LOCUS       NZ_WOFH01000007        11884 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold7, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000007
VERSION     NZ_WOFH01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 452402)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 126176
            Orig. end                         :: 138060
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11884
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11884
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11884
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6884](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6884)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    393..407
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            608..1033
                     /locus_tag="GNZ18_RS20995"
                     /old_locus_tag="GNZ18_21000"
     CDS             608..1033
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653747.1"
                     /locus_tag="GNZ18_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21000"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_156218236.1"
                     /transl_table=11
                     /translation="MAARSRLPDDVEPDACAAFRDALDRVGSKWGIAIIAAAGDGPIRF
                     AELHRSIPGISRRMLTLTLRTLERDGLITRTVHPTVPPQVEYTATALAEELFASTRALS
                     AWAENNHAKIAAARAAYDDNEAARHDDRRPVSGPNLY"
     gene            1067..1522
                     /locus_tag="GNZ18_RS21000"
                     /old_locus_tag="GNZ18_21005"
     CDS             1067..1522
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851748.1"
                     /locus_tag="GNZ18_RS21000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21005"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_308166063.1"
                     /transl_table=11
                     /translation="MTDARARGTAAWHGRIEHVRGSDLGSDTVQTSGMRRYEAISGKSV
                     GSERLWMGRTHVGPATSSGDHHHGEAETAIYIVSGHPVFVFADGEDEVRVETAPGDYIF
                     VPPYVPHREENPSPDEEAVVVIARSTQEGIVVNLPSLWADVGRPAES"
     gene            1613..2917
                     /gene="rfbH"
                     /locus_tag="GNZ18_RS21005"
                     /old_locus_tag="GNZ18_21010"
     CDS             1613..2917
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rfbH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571002.1"
                     /locus_tag="GNZ18_RS21005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21010"
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="WP_216651391.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1e-95, bitscore:
                     315.6, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSQDSILELVRKYHREKEEARRFVPGETLVMPSGADLSEDDRV
                     ALVEAALNLRIASGPSAVALERELGRTLRRRKVHLTNSGSSANLLALTALTQPEIGDDR
                     LRPGDEIVTVAAGFPTTVNPILQNQMTPVFVDVELGTYNTTADRVAEAIGPRTRGIMIA
                     HALGNPFPVAEVAELAAERGLFFVEDNCDGVGASYQGRLTGTFGHFSTVSFYPAHHLTM
                     GEGGCVTTDRPRLARIVESLRDWGRDCWCEPGDCDTCGKRYTQRFGELPYGYDHKYTFS
                     HVGYNLKTTDLQAALGLSQLQRLPEFGAARRRNWARLREGLDGLPGLLLPVPTPGSDPS
                     WFGFVITVTADAPFDRDEIVNHLEDRRIGTRMLFAGNLTRQPAYQGRPYRVSGKLDNSD
                     AITERTFWIGVFPGLTEEMVDYMITTIRDFVTSRR"
     gene            complement(2901..4082)
                     /locus_tag="GNZ18_RS21010"
                     /old_locus_tag="GNZ18_21015"
     CDS             complement(2901..4082)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS21010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_21015"
                     /product="hypothetical protein"
                     /protein_id="WP_156218238.1"
                     /transl_table=11
                     /translation="MTAGTPDAAVWDGVSTLLDHYIRIRPDDRVVLLYTADARDPAAWV
                     AAELAVRGVRPALLGMSAGRDDTVEPRLRAALPDPASPHPRIVLLTMERDSMSHVLQLR
                     RALAPYPEDRWATYRVINASAEFFRHSMGVTPELLSALNGALLNRMMPASRLRVTSGGG
                     TDLEIAVDSERYRWISNRGVWRAGAFLVLPPGEVSTYPADVNGVLVADGAFNVTSYTGH
                     DVRLADHPVTVEIEHGRVTGAACADPLVARLLERCLRVENADRIGELGFGTNVGTTAFI
                     PLNSHINERHPGVHLGLGMHGQRLDVVDYWCDVHLDLITADATVAVDGRTEIRSADLRG
                     FAGERHPEPLTGNVHEEDIDGDCCGLITGEVARAREEFLRTRECRRTRATGGS"
     gene            complement(4186..4359)
                     /locus_tag="GNZ18_RS21015"
                     /old_locus_tag="GNZ18_21020"
     CDS             complement(4186..4359)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_21020"
                     /product="hypothetical protein"
                     /protein_id="WP_156218239.1"
                     /transl_table=11
                     /translation="MTVKRPEEAGRESAREEALRAALAEMSTENLATTEGIRVDVVRDD
                     IDAPHERDWGIG"
     gene            complement(4393..5001)
                     /locus_tag="GNZ18_RS21020"
                     /old_locus_tag="GNZ18_21025"
     CDS             complement(4393..5001)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.4"
                     /locus_tag="GNZ18_RS21020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21025"
                     /product="DUF5825 family protein"
                     /protein_id="WP_156218240.1"
                     /transl_table=11
                     /translation="MVPVTMTLWRDYEELDRPGVCAGTAAVSGPAAEASRALFEDGVRR
                     VALKDTVDLSGATDAVPSLVLVRELTAYGIAVDWRVRLGDGRWRDLSHLYPPGEVVGDE
                     EARRDWRDGYHFFKCVYRRGPGFLQVRDRRSGVLRKITIDGPAHRAAVESLLEGCPSAA
                     LPPPVLRDLRTMRLVHFLDGAAWWLPCRVRRWPAGKSVL"
     gene            complement(5001..6884)
                     /locus_tag="GNZ18_RS21025"
                     /old_locus_tag="GNZ18_21030"
     CDS             complement(5001..6884)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="GNZ18_RS21025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21030"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_156218241.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.9e-181, bitscore:
                     597.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVVLVNMPWGSTDVPSLALGILKRAVAERIPEAEVEVLYANIDF
                     VDWVHDRLDMAMDDYDFFAQRSFTEGNGDWVFSSALYDDPDWRRREFDERTSGEKADLA
                     LCHRLHELAPAFVAELAGRVAASAPDVVGFTSTFQQNVAALAAARQVKRLRPETVTILG
                     GASCDGPQGAALHRNFPFVDFVLRGEGEVAFPRFLTAVAGARDFAAIPGLVWRDGSGAS
                     IANAMSARPLAPSEIVAPDYGDYFRRYATSVARLWADPRLVLESSRGCWWGEKHHCGFC
                     GLNGSFMEFRSKPPERFLDEMLALARRHQVLDVYMVDNILDMGYLTSMLPALAAEGYDF
                     RMFYEVKSNLRRQQVRALADAGVVRVQAGIESLSGRVLGIMDKGVTGCQNVRMMRDAES
                     AGVWVEWNYLYGFPGEDAGDYTSVVEQFPALHHLPPPGIVGRLAIERFSPYFDRPELGF
                     AELRPHPQYSMIYDLPEAELFDLAYLFEAPPRGIGAAVAGRVADAAAEWQAAYPESRLT
                     MCDLGDHIDLVNRRPAFGWTALRVEDPVEMAALRLLDRPHRPAALAGKLAAVDPAVTVD
                     GVADLLARWRGLGLIFADGGQVVLTAADAAGQGRRRVRRRAADPADLVGAP"
     gene            complement(7014..7679)
                     /locus_tag="GNZ18_RS21030"
                     /old_locus_tag="GNZ18_21035"
     CDS             complement(7014..7679)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039369.4"
                     /locus_tag="GNZ18_RS21030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21035"
                     /product="DUF6182 family protein"
                     /protein_id="WP_156218242.1"
                     /transl_table=11
                     /translation="MSGPPRDALGAEWDARLERVRLASRAVRGHLPRTAAVAVLRGFTP
                     REFLGSAVAFAAGLPPDRRAAWYGSYSRTIFLAGDPRNLAGRHPCDHLSDDGSIGWYAP
                     APMADREGLRRLLRPFHGPLGVTGPTEEEIPVGEGGGVARLEVPVADLPVEDYLVNVNH
                     LLAEAAMDGLFTGIGRLLVRHLPRDPDERAIRWDRIRVSPDDRSAGTFRAHAYLALSP"
     gene            complement(7676..8515)
                     /locus_tag="GNZ18_RS21035"
                     /old_locus_tag="GNZ18_21040"
     CDS             complement(7676..8515)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS21035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_21040"
                     /product="hypothetical protein"
                     /protein_id="WP_156218243.1"
                     /transl_table=11
                     /translation="MPADRPGPLRLAILGIDGSGKSTVARCLAERSPIGGRHAVLSCVR
                     PHENPDAPLHELSRHLDVLSKEADRLHSPELKLAVLYLQMCTYGVSERFFVRELGADVV
                     ISERHPVIDALAYLPLYRSAIVRLAGPGRADAVREGLAALPAASVEAATAWCEALGRRS
                     GRPAPRLDTLAGELVALPDRPAAEQIAEFRDRFGIGLPDIAVLLDVDVATAADRIGARD
                     RRAEQHERPDRLTRLRDGYERALPAFGSVTIHRIQVGARPPGDVAAEIARLTAAGTR"
     gene            complement(8505..10898)
                     /locus_tag="GNZ18_RS21040"
                     /old_locus_tag="GNZ18_21045"
     CDS             complement(8505..10898)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012588.5"
                     /locus_tag="GNZ18_RS21040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_21045"
                     /product="phosphotransferase"
                     /protein_id="WP_156218244.1"
                     /transl_table=11
                     /translation="MTETIEPGAVEYPALPGRGEYTEEARRARLEWLRAATGAALGSLE
                     TSTIDPRSLTGNVENFVGGIEIPIGLAGPLLFDGQRARGLVTAPLATTEGAMVASVSRG
                     AKAITLAGGVASRVISQRMTRAPVFEFAGLGEAAEFARWVVGRKDALDRQIAAVSTHSR
                     LVELDPVQLGRSVHLRFVYETAEAAGQNMTTVATWRACRWIHDQLAAGSVPVPVWSAIE
                     ANLSGDKKFTRLNMIAGRGTRVVAECVLDEATVRRVLKTTPGAIDRLYRISVVAAQQAG
                     MAGHDIDAANVIAALFVATGQDIACVHESGAGLFSVDVSGGELRATLVLPSLVTGAVGG
                     GTGLAPQRDCLEALGCTGEGGAARFAEITCGFALALNLSTMAAITSGQFADAHERLGRG
                     RRVNWLRPEDLGPALLRPMLAASLDAPGLDVTAVAPIEDAPRSSLITELTSRGERRKTT
                     GVFPLRVTWVRRDGDDPKQTDLVVKVKPLDQEVAIEAAKLASLCGGRLAEVYPRWRDAT
                     GFGGVHTREPAVYRSADPALRAVLPETYGVFEDPAREAHVIVMERLGTGVILKDTVDEP
                     EAWRPRHIAAALRGIASVHAAWLGREEEVRGWLGAARTAESMTAMRELWSAHLEHSAAE
                     HPDLLDACAARRLEDVLDGLPDWWRRMEAMPRTLVHNDFNPRNIALRAADLSLVAYDWE
                     LATLHVPQRDLAELLAFVLSPEASEADVTAYLDLHREAVGAGIDPAAWRDGYRLALWDF
                     ALTRLQMYLMVHTHRELPFLRHVVPTALRLIEIEDAVEEAGRAG"
ORIGIN
        1 ctgaacgggc gcccccggat ctggcacggc agctcctcct cggcggagtc gctggcgctg
       61 gccgccgagt acggcgaccc cgtggtgttc tccaacgcgg gcctgtccag cgagagcgcg
      121 cggccgctgg tcgagtacta ccgcgccgag tgggcgcggc gcggccgtcc cgccgaccgg
      181 ctcttcaccg gcgcggggac gcccgggttc ctcgtcgcca agacctccca ggcggcgctc
      241 gccgcctacc gccccgcctt cgagcgcctc gccgcgatgc agcggagcat cggcgggcgg
      301 ccgccctacg cgagcgtgga ggacttcgtc gcgcgctcgc cggcgctcgt cggcagcccg
      361 gagcaggtca tcgacaaggt gcaccggcag cacgagatct tcggccactc gctcgccgtg
      421 ttcagcgtcg accccgacgg gctgccgctg gacgaccact ggacgtccct cgaactgttc
      481 gccaccgagg tggcgccgac gctgcgcgcc tcgctccccg acccgccgcc ctgacccgcg
      541 cgggacaggt tgccggtgcg gacgatcacg tgtaaggctg agacgtccgc accgggaggg
      601 gagttcgatg gcagcacgat ctcggctacc cgacgacgtc gagcccgacg cgtgcgcggc
      661 cttccgcgac gccctcgacc gggtcgggag caagtgggga atcgcgatca tcgcggcggc
      721 cggcgacggc ccgatccggt tcgccgaact gcaccggtcg atcccgggca tcagccgccg
      781 catgctgacg ctcaccctgc gcaccctcga acgggacggg ctgatcaccc gcaccgtcca
      841 cccgaccgtc ccgccgcagg tcgagtacac cgcgacggcg ctcgccgagg agctgttcgc
      901 ctccacccgc gcgctgtcgg cgtgggcgga gaacaaccac gcgaagatcg cggcggcgcg
      961 cgccgcctac gacgacaacg aggccgcgcg gcacgacgac cggcgccccg tcagcggccc
     1021 caatctctac tgaaccgacc agaaacaacg acaaaagggg cggatcgtga cggacgcgcg
     1081 cgcacgggga acggcggcct ggcacgggcg catcgagcac gtgcgcggct ccgacctcgg
     1141 ctccgacacc gtccagacct ccgggatgcg ccggtacgag gcgatctcgg gcaagagcgt
     1201 cggctcggag cggctctgga tgggccgcac ccacgtcggc ccggcgacca gctcgggcga
     1261 ccaccaccac ggcgaggccg agaccgcgat ctacatcgtc tccggccacc cggtgttcgt
     1321 cttcgccgac ggcgaggacg aggtccgggt ggagaccgcg ccgggcgact acatcttcgt
     1381 cccgccctac gtcccgcacc gcgaggagaa cccctccccc gacgaggagg cggtcgtggt
     1441 catcgcgcgc agcacgcagg agggcatcgt ggtcaacctg ccgagcctgt gggcggacgt
     1501 cgggcgcccc gccgagagct gagtcactaa ggggggccta caggggagga gatcccgcgc
     1561 cccgctgttt tgatggacca ggtccgcgcc gtggcgcgtc ctggaggcga tgatgagtga
     1621 ctcccaggat tcgatcctgg aactggtccg caagtaccac cgcgagaagg aggaggcgcg
     1681 gcggttcgtc cccggcgaga cgctggtgat gccgtccggc gccgacctga gcgaggacga
     1741 ccgcgtcgcg ctggtcgagg ccgcgctcaa cctgcgcatc gcgtccgggc cgagcgcggt
     1801 cgcgctggag cgcgagctcg ggcggacgct gcggcgccgc aaggtgcacc tgacgaactc
     1861 ggggtcgtcg gcgaacctgc tggcgctgac cgcgctcacc cagccggaga tcggcgacga
     1921 ccggctcagg ccgggcgatg agatcgtgac ggtcgcggcg ggcttcccga cgacggtcaa
     1981 cccgatcctg cagaaccaga tgacgccggt cttcgtggac gtggagctcg gcacctacaa
     2041 caccaccgcc gaccgcgtcg ccgaggcgat cggcccgcgc acccgcggca tcatgatcgc
     2101 gcacgcgctg ggcaacccgt tcccggtggc cgaggtcgcc gagctggccg ccgagcgcgg
     2161 gctgttcttc gtcgaggaca actgcgacgg tgtcggcgca agctaccagg ggcggctgac
     2221 cgggacgttc ggccacttca gcaccgtgag cttctacccc gcccaccacc tgacgatggg
     2281 cgagggcggc tgcgtcacca ccgaccggcc gcggctcgcg cggatcgtcg agtcgctgcg
     2341 cgactgggga cgcgactgct ggtgcgagcc cggcgactgc gacacctgcg gcaagcgcta
     2401 cacccagcgg ttcggcgagc tgccctacgg ctacgaccac aagtacacgt tctcgcacgt
     2461 gggctacaac ctgaagacca ccgacctcca ggcggcgctc gggctctcgc agctccagcg
     2521 gctgccggag ttcggcgcgg cccgccgccg caactgggcg cggctgcgcg agggcctgga
     2581 cgggctgccc ggcctgctcc tgcccgtccc gacgccgggc agcgacccga gctggttcgg
     2641 gttcgtgatc acggtcaccg ccgacgcgcc gttcgaccgg gacgagatcg tgaaccacct
     2701 ggaggaccgc cggatcggca cccggatgct gttcgcgggc aacctcaccc gccagcccgc
     2761 ctaccagggc aggccgtacc gggtctcggg gaagctcgac aacagcgacg ccatcaccga
     2821 gcgcacgttc tggatcggcg tcttcccggg cctgaccgag gagatggtcg actacatgat
     2881 caccacgatc cgggacttcg tcacgagccg ccggtagccc gggttcggcg gcactcccgc
     2941 gtccggagga actcctccct ggcacgggcg acctcgccgg tgatgaggcc gcagcagtca
     3001 ccgtcgatgt cctcctcgtg gacgttcccg gtcaggggct cggggtgccg ctccccggcg
     3061 aaaccgcgca ggtccgcgct cctgatctcg gtgcggccgt cgacggcgac cgtcgcgtcc
     3121 gcggtgatga ggtcgaggtg gacgtcgcac cagtagtcga ccacgtcgag gcgctgcccg
     3181 tgcatgccga gcccgaggtg cacgcccggg tggcgctcgt tgatgtggga gttcagcggg
     3241 atgaacgcgg tggtgccgac gttggtgccg aagcccagct ccccgatccg gtcggcgttc
     3301 tcgacccgca ggcagcgctc cagcagccgc gccacgagcg ggtcggcgca cgcggcgccg
     3361 gtgacccggc cgtgctcgat ctcgaccgtg accgggtggt cggccagccg gacgtcgtgg
     3421 ccggtgtagg aggtcacgtt gaacgcgccg tcggcgacga ggacgccgtt gacgtccgcc
     3481 gggtaggtgc tgacctcccc cggcggcagg acgaggaacg cccccgcccg ccacacgccc
     3541 cggttgctga tccaccggta ccgctcggag tcgacggcga tctccaggtc ggtgcccccg
     3601 ccggaggtga cccgcaggcg ggaggcgggc atcatccggt tcagcagcgc gccgttcagc
     3661 gccgacagca gctccggcgt cacgcccatc gagtggcgga agaactccgc cgaggcgttg
     3721 atgacccggt aggtcgccca gcggtcctcg gggtaggggg cgagggcccg gcgcagctgg
     3781 agcacgtgcg acatcgagtc gcgctccatc gtcagcagca cgatccgcgg atgcggggac
     3841 gcggggtcgg ggagcgccgc gcgcagccgg ggctccacgg tgtcgtcgcg gcccgcgctc
     3901 atcccgagca gcgccggcct gacgccgcgg acggccagct cggcggcgac ccacgcggcg
     3961 gggtcccgcg cgtccgccgt gtacaggagg acgacccggt cgtcgggccg gatccggatg
     4021 tagtggtcga gcagggtgga cacgccgtcc cacacggcgg cgtcgggcgt cccggccgtc
     4081 atcgtcatca cctccggcag gcgggggccc cgggtgccgc ccggccggcg gcacccgggg
     4141 tgggcgcaac gcccgcggtc ggccgacccc cgtcggccgc tccggtcagc cgatgcccca
     4201 gtcgcgctcg tgcggggcgt cgatgtcgtc gcggacgacg tccacgcgga tgccctcggt
     4261 cgtggccagg ttctccgtgc tcatctccgc cagcgcggcg cgcagcgcct cctcacgggc
     4321 gctctcgcgc cccgcttcct cgggtctctt cacggtcatg ccgcacctca tctctccgga
     4381 cacttccact gatcacagga cgctctttcc ggcgggccac cggcgcaccc ggcagggcag
     4441 ccaccaggcc gcgccgtcga ggaagtggac gagccgcatc gtcctcaggt cgcgcaggac
     4501 cgggggcggg agcgccgcgg acgggcagcc ctccagcagg ctctcgaccg ccgcgcgatg
     4561 cgccgggccg tcgatggtga tcttgcgcag gacgcccgaa cgccggtcgc gcacctggag
     4621 gaatccgggt ccgcggcggt agacgcactt gaagaagtgg tagccgtccc gccagtcgcg
     4681 gcgggcctcc tcgtcgccga cgacctcgcc gggcgggtag aggtggctca ggtcccgcca
     4741 ccggccgtcg cccaggcgca cccgccagtc gacggcgatc ccgtatgcgg tcagctccct
     4801 taccagtacg aggctgggga cggcgtcggt cgcgcccgaa aggtccacgg tgtccttcag
     4861 cgcgacgcgg cgcacgccgt cctcgaagag ggctcggctc gcctcggcgg cggggccgga
     4921 gacggcggcg gtcccggcgc acacccccgg ccggtcgagt tcctcgtagt cgcgccacag
     4981 cgtcatcgtg accgggacca tcacggcgct ccgaccaggt cggccgggtc ggcggcgcgc
     5041 cggcgcaccc gccgccggcc ctggcccgcc gcgtcggcgg cggtcaggac gacctggccg
     5101 ccgtcggcga aaatcagccc gaggccgcgc cagcgcgcga gcaggtccgc gacgccgtcc
     5161 acggtgacgg ccgggtccac cgcggcgagc ttcccggcca gcgccgccgg gcggtgcggc
     5221 cggtcgagca ggcgcagggc ggccatctcc acgggatcct cgacccgcag cgccgtccag
     5281 ccgaaggccg ggcgccggtt caccaggtcg atgtggtcgc cgaggtcgca catggtcagc
     5341 cggctctcgg gataggccgc ctgccattcg gcggccgcgt cggcgacgcg tccggcgacc
     5401 gccgccccga ttccgcgcgg cggcgcctcg aagaggtagg cgaggtcgaa cagctccgct
     5461 tcgggcaggt cgtagatcat cgagtactgg ggatggggcc gcagttccgc gaagcccaac
     5521 tcgggccggt cgaagtacgg gctgaaccgc tcgatcgcga gcctgccgac gatgcccggc
     5581 ggcggcaggt ggtgcagcgc ggggaactgc tcgacgaccg acgtgtagtc gccggcgtcc
     5641 tcgcccggga agccgtagag gtagttccac tccacccaga cgccggccga ttcggcgtcg
     5701 cgcatcatcc ggacgttctg gcagccggtc acgcccttgt ccatgatgcc gaggacgcgg
     5761 ccgctgaggc tctcgatgcc cgcctggacc cgcacgacgc ccgcgtccgc cagcgcccgc
     5821 acctgctgcc gccgcaggtt ggacttgacc tcgtagaaca tgcggaagtc gtagccctcg
     5881 gcggcgagcg cgggcagcat cgacgtcaga tagcccatgt cgaggatgtt gtcgaccatg
     5941 tacacgtcga gcacctggtg ccgccgggcc agggcgagca tctcgtccag gaaccgttcc
     6001 gggggcttgc tgcggaattc catgaacgac ccgttcagac cgcagaagcc gcaatggtgc
     6061 ttctcgcccc accagcagcc gcgcgagctt tccaggacga gccgcggatc ggcccacaac
     6121 cgcgcgacgg acgtggcgta ccgccggaag tagtcgccgt agtccggggc gacgatctca
     6181 ctcggcgcca gcggccgcgc gctcatggcg ttggcgatcg aggctccgct cccgtcccgc
     6241 cagacgaggc cgggaatcgc cgcgaagtcg cgggcccccg ccacggcggt gaggaatcgc
     6301 ggaaaggcga cctcgccctc gccgcgcagg acgaagtcca cgaacgggaa gttgcggtgc
     6361 agcgcggcgc cctgcggccc gtcgcagctc gccccgccga ggatcgtcac ggtctccggc
     6421 cggagccgct tgacctgcct ggccgccgcg agcgcggcga cgttctgctg gaaggtggag
     6481 gtgaagccga ccacgtcggg cgcggacgcg gcgacgcgtc cggcgagctc ggcgacgaac
     6541 gcgggcgcca gctcgtgcag ccggtggcac agggccaggt cggccttctc cccgctcgtc
     6601 cgctcgtcga actcgcgccg ccgccagtcc ggatcgtcgt agagcgccga ggagaagacc
     6661 cagtcgccgt tcccctcggt gaaggaccgc tgggcgaaga agtcgtagtc gtccatcgcc
     6721 atgtcgaggc ggtcgtggac ccagtcgacg aagtcgatgt tggcgtacag cacctcgacc
     6781 tcggcctcgg ggatccgttc cgccacggcg cgtttgagga tgcccagggc cagcgaggga
     6841 acgtcggtgg agccccacgg catgttgacg agaactaccc gcatcgcgct cccctcgggg
     6901 gtgcggccac gatcccggcg tcacgcccat ggtccgccgg gccgccggga ccggcaaggc
     6961 cgcgtccgta ggtggacacg cccgatacgg agagtcgcgg gacccctctg gcgttacggg
     7021 ctgagggcca ggtaggcgtg ggcacggaac gtgcccgccg accggtcgtc gggcgagacc
     7081 cggatgcgat cccaccggat ggcgcgctca tcgggatcgc ggggaagatg ccggacgagc
     7141 agccgcccga tcccggtgaa cagcccgtcc atggccgcct cggcgaggag gtggttgacg
     7201 ttgacgaggt agtcctcgac cggcaggtcc gccaccggga cctccagccg cgcgaccccg
     7261 ccgccttcgc cgaccgggat ctcctcctcg gtcgggccgg tgacgccgag gggcccgtgg
     7321 aagggcctga gcagtcgccg cagaccctcc cggtccgcca tcggggcggg ggcgtaccag
     7381 ccgatggaac cgtcgtcgga aaggtggtcg caggggtggc gtccggcgag gttgcggggg
     7441 tcgcccgcga ggaagatcgt cctgctgtag ctgccgtacc aggcggcgcg ccggtccggc
     7501 ggcagcccgg cggcgaaggc gaccgccgaa ccgaggaact cgcgcggggt gaacccgcgc
     7561 aggacggcga cggcggcggt gcgcggcagg tggccgcgca ccgcgcgcga cgcgaggcgg
     7621 acgcgttcca gccgggcgtc ccattccgcg ccgagcgcgt cccgcggcgg cccgctcatc
     7681 gcgtcccggc ggcggtgagc cgcgcgatct cggcggcgac gtcccccggc gggcgggcgc
     7741 cgacctggat ccggtggatc gtcaccgacc cgaacgcggg gagcgcccgc tcgtacccgt
     7801 cgcggagccg ggtgagccgg tcggggcgct cgtgctgctc ggcgcggcgg tcgcgggcgc
     7861 cgatccggtc ggcggcggtc gcgacgtcga cgtcgagcag cacggcgatg tccggcaggc
     7921 cgatcccgaa ccggtcccgg aactcggcga tctgctcggc cgccgggcgg tccggcagcg
     7981 cgaccagctc accggcgagc gtgtccagcc gcggcgcggg ccgcccggag cgccgtccga
     8041 gcgcctcgca ccaggcggtc gcggcctcga ccgacgcggc gggcagcgcg gccagcccct
     8101 cccggacggc gtccgcgcgg ccgggcccgg cgaggcggac gatcgcgctc cggtacagcg
     8161 gcaggtaggc gagcgcgtcg atgacggggt ggcgctcgct gatgacgacg tccgccccca
     8221 gctcgcggac gaagaaccgc tcgctgaccc cgtaggtgca catctggagg tagaggacgg
     8281 ccagcttcag ctccgggctg tggagccggt cggcctcctt ggacaggacg tcgaggtgcc
     8341 gcgacagctc gtgcagcggc gcgtcggggt tctcgtgggg gcgcacgcac gacaggacgg
     8401 cgtgccgccc gccgatgggc gaccgctccg cgaggcaccg ggcgaccgtg ctcttgcccg
     8461 agccgtcgat cccgaggatc gccagccgca gcggaccggg acggtcagcc ggcacggccc
     8521 gcctcctcca cggcgtcctc gatctcgatc agccgcagcg ccgtggggac gacgtgccgc
     8581 aggaacggca gctcgcggtg ggtgtgcacc atcaggtaca tctggagccg cgtgagggcg
     8641 aagtcccaca gggcgaggcg gtacccgtcc cgccaggcgg ccgggtcgat cccggccccg
     8701 accgcctccc ggtggaggtc gaggtaggcg gtcacgtccg cctcggacgc ctccggcgag
     8761 agcacgaacg cgagcagttc ggcgaggtcg cgctgcggga cgtggagcgt cgccagctcc
     8821 cagtcgtagg cgaccaggct caggtcggcg gcgcgcagcg cgatgttgcg cgggttgaag
     8881 tcgttgtgga cgagcgtgcg cggcatcgcc tccatccggc gccaccagtc cggcagcccg
     8941 tcgaggacgt cctccagccg ccgcgcggcg caggcgtcca gcaggtccgg gtgctcggcc
     9001 gcgctgtgct ccaggtgcgc cgaccacagc tcgcgcatgg ccgtcatgct ctcggccgtc
     9061 cgggcggcgc cgagccagcc gcgcacctcc tcctcgcgcc cgagccaggc ggcgtggacg
     9121 gacgcgatgc cccggagggc ggcggcgatg tgccgcggcc gccacgcctc cggctcgtcg
     9181 acggtgtcct tgaggatcac gcccgtgccg agccgctcca tcacgatcac gtgggcctcg
     9241 cgcgccgggt cctcgaagac gccgtaggtc tcgggcagga cggcgcgcag cgcgggatcg
     9301 gccgaccggt agacggcggg ctcccgggtg tgcacgccgc cgaacccggt cgcgtcgcgc
     9361 cagcgcgggt agacctcggc gaggcggccg ccgcacagcg aggccagctt cgccgcctcg
     9421 atggccacct cctggtcgag cggcttcacc ttgacgacca ggtccgtctg cttggggtcg
     9481 tcgccgtccc gccggaccca ggtgacgcgg agcgggaaca cccccgtcgt cttgcgccgc
     9541 tccccgcgcg aggtcagctc ggtgatgagg ctcgaccggg gggcgtcctc gatcggggcg
     9601 acggcggtga cgtccagccc gggggcgtcg agcgacgcgg cgagcatcgg ccgcagcagc
     9661 gccgggccga ggtcctcggg acgcagccag ttcacccgcc gcccgcggcc gagccgctcg
     9721 tgcgcgtcgg cgaactggcc gctggtgatc gccgccatcg tcgagaggtt gagcgcgagc
     9781 gcgaacccgc aggtgatctc cgcgaagcgc gcggcgccgc cctcgccggt gcagccgagc
     9841 gcctccaggc agtcgcgctg cggcgcgagg cccgtgccgc cgccgaccgc gccggtgacc
     9901 aggctcggca ggacgagcgt cgcccgcagc tcgccgccgg agacgtcgac cgagaacagc
     9961 ccggcgccgg actcgtggac gcaggcgatg tcctggccgg tcgcgacgaa cagcgccgcg
    10021 atcacgttgg cggcgtcgat gtcgtgcccg gccatgcccg cctgctgcgc ggcgaccacg
    10081 ctgatccggt agagccggtc gatggcgccc ggggtggtct tgaggacgcg gcggaccgtc
    10141 gcctcgtcca gcacgcactc ggccacgaca cgggtgccgc gtccggcgat catgttcagc
    10201 cgggtgaact tcttgtcgcc gctcaggttc gcctcgatcg ccgaccacac cggcacgggc
    10261 accgacccgg cggccagttg gtcgtggatc caccggcagg cccgccaggt cgcgaccgtc
    10321 gtcatgttct gcccggcggc ctcggccgtc tcgtacacga accgcaggtg gacggaccgg
    10381 ccgagctgca ccgggtccag ctcgaccagc cgcgagtgcg tggacaccgc cgcgatctgc
    10441 cggtcgagcg cgtccttgcg gccgacgacc cagcgggcga actccgccgc ctcgccgagg
    10501 cccgcgaact cgaacaccgg cgcccgggtc atccgctgcg agatgacccg gctcgccacc
    10561 ccgcccgcca gcgtgatcgc cttcgcgccg cgcgacaccg aggcgaccat ggcgccctcg
    10621 gtggtggcga gcggcgccgt gaccaggccg cgcgcccgct gcccgtcgaa cagcagcggc
    10681 ccggcgaggc cgatcgggat ctcgatgccg ccgacgaagt tctccacgtt cccggtcagc
    10741 gagcgggggt cgatcgtcga ggtctccagc gagccgagcg cggcgcccgt cgccgcccgc
    10801 agccactcca gccgggcccg ccgcgcctcc tcggtgtact cgccgcgccc ggggagggcg
    10861 gggtactcga cggcgccggg ttcgatggtc tcagtcacgg gtcgtgctcc cctcgtcctg
    10921 ggtgcccggg gcggcggtca cccgcgccca gacctcgggg tcgtcctgga acacccggtc
    10981 cttcacgcgc ggcggccgct cccccgcctg gacgcggcgc cgccactccc gccagaaccc
    11041 ctcggggtcc accgccctga ccagcggcgg cgcgagggcc tcggcgtcgc gggcggcggc
    11101 gtaggcgggc gactccacgc gcagggcccg gtcgagggcc gcgccgaacg ccgccgcgcc
    11161 gggcccggcc ggcggctcac ggaaggcgac cgcgacctcg tgccgaggat gctcggcgtc
    11221 ggccctgcac aggacgttgc ggacgcccag tcccgtcgcc tcggccgccg ccgccagcgc
    11281 gcggagcatc tgcgactcgc gcagccgctc ccccgcgacc gagagcagcc ccttgcggca
    11341 cgcgtactcc acgaccgggg tcgcctcgac gaacccggtc accttcacga tgtcgcggac
    11401 cgcgcatcgg tagagcccgc cgagatggga gaggatcacg tggtactcgc gcccctcctc
    11461 caactcgtgc gccgacagcg tcgggccgcg cgggtcgatc tcctcctcgg ccgggacgaa
    11521 ctcgtagaag cagctcgtca cgaccagggg gccgcccgcc ggacggtcgt ccaccgggac
    11581 ggcgagcggc ccctccgacg acccgatcgg cgcggggagc acccggacct tcgggccgta
    11641 gtcgtcgagg aggccgggga ggtagagccg ggagagatag gtgttccagg tcaggaggag
    11701 ctccagccgg ggccagaggt ccaccgggcg gggcccgccg ccgcgccggg cggcgcgctc
    11761 cagctcgtcg gcccgcgccg ggttcggctc ccggaacggg gagccgccga gcgtcccgtc
    11821 cctgatctcc gcgaccatcc gggcccacca gtgccgcagc tgccggggca gcgcggcggc
    11881 gatg
//
