LOCUS       NZ_CP045729            25517 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Limosilactobacillus gastricus strain LG045 chromosome, complete
            genome.
ACCESSION   NZ_CP045729
VERSION     NZ_CP045729.1
KEYWORDS    RefSeq.
SOURCE      Limosilactobacillus gastricus
  ORGANISM  Limosilactobacillus gastricus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Limosilactobacillus.
REFERENCE   1  (bases 1 to 1839488)
  AUTHORS   Kim,K.
  TITLE     Genome sequencing of Lactobacillus gastricus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839488)
  AUTHORS   Kim,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) College of Health Science, Korea University,
            Anam-ro 145, Seoul 02841, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Analysis v. v2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.33x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009648555.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 02:36:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,901
            Genes (coding)                    :: 1,756
            CDSs (with protein)               :: 1,756
            Genes (RNA)                       :: 68
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 114 of 145
            Pseudo Genes (incomplete)         :: 26 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 6 of 145
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:41:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1550818
            Orig. end                         :: 1576335
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045729.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..25517
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="cds(LANC_like and DUF4135)"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..25517
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="neighbouring"
                     /product="lanthipeptide-class-ii"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..22943
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22943
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12943](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12943
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    complement(488..497)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.6"
                     /tool="antismash"
     gene            complement(1513..2055)
                     /gene="hpt"
                     /locus_tag="LG045_RS07855"
                     /old_locus_tag="LG045_07855"
     CDS             complement(1513..2055)
                     /EC_number="2.4.2.8"
                     /GO_process="GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254280.1"
                     /locus_tag="LG045_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07855"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="WP_056937933.1"
                     /transl_table=11
                     /translation="MENDIERVLFSEDQIQARIKELATQINQDYANSKELVVVSVLTGG
                     MMFTIDLFKRISLYASLDCVDLSSYRGATTTSSGTVEVLQDLKHPIAGKDVLVVEDIVD
                     TGRTLKKLMDVLAARGANSIRVCTLFDKPERRVVDVKADYVGLDVPNEFLVGYGLDYNN
                     LYRNLPYVGVLKPSIYQ"
     gene            complement(2045..3397)
                     /gene="tilS"
                     /locus_tag="LG045_RS07860"
                     /old_locus_tag="LG045_07860"
     CDS             complement(2045..3397)
                     /EC_number="6.3.4.19"
                     /GO_function="GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="tilS"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /locus_tag="LG045_RS07860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07860"
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="WP_056937928.1"
                     /transl_table=11
                     /translation="MRGLQASIQKALKKYWDGQSKLVVAVSTGVDSMLLLHELEQLHYP
                     IVVAHVNHHLRTQSDQEAQFIAQYCREHHIAIQIADWHVADHPDKGVEEAARQFRYRFF
                     SQVMDEEQSRILLTAHHQNDLAETVLMKLVRGGWLKTIVGLQTARTFNNGWLLRPWLQI
                     SKEELLRQAQVIGLTWYEDQSNQSMAVQRNRFRHQILPALIKEDPKALAHLGEFHDQLT
                     KLLTFAEAELSKYDVVLLDGQRLKVIEFNSLPAATQDLYLKHYFNQAHLQRLTKDQLNQ
                     GLHLLTNQNKPQGQLDLSADWRLVKTYQYAWLQKIKSAETLNSPADVAMIELEKHYFTD
                     DGQPFWFSKHKSTDHLIASFNLTMEQLPLRIRKWQPGDRLRLKNGGHQKIHRILIDQKV
                     PQSKRTHQQVVVDQFDQVLWLVGRKTAWETSRETTQEIYLYYQGDNHDDGK"
     gene            complement(3446..3802)
                     /locus_tag="LG045_RS07865"
                     /old_locus_tag="LG045_07865"
     CDS             complement(3446..3802)
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /locus_tag="LG045_RS07865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07865"
                     /product="septum formation initiator family protein"
                     /protein_id="WP_153672866.1"
                     /transl_table=11
                     /translation="MSQHKSQVIHLNNPYIQRVNHRRHVVRDTHVKRIKMILTLFAAVI
                     LFLGFELWQSHTELAQVNQSIVKTEQTIKSQKSRGQTIQEELELLKNPDYVQQLVRQKY
                     DYSKDGETLYKFVN"
     gene            complement(3910..4182)
                     /locus_tag="LG045_RS07870"
                     /old_locus_tag="LG045_07870"
     CDS             complement(3910..4182)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007121688.1"
                     /locus_tag="LG045_RS07870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07870"
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="WP_056937926.1"
                     /transl_table=11
                     /translation="MRLDKYLKISRIIKRRPIAKKIADQGRIMINDKVAKSSSSVVTGD
                     KVTIKFGNKTLVVKVKQLLDSTKKEDAEQMYEILEEKYEQDFREI"
     gene            complement(4179..7706)
                     /gene="mfd"
                     /locus_tag="LG045_RS07875"
                     /old_locus_tag="LG045_07875"
     CDS             complement(4179..7706)
                     /GO_function="GO:0003690 - double-stranded DNA binding
                     [Evidence IEA]"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="mfd"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007124924.1"
                     /locus_tag="LG045_RS07875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07875"
                     /product="transcription-repair coupling factor"
                     /protein_id="WP_056937925.1"
                     /transl_table=11
                     /translation="MADLTTLLQATNDFNKIVQVDQNHQAQLVTGVDGSFSTVLLTALQ
                     STVGRPQLVVTDSLFHLQELVNDLQNLRPDQEIYAFPVEEVLATEMATSSPDFRLQRLQ
                     ALNALQSQVSPLVIVSAAGLRRRLPDPQFFKTLPLSIHVGDDFEIDDLRQRLSTMGYRP
                     EKMVLRPGDFAIRGSIVDIYALNTTHPVRVDFFDTEVDSLRYFEVESQRSIENITKIQV
                     LPATELVASSEQMDQFKGKLAQELANLPAGEDEESHQAAVDNLQNLFDEVTAGRLPNEA
                     LNYADWLYPDGYGILDYLTPETTIVLDDWARIKEMVQTIDDQERKWIASQVQNQQRVHQ
                     ADLANDQALTIPQSKHSQLYLSRFQKGLGRVKLAQLIDLHVRPMQQFFGQLDLLKTELS
                     RWQAQGQTVIILANSPERQRQIATMFQDFDLPLTETTSDQILTGQIQIVTGTLQHGFEF
                     PKANLVVITESELFKQLQVRQSRRQKLANAERIKSYNDLKPGDYVVHVNHGIGIFTGIQ
                     TMVVDGVHQDYIVLNYRDHAQIFVPVTQLNLVQKYVSADNKEPRINRLGGTEWAKTKRR
                     IAAKIEDIADELVDLYAAREAAPGYAFPQDDYLQEQFDAAFPYTETPDQLRSVDEIKQD
                     MEKPKPMDRLLVGDVGYGKTEVAMRAIFKAVEGGKQVAFLVPTTILAQQHFETLQTRFA
                     EFPVKIAVLSRFLTPKESTATLEQLAAGEIDVIVGTHRLLSKDVQFHDLGLLIVDEEQR
                     FGVKHKERLKQLKASVDVLTLTATPIPRTLHMSMLGVRDLSVLETPPTGRYPIQTYVLE
                     QNPVVIQDGIRRELQRGGQVYYLHNRVHDIEKVVAEISQLVPEARAGYIHGQMSETQLE
                     GVLYDFIRGEYDVLVTTSIIETGVDISNVNTLFIENADRLGLAQLYQIRGRIGRTNRVA
                     YAYLMYQPNKVLTEQGEQRLSAIRDFTELGSGFKIAMRDLSIRGAGNLLGKQQHGFIDS
                     VGYDLYTSMLTEAVAKRRGKKVTPHSNAELDLGIEAYLPDEYVPDQRQKMELYKQVRQA
                     QTAEAMLDLEGDLIDRYGDYDQPVKNLLLVGQLKAVADQAMLTKIERRQDNLYLTFTSE
                     GSQQIKAAQIMAELAKTKFKATMSESDSGQLQVRLIIQPKMTEIDWLNQLLSLVTGIAQ
                     QIQSEEAEE"
     gene            complement(7715..8275)
                     /gene="pth"
                     /locus_tag="LG045_RS07880"
                     /old_locus_tag="LG045_07880"
     CDS             complement(7715..8275)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="pth"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674660.1"
                     /locus_tag="LG045_RS07880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07880"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_056937924.1"
                     /transl_table=11
                     /translation="MKMIVGLGNIGSQYDETRHNCGFMVVDQMARDYHLGAFKHEKAPA
                     VTVTGMINGHKVMLVKPTTFMNDSGRAVKPLMDYYDLDLEDLVIVNDDLDMPVGQVRLK
                     AKGSAGGHNGLRSIIDHLGTQQFNRVKVGIDHPQQGTVVSHVLGKFTTTERATIDDSIE
                     VAENALVDWIDGATFKELMNNYN"
     misc_feature    8339..8352
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.75"
                     /tool="antismash"
     gene            8464..9099
                     /gene="cbpA"
                     /locus_tag="LG045_RS07885"
                     /old_locus_tag="LG045_07885"
     CDS             8464..9099
                     /codon_start=1
                     /gene="cbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674658.1"
                     /locus_tag="LG045_RS07885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07885"
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="WP_007121690.1"
                     /transl_table=11
                     /translation="MILEDLIQPLNKLTTVKEDATLAEALDILEDTGYRCVPILDESEK
                     IFRGNIYKMHLYRHKSRGGDMNLPVTALLKNATKFISIDDDFFTVFFTLRDLPYIAVLD
                     HQKHFYGILTHNHLINMLSAAWNVNTGSYVLTIQANEERGFLVSVAKEITRYCQIANVM
                     TLTPTGSKQLIVTLPEDVTPELFAKITNRLAKKHIQVLHIEDLRQAKL"
     gene            complement(9143..9895)
                     /locus_tag="LG045_RS07890"
                     /old_locus_tag="LG045_07890"
     CDS             complement(9143..9895)
                     /NRPS_PKS="Domain: Abhydrolase_1 (26-134). E-value:
                     9.1e-10. Score: 30.5. Matches aSDomain:
                     nrpspksdomains_LG045_RS07890_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007121691.1"
                     /locus_tag="LG045_RS07890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07890"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_056937923.1"
                     /sec_met_domain="PF00561 (E-value: 1.8e-08, bitscore: 31.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.9e-07, bitscore:
                     28.7, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2.3e-06, bitscore:
                     24.0, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIEIQRDGLTLRGELSGTDTLKNETLVVMMHGFKGSIGYDIANL
                     LFDTHNQLNLHGLPTLRINFNGCGDSDGEFKDMTVLNEIQDGIAILEYVQKVIQPRHLY
                     LVGHSQGEVVASMLAAYYHDQIEKLVLLAPAATLVSDAQAGICQGVSYDPQHVPDSIML
                     DGFEVGGAYFRTAQHLHIYETAQGYQGPVLLVHGLDDQVVSYEATKKYDRIYQHDQMHL
                     IAGASHNLMGDQDQRQVVKDLVTRFLME"
     aSDomain        complement(9494..9817)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LG045_RS07890_Abhydrolase_1.1"
                     /evalue="9.10E-10"
                     /label="LG045_RS07890_Abhydrolase_1.1"
                     /locus_tag="LG045_RS07890"
                     /protein_end="134"
                     /protein_start="26"
                     /score="30.5"
                     /tool="antismash"
                     /translation="LVVMMHGFKGSIGYDIANLLFDTHNQLNLHGLPTLRINFNGCGDS
                     DGEFKDMTVLNEIQDGIAILEYVQKVIQPRHLYLVGHSQGEVVASMLAAYYHDQIEKLV
                     LLAP"
     CDS_motif       complement(9539..9583)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LG045_RS07890_0001"
                     /evalue="1.10E-04"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="LG045_RS07890"
                     /protein_end="119"
                     /protein_start="104"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LVGHSQGEVVASMLA"
     gene            10001..12943
                     /gene="lanM"
                     /locus_tag="LG045_RS07895"
                     /old_locus_tag="LG045_07895"
     CDS             10001..12943
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="LG045_RS07895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07895"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_191992224.1"
                     /sec_met_domain="DUF4135 (E-value: 6.4e-82, bitscore:
                     272.4, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 4.5e-32, bitscore:
                     108.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNQLIYNALTIPEKINHVTYKDNNPQYFNQWNSINSLVNPDVLN
                     EICHSYKIEYHNFPIYFQPYNSVMSNTLILSFDQEFQKMIESFDFTHIRQFDLGTLYLP
                     FIDYVLNQVKASLPNKININESCFDKIVEYLNNELFTLCGKVQALELHKYKLNHSNDKK
                     LDLINYVKNQYQSKLTITKFFQNYPTLMRLVVHRLNKIIQNFTNLFQRIANDQTDIINI
                     LSINAPLVINDLQLNAGDSHSGAQSVIIITLNEQKKLVYKPRNLQVLSQFKKLCEWINI
                     QAKQTLLPINFIPAGIYRSDYSYIKFLSPMLTENHADCLHYYERYGYLILLSYLLGITD
                     LHLENIIAINDTPYLLDLETIFQNKVVDFNDAVSNLFYDLNAESINASCLLPTKLMVNE
                     HESIELSAFKGKGTKLKSSFLGPVDLDNLNFHFTKKKGSYFAGGDNIPKDTNGNEINYL
                     KYRHAIIWGFNKMANFIFSNRNNFINEIKKFSEFQIRILTKSTERYASMLRYANHPNYN
                     HQMQYREKLLFNTWVYPYHDKSIIKSEVKDLIYNDIPFFYTQVKSTSIFGSDGTEYPKY
                     FEKSGLQLAINRIHNFSEVEYENQKTILYDALDMTDELICHYTNQIKETENDQFDYISI
                     AERIAHQIMDLAYKDDQNCILKTFDYSPDHGWRIYPTNENLYCGLSGISIFFIHLYQHT
                     EKRVYQDFFNNSLHQAIEFANLQPIKSAYSGFLAPLYPLLLIATKSRSYSSQEIHYLNQ
                     LESKLNSYIETINDQTKLDFMGGLGGVVYLLNLQKQVWNDLYVSNMTLKRLNNIFSKNV
                     IKFTNVFGFAHGFSGLSLPLYLMGRQDLAIKLLKKEAKLDEPQIFDQAKWCRGIPGILM
                     VKALLNQQIDLMVPAEAFEKIDDTLCHGKCGLISSYLYLSRKLNNQYFYNYAFIMANAM
                     LSDYLSGNEFKLKLTSNSFQPGLYDGLSGIGLTILSLSYDDIKNPLIFEL"
     gene            12966..13160
                     /locus_tag="LG045_RS07900"
                     /old_locus_tag="LG045_07900"
     CDS             12966..13160
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LG045_RS07900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LG045_07900"
                     /product="hypothetical protein"
                     /protein_id="WP_056937921.1"
                     /transl_table=11
                     /translation="MDANKKIDTVSGSPFESLSIEEMTKIQGSGSQNMMTVSPVATPIA
                     SFQASEYISHKINKFLHRN"
     gene            13173..13373
                     /locus_tag="LG045_RS07905"
                     /old_locus_tag="LG045_07905"
     CDS             13173..13373
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369960.1"
                     /locus_tag="LG045_RS07905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07905"
                     /product="hypothetical protein"
                     /protein_id="WP_056937920.1"
                     /transl_table=11
                     /translation="MDSKQQFNDTLGEAFEELNIDEMTKIQGTGFESLSISWPIVTYFE
                     ISLSLAHQVNVYSHLRKKRNK"
     cand_cluster    13364..25517
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    13364..25517
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[18363:20517](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(18364..20517)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     gene            13395..13613
                     /locus_tag="LG045_RS07910"
                     /old_locus_tag="LG045_07910"
     CDS             13395..13613
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0005488 - binding [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03893.1"
                     /locus_tag="LG045_RS07910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07910"
                     /product="lichenicidin A2 family type 2 lantibiotic"
                     /protein_id="WP_056937919.1"
                     /transl_table=11
                     /translation="MSKEELNSIVGRAFEEMDIQQMTSIQGSGTETAQSTPAISRVTLS
                     IARKSSAKCISWISFSAGGLNSYKSKC"
     CDS_motif       13395..13478
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MSKEELNSIVGRAFEEMDIQQMTSIQGS"
                     /locus_tag="LG045_RS07910_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       13479..13613
                     /prepeptide="core"
                     /locus_tag="LG045_RS07910_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.70"
                     /molecular_weight="4341.9"
                     /monoisotopic_mass="4339.4"
                     /core_sequence="GTETAQSTPAISRVTLSIARKSSAKCISWISFSAGGLNSYKSK
                     C"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSKEELNSIVGRAFEEMDIQQMTSIQGS"
                     /leader_location="[13394:13478](+)"
                     /alternative_weights="4359.9"
                     /alternative_weights="4377.9"
                     /alternative_weights="4396.0"
                     /alternative_weights="4414.0"
                     /alternative_weights="4432.0"
                     /alternative_weights="4450.0"
                     /alternative_weights="4468.0"
                     /alternative_weights="4486.1"
                     /alternative_weights="4504.1"
                     /alternative_weights="4522.1"
                     /alternative_weights="4540.1"
                     /alternative_weights="4558.1"
     gene            complement(14105..>14467)
                     /locus_tag="LG045_RS07915"
                     /old_locus_tag="LG045_07915"
                     /pseudo=""
     CDS             complement(14105..>14467)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680947.1"
                     /locus_tag="LG045_RS07915"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07915"
                     /product="integrase core domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PLVEKAKQNRHIDQPLIMHSDRGSQFTSEAYNQATAEMTLSYSKK
                     SYPGDNACIESFHALIKRKWLNRFKIHSYSEAKRLVFQYIETFYNTVRTHSRCEFKSPK
                     QFEDEYQTLIQNLTAA"
     gene            complement(14550..15332)
                     /locus_tag="LG045_RS07920"
                     /old_locus_tag="LG045_07920"
     CDS             complement(14550..15332)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="LG045_RS07920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07920"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_056937917.1"
                     /transl_table=11
                     /translation="MTINVLLVENNLEQLEIMRTVILNRIAINPTLKAYDMHIALCTSD
                     PYETIDYIRCNKFEDYFGVLDIDLNSDVSGIEIAEELRKTNGFTEICYVSAYEDFLLKT
                     INSNTKPIDFIYKSQGIEQMIHQLRRAIDLVYFNYTKLVTSHNMGNMFTYEPFIGLIKR
                     VPVDEILYIKTTNKQHKLELVCKDRTVEFYGELKSIEAKRNNFIRVDRQILINLDNVIS
                     LNVKERRVYFLTSSDEMAFCKVSLRKIPAIKRILLSEK"
     gene            complement(15322..16641)
                     /locus_tag="LG045_RS07925"
                     /old_locus_tag="LG045_07925"
     CDS             complement(15322..16641)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="LG045_RS07925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07925"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_056937916.1"
                     /transl_table=11
                     /translation="MIMVGVVQYFLMEIMSLASCYFFSVLNSSIKEKKIFFGGVVLTVL
                     CLGVFGNFIYLVYTLFVVLYNRLMKKESLLCFNNGIVYIGAIFSLTVGGCISFLFNFVL
                     IPNASVNKLITCQLVSESVVLVILYFYRRYVQIGLSKLRIYLYRHKLTKFFQCSILLAY
                     IFILSLQIIAEELGVEMRIEGGLMLISMIIILIVLGFTISLSKYYNRVNQLEYVKRGVS
                     QSQEYLAFNRRDYLRLRKLRHKYVNQLISLDGLLENGDYLAAKGYLNNILKEFNQSINS
                     EINRQYQILNELSSVELRSLIFSKVQLMHEYNISCNLSVNTDFPISEENAIDVVTVIGS
                     LLDNAIEETRDKYQSRIELELNQYGSEILDFKVVNTIDHEIDNHKLFQNEYSTKTGHSG
                     IGLTIIKDIIDSSDNYALEIETYKDRIQFDYMIRRDPDDN"
     gene            complement(16907..17623)
                     /locus_tag="LG045_RS07935"
                     /old_locus_tag="LG045_07935"
     CDS             complement(16907..17623)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="LG045_RS07935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07935"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_153672867.1"
                     /transl_table=11
                     /translation="MRRITVLNGSVNRKGNTQYFIEEILRGIPKEIDRQFFFASDYMFP
                     LVYKGLYDPEYSRKVAGDNIYSLQESILESNLLIIASPVYMHNISSNLKLVIEKLSTWS
                     HILRLDGKPVVVLSTCSTNGDTTVTSYLCKVISTMGGNIIANCNANGFQLHDEVWLNDV
                     TNKIRSKIICSIGNSLQSNYYIEEVFKGMKLIMNDRIGSDVPNSFAEEESTYWVKSGLI
                     NYDSFQKFLDDKYNLK"
     misc_feature    complement(17600..17615)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.41"
                     /tool="antismash"
     misc_feature    17617..17631
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.62"
                     /tool="antismash"
     CDS             complement(17844..18053)
                     /gene="allorf_1568662_1568871"
                     /locus_tag="allorf_1568662_1568871"
                     /protein_id="allorf_1568662_1568871"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MVNMLDSELKKIVGDSFEDMSVQQMTSIQGNGAESAQTSPLIPIS
                     KASSVRCAKWVSAISGLSWAKHHK"
     CDS_motif       complement(17979..18053)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MVNMLDSELKKIVGDSFEDMSVQQM"
                     /locus_tag="allorf_1568662_1568871_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(17844..17978)
                     /prepeptide="core"
                     /locus_tag="allorf_1568662_1568871_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.40"
                     /molecular_weight="4392.9"
                     /monoisotopic_mass="4390.4"
                     /core_sequence="TSIQGNGAESAQTSPLIPISKASSVRCAKWVSAISGLSWAKHH
                     K"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MVNMLDSELKKIVGDSFEDMSVQQM"
                     /leader_location="[17978:18053](-)"
                     /alternative_weights="4411.0"
                     /alternative_weights="4429.0"
                     /alternative_weights="4447.0"
                     /alternative_weights="4465.0"
                     /alternative_weights="4483.0"
                     /alternative_weights="4501.1"
                     /alternative_weights="4519.1"
                     /alternative_weights="4537.1"
                     /alternative_weights="4555.1"
                     /alternative_weights="4573.1"
     gene            complement(18064..18243)
                     /locus_tag="LG045_RS07945"
                     /old_locus_tag="LG045_07945"
     CDS             complement(18064..18243)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0005488 - binding [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03893.1"
                     /locus_tag="LG045_RS07945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07945"
                     /product="lichenicidin A2 family type 2 lantibiotic"
                     /protein_id="WP_056937913.1"
                     /transl_table=11
                     /translation="MEKDINTVVGDPFEELTIDEMTKIQGNGTQSAQSTPVVESLAESF
                     ALSYAISRAFKHSK"
     gene            complement(18364..20517)
                     /locus_tag="LG045_RS07950"
                     /old_locus_tag="LG045_07950"
     CDS             complement(18364..20517)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="LG045_RS07950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07950"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_056937912.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.5e-36, bitscore:
                     121.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 9e-241, bitscore:
                     797.9, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYKYYTVQMDEEDCGVAALSMVLKNFGTNVSIAKLRTSTKTTID
                     GTTIYGIVQAAKIYGLDTLAIKANRQLFEINHLPLPFIIHVIRKESYLHYCVVTEINGD
                     QITVADPDDSVGIKRLSIKKVCEEWSGNAILFSKGDDYQSINEKSAGIFEYIRYLTKNR
                     FVTCGIVLMAALVLLTNIINSLAIQLVIDRLIPGQAFNKLTFLVIGLISMYFFNATFSF
                     VESIAMTILGQRLSKDIALNFIKHLFDLPIDFFATRRTGDVVSRFNDTNRVIEALSRSI
                     VTIFLDVLIVMITGYVLGLLNFRLLLIIGLFIPIYTVTIVTFASIFEKMNKKVMIKNAK
                     LSSLIIDSIKGMETLKCLNANVKQLDKIKLAFNDFLNELKKYSILTSSQESIKQFLQLT
                     LNILVIWIGANQIMNNYFKLGQLIAFTSLLGYFTNSLKNILDLQSNLQSAIVATRRLND
                     ILIVPVEKEIQLKTVSNDISGDIVLSGVQCRYGYQDLVLKGIDLEIKKGNKIAIVGTSG
                     SGKSTIAKLLVGFLTASQGSITLNGINIEKISRSLLRSEINYVPQYSYIISDTIWNNLT
                     LGCSDKIDREKVEKACRIAMISETINALPLGYETKLDEEATILSGGQKQRLTIARAILS
                     NAQTLILDESTSSLDTITERELINNLLNLKDKTIIFIVHRLKVAKQVDDIVVIENGTVV
                     ERGSHNQLMAQHKRYYDFVRLRS"
     gene            complement(20669..21790)
                     /gene="alr"
                     /locus_tag="LG045_RS07955"
                     /old_locus_tag="LG045_07955"
     CDS             complement(20669..21790)
                     /EC_number="5.1.1.1"
                     /GO_function="GO:0008784 - alanine racemase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="alr"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007121692.1"
                     /locus_tag="LG045_RS07955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07955"
                     /product="alanine racemase"
                     /protein_id="WP_056937911.1"
                     /transl_table=11
                     /translation="MVTANLRDAQVEVNLAAIRHNVAAQRQAMPAGNRIFGVVKANAYG
                     HGAVAVAQDLSSAGIDGFCVAALDEGLELRQAGLTELILVLGITPVQYAGTAAENDISL
                     TVGDVDWLKAYQQLAHAVSIPKPLKIHLGFDTGMGRIGFTEPALLKEALELLKDPVFEF
                     EGCFTHFATADEANDAYFKQQIARWHELLAVLPTLPPYVHMANSATGLWHPDQITGNTV
                     RMGISMYGLNPSGTVLPATLDLQPALAVTARLTFVKQLKAGRSVSYGATYTAKQDEWIG
                     TIPVGYADGYTRQLSGYQVLINGQRCDLVGRVCMDQMMVRLPQELPVGTKVTLLGRDGN
                     DEITATELAEKTGIINYEILTSLSPRLKRIYKS"
     gene            complement(21777..22148)
                     /gene="acpS"
                     /locus_tag="LG045_RS07960"
                     /old_locus_tag="LG045_07960"
     CDS             complement(21777..22148)
                     /EC_number="2.7.8.7"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (4-72). E-value: 3.3e-17. Score:
                     54.6. Matches aSDomain:
                     nrpspksdomains_LG045_RS07960_ACPS.1"
                     /codon_start=1
                     /gene="acpS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007121693.1"
                     /locus_tag="LG045_RS07960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07960"
                     /product="holo-ACP synthase"
                     /protein_id="WP_056937910.1"
                     /transl_table=11
                     /translation="MIKGIGIDITEIKRVQAAVNKHAQFIPKVLTPAEQAQLENLSGQR
                     YWEYISSRFSLKESFSKAMGTGIGTQVGFQDISFINNDLGRPEVFQSIFSGRAHASVSH
                     TDQLVMTEVILEEGSDGNS"
     aSDomain        complement(21933..22136)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LG045_RS07960_ACPS.1"
                     /evalue="3.30E-17"
                     /label="LG045_RS07960_ACPS.1"
                     /locus_tag="LG045_RS07960"
                     /protein_end="72"
                     /protein_start="4"
                     /score="54.6"
                     /tool="antismash"
                     /translation="IGIDITEIKRVQAAVNKHAQFIPKVLTPAEQAQLENLSGQRYWEY
                     ISSRFSLKESFSKAMGTGIGTQV"
     gene            complement(22151..23647)
                     /locus_tag="LG045_RS07965"
                     /old_locus_tag="LG045_07965"
     CDS             complement(22151..23647)
                     /EC_number="3.6.4.-"
                     /GO_function="GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003667239.1"
                     /locus_tag="LG045_RS07965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07965"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_007121694.1"
                     /transl_table=11
                     /translation="MYFSELGLSNKLLKAVQRSGYEEATPIQEQTIPMVLKGLDVIGQA
                     QTGTGKTAAFGLPVLEHVDTQSSAIQALIISPTRELAIQTQEELYKLGKEKRARVQVVY
                     GGADIRRQIKALKNGPQIIVGTPGRLRDHLNRGTIKLDQLQTLVLDEADEMLNMGFLDD
                     IEAIIQATPSDRQTLLFSATMPPEIKRIGVQFMKDPQTVKIKAKELTTDLVDQYYIKAR
                     DYEKFDIMTRLIDVQDPDLTIVFGRTKRRVDELSRGLIARGYNAAGIHGDLTQDRRSKI
                     MKRFKNGELDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAGNHGVSL
                     TFVTPNEMDYLHEIEKLTKVRMLPLKPPTAEEAFRGQVASAFNDINELIERPDNDRYRE
                     AASELLATHEAVDLVATLLNELTKDDETQVPVKITPERPLPNRKRKPNRNGGNHYRRRS
                     NGGNRRGDNRNRRPRGGNGSHRNPRSQGKRNSFKIRRKED"
     gene            complement(23660..25041)
                     /gene="murF"
                     /locus_tag="LG045_RS07970"
                     /old_locus_tag="LG045_07970"
                     /pseudo=""
     CDS             complement(23660..25041)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686468.1"
                     /locus_tag="LG045_RS07970"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LG045_07970"
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKMKLAEIAKAIDAQDINAAWDELEVTSVAFDSRNLTPGSLFVPL
                     QGARDGHEFVAQAFQNGAKATLWAADHDIPSDDQFPRLVVKDPLAAFQRLSQYYLKKIN
                     PIVVAVTGSNGKTTTKDMVAAILATQFNVTKTFANFNNEIGVPVTLLNMEPSTEVVVVE
                     MGMDRFDQLDLLSRLATPDYAVITMIGEAHIEFFGTRDRIADAKMEIVHGMNEDGTLII
                     NGDEPLLTARAEDIEQQVVTFGRQLADDFYATSVQLADDQVAFSVNEWPDLTFTLPMVG
                     EYNVNNALAAIAVGAQLQITPENMQKALQGLQMTENRAEWIQGTNGERILSDVYNSNPT
                     AVAEVLKTIEQVQTEGKKVIVLGDMLELGTASAQLHAQLAAEIDSDQIAEVYLVGDEMQ
                     ALLKQLTADGFPNKRLHHYDTNALAQLTKDLNATLRTDDIVLLKGAMAFIWKRSCKDSR
                     KD"
ORIGIN
        1 gtcactttat gaacaacccg agcgtcatta aggactaagc ccacaatcgt atgcccagct
       61 tcgtggtaag caactgtttc ccgttcttgc ttggaaatta cccggtctcg cttagccggt
      121 ccagcaataa cccgatcttc ggcttcgtcc atatccaacc gatcgatttg atccttattc
      181 cgacgcgctg ctaacaaggc cgcttcgttt aagaggtttt ccaaatcagc cccgacaaag
      241 ccaggagttt gcttggcaat ttctttaaga tcgacatctg ccgccaatgg cttgttttta
      301 gcatgaaccc gaaggattgc ttcccgaccc ttaacgtctg gtcgaccaac cagcaccttc
      361 cggtcaaatc ggcccggccg caaaagggcg ggatcaagta catcagaacg gttagtagcc
      421 gccattacaa taacgccttc gtcgccttgg aaaccatcca tttcaactaa caattggtta
      481 agcgtttgtt cccgttcatc atgaccgcca ccttgaccat tgccccggcg ccgaccaaca
      541 gcatcaattt catcaataaa gatgattgat ggcgctgatt ttttagcctg atcaaacaga
      601 tcccggaccc ggctagctcc gaccccgaca aacatttcca caaagtcaga accagaaatt
      661 gaaaagaatg ggacgccagc ttcaccggca acggccttcg ctaggagagt cttcccggta
      721 ccaggaggcc cctctaacag aaccccagcg ggcatccgtg cccccagccg tgagaatttc
      781 tttggattct ttaagaattc aacaatttca actaattctt gcttctcttc ttcttctcca
      841 gcaacatcac caaagcgaac cttgttcttc tttggatcag atggtttggc tttggacttg
      901 ccgacattca taatgcctct gccaccgccg ctaccaccac cagcttgtcc catcatcatg
      961 tagaagaaaa taataatgat gactagcggt aatagggata ctaacagatt tgaccagata
     1021 ctattggact cttcaccttt agtactaatc ttagtgttag tttggttcgc gtaacgttga
     1081 atttccgaaa ccgtcgcgtc attttcaagc accgacgttt caaattgttt tgtcgtcgta
     1141 cttgaagatg cacctaagga caaaccagtc gttgaactag aactggttgt ctttgccttt
     1201 ttgtattccc ccgttactcg gtatacgcca ttgcttggct gcaattggac actcttaact
     1261 tcacccttgc gcagttcctt aatgaacgta ctttgcgaaa cctctttttg ttcaccagtt
     1321 aaacttgacc ctccaaagaa gaagtacaca ccggccataa tacagaaaaa gattatcgcg
     1381 taaaaaagga tgttatgagt gaacccattg ttcctccgat tgttgttcat aacattctcc
     1441 tattccaaca ttatttgatg gtcactatca tagcacaacc acccgcggtt gactattatt
     1501 ttaatcacga gatcactgat aaattgatgg tttcaaaaca ccgacatatg gcagatttcg
     1561 atataaatta ttatagtcaa gtccataacc aactaagaat tcattaggaa catccagacc
     1621 tacataatcg gccttaacat caaccactcg acgttccggt ttatcaaaca aagtacatac
     1681 tcgaatacta ttggcaccac gcgccgccaa aacatccatc aattttttta atgtccgtcc
     1741 agtatcaacg atatcttcaa caacgagcac atctttaccc gcaattggat gcttcagatc
     1801 ctgcaatact tctaccgtcc cactactagt agttgttgcc ccacgatagc ttgacaaatc
     1861 tacacaatct aacgaggcat aaagactgat ccgtttaaag aggtcgatcg taaacatcat
     1921 cccccctgtt aaaactgaaa ctaccactaa ttccttagaa ttagcgtaat cttgattaat
     1981 ttgagtggcc aattctttaa tccgcgcttg gatttggtct tcactaaata atacccgttc
     2041 aatgtcattt tccatcgtca tgattgtctc cctgatagta caaataaatt tcttgtgttg
     2101 tctctcgtga tgtttcccaa gcagttttac gtccaactaa ccacagaact tgatcaaatt
     2161 gatccacaac cacttgttgg tgagtgcgtt tactttgtgg tactttttgg tcaattagga
     2221 ttcgatgaat tttttgatgc cccccatttt taagccttaa gcgatctcct ggttgccatt
     2281 ttctaattcg taatggtagc tgctccattg ttaaattaaa gctcgcaatc agatggtcag
     2341 tcgatttatg cttactgaac caaaatggct gcccatcatc ggtaaaatag tgcttttcca
     2401 attctatcat cgcaacatca gctggtgagt ttaaagtttc ggctgatttg atcttttgca
     2461 accacgcata ctgataagtc ttaaccagtc gccaatctgc acttaaatca agctggcctt
     2521 ggggcttgtt ttgattagtt agtaaatgga gcccctgatt aagttgatcc ttggtgagcc
     2581 gttgcaaatg tgcctgatta aaataatgct tcaaatacaa gtcttgcgtt gctgccggta
     2641 aagaattaaa ttcaatgact tttaatcgct gaccatctaa taaaaccaca tcatatttgg
     2701 acaactccgc ttcggcaaag gtcaataatt tggtcaattg gtcatgaaat tcacctaaat
     2761 gagcaagtgc ttttggatct tctttaatta aggccggcaa aatttggtgt cgaaaacgat
     2821 tgcgctgaac cgccatggac tgattggatt gatcctcata ccaagttaac ccgattactt
     2881 gagcttgacg gagaagttct tcttttgaaa tttgcagcca aggtcgcagt aaccagccgt
     2941 tgttaaaagt ccgagccgtt tgtagaccga caatagtctt taaccaacca cctcgcacta
     3001 acttcattaa aaccgtttct gccaaatcgt tctgatggtg agcagttaat aaaatccgac
     3061 tttgctcttc atccataacc tgagagaaaa accgataccg gaattggcgc gcagcttctt
     3121 caactccttt gtcgggatga tcagctacat gccaatcagc gatttggatt gctatatgat
     3181 gctcacggca gtattgggct ataaattggg cttcctgatc actttgcgta cgtaaatggt
     3241 gattgacgtg ggctaccaca atcggatagt gaagttgttc caactcatga agtagcagca
     3301 tagagtccac tcccgttgag actgccacca ccagtttgga ttggccatcc caatactttt
     3361 ttaatgcctt ttgaattgat gcttgtagtc cccgcatccg tgacccctct tatgaaaaaa
     3421 gactgggtta ccccagtccc actttttaat taacaaattt atataaagtt tcaccatctt
     3481 tagaataatc atacttttgg cgaactagct gttggacgta gtcgggattc ttaagtagtt
     3541 caagttcttc ttgaatcgtt tgaccacgac tcttttgact cttgatagtc tgttcagtct
     3601 taacaatgga ctgatttact tgggccaatt cagtatgtga ttgccacaac tcgaagccta
     3661 aaaataaaat cacggccgca aacagcgtta agatcatttt gatccgtttt acatgtgtat
     3721 ctcgaaccac gtgccggcga tgattcaccc gttgaatata tggattattg aggtgaatta
     3781 cctgactttt atgttgactc ataaccgaca cccgagacct tcccaaagat ttttaaacca
     3841 tttttagtat aacaatcggt ttcgaaatct gtctaggcat tttccacaaa ttatcagata
     3901 aagttccatt taaatttcac ggaaatcttg ctcgtatttt tcctctaaaa tctcatacat
     3961 ttgttccgca tcttcctttt tcgtactatc taataattgc tttactttca ccactaaagt
     4021 cttatttcca aacttaattg tcaccttgtc ccccgttact accgaactgg atgacttagc
     4081 caccttatcg ttaatcataa tgcgtccttg atccgcaatt ttcttcgcga ttggccggcg
     4141 tttaataatt cgagaaattt ttaagtactt gtctaacctc attcttctgc ctcctcactt
     4201 tgtatttgtt gagctatccc cgtaaccaaa cttaagagtt ggtttaacca gtctatttcg
     4261 gtcatcttag gttggataat caagcgaacc tgaagttggc ctgagtcgct ctcgctcatc
     4321 gttgctttaa atttggtctt agctaattcc gccataattt gagccgcttt aatctgctga
     4381 ctaccctcac tagtaaaagt taaatataaa ttatcttgtc ggcgttcaat cttcgtcaac
     4441 attgcttgat cagctactgc ttttaattgt cctaccagca aaagattctt aactggctga
     4501 tcgtaatcac catagcggtc aataagatcg ccttctaaat ctaacatagc ttctgcagtt
     4561 tgtgcttgcc gcacttgctt ataaagttcc attttctgtc gttgatctgg cacatattca
     4621 tccggtagat aggcttcaat cccaagatct aattcagcgt tagaatgtgg ggttactttc
     4681 ttgcctcgac gtttcgccac cgcttcggtt aacatacttg tatataaatc ataaccaact
     4741 gaatcaataa acccgtgctg ttgctttcca agtaaattac cggcaccacg aattgacaga
     4801 tcccgcatgg caatcttgaa accagatcct aattccgtaa aatcgcgaat ggcggacaaa
     4861 cgttgttccc cttgttccgt taaaacctta ttgggttgat acataagata agcataggca
     4921 acacgattgg ttcgcccgat ccgtccccgg atttggtata actgagctaa gcccagccga
     4981 tccgcatttt caataaagag ggtattaacg ttagagatat caacacccgt ttcaataatc
     5041 gatgtggtca ctaagacatc atattcacca cgaatgaaat catataaaac cccttcaagc
     5101 tgcgtttcac tcatttgacc atgaatataa ccagcccgcg cctccggtac aagctgactt
     5161 atttcagcca cgaccttttc aatatcatgg acccgattat gtaaataata gacttgcccg
     5221 cctcgctgta attcccgccg gatcccgtct tgaatcacga ctggattttg ttccaggaca
     5281 taggtttgaa tcgggtaccg cccagtcggt ggcgtctcca atactgatag atctcttact
     5341 cccagcattg acatgtggag ggtacgtgga attggtgttg ccgtcagtgt caaaacatcc
     5401 acgctagctt ttaattgctt taaccgttcc ttatgtttta ccccaaatcg ttgttcctca
     5461 tcgacgatta ataaacctaa atcatgaaat tgaacatctt ttgataaaag ccggtgggtc
     5521 ccaacaatca catcaatctc accggcagcc aattgttcca aagtggcagt actttccttg
     5581 ggcgttagaa agcgtgacaa tacggcaatt tttactggaa attcggcaaa gcgggtttgt
     5641 aaggtttcaa aatgttgctg tgccaaaatg gtcgtaggga ccaaaaaagc aacctgtttc
     5701 cctccttcaa ccgccttgaa aatggcgcgc atggcgactt cggtttttcc atagccgacg
     5761 tcccccacta acaggcgatc catcggtttc ggcttttcca tatcttgttt gatttcatcc
     5821 acactccgca attgatctgg agtttccgta tagggaaagg ccgcatcaaa ttgctcttga
     5881 agataatcat cctgggggaa agcatacccg ggggcggctt cacgagcagc atataagtcg
     5941 actaactcat cagcaatgtc ttcaatttta gcggcaattc gccgtttcgt cttagcccat
     6001 tcggtgcctc caagacggtt gatccggggt tctttattat cagccgaaac atatttttgg
     6061 acaagattaa gttgggttac aggtacaaaa atctgtgcat gatcccgata attcaaaacg
     6121 atgtagtctt gatgtacccc atcaaccacc atggtttgaa ttcctgtaaa aattccaatc
     6181 ccatggttaa catgaacaac ataatcgcca ggttttaaat cgttatagct cttaattcgt
     6241 tcggcgttag ccaatttttg tcggcgtgat tgacgaactt gcaattgttt aaagagttcg
     6301 ctttcggtaa tcaccaccaa attagctttg ggaaactcaa acccatgctg gagagtgccc
     6361 gttacaattt gtatttgacc agttaagatt tgatcactcg ttgtttcggt taagggtaaa
     6421 tcaaaatctt gaaacatggt cgcaatctga cgttgccgtt caggggaatt agccaaaata
     6481 atcaccgttt gtccctgtgc ttgccagcga cttaactcgg tttttaataa atcaagctga
     6541 ccaaaaaact gttgcattgg ccgaacgtgt aaatcaatta actgggctaa cttgacccgc
     6601 ccgagacctt tttgaaaacg agaaagataa agttgactgt gcttactttg cggaattgtt
     6661 agggcttgat cattggctaa atctgcttga tggacccgtt gttgattttg aacctgactc
     6721 gcaatccatt tccgttcttg atcatcgatg gtttgcacca tctctttgat ccgtgcccaa
     6781 tcatctaaca caatcgtcgt ttctggagtc aaatagtcca agataccata accatcagga
     6841 tacaaccaat ccgcgtaatt taaggcttca ttcggtaatc ggccagccgt cacttcatca
     6901 aataaatttt gtaggttgtc cacggcagct tgatgacttt cttcatcttc acctgcaggt
     6961 aggttcgcca gttcttgggc taacttgcct ttaaattggt ccatttgctc acttgatgcc
     7021 actaattcag ttgctggcag cacctggatc ttggtaatgt tttcaatact gcgttgtgat
     7081 tcaacctcaa aataacgtag ggaatccact tccgtatcaa aaaaatcaac tcgaactgga
     7141 tgagtcgtat tcaaggcata aatatcaaca atcgagccgc gaatggcgaa atcacctggc
     7201 cgtaacacca ttttttccgg tcggtagccc atggtactta aacgttgccg taaatcatca
     7261 atttcaaagt catcgccaac atgaatcgat aagggaaggg tcttgaaaaa ttgcggatcc
     7321 ggcaaacgcc gacgtaaccc cgcagccgac actatcacca gtgggcttac ttgactttgc
     7381 aaagcattta aggcttgaag tcgctgcaaa cgaaagtccg gtgaactggt ggccatctcc
     7441 gttgctaaaa cttcttcaac cgggaaagca tagatttctt gatcaggacg caaattctgc
     7501 agatcattaa ccaattcttg caaatgaaac aaactatccg tcaccactaa ttgcggccgt
     7561 ccgacagttg attgtaaagc cgttaacaac accgtggaaa aggagccatc tactccagtt
     7621 actagttgag cctggtgatt ttgatcaact tggacaattt tattaaaatc gttagtcgct
     7681 tgcagtagcg tcgtcaaatc agccatagga cctcctaatt atagttattc attaattcct
     7741 taaaagtagc cccatcaatc cagtcgacca atgcattttc agctacctca atactgtcgt
     7801 caatggtggc gcgttcggtt gtcgtaaact tacctaagac atgactaacc acggtgcctt
     7861 gttggggatg atcaatcccc actttgacgc gattaaattg ttgggttccc aagtgatcaa
     7921 tgatactccg taagccatta tgcccaccag cagatccctt tgcttttagg cggacttggc
     7981 caactggcat atctaaatca tcattaacaa taactaaatc ttccaaatca agatcataat
     8041 aatccatcaa tggttttacc gcccgtccgg aatcattcat gaaggtcgtt ggttttacca
     8101 acattacctt atgaccatta atcatcccag tgacggtcac cgctggtgct ttttcatgtt
     8161 taaaggcacc caaatgataa tctcgtgcca tttgatccac caccataaat ccgcagttat
     8221 gacgagtttc atcatattga ctaccaatgt tccctaatcc aacaatcatt ttcatcagct
     8281 caatctcctc tcgataaact acaaaaaggc tgcgtgatcg ttgaccaccc agcgttgata
     8341 aaactattat aacttaaatc ttactctaac ccaaaaagtc tgccccgctt ctaatctatt
     8401 tctcatatta agtttccatt atgttatgat attagtatcg atttatttat acggagctta
     8461 attatgattt tggaagattt aattcagccg ctgaacaaat taacgaccgt caaagaagac
     8521 gccaccttag cggaagccct ggacatctta gaagacaccg gttaccgctg tgtgcctatc
     8581 ttagatgaat ccgaaaagat ttttcggggg aatatttata aaatgcatct ctatcggcac
     8641 aaatcccgtg gcggtgatat gaatttacca gtgaccgctt tattaaaaaa cgccaccaaa
     8701 tttatttcaa ttgatgatga cttttttacc gttttcttca ccttacggga tctcccctac
     8761 attgccgtct tagatcacca aaaacatttt tacggtattt taacccacaa ccacttaatc
     8821 aacatgttaa gtgcagcttg gaatgtcaat actggtagtt atgttttaac gatccaagct
     8881 aatgaagaac gtgggttctt ggtgagtgtg gcgaaggaaa ttactcgtta ttgtcaaatt
     8941 gctaatgtta tgaccttaac accgaccgga tcaaagcaat tgattgtcac ccttcccgaa
     9001 gacgtgaccc ccgaattatt cgcaaaaatt accaatcgac tggccaaaaa acacattcag
     9061 gttttacata ttgaggattt gaggcaagcc aagttatagc atcttaaaag acgcccaacc
     9121 atttttggtt gaggcgcctt ttctattcca ttaaaaatcg agtcactaaa tctttgacga
     9181 cttggcgttg gtcctgatcg cccattaaat tatgactagc ccctgcgatt aggtgcattt
     9241 ggtcatgctg ataaattcga tcatactttt tagttgcttc atatgacacc acttgatcat
     9301 ctaatccgtg gaccaaaaga accgggcctt gatatccctg ggcagtctca taaatgtgga
     9361 ggtgctgcgc cgtccggaaa taggcacccc caacttcaaa gccatccagc atgatggagt
     9421 ccggaacgtg ttggggatca tagctaacac cttggcatat tccggcttgg gcatctgaca
     9481 ctaaagttgc ggcgggagca agcaaaacta atttttcaat ttgatcatga taatatgcgg
     9541 cgagcatgct agccaccact tcaccttgag aatgtcccac tagatataaa tggcgaggtt
     9601 ggataacttt ttgcacatat tctaagatag caatcccgtc ttgaatctca ttcaacacgg
     9661 tcatatcttt aaattcacca tcactatccc cacaaccatt aaaattgatc cgtaaagtcg
     9721 gaagaccatg taagttgagt tgattatggg tatcaaaaag aagattagct atatcataac
     9781 caatactgcc cttaaaacca tgcatcatca ctactaaagt ctcatttttt agcgtatcgg
     9841 tgcctgataa ctccccccgt aaagtcagcc catcacgttg aatttctatt tccataagtg
     9901 tcacctcgtt taactcacaa tcaaaagcgt ttaataaaaa ctacattacc atccgtttat
     9961 ttccataata tcaacctaaa cgatacttga gaggatgaca atgatgaatc aactaattta
    10021 taatgctcta acaattccag aaaagattaa ccatgttact tataaagata acaaccctca
    10081 atattttaac caatggaact ctattaatag tttagttaac ccagatgttc taaatgagat
    10141 ttgccattcc tacaaaattg aatatcacaa cttccctatc tattttcaac cctataattc
    10201 tgtcatgagt aataccctta tactctcctt cgatcaagaa ttccaaaaaa tgattgaatc
    10261 ctttgacttt actcatatta gacaatttga cttaggaacc ctatatcttc ccttcattga
    10321 ttatgtatta aatcaggtta aagcatcatt accaaataaa atcaacatta acgagtcatg
    10381 cttcgataaa atagtcgaat atctaaataa tgaacttttt actttatgtg gtaaggttca
    10441 agcacttgag ctacataaat acaaactgaa tcacagtaat gacaaaaaac ttgatcttat
    10501 taattacgtt aaaaatcagt atcaatcaaa gctaacaata actaagttct tccagaatta
    10561 tccaacttta atgcggttag tcgttcatcg tctaaacaaa attatccaga atttcacaaa
    10621 tctttttcaa cgaattgcta atgaccaaac cgatattata aatatattat caattaatgc
    10681 cccgctagtt attaatgatc ttcagttaaa tgcgggtgat tcacactcag gggcacaatc
    10741 cgtcattatt ataaccttaa atgaacagaa aaaattggtt tataaacccc gtaatttaca
    10801 agtcctttca caattcaaaa aattatgtga atggattaat attcaagcta agcaaacact
    10861 actaccgatt aattttattc cagcaggaat ttataggagt gattattcat acattaaatt
    10921 tttatctcct atgcttactg aaaatcatgc tgattgcctt cactattatg agcggtatgg
    10981 ctatctaatt ctcttatcat acttattagg aattaccgat cttcatttag aaaatataat
    11041 tgccattaat gacactcctt accttttaga tttggaaacg atcttccaaa acaaagtcgt
    11101 tgattttaac gatgccgttt caaacttatt ttacgatcta aatgccgaaa gtatcaatgc
    11161 atcctgtcta ttaccaacaa agttgatggt aaatgagcat gaaagtatcg agcttagtgc
    11221 ctttaaaggt aaaggtacta agcttaagtc ctcatttctt ggccctgtcg atcttgataa
    11281 tcttaatttt cattttacta agaaaaaagg tagttatttt gcaggtggtg ataatatccc
    11341 taaagatacg aatggaaatg aaatcaatta tcttaaatat cgtcatgcca ttatttgggg
    11401 atttaacaaa atggctaact tcatattttc caatcgcaat aatttcatta acgagattaa
    11461 aaaatttagc gaatttcaaa ttcgtatctt aaccaaaagt acggaacgtt atgcttctat
    11521 gttgagatat gctaaccacc ctaattacaa tcaccaaatg caatatcgtg aaaaattgct
    11581 atttaacacc tgggtatatc cataccacga taaatcgatc atcaaaagcg aagttaaaga
    11641 cttgatttat aatgatatac cttttttcta tactcaagtt aaaagcacta gtatttttgg
    11701 cagtgatggg acagagtacc ccaaatattt tgaaaaatct gggttacaac tagcaattaa
    11761 tcggatccat aacttttcag aagtagaata tgaaaatcag aaaactattc tctatgatgc
    11821 gctagacatg accgatgaat taatctgcca ctataccaac caaataaagg aaactgaaaa
    11881 tgatcaattt gattacatca gcatcgctga aagaatagct catcaaatta tggatttagc
    11941 ttataaagac gatcagaatt gcatattaaa aaccttcgac tattctcctg atcatggttg
    12001 gcgaatttat cctaccaacg aaaatcttta ttgtggacta agtgggattt caattttctt
    12061 cattcacctt taccaacata ccgaaaaacg agtctaccaa gatttcttta acaactccct
    12121 acatcaagct attgaatttg caaacctgca acccattaaa tcagcatatt ctggattctt
    12181 ggctcctctg tacccactat tattaatcgc cactaaatca agaagctact caagtcaaga
    12241 aatacattat cttaatcaac tagaatccaa actaaattcg tatatcgaga caattaatga
    12301 tcaaactaaa ttggatttta tgggaggtct aggtggtgtg gtctatctat taaatctaca
    12361 aaaacaagtt tggaatgatc tttatgtttc aaatatgact ttaaaaagac tgaataatat
    12421 tttctcaaaa aatgttatta aatttacaaa cgtctttggc tttgcccacg gatttagcgg
    12481 tctatcatta ccactatatc taatgggtag gcaagattta gccataaaac tactaaaaaa
    12541 agaagccaaa ttagatgaac cacaaatttt cgatcaggca aaatggtgtc gagggatacc
    12601 cggcatatta atggtaaaag cattgttaaa tcagcaaatt gatctcatgg tacctgctga
    12661 agcttttgaa aagattgatg atactttatg ccatggtaaa tgtggattaa tatcttctta
    12721 tctttatttg tcgcgtaaat taaacaacca atacttttat aactatgctt ttataatggc
    12781 aaacgcgatg ctatcagact atttaagtgg taacgagttt aaattaaagc taacaagtaa
    12841 ttcattccag cctggactgt atgatggact atctggaatt ggtttaacaa tattgagttt
    12901 atcctatgat gatattaaaa atcccttaat atttgaactt taattaatga aagggggtga
    12961 ataacatgga tgcaaacaaa aaaattgata ctgtttctgg ctctcctttt gaatctttaa
    13021 gtatcgaaga gatgactaaa atccagggat ctggatcaca aaatatgatg accgtatcgc
    13081 ctgttgctac tcctatagct tctttccaag cttcggaata tatctcacat aaaataaata
    13141 aatttttaca tcgaaattaa agggctaaaa caatggatag taaacaacaa tttaacgata
    13201 cactaggcga agcctttgaa gagttaaaca tcgacgaaat gacaaaaatt caaggaactg
    13261 gctttgaaag tctgtcaatt tcttggccaa ttgtaactta ttttgaaatt tcattatcac
    13321 tagcgcatca agtcaatgtt tattctcatc ttcgtaaaaa gcgcaataaa taactttaga
    13381 taaaggagaa aatcatgagt aaagaagaac taaactcaat agtgggacga gcttttgaag
    13441 aaatggacat tcaacaaatg acgtctattc aaggatccgg aactgaaacc gctcaatcaa
    13501 cacctgcaat ttcgcgagta acactctcca tagcacgtaa gtcatccgca aaatgtattt
    13561 cttggatttc tttttcagca ggtggtctca attcatataa gtcaaaatgt taaaattagc
    13621 cactaaacat atgaggtcaa aaaaatgccg aaaggaaaat taaaaagcaa aattgaactt
    13681 aagaagaaaa tccgtaatac agctattatg tggtttatct ttctatattt ctcgattttg
    13741 gatctctttt atccatcaac ttcttggatt ataacttatt taattccaat cacttgtgtt
    13801 attgaactga tcactctagt ttatttgata tttgaatact tcaaaacaga accatagtca
    13861 aataacctga aaaaccaata aggcgcacca ttggactaac caagaacgaa tgttggaata
    13921 aaaaggacca acgtgagtct aaagttagac ttatggtgca tatttttgcc cgtagttact
    13981 agtgtaatca aaaatcgtgt ttggagtccc ttatacacca ccgtacgtac cattccacac
    14041 acatcagttc aactactttg gtcccctgtc aataaaatag acaatttaaa cagagacttt
    14101 ttgtctatgc tgcggttaag ttttgaatta gagtttggta ctcatcttca aactgctttg
    14161 gtgatttaaa ctcacaacga ctgtgagttc tcaccgtgtt gtaaaatgtt tcaatgtact
    14221 gaaaaactag ccgttttgct tctgagtatg aatggatctt aaaacgattt aaccatttac
    14281 gcttaattag ggcatgaaac gactctatgc aagcattatc cccaggatac gacttctttg
    14341 aataacttaa tgtcatttct gctgttgctt ggttgtatgc ttcagacgtg aactgactgc
    14401 cacggtcact atgcatgatt aatggttggt caatatgacg attttgcttg gccttttcaa
    14461 ctaatggaac aaaaagccga atgacgaact catggttcgt cattcggctt tttgtgtggg
    14521 ggataggaat tttttcagcc taattttgat tatttctcgc ttaacaaaat ccttttaatt
    14581 gccggtattt tccttaaact aaccttacaa aacgccattt cgtccgaaga cgtcaaaaag
    14641 taaactcttc gctctttaac atttaatgag ataacgttat ctaaattaat caatatttgt
    14701 cgatcaaccc gaataaaatt atttcgtttt gcttcaatcg attttaactc accataaaat
    14761 tcaaccgttc gatctttaca tactaattcc aacttatgct gtttattagt tgtcttaata
    14821 tataagattt catcaaccgg aactcgtttt ataagtccta taaatggctc ataagtaaac
    14881 atatttccca tattatgaga cgtaactaat tttgtatagt taaaatatac taaatcaata
    14941 gctctgcgta gttgatgaat catttgttct attccttgcg atttataaat aaaatcaatt
    15001 ggcttagtat ttgaattaat tgttttaaga agaaaatctt cgtatgccga aacatagcaa
    15061 atttccgtaa atccattcgt tttacgtaat tcttcagcaa tttcaattcc agaaacatct
    15121 gagtttaaat caatatctag tactccaaaa taatcttcaa acttattaca acgtatataa
    15181 tcaatcgttt catatgggtc tgacgtacac aaagctatat gcatatcata tgcttttaac
    15241 gtcggattta ttgcgatacg atttaagatc actgttctca taatctctaa ttgctcaaga
    15301 ttattttcta ctagtaacac attaattgtc atctggatcc cttctgatca tataatcaaa
    15361 ctgaattcta tctttatatg tttcgatttc taaagcataa ttatcagaac tatcaatgat
    15421 atctttaata atagttaaac caattccaga atgaccagtt ttagttgaat attcattttg
    15481 aaacaattta tgattgtcaa tttcatgatc aattgtattt acaactttaa aatccaatat
    15541 ttctgagccg tactgattta attctagttc tattctagac tgatatttat ccctagtttc
    15601 ttcaattgca ttatccagca atgagccaat tacagtgact acatcaatcg cattttcttc
    15661 actaatagga aaatcggtat taactgaaag gttacaagaa atgttgtatt cgtgcattaa
    15721 ttgtactttt gaaaaaatta aagatcttaa ttccacagaa ctaagctcat taagtatttg
    15781 atattgacga ttgatttcac tattaatact ttgattaaat tctttaagaa tattattaag
    15841 atagccttta gcagcaagat aatccccatt ttcaagtaac ccatctaaag aaattagttg
    15901 gttaacgtat ttatgccgca acttccttaa tctaagatag tcacgacgat tgaaagctag
    15961 atactcctgg ctttgcgaaa ctccccgttt tacatattca agttgattga cgcgattata
    16021 atacttactt agggatatag taaaacctaa tactattaaa atgataatca ttgaaattag
    16081 catcaatcca ccttcaattc tcatctcaac acctagttct tcggcaatga tctgaaggct
    16141 caatataaaa atataagcta gtaaaattga acattggaaa aattttgtta gcttatgtct
    16201 atataagtaa attcgcagtt ttgataatcc tatttggaca taacgacgat aaaaatataa
    16261 aattactagt actaccgatt cggataccaa ttggcaagta attaatttat taaccgatgc
    16321 attaggaatc aaaacgaaat taaataaaaa gctgatacaa ccaccaaccg ttaacgaaaa
    16381 aattgcacca atgtacacaa tcccattgtt aaagcacaac aacgattctt ttttcattaa
    16441 tctgttatat aaaaccacaa acaatgtata aactagataa ataaaatttc caaaaacccc
    16501 taaacataga acggttaaaa ctactcctcc aaaaaatatt tttttctctt ttattgagct
    16561 attaagcact gaaaaaaaat aacatgatgc cagagacata atttccatca aaaaatactg
    16621 aaccacccca accataatca tttagattca ccacttatga aattttttag ataaagcgtg
    16681 tctaaagata ctatatccat gtactaattt ggtttgtatt tgtccaccaa taatcttatt
    16741 tagctcatcc tcggatattt ttttatagtt tgatttatga ttttctaata tcattaccaa
    16801 acctcccaat tctatattat ttattataat actaacgacc gagattaaaa tatataaaat
    16861 attaatcgct ttaaatttcg attaaatgaa aaagtaggct caatacctac ttcagattgt
    16921 atttgtcatc tagaaatttt tgaaatgaat cataatttat taaaccactt ttcacccaat
    16981 aggtagattc ctcttcagca aatgaatttg gaacatcaga acctattcga tcattcatga
    17041 ttaatttcat tcccttgaat acttcttcaa tataataatt tgattgtagt gaattcccaa
    17101 ttgaacaaat gattttcgac ctaattttat ttgtgacatc attaagccaa acttcatcat
    17161 gtaattgaaa tccattggcg ttacaattag cgataatatt tccacccatt gtactgataa
    17221 ctttacaaag ataactggta acagttgtat caccatttgt actgcatgta cttaaaacaa
    17281 ccacaggttt tccatccaac cttaagatat gagaccaagt agatagtttc tcaattacca
    17341 gttttaaatt tgatgaaata ttgtgcatat aaactggcga agcaataata agtagattac
    17401 tttctaatat tgattcttgt aaagaataaa tattatcacc cgctactttg cgactgtatt
    17461 caggatcata caatccttta taaactaacg gaaacatata atcacttgcg aaaaaaaatt
    17521 gtcgatcaat ttctttagga attcccctca atatttcttc aataaagtat tgagtatttc
    17581 ctttgcgatt aacgcttccg tttaaaactg taatccttct cattatcaaa aattattttt
    17641 tctaatgtga caaacaattc taaataacaa tttgcagctt tctctaataa ctacataaca
    17701 cacggcataa aatgcaattg tcccaaaaat acgggaataa gttgagatca aaataagcca
    17761 actaatacat accattacgg taatatcaag cgtactttct tttttcataa tatcatcgcc
    17821 ttaatattca atatcaagat tctctattta tgatgtttag cccagctaag ccctgagatt
    17881 gccgaaaccc attttgcaca ccttactgaa gatgccttag atataggaat aagtggtgat
    17941 gtctgggcac tttcagctcc attcccctga attgatgtca tctgttgcac actcatatcc
    18001 tcaaatgagt ctccgactat cttttttaat tcgctatcta gcatattaac catctcctta
    18061 tttctatttt gaatgtttaa aagctcgaga tatagcatag gataaggcaa aactctctgc
    18121 aaggctttca acaacaggtg tagattgtgc agactgagtt ccattacctt gaatctttgt
    18181 catttcatca atagtaagtt cttcaaacgg atcacctaca acggtattaa tatctttttc
    18241 cattttactt tcctcctttc tcatagtaca tcttattatt tttaaacaat tatcaatcac
    18301 tttttattaa gcgtttattt tgactaacaa acgcaaaata tcagataaat actgctataa
    18361 ctattacgat cttaatcgca caaaatcgta atatcttttg tgttgagcca ttaattgatt
    18421 atgacttcct ctttcgacaa ctgttccgtt ttcgataaca acaatatcgt caacttgctt
    18481 agctactttc aatcgatgaa ctataaagat aatcgtttta tcttttaaat ttaatagatt
    18541 attaataagt tccctttccg taatcgtatc taaactacta gttgattcat ctaaaattaa
    18601 agtttgagca ttagataaaa tagccctagc tattgttaat cgttgtttct gtcctcctga
    18661 caaaattgta gcttcctcgt cgagttttgt ttcgtatcct aaaggtagcg cgttaatagt
    18721 ttcactaatc atggcaattc ggcaggcttt ctcaactttt tcccggtcta ttttatcgct
    18781 acaacctaag gttaaattat tccaaatcgt atcagaaatt atataagaat actgtggaac
    18841 ataattaatc tccgatcgta acaaagaacg agaaattttc tcaatattga ttccatttaa
    18901 tgtaattgat ccttgtgagg cagttaaaaa acctactaat agcttagcaa tagtcgattt
    18961 ccccgatcca cttgtgccaa ctattgcgat cttattcccc ttttttattt ctaaatcaat
    19021 accttttaag accaaatctt gataaccata gcgacattgc acaccactta aaactatgtc
    19081 accactgata tcatttgaaa ctgtttttaa ttgaatttct ttttcgactg gaactattaa
    19141 aatatcgttt aatcgtctag ttgcaacaat cgcactttgt aaatttgatt ggagatctaa
    19201 gatgtttttt aatgagttcg taaaatatcc taacaaagaa gtaaaagcaa tcaactgtcc
    19261 caatttaaaa tagttattca taatttgatt tgccccaatc caaataacca aaatatttaa
    19321 tgttaattgc aaaaactgct ttattgattc ttgacttgaa gttaatatac tatatttttt
    19381 taactcattt aaaaaatcat taaaggcaag cttgatttta tctaattgtt ttacattcgc
    19441 atttaaacac ttcagcgttt ccattccctt aatgctatca atgattaaag aacttaattt
    19501 agcatttttt atcattacct ttttgttcat tttttcaaag atactagcaa atgtaacgat
    19561 agtaacggtg taaattggta taaacaagcc gatgataagg agtagtcgaa agtttaacaa
    19621 tcccaataca taccccgtta tcatcacaat taacacatct aaaaatatcg taacaattga
    19681 tctagaaagc gcctcaatta ctcgattagt gtcgttgaat ctagacacca catcaccagt
    19741 tcgtcgagtt gcaaaaaaat caataggtaa atcaaataga tgcttgataa agtttaatgc
    19801 aatatctttt gataatcgtt gtcctaaaat tgtcattgct atgctttcaa caaacgaaaa
    19861 tgtagcatta aaaaaataca tacttatcag accaattact aaaaaagtta gtttattaaa
    19921 agcttgtcca ggaattaagc gatcaatcac tagttgaatt gcaagtgaat tgataatatt
    19981 cgtcaataag acaagggctg ccattagaac tattccacaa gtcacaaatc tattttttgt
    20041 tagatacctt atatattcaa agattcctgc cgatttttca ttaatggatt gataatcatc
    20101 tcctttagaa aagagaatcg catttccact ccattcttca cagacctttt tgattgacag
    20161 cctttttatt cccacagaat catcaggatc cgcaactgta atttgatcgc cattaatttc
    20221 ggtaaccaca caataatgta agtaactttc ttttctaatc acatgaatga taaacggtag
    20281 tgggagatga tttatttcaa aaagttgccg attagctttg attgctaatg tatccaaacc
    20341 atatattttg gcagcttgca caattccgta aatggttgtc ccatcgattg ttgttttagt
    20401 acttgtcctt aatttagcga tcgaaacatt agttccaaaa tttttcagta ccatagataa
    20461 ggctgctact ccacaatcct cttcatccat ttggacagta tagtatttat atcgcatgaa
    20521 aattctcctt atttttaatt atatgatcta tccctcctat caatatttat ttgaataagc
    20581 gcctaattta atgactaaac gttaaaattt gtcctataaa aaaactggaa agtaaccaaa
    20641 ttaagtttct ttccaggttg ttttccaact atgatttgta gattcgtttt aagcgcgggc
    20701 tcaaactagt taatatctca taattaatta tgcccgtttt ttcagctaat tccgtagccg
    20761 taatttcatc attaccatcg cgacccagta gcgtcacctt cgtcccgact ggcagttctt
    20821 gtggcaaccg aaccatcatt tggtccatgc acactcgacc aactaagtca caacgttgac
    20881 cgttgatcag aacttgataa ccggacaatt gacgcgtata accatccgca taccccacgg
    20941 gaattgttcc gatccattca tcttgcttag ccgtgtaagt agccccataa ctaactgaac
    21001 gaccggcttt tagttgcttt acaaaagtca atcgagccgt aaccgctaaa gctggctgca
    21061 aatccaatgt tgccggtaag accgtccctg atggattcaa gccatacatc gagatcccca
    21121 tccggacagt atttccggta atctgatcag gatgccataa gccggtcgcg gagtttgcca
    21181 tatggacata tggtggtaaa gtcggtaaaa ctgctaataa ttcatgccaa cgggctattt
    21241 gttgcttaaa gtaagcatca ttagcttcat cagccgttgc aaaatgggtg aaacagcctt
    21301 cgaattcaaa aactggatct ttgagtaatt caagcgcctc ttttaataaa gctggctccg
    21361 taaaaccaat tcgccccatc ccagtatcaa aacctaaatg aatttttaaa ggtttaggaa
    21421 tggaaacagc atgcgctaat tgctgatagg cttttagcca atctacgtcc ccaacagtta
    21481 gtgatatatc attttcagcc gcagtccccg catattgcac cggcgtaatc cccaaaacaa
    21541 gaattaattc ggtcaaccca gcttgccgaa gttctaatcc ttcatctaac gcagccacac
    21601 aaaaaccatc gatgccggct gatgaaagat cttgggccac tgcaacggct ccatgcccat
    21661 aggcattggc cttaactacc ccaaaaatgc ggttgccggc tggcatggct tgtcgttggg
    21721 ctgccacatt atgacgaatt gctgctaggt ttacttcaac ttgagcatct cgtaaattag
    21781 ctgttaccat ctgaaccttc ctccaaaatc acttctgtca tcactagttg atctgtatga
    21841 ctaacgctgg cgtgggcccg tccagaaaaa atcgattgaa aaacttctgg tcgacctaaa
    21901 tcattattaa taaacgaaat atcttggaaa ccaacttggg ttccgattcc tgtccccatt
    21961 gctttagaaa acgattcttt taaagaaaag cgactgctga tatattccca atatcgctgg
    22021 ccgcttaggt tttcgagctg ggcttgttcg gccggagtta atactttagg aataaattgg
    22081 gcatgtttat taactgctgc ttgtacccgt ttaatctcag taatatcaat tccgatccct
    22141 ttgatcatga ttagtcctct tttcgccgaa tcttaaatga attacgtttt ccctgactcc
    22201 gtggattccg atgactacca ttaccaccac gtggacgtcg attccgattg tcacctcgac
    22261 ggttaccacc attggaccgg cgcctgtagt gattaccacc attccggtta ggtttccgtt
    22321 tccgatttgg taatggtcgt tctggagtga tcttgaccgg cacttgggtc tcatcatcct
    22381 tcgttaactc atttaataag gttgccacca agtccaccgc ttcatgagta gcgagtagtt
    22441 cactcgctgc ttcacgataa cgatcgttgt ccggacgttc aatcagttcg ttaatatcat
    22501 taaaggcact ggccacctgt ccccgaaaag cttcttcagc cgttggtggt tttaaaggca
    22561 acatccgcac cttcgtcaac ttttcaattt catggagata gtccatttca tttggcgtca
    22621 caaaagttag tgagacacca tggttgccgg cccggcctgt ccgaccaatc cggtgaacat
    22681 aactttcggg atcttgtgga atatcatagt tgtatacatg cgtaactcca gaaatatcca
    22741 agccccgggc cgctacatct gtggcgacta gaatatctag ttcgccattc ttgaatcgtt
    22801 tcatgatctt ggagcgccga tcttgtgtca aatcaccatg aattccggca gcattgtagc
    22861 cccgagcaat taaaccacgc gacaattcat ccacccgccg cttcgttcga ccaaaaacaa
    22921 tggtcaggtc aggatcttgg acatcaatta accgcgtcat gatatcaaat ttttcgtaat
    22981 cacgggcctt aatgtagtat tgatcaacta agtccgtagt taattccttt gccttaatct
    23041 taaccgtttg tgggtccttc ataaattgga ccccaatccg tttaatttcg ggtggcattg
    23101 ttgctgagaa caagagggtt tgacgatctg atggtgttgc ctgaatgatt gcctcaatat
    23161 catccaagaa gcccatattt aacatctcat cggcttcatc caagactaaa gtttgcaact
    23221 gatccaattt gatcgttccc cgattcaaat gatcacgcaa acggccgggg gtcccaacaa
    23281 taatttgggg accatttttt aaagctttaa tttgccgccg aatatcagcg cccccgtaga
    23341 caacttggac acgggcccgt ttttcctttc ccaatttata taattcttct tgggtttgaa
    23401 tcgctaattc ccgggttggc gaaatgatca acgcttgaat tgctgatgat tgcgtatcaa
    23461 catgctctaa aactggcaac ccaaaggccg ccgtcttccc agtcccggtt tgagcttggc
    23521 cgatcacatc gagtcccttc aaaaccatcg gaatggtttg ctcttgaatc ggggtggctt
    23581 cttcataacc acttcgttga accgctttta ataacttatt tgataaacct aattcactaa
    23641 aatacaaatt tctacctcat tatcctttcg tgagtccttg caggaccgtt tccaaatgaa
    23701 tgccatggct ccctttaata agacaatatc atctgtcctt aaggtggcgt tcagatcctt
    23761 cgtcaattgt gctaatgcat tcgtgtcata gtggtgtaac cgtttgttag ggaaaccatc
    23821 ggccgtcaat tgtttcaata aagcttgcat ttcatccccg acgagataaa cttccgcaat
    23881 ctgatcagaa tcaatctctg ccgctaattg agcatgaagt tgtgcggagg ctgtccccag
    23941 ctccaacatg tcgccaagca caattacttt tttaccctcg gtttgaactt gctcaatcgt
    24001 ctttaagact tcggccacgg cggtcggatt agaattatag acatcactta gaatccgttc
    24061 gccattggtt ccttgaatcc attcagcccg attttcagtc atctgcagac cttgtaaggc
    24121 cttttgcatg ttttcaggag taatctggag ttgagcacca actgcaatcg cggctagtgc
    24181 attattaacg ttatactccc ccaccattgg aagggtaaaa gtaaggtcag gccattcatt
    24241 cacactaaag gcaacttgat catcagccaa ttgaacacta gtggcataaa aatcatcggc
    24301 taactgtcgc ccaaaggtca caacttgttg ctcaatatcc tcagcacgtg ccgttaataa
    24361 tggttcatct ccattaataa ttagggttcc atcttcgttc atcccgtgaa cgatctccat
    24421 cttcgcatcc gcgattcgat cacgcgttcc aaagaattca atatgtgctt ccccaatcat
    24481 cgtaataacc gcatagtcag gggtggctaa ccgacttaat aggtctaatt gatcgaagcg
    24541 atccatcccc atttccacga caaccacttc agtcgatggt tccatattta acagggttac
    24601 tggaaccccg atttcattat taaagttggc aaaggttttc gtgacattga actgggtcgc
    24661 taaaatggcg gctaccatgt ccttggtggt cgtttttcca ttacttccag ttaccgctac
    24721 tacaatcggg ttaatctttt ttaagtaata ctgactgagg cgctgaaagg ccgctaatgg
    24781 gtctttcact actagtcgag ggaattggtc atcgctcgga atatcatggt cagctgccca
    24841 caaagtcgct tttgcaccat tttgaaaggc ttgtgccacg aattcgtgac catcccgtgc
    24901 cccttgtaag ggcacaaata aagacccagg agtcaaatta cggctgtcaa aagccacgct
    24961 ggtaacttcc aactcatccc aggctgcatt tatatcttga gcatcaattg ccttagcaat
    25021 ttccgctaat ttcattttca tgagtttgca ctcctcaatt tcgtcatgtt tgtccaactt
    25081 ttaattatac actgttcacg aaaacataga tagcaccaac ataaaaacag gctaggcaag
    25141 aaaatatcct gtctaacctg ttttaggtac ccgtcatgaa gattatcagc ttctcaattt
    25201 acattttctc taaccgcgca atccgatctt ctaaaggtgg atgcgtatca aatagatggc
    25261 taaaagaacg atgtttttga ttcttaaagg gatcactaat atataaggcc gagctattgg
    25321 gatcggcagc cttcatcggt tgtacatcct ctaattttct tagcgcacta atcattccct
    25381 gagggttacg cgttaactca acagcactag catccgctaa atattctcga ttccgcgaca
    25441 gcgccatttg agcgatcgaa gccgccagtg gtgctaagat gattagcaat accgaaccca
    25501 agatagcgat aacccca
//
