LOCUS       NZ_WHOM01000036        10362 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 452, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000036
VERSION     NZ_WHOM01000036.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 493755)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 493755)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10362
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10362
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10362
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10362
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3982:5362](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3983..5362)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(<1..871)
                     /locus_tag="GCN81_RS03720"
     CDS             complement(<1..871)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964396.1"
                     /locus_tag="GCN81_RS03720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="WP_267904550.1"
                     /transl_table=11
                     /translation="MNGPSDNDTEAEAGREPEAGALRGLLPALSRHRVMVVRTCVAAFV
                     DQAALVALVTLLAHTVGAAVVDQVVPSAATVAVLALLVLVRAVATWREMDLSHDLAYRV
                     LAELRVRVFDGLARSAPARIGGLRSGDLAATALADVEALEFFYAHTTAQLLASGAVFGA
                     GGVVLAGAEPWLLAAVLPAALLLALAPLIGARERARRGARTRAAAAKLSADAVEAVDGL
                     RELLAFGALDQRRTELAEQGEELGRAQRAEQEWEATAAAARDVVVVAAVLGVVAVVAHA
                     VAGGRLDGA"
     gene            complement(868..2610)
                     /locus_tag="GCN81_RS03725"
     CDS             complement(868..2610)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="GCN81_RS03725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_156721993.1"
                     /transl_table=11
                     /translation="MMLHRELLTAARTVRGPLTAATALLGAVAATHLAQAVLLALALAA
                     LARGATGELPALLGAVLAVVATRFWLGRRQRRVAVGAGALARVRLRDALLERLGQLGPA
                     HLTTARAGAVRATLVDGVEGVDAYLSRYLPQLAITCLVPPLLLVPLGVLQPYVLLALVP
                     ALALALLAPRWWDRLLARRGKEHWDSYEGLAADYLEALQGMPSLRAAGAVGRTRESLER
                     RSAALHRATVAKLRVSLVDTGLTDLAVQGGTVAAALLAAVAAGGGHAAPVSTYLLLMLA
                     SECFRPIRDLSREWHAGYLGVSAADGIAALRTARPDIADSGRRPADWKAPPELRFDDVR
                     FTYPDAARPALDGVSFTAEGGRTTAIVGPSGAGKSTLLALLLRGRESQRGEVTVDGEPV
                     ASYGLDALRRHIAVVSQETYLFHDTIAANLRLARPGASDDELTAAARAAGVHDEIAAFP
                     DGYGTVLGERGATLSGGQRQRLALARALLADAPVLVLDEATSAVDERREARIGRELLRA
                     ASGRTCIVVAHRLESVRHADRIVVLNGGRVDAIGDHTSLLSDEGLYARLVRAGHEANEG
                     DRAA"
     gene            complement(2607..3974)
                     /locus_tag="GCN81_RS03730"
     CDS             complement(2607..3974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508299.1"
                     /locus_tag="GCN81_RS03730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nitroreductase"
                     /protein_id="WP_156721994.1"
                     /transl_table=11
                     /translation="MTEDDDVSLRSALTRARSTEEPGPDTSAGPAVTRLGDGGPVVALG
                     AGAGQNQGQGEGLDLDLDLDQILTRSLAAAHADGRLRPAPSAGALHPVNAHVLAGPGCS
                     VPAGRYAYDPVRHRLYGRGHAPAGVADGAVIVLTVTVRRTASHYAHRAWPMALLDAGHA
                     VAALAVAGARRFTLDADAALLAAAAGLPVPGSREERWPDAIPEHPFAAVHFMPPGGVRT
                     SDEADDALLRAWAGDADAVGLRGAGSRRTYPTEPRRAYEVGAAERVLELLGAAPRAGGG
                     TWVRPSAPLPPVDAALRRRSVQPPLPGVPDPDELAAVMAAAAGQRGTRWCLAVGGPRAG
                     LLELASESLELVTLARGDARPTLAVWAARQGWLADSGAVLLAYGCPGGASAVRVRQEHL
                     AAGVGVGLAQVTATALGLRSRPVGSWQGADLGAALGGPTDQDWIIHGLALGRGRTP"
     gene            complement(3983..5362)
                     /locus_tag="GCN81_RS03735"
     CDS             complement(3983..5362)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004118.1"
                     /locus_tag="GCN81_RS03735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_156721995.1"
                     /sec_met_domain="YcaO (E-value: 2.1e-46, bitscore: 153.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALPPEALVDPVCGVVRRVAPVEHPSGAPVRYTAMTADVADARR
                     LGLWPADRVALGTTFGDPEGARIAAIAEAVERYCGNRLPPPGHPDAPVRATAAELASRG
                     LRLYGPGELPRYADWQHARERFPYRPFTEDTPALWARGRERTKDGTSEECWAPVALTHL
                     NWRQGPLRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDP
                     ASVPGLTADLAGCGLDVWIVEMPSEFAPCMAALVRDPVRGLYAAGFACRYDPADAARKA
                     VLEAVHTWVFTQGLTDPDGWVFKAIDAGMLARGLYLDHRGDRRYLDDCGTDFAAVRDLG
                     AHVQVWLDKRVAPLARRFTEPALGVVGVAAVASGSRELLDAALADGGHRVLTFDLTTED
                     VAETPLRVARVLVSDLIPNAPAAFAYLGSPRFGDVALARGWRGEVPVLPGDFTLTPPPH
                     M"
     gene            complement(5359..6963)
                     /locus_tag="GCN81_RS03740"
     CDS             complement(5359..6963)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="GCN81_RS03740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_156721996.1"
                     /transl_table=11
                     /translation="MNTRYLTTPDGTRLATDLQLPDGVGPFPAVLIRTPYDRLRHRAEL
                     RGWAARGFAALAQDVRGRHASHGKWHPYRAEAEDGAAALEWIRRQPWSDGRVVACGASY
                     AAHCALAIAGDAPGTGRTGAARADAVIAAVPALGAAETAREQTGPERLFARAGWWAAHG
                     DRRDSDESALAKALADDPALLAHLPVRDLPRRIGRELPSWAGLWSQRQHYAPPPAPCPP
                     LLAVGGTHDPFAEDTLRLWRGWNGPARLLLGPWGHGLTAEPGSEAGAAHWLRIGELYVD
                     WARAALEGGLDAGRAGAVALGGSELWLSADELAAVPAVHSLDGMRLLRGPAFRADPGLP
                     VRSDDLEVPRTGPADRCLLVGAPLPRPLDLLGPAEARLRVTADTDCADWVVRLVALTVD
                     DVAAPLATGVVRRASGGRGSGVGSALASGSDSGVGSGVASAVGSGVASGVGSEVTVPLG
                     HLARRLPAGTRLRLEIAGHHFPAHARNPHTGADPLTATRLLASERTVDLAASALLLPVA
                     PPRNLTHAVHPVQEICR"
     gene            complement(6960..7826)
                     /locus_tag="GCN81_RS03745"
     CDS             complement(6960..7826)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613004.1"
                     /locus_tag="GCN81_RS03745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156721997.1"
                     /transl_table=11
                     /translation="MTRSHLAPGVSLVAVPDGGCAVRTPDGEFLWVDTGTVAASRLRDR
                     LAGGSGGVDDDVELAALVRAFERAGYLEATQTDRSDTEGASSWPLDRRVILLTGEPALT
                     GPLSALLAECAAEPRTVAPDEVVDAAEAGGAAAVVWCHDEPVPPGLWDAADRLPERGVG
                     WLRCHREGFQVWIEPLAAGPGDVTSAHVRARRLAATPAHRELAAYWEGRRTEQPFPPTP
                     VHRHLVAAHLAADLTAWAQGAPATRRLRLIDLRQARVSEHTVLPVPDVAPLPVAPLPVV
                     AAPLPVS"
     misc_feature    7900..7913
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    7900..7914
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.84"
                     /tool="antismash"
     gene            complement(7933..8046)
                     /gene="amiA"
                     /locus_tag="GCN81_RS32675"
     CDS             complement(7933..8046)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947327.1"
                     /locus_tag="GCN81_RS32675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_267904559.1"
                     /transl_table=11
                     /translation="MDQEKVFTPIAEQGQLAHLSATHSNALVENPFDDGEE"
     misc_feature    8025..8039
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    complement(8026..8037)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(8113..8126)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.1"
                     /tool="antismash"
     misc_feature    8114..8129
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.15"
                     /tool="antismash"
     misc_feature    8114..8132
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            complement(<8115..8913)
                     /locus_tag="GCN81_RS03750"
                     /pseudo=""
     CDS             complement(<8115..8913)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004114.1"
                     /locus_tag="GCN81_RS03750"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTVEGVRFGYPGRSVLDGVDLRVAHGELLALVGLNGCGKSTLLRL
                     AAGLLRPSAGRVLLGGDDLGRLSRRAAARRVALLHQSAPAVPGMTVRQLVRQGRYATRG
                     PLGMLREGDDETSRRALVDAGVADWAARGVDTLSGGERQRVRLAMALAQDTPVLLLDEP
                     TTYLDLRHQLEVLQTVVRLREERALTVVMVLHDLAHAARFAERIVALRGGRVAADGPPG
                     EVVTPALLADVLGVAGRVGRDPEGGWPVCYPDHPLPLAK"
     gene            complement(9092..10087)
                     /locus_tag="GCN81_RS03755"
     CDS             complement(9092..10087)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470990.1"
                     /locus_tag="GCN81_RS03755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron ABC transporter permease"
                     /protein_id="WP_156722346.1"
                     /transl_table=11
                     /translation="MVLVGVLLLVAVVADLVAGRAVSPAGVWQVLLGGGDDTARHIVLQ
                     LRLPRMLVALVAGACLGLAGLVLQSALRNPLAGPEVTGVTPGAVLGAVAATGLGLAGWE
                     SPAAVVVAACVGGFAGAGLLWVLAGRDRGDPEQTAVHGVLVSAVLTGLTAMVLLVAPGE
                     LGSVVQWLVGSIEGRVWDHWNLIWPWALCWTAAACALAGPLTLLRCGDEQAAAVGLATG
                     RARAVALVCAVALTAGAVSVVGALGFVGLLVPHVALALFGADLRIGLPGAALLGATVVC
                     GADAVAQGASRVLTVVLDMDRLTLPTGAVTTFAGAALLLIVVRRVPLSRP"
ORIGIN
        1 aggcgccgtc cagccgcccg cccgccacgg cgtgcgcgac gaccgcgacg accccgagta
       61 cggccgcgac cacgacgacg tcccgcgcgg cggctgccgt cgcctcccac tcctgctcgg
      121 cgcgctgggc cctgcccagt tcctctccct gttcggcgag ttccgtgcgc cgctggtcca
      181 gggcgccgaa ggcgagcagc tcccgtagcc cgtccaccgc ctccacggcg tccgccgaca
      241 gcttcgccgc cgcggcgcgg gtcctggctc cgcgccgggc gcgctcccgt gccccgatca
      301 gcggggcgag ggcgaggagc agcgcggcgg gcaggacggc ggccagcagc cagggttcgg
      361 ctccggcgag aacgactccg cccgccccga acacagctcc gctggcgagg agttgggcgg
      421 tggtgtgggc gtagaagaac tccagcgcct cgacatccgc cagcgcggtc gccgccaggt
      481 cgccgctgcg cagcccgccg atcctggccg gggcgctgcg cgcgagcccg tcgaagaccc
      541 gtacccgcag ctcggccagg acccggtagg ccaggtcatg cgagaggtcc atctcacgcc
      601 aggtcgccac cgcgcgcacc agcaccagca gcgccagcac ggcgacggtg gccgccgacg
      661 gcacgacctg atccaccacc gccgcaccca cggtatgcgc gagcagggtc accagcgcga
      721 caagtgccgc ctgatcgaca aacgccgcga cacaggtccg caccaccatc acccgatgcc
      781 gcgacaaggc gggcaacagc ccccgtagcg cccccgcctc gggctcccgg cccgcctcgg
      841 cctcggtgtc gttgtcgctt ggtccgttca cgcggctcgg tctccttcgt tcgcctcgtg
      901 cccggcgcgt accagccggg cgtacagtcc ctcgtccgag agcaggctgg tgtggtcgcc
      961 gatggcgtcg acgcggccgc cgttcaggac gacgatgcgg tcggcgtgcc gtacggactc
     1021 cagacgatgg gccacgacga tgcaggtgcg tcccgaggcg gctcggagca gttcacggcc
     1081 gatgcgggcc tcgcggcgtt cgtcgaccgc gctggtcgcc tcgtccagga cgaggaccgg
     1141 cgcgtcggcg agcaacgcac gggccagggc gaggcgttga cgctgccctc cggagagggt
     1201 ggcgccgcgt tcgccgagca cggtgccgta gccgtccggg aaggcggcga tctcgtcgtg
     1261 tacgcctgcc gcgcgggccg ccgccgtcag ctcgtcgtcg ctcgcgccgg ggcgggccag
     1321 ccgcaggttc gcggcgatgg tgtcgtggaa gaggtaggtc tcctgggaga cgacggcgat
     1381 gtggcggcgc agcgcgtcga gcccgtacga ggcgaccggc tccccgtcga ccgtcacctc
     1441 accccgctgc gactcccgcc cgcgcagcag cagggcgagc agggtggact tcccggcgcc
     1501 cgacgggccg acgatcgcgg tcgtacggcc gccttcggcg gtgaagctga cgccgtccag
     1561 ggcgggccgc gcggcgtccg gataggtgaa ccgcacgtcg tcgaagcgga gttcgggcgg
     1621 tgccttccag tcggccggac gccgcccgct gtccgcgatg tcgggccgcg cggtgcgcag
     1681 ggccgcgatg ccgtcggccg ccgagacacc gaggtatccg gcgtgccact cgcgcgacag
     1741 gtcacggatc gggcggaagc actcggaggc gagcatcagc agcaggtacg tactgacggg
     1801 cgccgcgtgc ccgcccccgg ccgcgaccgc cgccagcagc gccgccgcca cggtgccgcc
     1861 ctgtacggcg aggtcggtca gtccggtgtc gaccagcgac acccgcagct tggcgaccgt
     1921 ggcacggtgc agcgccgccg agcgccgctc cagcgactcc ctggtacggc ccaccgcgcc
     1981 cgccgcgcgc agcgatggca tgccttgcag cgcctccagg tagtcggcgg cgagcccttc
     2041 gtaggagtcc cagtgctcct tgcctcggcg ggccagcagc cggtcccacc agcgcggcgc
     2101 cagcagcgcc agcgccaacg cggggaccag cgcgaggagt acgtacggct ggaggacgcc
     2161 gaggggtaca agcagcagcg gcggcaccag gcaggtgatg gccaactgcg ggaggtagcg
     2221 ggagagatac gcgtcgacgc cctccacgcc gtccaccagc gtggcccgta cggcgcccgc
     2281 cctggcggtc gtcagatgcg cgggccccag ctgtccgagc cgctccagca gagcgtcccg
     2341 cagccgtacc cgggccagcg ccccggcccc caccgccact ctgcgctggc ggcgcccgag
     2401 ccagaaccgc gtcgcgacca cggccagcac cgcgccgagc agcgcgggca gttcaccggt
     2461 ggcgccccgg gcgagtgcgg ccagggcgag ggccagcagt acggcctgcg cgaggtgggt
     2521 cgcggcgacg gcgccgagca gcgcggtggc ggcggtgagc gggccgcgta cggtgcgggc
     2581 cgcggtgagc agttcccggt gcagcatcat ggggtgcggc cccttccgag ggcgagtccg
     2641 tggatgatcc agtcctggtc ggtgggtccg ccgagcgcgg cgccgaggtc ggcgccctgc
     2701 caggacccga cggggcgcga gcgcagaccc agcgcggtcg cggtgacctg ggcgagcccg
     2761 acgccgaccc cggccgcgag gtgctcttgg cgcacccgca cggcggaggc gccgccgggg
     2821 cagccatacg ccagcagcac ggctccggag tcggccagcc agccctggcg ggccgcccac
     2881 acggcgaggg tgggacgggc gtcgccgcga gccagtgtca ccaactccag gctttcgctg
     2941 gcgagttcga gtagtccggc gcgaggcccg ccgacggcca gacaccaccg cgtgccgcgc
     3001 tgcccggccg ctgccgccat cacggctgcc agctcgtcgg ggtcgggcac gccgggcagc
     3061 ggcggctgga cgctacggcg acggagtgcg gcgtcgaccg gtggcaacgg ggcggaaggc
     3121 cggacccagg taccgccgcc tgcccggggc gctgctccga gcagctccag gacccgctcg
     3181 gccgcaccca cctcgtacgc acggcgcggc tcggtcggat acgtacggcg cgaaccggca
     3241 ccccgcaagc cgacggcgtc agcatcgccc gcccaggcgc gcagcagcgc gtcatcggcc
     3301 tcatcggacg tacgcacccc gccgggcggc atgaaatgca cggctgcgaa agggtgctcg
     3361 gggatcgcgt ccggccatcg ctcctcccgc gaacccggca cgggcagccc cgccgcggcg
     3421 gcgagcagcg ccgcgtcggc gtcgagggtg aaccgccgcg ccccggcgac ggccagcgcc
     3481 gccaccgcgt gaccggcgtc cagcagcgcc atcggccagg cacgatgggc gtaatgggac
     3541 gcggtgcgcc gcacggtgac ggtcagcacg atcaccgcgc cgtcggccac accggcaggc
     3601 gcgtgcccac gtccgtagag ccggtgccgg acggggtcgt acgcgtatcg ccctgccggt
     3661 acggagcatc cgggcccggc cagcacatgc gcgttcaccg ggtgcagggc gccagcggag
     3721 ggcgccgggc ggaggcgccc gtcagcgtgc gcggcggcca acgaccgggt cagaatctga
     3781 tccagatcca gatccagatc aagcccctcg ccctgcccct ggttctggcc cgcccccgca
     3841 cccagcgcca ccaccggacc gccgtcgccc agacgcgtca cggcaggccc cgcggacgtg
     3901 tccgggcccg gctcttcggt ggaccgcgcg cgggtgagcg ccgaccgcag cgacacgtcg
     3961 tcgtcctcgg tcatcgcgcc gctcacatgt gcggtggcgg cgtgagcgtg aagtcccctg
     4021 gcaagaccgg gacttcacca cgccacccgc gtgccagcgc cacgtccccg aaccgtgggc
     4081 tgcccaggta ggcgaaagcg gcgggcgcgt tcggtatcag atcggagacc agaacgcggg
     4141 caacccgcag cggggtctcg gcgacgtcct cggtcgtcag gtcgaaggtc agcacacggt
     4201 ggccgccgtc cgcgagagcc gcgtccagca gctcacggct gccggaagcg accgcggcga
     4261 cgccgaccac acccagcgcc ggttcggtga aacgccgggc gagcggggcg acccgcttgt
     4321 ccagccacac ctggacatgg gcgcccaggt cacgcacggc cgcgaagtcc gtgccgcagt
     4381 cgtccagata gcgccggtcg ccccggtggt cgaggtagag gccccgggcc agcatgcccg
     4441 cgtcgatcgc cttgaagacc cagccgtcgg ggtcggtgag cccctgggtg aacacccagg
     4501 tgtgcacggc ttcgagtacc gccttgcggg ccgcgtcggc cgggtcgtac cggcaggcga
     4561 accccgccgc gtacagcccc cgtaccggat cgcgcaccag cgcggccatg cacggcgcga
     4621 actcggacgg catctcgacg atccacacat ccagtccgca cccggcgagg tcggccgtca
     4681 gtccgggcac gctggccggg tcgataccgc gggtcggccc gtccaggtgc caccacagct
     4741 ccagcgcgtc ccgttcgacg acttcgagga gtccgcgctc cacggcgtcg tccaggccct
     4801 ggccggtggc gatccccgcg tagttgaggt ggtgtgtgcg cgggagtgaa cgcaatggcc
     4861 cctggcgcca gttgaggtgg gtgagggcga cgggcgccca gcactcctcg ctcgtgccgt
     4921 ccttggtgcg ttcacggccg cgcgcccaca gggcgggggt gtcctcggtg aacggccggt
     4981 acgggaagcg ttccctggcg tgctgccagt cggcatagcg ggggagctcg ccgggtccgt
     5041 acaaccgcag cccgcgtgag gcgagttcgg cggccgtggc gcgcaccggc gcgtccggat
     5101 gtccgggggg cggcagccgg ttgccgcagt agcgttcgac ggcctcggct atcgcggcga
     5161 tccgcgctcc ttcggggtcg ccgaaggtgg tgccgagggc gacgcggtcg gcgggccaca
     5221 gcccgagccg ccgggcatcg gccacatcgg cggtcatcgc ggtgtagcgg accggtgcac
     5281 cgctggggtg ctcgacgggg gcgacacggc ggacgacacc gcagacgggg tccaccagcg
     5341 cctctggcgg cagcgcggtc atcggcatat ctcctgtacg gggtgcacgg cgtgggtgag
     5401 gttgcggggt ggtgcgacgg ggagcagcag cgcgctggcg gcgaggtcga cggtgcgctc
     5461 ggacgcgagc agccgggtgg cggtcagcgg gtcggcgccg gtgtgcgggt tgcgggcgtg
     5521 ggcggggaag tggtgtcccg cgatctccag gcgcagccgg gtgcccgcgg gaagccgccg
     5581 ggcgaggtgc ccgagcggaa cggtcacctc cgagccgacg cccgaggcga cgcccgagcc
     5641 gacggccgag gcaacgcccg agccaacgcc cgagtcgctg cccgaggcaa gggccgagcc
     5701 gacgcccgag ccgcgtccgc cgctcgctcg gcgcacgacg ccggtggcca gcggcgcggc
     5761 cacatcatcg acggtgagcg ccaccagccg tacgacccag tcggcgcagt cggtgtcggc
     5821 cgtcacccgc agccgtgcct cggccgggcc gagcaggtcg agcgggcgcg gcagcggtgc
     5881 gccgaccagc aggcagcggt cggccgggcc ggtccgggga acctccagat cgtcggagcg
     5941 caccgggagc cccgggtccg cgcggaaggc ggggccgcgc agtagccgca tcccgtccag
     6001 cgaatgcacg gccgggacag cggccaactc atcggcggaa agccatagtt cgctcccgcc
     6061 gagggcgacg gctcccgcgc gcccagcgtc gagcccaccc tccagcgccg cgcgcgccca
     6121 gtccacgtac agctcaccga tgcgcagcca gtgcgccgcg cccgcctcgc tgccgggctc
     6181 cgcggtgagg ccgtggcccc agggtccgag cagcagccgc gccgggccgt tccagccgcg
     6241 ccacaggcgc agggtgtcct cggcgaaggg gtcgtgggtg ccgccgaccg cgagcagggg
     6301 cgggcagggg gcgggcggcg gggcgtagtg ctgccgctgg ctccacagcc ctgcccagga
     6361 gggcagttcg cggccgatgc ggcgcggcag gtcgcgtacg gggaggtggg cgaggagcgc
     6421 ggggtcatcg gccagcgcct tggcgagggc gctctcgtcg gagtcacgcc ggtcaccgtg
     6481 cgcggcccac catccggccc gcgcgaacag ccgctcaggg cccgtctgtt cgcgcgcggt
     6541 ctcggcggcg cccagcgcgg gtacggcggc gatgacggca tcggcgcgcg cggcgcccgt
     6601 acggccagta cccggggcgt caccagcgat cgcgagggcg cagtgcgcgg cataggaggc
     6661 gccgcacgcc acgacccgcc cgtcggacca gggctgtcgg cgaatccact ccagcgcggc
     6721 ggccccgtcc tcggcctcgg cccggtaggg gtgccacttc ccgtgtgagg cgtgccgccc
     6781 ccgtacgtcc tgcgcgagtg cggcgaaccc gcgggcggcc cagccgcgca actcggcgcg
     6841 atggcgcagc cggtcgtagg gcgtgcggat caggacggcg gggaaggggc cgacgccgtc
     6901 cgggagttgg aggtcggtgg cgagtcgcgt tccgtccggg gtggtcaggt agcgggtgtt
     6961 catgagacgg gcaggggcgc ggccacgacg ggcaggggcg cgacgggcag gggggcgacg
     7021 tcaggaaccg gcagcaccgt gtgctcactg acgcgcgcct gacgcaggtc gatcaggcgc
     7081 agccgacggg tggccggggc gccctgcgcc cacgcggtga gatcggccgc gaggtgggcg
     7141 gcgaccaggt ggcggtgtac gggagtcggc gggaacggct gctcggtgcg acggccctcc
     7201 cagtaggcgg cgagttcgcg gtgcgccggg gtcgccgcca ggcggcgcgc ccggacatgg
     7261 gcggacgtca cgtcgccggg accggcggcg aggggttcga tccacacctg gaagccctcg
     7321 cggtggcagc gcagccagcc gacgcctcgc tcgggcagcc ggtccgcggc gtcccacaga
     7381 ccgggtggta cgggctcgtc gtggcaccag acgacggccg cggctccgcc ggcctccgcc
     7441 gcgtcgacga cctcgtccgg ggcgacggtg cgcggctcgg ccgcgcactc ggcgagcagg
     7501 gcggacagtg gtccggtgag ggcgggttcg ccggtcagca ggatgacgcg ccggtcgagc
     7561 ggccaggagg acgccccttc ggtgtcactc cggtccgttt gtgtggcctc gaggtagccg
     7621 gcgcgctcga acgcccggac cagggcggcg agttcgacat cgtcgtccac gccgccgctc
     7681 ccacccgcga gccggtcccg gagccgcgag gcggcgacgg taccggtgtc gacccacaga
     7741 aactcaccat cgggcgtacg gaccgcgcac ccgccgtccg gcacggcgac gaggctcact
     7801 ccgggcgcga ggtgactgcg cgtcatgtct ggtgtctcca aggggatagc gggtcgggcg
     7861 gtcgggggtc cggcggtcga gaacgggacg ggcccgcccg gaagaagcgt tccgggcggg
     7921 ccctggagtg gcttactcct cgccgtcgtc gaaggggttc tcgacgagcg cgttggagtg
     7981 ggtcgcggac aggtgcgcca gctggccctg ctcggcgatc ggggtgaaca ccttttcctg
     8041 gtccatgagt tgagcccttc cttgagattg cgatgggtgt ggcggctccg gccggggcca
     8101 cacgagagac agtaatgaaa atgattgtca ttttgcaagt ggtagcgggt gatctgggta
     8161 acacaccggc cagccaccct ccggatcacg tccgacccgc cccgccacgc ccagcacgtc
     8221 agccagcaga gcgggcgtca ccacctcgcc gggcggcccg tccgcggcca cccgcccgcc
     8281 acgcaacgcg acgatccgct ccgcgaaacg agccgcgtgc gcgaggtcgt gcagcaccat
     8341 caccacggtg agcgcccgct cctcgcgtag cctcaccacg gtctgcagga cctccagttg
     8401 gtgccgcaga tccagatacg tggtcggctc gtccaggagc agcacgggag tgtcctgggc
     8461 cagtgccatc gccagccgta cccgctggcg ttcgcccccg gacagcgtgt cgacaccccg
     8521 cgccgcccag tcggccacgc ccgcgtcgac gagcgcgcgg cggctggtct cgtcgtcgcc
     8581 ttcgcgcagc atgccgaggg ggccacgggt cgcgtagcgg ccctgccgta ccagttgacg
     8641 tacggtcatg cccggtaccg ctggcgcgga ctggtgcagc agggcgacgc gccgggccgc
     8701 cgcgcgccgc gagaggcgac ccaggtcgtc tccgccgagc aggacgcgcc ccgcgctggg
     8761 gcgcagcagc ccggcggcga gccgcagcag ggtgctcttg ccgcacccgt tcagcccgac
     8821 cagggccagc aactcgccat gggcgacccg tagatccacg ccgtcgagga cggaacgccc
     8881 cgggtaaccg aagcgaaccc cttcgacggt gatggcatca ccggtagccg ttgccgaagc
     8941 ggtagccgtt gccgaagcgg tagccgttgc cgcagaggga ggcggtggcg ggggcacggt
     9001 ggcctcagcc tggtcaagct gctggctctc ccccgcgcgg ggtactgcgg aatgcgtcat
     9061 gtcagtcctc ttcgattggt caggggcgcc ctcagggacg cgacaggggc acccggcgca
     9121 ccacgatcag gagcagcgcc gccccggcga acgtggtcac cgccccggtc ggcagagtca
     9181 gccggtccat gtccagcacc accgtgagca cccgcgacgc gccctgcgcc acggcatcgg
     9241 cgccgcacac cacggtcgcg ccgagcagtg cggcccccgg cagcccgatc cgcagatccg
     9301 cgccgaacag cgcgagcgcg acgtgcggca ccagcagccc gacgaaaccg agcgcgccga
     9361 caacggacac cgcgcccgcg gtgagggcga ccgcgcacac cagtgccacc gcacgggccc
     9421 tgccggtcgc gaggcccacg gccgcggcct gttcgtcgcc gcagcgcagc agggtgagcg
     9481 gtccggcgag cgcgcaggcg gccgccgtcc agcacagcgc ccagggccag atgaggttcc
     9541 agtggtccca gaccctgccc tcgatggaac cgaccaacca ctggaccaca ctgcccagtt
     9601 cgccgggagc caccaggagc accatcgcgg tcagcccggt gagcaccgcc gagaccagta
     9661 cgccgtgcac ggccgtctgt tccgggtcgc cccggtcgcg cccggccagt acccacaaca
     9721 acccggctcc ggcgaacccg cccacacagg ccgccacgac gacagccgcg ggcgactccc
     9781 atccggcgag cccgagcccg gtggcggcca cggccccgag tacggcaccc ggggtaacgc
     9841 cggtgacctc gggcccggcc agcggattgc gcagagccga ctgcagtacg agaccggcga
     9901 gcccgagaca ggccccggcg accagcgcca ccagcatccg gggcagccgc agttgcagca
     9961 cgatatgccg cgccgtgtcg tcaccgccgc cgagcagcac ctgccacact ccggcgggcg
    10021 acacggcacg ccccgcgacc agatcagcca cgacggcgac cagcagcagc acaccgacca
    10081 gcaccatccg acgcgccacg gccggaccgc cgccgttgtc cccagcgccg ccgtccacgc
    10141 tcaccgcgct caccgcgctc tccggccccg cctcacaccc agcagcagcc ctcacgtcac
    10201 tcccccgtcc accagctcct ccgtacgcac ccccgcgcga cccgcgtccg acgacggctt
    10261 cttccgtttc ttccgtttct tccgtacgcg gtcacgggtc cgcagcaacg ccacccccac
    10321 gggcaccccg aggagcgccg tccacgcccc gaccggtatc tc
//
