LOCUS       NZ_WHOM01000045        10405 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 471, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000045
VERSION     NZ_WHOM01000045.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 359982)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 359982)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 262555
            Orig. end                         :: 272960
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(575..1834)
                     /locus_tag="GCN81_RS11235"
     CDS             complement(575..1834)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983501.1"
                     /locus_tag="GCN81_RS11235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_231626633.1"
                     /sec_met_domain="p450 (E-value: 1.4e-14, bitscore: 48.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDDGGVDMDMDVDSGVDEALESLRRDPYPLYAQARAAEGLTFVP
                     ELDAWLVSRYADVREVLRRPEDFSSANALRAEVTPSPEALAVLGTGPGSGPVVVTSDGT
                     AHQRLRAPLLRGLSAARVAEVRGFITKRVTTLVDGFAADGRAELMADFAARLPGEVICH
                     LLGLAPDETAVAVHGSRRSEDLFFHPLPVDEQVRAARDMVAARELLAAHAVRRWAHPGT
                     DLTSEMVRSLAPEGPEPSGERLGEIVSNLYNLLIAGHLTTTALIGTALHRLLSPRARWE
                     ALCAAPDAVPAVVAEALRWGSPVQGFRRVTTRPVTLAGTELPEGAPLFVSYAAANRDPA
                     LTTDPDTFDAARGPTRHVAFGHGVHGCPGSGLAREELRIALTVLTRRLPSLRLAPGARV
                     DMLPTLIHWSPRALPVVWTA"
     gene            1976..2713
                     /locus_tag="GCN81_RS11240"
     CDS             1976..2713
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518879.1"
                     /locus_tag="GCN81_RS11240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_156724267.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEQGLAAFAHGDQVEVVHRSFELDP
                     GRAKGDTAPVIDMLAAKYGRTREEARAMEEHVASNARAEGLGYRTEGRDHGNTFDIHRL
                     LHLARDRGRQDELLNLAYRANFAEERSVFDPEVLLALAVEAGLDEGEVRAVLADGDAYA
                     DQVRADEREAAELGATGVPFFVLDRRFGVSGGQPAEVFTQALEQAWGSRSPLQPVGAAS
                     AESGEGEACGPDGCAVPQVKGDV"
     gene            2747..3823
                     /locus_tag="GCN81_RS11245"
     CDS             2747..3823
                     /NRPS_PKS="Domain: Aminotran_5 (25-352). E-value: 9.3e-21.
                     Score: 66.1. Matches aSDomain:
                     nrpspksdomains_GCN81_RS11245_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143405.1"
                     /locus_tag="GCN81_RS11245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_156724269.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.1e-17, bitscore:
                     55.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGELAVPQSGGMESLAGGAFAPRNTYLNTASTGLLPEPAAVAMR
                     QAIEGAAVGRPTDMFEDVEASRAAFARLAGVPASRVAAGSSVSVYTGMIAASLPQGAEV
                     LVADGDFSSLVNPFHMRRDLSVRTVALEKLADAVRPETALVACSAAQSADGRVAGLPAI
                     RAAARAHGARTLVDASQSAGWLPLDATADDYTVAVAFKWLFCPRGIVFFVVPEDLGGLN
                     AVFAGWVAGERPWDSCYGPVAELASSARRFDISPSLFSYPGARASLALVEELGVDAIHA
                     HDLALAGRFRDGLGALGHTPVPAPGSAIVSVPGLGHRQPELSRADIEVSDRAGNLRASF
                     HLYNTAADVDRLLDVLSA"
     aSDomain        2822..3802
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS11245_Aminotran_5.1"
                     /evalue="9.30E-21"
                     /label="GCN81_RS11245_Aminotran_5.1"
                     /locus_tag="GCN81_RS11245"
                     /protein_end="352"
                     /protein_start="25"
                     /score="66.1"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPEPAAVAMRQAIEGAAVGRPTDMFEDVEASRAAF
                     ARLAGVPASRVAAGSSVSVYTGMIAASLPQGAEVLVADGDFSSLVNPFHMRRDLSVRTV
                     ALEKLADAVRPETALVACSAAQSADGRVAGLPAIRAAARAHGARTLVDASQSAGWLPLD
                     ATADDYTVAVAFKWLFCPRGIVFFVVPEDLGGLNAVFAGWVAGERPWDSCYGPVAELAS
                     SARRFDISPSLFSYPGARASLALVEELGVDAIHAHDLALAGRFRDGLGALGHTPVPAPG
                     SAIVSVPGLGHRQPELSRADIEVSDRAGNLRASFHLYNTAADVDRL"
     gene            complement(3945..4844)
                     /gene="thpD"
                     /locus_tag="GCN81_RS11250"
     CDS             complement(3945..4844)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="GCN81_RS11250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_156724270.1"
                     /sec_met_domain="PhyH (E-value: 6.7e-39, bitscore: 128.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVLIDREDPVVWSEPGTQGPISANELA
                     GFERDGFLAIDQLIAPDEVEVYHAELERLMNDPAMRADERSIVEPRSQDIRTLFEVHKI
                     SELFGRLAADPRVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGIP
                     NMRTVSVSIALTKNYDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEA
                     LTQLASRYGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNFFLVFNSVENAAVEPFS
                     APIRRPEYIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="GCN81_RS11255"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536875.1"
                     /locus_tag="GCN81_RS11255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_156724272.1"
                     /sec_met_domain="ectoine_synt (E-value: 2e-54, bitscore:
                     177.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTDDTTGEKYIITPGTMYLLDGHEKHTMRIKQDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPS"
     gene            complement(5483..6751)
                     /gene="ectB"
                     /locus_tag="GCN81_RS11260"
     CDS             complement(5483..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 3.3e-74.
                     Score: 241.8. Matches aSDomain:
                     nrpspksdomains_GCN81_RS11260_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="GCN81_RS11260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_156724273.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2e-79, bitscore:
                     261.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNTILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGRTPDFIWFESLLENSGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRQLADVCKRWDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEAALS
                     AIRAEHPEATKEYRGRGLVWGLEFHDKDRAGKVAKRAFELGLLIETSGPESEVVKMLPA
                     LTITADELDEGLRTLARAVRETA"
     aSDomain        complement(5657..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS11260_Aminotran_3.1"
                     /evalue="3.30E-74"
                     /label="GCN81_RS11260_Aminotran_3.1"
                     /locus_tag="GCN81_RS11260"
                     /protein_end="365"
                     /protein_start="28"
                     /score="241.8"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNTILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGRTPDFIWFES
                     LLENSGSGLNKPAAVIVETVQGEGGINVARAEWLRQLADVCKRWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEAALSAIRAEHPEATKEYRGRGLVWGLEFH"
     gene            complement(6850..7407)
                     /gene="ectA"
                     /locus_tag="GCN81_RS11265"
     CDS             complement(6850..7407)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="GCN81_RS11265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_156724275.1"
                     /transl_table=11
                     /translation="MTAAQADLAPTRSDIAEKLGDLRLDRPQVEDGAALWRIARDSKTL
                     DLNSSYSYLLWCRDFADTSVVARDSGGDPVGFISGYLRPDRPDTLLIWQVAVDAAQRGR
                     GLAAAMLDSLSASVAKESESGLSTLETTITPGNTASERLFASYAERHGASVETEVLFDA
                     GLFPDGGGHDPEVLYRIGPLSF"
     misc_feature    7360..7370
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    7573..7588
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7573..7590)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     gene            complement(7785..8933)
                     /locus_tag="GCN81_RS11270"
     CDS             complement(7785..8933)
                     /NRPS_PKS="Domain: Aminotran_5 (15-263). E-value: 7.7e-30.
                     Score: 95.9. Matches aSDomain:
                     nrpspksdomains_GCN81_RS11270_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238578.1"
                     /locus_tag="GCN81_RS11270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_156724276.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.3e-25, bitscore:
                     83.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFAAIERRVARLLGTEQDVVIMQGEALLPLE
                     GCIRSGAKPGSTALNVVTGPYGQTFGDWLRDCGAHVVDLAVPFHTAVTARQVEQALTEH
                     PEIDFVSLVHAEAATGNTNPVAEIGKVVRAHGALFMVDAVASVAAEPLLVDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRFTENPGAPRRSYLSLLDWKERWIDGGRRALPHAP
                     AQLEMLALEACVERIEAAGLDAVTARHARAAEATRAGALALGGGRSGRPGLSGPSALEP
                     YVHDAADAAPVATTLRAPAGTDASELVARALAADPTLPLVAGGGALAHEMIRVNHYGPD
                     ATHDVVHATLAALGAALGDMSGPAVDLEAASRAVTAVWTRPA"
     aSDomain        complement(8145..8888)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS11270_Aminotran_5.1"
                     /evalue="7.70E-30"
                     /label="GCN81_RS11270_Aminotran_5.1"
                     /locus_tag="GCN81_RS11270"
                     /protein_end="263"
                     /protein_start="15"
                     /score="95.9"
                     /tool="antismash"
                     /translation="FAAIERRVARLLGTEQDVVIMQGEALLPLEGCIRSGAKPGSTALN
                     VVTGPYGQTFGDWLRDCGAHVVDLAVPFHTAVTARQVEQALTEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGKVVRAHGALFMVDAVASVAAEPLLVDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWQRFTENPGAPRRSYLSLLDWKERWIDGGRRALPHAPAQLEMLALEACVERI
                     EAAGLDAVTARHARAAEATRAGALAL"
     gene            9014..10105
                     /locus_tag="GCN81_RS11275"
     CDS             9014..10105
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989359.1"
                     /locus_tag="GCN81_RS11275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_156724278.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.6e-18, bitscore:
                     61.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDQAVLRVTGRVLVGPDDVRDELWVVGGRVSYTRPAGVRDVRTV
                     QGWALPGLVDAHCHVGLDAHGAVPEDVAEKQALTDREAGTLLIRDAGSPSDTRWIDDRA
                     DLPKIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAQEARRGDGWVKLVGDWLDRGSGD
                     LSACWPRGAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPKLAESGDAKFPIWADHMRRLYERRYDTVRAAYDAGVPIFVG
                     TDAGGSLPHGLAAAEVTELTKAGIPPLRALSATTWAARAWLGRPGLDEGAPADLVVYDE
                     DPRTDVRVLAAPRRIVLNGTVVG"
ORIGIN
        1 ggcctgggcg aaggcagccc cgagaccgac gcgcactacc gaccggtacg gcgctcgctg
       61 gacgcggcgc tgtccgccgt cggtgccacg gccgaggaca tcgccctggt catcaacagc
      121 catctgcact tcgatcactg cggcggaaac ccggcactgg tcggttgccc ggtcctggtc
      181 caggccacgg agctggcggc ggcgcgggcc ggggactaca cgatcgacgc gctggtgacc
      241 ttccccggcg cggtgtacga gcagctcgac ggggaggcgg agctctggcc gggcgtctgg
      301 gtggtgccga cccccggcca tacggccggt caccagtccc tggtcgtcga gggcagcggc
      361 ggggccacgc tcctggcggg ccaggcctac gacttcgcca gcgagtacgc ctgcgcggac
      421 ctggcccggc gtacgggcgt gcccgccgcc gaaggcctgt ggcgcccctg gctggccagg
      481 atggccgaac tgggcgtcga ccgggcgctg ttcgcccatg accacgcggt atggaccagc
      541 tgaaccggaa ccagggcgtc tggtccgtag gcccctaagc cgtccacacc accggcagcg
      601 cgcgcggtga ccagtggatc agcgtgggca gcatgtccac ccgcgctccc ggcgcgagcc
      661 gcagtgacgg cagtcggcgg gtcagcaccg tcagcgcgat ccgcagctcc tcgcgggcga
      721 gccccgagcc cgggcagccg tgtacgccat ggccgaaagc gacgtgccgg gtggggccgc
      781 gggcggcgtc gaaggtgtcc gggtccgtgg tcagggccgg gtcccggttg gcggcggcgt
      841 aggacacgaa cagcggggct ccttccggca gttcggtgcc cgccagcgtc accggacggg
      901 tggtgacccg tcggaaaccc tgcacggggg agccccaccg gagtgcctcg gcgaccaccg
      961 ccgggaccgc gtcaggggcg gcgcacaggg cctcccaccg ggcacgcggc gacagcaacc
     1021 ggtgcagcgc ggtgccgatc agcgccgtgg tggtcagatg cccggcgatc agcaggttgt
     1081 acaggttcga cacgatctcc ccgagccgtt ccccggacgg ttcggggcct tcgggtgcca
     1141 gggaccgcac catctccgag gtgagatcgg taccggggtg tgcccagcgg cgtaccgcgt
     1201 gcgcggcgag cagctcccgc gccgccacca tgtcccgtgc ggcccgcacc tgttcgtcga
     1261 ccggcagtgg atggaagaac aggtcctccg agcgccgact gccgtgcacg gcgaccgctg
     1321 tctcatcggg ggccagaccg agcaggtggc agatcacctc gccgggcagc cgcgccgcga
     1381 agtcggccat cagctcggcc cgcccgtcgg ccgcgaagcc gtccaccagc gtcgtcaccc
     1441 gcttggtgat gaacccgcgt acctcggcga cccgcgccgc gctcagcccg cgcagtagcg
     1501 gggcgcgcag ccgctggtgc gcggtgccat ccgaggtgac gaccacgggg ccgctaccgg
     1561 gacccgtgcc aagcacggcc agcgcctcgg gtgacggggt cacctcggcg cgcagcgcgt
     1621 tggccgacga gaagtcctcg ggcctgcgca gcacctcgcg gacatccgcg taccggctca
     1681 ccagccaggc gtccagctcc ggtacgaagg tgagcccttc ggcggcgcgg gcctgcgcgt
     1741 agagcgggta ggggtcgcgc cgcagcgatt caagcgcctc gtccactccg ctgtccacgt
     1801 ccatgtccat gtccacgccc ccgtcgtcgg tcatcggccc atcgtgcagc agcggtgcgc
     1861 gacaggacag gacgagatcc ggccaccgcc ccagggaatg tgccgatccc ctccctcgtt
     1921 gagcagggag cggagggtgc ctccgtaccc gtacgaccgg agagaagtca tacagatgcg
     1981 cgtcgagatc tggagcgata tcgcctgccc gtggtgctac atcggcaagg cccgtttcga
     2041 gcagggcctg gcggccttcg cccacggtga ccaggtcgag gtggtgcacc gctccttcga
     2101 gctcgacccg ggccgtgcca agggggacac cgcgccggtg atcgacatgc tggcggcgaa
     2161 gtacgggcgc acccgtgaag aggcgcgggc gatggaggag catgtcgcct ccaacgcgcg
     2221 cgccgaggga ctcggctacc gcaccgaggg ccgcgaccac ggcaacacct tcgacatcca
     2281 ccggctgctg cacctggcca gggaccgtgg ccgccaggac gagctgctga acctggccta
     2341 ccgggcgaac ttcgccgagg agcgctccgt cttcgacccc gaggtgctgc tggcgctcgc
     2401 cgtcgaggcc ggtcttgacg agggcgaggt ccgcgcggtg ctggccgacg gtgacgcgta
     2461 cgcggaccag gtgcgcgcgg acgagcggga ggccgccgag ctgggcgcga ccggggtgcc
     2521 gttcttcgtg ctcgaccggc gcttcggtgt ctccggcggg cagccggcgg aggtcttcac
     2581 acaggccctg gagcaggcgt gggggagccg ctcgccgctc cagccggtgg gcgcggcgag
     2641 tgctgagagc ggcgagggcg aggcctgtgg gcctgatgga tgcgcggttc cgcaggtcaa
     2701 gggcgacgta taagcatcac ttagaggtta gcgtcaactt tatgcaatgg acggggagtt
     2761 ggcggtgccg cagagtgggg gcatggagag cctcgcaggc ggcgcgttcg cgccgcggaa
     2821 cacgtatctg aacaccgcca gcaccgggct gctgcctgag cccgccgccg tcgcgatgcg
     2881 gcaggccatc gagggcgcgg ccgtcgggcg cccgaccgac atgttcgagg atgtggaggc
     2941 gagccgggcg gcgttcgccc ggctcgccgg cgtcccggcg agccgggtcg cggccgggtc
     3001 ctcggtctcc gtctacaccg ggatgatcgc cgcctcgctg ccccagggcg ccgaggtgct
     3061 ggtcgccgac ggtgacttca gctccctggt gaaccccttc catatgcgcc gcgacctctc
     3121 ggtgcgcacg gtggcgctgg agaagctcgc cgacgcggtg cgcccggaga ccgccctggt
     3181 cgcgtgcagc gccgcgcagt ccgcggacgg ccgggtcgcc ggcctccccg cgatccgggc
     3241 cgccgcgcgc gcccatggcg cccgtacgct ggtcgacgcc tcgcagtcgg cgggctggct
     3301 gccgctggac gcgacggccg acgactacac cgtggcggtg gccttcaagt ggctgttctg
     3361 tccgcgtggc atcgtgttct tcgtcgtccc ggaggacctc ggcgggctga acgccgtctt
     3421 cgcgggctgg gtcgcgggcg agcggccgtg ggacagctgc tacgggccgg tggcggagct
     3481 cgcctcctcc gcccggcgct tcgacatcag ccccagcctc ttctcgtacc ccggtgcccg
     3541 cgcctcgctc gcgctggtcg aggagctggg tgtggacgcc atccacgcgc atgacctggc
     3601 gctcgccggt cgcttccgcg acggccttgg cgccctgggg cacactccgg tgcccgcgcc
     3661 gggttcggcg atcgtgtcgg tgccgggcct ggggcaccgg cagccggagc tgagccgggc
     3721 cgacatcgag gtctcggacc gcgccgggaa cctgcgcgcc tccttccacc tgtacaacac
     3781 cgcggccgac gtggaccggc tgctcgacgt gctgtccgcc tgacgaggtc cgcctgacga
     3841 ggtccggttg acgagagctg gctgacgagg tctggtggac gcaatgcggc ccggcacccc
     3901 ggaacggcgg ggtgccgggc cgcatcgcac tacctcacga gcccttacct cacgggcgtg
     3961 aagtcccgcg ccccgatgta ctcggggcgc cggatcggcg ccgagaaggg ctctaccgcg
     4021 gcgttctcca cgctgttgaa cacgaggaag aagttgctcc gcgggtaggg agtgatgttg
     4081 tcccccgaac cgtgcatggc gttgcagtcg aaccaggtgg ccgaacccgc ctcgccggtg
     4141 aacagccgga ttccgtaccg cgaggcgagc tgggtgagcg cctcgtccga cggggttccg
     4201 gcatcctgca tctgcagtga cttcttgtag ttgtccttcg gcgtcgcgcc cgcgcagccc
     4261 aggaacgtac gatgcgaccc cggcatgatc atcagaccgc cgttggtgtc gtagttcttg
     4321 gtgagcgcga tggacaccga gacggtccgc atgttcggga tgccgtcctc ggcgtgccag
     4381 gtctcgaagt ccgagtgcca gtagaagccg gtggcgccga agcccggctt cacgttgatg
     4441 cgcgactggt gcacgtagac gtcggagccc aggatctgac gggcccggcc gacgacgcgc
     4501 gggtcagcgg cgagcctgcc gaagagctcg ctgatcttgt gcacctcgaa gagggtgcgg
     4561 atgtcctggg agcgcggctc gacgatggag cgttcgtccg cccgcatcgc gggatcgttc
     4621 atgagccgtt ccagctcggc gtggtagacc tctacctcgt ccggagcgat cagctggtcg
     4681 atcgccagga aaccgtcccg ttcgaacccc gccagctcgt tggccgagat cggcccttgc
     4741 gtacccggct cggaccacac cacagggtcc tcgcggtcga tgagcacctc ggtggcgcct
     4801 cgggtcgggt acaggtcggc ggtgcgttcg ggtgcggtgg tcatggtgtt gcctgcctct
     4861 cctctcgtac ggcccttacg ggatgtacac ctcggccgcc ccccggtaag agcaggggcg
     4921 gccgaggaga tcagggggcg tcaaagcccg ggcgtaagcc cgctggtcag ctgtcagctg
     4981 gttgagccgg ttgacctggt tcagctgggg tcaggctccg tgagcagcgg gtacacgccg
     5041 ttctcgtcgt ggtcctctcg gcccgtcacc ggcgggttga agacgcacag gcagcggaag
     5101 tcctgcttga tccgcatcgt gtgcttctcg tgcccgtcca gcaagtacat cgtgcccggc
     5161 gtgatgatgt atttctcgcc ggtcgtgtca tcggtgagtt cggcctcgcc ctcgacacag
     5221 acgacggcct cgatgtggtt ggcgtaccac atggacgtct cggtgcccgc gtacaggacg
     5281 gtctcgtgca gcgagaagcc gacgcgctcc ttggcgagca cgatccgctt gctctcccag
     5341 gtgccggagg ccgacttgac gtggcggtcg gtgccttcta tgtccttgaa cgaacggacg
     5401 atcacggtga gtcgattcct tgtctcttac gggtacgggg gctacgggta cgcgggcggt
     5461 acggggaccg ggtgccgcgg ggtcaggcgg tctcgcgcac ggcgcgggcg agggtacgca
     5521 gtccctcgtc cagctcgtcg gcggtgatcg tcagcgcggg gagcatcttg acgacctcgc
     5581 tctccggacc ggaggtctcg atcagcaggc ccagctcgaa ggcccgcttg gcgaccttgc
     5641 ccgcgcggtc cttgtcgtgg aactccaggc cccagaccag accgcggccg cggtactcct
     5701 tggtcgcctc ggggtgctcg gcgcggatcg cggacagggc ggcctcgacc tgctcgccgc
     5761 gggccagggt ctgcttctcc atctgcccgt cggcccagta cgcgtccagc gcggcggccg
     5821 ccgtgacgaa ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga
     5881 tgtccagctc gggcttgaac agggtcagcg ccatcggcag accgtagccg ctgatggact
     5941 tcgagacggt gacgatgtcc ggcgtgatgc ccgcctcctc gaaggagaag aacgcgccgg
     6001 tgcggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcccag cgcttgcaga
     6061 cgtcggcgag ctgacgcagc cactcggcgc gcgcgacgtt gatgccgccc tcgccctgca
     6121 cggtctcgac gatcacggcc gcgggcttgt tgagcccgga accggagttc tccagcaggc
     6181 tctcgaacca gatgaagtcc ggggtccggc cgtcgaggta gttgtcgaac ggcatcggcg
     6241 tgccgtgcac cagcgggata ccggcaccgg cgcgcttgaa ggcgttgccg gtcacggcga
     6301 gtgagccgag cgacatgccg tggaaggcgt tggtgaagga cacgatcgac tcgcggccct
     6361 tgaccttacg ggccagcttc agcgccgact cgacggcgtt ggtgcccgtc gggcccggga
     6421 acatgacctt gtacggcagg tcacgcgggc gcaggatcgt gttctggaac gactccagga
     6481 acgcgcgctt cgccgtggtc gacatgtcga gaccgtgggt gacgccgtcc cgctcgatgt
     6541 agtcgatcag ggcgcgtttg aggacggggt tgttgtggcc gtagttcagc gaaccggccc
     6601 cggcgaagaa gtcgaggtac tcgtggccgt cctcgtcgta catgcggctg ccctgcgcgc
     6661 ggtcgaagac ggtgggccaa ccgcggcagt agctgcgcac ctccgactcc agggtctcga
     6721 agacgctcag gtcgggctgg gtgatggtca cagcgaatct cctcctggga gatgaaagac
     6781 gtgggggggt gttacctgga catggggtct gtcggaaagc tcgggggcgg gccgtcgtgg
     6841 ccctgcggct cagaaggaca gggggcctat ccggtagagc acctcggggt cgtgcccgcc
     6901 gccgtcgggg aacaggcccg catcgaacag cacctcggtc tcgacggagg cgccgtgccg
     6961 ctcggcgtac gaggcgaaca ggcgctcgga ggcggtgttg cccggggtga tggtggtctc
     7021 cagcgtgctc agcccggact cggactcctt ggcgacgctc gcggacaggc tgtccagcat
     7081 cgccgcggcc aggccgcgtc cgcgctgtgc cgcgtccacg gcgacctgcc agatcagcag
     7141 ggtgtccggg cggtcggggc ggaggtatcc ggagatgaag ccgactgggt ctccgccgct
     7201 gtcgcgggct actacggagg tgtcggcgaa gtcacggcac cacagcaggt agctgtagga
     7261 ggagttgagg tcgagggtct tggagtcgcg cgctatgcgc cagagcgcgg ctccatcctc
     7321 cacctgaggg cggtcgagcc gcagatctcc cagcttttcg gcaatgtcac tacgggtcgg
     7381 tgctaggtct gcttgtgcag cggtcatacg tattgaattt accgacctaa aatcaaaatt
     7441 gcatcgcgag aaggggttgt gtgagggtcg cgtatgtggt atcacgcggg ggtgcgtgct
     7501 cgcgtgtacg cttggcgtga tgtggtgatt tgcccgggat cattggggtg aaccggacag
     7561 cctgttgatt aggtcacaga gccgtaactc tcgcgagatc tgtccgactt acgaggtttg
     7621 gcgttcgcga aatcttagtg tttagcctcg aagaaaccgg gcagacgaat acgggaagct
     7681 gcgctgaaaa agcagctccc ggtattcgtg gaatgctagc taattcgtgg ggctcagtac
     7741 cgtcaatgct ttgcctgagg cccgtaattc ggctacggcc gcggctacgc gggccgggtc
     7801 cagaccgccg tgaccgcccg gctggccgcc tccaggtcca cggcgggccc cgacatgtcg
     7861 ccgagcgcgg cgcccagggc cgccagtgtg gcgtgcacca cgtcgtgggt ggcgtcgggg
     7921 ccgtagtgat tgacccggat catctcgtgg gcgagcgcgc cgccaccggc caccaggggc
     7981 agcgtcgggt cggccgccag cgcccgggcc accagctcgg acgcgtcggt tccggctggg
     8041 gcgcgcaggg tcgtggcgac cggtgccgcg tccgccgcgt cgtggacgta cggctccagg
     8101 gcgctcgggc cactcaggcc gggccgtccg ctccgtccgc cgcccagcgc gagcgcgccc
     8161 gcgcgggtgg cctcggcggc ccgcgcgtgc cgcgccgtca ccgcgtccag gcccgccgcc
     8221 tcgatgcgct ccacacacgc ctcgagggcg agcatctcca gctgcgccgg agcatgcggc
     8281 agcgcgcggc ggccgccgtc gatccagcgc tccttccagt cgagcagcga caggtacgag
     8341 cggcgtgggg ccccggggtt ctccgtgaac cgctgccagg cgcgctcgct caccgagacc
     8401 gcggagacgc ccgcgggccc gcccatcgcc ttctgcgcgc cgatcacgca cagatccacg
     8461 ccccaggcgt cgaccagcag cggctcggcg gccaccgagg cgaccgcgtc gaccatgaac
     8521 agggcgccgt gggcgcgtac caccttgccg atctcggcga ccgggttggt gttgccggtc
     8581 gccgcctcgg cgtgcaccag cgaaacgaag tcgatctccg gatgctcggt cagggcctgc
     8641 tcgacctggc gcgcggtcac cgccgtgtgg aacggcacgg ccaggtccac gacgtgcgct
     8701 ccgcagtcgc gcagccagtc gccgaaggtc tgcccgtacg gcccggtgac cacgttgagc
     8761 gcggtcgagc cgggcttcgc gccggacctg atgcagccct ccagcggcag cagcgcctca
     8821 ccctgcatga tcacgacgtc ctgctcggtg ccgagcagcc gcgccacccg ccgctcgatc
     8881 gcggcgaagt gcgcggcgct cagcggggcg aggtcgagga aggggtgggt cacggtgcag
     8941 ctctcttcgt ttcgttcgcg aggtcgtccg gggctgtcgg tcgagcgtac ccagggcgtt
     9001 ctacgctgcg ggtatgacgg atcaagcggt gctgcgggtg acggggcggg tgctggtcgg
     9061 ccccgacgac gtacgggatg agctgtgggt cgtcgggggg cgggtctcgt acacccggcc
     9121 agccggggtg cgcgatgtgc gcaccgtcca gggctgggcg ctgccgggac tggtggacgc
     9181 gcactgccac gtcggtctgg acgcccatgg cgccgtgccc gaggacgtcg ccgagaagca
     9241 ggcgctcacc gaccgggagg cggggacgct gctcatccgg gacgcggggt caccgtccga
     9301 cacccgctgg atcgacgacc gcgcggacct gccgaagatc atccgcgccg ggcggcatat
     9361 cgcccgcacc cggcgctaca tccgcaacta cgcgcacgag atcgagcccg ccgacctggt
     9421 cgcctacgtg gcccaggagg ccaggcgcgg cgacggctgg gtcaagctcg tcggtgactg
     9481 gctcgaccgt ggcagcggcg atctgtccgc ctgctggccg cgcggggctg tcgaggccgc
     9541 gatcgccgag gcgcaccggc tcggcgcccg ggtcaccgcg cactgcttcg ccgaggactc
     9601 gctgcgcgac ctcgtcgagg ccggaatcga ctgcatcgag catgccaccg gcctgaccga
     9661 ggacaccatc ccgcttttcg ccgagcgtgg tgtcgccatc gtcccgaccc tggtgaacat
     9721 cgcgaccttc ccgaagctgg ccgagtccgg ggacgccaag ttcccgatct gggccgatca
     9781 catgcggcgg ctgtacgagc gccggtacga caccgtccgc gccgcctacg acgccggggt
     9841 gccgatcttc gtcggcaccg acgcgggcgg ctcgctcccg cacggcctgg cggcggcgga
     9901 ggtcaccgag ctgaccaagg cgggcatccc gccgctgcgc gcactgtccg cgacgacctg
     9961 ggccgcccgc gcctggctcg gccgcccggg tctcgacgag ggcgcccccg ctgacctcgt
    10021 cgtctacgac gaggacccac gcaccgatgt ccgggtactc gcggcacccc gccgcatcgt
    10081 cctgaacgga acggtcgtcg gctgagagcc ggtccttcct atccttccta gcgcggccac
    10141 atcggcggat tggtgcagaa gtggccgccc aggtgggcgt gttcggggtt gtccgggtcc
    10201 agttcgccgt gctcggccag cagcttcgcg gcgtacggtt ccgagtcgtc ctgcgggtcg
    10261 tagccgaggg cccgtgcgga ggcgaggtcc cacggcaggc gggtgttggc cgacgagccg
    10321 tacaccacgg tgtggccgac gccgctcgcg gtcagtgccg cgtggaagag gcgggcgcag
    10381 tcggccgggc tcatccagat cgaga
//
