LOCUS       NZ_WOFH01000005        11905 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold5, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000005
VERSION     NZ_WOFH01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 653447)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 526280
            Orig. end                         :: 538185
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11905
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11905
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11905
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6905](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6905)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    883..898
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.88"
                     /tool="antismash"
     gene            992..1696
                     /locus_tag="GNZ18_RS17165"
                     /old_locus_tag="GNZ18_17160"
     CDS             992..1696
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="GNZ18_RS17165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17160"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_308165793.1"
                     /transl_table=11
                     /translation="MTSILLCSANADCRDRVAGVLGRTLGGQVRLVVPDGRSVAAALVA
                     AATDLLVVDLSPWRAGHGDLVRAVCREGRVLRVPVVLLVDHTARFGEAAGRPELLAALW
                     AGVASIADRRGPVAEVTAAARTAAAGGRYLAPAFACCLLDDPVDGPALLDELFEADPHR
                     PGVLTPREREVLALLASRPSNAWIAERLALSEKTVKYHVSNVLGKLGLRDRGEAAALAA
                     SMSPDHCGTLAS"
     gene            complement(1701..3173)
                     /locus_tag="GNZ18_RS41230"
     CDS             complement(1701..3173)
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908627.1"
                     /locus_tag="GNZ18_RS41230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protein kinase"
                     /protein_id="WP_312874578.1"
                     /sec_met_domain="Pkinase (E-value: 1.6e-38, bitscore:
                     127.7, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDLRPEDPRTVGRYTLTARLGAGGMGQVYLGSSPGGRPVAVKLV
                     HAGFAADAEFRRRFKREVEAARRVGGFHTAPVVDADPDADTPWLVTAYVPGPSLASAVA
                     EHGPFPPASARVLGAGLAEALEAVHGAGVVHRDLKPSNVLIAADGPRVIDFGIARAVDA
                     SGITTRAGTPGFMAPELITDGASTPACDVFALGAVLAFAVGVRPFGEGPSEALTYRVVH
                     REPDLGGVPEPLGPLVAACLAKDPAARPAPSDLLGALTPGGDAGAWLPPPVQEMVTRFP
                     TAHTPTPRPAPTRQLPSPQAAPPARQAVRIAGRITPAAIALAASVLYSLIWVAIFLLGA
                     GLALGGTSSDADSVATLIGVVVAIVGAVNCVRAIPRAVWQAMALARPVTFEIGPGGLSL
                     RHGGRRVHYGWHEIGRVVLRRIDDKKKRALCVSLNPGSAQPDYLSPRSPFCYTEADTGW
                     VVIAPLIRLRTSRREIEGIVVRYAGPRWGGHA"
     gene            complement(3260..3631)
                     /locus_tag="GNZ18_RS17175"
                     /old_locus_tag="GNZ18_17170"
     CDS             complement(3260..3631)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887062.1"
                     /locus_tag="GNZ18_RS17175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17170"
                     /product="DUF4267 domain-containing protein"
                     /protein_id="WP_156217435.1"
                     /transl_table=11
                     /translation="MLAPIAYGLAVLLSLFVLFIGARFLVQPLPAAAGYGVPAKPDGDP
                     AYLSVKGVRDAAFGLLGLALVAFSGADAAAWYMLVVALVPLGDTVIVLRNGGTRAAAFG
                     IHFATALVVLLSAALLFAV"
     misc_feature    3676..3696
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.68"
                     /tool="antismash"
     gene            3721..4290
                     /locus_tag="GNZ18_RS17180"
                     /old_locus_tag="GNZ18_17175"
     CDS             3721..4290
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887080.1"
                     /locus_tag="GNZ18_RS17180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17175"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_156217436.1"
                     /transl_table=11
                     /translation="MSIQARRERERAERERAIVTAARELAEAEGWDAVTTRRLAAEIEY
                     SQPILYSHFSGKDAIMAAVALEGLADLAAVLRAARPGGIAAVAAAYAEFGERRPALYDA
                     MLTLAVDLEFASPESPAPLREGFGELRETMRPYAGDDDLEMLTETFWSGLHGLHMLMRH
                     GRLRRADHERRLALLIGRFSSPVRSR"
     misc_feature    3925..3927
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4277..4984)
                     /locus_tag="GNZ18_RS17185"
                     /old_locus_tag="GNZ18_17180"
     CDS             complement(4277..4984)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.4"
                     /locus_tag="GNZ18_RS17185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17180"
                     /product="DUF5825 family protein"
                     /protein_id="WP_156217437.1"
                     /transl_table=11
                     /translation="MIRDLPPVDLTSAAGVAAARGRPDVRVLEVSLADLASGTAVPRGK
                     QEPLTWLRVRPTGAELDGGPWPGDDALGALPAAGVVGLTLEAPALPRAPWLIAFLVRAT
                     SFQLPLEWGGPVADLPCGLLFHLAPPAFGDEVAGKWRAAHRYGQCYWRRGPGFAAVQDL
                     REDPGAHFVIHEPGLLALFHRLADPVEVADLGADDRAHLRDLLDARLAVELGGVAVGLP
                     YRLRRWPAPVIDF"
     gene            complement(5001..6905)
                     /locus_tag="GNZ18_RS17190"
                     /old_locus_tag="GNZ18_17185"
     CDS             complement(5001..6905)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="GNZ18_RS17190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17185"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_156217438.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.6e-157, bitscore:
                     519.9, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.5e-11, bitscore: 40.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVTLAVMPWHLMTAPNGAAGVLAAALAPDHEVTTYYGSISFAEH
                     LLGEAETAEEGTARARLLPEAYMYVAEEGFTGGIGEWLFTGALYGHRSFPDEASAACAA
                     IGFDPDVAEQIFELAPAFADAAAQEIIAGRPEVVGFTTTFSQTIPALAVARRLRALDPD
                     ITIVFGGSNCDDTMGAALHRCFPEIDFVVRREGEAPLRSLLAALAEGDQGRPASIPGLC
                     WRDADGTSRANPEGGALPSGAQFPPMDQSAYFERLEESPVADYIQPALMMESARGCWWG
                     ERHHCTFCGLSDLILPFRSKAPDRVVEELLAGIERHQVLDVLTTDNIVDKNYYETVFPT
                     LEKSDLDLRVMYEIKSNITPEQARALRGAGVIQVQPGIENLHSLPLKLMRKGVTGANNV
                     ATLRELQEQSLTVLWNYLVGFPGETDGCYQEVITQIPLLYHLQPPLGAHRIALERFNPY
                     FDHPDLGFAERRPRSWYRGVFPDLGEPDLNDVAYLFETPDQGVGGATLTGLKAAFDAWR
                     EAYATSALTHRAVAGRLHLYDRRAGRRAADHVLDEPAEVAAYAALGRGRSPEGLARHLS
                     AGGWPVDAAWASAFIDDLTALGLVFRESGRAVTLSTGHDATAVRGTAIPTPAMVGAGT"
     gene            7203..8447
                     /locus_tag="GNZ18_RS17195"
                     /old_locus_tag="GNZ18_17190"
     CDS             7203..8447
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS17195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_17190"
                     /product="hypothetical protein"
                     /protein_id="WP_156217439.1"
                     /transl_table=11
                     /translation="MTGPNLGTGSANSYRALFAIPGTARLYFGYLLARMPVGVIGLSLL
                     LLGQDTGGTARSGLLVGAFSAALMIATPLLGRALDTFPPGRVLSLAATAQCAALALLAI
                     CWSTSEPETGLLLTGALLAGASLPPAGPALRVALPVFVPDDLLTTGFTAEAVILETVYI
                     IGPLLVGGLGAVAGWLPLAAAGGCTAAGVAVMLSSGRLAGACVDTPPARALWTLGPLAD
                     GGVRRLLLGAFLFLPALAVVEVGVAAVAGERDRPELLGVVMAFSAVASLAGGLLFGLRR
                     WPGSLASHVAVLAGWFAAASVLGALAPGPWPAGAAVVLGSLVVAPALALLGRIGGVVAP
                     EGRAAETYTWLSTMNNCGQAATLPLAGLLAERSGPEGAFLLAATLAGTAALFILNNRRH
                     LAPTTTSRPRETVRT"
     gene            complement(9595..10971)
                     /gene="gdhA"
                     /locus_tag="GNZ18_RS17200"
                     /old_locus_tag="GNZ18_17195"
     CDS             complement(9595..10971)
                     /EC_number="1.4.1.4"
                     /GO_function="GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_process="GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gdhA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593591.1"
                     /locus_tag="GNZ18_RS17200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_17195"
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="WP_156217440.1"
                     /transl_table=11
                     /translation="MPLSPRLQAPPETLALLEESYERVLRRDPGEPEFHQAVHEVLESL
                     PPVLAARPGLAAAKMVERVIEPERQIVFRVPWQDDAGEIHVNRGFRVEFNSALGPYKGG
                     LRFHPTVNLGVVKFLGFEQIFKNALTGLGIGGGKGGSDFDPHGRSDAEVMRFCQSFMTE
                     LQRHIGDHTDVPAGDIGVGGREIGYLFGQYRRVTNRWEAGVLTGKGLPWGGSAGRTEAT
                     GYGNVMFTAEMLRRRGEDLDGQQVVVSGSGNVAIYTIEKAQALGANVVTISDSGGYVVD
                     EKGIDLDLLKKVKEVDRGRVSDYAEQRGDSAQYVAGGSVWDVPADIALPSATQNELDES
                     AAAVLVRNGVKAVSEGANMPATPGAVHVFQGAGVAFGPGKAANAGGVAVSALEMQQNAG
                     RESWTFERVESELSGIMTSIHDTCFETAERYGAPGDYVVGANIAGFERVADAMLAQGLI
                     "
     misc_feature    11067..11084
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    11074..11089
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.19"
                     /tool="antismash"
     misc_feature    11891..11905
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
ORIGIN
        1 tcgagtactg gaaggggaac ggcgccgacg cggtcgactt cgcgcggcgg gccgtggaca
       61 ccgccggcga ccagggcgag tggtggggca agcaggccga gctggcgctc ggcctggcgc
      121 tggtggagac ggggcaggcc gaggccgggg cccggtccct gctggaggcg gccaacgggt
      181 tcgcccgtcc cgggtccgac ctcgtcacgc tggtccaggt ctccgagcgg ctcgccgagc
      241 tggaggccgc gcgggggcgg cacgccgcgt ccgccgagtg ggcggccgag gccgacaagt
      301 actcccacgc gagcctgccc gtctcgaccg gcctcgccgc gctcgcccgc gcgcactccc
      361 tgctgagcac cgacccgggc gccgccgccg agcacgcccg gggcgccgcc gccgtgctgg
      421 cgggcaacga gtacccgctc gacgccgggc gggcgctgct caccgccggg accgccgccg
      481 cgcgcgcgtc ccgccgcgac accgccaagg ccgacctgcg gcgcgcggcg gaggtgttcg
      541 gcggctgcgg cgcggcctcg ttcctgcggc gggcggtgcg ggagcagcgg cggctcggga
      601 tctacgtccc ggtgcgcggc cgctcgtccg gcccgcacga gctgtcgcgg cgcgagctgg
      661 aggtggccga gctggtctcg gccgggcgga ccaaccagca gatcgccgag gcgctgttca
      721 tcagcgcccg caccgtcgag agccacctcg cgcacatctt cgccaagctg ggcgtctcgt
      781 cccgggcggg cgtcgtcagc gcgctcaggt ccccctcctg agccgcggtc gcggcgctcc
      841 tggggtgcgg ccgccgggca ctacgtaacc ccgggaatcg gcaatatcag tgattctccg
      901 gatgtgcgtc cgtaacccca tcggcgaggc tcgcggcaga ggtgatcggg tgatcgggtc
      961 ccgttcgccg aggcgccggg aggtggggga cgtgacgtcg atactgctgt gctcagccaa
     1021 cgccgactgc cgcgaccggg tggcgggcgt cctcggccgg acgctcgggg gccaggtcag
     1081 gctcgtggtg ccggacggga gatcggtggc cgccgcgctc gtcgccgccg cgaccgacct
     1141 gctcgtggtc gacctcagcc cgtggcgggc cgggcacggg gacctcgtcc gagcggtgtg
     1201 ccgggagggg cgcgtgctcc gcgtcccggt cgtgctgctg gtggaccaca ccgcgcggtt
     1261 cggcgaggcc gccgggcgcc ccgaactgct ggccgcgctg tgggccggtg tcgcgagcat
     1321 cgccgaccgg cgggggccgg tcgccgaggt gaccgccgcc gcccggacgg ccgccgccgg
     1381 cggccgctac ctcgcccccg cgttcgcctg ctgtctgctg gacgaccccg tcgacgggcc
     1441 cgcgctgctg gacgagctgt tcgaggccga cccgcaccgt cccggtgtgc tgacgccgcg
     1501 cgagcgggag gtgctggcgc tgctggcgtc ccgtccgtcc aacgcgtgga tcgccgagcg
     1561 gctcgcgctg agcgagaaga ccgtgaagta ccacgtgtcg aacgtcctcg ggaagctcgg
     1621 gctgcgcgac cggggcgagg ccgccgcgct ggccgcgagc atgtcgcccg accactgcgg
     1681 gacgctggcg tcctagcgcg tcaggcgtgg ccgccccacc gggggcccgc gtaccggacg
     1741 acgatcccct cgatctcccg ccgcgaggtc cgcagccgga tcagcggggc gatgacgacc
     1801 cagccggtgt ccgcctcggt gtagcagaac gggctccgcg ggctcaggta gtcgggctgc
     1861 gcgctcccag ggttcaggga tacgcacaac gcccgcttct tcttgtcgtc gatgcggcgg
     1921 agcacgaccc gtccgatctc gtgccacccg tagtggacgc ggcgtccgcc gtgccgcagg
     1981 ctgagcccgc cggggccgat ctcgaacgtc acgggccgcg ccagcgccat ggcctgccag
     2041 accgcgcgcg gaatcgccct gacgcagttc accgcgccga ctatggccac gacgacgccg
     2101 atgagcgtgg cgacgctgtc ggcgtcggag gaggtgccgc cgagggcgag cccggcgccg
     2161 agcagaaaga tcgcgaccca gatcagcgag tagaggacgg acgcggcgag cgcgatcgcg
     2221 gcgggcgtga tccgccccgc gatccgcacg gcctgccgcg cgggtggggc ggcctgcggc
     2281 gacgggagtt ggcgcgtcgg tgccggcctg ggagtgggcg tgtgcgcggt ggggaagcgg
     2341 gtgaccatct cctggacggg cggcggcagc caggcgcccg cgtccccgcc cggggtcagg
     2401 gcgccgagca ggtcggacgg cgccggacgg gccgccgggt ccttggcgag gcaggccgcg
     2461 accagggggc cgagcggctc cggcacgccg ccgagatcgg gctcccggtg cacgacccgg
     2521 taggtcagcg cctccgaggg tccctcgccg aacggccgca ccccgaccgc gaaggcgagc
     2581 acggcgccga gcgcgaacac gtcgcaggcc ggcgtgctcg cgccgtccgt gatcagctcg
     2641 ggggccatga agccgggcgt cccggcgcgg gtcgtgatcc cggacgcgtc gaccgcgcgg
     2701 gcgatgccga agtcgatgac gcgcggcccg tcggcggcga tcagcacgtt ggacggcttg
     2761 aggtcgcggt gcacgacccc cgccccgtgg acggcctcca gcgcctcggc cagcccggcg
     2821 ccgagcaccc gggccgaggc cggggggaac ggtccgtgct cggcgaccgc cgaggccagc
     2881 gagggcccgg ggacgtaggc ggtgaccagc cagggcgtgt cggcgtcggg gtcggcgtcc
     2941 acgaccggcg cggtgtggaa cccgccgacc cgccgggccg cctccacctc ccgcttgaac
     3001 cggcgccgga actcggcgtc ggcggcgaaa ccggcgtgga cgagcttgac ggcgacgggc
     3061 cgtccgccgg gcgatgaccc gaggtagacc tggcccatcc cgcccgcgcc gaggcgcgcc
     3121 gtcagggtgt accggccgac ggtacggggg tcctcgggtc gcagatcgtc cacagcccct
     3181 cctccggtcg tgacggcccc cgcccaagat tcaagcggtg cggggaggtt tcggaaagcc
     3241 ccgggacggc gtccgtcgct cagaccgcga acagcagggc ggcgctgagc aggacgacca
     3301 gcgcggtcgc gaagtggatg ccgaacgcgg cggccctcgt cccgccgttg cgcagcacga
     3361 tcacggtgtc gccgagcggg acgagtgcga cgacgagcat gtaccaggcg gcggcgtcgg
     3421 ctccggagaa ggcgaccagg gccaggccga ggagcccgaa ggcggcgtcg cggaccccct
     3481 tgacgctcag gtaggcgggg tcgccgtccg gcttggcggg gacgccgtag ccggccgccg
     3541 ccggaagcgg ctggacgagg aaccgcgcgc cgatgaacag gacgaacagg ctgagcagga
     3601 cggccaggcc gtaggcgatc ggtgcgagca tgacgactcc cgttgagtta gcagtgctag
     3661 gaacgtgatc gacgttagca gagcattggc aagatgacta gcgatgctag gatcatggcc
     3721 atgtcgattc aggcgcgccg ggagcgcgag cgggcggaac gggaacgggc gatcgtcacg
     3781 gccgcgcggg agctggccga ggccgagggc tgggacgcgg tgaccacgcg gcggctcgcg
     3841 gcggagatcg agtacagcca gcccatcctc tacagccatt tctcgggcaa ggacgcgatc
     3901 atggccgcgg tcgcgctgga ggggttagcc gacctcgccg ccgtactgcg cgcggcccgt
     3961 ccgggcggca tcgcggcggt cgcggccgcc tacgccgagt tcggcgagcg gcggcccgcg
     4021 ctgtacgacg cgatgctcac gctcgccgtc gacctggagt tcgccagccc ggagtcaccg
     4081 gcgcccttgc gggaggggtt cggcgaactg cgcgagacca tgcgccccta cgcgggcgac
     4141 gacgacctgg agatgctcac cgagacgttc tggagcggcc tgcacggcct gcacatgctg
     4201 atgcgccacg gccgcctgcg ccgcgccgac cacgagcggc ggctcgcgct cctgatcggc
     4261 cggttctcct ccccggtcag aagtcgatga cgggcgcggg ccagcggcgc agccggtagg
     4321 gcagcccgac cgcgacgccg ccgagctcga cggcgagccg cgcgtccagc aggtcgcgca
     4381 ggtgggcgcg gtcgtccgcg ccgaggtccg cgacctcgac cgggtcggcg aggcggtgga
     4441 agagcgcgag caggcccggc tcgtggatga cgaagtgggc gcccgggtcc tcgcgcaggt
     4501 cctggacggc ggcgaacccg ggcccgcgcc gccagtagca ctggccgtag cggtgcgccg
     4561 cccgccactt gcccgcgacc tcgtcgccga acgccggggg cgccaggtgg aacagcagcc
     4621 cgcacggcag gtccgcgacc gggccgcccc actccagcgg gagctggaac gaggtcgcgc
     4681 ggacgaggaa ggcgatcagc cagggcgcgc gcggcagcgc cggggcctcc agcgtcaggc
     4741 cgacgacccc ggcggcgggc agcgcgccga gcgcgtcgtc gccgggccac gggccgccgt
     4801 cgagttcggc cccggtcggg cggacgcgca gccacgtcag cggctcctgc ttcccgcgcg
     4861 ggaccgccgt gccggacgcc aggtcggcca gggacacctc caggacgcgg acgtccgggc
     4921 gcccccgtgc ggcggcgacc ccggcggcgc tggtcaggtc gaccggcggc aggtcgcgga
     4981 tcatcgtgct ccccgaagga tcatgtgccc gcaccgacca tggcgggcgt ggggatggcc
     5041 gtgccgcgca ccgccgtcgc gtcgtgcccg gtcgacagcg tcaccgcgcg ccccgactcg
     5101 cggaagacga ggccgagcgc ggtgagatcg tcgatgaacg ccgaggccca ggcggcgtcg
     5161 accggccacc cgcccgccga caggtgccgc gcgaggccct ccggcgagcg gccccgcccg
     5221 agcgcggcgt aggcggcgac ctcggccggt tcgtccagga cgtggtcggc ggcgcgccgc
     5281 ccggcccgcc ggtcgtacag gtgcagccgc ccggcgacgg cccggtgggt gagcgccgag
     5341 gtcgcgtacg cctcgcgcca ggcgtcgaac gccgccttca acccggtcag tgtggcgccg
     5401 ccgacgccct ggtcgggcgt ctcgaagagg taggcgacgt cgttcaggtc cggttcgccc
     5461 aggtcgggga acacgccccg gtaccaggat cgcggacggc gctcggcgaa tcccaggtca
     5521 gggtggtcga agtacgggtt gaaacgttcg agcgcgatgc ggtgcgcgcc gagcggcggc
     5581 tgaagatggt acagaagggg gatctgggtg atcacctcct gatagcaccc atcggtctcg
     5641 ccggggaagc cgacgaggta gttccacagc acggtgaggc tctgctcctg gagttcgcga
     5701 agcgtcgcga cgttgttcgc gccggtgacg cccttgcgca tgagcttcag cggcagcgaa
     5761 tggaggttct cgattcccgg ctgcacctgg atgacccccg cgccgcgcaa cgcgcgcgcc
     5821 tgctccggtg tgatgttcga cttgatctcg tacatcaccc gcagatcgag atcgcttttc
     5881 tccagtgtgg ggaaaaccgt ttcgtagtag ttcttgtcaa cgatgttgtc ggtggtgaga
     5941 acatcgagca cctgatgcct ctcgatgccc gcgagcaact cctcgacgac ccggtccggc
     6001 gccttcgacc ggaacggcag aatgagatcg gagagcccgc agaaagtgca gtggtgccgc
     6061 tcgccccacc agcatccgcg cgccgactcc atcatcagcg cgggctggat gtagtcggcg
     6121 accggcgatt cctccagtcg ctcgaaatag gcgctctggt ccatcggcgg gaactgcgcg
     6181 ccgctcggca gcgcgccgcc ctcggggttc gcgcgggacg tcccgtccgc gtcgcgccag
     6241 cacaggccgg gaatcgaggc cggccgccct tggtcgccct cggccagcgc ggccagcagc
     6301 gagcgcagcg gcgcctcgcc ctcccgccgc accacaaagt cgatctccgg gaagcaccgg
     6361 tgcagcgcgg cgcccatggt gtcgtcgcag ttggagccgc cgaagacgat cgtgatgtcc
     6421 ggatcgagcg cccgcagccg ccgtgcgacc gcgagtgccg gaatggtctg ggagaacgtc
     6481 gtggtgaacc ccacgacctc gggacggccc gcgatgatct cttgcgccgc ggcgtcggcg
     6541 aacgccgggg cgagctcgaa gatctgctcc gcgacgtccg ggtcgaagcc gatcgcggcg
     6601 caggcggcgg acgcctcgtc cgggaaactc cggtgcccgt agagcgcgcc ggtgaaaagc
     6661 cattcaccta ttccgccggt gaatccctct tcggcgacgt acatgtacgc ctcgggcagc
     6721 aatcgcgctc gcgcggtgcc ttcctcggcc gtttccgcct cgccgagcag gtgctcggcg
     6781 aaggaaatgc tgccgtagta cgtggtgacc tcgtggtccg gcgcgagggc cgcggccagg
     6841 acgccggccg cgccgttcgg cgcggtcatg aggtgccagg gcatgaccgc gagggtcacc
     6901 cgcacgtgtc gacctccggt ggtggatggg aaacgagtgc cggacggaaa cgtggcgcga
     6961 acccgaaaag gggcccggcc acgctcggga gccgtcagaa ctcgtggcgc tggctggccg
     7021 tctgctgctt acggattacc tttcgcttca cgggtcctcc cttctgggct ggaagtttca
     7081 cgcacctttt ctacccgccg cgacgagggc caccgcaccg gggtggccga tgtcggccaa
     7141 gatcaggcac ttttccgcca agccgtgtgc ggaccgtgct tacctgggtg ctggaggtga
     7201 aaatgacggg gccgaacctg gggacgggaa gcgccaactc ctatcgtgcc ctgttcgcca
     7261 ttccgggcac cgcgcgcctg tatttcggat atctgctcgc ccggatgccg gtcggcgtca
     7321 tcgggctctc cctcctcctg ctcggccagg acaccggcgg caccgcgcgc tccggcctgc
     7381 tggtcggcgc gttctccgcc gctctgatga tcgcgacccc cctgctcggc cgcgccctcg
     7441 acacctttcc tcccggacgg gtgctctccc tggccgcgac cgcccagtgc gcggcgctcg
     7501 ccctcctggc gatctgctgg tcgacctccg agccggagac cggcctgctc ctcacgggcg
     7561 cgctcctcgc gggcgcgtcc ctgcccccgg ccgggcccgc actgcgggtc gcgctgcccg
     7621 tcttcgtccc cgacgacctg ctgaccaccg gtttcaccgc cgaggccgtc atcctggaga
     7681 ccgtctacat catcggcccg ctactggtcg gcgggctcgg cgcggtcgcg ggctggctgc
     7741 cgctcgcggc ggcgggcggc tgcaccgccg ccggggtcgc ggtgatgctg tccagcggac
     7801 gcctcgccgg cgcctgcgtc gacaccccgc ccgcccgcgc cctgtggacg ctcggcccgc
     7861 tcgccgacgg cggcgtccgc cggctgctgc tcggcgcgtt ccttttcctg cccgcgctcg
     7921 cggtggtcga agtgggcgtc gccgccgtcg cgggagagcg cgaccgcccc gaactgctcg
     7981 gcgtcgtgat ggcgttctcc gccgtcgcga gcctggcggg cggcctcctg ttcggcctgc
     8041 gccgctggcc ggggtcgctg gcctcccatg tggcggtgct cgcgggctgg ttcgcggcgg
     8101 cgtccgtcct cggcgccctg gccccgggcc cctggccggc cggcgcggcg gtcgtcctgg
     8161 gcagcctcgt cgtcgccccc gccctcgccc tcctcggccg gatcggcggc gtcgtcgccc
     8221 ccgagggccg cgccgccgag acctacacct ggctgtccac catgaacaac tgcggccagg
     8281 cggccaccct ccccctggcc ggcctcctgg ccgaacgcag cggccccgaa ggcgccttcc
     8341 tcctggcagc caccctggcc ggcaccgcag ccctgttcat cctgaacaac cgccgccacc
     8401 tcgcacccac caccacgtcc cgccccagag aaacagtaag aacctaaccc cagcccccac
     8461 accgaactgc ctaagccacc ctcaccgcaa gccatcccta ccgccaccga acccaacctg
     8521 acccgccgca ccccttgccc tgcccccaac accaagcccg ccacaacgcg cccccgtcgc
     8581 caaccacccc acccaagccg aacccgccgc aggcgcacct gccctcacac ggtcctccca
     8641 agccgagcgc acgccaccga agccgccacg ccgcgcccgc tgcaagagga ccgccgtctg
     8701 aggacccaag ccaaccctgc tgagccgggc ccgctgcgat gcaggccatc cgccagacgg
     8761 gatccctcct cgcgctcgtc gcgccgaacc cgctacgcct aacccgccgc aggcgcccct
     8821 gccttcggac gcgccgccca agccaagccc attccaaacc gaggcgccta tgccgagcgc
     8881 gctgcaccga agccgttaca ccgagcccgc tcgatggtcc tggcgcagcg ccgcccgcga
     8941 cagcgccacc cgctggagca gcgggacggc cgccgccagc gcctcctcgc cgagcaccgc
     9001 cgccgtcggg acggcgcgct ccgggccgcc cgcagcgcca gcgtcggggt tgccgccggg
     9061 cagctcctca agccagacct gctgagtcag ccccgctgca agtcatgcca agcgcctgat
     9121 gggcgcgccc ttacccccgc ccactgcact ggactcacca tgccgagctc gctgcaagcg
     9181 gccccgtcgt cagacagccc gccccaagcc gagtccgccc caaggcgagc ccgccttagc
     9241 cgtatcgcct acgccgagcg cgccccaagc cgaatcgact acgccgaggc attgcgcctg
     9301 agtggctgcg gcgcgatcgc tgcaacaggg ccgccgttgg acggcccttt cccaggctgg
     9361 gcccgctgcg caagcccgct gtagggcgga ccggttgccg gacacgggcg tcccagccga
     9421 gccgcgtacg ccgaggcgct ggcccgcggc ggcgagccgc aggaaagggt gcacagcgga
     9481 cggccccgcc cagccgagcg cgctgcgccg agccctgctg cgaagcggac cggctgccgg
     9541 acgggcccgc cctgggcgag gtcgccgttg ggcgggcccg cgatcgactg cggtctagat
     9601 caggccctgc gcgagcatcg cgtcggcgac gcgctcgaag cccgcgatgt tggcgccgac
     9661 gacgtagtcg ccgggcgcgc cgtaccgctc ggcggtctcg aagcaggtgt cgtggatgga
     9721 cgtcatgatc cccgacagct cggactcgac ccgctcgaac gtccacgact cgcggcccgc
     9781 gttctgctgc atctccagcg cgctcaccgc gacgccgccc gcgttggcgg ccttgcccgg
     9841 cccgaacgcg acgcccgcgc cctggaacac gtgcaccgcg ccgggcgtcg ccggcatgtt
     9901 cgcgccctcc gagacggcct tgacgccgtt gcggacgagc accgccgcgg cgctctcgtc
     9961 cagttcgttc tgcgtcgcgg acgggagcgc gatgtcggcg ggcacgtccc agacgctgcc
    10021 gcccgccaca tactgcgccg agtcgccgcg ctgctcggca tagtcggaga cgcggccccg
    10081 gtcgacctcc ttgaccttct tgagcaggtc gaggtcgatg cccttctcgt ccaccacgta
    10141 gccgccggag tcggagatcg tgacgacgtt cgccccgagc gcctgcgcct tctcgatggt
    10201 gtagatcgcc acgttgcccg agccggacac gacgacctgc tggccgtcga ggtcctcccc
    10261 gcgccgccgc agcatctcgg cggtgaacat gacgttgccg tagccggtgg cctccgtccg
    10321 gcccgcggat ccgccccacg gcaggccctt gccggtgagc acgcccgcct cccagcggtt
    10381 ggtgacccgc cggtactgcc cgaacaggta gccgatctcg cgtccgccga ccccgatgtc
    10441 gcccgccggg acgtcggtgt ggtcgccgat gtgtcgctgc agctcggtca tgaacgactg
    10501 gcagaaccgc atgacctcgg cgtcggagcg cccgtgcggg tcgaagtcgc tgccgccctt
    10561 gccgccgccg atgccgagcc cggtcagcgc gttcttgaag atttgctcga agccgaggaa
    10621 cttcaccacg ccgaggttca ccgtcgggtg gaaccgcagc ccgcccttgt acggcccgag
    10681 cgcgctgttg aactcgaccc ggaacccgcg gttgacgtgg atctcgcccg cgtcgtcctg
    10741 ccatgggacg cggaagacga tctgccgctc cggctcgatg acccgctcca ccatcttcgc
    10801 ggccgcgagc ccgggtcgcg cggccagcac gggcgggagt gactccagca cctcgtggac
    10861 ggcctggtgg aactcaggct cgcccgggtc gcggcgcagg acccgctcgt aggactcctc
    10921 cagcagggcc agtgtctccg gcggtgcctg gaggcggggg gacaagggca taggtgattc
    10981 ctttcgcaga acaacaacct ttgtacctgc ccgggagcac catcctcgca ccccgcgatc
    11041 atccgcgcgg tgcgaacgcc ggcctgcttc cccttgaccg gatgatctga caaccggacc
    11101 accccagggc gccatgcgcc ctactctgtc gggatgatcc ccaatgcggt tgagtcctcc
    11161 gcaatcgccc gactcggctg gttggacgcg ctgcggggat gggcggcgat cacggtcgcg
    11221 ctccatcacg cgagctatgt ctacatcccg gacgtgcaga ggtggatggc cgaacgcttc
    11281 gaccccggcc gctacggcgt cctcgtgttc ttcctggtca gcggctatat cgtaccggcc
    11341 tctctggagc gccgcggcag cgtccggggc ttttggatcg gacgcttctt ccgcatctac
    11401 cccatgctcg cgctgtgctg cctggcggcg gtgctcccct tcatcctcgg catcctcggg
    11461 ctgcgcgcgg ggctggaaca gtacgacccg gtgacagccg tcctggcgca tctgaccatg
    11521 ttgcaggatc tcctcgccgt ccccaatgcc atcaacgtcc tgtggacgct gacctacgag
    11581 atggccttct acctccttat cgtcgggttg ttcgtcgtcg gcgggcaccg ccgctccgcg
    11641 ccggtcgcct ccggattcgc actggccgcg gtgctcgtgg gcggcctcat cccctccacc
    11701 ctgctctcgc gcaccgcggg catcggcccc ctcgtcgccg ccgtcaccat cggtctggtc
    11761 atctcgatcg ccgccgccgt gtcggaccgc cccggcctgc gcgtcgtcgg cggcgtcctc
    11821 ggcggcctgc tggccgccgc gctggcggcc gccaacagcc gcgtctacgc ctgggagggg
    11881 ctgaccctcc tagcgatcat gttca
//
