LOCUS       NZ_CP045572            12983 bp    DNA     circular CON 07-NOV-2024
DEFINITION  Nonomuraea phyllanthi strain WYY166 chromosome, complete genome.
ACCESSION   NZ_CP045572
VERSION     NZ_CP045572.1
KEYWORDS    RefSeq.
SOURCE      Nonomuraea phyllanthi
  ORGANISM  Nonomuraea phyllanthi
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 11273186)
  AUTHORS   Ou,Y. and Wu,Y.
  TITLE     Nonomuraea nitratireducens sp. nov., a new actinobacterium isolated
            from Suaeda australis Moq. rhizosphere
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11273186)
  AUTHORS   Ou,Y. and Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2019) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Dongchuan, Shanghai 200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Aug-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: PacBio RSII; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009497075.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 06:36:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,403
            CDSs (total)                      :: 10,325
            Genes (coding)                    :: 10,209
            CDSs (with protein)               :: 10,209
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 22 of 116
            Pseudo Genes (incomplete)         :: 104 of 116
            Pseudo Genes (internal stop)      :: 8 of 116
            Pseudo Genes (multiple problems)  :: 13 of 116
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:05:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7092144
            Orig. end                         :: 7105127
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12983
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="17"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12983
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12983
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7983](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7983)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    414..430
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.63"
                     /tool="antismash"
     gene            complement(437..1105)
                     /locus_tag="GBF35_RS32940"
                     /old_locus_tag="GBF35_32935"
     CDS             complement(437..1105)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544794.1"
                     /locus_tag="GBF35_RS32940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32935"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_153263931.1"
                     /transl_table=11
                     /translation="MATDERLDLPMVGERQSLREQVAQALRAALITGEMRPGVVYSAPV
                     LAAQFGVSATPVREAMLDLAKEGLVEAVRNKGFRVTELSDRDLDELTEIRQLIEVPMVA
                     RLAGEERAEEFERLRPVAEEIVSAAERGDLLAYVDADLRFHVELLSLAGNVHLVQVVRD
                     LRNRARLYGLSQLAERGTLGDSAREHLTLLDALKRGEAETVGQIMREHIGHVRGIWAEH
                     "
     gene            complement(1121..2071)
                     /locus_tag="GBF35_RS32945"
                     /old_locus_tag="GBF35_32940"
     CDS             complement(1121..2071)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544793.1"
                     /locus_tag="GBF35_RS32945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32940"
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_139630524.1"
                     /transl_table=11
                     /translation="MTGLPYLDAATLERLVPMGRATRALEDALLAGLDPEATPQRPVVP
                     VPGGQLLLMPASYGRYAGVKAATVAPGNPERGLPRVQGTYLLFDGETLTPLAAMDGIAL
                     TSLRTPAVSALAVRHLAGEHARRLVVFGSGPQAWGHVPAMRVVRPVTDVTVVGRDAGRA
                     RALAERCRALGLTARALTHDPASREAVGRALGEADIVACCTTAREPLFPGKLAPDGVTV
                     VAVGSHEPGARELDGDLVARATVVVEARAAALEEAGDVLIPIRGGTIAAGHLAGNLAEL
                     VAGEVVIGTGPRVFKSTGMAWEDLVVAAAAYEAWT"
     gene            complement(2068..3072)
                     /locus_tag="GBF35_RS32950"
                     /old_locus_tag="GBF35_32945"
     CDS             complement(2068..3072)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544792.1"
                     /locus_tag="GBF35_RS32950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32945"
                     /product="proline racemase family protein"
                     /protein_id="WP_153263932.1"
                     /transl_table=11
                     /translation="MSQASPAITTVDYHTGGEPFRIVTGGVREIPGADVLSRRATAMAE
                     PEFDGVRRLLCHEPRGHADMYGCFLVPPDDAGALLGALFWHKDGFSTACGHGTIALGAY
                     AVHEGLVAADPDGETDVVVDVPSGRVTARVRCSGGRVRGVTFVSVPSYVMARGVAAGGT
                     KVDISYGGAIYASVPAAALGLSVEPAHLTELIGHARRVKADLAGHPAARHPSDERLSGV
                     YGVIFYDELPDVDLPGLGRAAHQRNVTVFADGEVDRSPCGSGTAARLALLHDAGLRAPL
                     VHDSVVGSTFLARVAEARDGWVVPEIEGMAYRTGRHAFELDPDDPLGTGFTLR"
     gene            complement(3086..4087)
                     /locus_tag="GBF35_RS32955"
                     /old_locus_tag="GBF35_32950"
     CDS             complement(3086..4087)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="GBF35_RS32955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32950"
                     /product="proline racemase family protein"
                     /protein_id="WP_153263933.1"
                     /transl_table=11
                     /translation="MRTKRVFHAVDSHTEGMPTRVITGGIGVIPGASMAERRVYFRDHL
                     DHVRTLLMTEPRGHAAMSGAILQPPTRPDADYGVLFIEVSGLLPMCGHGTIGVATVLVE
                     TGMVEVTEPVTTVRLEVPAGLVEVDVAVEDGQAMSVTLRNVPSYSDRLDAVVRVPGFGE
                     IPYDLAYGGNFYAVVDLDSYGLPFDRKAKNEIIAAGLATMEAINAADEPVHREDDRIRG
                     CHHVYFAAPGSDAHHSRHAMAIFPGWFDRSPCGTGTSARMAQLHARGELPLGRDFVNES
                     FIGTRFVGRLLEETTVGGAPAVLPSITGRAWITGTAQYFLDPRDPFPAGFEL"
     gene            complement(4084..4977)
                     /locus_tag="GBF35_RS32960"
                     /old_locus_tag="GBF35_32955"
     CDS             complement(4084..4977)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892474.1"
                     /locus_tag="GBF35_RS32960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32955"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_153263934.1"
                     /transl_table=11
                     /translation="MENRSKAWHGVMVATALPLRDDLSVDYGAYAEHCRWLVENGCDGV
                     VANGSLGEYQTLTDEERAKVVETAVEAVGGRNVMAGVGAYGSAESRRWAERAGEAGCGS
                     VLLLPPNAYRADERAVVAHYREVAGAGVPVVAYNNPYDTKVDLTPALLARLHEEGLIVA
                     IKEFSGDVRRAYELAELAPELDLLIGSDDVLLELAIAGAVGWIAGYPNALPASTVALYR
                     AAVARDLGTALPLYRTLHPLLRWDSKTEFVQAIKLSMDLAGRHGGPCRQPRQSLTDEQA
                     AIVRSATEKALAEGLR"
     gene            complement(5001..6530)
                     /locus_tag="GBF35_RS32965"
                     /old_locus_tag="GBF35_32960"
     CDS             complement(5001..6530)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="GBF35_RS32965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32960"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_228010363.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.2e-23, bitscore:
                     82.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3e-05, bitscore: 23.6,
                     seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDVTGRTGGPGDGVTYDLVVIGGGPAGVAGALTAAQAGLRVALV
                     DASVRLGGQYFRHSADPRPAPGLGRFLRQARALGRRADVRLRHQVWNVTREPGGGLTVH
                     ATAPDRTAVPLAARRVLIATGAYDRPLPFPGWDLPGVLTAGGAQALLKGDGVLAGRRIV
                     VGGTGPFLLPVASGLAGAGARVLGVFEANGGLGLARHPLLVLGKAGEAAGYAAVLARHR
                     VPYRTRQAVVAAHGEREVEAVTVARLDKEWNVLATRVVECDTVAVGYGFVPQIELGVQL
                     GCRTRQDVDGSPVLAVDAGQRTSVPGVWAAGEPTGVGGWRLSEVEGRIAGLTIAAESRA
                     AESRAAESRAAESRALEGRTDESRPDESRDVEGRDVEGRDREGRDGEVAGVLARRRERW
                     RGFGEALQHAYPVRPGWQGWLRDDTLVCRCEEVPLARVREARDLGATDARSIKLLARPG
                     MGWCQGRICGYAVSCLAGEPPQPPRRPIAQPVTLGALAELTDDDPTTPPLT"
     gene            complement(6527..6769)
                     /locus_tag="GBF35_RS32970"
                     /old_locus_tag="GBF35_32965"
     CDS             complement(6527..6769)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620515.1"
                     /locus_tag="GBF35_RS32970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32965"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_139630520.1"
                     /sec_met_domain="Fer2_4 (E-value: 4.7e-22, bitscore: 77.1,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFHITIDGRPVPVTPGQSIGAALHAAGVRSWRSTRFGGRPRGLF
                     CGIGVCFDCLVSVNGRTPERACLAEAAPGDEVTTA"
     gene            complement(6766..7983)
                     /locus_tag="GBF35_RS32975"
                     /old_locus_tag="GBF35_32970"
     CDS             complement(6766..7983)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547415.1"
                     /locus_tag="GBF35_RS32975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32970"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_153263935.1"
                     /sec_met_domain="DAO (E-value: 1e-61, bitscore: 208.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDVIVVGAGVVGAACAYYAARAGLDVTVVDRGSVAGGTTGSGEG
                     NILVSDKEPGPELDLAVLSNRLWRELAGAHGGTGAHGHAGASGQRDVPGHGGADGGFEF
                     EAKGGLVVAETPEIQRQLAELAAKQDVEHAVVPAAELRDYEPELADDLAGGVFYPQDAQ
                     VQPMLAAARLLRHGAESFGRGTLHLRLGAAVTGFVRDGDRVTGVRTSHGDVLGDAVINA
                     AGTWGGEVAALAGVALPVLPRRGFILVTEPLRRPLIRHKVYTAAYVTNVASDSAGLETS
                     AVVEGTQAGTVLIGASRERVGFDRTTSVPVLARLAAQAVALFPALRDVRAIRSYCGFRP
                     YCPDHLPVIGADPCAPGLYHACGHEGAGIGLAPATGHLLAQVLTGTPPDLDLRPFRPDR
                     FPEESS"
     misc_feature    7996..8012
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.34"
                     /tool="antismash"
     misc_feature    8015..8029
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            8114..9094
                     /locus_tag="GBF35_RS32980"
                     /old_locus_tag="GBF35_32975"
     CDS             8114..9094
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544789.1"
                     /locus_tag="GBF35_RS32980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32975"
                     /product="heme A synthase"
                     /protein_id="WP_153263936.1"
                     /transl_table=11
                     /translation="MKLPTDHPNPLVRSLFSFWAPTAESMRGWALASVVVNAGIAVTGA
                     GVRVTGSGLGCPTWPKCTPDSFIPVAHPELSMLTMLVEFGNRLLTFLVLAVGLACVVAA
                     WRLAPRRRSLMRLALLQPVGIVAQALWGGLVVNTMLNPFTVGMHYLLSVGLIAACWMLY
                     ARAGEGDGPARRVTHRDIRRLGYVLLAAVFALLVVGVAVSGTGPHSGDEMASRFDLDIE
                     AVARLHADVVYIVVGLTFALLFALHISRSERPARRAALVLFAVELGQGVIGYTQYFLAV
                     PAALVLLHVLGSTLVWIAALRVVTSLRTRTPLSTAPSPTEEPAAV"
     gene            complement(9188..10345)
                     /locus_tag="GBF35_RS32985"
                     /old_locus_tag="GBF35_32980"
     CDS             complement(9188..10345)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544787.1"
                     /locus_tag="GBF35_RS32985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32980"
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="WP_139630517.1"
                     /transl_table=11
                     /translation="MATRDPRWVLKDRPSFALIIGLVLTGICALVSFSYDLFDRAPVQF
                     FIGLGLALAPVPLLLAAVLALDRMEPEPRSNLIFAFAWGAGVAVLVAGVINSLNLHYII
                     DTARLSESSARNIAATFGAPVVEETMKGLVLLGLLRFRRAELDGPTDGIIYASMVGLGF
                     AMSENVSYYIAALSTSGVKGLAVTVVLRGILSPFAHPLFSSMIGIAVAYAAQRDGPERV
                     FYILAGWTGAMILHGLWNGLASYGGFPGLVVAYLALLVVLIVLIGVVFRDRRRIVALIQ
                     RYLPPYEPTKLVTEADVFMLSAFPRRRQARQWARLHGGKRGLRAMRDYQLAATELGLLH
                     ERAARRMVDDQTFHEEQRALLECMSTARADFPVVAAQARPFSPPG"
     gene            complement(10409..11353)
                     /locus_tag="GBF35_RS32990"
                     /old_locus_tag="GBF35_32985"
     CDS             complement(10409..11353)
                     /EC_number="2.5.1.141"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ubiA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544786.1"
                     /locus_tag="GBF35_RS32990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBF35_32985"
                     /product="heme o synthase"
                     /protein_id="WP_139630516.1"
                     /sec_met_domain="ubiA (E-value: 2.5e-14, bitscore: 52.5,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVLTNRQATARSPQVEAPRSIGMVVKAYIALTKPRIIELLLITT
                     LPVMFLAAGGLPPLWTSISVMVFGTLSAGSANALNCYVDRDIDQKMRRTRRRPLARHQV
                     SPRGALIFGIVLGALSTVGLWVTTNGLAALLSTGAILFYVLVYSMILKRRTAQNIVWGG
                     IAGCMPVMIGWAGITDRVDWAPLVLFGVVFFWTPPHTWTLAMRYKEDYAAAKVPMLPVV
                     ATERRVILESVAYTWATVLCSLALWPVAGTTLFYPLVAAVLGAMCLWEVHRLLARLNAG
                     KSGVDLRPMRFFHWSNAYLALLFLAVAIDPLLA"
     misc_feature    11594..11609
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.11"
                     /tool="antismash"
     misc_feature    12443..12463
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.1"
                     /tool="antismash"
ORIGIN
        1 cgcggtggcg gcgctcatcg gcgtgctgct gctcggcgag atcctgcacg cgcagcactg
       61 ggcggcgatc atctgcatcg tggccgccag cctcggttcg accaggaggc gcagacctta
      121 gacacgagcc ctgagccggg gtgatgggcc gccgcgcgcc cgtgggatgg gatctcgggc
      181 gcgcggcgga gggctcggct ctggtgggcg gggtgtgccg agcccatgga aggtcagcgg
      241 agtgcgggtg tccgcgtgac caggcatgcg ccatccacct cagtcggtgg tggccggtcc
      301 gctggggacc aggacggcgg ggccgcgagc ggacgcgttc ggcgtcgttc gtccggcccg
      361 caatgagatt accgcgattg gcggccgcgc gatcccggaa tgtcagactt tgatgccaga
      421 aaactgggca tccggctcag tgctcggccc agatgccccg tacgtggccg atgtgctctc
      481 tcatgatctg cccgacggtc tcggcctcgc ccctcttcag cgcgtcgagc agcgtgaggt
      541 gctccctggc cgagtcgccg agcgtgccgc gctccgcgag ctgggacagg ccgtagagcc
      601 tggccctgtt gcgcaggtcg cgcaccacct ggaccaggtg gacgttgccc gccagggaca
      661 gcagctcgac gtggaagcgc aggtcggcgt ccacgtacgc gagcaggtcg ccgcgctccg
      721 cggccgagac gatctcctcg gcgacgggcc tgagccgctc gaactcctcg gcccgctcct
      781 cgccggccag cctggccacc atgggcacct cgatgagctg gcggatctcg gtgagctcgt
      841 cgaggtcgcg gtcggacagc tcggtcaccc tgaagccctt gttgcgcacc gcctcgacca
      901 ggccctcctt ggcgaggtcg agcatggcct cgcggaccgg cgtggcggag acgccgaact
      961 gggcggccag caccggcgcg gagtagacca cgcccggccg catctcgccg gtgatcagcg
     1021 ccgcgcggag cgcctgcgcg acctgctcgc gcaggctctg ccgctctccc accatcggca
     1081 ggtcgagccg ctcgtcggtg gccatcgggc ccatcccttc ctaagtccac gcctcgtaag
     1141 cggcggccgc gaccacgagg tcctcccacg ccatgcccgt gctcttgaac acgcgcggcc
     1201 ccgtgccgat gacgacctcg cccgccacga gctcggcgag gttaccggcg agatggccgg
     1261 cggcgatcgt accgccgcgg atcgggatga gcacatcccc cgcctcctcc agggcggcgg
     1321 ccctggcctc gacgaccacg gtggcgcggg cgacgaggtc gccgtcgagc tcgcgggcgc
     1381 cgggctcgtg cgacccgacc gcgacgacgg tgacgccgtc gggggcgagc ttaccgggga
     1441 acagcggctc cctcgccgtc gtgcagcagg cgacgatgtc cgcctccccc agcgcccgac
     1501 cgaccgcctc ccgggaggcg gggtcgtggg tcagggcgcg ggcggtcagg ccgagcgcgc
     1561 ggcagcgctc ggcgagcgcc ctcgcccggc ccgcgtcgcg cccgaccacc gtgacgtccg
     1621 tgaccggccg caccacccgc atcgccggca cgtgccccca cgcctgcggc ccgctgccga
     1681 acaccaccag ccgccgggcg tgctcgccgg ccaggtggcg cacggcgagg gccgagacgg
     1741 cgggggtgcg cagcgaggtg agcgcgatcc cgtccatggc ggcgagcggt gtcagggtct
     1801 cgccgtcgaa cagcaggtac gtgccctgga cccggggcag cccgcgctcg gggttgccgg
     1861 gcgcgacggt ggcggccttg acgccggcgt accgcccgta ggaggccggc atgagcagga
     1921 gctggccgcc cggcacgggc acgaccggcc gctgcggcgt ggcctcgggg tcgagcccgg
     1981 ccagcagcgc gtcctccagc gcccgggtgg cgcgccccat ggggaccagc cgctccagcg
     2041 tggcggcgtc gaggtacggc aggccggtca tcgcagcgtg aaccccgttc ccaggggatc
     2101 gtccggatcg agctcgaagg cgtgccgccc ggtccggtac gccatgccct cgatctccgg
     2161 caccacccac ccgtcacgcg cctcggccac ccgcgccagg aacgtgctgc cgaccacgct
     2221 gtcgtggacc agcggtgcgc gcagcccggc gtcgtgcagc agcgccagcc gggccgcggt
     2281 gcccgacccg cagggcgagc ggtccacctc cccgtcggcg aacaccgtca cgttccgctg
     2341 gtgcgccgcc ctgcccaggc cgggcaggtc cacgtcgggc agctcgtcgt agaagatcac
     2401 gccgtagacg ccggacagcc gctcgtcgga cgggtgccgg gccgccgggt ggcccgccag
     2461 gtccgccttg acgcggcggg cgtggccgat gagctccgtc aggtgcgcgg gctccacgga
     2521 caggcccagc gccgccgccg ggaccgacgc gtagatcgcg ccgccgtacg agatgtcgac
     2581 cttcgtgccg ccggccgcca cgccgcgcgc catcacgtac gacggcacgc tgacgaacgt
     2641 caccccgcgc acccgcccgc ccgagcagcg cacccgggcc gtgacgcgcc ccgacggcac
     2701 gtcgacgacc acgtccgtct cgccgtcggg atcggccgcg accaggccct cgtggacggc
     2761 gtacgcgccc agcgcgatcg tgccgtgccc gcaggccgtg ctgaagccgt ccttgtgcca
     2821 gaacagcgcc ccgagcaggg ctccggcgtc gtcgggcggg acgaggaaac agccgtacat
     2881 gtcggcgtgc ccgcgcggct cgtggcacag cagccgccgc acgccgtcga actcgggctc
     2941 ggccatggcg gtggcccgcc gcgagagcac gtcggcgccg gggatctccc gcacgccgcc
     3001 ggtcacgatc cggaacggct cgccaccggt gtggtagtcg accgtcgtga tcgcgggact
     3061 cgcctggctc acgcgccccg ccccctcaca gctcgaaccc ggccgggaac gggtcgcgag
     3121 ggtcgaggaa gtactgggcg gtgccggtga tccaggcgcg gccggtgatg gacgggagca
     3181 cggccggggc gccgcccacg gtcgtctcct ccagcagccg gccgacgaac ctggtgccga
     3241 tgaacgactc gttcacgaag tcccggccga gcggcagctc gccgcgggcg tgcagctgcg
     3301 ccatgcgggc gctggtgccg gtgccgcacg gcgagcggtc gaaccagccg gggaagatgg
     3361 ccatggcgtg ccgcgagtgg tgggcgtcgg agccgggcgc ggcgaagtag acgtggtggc
     3421 agccgcggat gcggtcgtcc tcgcggtgca cgggctcgtc ggcggcgttg atcgcctcca
     3481 tggtggccag gccggcggcg atgatctcgt tcttggcctt gcggtcgaag ggcaggccgt
     3541 aggagtcgag gtcgacgacg gcgtagaagt tgccgccgta cgccaggtcg tacgggatct
     3601 ccccgaaccc gggcacgcgc acgacggcgt ccagccggtc gctgtaggac ggaacgttcc
     3661 ggagcgtgac cgacatcgcc tgcccgtcct ccacggccac gtccacctcg acgagcccgg
     3721 ccgggacctc caggcgcacg gtggtgaccg gctcggtcac ctccaccatg ccggtctcga
     3781 cgagcacggt ggccacgccg atggtgccgt gcccgcacat cggcagcagc ccggacacct
     3841 cgatgaacag cacgccgtag tcggcgtccg gcctggtggg cggctggagg atggcgccgc
     3901 tcatggccgc gtggccgcgc ggctcggtca tcagcagggt gcggacgtgg tcgaggtggt
     3961 cgcggaagta gacccgccgc tccgccatgc tcgcgccggg gatcacgccg atcccgccgg
     4021 tgatgacgcg ggtgggcatc ccctccgtgt gggagtcgac ggcgtggaag acccgcttcg
     4081 tcctcatcgc agcccttctg ccagtgcctt ctcggtcgcc gatcgcacga tagcggcctg
     4141 ctcgtccgtc agcgactgcc tcggctgcct gcacggcccg ccgtggcggc ccgccaggtc
     4201 catggacagc ttgatggcct ggacgaactc cgtcttggag tcccagcgca gcagcggatg
     4261 cagggtccgg tagaggggca gtgcggtgcc gagatcgcgg gcgaccgccg cgcggtagag
     4321 ggcgacggtg gaggcgggca gcgcgttggg gtagccggcg atccagccga ccgccccggc
     4381 gatggcgagc tccagcagca cgtcgtcgga gccgatcagc aggtccagct cgggggccag
     4441 ctcggccagc tcgtacgccc gccgcacgtc gccgctgaac tccttgatcg ccacgatcag
     4501 cccctcctcg tgcagcctgg ccagcagcgc gggggtgagg tcgaccttgg tgtcgtaggg
     4561 gttgttgtag gccacgaccg gcacgcccgc cccggcgacc tcgcggtagt gggcgacgac
     4621 ggcccgctcg tcggcgcggt aggcgttcgg gggcagcagg agcaccgagc cgcagccggc
     4681 ctcgcccgcc cgctcggccc agcggcggga ctcggcggag ccgtacgcgc cgaccccggc
     4741 catcacgttg cgcccgccga cggcctccac cgcggtctcc accaccttgg cgcgctcctc
     4801 gtcggtgagc gtctggtact cgcccagcga cccgttcgcg accacgccgt cgcagccgtt
     4861 ctccaccagc cagcggcagt gctcggcgta ggcgccgtag tcgacggaca ggtcgtcgcg
     4921 caggggcagc gccgtggcga ccatgacgcc gtgccaggcc ttgctgcggt tctccatcgg
     4981 gaagtcctcc atgtctgggg tcacgtgagc ggtggggtcg tggggtcgtc gtcggtgagc
     5041 tcggccagag cgccgagggt gacgggctgg gcgatggggc ggcggggcgg ctgcggcggc
     5101 tccccggcca ggcaggagac ggcgtagccg cagatgcggc cctggcacca gcccatgccg
     5161 ggcctggcca gcagcttgat cgagcgcgcg tcggtggcgc cgaggtcgcg cgcctcccgg
     5221 acgcgggcca gcggcacctc ctcgcaccgg cacaccagcg tgtcgtcgcg cagccagccc
     5281 tgccagccgg gccggacggg gtaggcgtgc tgcaacgcct ccccgaaccc gcgccaccgc
     5341 tcgcgccgcc gcgccagcac cccggccacc tcgccatcgc gcccctcacg gtcgcgccct
     5401 tcgacgtcgc gcccttcgac gtcgcggctt tcgtcggggc ggctttcgtc ggtgcggcct
     5461 tcaagggcgc ggctttcggc ggcgcggctt tcggcggcgc ggctttcggc ggcgcggctt
     5521 tcggcggcga tggtgaggcc cgcgatgcgc ccctcgacct ccgacaaccg ccagccgccc
     5581 acgccggtgg gctcgcccgc ggcccagacc cccgggacgc tggtgcgctg gccggcgtcg
     5641 accgcgagca cggggctgcc gtccacgtcc tgccgcgtcc ggcagccgag ctgcacgccg
     5701 agctcgatct gcggcacgaa cccgtacccg acggccaccg tgtcgcattc gacgacacgg
     5761 gtggccagaa cgttccattc cttgtcgagg cgggccacgg tgacggcctc cacctcgcgc
     5821 tccccgtgcg ccgccacgac cgcctggcgg gtccggtacg ggacccggtg gcgggccagc
     5881 acggcggcgt acccggcggc ctccccggcc ttgccgagga ccagcagcgg gtgcctggcc
     5941 aggccgagcc cgccgttcgc ctcgaacacc ccgagcaccc gcgcgcccgc cccggccagc
     6001 ccggaggcca cgggcagcag gaacggcccg gtgccgccga ccacgatccg ccggcccgcc
     6061 aggacgccgt cgcccttgag cagcgcctgc gcgccgccgg cggtgagcac gccgggcagg
     6121 tcccagccgg ggaacggcag cggccggtcg tacgcgccgg tcgcgatcag cacgcgccgc
     6181 gccgccagcg ggaccgcggt cctgtcgggg gccgtggcgt gcacggtcag gccgccgccc
     6241 ggctcgcgcg tgacgttcca cacctggtgc cgcagccgga cgtcggcgcg gcggccgagg
     6301 gcgcgggcct ggcgcaggaa gcggccgagc ccgggcgcgg ggcgcgggtc ggcggagtgg
     6361 cggaagtact ggccgccgag ccgtacggag gcgtccacga gcgccacccg cagcccggcc
     6421 tgggccgcgg tcagcgcgcc cgccaccccg gcgggcccgc ccccgatcac caccaggtcg
     6481 tacgtcacgc cgtctccagg gccgcccgtc ctgcctgtca cgtctgtcac gccgtcgtca
     6541 cctcgtcgcc gggcgcggcc tcggcgaggc aggcgcgttc gggcgtgcgc ccgttgacgg
     6601 agaccaggca gtcgaagcag acgccgatgc cgcagaacag cccgcgcggc cgaccgccga
     6661 agcgggtgct gcgccacgag cgcaccccgg cggcgtgcag ggccgccccg atgctctgcc
     6721 cgggcgtcac cgggacgggc cgcccgtcga tcgtgatgtg gaacgtcaag aggactcctc
     6781 cgggaagcgg tccggccgga agggccgcag gtccaggtcc ggcggggtgc cggtgagcac
     6841 ctgcgcgagc aggtggccgg tggccggggc gagcccgatg cccgcgccct cgtgcccgca
     6901 cgcgtggtac agcccggggg cgcacggatc ggcgccgatg acgggcaggt ggtcggggca
     6961 gtacggccgg aacccgcagt acgacctgat ggcccgcacg tcgcgcagcg cggggaacag
     7021 cgccaccgcc tgcgcggcca gccgggccag caccggcacc gacgtggtgc ggtcgaagcc
     7081 gacccgctcc ctgctcgccc cgatgagcac ggtccccgcc tgggtgccct ccacgacggc
     7141 ggaggtctcc agcccggcgg agtcgctggc cacgttggtg acgtaggcgg cggtgtagac
     7201 cttgtgcctg atcagcggcc tcctcagcgg ctccgtgacc aggatgaacc cccgcctggg
     7261 caggaccggc agcgccaccc ccgccagcgc ggccacctcg ccgccccagg tgccggccgc
     7321 gttgatgacc gcgtcgccga gcacgtcgcc gtgggaggtg cgcacgcccg tcacccggtc
     7381 gccgtcgcgg acgaacccgg tcaccgccgc cccgaggcgg aggtggagcg tgccccggcc
     7441 gaagctctcc gcgccgtgcc gcagcagccg cgcggcggcc agcatgggct gcacctgcgc
     7501 gtcctgcggg tagaacaccc cgccggccag gtcgtccgcc agctccggct cgtagtcgcg
     7561 cagctcggcg gcgggcacca cggcgtgctc gacgtcctgc ttggcggcca gctcggcgag
     7621 ctgccgctgg atttccggcg tctcggcgac gaccaggccg cccttcgcct cgaactcgaa
     7681 cccgccgtcc gcgccgccgt gccccggcac gtcccgctgc ccggaagcac cggcgtgccc
     7741 gtgggcgccg gtccccccgt gggcgccggc cagctcgcgc cacagccggt tcgacagcac
     7801 ggcgaggtcc agctcagggc ccggctcctt gtcggacacc aggatgttgc cctcgcccga
     7861 gcccgtcgtg ccgcccgcga cggagccgcg gtcgaccacg gtcacgtcca ggccggccct
     7921 cgcggcgtag taggcgcagg cggcgcccac gactccggcg ccgaccacga tgacgtcgga
     7981 catcctcacc tcccatcagt aatatgtcac atactcttcc ctttgagaag ccccccgcag
     8041 gcgctcctcc cgggcggaga aggcggggtg accggtggcg ggaccggcca catcggcccg
     8101 taccatcgat gacgtgaagc tacccacgga ccaccccaac cctctcgtgc gcagcctgtt
     8161 ctccttctgg gctcccacag cggagtccat gcggggctgg gcactggcgt ccgtggtggt
     8221 caacgccggc atcgccgtga ccggcgcggg ggtgcgggtc accgggtcag ggctcggctg
     8281 cccgacctgg ccgaagtgca cgcccgacag cttcatcccg gtcgcgcacc ccgagctctc
     8341 gatgctcacc atgctggtcg agttcggcaa caggctgctg acgttcctgg tgctggcggt
     8401 ggggctggcg tgcgtggtgg cggcgtggcg gctggcgccg cgcaggcgat cgctcatgcg
     8461 gctggcgctg ctgcagccgg tcggcatcgt ggcccaggcg ctgtggggcg ggctggtggt
     8521 gaacaccatg ctcaacccgt tcacggtggg catgcactac ctcctctccg tcggcctgat
     8581 cgcggcctgc tggatgctgt acgcccgcgc gggcgagggc gacggcccgg ccaggcgggt
     8641 gacccaccgt gacatccgca ggctcggcta cgtgctgctg gccgccgtgt tcgcgctgct
     8701 ggtggtcggc gtcgcggtga gcggcacggg cccgcactcg ggtgacgaga tggcctcccg
     8761 cttcgacctg gacatcgagg ccgtggccag gctgcacgcc gacgtggtct acatcgtggt
     8821 ggggctgacg ttcgcactgc tgttcgcgct gcacatcagc cgctcggagc ggcccgccag
     8881 gcgggcggcg ctggtgctgt tcgcggtgga gctgggccag ggcgtgatcg gctacaccca
     8941 gtacttcctg gccgtgccgg cggcgctggt gctcctgcac gtgctcggct cgacgctggt
     9001 ctggatcgcc gcgctcaggg tggtcacctc gctgcgcacc cgcacgccgc tgagcaccgc
     9061 cccctccccc accgaagagc ccgccgccgt ctgagcggtt cagccccgat atggggtgat
     9121 aggtccgcca ctcccccacc cgcccgaggg tgcgatttgc cgagaaatgt ccgccccgct
     9181 cggaggctca gccaggcggc gagaagggcc gggcctgcgc cgccaccacc gggaagtcgg
     9241 cccgcgcggt gctcatgcac tccagcagcg cccgctgctc ctcgtggaag gtctggtcgt
     9301 cgaccatgcg gcgggcggcg cgctcgtgca gcaggcccag ctcggtggcg gccagctggt
     9361 agtcgcgcat ggcgcgcagc ccgcgcttgc cgccgtggag cctcgcccac tgccgggcct
     9421 gccgcctcct ggggaacgcc gagagcatga agacgtcggc ctcggtgacc agcttggtcg
     9481 gctcgtacgg cggcagatag cgctggatga gggccacgat gcgccgccgg tctctgaaca
     9541 ccaccccgat gagcacgatc agcacgacca gcagcgccag gtaggccacc acgagcccgg
     9601 ggaagccgcc gtaggaggcc aggccgttcc acaggccgtg caggatcatc gcgcccgtcc
     9661 agcctgccag gatgtagaag acccgctcgg ggccgtcacg ctgggccgcg taggcgaccg
     9721 cgatgccgat catggagctg aacagcgggt gggcgaacgg cgacaggatg ccgcgcagca
     9781 ccacggtgac cgccagcccc ttgaccccgg acgtggacag cgcggcgatg tagtagctga
     9841 cgttctcgct catggcgaag cccagcccga ccatgctggc gtagatgatg ccgtcggtgg
     9901 ggccgtcgag ctcggccctg cggaacctca gcagcccgag cagcaccagc cccttcatcg
     9961 tctcctccac caccggagcg ccgaacgtgg ccgcgatgtt gcgggcgctg gactccgaca
    10021 gccgggcggt gtcgatgatg tagtgcaggt tgagcgagtt gatcacgccg gcgaccagca
    10081 ccgcgacgcc cgcgccccac gcgaaggcga agatgaggtt gctgcgcggc tcgggctcca
    10141 tgcggtcgag cgcgagcacc gccgcgagca gcagcggcac cggcgcgagc gccagcccga
    10201 gcccgatgaa gaactgcacc ggagcgcggt cgaacaggtc gtaggagaac gagacgagag
    10261 cgcagatgcc ggtgaggacc agcccgatga tcaacgcgaa cgacgggcgg tccttcaaca
    10321 cccagcgtgg atcacgggtc gccatacggt gactttaacg gatcagagat tgggcccggc
    10381 ctcaccgtac ggtcacgcct ccgggagctc aggccagcag cgggtcgatc gccacggcga
    10441 ggaacagcag cgccaggtag gcgttggacc agtggaagaa gcgcatgggc cgcagatcga
    10501 cgccgctctt gccggcgttg agccgggcca gcaggcggtg cacctcccac aggcacatgg
    10561 cgccgagcac ggccgccacg agggggtaga acagggtggt gccggccacc ggccacagcg
    10621 ccagcgagca cagcacggtc gcccacgtgt aggcgacgga ctcgaggatc acccgccgct
    10681 cggtggccac cacgggcagc atgggcacct tggccgccgc gtagtcctcc ttgtagcgca
    10741 tggccagcgt ccaggtgtgc ggcggcgtcc agaagaacac cacgccgaac agcacgagcg
    10801 gcgcccagtc gacccggtcg gtgatgccgg cccagccgat catcaccggc atgcagcccg
    10861 cgatgccgcc ccacacgatg ttctgggccg tgcggcgctt cagaatcatc gagtagacca
    10921 gcacgtagaa caggatcgcc ccggtggaca gcagcgcggc cagcccgttg gtggtcaccc
    10981 agaggcccac ggtcgacagc gcgccgagca cgatgccgaa gatcagcgcg ccgcgcggcg
    11041 agacctgatg cctggccagc ggcctgcggc gggtgcggcg catcttctgg tcgatgtcgc
    11101 ggtcgacgta gcagttgagc gcgttggcgc tgcccgccga tagggtgccg aacaccatga
    11161 ccgagatcga cgtccacagc ggcggcagac cgccggccgc caggaacatc accggcagtg
    11221 tcgtgatcag cagcaactcg atgatccgcg gcttggtgag cgcgatgtag gccttcacga
    11281 ccatgccgat cgagcgcggc gcctccacct gcggagatcg ggccgtcgcc tgcctgttgg
    11341 tcagcaccgt caaggcgctt ccagcctgtg cggagtcaac gcgtaacctc tgaatagagc
    11401 ggatccatgg gtgggcgccg tagcggccct gcggacaaac aaactgtagt cgcagggacg
    11461 gccggtccgg ccaatggggg gcttgagccg acatggacca gtgcgagtgg ggacgcgcgg
    11521 ggggaagagg aattgatcgc cgactcgact cggttagggt cccttgtggg cgggattgcc
    11581 aaccggcaca accaatgaca actagattcc ggagatcgag cacttgagca ccgaactcgt
    11641 gccagccctc gaatggaccg acctggacaa gcaggcggtc gatgtcgttc gagccctggc
    11701 gatggacgca gtggagaaag cgggctcggg tcatcccggc accgccatga gcctggcccc
    11761 cgccgcatac ttgctgttcc agcgtgtgat gcgccacgac ccgaccgatc ccaactggat
    11821 cgggcgcgat cggttcgtgc tgtcctgcgg ccactccagc ctgactctct acatccagct
    11881 ctacctttcc ggctacggcc tcacgctcga cgacctgcgg cggctgcgcc agtgggacag
    11941 cctgacgccg ggccaccccg agcacggcca cacggcgggc gtggagacca cgacgggccc
    12001 gctcggccag ggcctgggca acgcggtcgg catggcgatg gccgcccgcc gcgagcgcgg
    12061 cctgttcgat cccgacaccg acaacgggtc ctcgccgttc gaccacacca tctggtgcat
    12121 cgtcagcgac ggcgacatcg aggagggcat cagccacgag gccagcgcga tcgccggcca
    12181 ccagaagctg ggcaacctcg tcgtcctcta tgacgacaac cacatctcca tcgaggacga
    12241 caccctcatc gccaagtcgg aggacgtcgt cgcccgctac gaggcgtacg gctggcacac
    12301 ccagagcgtc gactggaccg ccaccggcga gtacgccgag gacgtccagg cgctctacga
    12361 cgcgctgcag gcggccaagg ccgagaccga ccggccgtcg ttcatccggc tgcgcacgat
    12421 catcggctgg ccggccccga acaagcagaa caccggcaag atccacggct cggccctggg
    12481 tgaggcggag gtcgccgcca ccaaggaagt cctgggcatg gacccgggca agaccttcga
    12541 cgtgccggcc acggtgctgg agcacgcccg cggcatcgcc cagcgcggcc gcaccgagca
    12601 cgccgagtgg gacaagcgct actccgaatg gcggctcgcc aacgaggagc gggcggccga
    12661 gttcgaccgc atctcccacc atgagctgcc caacggctgg gccaaggcgc tgccgtcgtt
    12721 cgagccgggc gcctcgatgg cgacccgcaa ggcgtccggc gaggtgctga gcgcgctggc
    12781 gccggtgctg cccgagctgt ggggcggctc ggccgacctg gcggagtcga acaacaccac
    12841 gatgaagggc gaggtgtcct tcatccccga ggagtaccag accagggagt tcccgggtaa
    12901 ccgctacggc cgcacgctgc acttcggcat ccgcgagcac gccatgggcg cgatactcaa
    12961 cggcatcgcc ctgcacggcg gca
//
