGRHRRPEPAVAPEGAPALVLAVPG
                     VPSTATRSLAEEVVSIARSELPGMNARIGYLDGDDEEFPSLRSVLAQTAEQRTARYELA
                     LAAGREVAEPEGPAAVVVPLLAGPDAALMGQIRQAVVDSGAPAELTDVLGPHPLLAEAL
                     HVRLSEAGLARADRARLFTVATAADGIILATVGGEEAVQAAGISGMLLAARLAVPVMAA
                     ALDQEGAVAATAEQLRGSGSTQLALAPHVIGPEVDEGLLEVAAKDAGCASAEALGAYPA
                     IGRLALSQYMTALGIAPQPTGAPAQ"
     gene            complement(19475..20551)
                     /locus_tag="GCN81_RS12160"
     CDS             complement(19475..20551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970817.1"
                     /locus_tag="GCN81_RS12160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylglucosamine kinase"
                     /protein_id="WP_156724561.1"
                     /transl_table=11
                     /translation="MRSRRHTPEGDGGPSCVLGVDSGGSGLRVALGVTGGGLRQVEPVM
                     SREPVRTGSGGIDADHLLEQLTPMAAGLIESAGGGGGTVVEAVVIGAAGMATLGGELRA
                     KLPSALTATLGVRRLALAADAVTAYAGALGQRPGAVVAAGTGMVALGTDLTRWRRADGW
                     GHLLGDCGSGAWIGRAGLEAALRAYDGRRGGSRALLARARETFGPVSELPGQLYPRGDR
                     PAVLASFAPSVAECAAHDRVAAGILAAAARHIAEAAAAVCPAPAPVLAPDPAPDPAPDG
                     SDEPPEVAFTGGLFRLGEPLLVPLRAELAEQLPHARLVPAAGDPLDGAVRIALDLAGGG
                     TALPPDPHLLYIPKETDS"
ORIGIN
        1 tcgtcgtcat cgccgccgag caccaggtac cccgtcacgc ccgccgctgc cacgaccgcg
       61 gtcgccgcga cgatggcgat gatcgtcgtc ttcttgcggt cgtcacccgg cggcttggcg
      121 acggcgccca tcggggtcgt cggcgcgttc cacggaccct gcggctgggc gccgggctgc
      181 tgctgcgtgc cgggctgctg ctggtagccc ggctgcgtgt acgggttctg ttgctggtat
      241 cccggctgct ggtagggatt ctgattcgga tcctgcgggt tctgctcgcc cccgggcggc
      301 tgctgtcctg gccacatggc gagaacgata gagggccggg tagcggggtt ccatcgccgc
      361 ccccgccttg tgacgggctt ggtacacctg ttccaaggat gtgaggggat ggtcaagacg
      421 tcctactggt gggtaacatc gccgccatga gcgctgacca gatgtcgata ggcgagatgc
      481 tcgccgccac cgtgccgatg gcccggaccc tgaacctcga gttcatcgag accaccccgg
      541 agcgtgccgt gctccgactg ccggaccagc cggactacca caaccacgtc ggtggcccgc
      601 acgctggcgc catgttcacg ctgggcgagt ccgcgagtgg cgcgatcgtg atcgccgcgt
      661 tcggtgacca gctctcgcgg gccgtaccgc tcgcggtcag cgccgacatc gcgtacaaga
      721 agctggccat ggggccggtg accgccaccg cgacgctcgg ccgtccggcg gccgacgtgg
      781 tcgcggagct ggacgcgggt cagcgcccgg agttcccagt ggccatcgcc atccagcgcg
      841 aggacggcgc agtgaccggc gagatgaccg tgacctggac tctgcgtccc aacagctgag
      901 acaggctcag cagcggcacg gatacacagc ggcggacgcg ccgaccgaac ccctccgggg
      961 cggtcggcgc gtccgccgtt tcgtgtggtg ggtcttccgt cacgatcgga gtgtccaccg
     1021 aaggacagag acggtcagca ggaaggacgg cttatgtccg acaccgtgtc caggcggtcg
     1081 gcgctacggc tgctgagcac cgcgatcgcg gcagccacgg gcgcgagtct cgccagcgcc
     1141 tcgcccgcga cggccggacc ctcccgcccc tcccgccccc cgcgcgtcac cgcgctgctg
     1201 gaccggctca ccctcgacga gaagatctcg ctgctgcacg gcgccgaaga cccgcggagc
     1261 ctcggccagg ccggatacgt gcccggcgtg ccccgcctcg gcatccccga gctgcggctc
     1321 gcggacggcc ccgggggcgt acgggtcagg gagcacgcca ccgcgctgcc cgcgcccgtg
     1381 ctgctcgcct ccgccttcga ccccgcgctg gcccgcgagt acggacaggt catcggccgg
     1441 gaggggcggg cgctgggcca ggacgtcctg ctgtcaccga tggtcaacct catccgcacc
     1501 ccctacgcag ggcggaactt cgagacgttc agcgaggacc cgctgctcgc cgctgacctg
     1561 gtggccgagg agatccgggg catccaggac gccgggctca tcgccaccgt gaagcacttc
     1621 gcgctgaaca accaggagaa ggaccggatg accctcgacg tggtcgccgg ggagcagacg
     1681 atgcgcgaga ccgaactgcg gggcttcgag gccgccgtcg ccgccgggac cggtgccgtg
     1741 atgggcgcgt acaacaaggt caacggcacc ttcgcctgcg aaagccgggc cctgctcagc
     1801 gatctgctgc gcgacgactg gggttttgac ggctgggtga tgaccgactg gttcgcggca
     1861 cacagcaccg tcgccgcgct cgacgccgga ctcgacatgg agatgccgag cgacgtccac
     1921 ttcggcgtcg cgttgcggga cgccgtgcgg gacggccagg tgtccgaggc cgccgtggac
     1981 cgtgccgtgg gccgggtcct ggcggtgctc gaccgcttcg ggctgctgga cggcacggcc
     2041 ccggcacggc ccgcccggga cgccgaggcc ggagcacgga ccgcgctcaa ggtggccacg
     2101 gcgggcgcgg tgctgctgcg caacgagcgc cgcgccctgc cgctggccgg gcgcgcggcc
     2161 cagcacatcg ccgtcgtcgg gcccaccggg tccgtcccct tcgtcagcgg gggcggcagc
     2221 gcgcatgtgg tccccgacca cgccgacagc ccgctcgacg ccctcaccga acgcgcgggc
     2281 agcgccgcgc gggtgacgta cgcgctgggc gaggacctct acggcaagcc gctgcccgct
     2341 gccgcactga cctccgggtt cgccccggag gggcaggggg tcgggccggg ggagacctgg
     2401 cagtacgacg ggaccctcac caccggggag gaggacgagt ggacgttcgt cgtccactat
     2461 tcgggtgagc gccccgaggt ccggctgaac ggcgaggaac tcttcccgct cgccgaaggg
     2521 ctcgctgaac tcttcaacgg cggactgctg ccgaccgcgc ccgacgggct caccgtgcgc
     2581 cgcaagaccc tcgccctcga cggcggacgg caccggctcc agatcaccgc gcggggcggc
     2641 gacaccggcc agctcttccg gctgcggcag gtcaccggag cgagccgggc ccaggatgtc
     2701 gccgaggcgg tacgggtcgc gcgcgccgcg cacagcgtcg tgctcttcgc ctacgaggac
     2761 gccaccgaag gccaggaccg tacgacgctc gcgctccccg gacaccagga acggctcatc
     2821 gaggccgtga cggccgtcaa cgagcggacc acggtcgtcc tcaacacctc gtcggcggtc
     2881 tcgatgccgt ggctggagcg gaccgccgcc gtcctccaga tgtactaccc gggacaggag
     2941 ggcgcggcgg ccaccaccgc ggtcctgttc ggcgacgcgg accccggcgg gcgcctcacc
     3001 cagtccttcc cggccgacga ccaggcgcat ccgatggccg gggacccgcg ccggtatccg
     3061 ggtgtggacg gggtcgagga gtactccgag ggcgtacggg tcggctaccg ctggtacgac
     3121 gccgaagggg tgccgccgct gttcccgttc gggcacggcc tgtcgtacac gaccttcgcg
     3181 tacgaggatc tggcgacgca cccggcgacc gacggtctcg ccccgaccgg cggcctcgcc
     3241 gtcagcttca ccgtgcgcaa caccggtgtc cgcgacggcg tcgaggtcgc ccaggtctac
     3301 ctgggcccgt cccccgacct gcgggtcgac cagcccgagc ggaccctcgc cggataccgc
     3361 aggctcgccc tgaaggcggg cgagcggcgc cggatcaccg tacgggtcgc gcgccggacc
     3421 ctgtcctcgt gggacaccgc acgtcacggc tgggtgctgg ggacggggcc gcgcacggtg
     3481 tgggtgggtg cctcgtcccg cgatctgcga ctcagcgagc aggtccgcgt ccaggtccag
     3541 gtcccggtcc gcgtcgatca gtccgcgctg tccgcgtctt cgtgagccgc gcggtgcgcc
     3601 ttggccagct cggtgtacat cgccgcgttg accttgatgc cctcgcgctc ttcctcggtc
     3661 agcggccgcc tgaccttggc gggcacaccg gcgaccagtg agccgggcgg cacccgcatc
     3721 ccctgcggca ccagcgcctg ggccgcgacc agcgaaccgg cgccgatcac cgcgccgttg
     3781 agcaccgtgg cgcccatgcc gatcaggcag tcgtcctcga cggtgcagcc gtgcaccgtg
     3841 gcgttgtggc cgaccgacac gcgctcgccg atggtgaccg ggaacccggg gtcgacatgg
     3901 acggtgcagt tgtcctggat gttgctgtcg gcgccgagca cgatcgggcc gcagtcggcg
     3961 cgcagcaccg cgtggtacca gaggctcgcg ccggggcgca gcgtcacctc gccgatcacg
     4021 acggacgtgg gggcggtgaa ggcctccggg tccacctcgg ggtccttgcc gccgactccc
     4081 gcgatcaacg cccgctctgc cattgcttct ccttgttgcc agtgccgtgc catacggtgc
     4141 ccgtacgcct gtcacgccca ctggcaccgt aagcgacccg gcgtcccgcc cgccgacccg
     4201 ggtggggtga agatcacagc ccgctccgcc cgcgcctcgt atcggcgctg agtaccgtgt
     4261 gcgagtgccg aagtacagga acgtgttctc atcgctggcc gcacggcggc ggcgcctggt
     4321 gacgtgcgcg gtccaggccg gttggcgctg ggtgcggcga gcgggcgcgg tgaccgcgga
     4381 gcggcccggc aggctgcgct tccgcgcgat cggcaccgga acgaagctgg cgtttccgca
     4441 gggcacggtg ttcggcgagc cgtggatcga gctgggcgac cactgcatcg tgggcgagca
     4501 ggtcacgctc accgccggga tgatgcccga cctggacctc gggcccgacc ccatactcag
     4561 gatcggcaac ggcgtggtgc tcggccgtgg cagccatgtc atcgccgata cgacggtgac
     4621 cgtcggggac gactgctact tcggcccgca ggtgtacgtc acgtccacga accacagtta
     4681 cgacgatccg caccagcccg tgggcaagca gtggccgcgg atggagccgg tggagatcgg
     4741 ccccggctgc tgggtcggca ccaacgcggt gatcctgccg ggcgcgcgac tcggccggaa
     4801 cgtcgtggtg gcggcgggcg cggtggtacg tggcgaggtg cccgaccacg ctgtggtggc
     4861 cggggcgccc gcgcgggtgg tccgccgctg gaccccggcg gacggctggc agccgccgct
     4921 gcgtactccg gcgccggtgc cgatcccgga gggcgtcacg tccgaggaac tgctggcgct
     4981 ggccgaactg ccggtcgaag cgcttgccga gctgccggac gagctgccgg acgagctgac
     5041 ggcgagcgga gcgtcgggcg acgtaccgga tacggcgccg ggggaccggt cggcagccgg
     5101 gtcgtagccg cccggcctgg ccccgtcagc cgctcgccag cagcaaggtg ccgatcagcg
     5161 ccagccccgc gccggtcgcc tggatggggc gcagccgctc gctgaggacg ccacgtgcgg
     5221 cgagcgcggt caccacggga tagagcgagg ccagtacggc ggccacggtg accggaccgt
     5281 tctgcgcggc gatcgcatag gtgccgttgg cggccacatc ggcgagcccg acgaacgcga
     5341 gcgccgggag cgaggcgcgg ataaccgcga gaccgccgtc ctcgggcagc gcccgcccgc
     5401 cgcgccgcac cgacacgtac agcgcgccac cgcccaccac cacgttggcg acccgctgta
     5461 cgaacagggc gaggaacaga cccgtgagcg tcgtcgacgc ctcggagatc agcgccatca
     5521 cggagccgaa cccgaacgcg gcgaccaggg tcagcagaac cgcctgccgc tgcaccggag
     5581 ccccccggaa ctgcggccca cccgcgagca tcacccccgc gaccgccacg ccgatccccg
     5641 cgaactgcag cagcccgggc cgctcaccca gcaccaggcc gacgccgaga gggacgacca
     5701 cccccagcga gccgagcggc gagacgacgc ccatcggccc gagcgccagc gccttgtaga
     5761 acgcgagcat cgccaccggg cccaccacac cggccgccac cgcgaaccac agcttcggcc
     5821 ccgcctcggt ccaggccccg gtcgcgagca cgatcgcccc gagcaccgcc acggcgatgc
     5881 actgggagac gaccaccacg gtgagcgcgg gcgtacgtcg cgtgagcagc ccgccgccga
     5941 agtcggccag cccccacagg aggctggtgg ccagggcgag cagagcggtc atgaggggaa
     6001 cctcgcagta cagtgtggtg aaccttcggg tgtaccacac cgtagtgcat tgtagtgaac
     6061 tatcgcatcc aaaatattgg acggaatgtg tcggatctcg accagctcac ccagtcactc
     6121 gcccgcagcc tcaagcgctg gcgcggcgaa cggggcctca ccctcgacgc gctggccgcc
     6181 cgctcgggtg tcagccgcgg catgatcatc cagatcgagc aggcccgtac gaacccgagt
     6241 gtgggcacca cggtgaagtt ggcggacgcg ctggggatca gcatcaccac gctgctcgac
     6301 tacgagcagg ggcccgaggt caagctcgtg cccgaggagc aggccgtacg catgtggtgg
     6361 acggaggccg ggagttggac caaactcctg gtcggcaccg agacccgggg gccgttcgag
     6421 ctctggtcct ggcggctgat gcccggcgac gggagcgagt ccgacccgca tcccgcgggg
     6481 acggtcgagc tgctgcgggt caccgaaggc gagctcaccc tcgtcgtgga cggtgagccg
     6541 taccccgtac cggccggaac gtccgccagc ttcgagtcca acgtcccgca cgcctaccgc
     6601 aacgacggcg acgcgcccgt cgagttgtac ctggccgtgt ccgtcccgcc cgcgcactga
     6661 gacattccgg cgaagtcccg ggtagggctg ttaacttgcc gccatgcgcg cacccatcgg
     6721 agacttcgac gacgcccgcc cggcccccga ctgcctggac gtgctgacgc ggccggtggc
     6781 cgacgccgta cgcgactggc gcggcacggt gcccgccgag cagctgctct acgtcgacac
     6841 cgaccccgcg atcgccgaca ccgccgtctt cgtcgagcac cacggagccg cgctcctgga
     6901 ccagtcggcg aactgcgtgg tcgtcgcggg caagcgcggc ggtgagacca gcctggcggc
     6961 ctgtgtggtc ctctcccgca cccgggtcga cgtcaacggc gtcgtgcgcc gccaactcgg
     7021 agcccgcaag gcctcgttcg cctcgatgga aacggccacc ggcgagaccg gcatggagta
     7081 cggaggcatc acaccgatcg gtctgtccgc gtcctggccg ctgctggtgg acgccgccgt
     7141 cgtggacacg gagtacgtcc tgatcggcag cggcggccga cgcggcaagc tcatcgtgcc
     7201 gggcaagatc ttcgcggagc tgcccggcgc ggtcgtcctg gacgggctcg gcgtcagctg
     7261 agccggtccg aggcgagggc cggtgttctc agtaccgccg actgttctca gtaccgtcgg
     7321 ccaaactcga tcgccgggca gcggtccatc accatgtcga gcccggccgc gcgggtgcgc
     7381 gcgtacgccg cctcgtcgat gacgccgagc tggaaccaga cggccttggc gcccgcggcg
     7441 gcggcctggt ccgccacggc gcccgccaga gcgctgttca cgaacacatc gacgacgtcg
     7501 atctcgaacg gcacgtccgc gagggtggcg tatccgcgct ctccgtgcac ggtctccgcc
     7561 ttcgggtgca ccgggacgat gcgctttccg tgccgctgga gtacttcggc gacgtcgtag
     7621 gccacgcggg cgcggttgtt ggacaggccc acgacggccc aggtgtcgcc gagctcggtc
     7681 agcaccctgt cgatcgtctc tttgtctccg tacacagctg cctcccgtgg ctggtgtcct
     7741 ttctgggcaa cagcgcgcgc cgagcgtcga ttcccggtca cggcccgggc gtcggggcct
     7801 ggggtcacgt cctgtcgcgc agggcgctcc aggtgttggg ccccacctcg ccgtcgacgg
     7861 ccagtttccg gtcgctctgg aagagccgca ccgccgagcg ggtgtcctcg ccgaactctc
     7921 cgtccacacc ggagccgccg acgctgtagc cgcgcttgct cagcatgcac tgcacctgga
     7981 gtacgcgctg gccccggtgg ccgtagtggg tgatctgggt tcccgagtag tgcgtgcagt
     8041 cggagagcca ggggggcgtg gagggagcgg tgggagtcga cttcgcgggc gcggtggggg
     8101 agctggtggc gccgggctgt gccggagacg tacgggaagc ggtggagctg ggggcgctcg
     8161 atacacggtc gggctggggt gcggcgctct cgccgccttg ctcgccgtcg gattccttct
     8221 ccgcctcgtt gtcctggtcc ggcttcccct ttccgctctc cttctcgtcc cctggggcct
     8281 tctccgagga atcggagccg tccggcgaga agccgggcac gggtgcggcg tcatcggcgg
     8341 cgcgggagtc gtcggcgggt gcgctggacc gcgtgcccgc cgcctcagtg cggtcggtgc
     8401 cggtgccggt gtcggggcgg gtcaggacga aggcggtgag ggcgacggcg cagaccgcga
     8461 ccccggccgc gccggacagg tagaggccct tgcggcgctt gcccggcgcc ggcgtctgtg
     8521 ctggtgcctg tgcctgtgcc tgtgcctgtg ctggtgctgg catcggcgct tgcgctggtg
     8581 cctgaagggg cggcccgaag cccgggggag caggcggcgg aggagcgggt gacggtcccg
     8641 cgggcgacgg gaggcggaga tggaccgtcc gctcgacggg gaggtccttc agcacctcgt
     8701 aggcctgacg acgcgcggcc aggtgggagc cgagcggctc ctgccactgg ggcgtacgcg
     8761 gccgtgccgc cgccgcgtcg atcaggtggt gcggggtggg gcgctggctc ggctccttgg
     8821 ccaggcaggc ggacaggagt gaggccaacg cggggtcgac cgccgcgagt tcgcctatca
     8881 cctcgcggtc cgggtcctcg tacgccaccc ggtgcatcac gtccacgccc gtgccgtccc
     8941 cgaagggggc ccgtccggtc gcggcgaaga ccagggtgca ggcgagcgag aacacgtccg
     9001 acgtggcatc ggaccgccct tcccgcaggc gttccggtga catgtaggca ggggtgccga
     9061 cggccttgcc ggtggtggtg atggcggtac tgtccgtggc ctgcgagatg ccgaagtcga
     9121 tcacctgcgc gccgtccccg gacaggatca cgttggacgg cttgaggtcg cggtgcacca
     9181 cctcggctgc ggacagttcg gccagtgcct gcccgagctc gccgagcagc cgccaggctc
     9241 ccggcgccgc cagcaggccg tgttcgcgta cggcgtccga gaggttgaga cccggcaggt
     9301 agtccgtggc catccagagc aggtcaccgt cgaagccggt gtccaggagc cgtggcgcgt
     9361 gcgtaccgcg cacccgcgcc agtacggacg cctcccgctc gaaccgccgc cggaactcgg
     9421 cgtcctgggc gaactcgggc cggatcacct tgaccgccgc gagcccggag cggttgtccg
     9481 cggggcgcgc gaggtagacc cggcccattc cgccactgcc caagtgcccc agcggcgcgt
     9541 acgggccgat ctgatgcgga tcgccgggct gcaggcggtc cgctccggcc tgatggagcg
     9601 cggtgccccc gtcggcgtct cgctgctccg ccatggtgtg tgcccccgta ctcgtgctga
     9661 tgcgcccccg gaagggtgag gttatcgcag cctgagcacg ggtctcccag gagccacagg
     9721 cgccccacag gccgcgtgtc ctgggcggtg tggggctcgt gcgccccaac tgcccctggg
     9781 cgagcgagag tatggcacat gcatatgcct aatgaagtga cgggttggca attgccctat
     9841 ctgtcctggt atttggcgtc gcaatctgtt gacaatcttc ttcagggtga gcgttaatgg
     9901 tcatgtggct tgtttgatgt gtcgttcgtg cgcgtgaatt gatcgaaatt gttttgcgtg
     9961 agcggggagt ggctcatcca gatgcttgct ggaggtagaa atggccctgc tcagctcttt
    10021 cgcgatcccg gacttccatc ttccgtttga gagcaccaaa cacccccaag cagaacaggc
    10081 caatacggag gccacctcct gggcgctcag ccagggtctg atcaaggacg cggcagacga
    10141 tttctccggc atcggctgcg gccacctcgc ggcgcgggtc gcccatgggg cgacgtaccg
    10201 tcaactcctc ctgctctccg agtggatggc ctggtcgttc gtgctcgacg accagcacga
    10261 tcacgtcatc aggtccggtc gggtcgacac ctggcggccc gtaaccgacg ccatcaacgg
    10321 ccacctgagc gacggcgagg actacctgac cccgcagcgg cggcacggga acgcgctggt
    10381 ggacggcttc accgacctgc acgccgccat cctggacgga atgcccccgg ggatacggaa
    10441 gcgctaccgg gcgcatatac cgcccatgct gcgctccctg gaccaggagg cggtcaatcg
    10501 cggaaccgta cgacgtcagc caacggtcga cgactacgta ctgatgcgcc gccacagctc
    10561 ccaactgctg cctatgatgg acatggtgga ggcctcactc ggcgtcgagg tgccgagcga
    10621 ggtctacgac agcccgacgt tccaagctct cctgtggagt gccgtggaca tcatctcctg
    10681 gggcaacgac atcttctccc tgcgcaagga atacgcctgc ggggacaaca acaacctcgt
    10741 tctgctgctg gcctggtggg agggctactc gctgccccac gccgtgcgcg ccgtcaagga
    10801 ccgcatcagc gcccgcctgg tggacttcca ctccgcggcc cgcacactgc cacgggtcct
    10861 ggacgtgctc gccatcaccg acccggccac gcgcgaagga gtggcgcggt gcgtgcggag
    10921 ctacgaggac tggatcatcg gcgccgacct ctggcagcgg cacgagtgca cccgctacag
    10981 cgacgaacgc tgggtgagcg gagacgaagg cgcgtacacc cggccggact tggtggccgt
    11041 ggcatgaccg gcggccaggc catcgcgccc accccccatg aaccccgcgc ccttgatgcc
    11101 ccggtggccc cggggaagct gcccgtgctc ggccacgcga tcccgctgtg gcgccgcccg
    11161 atcgcgttcc tggagtcgtt gcgtacggtc ggcggggtgg tacggatcga tctcgcgacc
    11221 tggccggtgt atgtcctcac cagcccggac ctcgtcgacg ccgtactggt cagcgaggcg
    11281 cggcacttcg ggcgaggcag ggtcttcgac aaactgcgcc cgctgttcgg caacggcatc
    11341 gtcaccaccg acggcgagtt ccaccgcaag cagcgtcgcc tgatgcaacc cgcgttccac
    11401 cgggcccaac tcaccgccta cgcggaggtg atggcccagc aggccgacgc gatggccgcc
    11461 tcgtggactg ccgggcagga gctctcgatc gtgaccgaga tgcggaggtt cgcgctgggc
    11521 tcggtggcca agatgatctt ctcgggtgat atgggcagac ccgccgtggc ggaggtccac
    11581 cgctcgctgc cggtcgtcct ggaaggcatg ctcgtacgag cggtcatgcc caaggcgctc
    11641 gaccggctgc cgatcccgct gaaccgtcgt ttcgatgccg ccgccgcacg gctgcgccgc
    11701 atcatcgacg aggtgatcgt ggagtaccgc gccgagcagg aggaccgcca ggacctcctg
    11761 tcactgctgc tgtccgccgt ggacgccgat acgggcgaga ggatgagcga cgagcaggtg
    11821 cgcgacgagg tgatcgccat catgttcgcc ggcaccgaga cctcggccac ggtgctgtcg
    11881 tggattttcc acgagctggg ccgtgacccc gaggcgcgca agcgactgca ggccgaagtc
    11941 gacggagtag ttggctccgg gcccgtacgc cccgaccacg tcccccagct gacgtacacg
    12001 caccatgtgt tccacgaggc gctgcgcctg cattcgccgc tgctcttcac ccggcgcgcg
    12061 ctggccccgg tgaacctcgc gggcgtggcc attccggccg gagccgaagt ggcctacagc
    12121 ccgtacgcac ttcaccgcga ccccgcgctg ttcccccagc cgcagcggtt ccagccagag
    12181 cgctgggaga agagcccgaa gcgacgaccc cacctgaaca gcttcattcc cttcggggcc
    12241 ggtcagcaca agtgcatcgg ggaccacttc gcggcggcgg agatagtgat cgcggtggcg
    12301 agtgtgatgt cccgatggga cgtggtccac gcgccgggcg tgaccgtgcg cgaggtcgcg
    12361 gcaggcatcc cgcagccgga cgagctgacc atgatgcccg tcgcgcggca gtgacaggcc
    12421 gaccaaggat acggacctga ccggttgtgg agtgcgaagt tccctaaagt ggtccacaag
    12481 cagcaacacc ctgcgcccgt ggccttacac tcggccacac gcgctgaagg ggcgtaggga
    12541 tgacacagga acagtctgcg gaacagccac gatggaccac gccacccgtg ctcgaacggg
    12601 aatccgaact gcacgccatc cggcgtgcgt tggatgacct atgcggcacc tcacccgatg
    12661 gagcgggcac gcgacacggt ggagtgctcg cgttcgcggg cccagcgggg gcgggcaaga
    12721 ccacactgat cgctgaactc cgtcggcagg ccacggaacg cggctgcgcg gtgttgttcg
    12781 cgcgtggcgg cgaacaggaa cgccaggtcc cgttccatgt ggtgcgccaa ctcgtccaac
    12841 cggcactcgc ctccatggac gacagcgagc gccgcgagat cctcgggcac tggtacggca
    12901 tcgtggcacc agccatcggc ctgaccgttt ctcagggcgt cgcggcaccc gacccacagg
    12961 gggtgcgcga tggtctggac tggctggtca cccacttgtc ggtgcgcaac gctcccgtac
    13021 tcctggtact cgacgacgcg cactgggccg atgcggaatc actgacgtgg ctgacagcgt
    13081 tcgcggcgcg ggccgaagag ctggccctgc tcatcgtggt cgcccaccgc ccggaggaac
    13141 tgctcgccga cgtcgctggt ttgcaggcac tggccgagcg ccatggggcg cgcccgcacc
    13201 agctgaccgc cctcaccacg accgcggtgg cgcacctggt gcgtggcgcg ctcggggacg
    13261 acgcggacga caccttctgc cgggagttct gggcgatcac cggaggaaac ccgtttgaag
    13321 tcgtcgagtt gatggcgaag gcgcgtgagc gcgctctgcg gccgacggag ggcagcatcc
    13381 cgcagctgcg ggacctcgcc tccgccgtca agggcaacgg gctcgtggaa cgtctcgaga
    13441 ccctcggtcc ctcaaccgtc cggttggcct gggccgccgc gctgctcggt tcgggtcatt
    13501 ccaccgagct ggtgggcgct gtggccgcgc tcggcgaagc ccagaccgcc gacgccatcg
    13561 agcggctgca cgacgcgcgc atcctgacct cactgcacca catcaggcgt ggcaaggtcg
    13621 tggagttctt ccacccgctg gtcgccaccg ccgtgtaccg ggcgatcccc gcagccgtac
    13681 gcgtcgccat gcacggcatc gcggcgaacg ccctgatcga cgcgggccac ggccccacgg
    13741 ccgccgcccg ccacattctg gagatgcata cggaaggcga cccctgggtc gtacagcaac
    13801 tccgccaggc cgcccgcgac aacttcgcct ctggcgcacc cgacgcggca ctgcgctgtc
    13861 tgacgcgcgc gctgcgggag ccgccagacg tcgaagaccg ggcgcaggtc ctgttcgagc
    13921 tgggttcggc cggtctgttc tacgatcccg cgaccgcggt caaccatctg cgagccgcgc
    13981 tggaggagcc gaaactggac cagggactgc gcgaggccgt cagctaccgg ctggcccagg
    14041 cactcgcgca tgtgggtcag atggggcagg ccacggacct cctggcggaa gaagcgcgcc
    14101 gtacgaccag cgcccgtacc cgactgcgga tgcaggccga gcagttcaag tggaacgcgg
    14161 tccgtgtcga cgaggaggac tccccggccc gctcccggtt gctggcccag ttcgcgaagc
    14221 gactcaccgg ccgcgacctt gccgaacgcc acatcctggg actgcgggcc tgggattcgg
    14281 tgatgcgcgg cgaacccgcg gcccaggccc tgcagttcgc cgagcaggcc ctggacggcg
    14341 gactcagctg gacggaccag gacttcgggt tcgaggtacc ggtcgttgtc gcgctcacct
    14401 tcatgtacgg cgaccagccg ggcagagctg aggagctgtt ccacaagggc attgccgaat
    14461 tcgaggaacg aggctggcgc ggggcacacc tttccttcgc ctacaccctg ctcggctata
    14521 tccgctatcg tcgcggacgg ctcgccgaag cggaggacta tgtccgcaac ggcctgcaga
    14581 tcgccaatcg agtgggacag aacatcccgg cacagtggta cgcgatcggc acgctgatcg
    14641 agacccttat cgcccgtggc gacattgacg aagcccagaa cgtcgccgag gcctacgatt
    14701 tcggaaagga cttcccgaag gccgtcgtct atcccgatcc gcaagccgtc tacggaaagc
    14761 tcctcctcgc ccacggcgcc cgggaccagg ccgaacggca cctcgccgcg gccggccggc
    14821 gcctcgaccg tcgcggcagc cgcaaccccg cctggagccc gtggcagctc gacctcgccc
    14881 tcgcccaggc cgagtacgac cccgaccagg cccgggccac cgcggacgcc gccgtgacgc
    14941 gcgcccgggt gttcggcacc gccagcgcca agggtcaggc gctgcgggtc gcggcggcca
    15001 ccgacccggc ccgggcggcc atgctgctgc acgaagccgt cgagcacctg gaacagtccc
    15061 cggccgccta cgaactcgcc cacgccctca tcgaccacgg cgcggccctg tacgcggaag
    15121 gcaatctgga acacgcctcc cgccagtacc accgcggcct ggaaaccgcc accgcctgtg
    15181 gcgcggacgc cctggccacc cgggcgcgca ccgaactggg cgccgccgga ctacgtcccc
    15241 agcacccgca gaccgcggcg caggacacgc tgaccgaggc ggagagcgcg gcggcacggc
    15301 acgcggtacg cggcctcgac aacgcagcga tcgccgccga actccgcctg gatgagcacg
    15361 ccatatcccg actgctgtcc gcggtcttcg ccaagctggg aacggaccgc acggggctga
    15421 gccgcgccct cggcgggtga ggtctcgctg acgccccgtc ggccgggttc ctgccggtga
    15481 tggtccgttg gtcttgtggt ggcacagtct gactgctcga gactgtgcca atggttcagg
    15541 atgaaagcgg cgagttgagc cagtgggccg cttgatgctt tcctgagtcc atcgatggcg
    15601 ctgcgggtga acccgcggcg cctttgccat gtgaaggacg aaggggtgga ccgcggtcgt
    15661 gcggaagatg ttcgtggctc ctgatccggg gctgctgcgc ctgcgggtct cgctgcgtgc
    15721 cgtgctcggt gtcggactcg cggtggcggc cgccgaagtc gccgggctct cgctgtccgc
    15781 ctcgatcacg ggtggactgg ccgcgctgct cgcgctcttc accgtcggcg atgccaccgt
    15841 acgcgggcag gcggtgacca ccgcactgct gcccgtcgtg ggccttccgg tgctcgccct
    15901 ggccgccgcg ctgcacggga tgccgatggc gcgcgacgcc gccttcgtcg cggtcgtctt
    15961 ctgcggcgtg tacgcgcgcc gctgggggcc gcgcggccac gccctcggca tcttcgcctt
    16021 tatgcagttc ttcgtcgcgc agttcctgca cgccgtcccc gcccagctcc ccgagctcta
    16081 cgccgccgtc gcgctggccc tcgccgccgc ggccgccgtg cgcttcggcg cctggtgcgt
    16141 cgagcggcgc accccgctcc cgatgccgcc cgggccactc gccggagccg ggctggaccg
    16201 ccccagcacc cgacaggcct tccaggcgac cgccgcctgc gccttcgcgg tggtcatcgg
    16261 gcaactggtg tcgggcgacc gctggtactg ggccgtcggc accgcgtggt ggatcttcgt
    16321 caacaccgcg tcccgcggcg agaccctggt gcgtggcttc cgccgcgtac tcggcacggt
    16381 cgtcgggatc gccgccggac tgctgatcgc cgtgccgctg cacggggcgc ccgggcccac
    16441 cgccgcgctc gttgccgtct gcgtcttcgg catcttctac accgcggcac cgtcgtactc
    16501 ctggatgatg ttcttcgtca cgatcatggc gggcctgctg tacggactcc tcggcgtcct
    16561 gcacccgggc ctgctcctgc tgcgcttcgt cgagaccggc gtcggcgcgc ttggtgccgc
    16621 cctcgccgta ctgctggtgc tgccgatcac cacacacgcc gccaccgagg cctggattca
    16681 gcgcgccctg cgcgaggtcc accgctgcac cagcgaggcc gcccggcgcc tggcgggcga
    16741 cgagaacgcc gaccccgcgc cgcacgccgc cgcgctggag gcgctgctcg gccgggtacg
    16801 gatggccctc gcgccgctgg tccacccgct cagcccgctg cgtgcccgca aggcgcgtgc
    16861 ccgccagatc ctcgtactgc tcgacgcgtg tgcccgtgag gtgcgcggtc tggccgtggt
    16921 cgccgccgac cccgacgcct cgcatgacgt ccggctgacc gaggcctgcc accgggtcgc
    16981 cgtagcgctc gaagccctgg tcgatacgga ggaggcgctg gccgagtcct tcgggccgcg
    17041 cccggcgacg gtgccccgtc acgaccctgg cgccgagcag gcgctggctc atctgcggga
    17101 gctggagcag gccctcaccg gcctcgccac gccgttgcgg cgtgagccgc gtccggtact
    17161 cgccaccacc tgaccgtttg gtctagaccg cctgctaccg tctgccggaa actggccgac
    17221 ggaggggttc ggatatgggc ggcatgcggg cgtacatcgg ttccttcacc tctgcgggcg
    17281 gccgtggcat caccacggcc agcatcgacg aggacaccgg agccctcacc gcgctgagcg
    17341 cgacggacgt gctcgccgac ccctcctacc tcgcgttgtc ggacgacggc gaggtgctct
    17401 acgccgtgag tgagagcgag cggggccagg tcgccgcctt cggcgtgcgc gccgagcggc
    17461 cggagccact gggcgcgcca gtcgccgtgg gcggggcgag ccccacccac ctcgcgttgg
    17521 ccgcggggca tctggtcacc gcgaactacg gctcggggag cgtgtcctgg ctgccagtcc
    17581 gcgcgggtgg ccagctcggc gcggcagggg caggggcagg ggtactgcgg cacacgggct
    17641 cgggccccga cccggagcgc cagcgggggc cgcacgcgca ccaggtgctg cccgacccca
    17701 gtggacgctg ggtgctcagc gtcgacctcg gtacggactc cgtacgggtc tgctcgctcg
    17761 tcgcgtcgga ggcggtcgcg gggaaggcgg tcgagggagg ggcagtcacg ggggaggcaa
    17821 taacggggga ggcaggggcg ctcgctgagc tgcggacgca ccgggaggtg gtgctgcggc
    17881 ccggcagcgg accccgccac ctggcgttcc acccgcgcgg cgatctcgcg tacgtggtgc
    17941 atgagctctc cgcgaccgtg acctgctgcc gctgggacgc ccaggcgggg catctggagc
    18001 cgctcggtga gacgcggctg ctgcacggcg gcccggtcgc cggagacgcg tacccgtccg
    18061 gtctggtggt ctccacggac ggccggttcc tgtgggccgc cacacgcggc caggacgtga
    18121 tctccgtgct ggcgctggac ggggacggcg tacggccacg gctcgtcacg acggtggcgt
    18181 ccggcgggca ctggccgcgt gacctcgcgc tgcacccctc ggggcggttt ctgtacgcgg
    18241 cccatgagcg ctcgggcgac gtcacctggt tcgcggtcga cccggagtcg ggtgccctgc
    18301 ggcgggcggg ggcgatcgag gcgcccgcgg cgtcgtgcgt ggtcttcggc tgagagcggg
    18361 cctgacagca gcggcgcggg nnnnnnnnnn nnnnnnnnnn ncccgcgccg ctgtccgtcg
    18421 ccagggaccg atgggtctct ttggtctcgt tgttctcgct actgggcggg cgcgcccgtc
    18481 ggctgcgggg cgatgcccag cgccgtcatg tactgcgaca gcgccagcct gccgatcgcc
    18541 gggtaggcgc ccagggcctc ggcggaggcg cagcccgcgt ccttcgcggc cacttcgagc
    18601 aggccttcgt cgacctcagg gccgatcaca tgcggcgcga gtgcgagctg ggtcgagccc
    18661 gagccgcgca gctgctccgc ggtcgccgcg accgctccct cctggtcgag ggcggcggcc
    18721 atcaccggca ccgcgaggcg cgcggccagc agcatgccgg agatcccggc cgcctgtacg
    18781 gcctcctcgc caccgaccgt ggcgaggatg atgccatcgg ccgccgtcgc gacggtgaac
    18841 agcctggcgc ggtcggcgcg ggccagaccg gcctcggaca gccgtacgtg cagcgcctcg
    18901 gcgagcagcg ggtgcgggcc caggacatcg gtcagctcgg cgggggcgcc actgtccacc
    18961 acggcctgcc ggatctggcc catcagggcg gcgtccggac cggccagcag cggcaccacg
    19021 accgcggccg ggccctcggg ctcggccacc tcgcggccag cggcaagggc cagctcgtag
    19081 cgggcggtgc gctgctcggc ggtctgcgcg agcaccgacc gcagactggg aaactcctcg
    19141 tcgtcaccgt cgaggtagcc gatcctggcg ttcatgccgg gcagctcgga gcgggcgatg
    19201 ctcacgacct cctcggcgag actccgggtg gccgtgctgg gtacgcccgg cacggcgagg
    19261 acgagagcgg gtgctccctc gggagccacc gcgggttccg gacggcggtg ccgaccgggc
    19321 tgtcggggtc gcggcattcg tacaggcagg ccggaagcgg gcccagtggg ggagctcatg
    19381 gcgccgcatg ctactggctt cgtgggttcc cccgtttggg gagggtccag gtgcgaggtt
    19441 tccgtccgga aatttccgtt gtgtgttgta agggtcaact gtcggtttcc tttgggatgt
    19501 acaagaggtg tggatcgggc ggcagcgccg ttccgccgcc cgcgaggtcg agcgcgatgc
    19561 gtacggctcc atccagcgga tcgcccgcgg cgggcaccag ccgggcgtgc ggcagttgct
    19621 cggcgagctc ggcgcgcagg ggtacgagca ggggttcgcc caggcggaac aggcctccgg
    19681 tgaaggccac ttcagggggc tcgtcgctgc cgtccggagc gggatccgga gcgggatccg
    19741 gagcgaggac gggagcgggg gcgggacata cggccgctgc cgcctcggcg atgtgccggg
    19801 cggcggcggc cagaatcccg gcggcgaccc ggtcgtgtgc cgcgcactcg gcgacgctgg
    19861 gcgcgaagga ggccagtacg gcgggccggt cgccgcgcgg gtagagctgc ccgggcagct
    19921 cggacaccgg accgaacgtc tcgcgggcgc gggccagcag cgcccgcgaa cccccgcgcc
    19981 gcccgtcgta tgcccgcagc gccgcctcga gtcccgcccg gccgatccag gcaccgctgc
    20041 cgcagtcgcc gagcagatga ccccagccgt cggctcgccg ccaccgggtg agatcggtcc
    20101 ccagcgcgac catgccggtg cccgcggcga cgaccgcgcc ggggcgctgc ccgagcgccc
    20161 ccgcgtacgc ggtcacggcg tcggcggcca gcgcgaggcg acgtacgcca agcgtggccg
    20221 tcagcgccga cggcagtttg gcgcgcagct ccccgccgag cgtggccatc cccgcggctc
    20281 cgatgacgac ggcctcaacc accgtgcctc cgccgccccc ggcactttcg atcagcccgg
    20341 cggccatcgg cgtcagctgc tccagcagat gatccgcgtc gatgccaccg gaacccgtgc
    20401 gcaccggctc acgcgacatg accggctcca cctgccgcaa cccgcccccg gtgacaccca
    20461 gcgcgacccg cagccccgag ccccccgaat ccacccccag cacgcaggac ggtccaccgt
    20521 cgccctcggg cgtgtgcctg cgcgacctca tggagcggct ccagggagag gaacggaaag
    20581 cggaggtgtg acgacggcgg gcagcctacc gacaggctcc caggtccgca tgattgtcgg
    20641 agcgggttcc tgggccggta gggtgatgtg ccgtggcagc acgaccgttg aatgaacttg
    20701 tagagcccgg ctgggcccag gcgttgggcc cggtggcggg gcgggtcgcc gcgatgggcg
    20761 acttcctgcg tgcggagatc gcggctggac gcacctacct tcctgccggg gcgcacgttc
    20821 tgcgcgcctt ccagcaacct tttgacgagg tacgcgtcct gatcgtgggt caggacccct
    20881 accccacccc gggaatggcc atcgggctca gctttgcggt cgcgcctgag gtgcggtccc
    20941 tgccggggag cctggagaac atcttccggg agatgcacac cgacctgggc cacccacgcc
    21001 cctccaacgg cgacttgacg ccctggaccc agcagggcgt gctcctg
//
