LOCUS       NZ_WOFH01000006        11317 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold6, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000006
VERSION     NZ_WOFH01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 632247)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 294828
            Orig. end                         :: 306145
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11317
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11317
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11317
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6317](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6317
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            232..900
                     /locus_tag="GNZ18_RS18955"
                     /old_locus_tag="GNZ18_18960"
     CDS             232..900
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290588.1"
                     /locus_tag="GNZ18_RS18955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18960"
                     /product="HD family hydrolase"
                     /protein_id="WP_156217829.1"
                     /transl_table=11
                     /translation="MTSTTRRDLIGRAEPPRDVPERLASQIAFIAEVDRLKTVVRRSPL
                     LAADRRENDAEHSWHLALMVTVLAEYADEPIDVARTLRLVLVHDLVEIYAGDTFLYDEA
                     ASADQAEREEAAADELFALLPPDQAGAFRALWDEFEARATPEARFAKAMDRLQPLLLNF
                     GNEGGTWHTPGVTEADVRRRKSVIGDASAELGAYASKLIDLGAQRGWLRRDGGGDGVGS
                     "
     misc_feature    complement(926..941)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.89"
                     /tool="antismash"
     gene            1068..1412
                     /locus_tag="GNZ18_RS18960"
                     /old_locus_tag="GNZ18_18965"
     CDS             1068..1412
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS18960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_18965"
                     /product="hypothetical protein"
                     /protein_id="WP_156217830.1"
                     /transl_table=11
                     /translation="MDSDVRVAVCGAGLRVWFPWPARACSVVDVRGLPARASVGKGLTI
                     GLDGLPENVMGLLARVETRAAEIDVAGAALRVHVPGGHAEFQMEGESTVALRLCGRELV
                     GIDVRFLGRP"
     gene            1481..2146
                     /locus_tag="GNZ18_RS18965"
                     /old_locus_tag="GNZ18_18970"
     CDS             1481..2146
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019350.5"
                     /locus_tag="GNZ18_RS18965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18970"
                     /product="sensor histidine kinase"
                     /protein_id="WP_156217831.1"
                     /transl_table=11
                     /translation="MDRREKNGKVAWELTAGIEEERQRLRREIHDQMAPALAALRLRFD
                     LAREVVADGAGGPDPLDSLDSVDEAIRNLGEALSGLLAAIDGAFPAELARLGLIGALAD
                     LGAFLSLADLRIEADENELLEMSPACEVAAYKIAAEALTNARKYSSDRSAVVRIDRQDG
                     DIVLQVSSAIGSERERHPSYGVGFRSMRERAAALGGKCNVRATDEGMVVRARIPSRPA"
     gene            2143..2799
                     /locus_tag="GNZ18_RS18970"
                     /old_locus_tag="GNZ18_18975"
     CDS             2143..2799
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="GNZ18_RS18970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18975"
                     /product="response regulator transcription factor"
                     /protein_id="WP_173390822.1"
                     /transl_table=11
                     /translation="MSVPVLIVEDHPLYLGALRALFERGDGFEVVAEAATGAAALEALE
                     GPRGARPRVITLDLHLPDGSGTALAQRVLAAHPQVRVLVISALTDRAAVLSALRAGVHG
                     YLTKDAGSEEIRHAVRLVAAGGTAFGEPIAVLLRDWLKADAGASARTHFPTLTGRETQV
                     LDLLAGGREYAQIARELGISEKTVRNHLSNLFVKLEVNNRAQAILVARRAGMGTS"
     gene            complement(2848..3405)
                     /locus_tag="GNZ18_RS18975"
                     /old_locus_tag="GNZ18_18980"
     CDS             complement(2848..3405)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /locus_tag="GNZ18_RS18975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18980"
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="WP_156217833.1"
                     /transl_table=11
                     /translation="MTDLFRLDATALALIGHDVALIPDAAMADPTPCEGWTVADLLHHM
                     NERHEALARTVLPPLEPGEDPRDDFARVAGRSLTALDRTGATVDLPWRGPAPTEWVLGV
                     HVVDMLVHRWDLARALGTALDTPAGLLRAALPLARANTAPGSVLNGPGGVYLPPLPEDS
                     GRSAIDTIVALLGRDPRWTPPA"
     gene            3477..4079
                     /locus_tag="GNZ18_RS18980"
                     /old_locus_tag="GNZ18_18985"
     CDS             3477..4079
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540668.1"
                     /locus_tag="GNZ18_RS18980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18985"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_308165975.1"
                     /transl_table=11
                     /translation="MSPRHSIAEAARTRDRIVRAAVRDASRLGLEGLTVGSLARRLGMS
                     KAGLIGPFGSREALLAAALDHAVQIFQAAVIAPLEQRPPGAERLELLIDRWVAYLADCP
                     FPGGCFVTAASSELDGRPGPLRDRLREGVASWRARIAAEVAAAQAELPGPHRPAEEVAV
                     TLVGLSMALNQEVQLLEDREAAPRARAAMRHAAGLRP"
     gene            4223..4771
                     /locus_tag="GNZ18_RS18985"
                     /old_locus_tag="GNZ18_18990"
     CDS             4223..4771
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012859.5"
                     /locus_tag="GNZ18_RS18985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18990"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_173390823.1"
                     /transl_table=11
                     /translation="MKRTTRVLGAALALLFVGVGNGTAFAQPAQPAQPAQPAQPAKTTR
                     TAKAADAKILAVRHFYNDRFQRCLDADTNTGGNGGIVQLWDCGPWNQQDWEVWPDGHIY
                     NSRFQKCLDADTNTGGNGGKVQLWGCGPWNQQWWDIWPDGHLYNRRFQRCLDADTNTGG
                     NGGIVQLWDCGPWNQQWWR"
     misc_feature    4858..4871
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.95"
                     /tool="antismash"
     misc_feature    complement(4859..4872)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.82"
                     /tool="antismash"
     gene            5001..6317
                     /locus_tag="GNZ18_RS18990"
                     /old_locus_tag="GNZ18_18995"
     CDS             5001..6317
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.5"
                     /locus_tag="GNZ18_RS18990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_18995"
                     /product="YcaO-like family protein"
                     /protein_id="WP_173390824.1"
                     /sec_met_domain="YcaO (E-value: 3.5e-38, bitscore: 127.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQDRYQRSGPLPREGALWCADHFRGARGFRAAAPHAGERAFPL
                     PRARALIEAALDEAALRPRLTDLRGAWRCHLSHPDGTPVRDGVGAGKGDPQSAQVGALY
                     EALEHHRGAALPARGEPVELLTAAQLAGTDLARDPALEPLAASGGPGAAACRRYTALDH
                     SGRTLPVPVFLTNPAYLECPADRRHELGDVLDYAPLSRFSTNSGTAIGATRDEALVHAL
                     AEAVERDAFSLLLAGTFLLARPSPLRLVRPASLPDHLQELYLSAGWHAGGSVALIDMTS
                     DLGVPAFCAHTSRDGRAAQGQGASLSAEHAVRRALNELIQVVAIRRGERNPPADLRAVE
                     SHPRLLEAARADFDPHLAGARPRDFLPTGAPAAPREHLQRLTATLTGYGYRPYGRVLRT
                     EPNGVTTVSVFVPGLERFFAVTAGACVVPGPRGRALGPG"
     gene            6314..7261
                     /locus_tag="GNZ18_RS18995"
                     /old_locus_tag="GNZ18_19000"
     CDS             6314..7261
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="GNZ18_RS18995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_19000"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_156217836.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.3e-23, bitscore:
                     78.7, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSHDVVVVGGGPAGLSAALVLARACRAVAVVDAGRPRNAASAHV
                     HGVLGLPDLSPQVLLEVGRAQVLAYGVDVLEGTARTARPRPEGGFAVTLSDGRVLAGAH
                     LLLATGTVDELPDIPGLAPRWGRDAVACPHCHGWELRGRAIAILGGGRADARRALMWRQ
                     WTPNLTLITAPDHPLAPSDLERLGARGITVLDGPVTEVIAAADRLCGVRLRDGTSVPIR
                     ALVVAPCFRARADLLDGLGLRPTELTVGGRVVGTVIATDGAGRTAVPGVWAAGDGASPL
                     ERVVAVTADGAAVASAINAALTDGSHPEHSAGEP"
     misc_feature    7282..7291
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    complement(7282..7296)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.86"
                     /tool="antismash"
     misc_feature    complement(7283..7296)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.4"
                     /tool="antismash"
     misc_feature    complement(7284..7297)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.52"
                     /tool="antismash"
     gene            7411..8520
                     /locus_tag="GNZ18_RS19000"
                     /old_locus_tag="GNZ18_19005"
     CDS             7411..8520
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS19000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_19005"
                     /product="hypothetical protein"
                     /protein_id="WP_156217837.1"
                     /transl_table=11
                     /translation="MRHGLIRVVASIGAVLAVASTGHAVAAPAGPGPVLVDEPFTRATA
                     DSRFIGYGAACLTGAARGNAPQEGANHALSGCRRDPAGPVPPGGAAPRGYLQLTDAHPE
                     VTGAVMFDSPVPARDGLEVTFEQWQYGNTTRAPADGISFFLTDGARSLHTPGAFGGSLG
                     YAQKLPGGRSDAHFVPGVSGGYLGLGFDVLGEYFGDGERRGNGCERRSPAGTEFRAPAP
                     GPNIVTARGPGNGIDGYCLLDATTSNRSSTGPWTSSLPGRLHGDLRTMPARVTAKKAEG
                     LLEPVKRVVRVVVTPAPNPVVTVDIDFQDDEGFQRVLRFPAPQPVPAAYRFGFAASTGP
                     LTDVQLIRKVVLRAQRPAGDEEPSESEDE"
     gene            complement(8578..9090)
                     /locus_tag="GNZ18_RS19005"
                     /old_locus_tag="GNZ18_19010"
     CDS             complement(8578..9090)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS19005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_19010"
                     /product="hypothetical protein"
                     /protein_id="WP_156217838.1"
                     /transl_table=11
                     /translation="MSFSPEVLPAAVKAITNSPSRQRPVVMITCGADGLPQVCVPDWGE
                     LRVVGDRHLRVSVWPTDPTAVNLDRDAPALLIVTALSDVYLVHATARRLPGAAMVWAHY
                     ELTITSARIADRGTTPRLVPDHDTPTEFPAQRLPRARDMLGTKVTALRAHRRDPGHYRG
                     DLGDLRL"
     misc_feature    complement(9174..9189)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 25.17"
                     /tool="antismash"
     misc_feature    9175..9188
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            complement(9341..9823)
                     /locus_tag="GNZ18_RS19010"
                     /old_locus_tag="GNZ18_19015"
     CDS             complement(9341..9823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542531.1"
                     /locus_tag="GNZ18_RS19010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_19015"
                     /product="iron chaperone"
                     /protein_id="WP_156217839.1"
                     /transl_table=11
                     /translation="MSETPKAAKGDAATGKNFDGFSEDERAAMKEHAKELKKAARRSAS
                     EAKAREEGERDVREKIAEMPEADRVLAERLHEIIASVAPGLVPRTWYGMPAYAKDGKVL
                     CFFQSAQKFKSRYATLGFSDVAALDDGTLWPTTYALTALDAANEAVISDLVKRAVG"
     gene            complement(9892..10347)
                     /locus_tag="GNZ18_RS19015"
                     /old_locus_tag="GNZ18_19020"
     CDS             complement(9892..10347)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891852.1"
                     /locus_tag="GNZ18_RS19015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_19020"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_156218094.1"
                     /transl_table=11
                     /translation="MQSRMQNPAAVLTGAMRPIQDLFKAAHSGGVDQQTLELVHLRASQ
                     INGCSPCVDSGVKSARKAGVGDDKLALVAAWREAPYFTDEERAALALAEAATRLADRPD
                     AVDDETWDAAATYYDEKQLAAIVLMIALTNLFNRLNATTRQIAGAWG"
ORIGIN
        1 ttcgaccttg tcgtcgaccc cggactggtg gtccgctgcc acggcggccc ccttcctcgt
       61 ccgctctgga cgcttcgggc aaagttcacc gccttcacag cccgcgtact tgcctgtgtc
      121 ggaacagtgc ttgtctgtcc cggaacggct cggtgtcgat catgcgttga ccgggtagcc
      181 gcgaaccacg ggagaccgcg cggccccggg tccccgaccg aaaggatctc catgacctcc
      241 acgacgcggc gcgacctcat cggcagggcc gagcccccgc gcgacgtccc ggagcggctc
      301 gcgtcccaga tcgcgttcat cgccgaggtg gaccggctga agaccgtcgt gcggcgcagc
      361 ccgctgctgg ccgccgaccg gcgcgagaac gacgccgagc actcctggca cctcgcgttg
      421 atggtcaccg tgctcgccga gtacgccgac gagccgatcg acgtcgcgcg gacgctgcgg
      481 ctcgtcctgg tgcacgacct cgtggagatc tacgcgggcg acaccttcct ctacgacgag
      541 gccgcgtcgg ccgaccaggc cgagcgggag gaggccgcgg ccgacgagct gttcgcgctg
      601 ctgccgcccg accaggccgg ggcgttccgc gcgctgtggg acgagttcga ggcccgcgcg
      661 accccggagg cgcggttcgc caaggccatg gaccgcctcc agccgctgct gctcaacttc
      721 ggcaacgagg gcggcacctg gcacaccccg ggcgtcaccg aggccgacgt ccgccgccgc
      781 aagtcggtga tcggcgacgc gtccgcggag ctgggcgcct acgcgagcaa gctcatcgac
      841 ctgggcgccc agcgcggctg gctccgccgc gacggcggcg gggacggcgt gggttcctga
      901 cgccggccgg gcgccgatcc tcctgggtgg tcgagacctt cagcccatgc ccccgggtga
      961 ccgggcatgg ttcggtgggc cctgacacgg actcggccga aggagcggac cattacgcgg
     1021 caaggaaaat gggatcgatg aaggggaaag gcgggcccgg agcgccggtg gactccgacg
     1081 tccgcgtggc ggtgtgcggt gcggggctgc gcgtctggtt cccctggccg gcacgcgcgt
     1141 gcagcgtcgt cgacgtgcgc gggctccccg ctcgggcgag cgtcgggaag ggcctgacca
     1201 tcgggctgga cgggctcccg gaaaacgtca tgggcctgct ggcgcgggtg gagacgcggg
     1261 ccgcggaaat cgatgtcgcc ggcgcggcgc tgcgggttca cgtgcccggc ggacacgcgg
     1321 aattccagat ggaaggcgag tccacggtcg cgctgcggct ttgcggccgg gaactcgtgg
     1381 gcatcgacgt ccgcttcctt ggaaggccgt gaccattcgt ccaccggcgg ttcggcctgc
     1441 cgtacgatcc ggcacgacgg ggcgccgaac ggggaggccg atggaccggc gggagaagaa
     1501 cggcaaggtc gcctgggaac tgacggcggg aatcgaggag gagcggcagc ggctgcggcg
     1561 cgagatccac gaccagatgg cgcccgccct cgcggcgttg cggctgcgct tcgacctggc
     1621 gcgggaggtc gtcgccgacg gcgccggcgg tccggacccg ctcgattcgc tggattcggt
     1681 ggacgaggcg atccgcaatt tgggtgaggc gctctcgggc ctcctcgccg ccatcgacgg
     1741 cgcgtttccc gccgagttgg cgaggctcgg cctcatcggc gcgctcgccg atctgggcgc
     1801 cttcctctcc ctggccgatc tgcgcatcga ggccgacgag aacgaactgc tggagatgtc
     1861 gccggcctgc gaggtggcgg cctacaagat agccgccgag gcgctcacca acgcgcgcaa
     1921 atactcctcc gaccgcagtg ccgtggtgcg aatcgaccgg caggacgggg acatcgtcct
     1981 ccaggtcagc agcgccatcg gcagcgagcg cgagcgccac ccctcctacg gcgtcggatt
     2041 ccgctccatg cgcgagcggg ccgccgccct cggcggcaaa tgcaacgtcc gcgcgaccga
     2101 cgagggaatg gtcgtccggg cccggatccc gtcgcggccg gcatgagcgt tcccgttctc
     2161 atcgtcgagg accacccgct ctatctcggc gcgctgcgcg cgctcttcga gcgcggcgac
     2221 ggattcgagg tggtcgcgga ggccgccacc ggcgcggccg cgctggaggc gctggagggc
     2281 ccgcgcggcg cgcgaccgcg cgtgatcacc ctcgacctcc acctcccgga cggctcgggc
     2341 accgcgctgg cgcagcgcgt cctggccgcc catccccagg tgcgggtgct ggtcatcagc
     2401 gcgctgaccg accgggcggc ggtgctgtcg gcgctgcgcg ccggggtgca cggctacctg
     2461 accaaggacg ccggcagcga ggagatccgg cacgcggtgc ggctggtcgc ggccggcggg
     2521 acggccttcg gcgagccgat cgccgtgctg ctgcgcgact ggctgaaggc cgacgccggc
     2581 gcgtccgcga ggacgcactt cccgacgctc accggacgcg agacgcaggt gctcgacctg
     2641 ctcgccgggg gccgggagta cgcgcagatc gccagggagc tggggatctc ggagaagacg
     2701 gtgcgcaacc acctctccaa cctgttcgtg aagctggagg tgaacaaccg ggcgcaggcg
     2761 atcctggtgg cgcgccgggc gggcatgggc acctcctgag cggccctccg gcggggccgc
     2821 ccgcgcgtcg gtcagcgctt tcgcgggtca cgccggcggc gtccagcgcg ggtcgcggcc
     2881 gagcagggcg acgatggtgt cgatcgcgga acggccggag tcctcgggga ggggcgggag
     2941 gtagacgccg cccggcccgt tgaggacgct gccgggggcg gtgttggcgc gggcgagcgg
     3001 cagcgcggcc cggagcagcc cggccggtgt gtcgagcgcg gtgcccagcg cgcgggccag
     3061 gtcccagcgg tggaccagca tgtcgacgac gtggacgccg agcacccatt cggtcggcgc
     3121 cgggccccgc catgggaggt ccacggtcgc ccccgtccgg tcgagggccg tgagcgagcg
     3181 cccggcgacg cgggcgaagt cgtcccgggg gtcctcgccc ggttcgagag gcggcagcac
     3241 cgtccgggcg agggcctcgt gccgctcgtt catgtggtgg agcagatccg ccaccgtcca
     3301 tccctcgcag ggggtcgggt ccgccatcgc ggcgtcgggg atcagcgcca cgtcgtgccc
     3361 gatcagggcg agcgccgtgg cgtccaggcg gaacaggtcg gtcatggggg ctcccggtaa
     3421 tagcgaacga tcgtacgcca aaactataac gtacggtcgt tcgctatgat gggcccgtgt
     3481 ctcctcgcca ctcgatcgcc gaagcggccc ggacgcgcga ccggatcgtg cgggccgcgg
     3541 tgcgggacgc ctcgcgcctc ggcctggagg ggctgacggt cggctcgctc gcgcgccggc
     3601 tcggcatgag caaggcgggc ctgatcggcc ccttcggatc ccgcgaggcg ctgctggcgg
     3661 cggcgctcga ccatgccgtg cagatcttcc aggcggcggt catcgccccg ctggaacagc
     3721 ggccccccgg cgcggaacgg ctggaactgc tcatcgaccg gtgggtggcc tacctggccg
     3781 actgcccgtt ccccggcggc tgcttcgtga cggccgcctc gtccgaactc gacggccgcc
     3841 cggggccgct gcgcgaccgg ctgcgcgagg gcgtggcctc ctggcgcgcg cggatcgccg
     3901 ccgaggtcgc cgcggcgcag gccgagctcc cgggcccgca ccgtcccgcg gaggaggtcg
     3961 ccgtgaccct ggtcggcctc tcgatggccc tcaaccagga ggtccagctg ctggaggacc
     4021 gcgaggccgc cccccgcgcc cgcgcggcca tgcggcatgc cgcggggctg cgtccctgag
     4081 cgccggagaa ccgcgaaacc cgcgagagta acgcgggccg gagtggtccg aaacccccca
     4141 atatttgggg atgggccggg ggagtcgtcc ggaacaggat gtgcgtcatt cggatccgca
     4201 tcatccgacc aagggaagcg aaatgaaacg gacgactcgc gtgttggggg cggcgctggc
     4261 gttgcttttc gtcggtgtcg gcaacggcac ggcgttcgcg cagccggcgc aacccgcaca
     4321 gcccgcacag cccgcacagc ccgcgaagac cacccggacc gcgaaggccg ccgacgcgaa
     4381 gatcctggcg gtacggcact tctacaacga ccggttccag cggtgcctgg acgccgacac
     4441 caacaccggg ggcaacggcg gcatcgtcca gctgtgggac tgcggcccct ggaaccagca
     4501 ggactgggag gtctggccgg acggccacat ctacaactcg cgcttccaga agtgcctgga
     4561 cgccgacacc aacaccgggg gcaacggcgg caaggtccag ctctggggct gcggcccctg
     4621 gaaccagcaa tggtgggaca tctggcccga cggccacctc tacaacaggc gtttccagcg
     4681 gtgcctggac gccgacacca acaccggcgg caacggcggc atcgtccagt tgtgggactg
     4741 cggtccctgg aaccagcagt ggtggcgata agcggggtcg cgaggcaacc gggaactatc
     4801 cgccccggat gttcccccgc ccggcgcggt ggggcacagt acctctcgac actgccagtc
     4861 acgcagagtt agggagcgag tgcaatgggc tgtgaggtat accgctgcgg tcccaacatc
     4921 gccgtcggcg agctgaccgc cgaggaggcg gaacgcctcc agcggatgga gcggcctcag
     4981 gccgaggacg agtcggccag ttgaccgcgc aggacaggta ccaacggagc ggtcccctcc
     5041 cccgggaggg ggccctctgg tgcgcggacc acttccgcgg cgcccgcggt ttccgggccg
     5101 ccgcgccgca cgccggagaa cgcgcgttcc cgctgccgcg cgccagggcc ctgatcgagg
     5161 cggcgctgga cgaggcggcg ctgcgccccc ggctgaccga tcttcgcggc gcctggcgct
     5221 gccacctctc ccatcccgac ggcacccccg tccgcgacgg ggtgggcgcg gggaagggag
     5281 acccgcagtc cgcgcaggtc ggggccctgt acgaggcgct ggagcaccac cgcggcgccg
     5341 ccctccccgc gcgcggcgaa ccggtcgagc tgctcaccgc cgcccaactc gccggtaccg
     5401 acctggcgcg ggacccggcc ctggagccgc tcgccgcgtc cggcgggccg ggggcggcgg
     5461 cgtgccggcg ctacaccgcg ctggaccact ccggccgcac gctcccggtg ccggtgttcc
     5521 tcaccaaccc cgcctacctg gaatgcccgg ccgaccgccg ccacgagctg ggggacgtcc
     5581 tcgactacgc cccgctgagc cggttcagca ccaacagcgg caccgcgatc ggcgcgaccc
     5641 gcgacgaggc cctggtgcac gccctggccg aggcggtgga acgtgacgcc ttctccttgc
     5701 tgctggccgg aacgttcctg ctcgcccgcc cgtcgccgct gcggctggtg cgtccggcga
     5761 gcctgcccga ccacctccag gagctctacc tgagcgccgg atggcacgcc ggaggctcgg
     5821 tcgcgttgat cgacatgacc agcgacctgg gcgttccggc gttctgcgcg cacacctccc
     5881 gcgacgggag ggcggcgcag gggcagggcg cgtcgctgtc ggccgagcac gcggtgcggc
     5941 gggcgctgaa cgagctgatc caggtcgtcg cgatccggcg cggcgagcgg aacccgccgg
     6001 cggacctccg cgcggtggaa agccacccgc gcctgctgga ggcggcacgc gccgacttcg
     6061 acccgcatct ggccggtgcc cgcccgcgcg acttcctccc gaccggcgcg cccgccgcgc
     6121 cccgcgagca cctccagcgc ctcacggcca cgctcaccgg ctacgggtac cgcccctacg
     6181 gccgggtcct gcgcaccgag cccaacggcg tgaccacggt gtcggtgttc gtgcccggtc
     6241 tggaacgctt cttcgcggtc accgccggtg cctgcgtcgt ccccggcccg cgcggccgcg
     6301 ccctcggccc cggatgacct cccacgacgt ggtcgtcgtc ggcggcgggc ccgccgggct
     6361 cagcgccgcg ctggtgctgg cccgcgcgtg ccgcgccgtg gccgtcgtcg acgccgggcg
     6421 gccccgcaac gccgcgtccg cccacgtcca cggcgtgctg gggcttcccg acctgtcgcc
     6481 gcaggtcctt ctggaggtgg gccgcgcgca ggtcctcgcc tacggcgtcg acgtgctgga
     6541 ggggacggcc cgcacggcgc ggccgcgtcc cgagggcggg ttcgccgtca ccctgtccga
     6601 cggcagggtc ctcgccgggg cgcatctgct gctcgccacc ggcaccgtcg acgagctgcc
     6661 cgacattccc ggcctggccc cgcgctgggg gcgcgacgcc gtcgcctgcc cgcactgcca
     6721 cggctgggaa ctgcgcggcc gcgcgatcgc gatcctgggc ggtggccgcg cggacgcccg
     6781 ccgggccctg atgtggcggc agtggacccc gaacctcacc ctgatcaccg cccccgacca
     6841 cccgctcgcc ccgtccgacc tcgaacggct gggcgcgcgg ggcatcaccg tcctcgacgg
     6901 ccccgtcacc gaggtgatcg ccgccgccga ccggctctgc ggggtgcggc tgcgcgacgg
     6961 gacgtccgtc ccgatccgcg ccctggtggt ggccccgtgc ttccgggccc gcgccgacct
     7021 gctggacggc ctcggcctgc gccccaccga gctgacggtc ggcggccggg tcgtcggcac
     7081 cgtcatcgcg accgacggcg ccggacgcac cgccgtgccg ggggtctggg ccgccgggga
     7141 cggcgcgtcc ccgctggagc gggtcgtcgc ggtcaccgcc gacggcgcgg cggtcgcgtc
     7201 cgcgatcaac gcggccctca ccgacgggtc gcacccggag cactccgctg gggagccgtg
     7261 acctccgcat gggccgacat gcgttttctt aatgatctgc cgtgcatatt cccatggaac
     7321 aggtaatggg ccgctcccgc cccgagttcg tggtcccatg agttcggaga aggagatcac
     7381 cggggaattc gcgcagaaag aatcgacgat atgagacatg ggttgattcg cgtggtcgcg
     7441 tcgatcgggg ccgtgctggc cgtcgcgtcg accgggcacg ccgtggccgc gcccgcgggc
     7501 ccggggccgg tcctggtgga cgagccgttc acccgcgcca ccgccgactc gcgcttcatc
     7561 ggatacggcg ccgcctgcct gacgggcgcc gcgcggggga acgcgccgca ggagggcgcg
     7621 aaccacgcgc tctcgggctg ccgccgggat ccggccggtc cggtgccgcc gggcggcgcc
     7681 gcgccgcgcg gatacctcca gctcaccgac gcccaccccg aggtgaccgg cgccgtgatg
     7741 ttcgactcgc ccgtcccggc gcgggacggg ctggaggtga ccttcgagca gtggcagtac
     7801 ggcaacacca cccgggcgcc cgccgacggc atctcgttct tcctcaccga cggcgcgcgg
     7861 tcgctgcaca ctccgggcgc gttcggcgga agcctcggat atgcgcagaa actgccgggc
     7921 ggcaggtcgg acgcgcactt cgtccccggc gtcagcggcg gatacctcgg cctcggattc
     7981 gacgtgctgg gggagtactt cggcgacggg gaaaggcgcg ggaacggctg cgagcggcgt
     8041 tccccggccg ggacggagtt ccgcgcgccc gctcccgggc ccaacatcgt gaccgcgcgc
     8101 ggccccggga acggcatcga cggctactgc ctgctcgacg ccacgacgag caacaggtcg
     8161 tccaccggcc cctggacgtc gagcctgccc ggacgcctgc acggcgacct gaggacgatg
     8221 cccgccaggg tcaccgcgaa gaaggccgag ggcctgctgg agccggtgaa gcgggtcgtc
     8281 cgcgtcgtcg tgacgcccgc gccgaacccg gtggtcacgg tcgacatcga cttccaggac
     8341 gacgagggct tccagcgggt cctgcggttc ccggccccgc agccggtccc ggcggcctac
     8401 cggttcgggt tcgccgcgtc caccgggccg ctcaccgatg tgcagctcat ccggaaggtc
     8461 gtcctgcgcg cccagcgccc ggccggagac gaggagccgt ccgagtccga ggacgaatga
     8521 gcgggccggg ccggccgcgc acgcgcggcc ggcccggtcc gccggtgggg ccgcggctca
     8581 gaggcggagg tcgcccaggt cgccgcggta gtggccgggg tcacggcggt gggcccggag
     8641 cgcggtgacc ttcgtcccga gcatgtcgcg cgccctcggc aggcgctggg ccgggaactc
     8701 ggtcggggtg tcgtggtcgg ggacgagccg cggggtggtg ccccggtcgg cgatccgcgc
     8761 cgaggtgatc gtgagttcgt agtgggccca caccatggcg gcgccgggca gcctgcgggc
     8821 cgtggcatgg acgaggtaga cgtcggacag ggcggtgacg atcagcagcg cgggcgcgtc
     8881 gcggtcgagg ttgaccgcgg tggggtcggt cggccagacc gacacccgca ggtgccggtc
     8941 gccgacgacg cgcagttcgc cccagtccgg gacgcagacc tggggcagcc cgtcggcgcc
     9001 acaggtgatc atgacgacgg gacgttgccg ggacggggag ttggtgatcg ctttgacggc
     9061 ggctgggagc acctcgggtg agaacgacat cggtgtgtcc gcccttacgt cgaaggtccg
     9121 ccggcccggc gtgcggccga gccggtcttg ctatgaatcc ttcccgatat tgttcgatca
     9181 tacattcgag agtgctaacg atcacacggc tttttccgag tggcgccacg cgtggggcgc
     9241 acgtttaccc tggtgctgca tggcccaaac cacagccggg cccactaccg gggccgttgt
     9301 gaaacggcgc cgatcgtggg cccggctgtt cgattgaggg tcagccgacc gcccgcttca
     9361 caaggtcgct gatcacggcc tcgttcgcgg cgtccagggc cgtcagcgcg taggtcgtcg
     9421 gccacagcgt gccgtcgtcg agcgcggcca cgtcgctgaa gccgagcgtc gcgtaccgcg
     9481 acttgaactt ctgcgcgctc tggaagaagc acagcacctt gccgtccttg gcgtaggcgg
     9541 gcatcccgta ccaggtccgc gggacgaggc ccggcgcgac cgaggcgatg atctcgtgca
     9601 gccgctcggc gagcacccgg tcggcctccg gcatctcggc gatcttctcg cggacgtcgc
     9661 gctccccctc ctccctggcc ttggcctcgg acgcgctgcg ccgcgcggcc ttcttcagct
     9721 ccttggcgtg ctccttcatc gcggcccgct cgtcctcgga gaacccgtcg aagttcttgc
     9781 cggtcgcggc gtcgcccttg gcggccttcg gtgtctcgct catgatggga atccccttct
     9841 ctcggtttct cgctggcggg ccctcggcgg agggccgtcc ttctggtctg gttatcccca
     9901 ggccccggcg atctgccggg tggtggcgtt gagccggttg aacaggttgg tgagcgcgat
     9961 catgaggacg atcgcggcga gctgcttctc gtcgtagtag gtggcggcgg cgtcccaggt
    10021 ctcgtcgtcc accgcgtccg ggcggtcggc cagccgcgtc gcggcctcgg ccagcgccag
    10081 cgccgcccgc tcctcgtcgg tgaagtaggg ggcctcgcgc caggcggcga ccagggccag
    10141 cttgtcgtcg cccacgccgg ccttgcgggc cgacttgacg ccgctgtcga cgcacgggct
    10201 gcacccgttg atctggctgg cccgcaggtg caccagctcc agcgtctgct ggtccacccc
    10261 gccggagtgc gcggccttga acaggtcctg gatcggccgc atcgcaccgg tcaggacggc
    10321 ggcggggttc tgcatccggg actgcatctc ggtgttctcc ttcgtgtgat cggccgcccc
    10381 gttctctcgg ggcatggctc catcacagcg ggacgggccg cgcatgccga tggccgtggg
    10441 acaccgtggg acagtcgaaa cacccctgag cagggctaat ctatccagaa gagttgtacc
    10501 ggcatgggac agggaggcac cgggcggatg accgtggaac accggacacc gcaggccggc
    10561 ccgcgcgcgg acccccggga cgagctggcc gcccggctgc gcctgctcca ggaggtgtcc
    10621 gagcgcggcg tccgcgccct cgcgcgcgac gccggcctca gctcgtcgtc gctgtcgcgc
    10681 tacctcggcg gccagaccgt gccgccctgg cccgccgtga tcgcgctgtg ccgcctcgtc
    10741 cagcgcgacc cgcgcccgct gcgcccgctc tgggagcgcg cggcgaaccc gctgcccgcg
    10801 ccgccgaaga cgagccgcca ggtccagccg ccgtcgccgc ccgccggggc gccgcgcccg
    10861 ccccgcaacg acctgccgcg cgacctgccc gacttcaccg ggcgcgaggc cgagctggcc
    10921 gccgtcctcg ccgccgtgga ggccgaccgg gtcgtcgcgc tcgacggcat ggcgggcgtc
    10981 ggcaagacct gcctcgcggt gcgcgccgcg caccggctcg ccgccgccta ccccgacgcc
    11041 cagctctacg tcgacctgca cgggttcacc gccgggcgcg agccgctcga ccccgacgcc
    11101 gcgctgcgga cgctgctcgc cgccctcgac gtcccgtccg agaagatccc gcaggagggc
    11161 ggcatcgagc cgctggccgc gtgctggcgc tcggagctgg cgcaccggcg ggccgtcgtg
    11221 gtgctcgaca acgccgcggg cgccgagcag gtccggccgc tgctgccggg cgccgggccg
    11281 tcggtcgcgc tgatcaccag ccgcaaccgc ctgctgg
//
