LOCUS       NZ_VTZW01000016        11431 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomonospora sp. PA3 scaffold3.1, whole genome shotgun sequence.
ACCESSION   NZ_VTZW01000016
VERSION     NZ_VTZW01000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomonospora sp. PA3
  ORGANISM  Streptomonospora sp. PA3
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Streptomonospora.
REFERENCE   1  (bases 1 to 590251)
  AUTHORS   Matroodi,S., Siitonen,V., Baral,B., Yamada,K., Akhgari,A. and
            Metsa-Ketela,M.
  TITLE     Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated
            Halophilic Streptomonospora sp. PA3
  JOURNAL   Front Microbiol 11, 1237 (2020)
   PUBMED   32582127
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590251)
  AUTHORS   Matroodi,S., Siitonen,V., Baral,B., Yamada,K., Akhgari,A. and
            Metsae-Ketelae,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2019) Department of Biochemistry, University of
            Turku, Turun Yliopisto, Turku 20014, Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2018
            Assembly Method                   :: a5-MiSeq v. MAY-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_000695235.1
            Genome Coverage                   :: 292.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009728995.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 10:59:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,000
            CDSs (total)                      :: 4,917
            Genes (coding)                    :: 4,877
            CDSs (with protein)               :: 4,877
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 8, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 7, 10 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 8 of 40
            Pseudo Genes (incomplete)         :: 35 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            Pseudo Genes (multiple problems)  :: 3 of 40
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:29:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 160536
            Orig. end                         :: 171967
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VTZW01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11431
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11431
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11431
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6431](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6431
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(56..289)
                     /locus_tag="FZ103_RS17215"
                     /old_locus_tag="FZ103_17200"
     CDS             complement(56..289)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FZ103_RS17215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FZ103_17200"
                     /product="hypothetical protein"
                     /protein_id="WP_156002518.1"
                     /transl_table=11
                     /translation="MERSTVVDAERIFLIVLLVVFAALFTAVVIKPGIVPRRTSLRGNP
                     RTRAFVHGSTRFTAVCLLLLVAAALFHPDTWS"
     gene            566..2104
                     /locus_tag="FZ103_RS17220"
                     /old_locus_tag="FZ103_17205"
     CDS             566..2104
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615138.1"
                     /locus_tag="FZ103_RS17220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17205"
                     /product="sodium:solute symporter family protein"
                     /protein_id="WP_156002519.1"
                     /transl_table=11
                     /translation="MNDGSPVYLIAFIGALVVMLGIGVWVARRTSSGEDFLLAGRKLGT
                     PLLMGTTLATLVGTGSSLGAVGFAYENGWAGALYGIGGAAGVIGLIWLFSDVRRHGFMT
                     FAEELSFYFGANRLIKGVVSIIMLLASVGWLGAHIMGGALYLSYLTGMEPTLAKIVVAL
                     GFGLYTVIGGYLAVVVTDALQGTILFVGFTVLAAVAFVQAGGFSGIEQGASAEATSMLG
                     IEGLGVLPAISLALVIAVGVLATPSYRQRIYSAADVGTVRRSFGLAALLFAVFAVLPAV
                     AGLAAHAIEPGLENPDTAFPMLATTVFPVWLGAFLLIAGLSATMSSGDSDAVAGVTILL
                     RDVVQLVTGRLPQAEHMVRYSRIALVCVLALALAGALIADTIIEYITMMISTVLTGLLV
                     ASLLGKFWRRSTWQGGLAAMIGGSATSLAVQNIAAWTEFWGNPVIPSLVVALAAGVLVS
                     LVTPRSTVTREEALRLLAEERAALDVGTHLRAADTAGADAADGGAETEPPGPRT"
     gene            complement(2312..3280)
                     /locus_tag="FZ103_RS17225"
                     /old_locus_tag="FZ103_17210"
     CDS             complement(2312..3280)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604401.1"
                     /locus_tag="FZ103_RS17225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17210"
                     /product="FkbM family methyltransferase"
                     /protein_id="WP_156002520.1"
                     /transl_table=11
                     /translation="MPTLKRRILDALPRANIHVRNLEPGTALLSRRDAYRVSRIGPTSW
                     LVSRNRDPFAKALSRAIGKSARRPRRVVPFGPDSHLLVADDQDAEGEWHMHKAAHDYLA
                     ERHVASLLRDYRVNCVFDVGANVGQYARRLRKHGYTGRIVSFEPVSAIAEKLREAAADD
                     PDWWVYPCALGREERVDTINVVQGSMSSLLGPSEFGTRRYKRFRKTAAEEIAVRRLDAV
                     MDEALKGIDDPRPYLKLDTQGYDLEAFAGAGARTADLVGLQSEVALMQIYEGMPRMQEA
                     ITVYEDDGFEITGMFPVTREQATGRVLEFDCVMVRADSLPE"
     misc_feature    complement(3303..3317)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.94"
                     /tool="antismash"
     gene            complement(3410..3643)
                     /locus_tag="FZ103_RS17230"
                     /old_locus_tag="FZ103_17215"
     CDS             complement(3410..3643)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FZ103_RS17230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FZ103_17215"
                     /product="hypothetical protein"
                     /protein_id="WP_156002521.1"
                     /transl_table=11
                     /translation="MDALTNELTDPMNLLLLPFVAGLTAVVVKPKLVLHRTHRRAVERM
                     PRMGPIVLWIYRVTAAVMLVVLLWRVLWPDTA"
     gene            complement(3782..4723)
                     /locus_tag="FZ103_RS17235"
                     /old_locus_tag="FZ103_17220"
     CDS             complement(3782..4723)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF025217.5"
                     /locus_tag="FZ103_RS17235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17220"
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="WP_156002522.1"
                     /sec_met_domain="PCMT (E-value: 7.8e-06, bitscore: 20.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSTDADTAGRVDDLLRGAWVLAALARAADTGDPLDPELGAALAA
                     AGFAEQAEGAWRLLPEYRALSRGRGAGGAGLSGRIGRMLRWAADAAEGLPAPTGSHSDA
                     ELREEGDESGRRFAALLDALAGADSGLRELLASPGLRFLDIGTGTAAIAATVAVRVPGS
                     SAVGLDRDPHVLGLAAERIASGGLGGRVRVQCADAAGIAGSAEYDLAWLPLAVLEPEAA
                     LEALPRLRAALRPGGTLIAATVLQDGTAAQRDPLGQALARWRMARAGITTWTPEEAAQR
                     LRDAGFTGVRRPEAADQPVAAVLARAPEHTPP"
     misc_feature    4816..4828
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    4868..4882
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            5001..6431
                     /locus_tag="FZ103_RS17240"
                     /old_locus_tag="FZ103_17225"
     CDS             5001..6431
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589866.1"
                     /locus_tag="FZ103_RS17240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17225"
                     /product="YcaO-like family protein"
                     /protein_id="WP_255430086.1"
                     /sec_met_domain="YcaO (E-value: 4.4e-38, bitscore: 126.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRSEWIAAPVRPVGSRPGGRGEPGPEWAGAGERELPLAGAERQA
                     LADIAALGWRARHRILDADQPTAAQCELRYGDGSEVPYGLGAGKGPLAAARVGAHFEAL
                     EHALSGPVSLDGLAVACRRAAELTGGPFRSDRAVSGLAARPDARVACLDYEPLDGGAPF
                     AAPLYLWAPWYPVSTAAMARRRAALGDTAAYRPLVAYTVNTGCAIGATAEEALLHALNE
                     WAERDAFSLFLLCSVYDRGPMPGRIDPAALPDSSAGLLGRARDLVGADVALLDLTTDLG
                     IPVVMAYCPGLGGGSARYYALGASLSGRIAVERALTEFVQGELLARVVADRAARGTPAQ
                     PRRGEHPSFDEVVAEHDTASAVRERLSGHPRLLACAQLDFADRVAEAAPVGPPGEAVPE
                     GTGVAAQRAAAVERIAAAGHRVGFHTLRRLELGTTLVQVQCPGLERFHQVTNGHVALPG
                     TRGRAHRRRGRPTGAAAD"
     gene            complement(6537..7370)
                     /locus_tag="FZ103_RS17245"
                     /old_locus_tag="FZ103_17230"
     CDS             complement(6537..7370)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594472.1"
                     /locus_tag="FZ103_RS17245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17230"
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WP_156002523.1"
                     /transl_table=11
                     /translation="MLAAQRQALIIEQVRRSGGVRVTELVESLDVSDMTVRRDLDTLEA
                     RGLVRKVHGGAVAPHRHSTEEPGFEANSTRQEAEKQAIARAAARLVEPDSAIGLSAGTT
                     TCAVAHHLCDVPGLTVVTNCLRVADVFYRSARSDHTVVLTGGFRTPSDALVGPLAVAAV
                     RSLHLDTLLLGVHGMDDRAGLTTPNLMEGETDQALVEAARKLVVVADHTKWATVGLSTI
                     APLERCDVLVTDSGLGEEARRLLSERVGGLVIADEPAGDGPAPGGAGPVPPSRRG"
     misc_feature    7461..7475
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.19"
                     /tool="antismash"
     misc_feature    complement(7668..7682)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.97"
                     /tool="antismash"
     misc_feature    complement(7669..7682)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.01"
                     /tool="antismash"
     misc_feature    7965..7979
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.46"
                     /tool="antismash"
     misc_feature    complement(7988..8002)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     gene            7996..9678
                     /locus_tag="FZ103_RS17250"
                     /old_locus_tag="FZ103_17235"
     CDS             7996..9678
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610234.1"
                     /locus_tag="FZ103_RS17250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17235"
                     /product="sodium:solute symporter family protein"
                     /protein_id="WP_186356442.1"
                     /transl_table=11
                     /translation="MIALAQEQLRLDANALDYGLLALYFVFVLGIGFLAKRSVSTSLDF
                     FLSGRSLPAWVTGLAFIAANLGAIEIIGMSANGARYGMATMHYFWVGAVPAMLFLGLVM
                     MPFYYGSKVRSVPEFMNRRFGKAAHLVNGISFAVAQILIAGINLFLLATIVDALLGWPL
                     WLSLVVAAAIVLSYITLGGLSAAIYNEVLQFFVIVAALLPLTLAGLNRVGGWSGLVDEL
                     SQTDLGAEQASTWPGNALTGFESDILSVFGIVFGLGFVLAFGYWTTNFVEVQRAMASKS
                     MSAAMRTPIIGAFPKLLIPFIVVIPGMIAGVTASEMVALKAGENTNVTYNDAILLLMRD
                     LLPNGLLGIALAGLLASFMAGMAANLSSFNTVFTYDIWQSYIVKDRPDSYYLNMGRWVT
                     MGATVAAVGTAYIASGYSNLMDYLQQLFSFFNAPLFATFILGMYWKRMTPHAGWSGLVC
                     GTAAAIVVFVLGETGVLGLSAQGASFVGAGAAFVVDIIVSVVVTMFTRPKPDSELVGLV
                     HSLTPREQRRPSHSGEDAGWYRRPALLAAIVAVITIALNIIFA"
     gene            9728..10003
                     /locus_tag="FZ103_RS17255"
                     /old_locus_tag="FZ103_17240"
     CDS             9728..10003
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610235.1"
                     /locus_tag="FZ103_RS17255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FZ103_17240"
                     /product="hypothetical protein"
                     /protein_id="WP_156002525.1"
                     /transl_table=11
                     /translation="MSADSPGGGGRQRAKAFDVRTIIALLFVIYGAVLVVMGFVQPADE
                     ARQAGTNLNLWSGLGMLAFSAAMGGWVLLKPLKVPENQEGGQGPQS"
     misc_feature    10254..10274
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.81"
                     /tool="antismash"
     misc_feature    10368..10382
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.33"
                     /tool="antismash"
ORIGIN
        1 gccccgcctt tgcagcccgc ggggcggcgg cggggcggac gggggcctcg gcgcgctagc
       61 tccaggtgtc ggggtggaac agtgcggcgg cgaccagcag aagcaggcac acggccgtga
      121 agcgggtgct gccgtgcacg aacgcccgcg ttcgggggtt cccgcgcagg ctcgtccgtc
      181 tcggcacgat cccgggcttg atcaccaccg cggtgaacag cgctgcgaag accaccagca
      241 gcacgatcag gaatatccgc tcggcgtcca cgacggtgct ccgttccacg tcggctcccc
      301 gtaaccgcgg gctgcggcgc ggggcctcgc accgccagac gcacccgccg gggccccggt
      361 tccctcggag ccgatccgcg gcggcacccg ccgcttgcac cccgatcccg ccgtcccacc
      421 gacgcaccac cgtccccctg cgatgcggga cgccctaccg ggccttcccg gtgcggtctt
      481 gcaccgcagc acgccgcgtc cgcgcggctt ccctgacccc cttccccggc cgccgcggcc
      541 gggcttccgg caacggagac agaccatgaa cgacggcagt cccgtatacc tcatcgcctt
      601 catcggcgcc ctcgtcgtca tgctgggcat cggcgtgtgg gtcgcccgcc gcaccagcag
      661 cggcgaggac ttcctgctgg ccgggcgcaa gctcggcact cccctcctga tgggcacgac
      721 cctggccacc ctggtcggca ccggctccag cctcggcgcg gtgggcttcg cctacgagaa
      781 cggctgggcc ggcgccctct acggcatcgg cggcgccgcc ggggtcatcg gcctgatctg
      841 gctcttctcc gatgtgcgcc ggcacggctt catgaccttc gccgaggagc tgagcttcta
      901 cttcggcgcc aaccgcctga tcaagggcgt ggtgtcgatc atcatgctgc tggcgtcggt
      961 ggggtggctc ggcgcgcaca tcatgggcgg ggcgctctac ctgtcctacc tcaccggcat
     1021 ggagcccacc ctcgccaaga tcgtcgtcgc gctcggcttc gggctctaca ccgtcatcgg
     1081 cggctacctc gccgtggtgg tgaccgacgc gctgcagggc acgatcctgt tcgtcggctt
     1141 cacggtgctg gccgcggtcg ccttcgtgca ggccggcggg ttcagcggaa tcgaacaggg
     1201 cgcgtccgcg gaggcgacct ccatgctggg gatcgagggc ctgggtgtgc tgcccgccat
     1261 ctcgctggcg ctggtcatcg cggtcggggt gctcgccacc ccgtcctacc gccagcgcat
     1321 ctactcggcg gccgacgtgg gcactgtgcg gcgcagcttc gggctggccg cgctgctgtt
     1381 cgccgtcttc gccgtgctgc cggccgtcgc gggactggcg gcgcacgcca tcgagcccgg
     1441 cctggagaac ccggacaccg ccttccccat gctggcgacc acggtgttcc ccgtgtggct
     1501 gggcgcgttc ctgctgatcg cgggcctgtc tgcgacgatg tcctccggcg actccgacgc
     1561 ggtcgcgggg gtgacgatcc tgctgcgcga cgtggtgcag ctggtcaccg gccggctgcc
     1621 gcaggccgag cacatggtgc gctactcgcg gatcgcgctg gtgtgcgtgc tcgctctggc
     1681 gctggcgggg gcgctgatcg ccgacaccat catcgagtac atcaccatga tgatctcgac
     1741 ggtgctcacc ggcctgctgg tggcctcgct gctgggcaag ttctggcggc ggtcgacctg
     1801 gcagggcggg ctggccgcca tgatcggcgg ttcggcgacg tcgctggcgg tgcagaacat
     1861 cgcggcctgg acggagttct ggggcaaccc ggtgatcccg tcgctggtgg tggcgctggc
     1921 ggccggggtg ctggtcagcc tggtcacacc gcggtccacc gtcacccgcg aggaggcgct
     1981 gcgcctgctg gccgaggagc gcgccgccct ggacgtgggc acgcacctgc gcgcggccga
     2041 caccgccggc gccgacgcgg ctgacggcgg cgccgagacg gagccgccgg ggccgcggac
     2101 ctagccggtc cgcggttgag aacggcgggg gcgccgcttc ccgggaaccc gccgggtacc
     2161 gccttcttga acacgacgta gccgcccggc gcacgccgct gcagcgccct cgccggtctc
     2221 gcaggcgggg gcgtgagcgc ggccgcggcg tcgggggcgt cggctgggga tggagtgccg
     2281 cgtccggcgg cgggggccgc caccgctcga actattcggg cagcgagtcc gcgcgcacca
     2341 tcacgcagtc gaactccagc acgcgcccgg tggcctgctc gcgggtcacc gggaacatgc
     2401 cggtgatctc gaagccgtcg tcctcgtaga cggtgatcgc ctcctgcatt cggggcatgc
     2461 cctcgtagat ctgcatgagg gcgacctcgg actgcaggcc cacgagatcg gcggtgcgcg
     2521 cgcccgcgcc ggcgaaggct tcgaggtcgt agccctgggt gtcgagcttg aggtaggggc
     2581 ggggatcgtc gatgcccttg agcgcctcgt ccatgaccgc gtcgagccgg cgcacggcga
     2641 tctcctcggc ggcggtcttg cggaaccgct tgtagcgccg ggtgccgaac tcgctgggcc
     2701 ctagcagcga actcatcgat ccctgcacca cgttgatggt gtcgacgcgc tcctcgcggc
     2761 ccagcgcgca ggggtagacc caccagtcgg gatcgtcggc ggcggcttcg cggagcttct
     2821 cggcgatggc ggagaccggc tcgaaggaga cgatccgccc ggtgtagccg tgcttgcgca
     2881 gccggcgcgc gtactgcccg acgttggccc ccacgtcgaa gacgcagttg acccggtagt
     2941 cccgcagcag cgacgccaca tgccgttcgg ccaggtagtc gtgtgcggcc ttgtgcatgt
     3001 gccattcgcc ctcggcgtcc tggtcgtcgg cgaccagcag gtgggagtcg ggcccgaagg
     3061 gcaccacgcg ccgcggcctg cgggcggact tgccgatcgc ccgggacagc gctttggcga
     3121 aggggtctct gttgcggctg accagccagg aggtgggacc gatgcgggag acgcggtagg
     3181 cgtcgcggcg cgacagcagc gcggttcccg gttcgaggtt gcgcacgtgg atgttggccc
     3241 tggggagagc gtcgagaatg cgtcgcttca gcgttggcat gcttcccagc gtactctcag
     3301 cgtattctca ggaaataaaa aagctcggga aatccgctgg tcccgctgga gcaccgcacg
     3361 cacaccccgg tccggcggcg gggcgcttcg ccgcggtcgg gcgccatgct cacgcggtgt
     3421 ccggccacag cacccgccac aggaggacca cgagcatcac cgccgccgtg acgcggtaga
     3481 tccacagcac gatcgggccc atgcgcggca tccgctccac cgcgcggcgg tgcgtccggt
     3541 gcagcaccag cttcggcttg accaccactg cggtcagacc ggcgacgaac ggcagcaaca
     3601 gcaggttcat ggggtcggtg agctcgttgg tgagcgcgtc catcacgggt tctccgttcg
     3661 tgtcagatgt atcggggcgt gacacgagcg ttctgccgga catagacgcg tgcgccccgc
     3721 cgccggttcc gccggggccg ccgacatccg cgcgccggcg ggcgcgccgc tgccgccggg
     3781 ctcacggcgg cgtgtgctcg ggggcgcggg ccagtaccgc ggccaccggc tgatcggcgg
     3841 cctcggggcg gcgcacaccg gtgaaccccg catcgcgcaa ccgctgcgcc gcctcctcgg
     3901 gcgtccaggt ggtgattccg gcacgggcca tgcgccagcg cgccagcgcc tggccgagag
     3961 ggtcgcgctg cgcggcggtg ccgtcctgga gcacggtcgc ggcgatgagc gtgccgccgg
     4021 ggcgcagcgc cgcgcgcaac cgcggcagcg cctccagcgc ggcctccggt tcgagcaccg
     4081 ccagcggcag ccacgccagg tcgtactccg cggagccggc gatcccggcg gcgtccgcgc
     4141 actgcacccg gacccgtccg cccagaccgc cgctggcgat gcgctcggcg gcgaggccga
     4201 ggacgtgcgg gtcgcggtcc agaccgaccg cgctggagcc ggggacgcgc acggccaccg
     4261 tcgcggcgat cgcggcggtg ccggtgccga tgtcgagaaa acgcagtccc ggcgaggcga
     4321 gcagctctcg cagaccggag tcggcaccgg ccagggcgtc cagcagcgcg gcgaagcgcc
     4381 ggccggactc gtcgccctcc tcgcgcagtt cggcgtcgct gtgggatccg gtcggcgcgg
     4441 gcagcccttc ggccgcgtcg gcggcccagc gcagcatgcg gccgatccgg ccggacagcc
     4501 cggccccgcc ggcgccgcgc ccccggctca gcgcccggta ctccgggagc agccgccagg
     4561 cgccctcggc ctgctcggcg aatccggcgg cggccagggc cgcgcccagt tcggggtcga
     4621 gcgggtcgcc ggtgtctgcg gcgcgggcga gcgccgccag cacccacgca ccgcggagca
     4681 ggtcgtcgac gcggccggcg gtatcggcgt cggtggagga catgccggga gtctgccatg
     4741 cccgctaccg ctcggccgcc tgatcccggg gccggccgac gggtgcggca aagatcgccg
     4801 gatccggcca aaggggtgac actgcgaccc ggttcagcta caaaaggttg acattgaggc
     4861 tgaatcggtc tcaatcgccg aacactctgt ggagtcgaac atggcctgtg ttgcttacga
     4921 gtggaacgtc accctcgacc acgccgacga cctcgacgac gccgtacggg agctcgacga
     4981 ggccgcggaa gagtagtccc ttgccgcggt ccgaatggat cgccgcgccc gtccggccgg
     5041 tgggcagccg accgggcggg cgcggggagc ccggcccgga gtgggccgga gccggcgagc
     5101 gcgagctgcc gctggccggc gccgagcggc aggccctcgc cgacatcgcg gccctgggct
     5161 ggcgggcgcg ccaccgtatc ctggacgccg atcagcccac cgcggcccag tgcgagctgc
     5221 gctacggcga cggctccgag gtcccctacg gtctgggcgc cggcaagggc cccttggccg
     5281 cggcgcgggt gggcgcgcac ttcgaggccc tggagcacgc gctgagcggc ccggtgtccc
     5341 tcgacgggct ggccgtcgcg tgccgccgcg ccgccgagct gaccggcggg ccgttccggt
     5401 cggaccgcgc ggtcagcggc ctggccgccc ggcccgacgc gcgcgtggcc tgcctggact
     5461 acgagccgct ggacggcggg gcgccgttcg ccgccccgct gtacctgtgg gcgccctggt
     5521 atccggtctc cacagcggcc atggcgcggc ggcgcgcggc gctgggcgac accgccgcct
     5581 accgcccgct ggtcgcctac accgtcaaca ccggctgcgc catcggcgcc acggccgagg
     5641 aggcgctgct gcacgcgctc aacgagtggg ccgagcgcga cgccttctcc ctgttcctgc
     5701 tgtgctcggt ctacgaccgc ggacccatgc cggggcgcat cgaccccgca gcgctgccgg
     5761 actcctcggc gggcctgctg ggacgcgccc gcgacctggt gggcgccgac gtcgcgctgc
     5821 tggacctcac caccgacctg ggcatccccg tggtcatggc ctactgcccc ggcctgggcg
     5881 gcggttccgc gcgctactac gccttgggcg cctcgctgtc gggccggatc gccgtcgaac
     5941 gggcgctcac cgagttcgtc cagggcgagc tgctggcccg ggtggtcgcc gaccgggcgg
     6001 cgcgcggcac ccctgcgcag ccgcggcggg gcgagcaccc ctccttcgac gaggtggtgg
     6061 ccgagcacga caccgcatcg gcggtgcgcg agcggctgag cgggcacccg cggctgctcg
     6121 cctgcgccca gctggacttc gccgaccgcg tcgccgaggc cgcgccggtc gggccgccgg
     6181 gcgaggcggt gcccgagggc accggcgtgg ccgcacagcg cgcggcggcg gtggagcgga
     6241 tcgcggccgc cggccaccgc gtcggcttcc acacgctgcg ccgcctggaa ctgggcacca
     6301 ccctggtaca ggtgcagtgc cccgggctgg agcgcttcca ccaggtgacc aacggtcatg
     6361 tggcactgcc ggggacgcgg ggccgcgccc accgccgccg cggtcggccg accggcgccg
     6421 ccgcggactg agccctcggc tccgcgcggc ccccgcccgg ggcggtctgc ggctccgacc
     6481 gcggcgagcc gcgcggacgg cgacccggcg aggttctcct ccccggcgga ggagccttac
     6541 ccccggcggc tcggcgggac cggccccgcc ccgcccggcg ccgggccgtc gccggcgggc
     6601 tcgtcggcga tgaccagtcc gcccacccgc tcggagagca gccggcgggc ctcctcgccc
     6661 aagccggagt cggtgacgag cacgtcgcag cgctccagcg gcgcgatggt gctcaggccg
     6721 accgtcgccc acttggtgtg gtcggccacg accaccagct tgcgtgcggc ctccaccagc
     6781 gcctggtcgg tctcgccctc catgaggttg ggggtggtca gcccggcccg gtcgtccatg
     6841 ccgtgcacgc ccagcaggag cgtgtccagg tgcaggctgc gcaccgcggc gacggccagc
     6901 gggcccacga gcgcgtcgga gggggtgcgg aacccgccgg tcagcacgac ggtgtggtcg
     6961 gagcgcgcgg agcggtagaa gacgtcggcc acgcgcaggc agttggtgac gaccgtcagg
     7021 ccggggacgt cgcacaggtg gtgggcgacc gcgcaggtgg tggtgcccgc ggacaggccg
     7081 atggcgctgt cgggctcgac cagccgggcg gcggcgcggg cgatggcctg cttctcggcc
     7141 tcctggcggg tggagttggc ctcgaatccg ggttcctcgg tgctgtgccg gtggggggcc
     7201 acggcgccgc cgtggacctt gcgcaccagg ccgcgcgcct ccagcgtgtc gaggtcgcgc
     7261 cgcaccgtca tgtcggagac gtcgaggctc tcgacgagct cggtgacgcg gactccgccg
     7321 gatctccgca cctgttcgat gatcagtgcc tgtcgctgcg cggccagcat gcgtcccccg
     7381 tcgcgagcgg gtcgcacggc ccgccggcgc gggtccggac cgcccgcggc gcaccgcgga
     7441 gccgcacgac cgtgttggaa agtgttcgga taatagcgac ataccgcaca tccgtgtcaa
     7501 gacaccgacc ggccagtcgg accgttcgcc cttgctcccg ccctgctggg gatgcgtgtg
     7561 cccaggaccg caggggcgtg tcaaatcggg gttgcggaac ggcccgtggg acgcttgaca
     7621 cacccggggc cgcgcaggag agtcgcaccg aatgtgcgtt gatgttcgat ttcgtgagaa
     7681 attgacgcag taccggtagt accccgcacg acaaaacgca gagggatcgg ggtaggggcc
     7741 cggtcgtcca tccgtcccgc tccaccgcag cggcgaggcg ggcgccggcc gcgacaccgc
     7801 gccccgcccg cgcccgcggc cgccggtggc tcgcgggcac cgcccccgat gccgtgcagc
     7861 gcaccgcccg cggtgccgga cgggggcgcc gggcgccgaa gcgccgcccg gcgccccggg
     7921 ccggtccgcg gccggtgcgc tcgcggcagc aacccccatt gggaggacca tcgtgttcag
     7981 agaggactgg ccgaagtgat cgccctagca caagagcaac tgcggctgga cgccaatgcc
     8041 ctcgactacg gcctgctcgc gctgtacttc gtgttcgtgc tgggcatcgg cttcctcgcc
     8101 aagcgctcgg tctcgaccag ccttgacttc ttcctgtccg ggcggtccct gccggcctgg
     8161 gtgacgggtc tggccttcat cgcggccaac ctgggcgcca tcgagatcat cggcatgtcg
     8221 gccaacggcg cccgctacgg catggcgacc atgcactact tctgggtcgg cgccgtaccg
     8281 gcgatgctgt tcctgggcct ggtgatgatg ccgttctact acggctccaa ggtccgcagc
     8341 gtccccgagt tcatgaaccg ccgcttcggc aaggcggccc acctggtcaa cggcatcagc
     8401 ttcgccgtcg cccagatcct gatcgccggc atcaacctgt tcctgctcgc caccatcgtc
     8461 gacgcgctgc tgggctggcc gctgtggctg tcgctggtgg tcgccgccgc catcgtgctc
     8521 agctacatca cgctgggcgg gctctccgcg gccatctaca acgaggtgct gcagttcttc
     8581 gtcatcgtgg ccgcgctgct gcccctgacc ctggccgggc tgaaccgcgt cggcggctgg
     8641 tcgggcctgg tcgacgagct ctcccagacg gacctgggcg cggagcaggc ctcgacgtgg
     8701 ccgggcaacg cgctcaccgg cttcgagagc gacatcctca gcgtgttcgg catcgtcttc
     8761 ggcctgggtt tcgtcctggc cttcggctac tggacgacca acttcgtcga ggtgcagcgc
     8821 gccatggcgt ccaagagcat gtcggcggcc atgcgcaccc ccatcatcgg cgccttcccg
     8881 aagctgttga tccccttcat cgtcgtcatc cccggcatga tcgcgggtgt gaccgcctcg
     8941 gagatggtcg cgctcaaggc cggggagaac accaacgtca cctacaacga cgccatcctg
     9001 ctgctcatgc gcgacctgct gcccaacggc ctgctcggca tcgcgctcgc cgggctgctg
     9061 gcgtcgttca tggcgggcat ggcggccaac ctcagctcgt tcaacacggt gttcacctac
     9121 gacatctggc agtcctacat cgtgaaggac cggcccgaca gctactacct gaacatgggc
     9181 cgctgggtga ccatgggcgc caccgtggcg gccgtgggca ccgcctacat cgcctcgggc
     9241 tactccaacc tgatggacta cctgcagcag ctgttctcgt tcttcaacgc accgctgttc
     9301 gcgacgttca tcctcggcat gtactggaag cggatgaccc cgcacgcggg ctggagcggc
     9361 ctggtctgcg gcaccgccgc ggcgatcgtg gtgttcgtcc tgggcgagac cggcgtgctc
     9421 ggcctgtcgg cccagggagc gagcttcgtc ggcgccggcg cggccttcgt ggtggacatc
     9481 atcgtcagcg tcgtggtcac catgttcacc cggcccaagc ccgactccga gctcgtggga
     9541 ctggtgcact cgctgacccc gcgcgagcag cgccgcccct cgcacagcgg cgaggacgcg
     9601 ggctggtacc gccgtccggc gctgctcgcg gccatcgtcg cggtgatcac catcgccctg
     9661 aacatcatct tcgcctgacc ggacgaaggg cagagatgcg cagaaacgct gccaaggagg
     9721 caatccgatg agtgctgaca gccccggagg gggagggcgc caacgcgcca aggccttcga
     9781 cgtccggacg atcatcgcgc tgctgttcgt catctacggc gcggtcctgg tcgtgatggg
     9841 cttcgtgcag cccgccgacg aggccaggca ggcgggcacc aacctgaacc tgtggtcggg
     9901 cctgggcatg ctcgccttca gcgcggcgat gggcggctgg gtgctgctca aaccgctgaa
     9961 ggtgccggag aaccaggaag gcgggcaggg accgcagagc tgaccccgcc gccccctcgc
    10021 cgggcgcccc ggcgcccgag ccgaccccgc cgccgcccgg cggccgaccg ctccagcgga
    10081 accggccgcc gggcggcttt ttcgcgcccg tcgggcaccc gcaccggccg cccgacgggt
    10141 gctgccggcg cctatgggcg gtgtttgcct cccgaggggc ggggtagcgg ccggtcgccc
    10201 caacgagcac ggcggtcgca tccgcgtgcc cgtcggcccg accagtgcgt tgcaagcaga
    10261 ggagaggcga tgggcgttcg cacgctgatc gagtcatggc cggtctaccg gcagctgacc
    10321 ggagacgacc gatccggccg cggtgcagcg gccaagtccg cgggcagcca caagctgcgg
    10381 gcgcgtacgg ccgaggccga ccgggtcgtg aagtcggtct gcccctactg cgcggtcggc
    10441 tgcgcgcaga acatctacgt ccgcgacgag cagatcgtgc agatcgaggg cgaccccgac
    10501 tcgcccgtca accggggccg gctgtgcccc aagggctcgg ccagtatgca gctcaccacg
    10561 gggtcctcgc gtgagcacca ggtgctgtac cggcccccgc acgcctccga ctggcaggag
    10621 atcgacctgt gggaggccat gcacatggtc gccgaccggg tgctggaggc ccgccgcgag
    10681 ggctggcagg aggaagagaa cggggcacgg acctcgcgaa ccatgggcat cgccagcctc
    10741 ggcggcgccg tgctggacaa cgaggagaac tacctcatca agaagctgct gaccgcgctg
    10801 ggcgtggtgc agatcgacaa ccaggcgcgg gtgtgccaca gctcgaccgt ggcggggctg
    10861 ggcacctcgt tcggccgcgg cggcgccacc acgttcatgc aggaccagca gaacgccgac
    10921 tgcatcgtca tcgagggctc caactacgcc gaggcccacc ccgtcgggtt ccagtgggtc
    10981 atggaggcca aggcccgcgg cgccgtggtc atccacgtcg acccgcactt cagccgcacc
    11041 agcgcactgg ccgacatgca cgtgcccatc cgcgccggca ccgacatcgc cttcctcggc
    11101 gccatcatca accgcgtcat caacgaggag aagtacttca aggagtacct gctcaacttc
    11161 accaacgcgg ccaccatcgt caacgaggag ttccgcgaca ccgaggacct cgacgggctg
    11221 ttctccggct tcgacgagga cgcccgcacc tacgaggtga gcaggtggcg ctacgagggc
    11281 gccgacatcg cgccggcctc cggcaagcgc gaccagctct tcgaggagcg cgtctccaag
    11341 gagcacgagc gctcgcgcca cagctggggc ggagcccgcc ccgagcagca gggctcgggc
    11401 ggcgcaccgg tgggcggcac tcccgaagtc g
//
