LOCUS       NZ_CP045572            10936 bp    DNA     circular CON 07-NOV-2024
DEFINITION  Nonomuraea phyllanthi strain WYY166 chromosome, complete genome.
ACCESSION   NZ_CP045572
VERSION     NZ_CP045572.1
KEYWORDS    RefSeq.
SOURCE      Nonomuraea phyllanthi
  ORGANISM  Nonomuraea phyllanthi
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 11273186)
  AUTHORS   Ou,Y. and Wu,Y.
  TITLE     Nonomuraea nitratireducens sp. nov., a new actinobacterium isolated
            from Suaeda australis Moq. rhizosphere
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11273186)
  AUTHORS   Ou,Y. and Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2019) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Dongchuan, Shanghai 200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Aug-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: PacBio RSII; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009497075.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 06:36:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,403
            CDSs (total)                      :: 10,325
            Genes (coding)                    :: 10,209
            CDSs (with protein)               :: 10,209
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 22 of 116
            Pseudo Genes (incomplete)         :: 104 of 116
            Pseudo Genes (internal stop)      :: 8 of 116
            Pseudo Genes (multiple problems)  :: 13 of 116
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:05:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9773525
            Orig. end                         :: 9784461
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="27"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(214..225)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    659..674
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.04"
                     /tool="antismash"
     gene            1194..1679
                     /locus_tag="GBF35_RS44870"
                     /old_locus_tag="GBF35_44840"
     CDS             1194..1679
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBF35_RS44870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBF35_44840"
                     /product="hypothetical protein"
                     /protein_id="WP_139636044.1"
                     /transl_table=11
                     /translation="MAALCVLAAAACGIAPTDVQDRGSAPTISPPPPSKTIYLLRNGKL
                     ALEPADVENDTVDSLLGALFAASSKPLGDRDTALRGFTYLGIKDSLNPVQRDEVQLPRT
                     STLTVYIRGEGILTKLGKAQIVCTAQQNIAFEQVKIVRRNAHHPSKSEGQHTCPELR"
     gene            complement(1684..3003)
                     /locus_tag="GBF35_RS44875"
                     /old_locus_tag="GBF35_44845"
     CDS             complement(1684..3003)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133320.1"
                     /locus_tag="GBF35_RS44875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44845"
                     /product="PhoH family protein"
                     /protein_id="WP_263008701.1"
                     /transl_table=11
                     /translation="MAVSSSNPTNQPVKRTYVLDTSVLLADPAAMTRFAEHDVVLPIVV
                     ITELEGKRHHPELGYFARKALRYLDELRMQYGRLDEPMPTGDGTIRVELNHSDPSILPV
                     GFRLGDNDTRILTVARSLAAEGCDVVLVSKDLPLRVKAGSIGLAAEEYRAELVHESGWT
                     GMAEIQVTAEGVEVLFDQGTADLDEARDLPAHTGLRLLSAKGSALGRVQPDKSVRLVRG
                     DREVFGLHGRSAEQRIALDLLMDPEIGIVSLGGRAGTGKSALALCAGLEAVLERRQHRK
                     VVVFRPLYAVGGQELGYLPGTEGEKMGPWAQAVYDTLGAITSPEVIEEVLDRGMLEVLP
                     LTHIRGRSLHDAFVIVDEAQSLERGVLLTVLSRIGANSRVVLTHDIAQRDNLRVGRHDG
                     VVAVVEKLKGHPLFAHVTLTRSERSPIAALVTEMLEDITL"
     misc_feature    2963..2978
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.13"
                     /tool="antismash"
     gene            complement(3210..3971)
                     /locus_tag="GBF35_RS44880"
                     /old_locus_tag="GBF35_44850"
     CDS             complement(3210..3971)
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545137.1"
                     /locus_tag="GBF35_RS44880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44850"
                     /product="isoprenyl transferase"
                     /protein_id="WP_139636042.1"
                     /transl_table=11
                     /translation="MGLRDLVYRLYEHRLESKLSKDGIPRHVGVIIDGNRRWARAMGMR
                     DVASGHRKGADKISELLGWCRETGVEVVTVWMLSNDNLNRPKEELEPLLRIIEDVTQEL
                     VDEGWRISPVGALDLLPDRTANVLKNAKEASSHRPGLIVNVAVGYGGRREIADAVRSLL
                     QEHASKGASIEDLVEVLDVEHIAEHLYTRGQPDPDLLIRTSGEQRLSGFMLWQSAHSEF
                     YFHEAYWPDFRRVDFLRALRDYAARHRRYGS"
     misc_feature    3934..3950
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            complement(4024..4524)
                     /locus_tag="GBF35_RS44885"
                     /old_locus_tag="GBF35_44855"
     CDS             complement(4024..4524)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545138.1"
                     /locus_tag="GBF35_RS44885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44855"
                     /product="hypothetical protein"
                     /protein_id="WP_228010686.1"
                     /transl_table=11
                     /translation="MACKDSVGTPYEVTLELHRDGTPYGSVGERCGWLLARLARGVDDA
                     REGRGTARRWADPDDRFPDEEPDSELFSFRYRTRAGLVGGGELRCQLRTIPVWQAARGE
                     WRLTRRAYVEAWGMTGVGMRAVLTSAELSVFVRGLVDEAEGCLEGRDLARNPIDGAGTG
                     ESR"
     gene            complement(4636..5004)
                     /locus_tag="GBF35_RS44890"
                     /old_locus_tag="GBF35_44860"
     CDS             complement(4636..5004)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBF35_RS44890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBF35_44860"
                     /product="hypothetical protein"
                     /protein_id="WP_153264932.1"
                     /transl_table=11
                     /translation="MTHGHDVGEARRRLAEAQGRVVAALVAGAAVPEGFDPERMRVQAA
                     SLVAKRRSVVARLRPDAAAAAGPDLAAEFAAYARSREEPPPGYRADADDFAAWLRERGR
                     LRDPAPEPRRRWWSRFLR"
     gene            complement(5001..5936)
                     /locus_tag="GBF35_RS44895"
                     /old_locus_tag="GBF35_44865"
     CDS             complement(5001..5936)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="GBF35_RS44895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44865"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_153264933.1"
                     /sec_met_domain="DUF692 (E-value: 1e-81, bitscore: 273.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEQEAGRPAAPNGGPGAGPNGGSNGGPVGGPVGGPGGGSRGELG
                     VGIGWRAELDLSIERMPGVDFLEVVAENVRPDHLPESLRLLRARGLPVIPHGVSLGVGG
                     AEPPSPERLAHLASCAQALESPLVSEHLAFVRGGGLEAGHLLPVPRTRESLRVVTENVR
                     RAQDALPVPLALENVAAIFGWPDDELTEAQFLAELVERTGVGLLVDVANLYTNQVNLGL
                     PAVAALDALPLERLAYVHVAGGYEHAGIWHDTHTAFAPQEVLDLLTELCARATPPGVLL
                     EWDDDYPSDAALESELARIRSAMSAARDLR"
     misc_feature    complement(5499..5501)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5688..5690)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5938..6876)
                     /locus_tag="GBF35_RS44900"
                     /old_locus_tag="GBF35_44870"
     CDS             complement(5938..6876)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="GBF35_RS44900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44870"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_194244039.1"
                     /transl_table=11
                     /translation="MDLVLLIVSVALAALAVTIASMIKREHAAVRAAADGIRARDLAHY
                     ELAYLAGGAEQVVGTAITLLAERGDLRVSRGPRFHAVSRRSTTDDPIEQATLAAVAGNG
                     GPSRWLVKQAAGDSQAMKEFQGRLAGLGLILSDARLARLRRLATGLGVVSVLAAAGAVA
                     GILAGAVFADPALLPLLAALLMLATAIGGAIVLADDRRSRRGALTSYGRAELDRARKAY
                     PVDSGHEPVHVALYGHWVLRDAELVRSLDTYVPPARPRRGRRSSSSGGGGGGGYYGGCG
                     GAAAGGSGCGSGSSGCGGGSGCGGGGCGGGS"
     gene            complement(6993..7976)
                     /locus_tag="GBF35_RS44905"
                     /old_locus_tag="GBF35_44875"
     CDS             complement(6993..7976)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="GBF35_RS44905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44875"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_139636033.1"
                     /transl_table=11
                     /translation="MDYVLIVIAFGVFFLVNAANDAVERERRRVAEAAPSGNAHHLSPY
                     ELAYLSGGPRRVINTALAVLATAGAVRVSRGSQVTPVHGARPSPVPIEQAVLDTLAARP
                     GGYRAGELRRELETHPALAALAGGLLSRGLIVPEEAYGPARRRRNVLKAVVGLAVAYLV
                     LLFALDAAGVAGEEPVIIGALLFTAFAAVVGVVAVVRQRRRLRNVVTREGRGVLRSAYG
                     HHPRGVRDQSSLALAVGIPVALYGLGDAGDQTLCDELSAGDPGGDCAGSCGTHSGGSDS
                     FGGHDFGGLDTGGGSSGGCGSSSCGGGSSSCGSSCGGGGSSCGGGT"
     misc_feature    7942..7957
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
     gene            complement(8044..9837)
                     /locus_tag="GBF35_RS44910"
                     /old_locus_tag="GBF35_44880"
     CDS             complement(8044..9837)
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="GBF35_RS44910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44880"
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_153264935.1"
                     /transl_table=11
                     /translation="MSVSVEVPAPTSNRELAAWVKEIAALTQPDRIEWCDGSEEEWTRL
                     TNLLVEQGTFTRLAARPNSFYAKSDPGDVARVEDRTFICSEREEDAGPTNNWVAPDEMR
                     RTFGTLFKGCMRGRTMYVVPFCMGPLGGEISQLGVEITDSPYVAVSMRVMTRMGDRALR
                     RIEERGSFVKAVHSVGAPLEPGQEDVPWPCNPTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASAMARDEGWLAEHMLILKLTPPSGETRYIAAAFPSACGKTNLAMLQPTIP
                     GWKVETIGDDIAWMRFGDDGRLYAINPEAGFFGVAPGTGEVTNANAIRTLWGNSIFTNV
                     ALTDDGDVWWEGLTEKPPAHLTDWKGRSWTPESGEPAAHPNARFTTPASQCPTIAPEWQ
                     DPKGVPISAILFGGRRATAVPLVTESLSWEHGVFLGANVASEKTAAAEGKVGELRYDPF
                     AMLPFCGYNMGDYFGHWLDVGRRAGAKLPRIYYVNWFRKNADGKFIWPGFGENSRVLKW
                     IVDRLNGEAKAVPTPIGLLPAELDTEGLDLTDEDLRTLLSVDREAWREEASLIPAHFDK
                     FGSHLPKELWDEYNALLDRLG"
     misc_feature    9901..9916
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.8"
                     /tool="antismash"
     misc_feature    9980..9993
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.32"
                     /tool="antismash"
     misc_feature    9981..9994
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.28"
                     /tool="antismash"
     misc_feature    complement(9982..9995)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.58"
                     /tool="antismash"
     misc_feature    complement(10003..10018)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.86"
                     /tool="antismash"
     misc_feature    complement(10022..10037)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.39"
                     /tool="antismash"
     misc_feature    complement(10044..10059)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.47"
                     /tool="antismash"
     gene            complement(10086..10607)
                     /locus_tag="GBF35_RS44915"
                     /old_locus_tag="GBF35_44885"
     CDS             complement(10086..10607)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851443.1"
                     /locus_tag="GBF35_RS44915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_44885"
                     /product="hypothetical protein"
                     /protein_id="WP_153264936.1"
                     /transl_table=11
                     /translation="MATDDETARIHGWFAGRLPEGWFTGAPEIVRDREEIAVLGTLPDP
                     PGDVPEAERAALIEGLARRFREETRERRIEIAGEAEHKFRQKVSWGVVCGGETVMFTTL
                     SVPVMTRLRQGERQVLDTLVAAGVARSRSDALAWCVRLVGRHTDTWLADLRDALQHVDR
                     VRSAGPDVNS"
     misc_feature    10607..10620
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.35"
                     /tool="antismash"
     misc_feature    complement(10609..10622)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.42"
                     /tool="antismash"
ORIGIN
        1 aacgggccgg gcaggagcgt gctgatcgag tacggcaaca tgatctccgc gcggggcccc
       61 atggccatga gcgacgtgcc caacgagctg tacggcaggg ccaagcagag cctggtcacc
      121 cagcgcaaga tcatccgtga ccagctctgg ctcatcgtcg gcacggaggt ctaccgcgac
      181 accgacggcg cgcccgagcc caccgggctg cgcgcgttcg tgttcttccc gatgcgcgac
      241 gacgtgcgcc agctcggcac gctcaggacc accctcgccc aggccggcgc ggccatcgtg
      301 ctgatcgcgc tggtcgtggc gctgctgtcg gccaggcagg tgctgctgcc ggtcaggcgg
      361 ctgagcgcgg ccgcgcaggc gctcgggcag ggccggctcg acacccgcct gcccgtccac
      421 ggccaggacg agctggccga cctgaccgcc aggttcaacg acgccgccgc ggcgctggag
      481 ctgagcgtgt cggagctgcg ctcgctggag gccatctcgc gccggttcgt ggcggacgtc
      541 tcgcacgagc tgcgcacgcc gctgaccgcg atgacggccg tggccgacgt gctctcggag
      601 gaggccggcc ggctgcccaa ggcgcccgcg gaggccgtac ggctggtgct cagggagatc
      661 gagcgcctgc gcgagctggt cgagcacctc atcgagatca gccgcttcga cgcgggcacg
      721 gccacgctca acgtcgagcc ggtgaacgtc gccgacgcca tcgccgactg cctggaggtg
      781 cgcggctgga gcgaccaggt gcaggtgaag gcgtcgcagg gcctgaccgt gaacctcgac
      841 ccgaggaggt tcgacgtcat cgtggccaac ctggccggca acgcgctcaa gcacgggctg
      901 cccccgatca cggtgagcgc cagggcgacc gagaacggcc tggaggtgaa ggtgcgcgac
      961 cacggcaagg gcatccccgg ggaggcgctg gcgcacgtct tcgaccggtt cttcaaggcc
     1021 ggctcgggcc gggcccgcag ccagggcagc ggcctcggcc tggcgatcgc ccgcgcgaac
     1081 gtccagctcc acggcggcac catctccgta cggccgcagc atcccggcac cctgttcacg
     1141 gtctggttcc cgcactcatg accgcgcgcc gccccctgag agccctccgc catgtggcag
     1201 ccctctgcgt gctcgccgcg gcggcctgcg gcatcgcgcc cacggacgtc caggaccgcg
     1261 gctccgcgcc caccatcagc cccccgccgc cgtccaagac gatctacctc ctgaggaacg
     1321 gcaagctcgc cctggagccg gccgacgtgg agaacgacac ggtggacagc ctgctggggg
     1381 cgctcttcgc ggcgtcctcg aagccgctcg gcgaccggga caccgcgctg cgcggcttca
     1441 cgtacctggg catcaaggac tcgctgaacc cggtgcaacg ggacgaggtc cagctgccgc
     1501 gcacctcgac gctgaccgtc tacatcaggg gcgagggcat cctgaccaag ctgggcaagg
     1561 cgcagatcgt ctgcacggcg cagcagaaca tcgcgttcga gcaggtcaag atcgtacgca
     1621 ggaacgcgca ccacccctcc aagtcggagg ggcagcacac gtgcccggag ctgaggtgag
     1681 ctcctagagc gtgatgtcct ccagcatctc cgtcaccagc gcggcgatcg gggagcgctc
     1741 ggacctggtc agggtcacgt gcgcgaagag cgggtgaccc ttgagcttct ccaccaccgc
     1801 caccacgccg tcgtgccggc cgacccggag gttgtcgcgc tgggcgatgt cgtgggtgag
     1861 caccacccgc gagttggcgc cgatgcggct cagcaccgtg agcagcaccc cgcgctccag
     1921 cgactgggcc tcgtccacga tcacgaacgc gtcgtgcagc gaccggccgc ggatgtgcgt
     1981 cagcggcagc acctccagca tgccgcggtc gagcacctcc tcgatcacct cgggcgaggt
     2041 gatcgcgccc agcgtgtcgt agaccgcctg cgcccacggg cccatcttct cgccctcggt
     2101 gcccggcagg tagccgagct cctggcctcc cacggcgtac agcgggcgga agacgaccac
     2161 cttgcggtgc tggcggcgct ccaggacggc ctccaggccc gcgcagagcg ccagcgccga
     2221 cttgccggtg ccggcccggc cgccgagcga gacgatgccg atctccgggt ccatcagcag
     2281 gtcgagcgcg atgcgctgct cggcggagcg gccgtgcagg ccgaacacct cgcggtcgcc
     2341 gcgcacgagg cgtacggact tgtccggctg cacccggccg agggccgagc ccttggccga
     2401 cagcagccgc aggccggtgt gcgccggcag gtccctggcc tcgtcgaggt cggcggtgcc
     2461 ctggtcgaag agcacctcga cgccctccgc ggtcacctgg atctcggcca tccccgtcca
     2521 gccggactcg tggaccagct cggcgcggta ctcctcggcc gccaggccga tcgagcccgc
     2581 cttgacccgc agcggcaggt ccttggacac gagcacgacg tcgcagccct cggcggccag
     2641 cgagcgggcc acggtcagga tgcgcgtgtc gttgtcgccc aggcggaacc cgacgggcag
     2701 gatggacggg tcgctgtggt tgagctcgac cctgatggtg ccgtcccccg tgggcatcgg
     2761 ctcgtcgagc ctgccgtact gcatccgcag ctcgtcgagg tagcgcaggg ccttcctcgc
     2821 gaagtagccg agctcaggat ggtgccgctt cccctccagc tccgtgatga ccacgatcgg
     2881 aaggacgacg tcgtgctcgg cgaagcgggt cattgccgcc ggatcggcca gcaaaaccga
     2941 cgtgtccagc acgtacgtgc gcttgaccgg ctggttcgta gggttgctcg aggacactgc
     3001 cacacgttct cccccgggca cccataggtg caggtgccct gcagacgagg cgggaatcgg
     3061 accggacccg cttccccgac cgcgagccgc cgtcggcgcc gggacccggc ccccctcggc
     3121 agatcttggt gcaaaggcga gccctctccg gatatccgac ctgtggccgg atatttccta
     3181 cgttacgtcg tgaatgcctg attgtcgcgt caggatccgt aacgtcggtg tctcgcagcg
     3241 taatcgcgca gcgcccgcag gaagtcgacc ctgcggaagt caggccagta ggcctcgtgg
     3301 aagtagaact cggaatgagc gctctgccag agcatgaagc ccgacagacg ctgctcgccc
     3361 gaggtccgga tgagaaggtc cgggtcgggc tggccgcgag tgtagagatg ctccgcgatg
     3421 tgctccacat cgagcacctc gacgaggtcc tcgatgctcg ctcccttgct cgcgtgctcc
     3481 tggagcagtg agcgcaccgc atcggcaatc tcacgccttc ctccataccc aactgcaacg
     3541 ttcacaatca ggcccggacg gtgtgatgac gcttcctttg catttttcag gacattcgcg
     3601 gtgcggtccg gcagcaggtc gagcgccccc accgggctga tccgccatcc ctcgtccacc
     3661 agctcctgcg tcacgtcctc gatgatgcgc agcagcggct ccagctcctc cttgggccgg
     3721 ttgaggttgt cgttggacag catccacacg gtgacgacct cgacgcccgt ctcacggcac
     3781 cagccgagca gctcggagat cttgtcggcg cctttgcggt ggccgctggc cacgtcgcgc
     3841 atgcccatgg cgcgggccca gcggcgattg ccgtcgatga tgaccccgac gtgccggggg
     3901 atgccgtcct tcgacagctt ggactccagc cggtgctcat aaagccggta gaccagatcg
     3961 cgcaggccca cggagttcct ccccagcggt agcccttggt cgctatgacc caacaggcaa
     4021 ttatcaccgc gattcgcccg tgcctgctcc gtctatcgga ttcctggcca gatctctacc
     4081 ttccagacat ccctcggcct catccacgag acctcgcacg aacaccgaca gctccgccga
     4141 ggtcagcacc gcccgcatgc ccacccccgt catgccccac gcctccacgt acgccctcct
     4201 ggtcagccgc cactcgcccc tggccgcctg ccacacggga atggtgcgca gctggcagcg
     4261 gagctcgccg ccgcccacca gaccggccct cgtgcggtag cggaaggaga acagctcgct
     4321 gtccggctcc tcgtcgggaa agcggtcgtc ggggtcggcc caccgccggg ccgtgccccg
     4381 gccctcgcgg gcgtcgtcca cgccgcgcgc caggcgagcg agcagccagc cgcaccgctc
     4441 cccgacgctc ccgtacggcg tgccgtcgcg atgcagctcg agggtgacct cgtacggcgt
     4501 gccgacgctg tccttgcatg ccaccggcgt gacggtcaga tatgaaccgt cgacgcaacg
     4561 cagtcccccc attccctcac ggtttccggg ctaatgtcgg ctaaaccgcc gtatgccgga
     4621 tccgctcagc gccgttcacc gcaggaagcg ggaccaccag cggcgccgcg gctccggcgc
     4681 cggatcccgc aggcgcccgc gttcccgcag ccaggcggcg aagtcgtcgg cgtcggctcg
     4741 gtagccgggc gggggctcct cgcgggagcg cgcgtaggcg gcgaactccg cggccaggtc
     4801 gggccccgcc gcggcggcgg cgtccgggcg gagcctcgcc accacgctgc ggcgcttggc
     4861 caccaggctc gccgcctgca cccgcatccg ctccgggtcg aacccctccg gcacggccgc
     4921 gcccgccacg agggccgcca ccacccgccc ctgcgcctcc gccagccgcc gccgcgcctc
     4981 ccccacgtcg tgcccgtggg tcatcggagg tcccgggcgg cggacatcgc ggacctgatc
     5041 ctggccagct cgctctccag ggccgcgtcg ctcggatagt cgtcgtccca ctccagcagc
     5101 acgcccggcg gggtggcgcg ggcgcacagc tcggtgagca ggtcgagcac ctcctgaggg
     5161 gcgaaggcgg tgtgcgtgtc gtgccagatc ccggcgtgct cgtacccgcc ggcgacgtgc
     5221 acgtacgcga gccgctccag cgggagcgcg tcgagcgcgg ccaccgccgg caggcccagg
     5281 ttgacctggt tggtgtagag gttggccacg tcgaccagca gccccacccc cgtgcgctcc
     5341 accagctcgg cgaggaactg ggcctcggtc agctcgtcgt cgggccagcc gaagatggcg
     5401 gccacgttct ccagggccag ggggacgggc agcgcgtcct gggcccggcg cacgttctcg
     5461 gtgacgacgc gcagggactc gcgggtgcgg ggcaccggta acaggtgccc ggcctccagg
     5521 ccgccgccgc ggacgaacgc caggtgctcg ctgaccagcg gcgactccag cgcctgcgcg
     5581 caggaggcca ggtgggccag ccgttccggg gacggcggct cggcgccgcc gaccccgagc
     5641 gagacgccgt gcgggatcac cggcaggccc ctggcgcgca ggaggcgtaa cgactcgggc
     5701 aggtggtcgg gcctgacgtt ctcggcgacc acttcgagga agtcgacacc gggcatccgc
     5761 tcgatcgaca ggtccagctc ggcccgccag ccgatgccca cccccagctc tcccctcgac
     5821 ccgccgccag gcccaccgac cggcccaccg accggcccgc cgttcgaccc gccgttcggc
     5881 ccggcgcccg gcccgccgtt cggtgcggcc ggccgtccgg cctcctgctc gggcatgtca
     5941 gctccctccg ccgcaaccac cgccgccgca gccgctcccg ccgccgcagc ccgaggagcc
     6001 gctgccgcac cccgacccgc cggcggccgc cccgccgcaa ccaccgtagt agccgccccc
     6061 gccaccgccg ccactggacg acgagcgccg cccgcgccgt ggccgggccg gcgggacata
     6121 ggtgtcgagc gacctgacca gctccgcgtc gcgcagcacc cagtggccgt acagcgcgac
     6181 gtgcaccggc tcgtgccccg agtccacggg gtacgccttc ctggcccggt ccagctccgc
     6241 ccgcccgtac gaggtgagcg cgccccgcct ggacctcctg tcgtccgcga gcacgatcgc
     6301 cccgccgatc gcggtggcca gcatgagcag cgcggcgagc agcgggagca gcgcggggtc
     6361 tgcgaaaacc gcgcccgcca ggatgccggc gacggccccg gcggccgcca gaaccgacac
     6421 cactcccagc ccggtggcca gccgcctgag ccgggcgagc cgggcatcgg acaggatcag
     6481 gcccagcccg gccagccggc cctggaactc cttcatggcc tggctgtcgc ccgccgcctg
     6541 cttcaccagc caccgcgacg ggccgccgtt ccccgcgacg gcggccagtg tcgcctgctc
     6601 gatggggtcg tccgtggtgg atctccggga gacggcgtgg aagcgtggcc cccgggacac
     6661 ccggaggtct cccctttccg ccaggagcgt gatggccgtg ccgaccacct gctcggctcc
     6721 gcccgccaga taggcgagct cgtagtgcgc gaggtcgcga gcgcggattc cgtccgcggc
     6781 cgcccggacg gcggcgtgct cgcgtttgat catggaggcg atggtgacgg cgagcgccgc
     6841 cagggccacc gagacgatca gcaggacaag gtccatttcg gcctccctcc gtacagccgg
     6901 caagggctgg ttaccggaga gacgcgcaag gcggcggcgt agtttcgttc tgctcattta
     6961 gcttccggaa acctgcgtta ccggaaacgg cgtcaggtgc cgccgccgca gctcgatccg
     7021 ccgcctccgc agctggagcc gcagctcgac gagcccccgc cgcagctcga cgacccgcag
     7081 ccgccggacg agccgccgcc cgtgtcgagc ccgccgaagt cgtggccccc gaaggagtcg
     7141 ctcccgccgc tgtgggtccc gcaggagccg gcgcagtcgc cgccggggtc gccggcggac
     7201 agctcgtcgc agagggtctg gtcgcccgcg tcccccagcc cgtacagcgc gacggggatg
     7261 ccgacggcca gggccagcga gctctggtcg cgcacgcccc tcgggtggtg gccgtacgcc
     7321 gagcgcagga ccccccgccc ctcccgggtg acgacgttgc gcagcctgcg ccgctgccgg
     7381 acgacggcga cgacccccac gacggcggcg aaggccgtga agagcagcgc cccgatgatc
     7441 accggttcct cgcccgccac cccggccgcg tccagcgcga acagcagcac caggtaggcg
     7501 accgccaggc ccacgacggc cttgagcacg ttccggcgcc gccgcgcggg gccgtacgcc
     7561 tcctccggca cgatcagccc ccgtgacagg agcccgccgg ccagcgcggc gagcgccgga
     7621 tgcgtctcca gctcccgccg cagctcgccc gcccggtagc cgcccggcct ggcggccagc
     7681 gtgtcgagca cggcctgctc gatgggcacc ggcgagggcc gggcgccgtg gacgggcgtc
     7741 acctgcgagc cgcgcgacac gcggacggcg ccggccgtcg cgaggacggc cagcgccgtg
     7801 ttgatgaccc gccgggggcc gccggagaga tacgccagct cgtacggcga caggtgatga
     7861 gcattgccgc tcggcgccgc ctcggcgacc cgcctgcgct cccgctcgac ggcgtcgttg
     7921 gccgcgttga cgaggaagaa cacgccgaag gcgatgacga tcaggacgta gtccatggct
     7981 gccccctcga agcggtacgg cagtccgcca agttagacgg cgccgccgta ccgcacaagg
     8041 ggctcagccg agccggtcga gcagggcgtt gtactcgtcc cacagctcct tcggcaggtg
     8101 ggagccgaac ttgtcgaagt gggccgggat cagcgacgcc tcctcgcgcc acgcctcccg
     8161 gtcgaccgac agcagcgtgc gcaggtcctc gtcggtcagg tccagcccct cggtgtcgag
     8221 ctcggccggc agcaggccga tcggggtcgg cacggccttg gcctcgccgt tgaggcggtc
     8281 gacgatccac ttgagaacgc ggctgttctc accgaagccc ggccagatga acttcccgtc
     8341 ggcgttcttg cggaaccagt tgacgtagta gatccgcggc agcttggcgc ccgcgcggcg
     8401 gccgacgtcc agccagtggc cgaagtagtc gcccatgttg tagccgcaga acggcagcat
     8461 ggcgaacggg tcgtaccgca gctcgcccac cttgccctcg gcggcggcgg tcttctcgga
     8521 ggcgacgttg gctccgagaa agacgccgtg ctcccagctc agcgactccg tcaccagcgg
     8581 gacggcggtg gcgcggcggc caccgaacag gatcgccgag atgggcacgc ccttggggtc
     8641 ctgccactcg ggtgcgatgg tcgggcactg ggaggccggg gtggtgaagc gggcgttcgg
     8701 gtgggcggcc ggctcgccgc tctccggcgt ccaggaccgg cccttccagt cggtcaggtg
     8761 cgcgggcggc ttctcggtca gcccctccca ccagacgtcg ccgtcgtcgg tgagcgcgac
     8821 gttggtgaag atgctgttgc cccagagcgt cctgatggcg ttggcgttgg tcacctcgcc
     8881 ggtgccgggc gcgacgccga agaagccggc ctccgggttg atcgcgtaga ggcggccgtc
     8941 gtcgccgaag cgcatccagg cgatgtcgtc gccgatcgtc tcgaccttcc agccggggat
     9001 cgtcggctgg agcatggcga ggttggtctt gccgcaggcg ctcgggaagg ccgccgcgat
     9061 gtagcgggtc tcgccggagg gcggggtgag cttgagtatc agcatgtgct cggccagcca
     9121 gccctcgtcg cgggccatgg cgctggcgat gcgcagcgcg tagcacttct tgccgagcag
     9181 ggcgttgccg ccgtagccgg agccgtacga ccagatctcg cgggtctccg ggaagtggct
     9241 gatgtacttg gtcgggttgc acggccaggg gacgtcctcc tggcccggct ccagcggtgc
     9301 gccgacggag tgcacggcct tgacgaacga gcctctctcc tcgatgcggc gcagtgccct
     9361 gtcccccatc cgcgtcatca cccgcatgga gacggcgacg tacggggagt cggtgatctc
     9421 gacgccgagc tgcgagatct caccgcccag cgggcccatg cagaacggca ccacgtacat
     9481 ggtgcggccg cgcatgcagc ccttgaagag cgtgccgaac gtccggcgca tctcgtccgg
     9541 cgcgacccag ttgttggtgg gccccgcgtc ctcctcccgc tcggagcaga tgaaggtgcg
     9601 gtcctcgacc ctggccacgt cgccggggtc cgacttggca tagaagctgt tgggccgggc
     9661 ggccagccgc gtgaacgtgc cctgctccac gaggaggttc gtcaggcgcg tccactcctc
     9721 ctcggacccg tcgcaccact cgatccggtc cggctgggtc agggcggcga tctccttcac
     9781 ccacgccgcc aattcgcgat tgctggtcgg tgcgggaact tccacggaaa cggacacgac
     9841 ggtgactcct ccactctctc gggcccagtc atcattaacg acgaagcggt gcgaaagcca
     9901 attggcatag acctgtcggc tttggccgtc gtcgcaggca gcgggcccga tgattggggg
     9961 agcctaccca ccgagaacgg atttaaaaga gaaatttcgc gcagtggtcc agtccaatag
    10021 cgctggtcta aaccaatttc cgcagtggtt tagtccattt cccgctccgt ttccgctgga
    10081 cggtctcagg aattgacgtc cggccccgcg gagcgaaccc tgtcgacgtg ctggagcgcg
    10141 tcacgcagat ccgccagcca cgtgtcggtg tgcctgccga ccagtcgcac gcaccaggcc
    10201 agcgcgtcgc tgcggctcct ggccacgccg gcggccacga gcgtgtcgag gacctgccgc
    10261 tccccctggc gcagcctggt catcacgggc acggacagcg tggtgaacat cacagtctcg
    10321 ccaccgcaga ccacgcccca ggagaccttc tgccggaact tgtgctcggc ctcgccggcg
    10381 atctcgatcc gccgctcgcg cgtctcctcc ctgaacctcc tggccagccc ctcgatcagg
    10441 gcggcccgct cggcctccgg cacgtcgccg ggcggatcgg gcagcgtgcc gagcaccgcg
    10501 atctcctcgc ggtcgcggac gatctcgggc gcgccggtga accacccctc gggcagccgc
    10561 ccggcgaacc atccgtgaat gcgtgccgtc tcgtcgtctg tagccatgta atgagaatta
    10621 cacaattgca tcgcaaatgc acgcgtggaa aggcgcccgt tcgctgtggg ggaagcggtc
    10681 agcggacctg ggccgccatg gcggggctca cgcggatgac gcccgcgagg ccgcgggtgt
    10741 gcaccggcga gatcttgacc acgtcaccgg tgcggggcgc ctggatcatc ttcccgtcgc
    10801 cccagtagat cgcgacatgg gagatgtagc tgggattggt gggatcggac cgccagaaga
    10861 gcagatcgcc gggctgggcg tcggccagcc tgacccgcgc cccggtcacc cactgctggt
    10921 gcgtcacccg gggcat
//
