LOCUS       NZ_WOFH01000007        10396 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold7, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000007
VERSION     NZ_WOFH01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 452402)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 331232
            Orig. end                         :: 341628
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5396
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    716..730
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     gene            1226..2194
                     /gene="pip"
                     /locus_tag="GNZ18_RS21895"
                     /old_locus_tag="GNZ18_21900"
     CDS             1226..2194
                     /EC_number="3.4.11.5"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (39-301). E-value: 4e-36.
                     Score: 116.8. Matches aSDomain:
                     nrpspksdomains_GNZ18_RS21895_Abhydrolase_1.1"
                     /codon_start=1
                     /gene="pip"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592659.1"
                     /locus_tag="GNZ18_RS21895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21900"
                     /product="prolyl aminopeptidase"
                     /protein_id="WP_156218374.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.3e-11, bitscore:
                     37.5, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 1.2e-32, bitscore:
                     108.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELRTLYPPIEPYDSGLLDVGDGDSVYWEVCGNPEGKPAVMVHGG
                     PGGGCSAAHRRQFDPDSYRIVLFDQRNCGRSLPHAKDPGVSLEANTTWNLVADMERLRE
                     HLGIERWLVFGGSWGSALSLAYAQTHPERVSELVLRGIFTLRPFELYWFYQEGASLLFP
                     DLWESYVEPIPEDEREDLIAAFHERLNSPDRDVRVAAAKAWSTWEGSTVTLRPDPELAA
                     GFAEPDYAVAFARIENHYFVNEGFFEPEQLIRDVDRIRHIPAVIVQGRYDVCTPAATAW
                     DLHRAWPEAEFHLVDDAGHAYSEPGILHRLIEATDRFADAR"
     aSDomain        1343..2128
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GNZ18_RS21895_Abhydrolase_1.1"
                     /evalue="4.00E-36"
                     /label="GNZ18_RS21895_Abhydrolase_1.1"
                     /locus_tag="GNZ18_RS21895"
                     /protein_end="301"
                     /protein_start="39"
                     /score="116.8"
                     /tool="antismash"
                     /translation="VMVHGGPGGGCSAAHRRQFDPDSYRIVLFDQRNCGRSLPHAKDPG
                     VSLEANTTWNLVADMERLREHLGIERWLVFGGSWGSALSLAYAQTHPERVSELVLRGIF
                     TLRPFELYWFYQEGASLLFPDLWESYVEPIPEDEREDLIAAFHERLNSPDRDVRVAAAK
                     AWSTWEGSTVTLRPDPELAAGFAEPDYAVAFARIENHYFVNEGFFEPEQLIRDVDRIRH
                     IPAVIVQGRYDVCTPAATAWDLHRAWPEAEFHLVDDAGHA"
     misc_feature    2216..2230
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    complement(2567..2582)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     misc_feature    complement(2719..2736)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.51"
                     /tool="antismash"
     misc_feature    2813..2827
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            2846..3394
                     /gene="ectA"
                     /locus_tag="GNZ18_RS21900"
                     /old_locus_tag="GNZ18_21905"
     CDS             2846..3394
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657161.1"
                     /locus_tag="GNZ18_RS21900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21905"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_156218375.1"
                     /transl_table=11
                     /translation="MAAQPLESPTRRPERTETNADVQLQEPSLTDGPGLWRIANESHVL
                     DVNSPYSYTLWCRDFADTSVVARDGSALCGFITGYVRPARPDTFFVWQVAVDESYRGRG
                     LARRMLDQLAGRQVARGQRYLEATVTPDNAASTAMFSSFARDHGCDLDRSPLFGTEHFP
                     GRGHEPEVLFRIGPLPGVN"
     gene            3672..4934
                     /gene="ectB"
                     /locus_tag="GNZ18_RS21905"
                     /old_locus_tag="GNZ18_21910"
     CDS             3672..4934
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-357). E-value: 1.4e-74.
                     Score: 243.1. Matches aSDomain:
                     nrpspksdomains_GNZ18_RS21905_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657162.1"
                     /locus_tag="GNZ18_RS21905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21910"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_156218376.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.5e-74, bitscore:
                     245.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVFARMESEVRGYCRGWPTVFTTAKGSHMTDENGRTYLDFFAGA
                     GTLNYGHNNPQLKRRLIDYLAGDAVVHGLDMYTTAKRDFLERFNEYVLAPRGLDYKVQF
                     PGPAGNHSVEAALKLARKYTGRETIVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHG
                     VAMPYDNYLDGRTPDFLLFETMLDDSGSGLDKPAAVIVETIQGEGGINVATAEWLRGLQ
                     DLCRRHDILLIVDDVQMGCGRTGPFFSFEEAGITPDIVCLSKSLSGYGLPFAVTLMRRD
                     LDVWEPGEHNGTFRGFNPAFVTAVAALEEYWLGAGMEKQTLAKGQQVETALNAIALQHA
                     GAIDSVRGRGLAWGLVMSDADLAPKVCEEAFSRGLLMETSGPESEVVKLLPPLTTSEAD
                     LGRGLEIVAESVEAVRSAVAV"
     aSDomain        3735..4742
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GNZ18_RS21905_Aminotran_3.1"
                     /evalue="1.40E-74"
                     /label="GNZ18_RS21905_Aminotran_3.1"
                     /locus_tag="GNZ18_RS21905"
                     /protein_end="357"
                     /protein_start="21"
                     /score="243.1"
                     /tool="antismash"
                     /translation="FTTAKGSHMTDENGRTYLDFFAGAGTLNYGHNNPQLKRRLIDYLA
                     GDAVVHGLDMYTTAKRDFLERFNEYVLAPRGLDYKVQFPGPAGNHSVEAALKLARKYTG
                     RETIVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHGVAMPYDNYLDGRTPDFLLFET
                     MLDDSGSGLDKPAAVIVETIQGEGGINVATAEWLRGLQDLCRRHDILLIVDDVQMGCGR
                     TGPFFSFEEAGITPDIVCLSKSLSGYGLPFAVTLMRRDLDVWEPGEHNGTFRGFNPAFV
                     TAVAALEEYWLGAGMEKQTLAKGQQVETALNAIALQHAGAIDSVRGRGLAWGLVM"
     misc_feature    4991..5006
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.24"
                     /tool="antismash"
     gene            5001..5396
                     /locus_tag="GNZ18_RS21910"
                     /old_locus_tag="GNZ18_21915"
     CDS             5001..5396
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567884.1"
                     /locus_tag="GNZ18_RS21910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21915"
                     /product="ectoine synthase"
                     /protein_id="WP_156218377.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-52, bitscore:
                     171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLEEIIDTERDVRAETWCSRRFVLAKEGVGFSLHETILYAG
                     TETRMWYANHIEAVYCIGGQGELVNEETGEKHRIEPGVMYLLDGHEHHTLRAHTDVRTV
                     CVFNPPCTGREVHDENGIYPLLKEEDA"
     gene            5396..6301
                     /gene="thpD"
                     /locus_tag="GNZ18_RS21915"
                     /old_locus_tag="GNZ18_21920"
     CDS             5396..6301
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630630.1"
                     /locus_tag="GNZ18_RS21915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21920"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_156218378.1"
                     /sec_met_domain="PhyH (E-value: 2e-36, bitscore: 120.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIIESHPTLDDSYPTRKAAEAALLYRQDPVVYGKPQDGPIDSAT
                     LDSFDANGYLSVEELLTPAEVEDYRAELRRMAADPAILADERTVTERGSDEVRSIFEVH
                     RISEVFAALVRDPRVVGRARQILGSDVYVHQSRVNYKPGFTGKDFYWHSDFETWHAEDG
                     MPRMRAVSISIALTENFVHNGGLMIMPGSHKTFVACVGETPSDHYKESLRGQEIGTPDP
                     GSLSILADKHGIELFTGPAGSATMFDCNCMHGSNGNITPFPRSNVFVVFNSVENTCVEP
                     FAAPAPRPSFIGARDFTPVV"
     gene            6396..7289
                     /gene="ehuB"
                     /locus_tag="GNZ18_RS21920"
                     /old_locus_tag="GNZ18_21925"
     CDS             6396..7289
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630631.1"
                     /locus_tag="GNZ18_RS21920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21925"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_156218379.1"
                     /transl_table=11
                     /translation="MTSSRRDFLRSAVLLSAAVPLAAAGCSTTDPKEEKSGGGSLQKAK
                     DAGKITVGFANEAPYGYTDKSGKLTGEAPELARVVFKELGIDKVAGVQVDFSGLIAGLN
                     AKRFDLVAAGMFITPERCNSVAFSDPEYVAKSAFMVRKGNPKGLLKQDDPGKKGVKVGV
                     LEGAVEQGYAEKSGVKKGNIQVFADQASGFEGLKAGRIDCIWLTRISLADLLTKHEGEP
                     YEVTAPFTPVIDGKEQFGAGAFAFRKADSDLVTAFNGKLAELKQGNRLLPIIQPFGFTQ
                     AEIPTAADTAAKFCKG"
     gene            7294..8013
                     /gene="ehuC"
                     /locus_tag="GNZ18_RS21925"
                     /old_locus_tag="GNZ18_21930"
     CDS             7294..8013
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542307.1"
                     /locus_tag="GNZ18_RS21925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21930"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_214617887.1"
                     /transl_table=11
                     /translation="MSEVLDGYEQWLKGAWLTLQLTVYGSGVALVLALVFGLAGTSRHA
                     WIRVLNRVYVEFFRGTATLVLLFWFFYALPLVGIRLNSTMFAAVLALGLNIGAYGAEVV
                     RGSIKAVPRAQYEATIALNFTPLQRMRRVLLPQGFALMLPPFGNLLIELMKGTAIVSLV
                     GLTDLTTVSKNLQSSTGETVASFSLVLVIYFVIAQILQLFVRFAEARVDRMLGRRPSRE
                     PELGTLAAGAPGAGVAS"
     gene            8013..8666
                     /gene="ehuD"
                     /locus_tag="GNZ18_RS21930"
                     /old_locus_tag="GNZ18_21935"
     CDS             8013..8666
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630633.1"
                     /locus_tag="GNZ18_RS21930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21935"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_156218380.1"
                     /transl_table=11
                     /translation="MEWDWSYTGDIIPDLLRGLRYTVIATLLGYLIAVVLGLVWALLRR
                     TPNVVVRQVVRWVTEFIRCTPLIPQLFFAFYVLPTWGLTFGKLTVGVLMLGIHYSTYMA
                     EVYRAGIADVPRGQWEAAKALNLPRTRVWLDVVLPQAVRKVIPTLGNYLIAMFKDSPLL
                     LAIGVPELLTSARSVGARTFEYLEPMTMVGVLFIVVSVISSILLRRLERHYATN"
     gene            8653..9474
                     /gene="ehuA"
                     /locus_tag="GNZ18_RS21935"
                     /old_locus_tag="GNZ18_21940"
     CDS             8653..9474
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657168.1"
                     /locus_tag="GNZ18_RS21935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21940"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_156218381.1"
                     /transl_table=11
                     /translation="MPPTDTTPEKDAAPLRGSAPGIRFEKVIKRFGSNTVLRELDFTVA
                     PGERVTLIGPSGSGKTTILRLLMTLEKPDGGLIFVGDDPLWHMDRGGKRVPANQKHLHE
                     MRKRIGMVFQQFNLFPNMNVLRNLTEGPMRVLGVPRDEAVERAKGLLDLVGLADKIDAH
                     PSQLSGGQQQRVAIARALAMEPQVLLLDEVTSALDPELVVGVQDLLRDIARQSDLTLLC
                     VTHEMTFAKDISDRVLMFDQGQIVEEGPPERMFGEPSEQRTRDFLQAVLAT"
     gene            complement(9584..10237)
                     /locus_tag="GNZ18_RS42370"
                     /old_locus_tag="GNZ18_21945"
     CDS             complement(9584..10237)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621467.1"
                     /locus_tag="GNZ18_RS42370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21945"
                     /product="HD domain-containing protein"
                     /protein_id="WP_156218382.1"
                     /transl_table=11
                     /translation="MGDTFAEFAMPDTDLTRAAYDLAFTAEQRALAHHSVRVYLFGRAL
                     GEARGLRPGDAYDDELLFVACLLHDMGLTDEGDGDQRFEVDGADVAAGFLDGQGVAPDR
                     VRVVWEAIALHTSVGIPQRMRPEIALTSAGAGLDVAGLHADRLPDGYADRVHAAYPRLK
                     KGSGLTHLVVDQIVRKPAKAPFGSLPYEIARQSGVDLKVPSWAEQLERAWSGVA"
ORIGIN
        1 ccgccgacgg cgtcctcgac ggcgcgcagg gcgtcgtcgc gggcctcgct catcgcgtcg
       61 cgctgctgga tcgcctgccg gacggtcgcc tcggcctcgg cgacgcggcg ctcggcctgc
      121 tcgacctgcg cctggagctg ctccatctgg gcgcgggcct gctcggcctg gccgcgggcc
      181 tggtcggcct gcatgcgggc ctgctcggcc tggccgcgga gctggtcggc ctgggtgcgg
      241 ccctgctggg cctggttcca ggcggcggcc gcgtcgcgtt gcgcggcctc cttctcggtc
      301 tgcagcgcgg cgatctgggt gacggcctcg ttctggacct cggcggtgcg gcgctcggcg
      361 tcggcggcct tgcggtcggc ctcgacggcg cggcggtcgg cctgctcggc ctgaccgcgc
      421 gcacgctgca cctcacccca cgcggccgcg gcgtcccgct gcgccgactc cttctccgcc
      481 tgcacggcgg cgagctgcgc ggcggactcg gtctgcgtct cggcgacgcg gcgctcggcc
      541 tccacggcgc ggcggtcggc ctgctcggcc tgaccgcgcg cacgctgcac ctcaccccac
      601 gcggccgcgg cgtcccgctg cgccgactcc ttctccgcct gcacggcggc gacctgggcg
      661 gcggtctcgg tctgcgtctc gctgaccttg cgctcgcccg cggcgagggc gtcgccgatg
      721 aggtcggcca tctggcggag ccgctcgacg atgtcccagg gctgggcctc gggctgctcc
      781 acctccggac gggtggcctc gccgggcttg gacatctgta tcggctgctg ggcggggtcg
      841 cggcccatcg cgggccaggg ggagcccgcg tccgggatcc cgcgcgaacc acccgatccg
      901 ttcagctcac tcactttgtt cctcgcctgg cggctcggag ccgcgttcgc ggggctgcca
      961 tggacatggt gctcgcttca ctcctcgccc tacggctcgt cgcttcgctc acgaaaggca
     1021 ctctagtgcg ctcccgcaga gcgtttgccc agtttggggg tttggattct tgatgctcgc
     1081 gggacgttcg cgcacgcagg acgcctgatc acggttttcg cggtgcgcgt tcggcccggt
     1141 cccacggtgc cacgtgtcac acccccgcga cacgggaacg cgacgaagac gcggtgagga
     1201 agcgctggtg ggacaatcgc gcaccatgga acttcgcacc ttgtatccgc cgatcgagcc
     1261 gtatgactcc gggctgctcg acgtcggcga cggcgacagc gtctactggg aggtctgcgg
     1321 gaaccccgag gggaagccgg cggtgatggt gcacggcggc ccggggggcg ggtgcagcgc
     1381 ggcgcaccgc cgccagttcg acccggactc gtaccggatc gtgctgttcg accagcgcaa
     1441 ctgcgggcgg agcctgccgc acgccaagga cccgggcgtg tcgctggagg cgaacaccac
     1501 ctggaacctc gtcgccgaca tggagcggct gcgcgagcac ctgggcatcg agcggtggct
     1561 ggtgttcggc gggagctggg gcagcgcgct gtcgctggcg tatgcgcaga cgcatccgga
     1621 gcgggtgagc gagctggtgc tgcgcggcat cttcacgctg cggccgttcg agctgtactg
     1681 gttctaccag gagggcgcgt cgctgctgtt ccccgacctg tgggagagct acgtcgagcc
     1741 gatccccgag gacgagcggg aggacctcat cgcggccttc cacgagcggc tgaactcccc
     1801 ggaccgcgac gtccgggtgg cggcggcgaa ggcgtggtcg acgtgggagg ggtcgacggt
     1861 gacgctgcgg cccgaccccg agctggcggc gggcttcgcc gagcccgact acgcggtcgc
     1921 gttcgcgcgg atcgagaacc actacttcgt caacgagggg ttcttcgagc cggagcagct
     1981 catccgggac gtggacagga tccgccacat cccggccgtg atcgtgcagg gccgctacga
     2041 cgtgtgcacg cccgcggcga cggcgtggga cctgcaccgg gcgtggccgg aggcggagtt
     2101 ccacctcgtg gacgacgcgg gccacgccta tagcgagccg ggcatcctgc accggctcat
     2161 cgaggcgacc gaccgtttcg cggacgcccg ctgaatgctc gcccgcaggc ggctggaagc
     2221 aatctgaaaa tctccgggaa gccacctgac cggttccggc aagcctcgat tacggggccg
     2281 ccgcaacgcg ccgggcggtg gatccggaat ggaacggggc cgcgcgttcc cgcgcggccc
     2341 cgatggcgcc gggggacgtc cgaaaaaccg gccgaagcct taccctccgg ccaccaaacc
     2401 cttgcctgga cagatggcac gtctcgtcgc aaggtgagtg tgagacatgc cgggatgccc
     2461 gggttcatac cttgatcccc acccgcccca gcgtggcggg gaccggtccg acctccgcag
     2521 acgcgtgacg acgcccccac acagcggcgc gttgtcagcg aatctcagtg attcacctca
     2581 cacaaccgat acagaattca gctcccccca acccgagtac ttactagagt gacgggcaga
     2641 tgggccgcac gaggggtcgt ccgcccattc ggcagtcccg cctggataac agggggaaat
     2701 gcgcgccgcg gcacggcgtt aacatcgtcg aaatatgtcc gaaattggca agcgtactgc
     2761 catacccatt gcgcgagcgt cctaaacatc tcgcagataa cattccggag cgatcacgcg
     2821 gggcgattcg ctaagttgcc tggacatggc agcgcaaccc ctggaatcac cgacccgacg
     2881 ccccgaacgg accgagacga acgcggacgt gcaactccag gagccgagcc tcaccgacgg
     2941 ccccgggctg tggcggatcg cgaacgagtc ccacgtcctg gacgtcaact cgccctacag
     3001 ctacacgctg tggtgccgcg acttcgccga cacctccgtg gtggcccgtg acggatccgc
     3061 gctctgcggg ttcatcaccg gctacgtgcg gccggcccgt cccgacacgt tcttcgtctg
     3121 gcaggtggcc gtcgacgagt cctaccgcgg ccggggcctc gcccgccgca tgctggacca
     3181 gctcgcgggc cgccaggtgg cccgcggcca gcggtacctg gaggcgaccg tgacgccgga
     3241 caacgccgcc tccaccgcga tgttctcctc gttcgcccgc gatcacggct gcgacctgga
     3301 ccgcagcccc ctgttcggga ccgagcactt tcccggcagg gggcacgagc ccgaggtcct
     3361 cttccggatc ggcccgctcc ccggcgtgaa ctaggcggat gtcccggcac cggcccatcg
     3421 cccgcgcggc caccgccgcg gcgatcgccg acggcccatc acccggacgg atcgcccgca
     3481 ccgatcggcc cgcccgatca gcccgcatcc ccgacccgac cgcccccgac tccgcgcccc
     3541 cgaccgacgg cttgcaccag aacgacggct tgaaccgccc gcgacacgat cgcccacacg
     3601 agatgtccgt ccacccgccc cgttcgtccg cacgcaaccc cgctcccatc cacacctgcc
     3661 ggaggcagac catggacgtt ttcgcccgca tggaatctga ggtccgcggc tattgccgag
     3721 gatggcccac ggtgttcacg accgccaagg gcagccacat gaccgacgag aacggacgca
     3781 cctacctcga cttcttcgcc ggcgccggca cgctgaacta cgggcacaac aacccgcaac
     3841 tgaaacgccg gctcatcgac tacctcgccg gcgacgcggt ggtgcacggg ctcgacatgt
     3901 acaccacggc aaagcgagac ttcctggaac ggttcaacga gtacgtgctc gcgccgcgcg
     3961 gcctggacta caaggtccag ttccccggcc cggcgggcaa ccactcggtg gaggccgcgc
     4021 tgaagctggc gcgcaagtac accggccgcg agaccatcgt gagcttcacc aacgccttcc
     4081 acgggatgac gctcggcgcg ctggccgtca cgggcaactc gatgaagcgc accggcgccg
     4141 gggtcccgct cggccacggc gtggcgatgc cctacgacaa ctacctcgac ggccgcacgc
     4201 ccgacttcct gctgttcgag acgatgctgg acgacagcgg cagcggcctg gacaagcccg
     4261 ccgccgtgat cgtcgagacg atccagggcg agggcggcat caacgtcgcg accgccgagt
     4321 ggctgcgcgg cctccaggac ctgtgccgcc gccacgacat cctgctgatc gtggacgacg
     4381 tgcagatggg ctgcgggcgc accgggccgt tcttcagctt cgaggaggcc gggatcacgc
     4441 ccgacatcgt ctgcctgtcc aagtcgctca gcggctacgg gctcccgttc gcggtcacgc
     4501 tgatgcgccg cgacctggac gtgtgggagc cgggcgagca caacggcacc ttccgcggct
     4561 tcaacccggc gttcgtgacc gccgtcgccg cgctggagga gtactggctg ggcgccggga
     4621 tggagaagca gacgctcgcc aagggccagc aggtcgagac ggcgctgaac gcgatcgcgc
     4681 tccagcacgc cggggcgatc gactccgtcc gcggccgggg cctcgcctgg ggcctggtca
     4741 tgtcggacgc cgacctcgcg cccaaggtgt gcgaggaggc gttcagccgc gggctgctga
     4801 tggagacctc cgggccggag agcgaggtgg tgaagctgct gccgccgctc accaccagcg
     4861 aggccgacct cggccgcggc ctggagatcg tcgccgagtc cgtcgaggcc gtccgctcgg
     4921 ccgtggcggt ctgagccgcg cccgcacggg ccccggcccc atcgacacac cgtcacggct
     4981 tcagcggaaa gaggagattc atgatcgtac gttcccttga ggagatcatc gacaccgagc
     5041 gcgacgtgcg ggccgagacc tggtgcagcc gccgcttcgt cctggccaag gagggcgtgg
     5101 gcttctcgct gcacgagacc atcctgtacg ccggcaccga gacgcggatg tggtacgcga
     5161 accacatcga ggccgtctac tgcatcgggg gtcaggggga actggtcaac gaggagaccg
     5221 gcgagaagca ccgcatcgag cccggcgtga tgtacctgct ggacgggcac gagcaccaca
     5281 ccctgcgtgc ccacaccgac gtgcggaccg tctgcgtctt caacccgccc tgcacgggcc
     5341 gggaggtcca cgacgagaac ggcatctacc cgctgctcaa ggaggaggac gcctgatgag
     5401 catcatcgag tcccatccca cgctcgacga ctcctacccc acccgcaagg ccgccgaggc
     5461 cgccctgctg taccgccagg atcccgtcgt gtacgggaag ccgcaggacg gtcccatcga
     5521 ctccgccacg ctcgactcct tcgacgccaa cggctacctc agcgtcgagg agctgctgac
     5581 gcccgccgag gtcgaggact accgcgccga gctgcgccgg atggccgccg accccgcgat
     5641 cctcgccgac gagcgcaccg tcaccgagcg cggctcggac gaggtccgct ccatcttcga
     5701 ggtccaccgg atcagcgagg tgttcgcggc gctcgtccgc gacccgcgcg tggtcgggcg
     5761 ggcccggcag atcctcggct ccgacgtcta cgtgcaccag agccgggtca attacaagcc
     5821 agggttcact ggcaaggact tctactggca ctcggacttc gagacctggc acgccgagga
     5881 cgggatgccg cgcatgcgcg cggtgagcat ttccatcgcg ctcaccgaga atttcgtgca
     5941 caatggcggg ctaatgatca tgcctggttc gcacaagacc ttcgtggcct gcgtcgggga
     6001 gaccccgtcc gaccactaca aggaatccct gcgcggccag gagatcggca cgcccgaccc
     6061 gggcagcctg tccatcctcg ccgacaagca cgggatcgag ctgttcacgg gcccggcggg
     6121 ctcggcgacc atgttcgact gcaactgcat gcacggctcg aacgggaaca tcaccccgtt
     6181 cccgcgctcc aacgtgttcg tggtgttcaa cagcgtcgag aacacgtgcg tcgagccgtt
     6241 cgccgcaccc gcgccgcgtc cctcgttcat cggggcccgc gacttcacgc cggtcgtctg
     6301 acaacaattc gataaccccg accgatcatc cccgcgcccg cggggggcat ccggcccccc
     6361 gcgggcgcgg cggcacacca tccaggagtt caacgatgac ttcttcgaga cgcgacttcc
     6421 tgcgttccgc cgtcctgctg tccgcggcgg tgccgctggc cgccgcgggc tgctcgacga
     6481 cggaccccaa ggaggagaag tcgggcggcg gttcgctgca gaaggccaag gacgccggca
     6541 agatcacggt cggtttcgcc aacgaggcgc cctacggcta caccgacaag tccgggaagc
     6601 tgaccgggga ggcgcccgaa ctcgcgcggg tggtcttcaa ggaactcggg atcgacaagg
     6661 tggccggcgt ccaggtcgac ttctccggcc tcatcgccgg gctcaacgcc aaacggttcg
     6721 acctcgtcgc ggcgggaatg ttcatcacac ccgagcggtg caattcggtg gccttctccg
     6781 acccggagta cgtcgccaag agcgcgttca tggtccgcaa gggcaacccc aagggcctgc
     6841 tcaagcagga cgacccgggg aagaagggcg tgaaggtggg ggtgctggaa ggcgccgtcg
     6901 aacagggcta tgccgagaag agcggcgtga agaaggggaa catccaggtg ttcgccgacc
     6961 aggccagcgg tttcgagggc ctgaaggccg gccgcatcga ctgcatctgg ctcacccgca
     7021 tctcgctcgc cgacctgctg accaagcacg agggcgagcc gtatgaggtg accgcgccct
     7081 tcacgccggt catcgacggc aaggagcagt tcggcgcggg cgcgttcgcg ttccgcaagg
     7141 ccgacagcga cctcgtgacc gccttcaacg gcaagctcgc cgaactgaag cagggcaacc
     7201 ggctgctgcc gatcatccag ccgttcgggt tcacccaggc ggagatcccg acggccgccg
     7261 acaccgccgc caagttctgc aagggatgat caggtgtccg aagtcctcga cggctacgag
     7321 cagtggctga agggcgcgtg gctcaccctc cagctcacgg tctacggcag cggcgtggca
     7381 ctcgtgctcg ccctggtgtt cgggctggcc ggcacctcgc ggcacgcctg gatccgcgtg
     7441 ctgaaccgcg tgtacgtgga gttcttccgc ggcaccgcga cgctggtgct gctgttctgg
     7501 ttcttctacg cgctgccgct ggtcggcatc aggctgaact ccacgatgtt cgcggcggtg
     7561 ctcgcgctcg ggctgaacat cggcgcctac ggcgccgagg tcgtgcgcgg ctcgatcaag
     7621 gcggtgccgc gggcgcagta cgaggcgacg atcgcgctga acttcacgcc gctccagcgg
     7681 atgcggcgcg tcctgctccc ccaggggttc gcgctgatgc tgccgccctt cggcaacctg
     7741 ctcatcgagc tgatgaaggg cacggcgatc gtgtcgctgg tcgggctgac cgacctgacg
     7801 acggtctcca agaacctgca gagcagcacc ggcgagacgg tcgcgtcgtt ctcgctggtc
     7861 ctcgtcatct acttcgtcat cgcgcagatc ctgcagctct tcgtccgctt cgccgaggcc
     7921 cgcgtcgacc gcatgctcgg ccggcgcccc tcgcgtgagc ccgagctcgg cacgctcgcg
     7981 gcgggcgcgc ccggagcggg ggtggcgagc tgatggagtg ggactggagc tacaccggcg
     8041 acatcatccc cgacctgctg cggggactgc gctacaccgt catcgcgacg ctgctcggct
     8101 acctgatcgc ggtcgtcctc ggcctggtgt gggcgctgct gcgccgcacg ccgaacgtgg
     8161 tcgtgcgcca ggtggtgcgg tgggtcaccg agttcatccg gtgcaccccg ctgatccccc
     8221 agctgttctt cgccttctac gtcctgccca cctggggcct gacgttcggg aagctgaccg
     8281 tcggcgtgct catgctcggg atccactact cgacctacat ggccgaggtg taccgggccg
     8341 ggatcgccga cgtcccgcgc gggcagtggg aggcggccaa ggcgctgaac ctgccgcgga
     8401 cgcgggtgtg gctggacgtc gtcctgccgc aggcggtccg caaggtgatc ccgacgctcg
     8461 gcaactacct gatcgcgatg ttcaaggact cgccgctgct gctggcgatc ggcgtccccg
     8521 agctgctcac ctccgcgcgg tcggtgggcg ccaggacgtt cgagtacctg gagccgatga
     8581 ccatggtggg cgtgctgttc atcgtggtca gcgtcatctc gtcgattctc ctccgccgcc
     8641 tggagaggca ctatgccacc aactgacacc acaccagaga aggacgcggc gcccctgcgg
     8701 ggcagcgccc ccggcatccg cttcgagaag gtgatcaagc ggttcgggag caacaccgtg
     8761 ctccgcgagc tcgacttcac ggtcgcgccg ggcgagcggg tgacgctcat cgggccgagc
     8821 gggtcgggca agaccacgat cctgcggctg ctgatgacgc tggagaagcc cgacgggggc
     8881 ctgatcttcg tcggcgacga cccgctgtgg cacatggacc ggggcggcaa gcgcgtcccg
     8941 gcgaaccaga agcacctgca cgagatgcgc aagcggatcg ggatggtgtt ccagcagttc
     9001 aacctgttcc cgaacatgaa cgtgctgcgc aacctcaccg aggggccgat gcgcgtgctc
     9061 ggggtgccga gggacgaggc cgtcgagcgc gccaagggcc tgctcgacct ggtcgggctc
     9121 gccgacaaga tcgacgcgca cccgtcgcag ctgtcgggcg ggcagcagca gcgggtcgcg
     9181 atcgcgcggg cgctggcgat ggagccgcag gtgctgctgc tggacgaggt gacctccgcg
     9241 ctcgaccccg agctggtcgt cggcgtccag gacctgctgc gcgacatcgc ccggcagagc
     9301 gacctcacgc tgctgtgcgt cacccacgag atgaccttcg ccaaggacat ctccgaccgg
     9361 gtcctgatgt tcgaccaggg gcagatcgtc gaggagggcc cgccggagcg gatgttcggc
     9421 gagccgtccg agcagcgcac ccgggacttc ctccaggcgg tcctggccac ctgagcggcc
     9481 gcgcccggcg ggaccgttcc ggccgggcca tcggaagcat cgcccgcgtc cccgtcgccc
     9541 cgggggcgcg ggcgatcccg tccccggggg cgggagacgc ggctcaggcg acgccgctcc
     9601 acgcccgctc aagctgctcg gcccacgacg ggaccttgag gtccaccccg ctctgccgcg
     9661 cgatctcgta gggcagcgac ccgaagggcg ccttggccgg cttccggacg atctggtcga
     9721 cgaccaggtg ggtgagcccg ctgcccttct tcagccgcgg gtacgccgcg tgcacgcggt
     9781 cggcgtagcc gtccgggagc ctgtccgcgt gcagcccggc gacgtcgagg ccggccccgg
     9841 cgctggtcag cgcgatctcg gggcgcatcc gctgcgggat gccgacgctg gtgtgcaggg
     9901 cgatcgcctc ccacaccacc cgtacccggt ccggcgcgac gccctggccg tcgaggaacc
     9961 cggcggcgac gtcggcgccg tccacctcga accgctggtc gccgtcgccc tcgtcggtca
    10021 gccccatgtc gtgcaggagg cacgccacga acagcagctc gtcgtcgtag gcgtcgccgg
    10081 gacgcagccc ccgcgcctcg cccagcgccc gcccgaacag gtagacccgc acgctgtggt
    10141 gggcgagggc gcgctgctcg gcggtgaagg ccaggtcgta ggccgcgcgg gtgaggtcgg
    10201 tgtcgggcat cgcgaactcg gcgaaggtat cgcccatcga ggtctcctct gatcggttgc
    10261 cacgatcctc ccggcgcttc cggcacccgg ccagtgtccc gagtgcccct gtgcgctacg
    10321 ttctggccat gcatgtggtc gcggtgctcg cgctcgacgg ggtggtggcg ttcgacctgg
    10381 cggtccccgg gcaggt
//
