LOCUS       NZ_VTZW01000020        10417 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomonospora sp. PA3 scaffold11.1, whole genome shotgun
            sequence.
ACCESSION   NZ_VTZW01000020
VERSION     NZ_VTZW01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomonospora sp. PA3
  ORGANISM  Streptomonospora sp. PA3
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Streptomonospora.
REFERENCE   1  (bases 1 to 186200)
  AUTHORS   Matroodi,S., Siitonen,V., Baral,B., Yamada,K., Akhgari,A. and
            Metsa-Ketela,M.
  TITLE     Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated
            Halophilic Streptomonospora sp. PA3
  JOURNAL   Front Microbiol 11, 1237 (2020)
   PUBMED   32582127
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 186200)
  AUTHORS   Matroodi,S., Siitonen,V., Baral,B., Yamada,K., Akhgari,A. and
            Metsae-Ketelae,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2019) Department of Biochemistry, University of
            Turku, Turun Yliopisto, Turku 20014, Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2018
            Assembly Method                   :: a5-MiSeq v. MAY-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_000695235.1
            Genome Coverage                   :: 292.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009728995.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 10:59:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,000
            CDSs (total)                      :: 4,917
            Genes (coding)                    :: 4,877
            CDSs (with protein)               :: 4,877
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 8, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 7, 10 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 8 of 40
            Pseudo Genes (incomplete)         :: 35 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            Pseudo Genes (multiple problems)  :: 3 of 40
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:29:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 90809
            Orig. end                         :: 101226
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VTZW01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    190..205
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.82"
                     /tool="antismash"
     misc_feature    195..204
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.86"
                     /tool="antismash"
     gene            complement(418..1257)
                     /gene="ehuA"
                     /locus_tag="FZ103_RS22780"
                     /old_locus_tag="FZ103_22770"
     CDS             complement(418..1257)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590341.1"
                     /locus_tag="FZ103_RS22780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22770"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_156004029.1"
                     /transl_table=11
                     /translation="MQQSESTHSTETAGVQSAQEAPGGEIIRFDKVVKRYGSNVVLNEL
                     DFSVAPGERVTLIGPSGSGKTTILRLLMTLEDIDGGVIWVNGEPLSHMRRGDKLVKANE
                     AHLRSVRKNIGMVFQQFNLFPNMSVRKNITEGPVHSLGVSKDEANQRAEELLDLVGLNE
                     KIDAHPTQLSGGQQQRVAIARALAMRPQILLLDEVTSALDPELVAGVLDVLRNIAQTTD
                     ITMLCVTHEMSFARDVSHRVLMFDAGQVAEEGPPEQIFGDPQEERTKSFLKAVLEHG"
     gene            complement(1244..1882)
                     /gene="ehuD"
                     /locus_tag="FZ103_RS22785"
                     /old_locus_tag="FZ103_22775"
     CDS             complement(1244..1882)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538772.1"
                     /locus_tag="FZ103_RS22785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22775"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_186356724.1"
                     /transl_table=11
                     /translation="MTWDTEFAISIIPRLLEGLAVTIQATVIGYLVAVVLGLFVAILRR
                     IDYIGPVVFVVFEFIRTTPVLVQLLFVFALAPASVEPLTIGVAVLGVHYAAYTAEVYRS
                     GIEGVAKGQWEACRALSLPPWRVWGAVVLPQAIRKVVPALGNYLISLFKDTPYLFAITV
                     GEMLFVANAIGTEAFRSMEAYTLAGLFFLAVSVPSAVIIRRLERRYAAV"
     gene            complement(1879..2604)
                     /gene="ehuC"
                     /locus_tag="FZ103_RS22790"
                     /old_locus_tag="FZ103_22780"
     CDS             complement(1879..2604)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156203.1"
                     /locus_tag="FZ103_RS22790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22780"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_186356725.1"
                     /transl_table=11
                     /translation="MEFFLNRFPLYLDGALITLLMTVLGSAVAFVLAMVIGILGTLRNP
                     VARAVATVYTEAFRGVAALILLFWFAYAVPQITPYQLSPMFAGVLALGLNVGAYGAEVV
                     RAAINAVPRAQTEATIALNMSWARRMWLVVLPQAWAQMLPTFGNLIIELMKASAIVSLI
                     GVADLTQVAEEQRSSSGETVLAFGSALVMYFVIAQVLIFGMRLLERRANRRLGRAPAGG
                     GLLDLLRPPTPGTKVGAAK"
     gene            complement(2614..3528)
                     /gene="ehuB"
                     /locus_tag="FZ103_RS22795"
                     /old_locus_tag="FZ103_22785"
     CDS             complement(2614..3528)
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592139.1"
                     /locus_tag="FZ103_RS22795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22785"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_156004031.1"
                     /transl_table=11
                     /translation="MSNSSPLSHLGRRDLLRVGGVTAAALAAGPGLAACSRTEPAEGQT
                     LLESLREKGSIKAAINNEPPFGFIDDNGKVTGEAPELLRAIVKEMGIDKIEAETVSWDG
                     LIPGLKAGRYDVVAAGMYITPERCQEVAFAEPDYKVLEAFMVPKGNPKNISDYKSVAEN
                     PDIKIAVLNASVEQNYAEGNGVTKEQMTLGDNAVQMLDLLEQGRVDAVGLSTFSLNYQM
                     EQRGMQDDYEVTEGFTPVVDGEEVIPAGGFAFRKENDEFLKEFNKVLADFKESGRLTEI
                     GKPFGFDEAAHPGDLTTEELCSQ"
     misc_feature    3768..3785
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.07"
                     /tool="antismash"
     misc_feature    3835..3855
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.09"
                     /tool="antismash"
     gene            complement(3868..4743)
                     /gene="thpD"
                     /locus_tag="FZ103_RS22800"
                     /old_locus_tag="FZ103_22790"
     CDS             complement(3868..4743)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538769.1"
                     /locus_tag="FZ103_RS22800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22790"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_255430263.1"
                     /sec_met_domain="PhyH (E-value: 5.1e-35, bitscore: 114.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDYPTRKDQAPALLYRKDPTVWGEAASGPMDAATLDSYDAKGYL
                     QFESLITRDELESFRAELTRLSSDDELKKDERVVVERASDEVRSVFEVHKISPVFADLV
                     RDPRLVDRARQILGSDVYIHQSRVNYKPGFSGTDFFWHSDFETWHAEDGMPRMRAVSIS
                     IALTDNYEHNGSLMIMPGSHKTFVSCVGETPDDHYRASLKKQEIGTPDQSSLSILADQH
                     GIDVLTGPAGGAVMFDSNCMHGSNGNITPFPRSNVFIVYNSVENTCEAPFAASKPRPYF
                     LGAQDFTPVK"
     gene            complement(5001..5417)
                     /locus_tag="FZ103_RS22805"
                     /old_locus_tag="FZ103_22795"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290749.1"
                     /locus_tag="FZ103_RS22805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22795"
                     /product="ectoine synthase"
                     /protein_id="WP_156004035.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.5e-55, bitscore:
                     178.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVDGTDADVRTETWRSRRVVLAKDGVGFSFHETVLYAG
                     TETSMWYANHIELVHCIEGEAEVTNDETGETHLITPGTLYLLNGHEHHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGVYPLLSETGERLDTPPQ"
     gene            complement(5539..6804)
                     /gene="ectB"
                     /locus_tag="FZ103_RS22810"
                     /old_locus_tag="FZ103_22800"
     CDS             complement(5539..6804)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-355). E-value: 9.5e-73.
                     Score: 237.0. Matches aSDomain:
                     nrpspksdomains_FZ103_RS22810_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558126.1"
                     /locus_tag="FZ103_RS22810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22800"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_156004037.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.2e-73, bitscore:
                     238.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIFERLESEVRSYCRSWPVVFNEARGSYVYDESGRPYLDFFAGA
                     GSLNYGHNHPELKSKLIDYLMSDAIVHSLDAYSTAKRDFLATFGETILGPRGLDYKVQF
                     PGPAGNNAVEAALKLARKYTGRETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLSHS
                     ATMPFDRYMDGQTPDFLWLRSLLEDSGSGLDAPAAVIVETVQGEGGINVASAEWLRGLQ
                     EVCHEYDMLLIVDDIQMGCGRTGPFFSFETAGIEPDIVTLSKSISGYGLPMALTLFKRE
                     LDVWEPGEHNGTFRGPNPGFVTGAEAMRLFWRDDALQKGTEAKGELLDRGLRAVAEELG
                     PDEAHVRGRGMARGLVLERPGAAKALSREAFERGLLLETSGPEDEVAKLLPALTTTEQE
                     LEEGLEIIADAARAAMKTPQPA"
     aSDomain        complement(5740..6738)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FZ103_RS22810_Aminotran_3.1"
                     /evalue="9.50E-73"
                     /label="FZ103_RS22810_Aminotran_3.1"
                     /locus_tag="FZ103_RS22810"
                     /protein_end="355"
                     /protein_start="22"
                     /score="237.0"
                     /tool="antismash"
                     /translation="NEARGSYVYDESGRPYLDFFAGAGSLNYGHNHPELKSKLIDYLMS
                     DAIVHSLDAYSTAKRDFLATFGETILGPRGLDYKVQFPGPAGNNAVEAALKLARKYTGR
                     ETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLSHSATMPFDRYMDGQTPDFLWLRSL
                     LEDSGSGLDAPAAVIVETVQGEGGINVASAEWLRGLQEVCHEYDMLLIVDDIQMGCGRT
                     GPFFSFETAGIEPDIVTLSKSISGYGLPMALTLFKRELDVWEPGEHNGTFRGPNPGFVT
                     GAEAMRLFWRDDALQKGTEAKGELLDRGLRAVAEELGPDEAHVRGRGMARGL"
     gene            complement(6886..7389)
                     /gene="ectA"
                     /locus_tag="FZ103_RS22815"
                     /old_locus_tag="FZ103_22805"
     CDS             complement(6886..7389)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546867.1"
                     /locus_tag="FZ103_RS22815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22805"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_394350135.1"
                     /transl_table=11
                     /translation="MADGPDLWRLARDTGLDINSPYAYTLWARDFADTSVIARDEAGQP
                     RGFVTGFIRPTRPDTYFLWQVGVDPGFRGRRLARRMLDHIGERIAARGLVHLEATVTPD
                     NAASRALFESFARSRECEAVWTPLFGREHFPADGSEEHEPEDLVRIGPLGRPGTDDKHV
                     RRHT"
     misc_feature    7515..7533
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.17"
                     /tool="antismash"
     misc_feature    7782..7799
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.46"
                     /tool="antismash"
     misc_feature    8156..8170
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     gene            8205..8471
                     /locus_tag="FZ103_RS22820"
                     /old_locus_tag="FZ103_22810"
     CDS             8205..8471
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538765.1"
                     /locus_tag="FZ103_RS22820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22810"
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_186356727.1"
                     /transl_table=11
                     /translation="MNRLYHDSEDWVAQGACRQYDPELFFPVSTTGPGQNDAELAKAIC
                     RTCAVRSECLSWALRAGEPHGVWGGTTPEDRRYMRRQPLPMAS"
     gene            8755..9444
                     /locus_tag="FZ103_RS22825"
                     /old_locus_tag="FZ103_22815"
     CDS             8755..9444
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542313.1"
                     /locus_tag="FZ103_RS22825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22815"
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_156004040.1"
                     /transl_table=11
                     /translation="MNLADPAAASPAQRRRRAARRPGVAAAALLGSLALATATAPAALA
                     HDTLIDSSPQDGAELDTAPDEVVLTFSNDIGQGGNAIVVTGPEGETHEEGPVEIDGPEA
                     AVGLKELPAAGEYTVAYRVVSSDGHTLEDEIAFSVTEAAVPEPSPSPEAESPSAPPTGG
                     DETGGADAEEAPAAQSPTDPMSSLLGPVGGVIAAIAVLALIAILLIRIRNRPGGRDGAD
                     GDGGGDS"
     gene            complement(9500..10261)
                     /locus_tag="FZ103_RS22830"
                     /old_locus_tag="FZ103_22820"
     CDS             complement(9500..10261)
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567879.1"
                     /locus_tag="FZ103_RS22830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_22820"
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_156004042.1"
                     /transl_table=11
                     /translation="MGVAGGEVTVTALTHRGAVRSANEDAVVMGALTIASANMTLPVRC
                     VLPVSEPVILAVADGLGGHAAGEIASEHAVHRMAEMSPRLAGPDDVDVLLKNIDEEIKD
                     HATQHTEFSGMGTTVAGLLLNTDGNYWFNVGDSRTYRLEGTRLRQLSQDDSPPLPPSDD
                     GKPVTTNFITQSLGGSGSTAMVPHVGRDHEPDPGTWLMCSDGLSDLVPLEEMERIIAEA
                     GSDEGAVHGLWQAAMEASGRDNISILLARRV"
     misc_feature    complement(10369..10382)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 cgggtgcgat cgcctccgga tgcatggccg gattcagcat gaacacggcg gcaagggccg
       61 ccaccgccac ggcgacgccc catttcagca atggttccaa tgacttcgcc accggcctcc
      121 ccccagctca gggcctcagt ctctcctgtg cgaagggaaa ggtgacgcaa tggcaacggt
      181 atgtcgttac tctccgtttt tttactttcc gcagaggaaa gtggagttgg cggcgccatc
      241 ggcggccgtc aacggcaagg caggtgcaaa ggggaggacg ggccgcgggc gccgcagccg
      301 cggcgcgggc ctgccgcggc gcgcagcagg gcgcggcgcc ggggcggcga gggggcggga
      361 cgggcgcggc tccctgcggg gagggggcgt agggagccgc gccgggggcc ggccgggtca
      421 gccgtgttcg agcacggcct tgaggaacga cttcgtgcgc tcctcctggg ggtcgccgaa
      481 gatctgctcg ggcggaccct cctcggccac ctggcccgcg tcgaacatca gcacccggtg
      541 ggagacgtcg cgcgcgaagc tcatctcgtg cgtcacgcac agcatggtga tgtcggtcgt
      601 ctgcgcgatg ttgcgcagca cgtccagcac gcccgccacc agttcggggt ccagcgccga
      661 ggtgacctcg tcgagcagga ggatctgcgg ccgcatggcc agcgcgcggg cgatcgccac
      721 gcgctgctgc tgcccgccgg agagctgggt cgggtgggcg tcgatcttct cgttcagccc
      781 gaccagatcc agcagctcct cggcgcgctg gttggcctcg tccttggaga cgccgaggct
      841 gtgcacaggg ccttcggtga tgttcttgcg cacgctcatg ttcgggaaca ggttgaactg
      901 ctggaagacc atcccgatgt tcttgcgcac gctgcgcagg tgggcctcgt tggccttgac
      961 cagcttgtcg ccgcggcgca tgtgcgacag cggctccccg ttgacccaga tgacgccgcc
     1021 gtcgatgtcc tccagggtca tcagcaggcg caggatcgtg gtcttgccgg acccgctggg
     1081 cccgatgagc gtgacccgct cgcccggggc gaccgagaag tccagctcgt tgagcacgac
     1141 gttgctgccg tagcgcttga cgaccttgtc gaaccggatg atctcgccgc cgggtgcctc
     1201 ctgtgcggac tgcacgccgg cggtctccgt actgtgcgta ctctcagact gctgcataac
     1261 ggcgctccag acgtcggatg atgaccgcgg agggcacgct cacggccagg aagaacagcc
     1321 ctgccagggt gtaggcctcc atgctgcgga aggcctcggt gccgatcgcg ttggccacga
     1381 acagcatttc gcccacggtg atcgcgaaca ggtagggcgt gtccttgaac agggagatga
     1441 ggtagttgcc cagggccgga acgaccttgc ggatggcctg gggcagcacc acggcgcccc
     1501 acacccgcca gggcggcagg ctcagcgcgc ggcaggcctc ccactggccc ttggccacgc
     1561 cctcgatccc cgagcggtag acctcggcgg tgtaggccgc gtagtggacg cccagcaccg
     1621 cgacgccgat ggtcagcggc tccacggacg ccggagccag ggcgaacacg aacagcagct
     1681 gcaccagcac cggtgtggtc cggatgaact cgaagaccac gaagacgacc ggcccgatgt
     1741 agtcgatccg gcgcaggatg gccacgaaca gccccaggac gacggccacc aggtagccga
     1801 tcaccgtcgc ctggatggtg acggccaggc cttccagcag ccgggggatg atgctgatcg
     1861 cgaattcggt gtcccaggtc acttggccgc ccccactttc gttccgggtg tcggcgggcg
     1921 cagcaggtcc aggagcccgc cgccggccgg cgccctgccc agccgccggt tggcccggcg
     1981 ctccagcagc cgcatcccga agatcagcac ctgggcgatg acgaagtaca tgaccagggc
     2041 gctgccgaat gccagcaccg tctcacccga gctggagcgc tgctcctcgg cgacctgggt
     2101 gaggtcggcg accccgatga gcgacacgat cgccgacgcc ttcatcagct cgatgatcag
     2161 gttgccgaag gtcggcagca tctgcgccca cgcctgtggc agcaccacca gccacatccg
     2221 ccgcgcccac gacatgttca gcgcgatggt ggcctcggtc tgagccctgg gcaccgcgtt
     2281 gatcgccgcg cgcaccacct ccgcgccgta ggcgcccaca ttcagcccca gcgcgaggac
     2341 gccggcgaac atcggggaca gctggtaggg ggtgatctgc gggaccgcgt aggcgaacca
     2401 gaacagcagg atgagcgccg ccacaccgcg gaacgcctcg gtgtagaccg tggcgacggc
     2461 gcgggcgacc gggttgcgca gggtgccgag gatgccgatc accatggcca gcacgaaggc
     2521 caccgccgaa cccagcacgg tcatcagcag tgtgatcagg gccccgtcca ggtagagcgg
     2581 gaacctgttg aggaagaatt ccacgccggg gccttactgc gagcagagct cttcggtggt
     2641 gaggtcgccg gggtgggcgg cctcgtcgaa gccgaacggc ttgccgatct cggtcagccg
     2701 cccgctctcc ttgaagtcgg cgaggacctt gttgaactcc ttgaggaact cgtcgttctc
     2761 cttgcggaag gcgaacccgc cggcggggat gacctcctcg ccgtcgacga cgggggtgaa
     2821 cccctcggtc acctcgtagt cgtcctgcat gccgcgctgc tccatctggt agttcaggga
     2881 gaacgtgctc agccccacgg cgtccacgcg gccctgctcc agcaggtcga gcatctgcac
     2941 ggcgttgtcc cccagcgtca tctgctcctt ggtgacgccg ttgccctcgg cgtagttctg
     3001 ctcgacggag gcgttgagga ccgcgatctt gatgtcgggg ttctcggcga cggacttgta
     3061 gtcggagatg ttcttcgggt tgcccttggg caccatgaag gcctcgagca ccttgtagtc
     3121 gggctcggcg aaggcgacct cctggcagcg ctccggggtg atgtacatcc cggcggcgac
     3181 gacgtcgtag cggcccgcct tgagtccggg gatcaggccg tcccagctga cggtctcggc
     3241 ctcgatcttg tcgatgccca tctccttgac gatcgcccgc agcagctcgg gcgcctcgcc
     3301 ggtgaccttg ccgttgtcgt cgatgaagcc gaacgggggc tcgttgttga tcgccgcctt
     3361 gatggagccc ttctcacgca ggctctccag cagcgtctgc ccttcggcgg gctcggtgcg
     3421 ggagcacgcg gccagccctg gccccgcggc cagcgccgcg gcggtgacgc cgccgacgcg
     3481 gagcaggtcg cggcggccga ggtggctcag gggactgctg ttgctcattg gtttctcctc
     3541 gtcgcgaaac taagggggga ggcgaaaagc gcggcgcctc cccccgcgcg ggggcgcggg
     3601 aggaagcgcc gtgcggtggt acggacatgc gcggcccctg ggacggtccc cggtcgagca
     3661 gggatcggga cgtctcagta cccccggtca cgcgcggcgc ccggcaccaa ccggtgcctg
     3721 ccgactccgt gcccacgatc ccccagggct aacggcgtcg gaatagcacg ttatgcactg
     3781 gttatcccga tacttggccg ggaccggccc cgtaacgatc aggccacgga agccttagct
     3841 tcgcggtgtc gattcggtgt tgcggggtta tttcacgggg gtgaagtcct gggcgcccag
     3901 gaagtagggc cgcggcttgg atgcggcgaa gggcgcctcg caggtgttct cgacgctgtt
     3961 gtagacgatg aacacgttgg agcgcgggaa cggcgtgatg ttgccgttgg agccgtgcat
     4021 gcagttggag tcgaacatga cggccccgcc ggcgggcccg gtgagcacgt cgatcccgtg
     4081 ctggtcggcc aggatcgaca ggctgctctg gtcgggcgtg ccgatctcct gcttcttcag
     4141 cgacgcgcgg tagtggtcgt cgggcgtctc gccgacgcag gagacgaacg tcttgtgcga
     4201 gcccggcatg atcatcagcg agccgttgtg ctcgtagttg tcggtgagcg cgatggagat
     4261 gctcaccgcg cgcatccgcg gcatcccgtc ctcggcgtgc caggtctcga agtcggagtg
     4321 ccagaagaag tcggtgccgg agaacccggg cttgtagttc acccggctct ggtggatgta
     4381 gacgtcggag cccaggatct ggcgggcgcg gtcgaccagg cgggggtcgc gcaccagatc
     4441 ggcgaagacc gggctgatct tgtgcacctc gaagaccgag cgcacctcgt cactggcccg
     4501 ctccacgacg acgcgctcgt ccttcttcag ctcgtcgtcg gagctcagcc gcgtcagctc
     4561 ggcgcggaag gactccagct cgtcccgggt gatcagggat tcgaactgca ggtagccctt
     4621 ggcgtcgtag gagtcgaggg tggcggcgtc catcgggccg ctcgccgctt cgccccagac
     4681 ggtggggtcc ttgcggtaga gcagggccgg tgcctggtcc ttgcgggtcg ggtagtcgtc
     4741 catggtcggc tgttgaatgg tcaacgcagt catggccata ccccggaggg attcgctcct
     4801 tttcggtgtg cgcgcggcgt ccggtggccg cgccgtcgtt cgtcctcgct tctgtgcgga
     4861 ttttcgacct tttgccgtgc ggcgctcgcg cgccgcggcc cgcggcgctg ccgcgggttc
     4921 gcgcggggcg gggaccggcg cgccgggcgt gccggtccca agcgtgcggg gcgcggccgc
     4981 ggccgcgccc cgcacgggtc ctactgcggc ggggtgtcca gccgctcccc cgtctcggaa
     5041 agcagggggt acacaccgtt ctcgtcgtgc acctcgcggc cggtgacggg cgggttgaac
     5101 acgcacagca cccggaagtc cgtcttgggc cgcaccgtgt ggtgctcgtg gccgttgagg
     5161 aggtacagcg tgcccggagt gatgaggtgg gtctcgccgg tctcgtcgtt ggtgacctcg
     5221 gcctcgccct cgatgcagtg caccagttcg atgtggttgg cgtaccacat gctggtctcg
     5281 gtgcccgcgt agaggacggt ctcgtggaac gagaagccca ctccgtcctt ggccaggacg
     5341 acccggcggc tgcgccaggt ctcggtccgg acgtcggcgt cggtgccgtc gacgtcactc
     5401 agactgcgca cgatcaaggt gcctactcct cttgcttact cgtgtggccg aaggcggggt
     5461 cccgaacggg ccgtccggga ccccggtact ccttcactgc caattgtggg ggcgtctatg
     5521 ccccgcgccc agcgccggtc atgcgggctg cggagtcttc atcgcggcgc gcgcggcgtc
     5581 ggcgatgatc tccaggccct cctccagctc ctgctcggtc gtggtcagcg cgggcagcag
     5641 cttggcgacc tcgtcctcgg gcccggaggt ctccaggagc aggccgcgct cgaaggcctc
     5701 gcggctgagc gccttggccg cgcccgggcg ctccagcacc aggccgcggg ccataccccg
     5761 accgcgcacg tgggcctcgt cggggcccag ctcctcggcc acggcgcgca ggccgcggtc
     5821 gagcagctcg cccttggcct cggtgccctt ctgcagggcg tcatcgcgcc agaagagccg
     5881 catggcctcg gcgccggtga cgaagcccgg gttggggccg cggaaggtgc cgttgtgctc
     5941 gccgggctcc cacacgtcca gctcgcgctt gaacagcgtg agcgccatcg gcaggccgta
     6001 gccgctgatg gacttggaca gggtgacgat gtcgggctcg atgccggcgg tctcgaagct
     6061 gaagaacggg ccggtacggc cgcagcccat ctggatgtcg tcgacgatga gcagcatgtc
     6121 gtactcgtgg cagacctcct ggaggccccg cagccactcg gcgctggcga cgttgatgcc
     6181 gccctcgccc tgcacggtct cgacgatcac cgcggcgggc gcgtccagac cgctgccgct
     6241 gtcctcgagc aggctgcgca gccagaggaa gtcgggcgtc tggccgtcca tatagcggtc
     6301 gaagggcatg gtcgccgagt ggctcagcgg cacgcccgct ccaccgcgct tcatggagtt
     6361 gccggtgacc gcgagggcgc ccagcgtcat gccgtggaag ccgttggtga agctgatgac
     6421 cgtctcgcgg ccggtgtact tgcgggcgag cttgagggcg gcctcgacgg cgttgttgcc
     6481 tgcagggccg gggaactgga ccttgtagtc gagtccgcgc gggccgagga tcgtctcgcc
     6541 gaatgtcgcc aggaagtcgc gtttggcggt ggagtacgcg tcgaggctgt gcacgatcgc
     6601 gtcgctcatc aggtagtcga tgagtttgga tttcaactcc gggtgattgt gcccgtagtt
     6661 gagcgaacca gcacccgcga agaagtcgag gtagggtcgc cccgactcgt cgtagacgta
     6721 gctgccccgc gcctcgttga agacgaccgg ccagctacgg cagtagctgc ggacttcgga
     6781 ttcgaggcgt tcgaagatct ccatcgtgag ttccttgagt cctttcccca gtttcatggg
     6841 ctcacgtgtg tggcgcttgg ggttcgggtc ggtcaggatc gggtgtcagg tgtgtcggcg
     6901 gacgtgcttg tcgtcggtgc ccggtcgacc cagggggccg atccggacca ggtcttcggg
     6961 ttcgtgctcc tcgcttccgt cggctgggaa atgctcccgc ccgaacagcg gggtccacac
     7021 cgcttcgcat tccctggaac gggcgaagga ttcgaacagc gcgcgtgatg ccgcgttgtc
     7081 cggggtcacc gtggcctcca ggtggaccag cccgcgcgcg gcgatccgct cgccgatgtg
     7141 gtcgagcatc cggcgcgcca gccgccgccc gcggaatccg gggtcgacgc ccacctgcca
     7201 caggaagtag gtgtcggggc gtgtggggcg gatgaaaccg gtgacgaaac cgcgcggctg
     7261 cccggcttcg tcgcgggcga tgacgctggt gtcggcgaag tcccgggccc acagcgtgta
     7321 ggcgtagggc gagttgatgt cgagccctgt gtcgcgggcc agccgccaca ggtcggggcc
     7381 gtcggcaatg gtcggctcgg ccaggctgat gccgtcggaa gccgcccgcc cgccgactac
     7441 tgcgggctca tctccagcac cgggcacggt cctggtctcg tcgtcggtct gttggtcagg
     7501 cacgtcgaag aaacatagcg aacgttcctg agctgcgtgt cccgcgcgcc gacggcgaat
     7561 gtttcagccc gatgccaccg ggtatgaccc gcgggtttgt cacagcggtt actttgcgtc
     7621 gcagcgcggg agacgcgcgg gcgcctacgg agactgccct ctacgccatg ctcaccagga
     7681 ggttcggggc ggcgtccggg agcggcccga gagggcggcg gcgcctgcgg ccagcggaga
     7741 ttgttcctga taccaggcca acatagaggg actcgcagcc gattttaagg gtgtgttaag
     7801 tcacaccgat tcggatgaaa gctcgcgaca ttccaccccg attacggaca tcgaccccgt
     7861 gccccccgcc cccgcgtccc tcgggcgagt gccgcaaggc ccgccgccgc gggcgaaagc
     7921 ccacgaagaa cgggactccg cgcggtttcg gggtagcggg ccgcgggagc cgagcccgct
     7981 cgccggtgcg ggctcgaagg cggcgccgca ggtgcggcgg aacttgaagc acagcccgaa
     8041 attttggcag acttttcgta gtacgaccac acgtagtact acgatgggtc gcatcgcgat
     8101 ggagcggcgg ccaccgccct ccggctcgcg accagacacg tcccgcgtgc ggcaccggcc
     8161 gaccggcccc gcgcggggga ccggccaggg aacggaggct cctggtgaac cgcctttacc
     8221 acgacagcga agactgggtc gcgcagggcg catgccggca gtacgacccg gaactcttct
     8281 tccccgtcag cacgacgggc cccgggcaga acgacgcgga gctcgccaag gccatctgcc
     8341 gcacctgcgc ggtgcgctca gagtgcctga gctgggcgct gcgcgcgggc gagccgcacg
     8401 gcgtctgggg cgggaccacg ccggaggacc gccgttacat gcggcgccag ccgctgccga
     8461 tggcctccta gcctcctgcc cttccgccga ccggattccc ggggcaccgg tccgccgagg
     8521 cggcgccgga cgcaccggtg cgcgcggtcc ctcaggcggc gtcggctagg ttgaggagtc
     8581 gtgcggcgtc cacgcgcccg gctccgcgaa ccgcctccgc gccgagggtt ggggcgcgcg
     8641 ggcgccgcag ggcccgcggc agcagccgtg taccgggccg cgcggccgac cggccgtgtg
     8701 cgcgccggtt ccagcccgcc caccggacac gctcacccct cgaaggaccc gccgatgaac
     8761 ctggccgacc ccgcagccgc gtccccagca cagcggcgcc gacgcgcggc gcggcgcccg
     8821 ggcgtcgccg ccgccgcgct gctgggctcc ctggcgctcg ccaccgcgac ggctcccgcc
     8881 gcgctcgccc acgacaccct catcgactcc tcgccccagg acggcgccga actggacacc
     8941 gcgccggacg aggtggtgct gaccttcagc aacgacatcg gccagggcgg caacgcgatc
     9001 gtggtcaccg gtcccgaggg ggagacccac gaggagggcc cggtcgagat cgacggcccc
     9061 gaagccgccg tcggcctgaa ggaactgccg gccgccggcg aatacacggt cgcctaccgg
     9121 gtggtctcct ccgacggcca cacgctggag gacgagatcg ccttctccgt caccgaggcg
     9181 gccgtgcccg aacccagccc ctcccccgaa gccgagtcgc cctcggcccc gccgaccggc
     9241 ggcgacgaga ccggcggcgc cgacgccgag gaggctcccg cggcccagtc gccgaccgac
     9301 cccatgtcgt ccctgctcgg gccggtcggc ggcgtgatcg cggcgatcgc cgtcctcgcg
     9361 ctgatcgcca tcctgctcat ccgcatacgc aaccgccccg gcggccgcga cggcgcggac
     9421 ggcgacgggg gcggcgacag ctagcgcccc gaccgcaggc gggtgccggg gggcgggcgg
     9481 cgcccgcccc cggcacccct cagacgcggc gggccagcag aatgctgatg ttgtcgcggc
     9541 cggaggcctc catggccgcc tgccacaggc cgtgcacggc gccttcgtcg ctgcccgcct
     9601 cggcgatgat gcgctccatc tcctccagcg gtaccaggtc ggagaggccg tcgctgcaca
     9661 tcagccaggt gccggggtcg ggctcgtggt cgcgtccgac gtggggcacc atcgccgtgc
     9721 tgccgctgcc gcccagcgac tgggtgatga agttcgtggt gaccggcttg ccgtcgtcgg
     9781 aggggggcag cggcggcgag tcgtcctggg agagctgccg caaccgggtg ccctccagcc
     9841 ggtaggtgcg ggagtcgccg acgttgaacc agtagttgcc gtcggtgttg agcagcaggc
     9901 ccgccacggt ggtgcccatc ccggagaact ccgtgtgctg ggtggcgtgg tccttgatct
     9961 cctcgtcgat gttcttgagc aggacgtcga cgtcgtcggg gccggccagc cgcgggctca
    10021 tctcggccat gcggtggacg gcgtgctcgg aggcgatctc gccggccgcg tgcccgccca
    10081 gcccgtcggc gaccgcgagg atcaccggct cgctgaccgg cagtacgcac ctcaccggca
    10141 aggtcatgtt ggcgctggcg atggtgagcg cacccatgac cacggcgtcc tcgttggccg
    10201 agcgtacggc gccccggtgt gtgagggctg tgaccgtgac ttccccacca gcgacaccca
    10261 tacgcccaac ctcctgtttc aacggcagta cctgccgaac agtccaagcg gcgcctggcc
    10321 gttcgggagg ccgcggcgcg ttgatcatgc aaccgtatcg gggcaccgct gatcgtggtc
    10381 aaaatccgcg tacatcatgg cggaaatcgc cctcata
//
