LOCUS       NZ_WOFH01000001        10903 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Actinomadura litoris strain NEAU-AAG5 Scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_WOFH01000001
VERSION     NZ_WOFH01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura litoris
  ORGANISM  Actinomadura litoris
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 1015624)
  AUTHORS   Cao,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2019) Northeast Agricultural University, College
            of Life Science, No. 600 Changjiang Road, Harbin, Hei Longjiang
            150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009733595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 03:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,194
            CDSs (total)                      :: 8,115
            Genes (coding)                    :: 8,044
            CDSs (with protein)               :: 8,044
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 60 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:00:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 230685
            Orig. end                         :: 241588
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WOFH01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10903
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10903
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10903
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5903](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5903)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            285..980
                     /locus_tag="GNZ18_RS01070"
                     /old_locus_tag="GNZ18_01075"
     CDS             285..980
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS01070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_01075"
                     /product="S1 family peptidase"
                     /protein_id="WP_156214175.1"
                     /transl_table=11
                     /translation="MRLRISSAAATAAACAIATLAAPAPGAMASPASPASPAAPPSAVG
                     GQTVYASDGSPCTLGFNVRRSDGYYFLTAGSCARQDQRVYADSAKTTALGTVVARTPLG
                     VGLVRYVEPQAERPGGVDTHPGSQHIKGAGHVPIGQQICRSSPVSGLRCGKLKAVDLSV
                     MLPDGTMGTIAQTDACGLPGDAPGAPYFAGGTALGVEFQGSGACEGGENSYYQQIDEIL
                     VQLGGGEVY"
     gene            complement(1028..2632)
                     /locus_tag="GNZ18_RS01075"
                     /old_locus_tag="GNZ18_01080"
     CDS             complement(1028..2632)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013714.5"
                     /locus_tag="GNZ18_RS01075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01080"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_156214176.1"
                     /transl_table=11
                     /translation="MTEHTRRNVLRGGLAATAAGVAAPALGAVPAAAAGPAAPPPPASE
                     VGRGDPRFPSLASRGYNRRFVGDPDHVWVVGTTEDVVTAVQRAVDTGRRVAVRGGGHGF
                     EGFVGDPAVRVVIDTSAMTDVYYDARRRAFAVEAGALLGDVYRRLYLGWGVTIPAGWCP
                     TVGAGGHVPGGGFGPLSRTMGLAVDHLHAVEVVVVGRDGRARSVVATSGASDPNRDLWW
                     AHTGGGGGTFGVATRYWFRTPDARGDDPSRLLPAPPSTALTFSVDWDWSRLDRAAFERL
                     VRNHGTWVERNSAPGSPAAALYSELQLTRPAAGTIGMIGQVAAVAGASAAERLLDGHIA
                     AISEGVPVAPVRKAANQPWLAAALAGSPGDPGPRYRLKVKSAYLRRRLTDRQIGVLHHH
                     LTRTDYDHPGAILSLYTFGGRVNAVRPDATAAPHRDTIMKMFFINGWEDAADDARHIAY
                     AREVYRDLYADTGGVPAAGDGAFINYPDTDLADPALNGGAPWHVLYFRGNYPRLQRIKA
                     KWDPRDVFRHALSVRPV"
     misc_feature    complement(2639..2653)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.12"
                     /tool="antismash"
     misc_feature    2646..2664
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.59"
                     /tool="antismash"
     gene            complement(2733..4193)
                     /locus_tag="GNZ18_RS01080"
                     /old_locus_tag="GNZ18_01085"
     CDS             complement(2733..4193)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024306.5"
                     /locus_tag="GNZ18_RS01080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01085"
                     /product="ferritin-like protein"
                     /protein_id="WP_156214177.1"
                     /transl_table=11
                     /translation="MTTTTTRTKARKPASKAKAKTQPKTQPKTQAKTQAKTQPRTKARA
                     ARKAGDRLVRTRPIRQLDPAAGIRTVDDLADHLYVAAQVELSTIPMYLFSAYSIRTRGH
                     SQWAAPRGVLRTLVGISIEEMLHLALVRNLMVAIGYGEDISFYDEEFIPSYPSTMLNRY
                     NPDDPDGPEIILSLDRLSKEHVGTFRRVEMPDDIDLGAETFARHPRDVGQYTSLGEFYR
                     AIEQGFIDLEDEIEWAVEDARKQYKRGFWNEFGNGKPIRVHDLATARQALRIIIEQGEG
                     TITDHKKIEIRPGVEDYTHYEKFVRVQEGIEGIGAVDGGPEHRIDIDDPEATWPVVADP
                     LVADFDDQPGVQSLMTLFNAAYCYTLCLLDETYAHSTDDVRTKKVPGTDREEMFSHRYR
                     LERNDVAVMQGILYPIAQALVTTPIPDGPYKGRHAAPSFEFYDFDADKQERTKKEQLID
                     LCAEAIEHFPVLGGPDGVERQIRLLVDI"
     gene            complement(4190..5008)
                     /locus_tag="GNZ18_RS01085"
                     /old_locus_tag="GNZ18_01090"
     CDS             complement(4190..5008)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="GNZ18_RS01085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01090"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_156214178.1"
                     /transl_table=11
                     /translation="MPEPVAGLAGTQRWMQAAILGDGAADGVERTLTASSSLTARERLG
                     IYRRGYRLRLLENMRGLHPGLTRLLGEEMFDRFALDYLDARPSRTYTLTRLDEGFADHL
                     EATRPDGAAHREQWPDLIIDLARLERIITEVVEGPGTEGGPVLRPDDLPAALDLSRGCD
                     LRLETAPCLRLPTFAFPVNEYLAAVRRGEDPEPPAPRPVALAVSRRDYRVTMRELDPAA
                     HRALRALAGGAAVGAVLADPSAEALRRWAGLGYFTRAHRAPEHLEERARR"
     gene            complement(5001..5903)
                     /locus_tag="GNZ18_RS01090"
                     /old_locus_tag="GNZ18_01095"
     CDS             complement(5001..5903)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="GNZ18_RS01090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01095"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_308165886.1"
                     /sec_met_domain="DUF692 (E-value: 2.3e-94, bitscore: 311.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWADTAADTATGTPAGTAAGMPGFGIGLRGPHMDHVRRHRPDVG
                     FFEVVSENFMDCAGGRRRALEAIAEHYPIALHGVSLSIGGTDPLDLGYLARLRGLAAAV
                     RAPLVSDHVCWTGVMGRNTHDLLPLPFTEAVLAHVVERVRIAQEFLGRRLVLENPSTYV
                     GFTASAMPEQEFMTRLAEEADCGLLLDVNNVYVSAANHDFDPVDYLDALPMERVEQIHL
                     AGHADHGTHLIDTHDRPVSDAVWDLYRLVTERAGPIPTLLEWDDRLPEFPVLEAELGKA
                     RLAAAGRDPVSALQGVPDA"
     gene            complement(5900..6592)
                     /locus_tag="GNZ18_RS01095"
                     /old_locus_tag="GNZ18_01100"
     CDS             complement(5900..6592)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS01095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_01100"
                     /product="hypothetical protein"
                     /protein_id="WP_156214179.1"
                     /transl_table=11
                     /translation="MKTQVDLQFGEFEGGMAKVRREHNGHDRAAGPEVVSSQEYAVEQH
                     YDASGRSFGGASCGGPAEGLEQHVCMGLNACNAFDVDGGAVMAGTGNCATVRHVCHGEG
                     QCRGQGGCGYAGGDDQQWRPGEQACRFNGSCASPLNVSRVFSAGPMKGRSVWLQARRLM
                     EDRMYRAGLAYGPSPGDGIPDDLLPEYDVDDGEDLVGPVGRRGAASGDGARRKTAGKAR
                     KAAPRKAR"
     misc_feature    6621..6634
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.67"
                     /tool="antismash"
     misc_feature    6630..6645
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     misc_feature    complement(6658..6672)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.0"
                     /tool="antismash"
     misc_feature    complement(6680..6696)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    6751..6765
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.3"
                     /tool="antismash"
     gene            6789..7442
                     /locus_tag="GNZ18_RS01100"
                     /old_locus_tag="GNZ18_01105"
     CDS             6789..7442
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GNZ18_RS01100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GNZ18_01105"
                     /product="S1 family peptidase"
                     /protein_id="WP_156214180.1"
                     /transl_table=11
                     /translation="MRARIRVIFACVATVMSLLVPGTAAATAPPPSEGGRAIYAANGVR
                     CALGFNVVRSGTYYFLTAGGCAQAGLRIYADPALTVPLGTVAAVTNYKIALVQYVDPKT
                     QRPGTVHTYPGTQEIVTAGAPAAGQRVCRSGPVNHLVCGPVTATNLTINLPDGVINGVA
                     QATVCPGELPGAPYFADTRAVGLEIGRSGSCAGGGSSYFLPVGPILSAFGVAVY"
     gene            complement(7456..8844)
                     /gene="murF"
                     /locus_tag="GNZ18_RS01105"
                     /old_locus_tag="GNZ18_01110"
     CDS             complement(7456..8844)
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541823.1"
                     /locus_tag="GNZ18_RS01105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01110"
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_312874238.1"
                     /transl_table=11
                     /translation="MIPLTLHEIALAVGGTVHGAPAPGPTVTGPVVIDVRDVTPGALFV
                     AVAGERGDGHDLAARAYAFGACAVLGTRAVEGPCVVVDEVVSALTDLAMFVRDMLDLVV
                     IGVTGSVGKTTTKDLLAQILEREAPTVATAGSDGNEIGLPLTILRADRWTRYLVLEMGA
                     ARQGDITHLAWVARPQFGLVLNIGPAHLLTLGGIIGVARAKAELVRALPPAGEGGLAFL
                     NADDAAVASMAGATSAGVVSYGRDAEASVRAGGVTVDGEGRASFILHTPSGAAAVRLGL
                     PGEHQVGNALAAASVADVLGVTTRRIAARLSAAAPRSPNRFQLVERADGITVIDDACHA
                     NPVSMRAALRTLATMSAGRRTIAVLGEMTGQAGDAARHHAAVGRLAAELGVAILVVVGT
                     GEGPDALSEAARRAGVATHALPRRREAVALLRSLLRPADVVLVKASGEIGLSAVATALW
                     NPRS"
     gene            complement(9140..10330)
                     /locus_tag="GNZ18_RS01110"
                     /old_locus_tag="GNZ18_01115"
     CDS             complement(9140..10330)
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_28"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_tran_28_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577442.1"
                     /locus_tag="GNZ18_RS01110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GNZ18_01115"
                     /product="glycosyltransferase"
                     /protein_id="WP_156214182.1"
                     /sec_met_domain="Glyco_transf_28 (E-value: 1.5e-15,
                     bitscore: 53.2, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_tran_28_C (E-value: 2.5e-21,
                     bitscore: 72.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHVVVPQALIDRRSPVRLIVSGGGGGDHTFPAVAVVRAARTAFR
                     HHGLELQPFWVGAERDVEAKVAAEEGVPFTAIPTGGTRRARNRLCRVSAAVGRAPMAVT
                     EAVNLVRGIRPDAVLCTGGYASVPVGLAARFCHRPLLVHEQGVRVGLANRITMRGASRV
                     ALGTEPTMDLLPERLRGRAVVTGNPLRPGLAGGDAGSAPARLRWDGWTPSLPTVYVTGG
                     AIGARRLNELVTAILPELLRYANVFHQAGAELLGPVLAAAEALPPELRPRYLARGFVGE
                     ELADLLALADVAVSRGGCRTVGELTALGKPSVLVLPARAPGDERRRHAEHLAAHGAARV
                     LAGDEATPARLVRELALLLTDPLRRSEMSAMARRLGRPGAAAALTNEVLALCAAAP"
ORIGIN
        1 cgccgacgaa ctgctgctca ccaccatcac ccacgaccac gccgaccgcg tccgctcctt
       61 cgaactgatc gccgaggcat ggacgcgcag ctcggaacgg acgtccgcaa acgcgcccgg
      121 cgcgggctga gctccgctga ccggcgcctt ccggccgcct gccccgtagt tgatatacgg
      181 tcggcggccg tcttggcagg ttccccccct tgccggggcc cgcgcccggc cggactattc
      241 cggcaaccca ggaccctggg cgtccatccc cgcaggagac ccccgtgaga ctccgcatct
      301 cctcggcggc cgcgaccgcc gccgcctgcg ccatcgccac gctcgccgct cccgccccgg
      361 gcgcgatggc ctcacccgcc tcccccgcct cccccgccgc cccgccctcc gccgtcggcg
      421 gccagaccgt ctacgcgagc gacggctccc cgtgcacgct cggtttcaac gtccgcagga
      481 gcgacggcta ctacttcctc accgccggtt cgtgcgcgcg gcaggaccag agggtctacg
      541 ccgactccgc caagaccacc gcgctcggca ccgtcgtcgc gcgcacgccc ctcggcgtcg
      601 ggctcgtccg ctatgtcgaa ccgcaggcgg agcgtcccgg cggcgtcgac acccaccccg
      661 gttcgcagca catcaagggc gcgggccacg tgcccatcgg ccagcagatc tgccgcagca
      721 gcccggtctc cgggctgcgc tgcggcaagc tgaaggcggt cgacctgagc gtgatgctgc
      781 ccgacggcac gatgggcacc atcgcgcaga ccgacgcctg cgggctgccc ggcgacgccc
      841 ccggcgcgcc ctacttcgcc ggcgggaccg cgctcggcgt ggagttccag ggctccggcg
      901 cctgcgaggg cggcgagaac tcctactacc agcagatcga cgagatcctt gtgcagttgg
      961 gaggcggcga ggtctactga gcacgtcccg gtcccgacca ccggccccgg agccgcgccg
     1021 cgcgcgatca cacggggcgg acggacagcg cgtggcggaa gacgtcgcgc ggatcccact
     1081 tcgccttgat ccgctggagc cgcgggtagt tgccgcggaa gtacaggacg tgccacggcg
     1141 cgccgccgtt cagcgccggg tccgcgagat cggtgtcggg gtagttgatg aacgcgccgt
     1201 cgccggccgc gggcacgccg cccgtgtcgg cgtagaggtc gcggtagacc tcgcgcgcgt
     1261 aggcgatgtg ccgggcgtcg tcggcggcgt cctcccaccc gttgatgaag aacatcttca
     1321 tgatcgtgtc gcggtggggc gccgccgtcg cgtccgggcg gacggcgttg acccgcccgc
     1381 cgaaggtgta gaggctgagg atcgcgccgg ggtggtcgta gtcggtgcgg gtcaggtggt
     1441 ggtgcaggac gccgatctgc cggtcggtca ggcggcgccg caggtaggcc gacttcacct
     1501 tcagccggta ccgcggcccc gggtcgcccg gcgacccggc cagcgcggcc gcgagccacg
     1561 gctggttcgc cgccttcctg acgggtgcga ccggcacgcc ctcgctgatc gcggcgatgt
     1621 ggccgtccag cagccgctcg gcggcggacg cgcccgcgac ggcggcgacc tggccgatca
     1681 tgccgatcgt cccggccgcc gggcgggtga gctggagttc gctgtagagc gcggccgccg
     1741 gggagcccgg cgcgctgttg cgctccaccc aggtgccgtg gttgcggacg agccgctcga
     1801 acgcggccct gtccagcctg ctccagtccc agtcgacgga gaacgtcagc gccgtggacg
     1861 gcggcgcggg cagcagccgg gacgggtcgt cgccgcgcgc gtccggcgtc cggaaccagt
     1921 agcgggtggc gacgccgaac gtcccgcccc cgccgccggt gtgcgcccac cacaggtccc
     1981 ggttcgggtc ggacgccccg ctcgtcgcca ccacgctccg ggcccgcccg tcccggccga
     2041 cgacgaccac ctcgaccgcg tggaggtggt cgacggcgag gcccatcgtc cgcgacagcg
     2101 ggccgaagcc gccgccgggc acgtgcccgc cggcgccgac ggtcgggcac cacccggcgg
     2161 ggatcgtcac gccccatccc aggtagagcc tgcggtacac gtcgccgagc agcgcgcccg
     2221 cctccaccgc gaaggcgcgg cggcgggcgt cgtagtagac gtcggtcatc gcggaggtgt
     2281 cgatgacgac gcgcacggcg ggatcgccga cgaagccctc gaagccgtgg ccgccgccgc
     2341 ggaccgccac cctcctgccg gtgtcgacgg cccgctgcac cgccgtgacc acgtcctcgg
     2401 tcgtcccgac cacccacacg tggtcgggat cgcccacgaa ccgccggttg tagccgcgcg
     2461 aggccaggga cgggaagcgc gggtcgccgc gtccgacctc cgacgccgga ggcggcggcg
     2521 cggcgggccc ggccgccgcg gccggtaccg cgcccagggc gggcgcggcg acccccgccg
     2581 ccgtggccgc cagcccgccc cgcaggacgt tcctccgcgt gtgctcggtc atccggactc
     2641 ctcagatcgc gaatgaccac caacgtaggg acggcgcggg atgcggtcag tgggcctggc
     2701 cccacctcat cggtaggcgc aacccccgga tgtcagatgt cgacgagaag ccggatctgc
     2761 cgttcgacgc cgtccgggcc gccgagaacg gggaaatgct cgatggcctc cgcgcacagg
     2821 tcgatgagct gctccttctt ggtgcgctcc tgcttgtccg cgtcgaagtc gtagaactcg
     2881 aacgagggcg ccgcgtgccg ccccttgtag gggccgtccg gaatcggcgt cgtgaccagc
     2941 gcctgcgcga tggggtagag gatcccttgc atcaccgcga cgtcgttgcg ttcgagccgg
     3001 taccggtggc tgaacatctc ctcgcggtcg gttcccggga ccttcttcgt cctcacgtcg
     3061 tcggtggaat gcgcgtaggt ctcgtccagc aggcacagcg tgtagcagta ggccgcgttg
     3121 aacagcgtca tcaggctctg gacgccgggc tggtcgtcga agtcggccac cagcgggtcg
     3181 gcgacgacgg gccaggtcgc ctccgggtcg tcgatgtcga tccggtgctc ggggccgccg
     3241 tcgaccgcgc cgatgccctc gatgccctcc tggacgcgga cgaacttctc gtagtgggtg
     3301 tagtcctcga cgcccggcct gatctcgatc ttcttgtggt cggtgatcgt gccctcgccc
     3361 tgctcgatga tgatccgcag cgcctgccgg gcggtggcga ggtcgtggac gcggatcggc
     3421 ttgccgttgc cgaactcgtt ccagaacccg cgcttgtact gcttgcgcgc gtcctccacc
     3481 gcccactcga tctcgtcctc caggtcgatg aacccctgct cgatcgcgcg gtagaactcg
     3541 cccaggctcg tgtactggcc gacgtcccgc gggtgccgcg cgaacgtctc cgcgccgagg
     3601 tcgatgtcgt cgggcatctc gacccggcgg aaggtgccga cgtgctcctt ggagagccgg
     3661 tccaggctga ggatgatctc cgggccgtcc gggtcgtcgg ggttgtagcg gttcagcatc
     3721 gtgctcggat acgacgggat gaactcctcg tcgtagaagg agatgtcctc gccgtacccg
     3781 atggcgacca tcaggttgcg cacgagcgcc aggtgcagca tctcctcgat cgagatcccg
     3841 accagcgtgc gcagcacgcc ccgcggcgcc gcccactgcg agtgcccgcg cgtccggatc
     3901 gagtacgcgg agaacaggta catcgggatc gtggacagct ccacctgcgc ggcgacgtac
     3961 aggtggtcgg ccaggtcgtc cacggtccgg atccccgccg ccgggtcgag ctggcggatc
     4021 ggtctcgtcc gcaccagccg gtcgcccgcc ttccgcgcgg cgcgcgcctt cgtcctgggc
     4081 tgcgtcttgg cctgcgtctt ggcctgcgtc ttgggttgcg tcttgggctg tgtcttggcc
     4141 ttcgccttgg acgcgggctt gcgcgccttc gtccgggtcg tggtggtggt catcgtctcg
     4201 ccctctcctc aaggtgttcg ggtgcgcggt gggcgcgggt gaagtagccg agtccggccc
     4261 agcggcggag cgcctcggcg gacgggtccg cgaggaccgc gccgaccgcc gcgcccccgg
     4321 ccagcgcccg cagcgcccgg tgcgcggcgg ggtcgagctc gcgcatcgtg acccggtagt
     4381 cgcggcggct caccgccagc gcgaccggac gcggcgcggg cggctccggg tcctcgccgc
     4441 gccggacggc cgccaggtac tcgttgacgg ggaaggcgaa cgtcggcaac cgcaggcacg
     4501 gcgccgtctc cagacgcagg tcgcagcccc gcgagaggtc gagggccgcc gggaggtcgt
     4561 ccgggcgcag gacgggcccg ccctcggtgc cggggccctc gacgacctcc gtgatgatcc
     4621 gctccagccg ggccaggtcg atgatcaggt ccggccactg ctcgcggtgc gccgcgccgt
     4681 ccggcctggt cgcctccagg tggtcggcga acccctcgtc gagccgggtg agcgtgtacg
     4741 tccgggacgg gcgcgcgtcg aggtagtcga gggcgaaccg gtcgaacatc tcctcgccga
     4801 gcagccgcgt cagcccgggg tgcaggccgc gcatgttctc cagcagccgg agccggtagc
     4861 cgcgccggta gatcccgagc cgctcccgcg cggtcagcga cgacgacgcc gtgagagtcc
     4921 tttcgacgcc gtcggccgcg ccatcgccga gaatggcggc ctgcatccac cgctgggtcc
     4981 cggccagccc cgccaccggt tcaggcatcc ggtacgccct ggagcgccga gaccgggtcg
     5041 cgtccggcgg cggcgagcct ggccttgccg agttcggcct caaggacggg gaactccggc
     5101 agccggtcgt cccattccag cagcgtggga atcggcccgg cgcgctcggt cacgagccgg
     5161 tagagatccc agacggcgtc ggagaccggg cggtcgtggg tgtcgatgag atgcgtcccg
     5221 tgatcggcgt gcccggccag atggatctgc tcgacgcgct ccatcggcag cgcgtcgaga
     5281 tagtcgaccg ggtcgaagtc gtggttggcc gccgagacgt agacgttgtt gacgtccagc
     5341 agcagcccgc agtcggcctc ctcggccagc cgggtcatga actcctgctc gggcatcgcg
     5401 gacgcggtga acccgacata ggtgctcggg ttctccagca ccagcctgcg gccgaggaac
     5461 tcctgcgcga tccggacgcg ttccaccacg tgcgccagca ccgcctcggt gaaggggagc
     5521 ggaagcaggt cgtgggtgtt gcggcccatg acccccgtcc agcagacgtg gtcggacacc
     5581 agcggcgccc gcaccgccgc ggccagcccg cgcagccgcg ccaggtagcc gaggtcgagc
     5641 gggtcggtgc cgccgatcga cagcgagacc ccgtgcagcg cgatcgggta gtgctcggcg
     5701 atcgcctcca gggcgcggcg gcggccgccg gcgcagtcca tgaagttctc cgagaccacc
     5761 tcgaagaagc cgacgtccgg gcggtgccgc cggacgtggt ccatgtgcgg cccgcgcagc
     5821 ccgatcccga accccggcat gcccgcggcg gtgcccgctg gggtgcccgt tgcggtgtcc
     5881 gcggcggtgt ccgcccaggt caccgcgcct tcctcggcgc ggccttccgg gccttcccgg
     5941 cggtcttgcg gcgggcgccg tccccggacg cggcgccgcg ccgccccacc gggcccacca
     6001 ggtcctcgcc gtcgtcgacg tcgtactcgg gcagcaggtc gtcggggatc ccgtcgcccg
     6061 gggacgggcc gtaggcgagg cccgcccggt acatccggtc ctccatgagc cgccgggcct
     6121 gcaaccacac gctcctgccc ttcatcggcc cggcgctgaa cacgcggctg acgttgagcg
     6181 ggctcgcgca gctcccgttg aaccggcacg cctgctcccc ggggcgccac tgctggtcgt
     6241 cgcctccggc gtagccgcag ccgccctggc cgcggcactg cccctcgccg tggcagacat
     6301 ggcggacggt cgcgcagttc ccggttccgg ccatgacggc gccgccgtcg acgtcgaagg
     6361 cgttgcacgc gttcaggccc atgcacacgt gctgctccag gccctcggcg ggcccgccgc
     6421 agctcgcgcc gccgaaactg cgcccgctcg cgtcgtagtg ctgctcgacc gcgtactcct
     6481 gcgaggacac cacctccggg cccgccgccc ggtcgtgccc gttgtgctcg cgcctcactt
     6541 ttgccatgcc gccctcaaat tcaccgaatt gcaggtccac ctgcgtcttc acccgagacg
     6601 aagatcagta agcgcgcggc tcgacttatt acgacacgtg gaaacggcat gcgcccacgt
     6661 tgaccgaaaa ttggcgatca cctgatgttg atacaaggcg gtgaccaaca ttggcacttc
     6721 ccccccttgc cggtggccgc gcttttccgc actattccgg aaagtcagcc ccccggcagg
     6781 aggcccccgt gagagcccgc attcgtgtga tcttcgcgtg cgtcgcgacc gtcatgagcc
     6841 tgctcgtccc cggcacggcc gcggccaccg ccccgccgcc gtccgagggc gggcgggcga
     6901 tctacgcggc caacggcgtg cgctgcgccc tcgggttcaa cgtggtcagg agcggcacct
     6961 actacttcct caccgcgggc ggctgcgcgc aggccggcct gcggatctac gccgacccgg
     7021 cgctgaccgt cccgctcggc acggtcgccg cggtcaccaa ctacaagatc gccctcgtcc
     7081 agtacgtgga cccgaagacg cagcggcccg gcaccgtcca cacctacccc ggcacgcagg
     7141 agatcgtgac ggccggggcc ccggccgccg gccagcgggt ctgccgcagc ggccccgtca
     7201 accacctggt ctgcgggccc gtcacggcga ccaacctgac catcaacctc cccgacggcg
     7261 tgatcaacgg cgtggcgcag gcgaccgtgt gcccgggcga actcccgggc gcgccctact
     7321 tcgccgacac cagggcggtc gggctcgaaa tcggcaggtc cggtagttgc gccggcggcg
     7381 gctcgtccta tttcctgccg gtcggcccca tcctcagcgc cttcggcgtc gcggtgtact
     7441 gaccgcggtc ccccgctagg agcgcgggtt ccacagcgcg gtcgcgaccg cggacaggcc
     7501 gatctcgccc gacgccttca ccaggacgac gtcggccggg cgcagcaggc tccgcagcag
     7561 ggccaccgcc tcccggcggc gcggcagcgc gtgggtggcc acgcccgccc ggcgggcggc
     7621 ctcggacagg gcgtcggggc cctcgccggt cccgaccacg acgaggatcg cgacgcccag
     7681 ttcggcggcc agcctgccga cggcggcgtg gtgccgcgcc gcgtccccgg cctggccggt
     7741 catctcgccg aggacggcga tcgtccggcg tccggccgac atcgtcgcca gcgtgcgcag
     7801 cgcggcgcgc atggagacgg ggttggcgtg gcaggcgtcg tcgatgaccg tgatgccgtc
     7861 ggcgcgctcg acgagctgga accggttcgg cgagcgcggg gcggcggcgc tcagccgcgc
     7921 cgcgatccgg cgggtcgtga cgccgaggac gtcggcgacg ctcgcggcgg ccagcgcgtt
     7981 gccgacctgg tgctcgccgg gcagcccgag ccgcacggcc gccgcgcccg acggggtgtg
     8041 caggatgaac gacgcgcggc cctcgccgtc gaccgtgacg ccgcccgccc ggacggacgc
     8101 ctcggcgtcg cggccgtagg acacgacgcc cgccgacgtc gcgcccgcca tcgacgccac
     8161 ggccgcgtcg tcggcgttga ggaaggccag cccgccctcc cccgccgggg gcagcgcccg
     8221 caccagctcg gccttcgccc gcgcgacgcc gatgatcccg ccgagcgtga gcaggtgcgc
     8281 gggcccgatg ttcagcacca gtccgaactg cgggcgcgcc acccacgcga ggtgggtgat
     8341 gtcgccctgg cgggcggccc ccatctccag gacgaggtag cgcgtccacc ggtcggcgcg
     8401 caggatcgtc agcggcagcc cgatctcgtt gccgtcggag ccggcggtgg cgacggtcgg
     8461 cgcctcgcgt tcgaggatct gggcgagcag gtccttggtc gtggtcttgc cgaccgagcc
     8521 ggtgacgccg atgacgacga ggtccagcat gtcgcggacg aacatggcga ggtcggtcag
     8581 cgcggagacc acctcgtcca cgacgacgca cggcccctcg acggcgcggg tgccgagcac
     8641 cgcgcacgcg ccgaaggcgt aggcgcgcgc ggcgaggtcg tgcccgtcgc cgcgctcccc
     8701 ggcgacggcg acgaacagcg cgccgggggt gacgtcccgc acgtcgatga cgacgggccc
     8761 ggtgacggtg ggaccggggg cgggggcgcc gtgcaccgtt ccgccgaccg ccagcgcgat
     8821 ctcgtggagg gtcagcggga tcacggcacc ggccgcgctg acccgcgatg cgcaccggcc
     8881 ccgtccgcgg cggcctcggc gaccgccgcg gacgggtccg gcctccaggt accgtcccgc
     8941 tcgaccccgc cgacggcggg acggtactcc cccctccgga ggcccatgaa tgcctgcatg
     9001 atctcccgtc cggccccggc gccacccctt agcgccagat cgtaaaacgc cccgaacaca
     9061 ccgggatacc gggcattcgg cccggaccga ccggggtctc cgcccccgcg caccgggcag
     9121 tcccgggccc cgcgccaggt cacggcgccg ccgcgcagag ggcgaggacc tcgttggtca
     9181 gcgcggcggc ggcgccgggc cggccgaggc ggcgcgccat cgccgacatc tccgagcggc
     9241 gcagcgggtc ggtcagcagc agcgccagct cccgcacgag ccgggcgggc gtcgcctcgt
     9301 ccccggccag cacgcgggcg gcgccgtgcg cggcgaggtg ctcggcgtgg cggcgccgct
     9361 cgtccccggg ggcgcgggcg ggcaggacga gcaccgacgg cttcccgagc gcggtcagct
     9421 cgccgaccgt ccggcagccg ccgcgcgaca ccgcgacgtc ggcgagcgcg agcaggtcgg
     9481 ccagctcctc gccgacgaac ccgcgcgcga ggtagcgggg gcgcagctcg ggcggcagcg
     9541 cctcggccgc cgccagcacc ggcccgagca gctcggcgcc cgcctggtgg aacacgttgg
     9601 cgtagcgcag cagctcgggc aggatcgcgg tgaccagctc gttgagccgg cgcgccccga
     9661 tcgcgccgcc ggtgacgtag acggtcggga gcgagggcgt ccagccgtcc cagcggagcc
     9721 gcgccggcgc ggagcccgcg tcgccgccgg cgaggcccgg gcgcagcggg ttgccggtca
     9781 ccaccgcccg cccccgcagc cgctcgggca gcaggtccat ggtcggctcg gtgccgagcg
     9841 cgacgcgcga cgccccgcgc atcgtgatcc ggttggcgag ccccacccgc accccctgct
     9901 cgtggacgag cagcggccgg tggcagaacc gcgccgccag cccgaccggc accgaggcgt
     9961 acccgccggt gcacagcacg gcgtcgggcc ggatcccccg gacgaggttg accgcctcgg
    10021 tcaccgccat gggcgcccgg ccgacggcgg ccgacacccg gcagagccgg ttgcgggcgc
    10081 gccgcgtccc gccggtgggg atcgcggtga acggcacgcc ctcctccgcc gcgaccttgg
    10141 cctcgacgtc gcgctcggcg cccacccaga acggctggag ctccaggccg tggtggcgga
    10201 acgcggtccg cgccgcccgg acgaccgcga cggccgggaa ggtgtggtcg ccgccgccgc
    10261 ccccgctgac gatgagccgg accgggctgc gccggtcgat cagggcttgg gggacgacga
    10321 catgcgtcat gaccactcca cggggcgggg cgttcgggcg gacagggcgg ggttcaggcg
    10381 gactcaggcg gactcaggcg gacagggcgg cggcgagctg gcgccgcccg ctgatgccga
    10441 gcttgcggta ggcgctggtg aggtactgct ccacggtgcg ctgggtcagc accatctcct
    10501 cggcgatctg gcggttggtg gcgccggcgg cggcgcggtc ggcggcggcg cgctcctgcg
    10561 cggtgagcgc ggtgacgccg ctgagccgcc cgaggtcggg cttgccgccc gcgcggcgca
    10621 ggtggcgggc cgcgcgcagc gacagcggcc gggcgttgat ctcgtgcgcc agcgcgaacc
    10681 cgtcctggag gcgctcgtgg gcctgctcgg cgtcgccccc gcgcagcagc acggcgccga
    10741 ggtcgatgag cgccgccgcg cggaggagcc cggcgtccag cccgtcgagg acggcgacgg
    10801 cctcgcgcgc cgcgtccggg tcggcggcga ccacgccgaa cgcggcgagc gcggcgctca
    10861 gcggacgcgg cgcgccccac cggcgggcca gcgcgacctc ctc
//
