LOCUS       NZ_WOFH01000008        10933 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold8, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000008
VERSION     NZ_WOFH01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 449359)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 334780
            Orig. end                         :: 345713
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10933
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5933](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5933
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(778..1437)
                     /locus_tag="GNZ18_RS23660"
                     /old_locus_tag="GNZ18_23660"
     CDS             complement(778..1437)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619040.1"
                     /locus_tag="GNZ18_RS23660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23660"
                     /product="DUF4360 domain-containing protein"
                     /protein_id="WP_214619930.1"
                     /transl_table=11
                     /translation="MRKAMTASAACAAALALAGGMAPSASATTDDPPPPDRIVIDVLTV
                     NGSGCPAGTTAVATASDNTAFTVTYSNYLAQAGGDSRATDFRKNCQINLRVHVPQGFTY
                     AIAGADYRGFAHLEDGASGLERANYYHQGMQQSTPVSHTIKGSFSDDWQFSDRTPVTEL
                     VFKPCGEDRNFNVNTELRVNKGSSDAAKTSFMAFDSTDGSVKTTYHFAWKRCNAPR"
     gene            complement(1540..2193)
                     /locus_tag="GNZ18_RS23665"
                     /old_locus_tag="GNZ18_23665"
     CDS             complement(1540..2193)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619040.1"
                     /locus_tag="GNZ18_RS23665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23665"
                     /product="DUF4360 domain-containing protein"
                     /protein_id="WP_156218711.1"
                     /transl_table=11
                     /translation="MRKGIAISASALAMTATAAVAAPPATASAPYLTRGPSGVTIELAT
                     VNGSGCPLGTAAVAVSDDKEAFTVTYSNYLVQVGGNSNSTDFRKNCQLNMKVHVPQGFT
                     YAILSADYRGFAQLEPGASGLEKASYYFQGMPTTGRAEHTLKGEYSDNWTFTDVNDVAQ
                     LVYKPCGEERNFNINTELRVNKGTSDPTKVSMMAMDSTDTSIKTTYHFAWKKCP"
     misc_feature    complement(2153..2167)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     misc_feature    complement(2303..2316)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.61"
                     /tool="antismash"
     gene            2688..3560
                     /locus_tag="GNZ18_RS23670"
                     /old_locus_tag="GNZ18_23670"
     CDS             2688..3560
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634760.1"
                     /locus_tag="GNZ18_RS23670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23670"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_156218712.1"
                     /transl_table=11
                     /translation="MGSLIGRNLLAGSEPGVAADAGYADAPERVGFFTDTSVCIGCKAC
                     EVACKEWNAVPEDGLEFTGMSYDNTQGLGSDTWRHVAFIEQDRPVGTDAAGGGLRWLMS
                     SDVCKHCTHAACLDVCPTGSLFRTEFGTVVVQQDICNGCGYCVPACPYGVIDQRKEDGR
                     VWKCTLCYDRLGAGQEPACAKACPTDSIQFGPLEELRERAAARVDQLHDLGVDEARLYG
                     ADPEDGVGGDGAFFLLLDEPEVYGLPPDPVVTTRDLPSMWRHAGAAALTLLGGAVAVFA
                     AHGRRGGRR"
     gene            3557..4528
                     /gene="nrfD"
                     /locus_tag="GNZ18_RS23675"
                     /old_locus_tag="GNZ18_23675"
     CDS             3557..4528
                     /codon_start=1
                     /gene="nrfD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656501.1"
                     /locus_tag="GNZ18_RS23675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23675"
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="WP_156218713.1"
                     /transl_table=11
                     /translation="MSPPEREERARGRRRRRGEQAMVPEAEFSSYYGRPVLKPPRWKAP
                     DIAGYFFLGGLAGAGSVLAAGSQLTGRPAAARALKISSLAAIGGSTAALIHDLGRPARF
                     HHMLRVLKPTSPMSLGSWLLMAYGPAAGGAAVLDVARLFPRLGAAATAGAAVLGPAVAS
                     YTAVLAADTAVPAWHGAYRELPFVFAGSAAAAASGMALLTAPPAETGPARTAAVAGAVL
                     EGAASRLMRRRLGMVAEVYEGGRAGGLTRAAEILAVAGAAGAALLAGRNRAAAALSGAA
                     LLAASACTRFGVFYAGVRSAEDPKYTVVPQRERLDARAGAAS"
     misc_feature    complement(4647..4662)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.53"
                     /tool="antismash"
     misc_feature    4675..4690
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            4741..5004
                     /locus_tag="GNZ18_RS43270"
                     /old_locus_tag="GNZ18_23680"
     CDS             4741..5004
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS43270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_23680"
                     /product="hypothetical protein"
                     /protein_id="WP_156218714.1"
                     /transl_table=11
                     /translation="MNGAIHGGGELHVCMTRNACAGPGGCGYLRVEAERSVPGGAAWRR
                     ARRVFEQRMFEADAPPGPGLAPPGGPVPGRGGAGAGGTTSGS"
     gene            5001..5933
                     /locus_tag="GNZ18_RS23685"
                     /old_locus_tag="GNZ18_23685"
     CDS             5001..5933
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="GNZ18_RS23685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23685"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_156218715.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-96, bitscore: 316.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAESAERAPAPRARAGASPRDRPGAPPGGDTSGLGVGLRSSHLA
                     QILAEWPDVGWFEIISENFMDSGGYPRHALGRIAERYPVVMHGVSMSIGSTDPLDFGYL
                     ARLRRLADSTGARWVSDHVCWTGVAGVNTHELLPLPFTERSLRHLVRRVGVVQDVLERR
                     LVLENPSAYAAFAHSTMPEWEFLARLCEESGCGLLLDVNNVHVSAFNNGFDPEEYIRAI
                     PHDRVVQIHVAGHADMGDHLVDTHDGPVAEPVWDLYRLATSLTGPVPTLLEWDDRLPPL
                     PDLLAELHKARDHLGAPGPGLRDAGASDG"
     misc_feature    5850..5852
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5926..6735
                     /locus_tag="GNZ18_RS23690"
                     /old_locus_tag="GNZ18_23690"
     CDS             5926..6735
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="GNZ18_RS23690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23690"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_156218716.1"
                     /transl_table=11
                     /translation="MGSATPSLDAAQQWLLHAVTTPSASAEQAGAMLAGTDGLDAARRL
                     EIYRVGYRRRLLGTMRHMLPALRELLGPDLFDVFAAGYLDAHPPRAYTLARLGDRFAAY
                     LEEQRPDRDLPAGEREPWADLMVDLVRYERLFAEVYDGPGTEEEPPAPWPTGLPDRVRG
                     APGVRLLRACAPVHEYHSAVRRGARPAPPAFVPSRIVVFRRNYRVVTASPPADAFTLLE
                     ALMGGAPPEQASVRAGLAPADARRHLRRWLAQRWITALPASPARESP"
     gene            complement(6745..7506)
                     /locus_tag="GNZ18_RS23695"
                     /old_locus_tag="GNZ18_23695"
     CDS             complement(6745..7506)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS23695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_23695"
                     /product="hypothetical protein"
                     /protein_id="WP_156218717.1"
                     /transl_table=11
                     /translation="MIDDQTLMSSRHVLCVLGTGLDLDAVEAAAAREGFELDWEYSGTG
                     ADPRMPEAFEACVARASFTRADWDAVEAHDTVAYVLSPPVLPGVAFTVARRTLVLTADL
                     LRSGATAVKNESSGLTHGRDRWLDLAAGVYAGGAEESAVPLYRAWVKRPIADGPLLHSC
                     GMHLLGAPDIELAPPEDPAASPSPDEAAALIDGLALYLLTERRAEEIEDGEGFRLAPDA
                     PRWILRHHPCDRYEADDFFFNPHGYWRLAAE"
     misc_feature    7580..7594
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            7685..8338
                     /locus_tag="GNZ18_RS23700"
                     /old_locus_tag="GNZ18_23700"
     CDS             7685..8338
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS23700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_23700"
                     /product="hypothetical protein"
                     /protein_id="WP_156218718.1"
                     /transl_table=11
                     /translation="MSTGVDDSARRRFDLTMLHAAYGAFRRDMAAMAVAGRVASWGPFT
                     AEMGPFPVDANAWSLFRFHWLAQQAAERRALWTVIRGRVTGGRSAALDAMDVEAVRLVA
                     LMDRVDAALEHEDDDILLPLTRDLPAAVEAYLDFKEANVLPLIHENLTPYEWGTYDVEF
                     RQEIGVRGLRVFLPWLLDGASEATCGAVLRLLPPPVRLAYRTRWLPRYRRALRR"
     misc_feature    complement(8403..8418)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.67"
                     /tool="antismash"
     gene            8489..10165
                     /locus_tag="GNZ18_RS23705"
                     /old_locus_tag="GNZ18_23705"
     CDS             8489..10165
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131545.1"
                     /locus_tag="GNZ18_RS23705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23705"
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="WP_156218719.1"
                     /transl_table=11
                     /translation="MAGNRAPGGRAADGRAGGRGSGGAVGSVSRALDERFGTAGFARKA
                     LRKAFPDQWSFLVGEIAMYSFVIILLTGVYLTLFFKPSMHEVIYNGSYTKLRGVKMSEA
                     YASSLKISFDVRGGLLVRQIHHWATLIFMGSILVHLLRNFFSGAFRKPRELNWIIGVTM
                     LVLVMVNGLFGYSLPDDLLSGTGIRILEGVIAGIPLVGSYLVMFVFGGEFPGSDIIPRL
                     YVVHVLLIPGLLAALIPLHAVVLTWRQTHTQFPGKGSTNTTVRGYPFFPVFMAKTSALF
                     LWVLGVTTLLAAFVQINPIWLFGPYDPGAISAGSQPDWYMGWLEGSLRIMPAWEITAWG
                     HTVSMSVLVPALVVPGVLFTGLAVYPFVERWVTGDHQIHHLLDRPRDVPARTGIGVGGI
                     VFYGLLWLAGGNDVLANKFDVSLYATTWFFRVAVILGPVLGYIVAYRICLGLQRRDLGL
                     VEHGLETGIIRQSPDGRFSEVERHLPEERIAAITDPRPAPAVDLTLPPPVDGVDDPRGR
                     HLKGRVRAALNRRFRADLATAPERPEPSEQRELAESEHGPR"
     gene            complement(10198..10809)
                     /locus_tag="GNZ18_RS23710"
                     /old_locus_tag="GNZ18_23710"
     CDS             complement(10198..10809)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634391.1"
                     /locus_tag="GNZ18_RS23710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_23710"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_156218720.1"
                     /transl_table=11
                     /translation="MHAAVGAIAESGPATWSLRELARRAGVSHAAPAHHFGDKTGLLTA
                     VAAEGYTLFADALERAGEDLYDVGLAYVRFAVDHRAHFEVMFRPELYRADDPEVNAARR
                     RADEILRHGVRTVAPAGAPRETHRPDETDETGLDGETGLDGETGLDGETGLDGDRTAAL
                     AAWCIVHGFAELWLSGAFSGDLGEDPVAAASPVIRRLFAR"
ORIGIN
        1 caggccggtc acggtgaaca cgcggcggac gcgctcctgc agccccgcgg cgaccaggct
       61 gacgccgcgg ccgtgcgcgc ggttgtaggc gcgggcgaag gcgttcaggc cgctggagtc
      121 gcagaactcc acgccggcca tgtccacgat caccgccagg ccggtcgcgg tgctcgcctg
      181 ttccaggcac tcggccagca gcgtgtgggt gtggatgtcg accgtgccgg tggcggtgac
      241 cagggcgtag ccgcccaggg ccgaccggtg cagggtcagg gtggacccgc cgttcaccgg
      301 cggccgcccg acgtgtggcc ttgggcggtg gagcgcatct ggccgggccg gtcgaagtac
      361 cagcctgggc cggggtcgtt cgagggagcc acacgatcac ctccggggac atgacccact
      421 cggccgaata gtatttcagg taaagagctg catgtgtagc agggacggcg ccgatttcgc
      481 cgtcggtccc aacgcctcag gcggccctcc ccgcggcttg cgtccggcgc ggctcgcggt
      541 cgagatcggt accgtctggt ccccggccac ggccttctcg gccgagtcgc ggaggagcgg
      601 attgttcgtg ctggtcaggt gccatcggac gagccgccca cgaaggcgcg gccacctcgc
      661 gcggtggcac ccgcatgcgg aaggccccgc cggcgggcat gagagtaggg ccgcgccctt
      721 ccccgagtaa ggacgcggcc ctgttcctct atggccgtga gcggggcccg cctcgcgtta
      781 gcgaggcgcg ttgcagcgct tccaggcgaa gtggtacgtc gtcttcacgc tgccgtcggt
      841 ggagtcgaag gccatgaagc tcgtcttggc cgcgtccgag ctgcccttgt tcacgcgcag
      901 ctcggtgttg acgttgaagt tgcggtcttc accgcagggc ttgaagacca gttcggtgac
      961 cggcgtgcgg tcggagaact gccagtcgtc cgagaacgag cccttgatcg tgtggctcac
     1021 cggggtcgac tgctgcatgc cctggtggta gtagttcgcc cgctccagac cggacgcgcc
     1081 gtcctccagg tgcgcgaagc cgcggtagtc ggcgcccgcg atcgcgtagg tgaagccctg
     1141 cggcacgtgc acccgcaggt tgatctggca gttcttcctg aagtccgtcg cccgcgagtc
     1201 gccgccggcc tgggccaggt agttgctgta ggtgacggtg aacgcggtgt tgtcgctcgc
     1261 cgtggcgacc gcggtggtgc ccgcggggca gccggagccg ttcacggtga ggacgtcgat
     1321 gacgatcctg tccgggggcg gcgggtcgtc ggtggtggcg gacgcggacg gtgccatacc
     1381 gccggccagg gccagtgcgg cggcgcacgc cgccgaagcg gtcattgcct tgcgcatggg
     1441 tgttcgtctc ctaactcggg tggagagtgt ttctcgggga cacgcagctg ccgttctaga
     1501 cgcaattccc ggcagttgcg atgtgaaggg aaggctggac taggggcact tcttccaagc
     1561 gaagtggtag gtcgtcttga tgctggtgtc ggtcgagtcc atggccatca tgctgacctt
     1621 ggtcgggtcc gatgttccct tgttgacgcg cagctcggtg ttgatgttga agttgcgctc
     1681 ctcgccacac ggcttgtaca cgagctgcgc gacgtcgttc acgtccgtga acgtccagtt
     1741 gtcggagtac tcgcccttca gggtgtgctc cgcccgaccg gtggtgggca tgccctggaa
     1801 gtagtagctg gccttctcca gaccgctggc accgggctcc aactgggcga agccgcggta
     1861 gtcggccgac aggatcgcgt aggtgaagcc ctgcgggacg tgcaccttca tgttgagctg
     1921 gcagttcttg cggaagtcgg tcgagttcga gttcccgccc acctgcacca ggtagttgct
     1981 gtaggtgacg gtgaacgcct ccttgtcgtc ggacacggcg acggccgcgg ttccgagggg
     2041 gcagcccgag ccgttcaccg tcgccagttc gatggtgacg ccgctcggac cgcgggtgag
     2101 gtagggggcg gaggccgtcg ccggcggggc ggcgacggca gccgtggcgg tcatcgccag
     2161 ggctgaggcg gaaatagcga tccctttacg catgcgattt cctcttcgct gggggcggac
     2221 gcggttttct acggccgaag agttggaacg tgatccagga tctgcccagg agagtagacg
     2281 gcgccgatca agccggtcaa cgttctcttt ttaacttccc ttgaatgtcc gggccgcccc
     2341 acatgcaatc tcattccgta accgagcgaa aacccgtcca atctgcgaaa acggacggtc
     2401 caacaaacaa gagggtgggg acattggccg gggaccgttc cagaaacagg gtcaatcgaa
     2461 tttcaggaac cgttcagagt cgctagaaga gtggccccgc ctggcgcggg acgcgggggc
     2521 gccggccgcc cgcggggacg ccgggcggcc cggcggggca ccggggccgc gcacccgaaa
     2581 tcgccgccca ccggcggtcg aacgcctgga aagcggcacc ggccgaggtg tagggggcac
     2641 gacgcgggga agggtgcctg cgcctggccg tcgtgggagg tcgtgggatg ggcagcctga
     2701 tcggacgcaa cctgctcgcc ggatccgagc ccggcgtcgc cgccgacgcc gggtacgccg
     2761 acgcgccgga gcgggtgggc ttcttcaccg acacctcggt gtgcatcggc tgcaaggcgt
     2821 gcgaggtcgc ctgcaaggag tggaacgccg tccccgagga cgggctggag ttcacgggga
     2881 tgtcctacga caacacccag gggctgggct ccgacacctg gcggcacgtc gcgttcatcg
     2941 agcaggacag gccggtcggg acggacgccg ccgggggcgg cctgcgctgg ctgatgtcct
     3001 ccgacgtctg caagcactgc acgcacgccg cctgcctgga cgtctgcccc accggatcgc
     3061 tgttccgcac ggagttcggc accgtcgtcg tccagcagga catctgcaac gggtgcggct
     3121 actgcgtgcc cgcctgcccc tacggggtga tcgaccagcg caaggaggac ggccgcgtct
     3181 ggaagtgcac gctgtgctac gaccggctcg gggccgggca ggagcccgcg tgcgcgaagg
     3241 cgtgccccac ggactcgatc cagttcggtc ccctggagga gttgcgggag cgggccgccg
     3301 cgcgcgtgga ccagctccac gacctggggg tggacgaagc gcgcctctac ggcgcggacc
     3361 cggaggacgg cgtcggcggc gacggcgcgt tcttcctcct gctggacgag cccgaggtgt
     3421 acgggctgcc cccggacccc gtcgtcacga cccgcgacct gccctcgatg tggcggcacg
     3481 cgggcgccgc cgcgctgacg ctgctcggcg gggccgtcgc ggtcttcgcc gcccacgggc
     3541 gccggggagg acgccgatga gcccccccga gcgtgaggag agggcgcgcg gacgccggag
     3601 gcggcgcggc gagcaggcga tggtgccgga ggcggagttc agctcctact acggcagacc
     3661 cgtcctcaag ccgcccaggt ggaaggcgcc ggacatcgcc gggtacttct tcctcggcgg
     3721 cctggcgggc gcgggctcgg tgctcgcggc gggctcgcag ctcaccgggc gccccgccgc
     3781 cgcgcgcgcg ttgaagatct cctcgctggc ggcgatcggc ggatcgaccg ccgcgctgat
     3841 ccacgacctc ggacggcccg cccggttcca ccacatgctg cgcgtgctca agccgacctc
     3901 gccgatgagc ctcggctcgt ggctgctgat ggcctacggc ccggcggcgg gcggcgccgc
     3961 cgtcctggac gtggcgcgcc tgttcccccg gctcggcgcg gccgccacgg ccggcgcggc
     4021 cgtgctcggg cccgccgtcg cctcctacac cgccgtgctc gcggcggaca cggccgtccc
     4081 cgcctggcac ggggcgtacc gggagctgcc gttcgtgttc gcgggctcgg ccgcggccgc
     4141 cgcgtccggg atggcgctgc tcaccgcgcc gcccgcggag accgggcccg cgcgcaccgc
     4201 ggcggtggcg ggcgcggtgc tggagggcgc cgcgtcccgg ctgatgcggc ggcggctcgg
     4261 catggtggcc gaggtgtacg aggggggccg ggcgggcggc ctgacgcgcg cggccgagat
     4321 cctggccgtc gcgggcgccg ccggggccgc gctgctcgcc gggcggaacc gcgcggcggc
     4381 cgccctcagc ggggcggcgc tgctcgccgc gtccgcctgc acgcgcttcg gggtcttcta
     4441 cgcgggcgtg cgctcggcgg aggacccgaa gtacacggtg gtcccccagc gcgaacgcct
     4501 ggacgcccgt gcgggggccg cctcctagcg cgactccccg tcataacggc gcacccgtat
     4561 ctcccgtcgg ccctgcccgg aggacaacgg ccgaaccggt cttttaccag gccatcgccg
     4621 tcgcggcaca ctccgctctg ggagaggtga cataaatctg acctgccatt gactactgag
     4681 tgtgattagc ctgttgcgag acgtcatccc ggaaatgatc tcgacgatgg gaggcacccg
     4741 gtgaacggag ccatccacgg cggcggcgag ctgcacgtgt gcatgacgcg gaacgcctgc
     4801 gcggggccgg gcggttgcgg ctacctgcgg gtcgaggccg agcggtccgt ccccggcggc
     4861 gccgcgtggc ggcgtgcccg cagggtgttc gagcagcgga tgttcgaggc ggacgcgccg
     4921 cccggcccgg gtctggcgcc tccgggcggg cccgtcccgg gccgtggcgg tgccggggcc
     4981 ggcggaacga cgtccggatc atgacggcgg agtcggcgga gcgggcgccc gcgccccggg
     5041 cccgggcggg cgcgtccccg cgcgaccggc cgggcgcccc gccgggcggc gacacctccg
     5101 ggctgggggt ggggctgcgc tcctcgcacc tggcccagat cctcgccgag tggccggacg
     5161 tcggctggtt cgagatcatc tccgagaact tcatggactc gggcggctac ccgcgccacg
     5221 cgctcggccg catcgccgag cggtacccgg tcgtcatgca cggcgtgtcg atgtcgatcg
     5281 gcagcaccga cccgctcgac ttcggctacc tcgcccggct gcgccggctg gccgactcca
     5341 ccggcgcgcg gtgggtgtcc gaccacgtgt gctggacggg cgtcgcgggc gtcaacaccc
     5401 acgagctgct gccgctcccc ttcaccgagc ggtcgctgcg gcacctcgtc cgccgcgtcg
     5461 gggtcgtgca ggacgtgctg gagcggcggc tcgtcctgga aaacccgagc gcctacgccg
     5521 cgttcgcgca ctcgacgatg cccgagtggg agttcctcgc acggctgtgc gaggagtccg
     5581 ggtgcgggct gctgctggac gtcaacaacg tccacgtctc ggcgttcaac aacggcttcg
     5641 acccggagga gtacatccgc gcgatccccc acgaccgggt cgtccagatc cacgtggccg
     5701 ggcacgccga catgggcgac cacctggtcg acacccacga cggcccggtc gccgagccgg
     5761 tctgggacct ctaccggctg gccacgtccc tgaccgggcc cgtgcccacc ctcctggagt
     5821 gggacgaccg cctcccgccc ctccccgact tactggcgga gctgcacaag gcccgcgacc
     5881 acctcggcgc gcccggtccc gggctgcgtg acgcgggggc ctccgatggg tagcgcgaca
     5941 ccgtccctgg acgcggcgca gcagtggctg ctgcacgccg tcacgacgcc ctccgcctcg
     6001 gcggagcagg ccggcgcgat gctcgcgggc acggacggcc tcgacgcggc gcggcggctg
     6061 gagatctacc gcgtcggcta ccggcgccgc ctgctgggca cgatgcggca catgcttccc
     6121 gcgctgcgcg agctgctcgg ccccgacctg ttcgacgtgt tcgccgccgg gtacctcgac
     6181 gcgcatccgc cgcgcgccta cacgctggcc cggctcggcg accggttcgc cgcctacctg
     6241 gaggagcagc ggcccgaccg cgacctgccc gccggggaac gggagccctg ggccgacctg
     6301 atggtcgacc tcgtccgcta cgagcggctg ttcgccgagg tctacgacgg gccgggcacc
     6361 gaggaggagc cgcccgcgcc ctggcccacc gggctgcccg accgggtgcg cggcgcgccc
     6421 ggcgtccggc tgctgcgggc gtgcgcaccg gtccacgagt accactcggc cgtccggcgc
     6481 ggcgcccgcc cggcgccgcc cgcgttcgtc ccgtcccgca tcgtggtgtt ccgccgcaac
     6541 taccgggtgg tgaccgcctc gccgcccgcg gacgcgttca cgctgctgga ggcgctgatg
     6601 ggcggggcgc cgccggagca ggcgtcggtg cgcgccggac tcgcccccgc cgacgcccgc
     6661 cgccacctgc gccgctggct cgcgcagcgc tggatcaccg cgctgccggc ctcccccgcg
     6721 cgcgagtcgc cgtgacccgc gcggctactc ggcggccagg cgccagtagc cgtgcgggtt
     6781 gaagaagaag tcgtcggcct cgtaccggtc gcaggggtgg tgccgcagga tccagcgggg
     6841 ggcgtccggc gcgagccgga agccctcgcc gtcctcgatc tcctcggcgc ggcgctcggt
     6901 gagcaggtag agggcgagcc cgtcgatgag ggcggcggcc tcgtccgggg acggggaggc
     6961 cgccgggtcc tcgggcggcg cgagttcgat gtcgggcgcg ccgagcaggt gcatgccgca
     7021 gctgtgcagc agcgggccgt cggcgatcgg gcgcttcacc cacgcccggt acagcggcac
     7081 cgccgactcc tcggcgcccc ccgcgtagac gcccgcggcg aggtcgagcc accggtcccg
     7141 gccgtgggtg aggccgctgc tctcgttctt cacggcggtc gcgcccgagc gcagcaggtc
     7201 ggcggtcagc acgagggtcc gccgcgcgac cgtgaacgcg acgcccggca ggacgggcgg
     7261 cgacagcacg taggcgaccg tgtcgtgggc ctcgacggcg tcccagtccg cgcgggtgaa
     7321 ggacgcccgc gccacgcacg cctcgaacgc ctccggcatc cgcgggtcgg cgccggtccc
     7381 ggagtactcc cagtcgagtt cgaagccctc ccgcgccgcc gcggcctcca ccgcgtcgag
     7441 gtcgagcccg gtcccgagga cgcacagcac atgacgggag ctcatcaggg tctggtcgtc
     7501 gatcacgcgg cgcaggttac gcgaggacgg ggtcgcgggc agggtttcgg ccgaaccaga
     7561 ccgttccggt gagatccgta tcaccgctgg tgatccgccc cacatgtcac atttagggcg
     7621 tcccgtccat ctcctgggga gagacccggt gtgccgggtg tgccgagggg gcggggtgcg
     7681 agggatgagt accggtgtgg acgacagcgc gcggcggcgc ttcgatctga cgatgctgca
     7741 cgccgcgtac ggggcgttcc ggcgcgacat ggcggcgatg gccgtcgcgg gccgggtcgc
     7801 gtcctgggga ccgttcacgg ccgagatggg accgttcccg gtcgacgcga acgcctggag
     7861 cctgttcagg ttccactggc tcgcccagca ggcggccgag cggcgcgcgc tgtggacggt
     7921 gatacgcggc cgggtcaccg gcggcaggtc ggcggcgctc gacgcgatgg acgtagaggc
     7981 cgtccggctc gtcgcgctga tggacagggt ggacgccgcg ctggagcacg aggacgacga
     8041 catcctcctc ccgctgaccc gggacctccc ggcggcggtg gaagcgtacc tggacttcaa
     8101 ggaggcgaac gtcctgccgc tgatccacga gaacctcacc ccgtacgagt ggggcaccta
     8161 cgacgtggag ttccgccagg agatcggggt gcgggggctg cgggtgttcc tgccgtggct
     8221 gctggacggc gcgtccgagg cgacgtgcgg cgcggtgctg cggctgctgc cgccgccggt
     8281 caggctcgcc taccggacgc gctggctccc ccgctaccgg cgcgccctcc gccgctgagc
     8341 ggccgcgcac cgcgccggac gcgccactgg cgcgggcgac ggcgcccgtg tgacagcgcc
     8401 cgtgtgacga gtggcacaag ccgtccatgt cacgttcgcc ggtcgagatc tgtcttaggg
     8461 ccagaggctc gacgaaagga ctgtgacgat ggcggggaat cgggcgcccg gaggccgggc
     8521 ggcggacggg cgcgcgggcg ggcgcggctc gggcggggcc gtgggctcgg tgtcccgggc
     8581 gctggacgag cgcttcggca ccgccggttt cgcccgcaag gcgctgcgca aggcgttccc
     8641 ggaccagtgg agcttcctgg tcggagagat cgcgatgtac tcgttcgtga tcatcctgct
     8701 gaccggggtg tacctcacgc tgttcttcaa gccgagcatg cacgaggtca tctacaacgg
     8761 ctcgtacacc aagctgcgcg gcgtgaagat gagcgaggcc tacgcctcgt ccctgaagat
     8821 cagcttcgac gtgcggggcg ggctgctggt ccggcagatc caccactggg cgacgctgat
     8881 cttcatgggg tcgatcctcg tccacctgct gcgcaacttc ttcagcggcg cgttccgcaa
     8941 gccccgcgag ctgaactgga tcatcggcgt caccatgctg gtgctcgtca tggtgaacgg
     9001 gctgttcggc tactcgctgc ccgacgacct gctgtcgggc accggcatcc ggatcctcga
     9061 aggcgtgatc gcggggatcc cgctcgtcgg ctcctacctg gtgatgttcg tgttcggcgg
     9121 cgagttcccc gggtcggaca tcatcccgcg cctgtacgtc gtccacgtcc tgctgatccc
     9181 ggggctgctg gcggcgctga tcccgctgca cgcggtcgtg ctgacctggc ggcagacgca
     9241 cacgcagttc cccggcaagg gcagcacgaa cacgaccgtc cggggctacc cgttcttccc
     9301 ggtgttcatg gccaagacct cggcgctgtt cctgtgggtc ctcggcgtga cgacgctgct
     9361 ggccgcgttc gtccagatca acccgatctg gctgttcggc ccctacgacc cgggcgcgat
     9421 cagcgccgga tcgcagcccg actggtacat gggctggctg gagggctcgc tgcggatcat
     9481 gccggcgtgg gagatcacgg cgtggggcca cacggtgtcg atgagcgtcc tcgtcccggc
     9541 gctggtcgtg cccggggtcc tgttcaccgg cctggccgtc tacccgttcg tcgaacgctg
     9601 ggtgaccggc gaccaccaga tccaccacct tctcgaccgg ccgcgcgacg tccccgcgcg
     9661 cacgggcatc ggcgtcggcg gcatcgtctt ctacgggctg ctgtggctcg cgggcggcaa
     9721 cgacgtcctc gcgaacaagt tcgacgtctc gctgtacgcg acgacgtggt tcttccgcgt
     9781 cgcggtcatc ctcgggccgg tgctgggcta catcgtcgcc taccggatct gcctcgggct
     9841 ccagcgccgc gacctcggcc tcgtcgagca cgggctggag acggggatca tccggcagtc
     9901 gccggacgga cggttcagcg aggtggagcg gcacctgccc gaggagcgga tcgccgcgat
     9961 caccgacccg cgcccggcgc ccgccgtcga cctgaccctc ccgccgccgg tggacggcgt
    10021 cgacgacccg cgcggccgcc acctcaaggg ccgggtgcgg gccgcgctca accgccgttt
    10081 ccgcgcggac ctcgccaccg cgcccgagcg gcccgagccc tccgagcagc gggagctggc
    10141 ggagtccgag cacggtcccc gctgagcggg ccgggctgag cgggccgggc ggcgcgctca
    10201 gcgggcgaac aggcgccgga tcaccgggct ggcggcggcc acgggatcct cgccgaggtc
    10261 gccgctgaac gcgccgctca gccacaactc ggcgaagccg tgcacgatgc accaggcggc
    10321 gagcgcggcc gtccggtcgc cgtccaagcc ggtctcgccg tccaagccgg tctcgccgtc
    10381 caagccggtc tcgccgtcca agccggtctc gtcggtctcg tcgggccgat gggtctcgcg
    10441 gggcgccccg gccggtgcga cggtgcggac gccgtgccgg aggatctcgt ccgcgcgccg
    10501 ccgggcggcg ttcacctcgg ggtcgtcggc ccggtacagc tcgggccgga acatcacctc
    10561 gaagtgcgca cggtggtcga cggcgaagcg gacgtaggcg aggccgacgt cgtagaggtc
    10621 ctcgcccgcc cgctccagcg cgtccgcgaa cagcgtgtac ccctcggcgg cgacggcggt
    10681 gagcagcccg gtcttgtcgc cgaagtggtg ggcgggcgcg gcgtgcgaga cgcccgcccg
    10741 gcgcgccagc tcgcgcaggc tccaggtggc ggggcccgac tcggcgatgg cccccaccgc
    10801 ggcgtgcatg atggcccgcc gcaggtcgcc gtggtgatag ctcggctgct cgatccggct
    10861 cgccccctcg cggtcgcgac gtcccgacgt tcgcccgcca gcctatcttg tcattgacaa
    10921 gttcggggtc gat
//
