LOCUS       NZ_CP045609            13286 bp    DNA     circular CON 16-FEB-2024
DEFINITION  Bacillus cereus strain SB1 plasmid p3, complete sequence.
ACCESSION   NZ_CP045609
VERSION     NZ_CP045609.2
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Bacillus cereus SB1 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2019) Department of Physiology, Anatomy &
            Microbiology, La Trobe University, Kingsbury Drive, Bundoora,
            Victoria 3086, Australia
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 13286)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2019) Department of Physiology, Anatomy &
            Microbiology, La Trobe University, Kingsbury Drive, Bundoora,
            Victoria 3086, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 46x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/16/2024 03:34:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,375
            CDSs (total)                      :: 6,221
            Genes (coding)                    :: 5,991
            CDSs (with protein)               :: 5,991
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 140 of 230
            Pseudo Genes (internal stop)      :: 70 of 230
            Pseudo Genes (multiple problems)  :: 57 of 230
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:27:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13286
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045609.2.
            On Dec 19, 2019 this sequence version replaced NZ_CP045609.1.
            Bacteria and source DNA available from Steve Petrovski, La Trobe
            University, Bundoora, 3086, VIC, Australia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13286
                     /collection_date="2018-10-25"
                     /country="Australia:Melbourne"
                     /db_xref="taxon:1396"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus cereus"
                     /plasmid="p3"
                     /strain="SB1"
     region          1..13286
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="glycocin"
                     /region_number="1"
                     /rules="(glycocin or sublancin)"
                     /tool="antismash"
     cand_cluster    1..13286
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(glycocin or sublancin)"
                     /kind="single"
                     /product="glycocin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13286
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8368:8557](-)"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(8369..8557)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
     misc_feature    complement(891..906)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.71"
                     /tool="antismash"
     gene            999..1562
                     /locus_tag="GE376_RS31195"
                     /old_locus_tag="GE376_32105"
     CDS             999..1562
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730001.1"
                     /locus_tag="GE376_RS31195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32105"
                     /product="hypothetical protein"
                     /protein_id="WP_153218715.1"
                     /transl_table=11
                     /translation="MAAPRKHIRILKTKEVEGMDMLWKTGTATREQMEREYNIKGDRLK
                     KLCHSGYLEERTGKIVLGEKGIEKFKKEGKEYQYKTGINNAKHDIRLSEKYISLPKETR
                     ETWKTEKQLHSEAQKDPRYDDFKKRIVESHPQGKFQPTPDGAVYSEVHGGYIAIEVTTR
                     NYKEIDIQQKQEFAKTFLSGYEQL"
     gene            1574..2725
                     /locus_tag="GE376_RS31200"
                     /old_locus_tag="GE376_32110"
     CDS             1574..2725
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016084285.1"
                     /locus_tag="GE376_RS31200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32110"
                     /product="hypothetical protein"
                     /protein_id="WP_153218716.1"
                     /transl_table=11
                     /translation="MATQPTFIVPPTFYTVHEIMNNTNSPYILNLHDIQENPHKRKLSD
                     ELHLFIIKHYHNLFTFMLHHQPYMLKSQFLDLFGYQKGKKNKSRTLGYAKLKELEDKLL
                     IGSKSFYGAEYIYLSYRAYYYFGQEKKVLPIAPGENVLFRHFTRMEDYLELNKQYTDPT
                     LLIALLCSHEHYYLLHTEPFFPIQPIQEKNTRYFGEYFPNNKIPKNLDMEFITNFLIKT
                     KQENRGLWLINEETPETQLHVLITFIDELFKILTTRCSLKLLINDQKESLRIIANIIPI
                     PGTSYITINNLISDIYHIEFLLKICFPTVITSFTFIVMNQDHERELNNVISRITKLREN
                     KKNLQPWQSMIDPKNIKIRNTNLFRRLNFNDSYESQREKPLPQ"
     gene            complement(3254..3838)
                     /locus_tag="GE376_RS31205"
                     /old_locus_tag="GE376_32115"
     CDS             complement(3254..3838)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033344.1"
                     /locus_tag="GE376_RS31205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32115"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000185579.1"
                     /transl_table=11
                     /translation="MTNTINLKQAEKNARLREIEDSKILSEEEMYLANELQAKASSRGM
                     KLVPERKVKNKAKFVQIIQQNIQYLFEIDYLTNAEKVFLLDISMCVGFLSNCLVSDINS
                     KEQIPLTQRELAKKIGRHETKVSPLVRKLIDKGIIARSESGVDDNNVRAYALFINPHVM
                     FSGNRDEVNPTLKAMFRKVPKQLKNLPIKLF"
     gene            complement(4065..4478)
                     /locus_tag="GE376_RS31210"
                     /old_locus_tag="GE376_32120"
     CDS             complement(4065..4478)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /locus_tag="GE376_RS31210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32120"
                     /product="disulfide bond formation protein B"
                     /protein_id="WP_153218717.1"
                     /transl_table=11
                     /translation="MKIKIITRIQFLYVSFCISFLSIIGSLYFSEILGLKPCDLCWYQR
                     IFMYPIPLIVLVSILTNDDKVKNYIRGFSLVGLLIAMYQYIIQLSHTKSSFCSIKSDCS
                     TIQVEWFGFITLPLLSFFAFSIIFMFSFLINKE"
     gene            complement(4497..5768)
                     /locus_tag="GE376_RS31215"
                     /old_locus_tag="GE376_32125"
     CDS             complement(4497..5768)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002130752.1"
                     /locus_tag="GE376_RS31215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32125"
                     /product="SunS family peptide S-glycosyltransferase"
                     /protein_id="WP_153218718.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.3e-22,
                     bitscore: 69.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLYEFYQLLKEKNPNSQLFQDMCLIHDIHRYYYIDLNFENEFNN
                     IIDLYEKSDFPSISCGIITYNEERCIHRCLDSIINEFSEIVVLDSMSNDKTVDIIRENF
                     PQVKVISESWKNDFSYQRNKLINYVTNDWIYFIDADNYFGKNNTSKFKRIARLIEFFNI
                     KCVISPIIHEYTGDIYTDTRRMFSLNQNISFNGKVHEEPIFDGGEIPDNITVKVDVYHD
                     GYDPDIVDMVAKNERNLVLTKEMIQLEPGNPKWLFFYARELNQAKSDKRLVESVLLSAI
                     DLYKFKSDKRFLMESLSLLCKIYFELKDFKSLIKYISVIEEEYPDFYDIEFYKASLLLL
                     DIENRINTVIESLHVALDKTNENTCSTIHSKGDHFKHLLINLYSFSRRWDIVFELYNDI
                     QTLELKQDFLNNVTELKSKIDEIL"
     gene            complement(5773..6192)
                     /locus_tag="GE376_RS31220"
                     /old_locus_tag="GE376_32130"
     CDS             complement(5773..6192)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390029.1"
                     /locus_tag="GE376_RS31220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32130"
                     /product="thioredoxin family protein"
                     /protein_id="WP_153218719.1"
                     /transl_table=11
                     /translation="MKKWFFVFLILLLSIGSAFYIFNSKTLEKNFYTNIDLKEYEQSVN
                     SNDDVYIYVYKTSCPSCQQLKPHLNEFIKNNKLKLKALNIEEEKNYNETFLDKYKLNKT
                     PTILHYKDGREAERLEGYRPEKLKEFFQKSINKEG"
     gene            complement(6189..8291)
                     /locus_tag="GE376_RS31225"
                     /old_locus_tag="GE376_32135"
     CDS             complement(6189..8291)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002130748.1"
                     /locus_tag="GE376_RS31225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32135"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_153218720.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.1e-29, bitscore:
                     92.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKNKFIHTIQHNEHDCGLACVSSLLRYYGFNYGIDYLRDLMVDK
                     KGYSLKDLTFLLGNFKGITYKSLRINEEKLKEALNYIKKPSIALIEREGEKHYIVIYSI
                     KGNKLVISDPLKNKISTMLIKDFSNVFSGVLLVVESVDQNKNDIFNQRYNKANLFKGVL
                     KENKFLFTLTFILSVLVVGIAIILSFFVKFLTDLVIPMRLDTTLIQFSLVFLVINFVKV
                     LFDYIRNYFVVRISYNLDKGLAEKYFDKIVNLPIKFFENRDDGEIISRFRDSHYIRNAF
                     SVNVVTGILDIFIILGISVFLYRMNDVLFFTVLVPVLLLTLCAIMFYEILNKRSRELMV
                     NEAKTTSFLVPFIKNMPTIYSFNKEKYFLDSFDVFFKKQINSALKEFKTVNINNAVRLL
                     IHSSFSIIILWVGTQQVLNDSITLGTLLLINSLAMFLLSSLERLISLQPELQHAVIATE
                     RFLDIVDYPVFNAKDTKKIPNIHDIEFKGFNFGYDNSNRVIENVNMKIFKNDRILLLGE
                     SGVGKSTLVKNLVKFYDVSPFQILVNGIDINKINTSTLREKIIYLNENPFLFKGTIREN
                     LCMGKKISSDEIIAACEVAEIWDVVSVLNDNLDFKLNENANNLSTGQKQRLSLARAILH
                     NPEVLILDESLSNVDLEKFERIHNNLLKMNFTLIVISHTSQMLKNYNRKFVFKDKTLIE
                     EKEVVS"
     gene            complement(8369..8557)
                     /locus_tag="GE376_RS31230"
                     /old_locus_tag="GE376_32140"
     CDS             complement(8369..8557)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: sublancin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002130747.1"
                     /locus_tag="GE376_RS31230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32140"
                     /product="sublancin family glycopeptide"
                     /protein_id="WP_098593232.1"
                     /sec_met_domain="sublancin (E-value: 3.3e-21, bitscore:
                     65.3, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDLFKELKVEELDKHTGHGGMGWAQCAALLAQCSSGGRIGCGGT
                     ATQAYGQCNTYRKMCNK"
     misc_feature    complement(8601..8612)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.09"
                     /tool="antismash"
     misc_feature    complement(8740..8753)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
     gene            complement(8797..9186)
                     /locus_tag="GE376_RS31235"
                     /old_locus_tag="GE376_32145"
     CDS             complement(8797..9186)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016089027.1"
                     /locus_tag="GE376_RS31235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32145"
                     /product="PH domain-containing protein"
                     /protein_id="WP_153218721.1"
                     /transl_table=11
                     /translation="MLKQILLEENEKVRNQIKAYYKYKDLTSLSMLGGNISSSPDVFGL
                     LLTTNRRIIFYTETMKMNKILFEIDYGEIIEFKEQKQTVGLFKKIPSIVICHKKGEEVF
                     STMGDLEKFSELKSFFDGIILGEKA"
     gene            9589..10161
                     /locus_tag="GE376_RS31240"
                     /old_locus_tag="GE376_32150"
     CDS             9589..10161
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674723.1"
                     /locus_tag="GE376_RS31240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32150"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_153218722.1"
                     /transl_table=11
                     /translation="MNKKPHLIDVQPIRNKEQIEDMKWALKRHCSERDYILFLIGIHTG
                     LRVSDLLQLETQTIVKLKQKKRKEFKIKEGKTKKERVINLTSIFDEVYSYAQTLENTWL
                     FPSRKGNQPISKIQAYRQLQKARDFSGVESIGTHTMRKTFGYWFYKQTKDVAMLQDILN
                     HSTPQITLKYIGINKEEKDNILDTFQI"
     gene            complement(10197..10514)
                     /locus_tag="GE376_RS31245"
                     /old_locus_tag="GE376_32155"
     CDS             complement(10197..10514)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499737.1"
                     /locus_tag="GE376_RS31245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32155"
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="WP_153218723.1"
                     /transl_table=11
                     /translation="MYHYNSYEYNDDVRQSINRFYEHINGQGMSFTLNSGERLSYVGDP
                     WNDRISSVSVAPRTLVILYTDINYGHPVKILENFGNTSYLFNIHQDFNDVTSSIKTFRI
                     W"
     misc_feature    10716..10729
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.18"
                     /tool="antismash"
     gene            complement(10794..11402)
                     /locus_tag="GE376_RS31250"
                     /old_locus_tag="GE376_32160"
     CDS             complement(10794..11402)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855467.1"
                     /locus_tag="GE376_RS31250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32160"
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_153218724.1"
                     /transl_table=11
                     /translation="MKTLYSPGDVAEQLGIQSSTLRKYADVLEKEGYTFIKNERGHRKY
                     RESDVMVFRKVINLKNDTDMTLENATKQIVSWHQGVEVLPLERHEIERYEEPDFNATTL
                     QMMLQDQNEFIEKQNELLQELNKRLVEQEQRFTQRESELLSAIQTIQESQMLIAMNTSE
                     DVAKNQGRDKMLMQTIREVQEVKKMIAASKDKKWYEFWK"
     misc_feature    11768..11786
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.95"
                     /tool="antismash"
     gene            11898..12602
                     /locus_tag="GE376_RS31255"
                     /old_locus_tag="GE376_32165"
     CDS             11898..12602
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263472.1"
                     /locus_tag="GE376_RS31255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32165"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_153218725.1"
                     /transl_table=11
                     /translation="MNVLITGGNRGLGLELVRVFHENGHTVFPVVKRKESLVFLSEMFT
                     DRCFPILTDISKDESITELTSQISLHTNYIDIVINNAGIPGKTYEIEKVNTQELMDLFN
                     IHCLGVVRTVQATLTYLRKSSNPRIINLSSRLGSLSKMSSGEFAKGKFSYSYRIAKAAQ
                     NMLTLCLDQELEDIPISVTAIHPGKLQTSIGSYDADMSPKGAAINIYSWLLNSKQDISG
                     KFFEPLVTEINW"
     gene            complement(12722..13282)
                     /locus_tag="GE376_RS31260"
                     /old_locus_tag="GE376_32100"
     CDS             complement(12722..13282)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020600.1"
                     /locus_tag="GE376_RS31260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_32100"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_153218726.1"
                     /transl_table=11
                     /translation="MNIGRQIQYFRKRDNMSQEKLAEKIHVSRQSISNWENERNYPDIH
                     NLLMMSILFNVSLDDLVKGDVKIMKEELQKSIFFKWSYIMLASMIILPISIAPVFYFFG
                     YYGLIIPFVLTILLMFSSLKVENIKKEHNLKTYRQIVDFIEGKPVSEVKPNIKDKLLKI
                     GLMLASALISYSLIYLGLSVFGR"
ORIGIN
        1 acatctccct taagtttgag gttaagttaa aggctaatta aagactacca aatttcttta
       61 taataacgtg attctaaggt gtaaaaatgt tttatcatgc taattcccct cattttttgc
      121 ccttttctgg ggtcccgccc tacacttttt attccacggt tcacttgacc acgagcctct
      181 acaccctcaa actcccaaag ataaccccag cgggcaaaaa ccgcccttct ggggccacct
      241 ctggtgagtt tgcatgaggg tgcccctcat ggtcaagtga ctttcgcggc ataaacgtta
      301 cggactcagt catcaaccct tttctaatta ttctcccatg tattcctatt acaaaatctc
      361 ttatatctaa attaatatat tgtataatat agttaaactt gattctatgt ggtatcacat
      421 agaatcaagt acatatgctc ccctccgggg agcccggttt atcgccttaa cggctttaaa
      481 ccgggaagat caagagcatc ccacaaatcg tcccgccgtc cttcgtcctg taagactcaa
      541 gcagggcaaa atacgcctgc cttggcatga taaaagctaa cgcttttcca tgcctgcggt
      601 cagactcatt catttttgat tctcgctgcg cttgaaccaa aaacaagctc ggcaagccga
      661 gtgaatggtc ttcaggcgta ttttgccctg cgggacttcc tggccagaac gcttcgcatc
      721 tgtccagagt ctttttctaa atcagctcac gcttcttaga aaaagacaaa taaaattgcc
      781 ctccggcatt ttatttgttt gatcaaaatg tcatgcttcg catcacattt tgaatgaaat
      841 taaataaaca tttttaaaca aatgtttatt taatacaaaa aacaagccat gtgaacatgt
      901 gtaaatatac acatgttcac atggcaaaga cacatagctt cgctatgtcc tcccaaataa
      961 ttaaatacac aaatatagca aagacaggag acattcctat ggccgcgcca cgaaaacata
     1021 taagaattct aaaaacaaaa gaagttgaag gcatggatat gctttggaaa actggaacag
     1081 ctaccagaga gcaaatggaa agagaatata acatcaaagg ggatcgccta aaaaaacttt
     1141 gtcatagcgg atatttagaa gaacgcacag gaaaaattgt tttaggagaa aaagggatag
     1201 aaaagtttaa aaaagaggga aaagaatatc aatataaaac cggtataaac aacgctaaac
     1261 acgatattcg tctctctgaa aaatacatct ctttaccgaa agaaacaaga gaaacatgga
     1321 aaacagaaaa acaattgcat tcagaagccc agaaagaccc cagatacgat gattttaaaa
     1381 aaagaatagt agagtcccat ccgcaaggaa aattccaacc aacgcccgat ggagccgtct
     1441 atagcgaagt gcatggcggt tatattgcca tagaagtaac cacacgtaac tacaaagaga
     1501 ttgatattca acaaaaacaa gaatttgcga agacattttt atccggatat gaacaactat
     1561 aggaggagaa aacatggcaa cacaaccaac atttatcgtt ccacctactt tttatacagt
     1621 acatgaaatt atgaataata cgaattcacc ctatatctta aacttacatg acattcagga
     1681 aaatccacat aagagaaaat taagtgatga attacattta ttcatcataa agcattatca
     1741 taatctattt acatttatgc tccatcatca gccctacatg ttaaaatctc aatttttgga
     1801 cctatttggc taccaaaaag gaaaaaagaa taagtctaga acacttggtt atgcaaaatt
     1861 aaaagagtta gaagataaac tgttaattgg ttctaaatcc ttttatggtg cggaatatat
     1921 ttatttatct tatcgagctt actattattt tggccaagaa aaaaaggtgc taccaattgc
     1981 accaggagag aatgtactct tccgccattt tactcgtatg gaagactatc tagaattaaa
     2041 taaacaatat acagacccaa cattattaat cgcattactt tgctctcacg agcattatta
     2101 tctattacat acagaaccct ttttcccaat ccaaccaata caagaaaaaa atacacgtta
     2161 ctttggagaa tacttcccaa acaacaaaat ccctaaaaat cttgatatgg aatttataac
     2221 aaactttctc ataaagacaa aacaagaaaa tagaggattg tggttaatca atgaagagac
     2281 acctgaaact caacttcatg tactcattac atttatcgat gaactcttta aaatccttac
     2341 tacacgttgt tctcttaaat tacttattaa tgatcaaaaa gaatctttac gaatcatagc
     2401 aaatattatc ccaataccag gaacatctta tatcacaatt aacaacctaa tctctgatat
     2461 ctatcatatt gaatttttat taaagatttg tttcccaaca gtcataacaa gctttacttt
     2521 tattgtgatg aaccaagatc atgaacgtga gttaaacaat gttataagta gaattactaa
     2581 acttagagaa aataaaaaga acttacaacc atggcaatct atgattgatc caaaaaacat
     2641 taaaatccgg aatacaaatc tatttagacg tttaaatttc aacgattcct acgaatctca
     2701 aagagaaaaa ccacttccac agtagccact tacttatcgt aagtggcttt cggtcttctc
     2761 cctacttttg aggtagaggc tggccggggt gtggggagac cccacgtatt gcgtatctaa
     2821 aaatagagta agaactaatt aaggaacgtt agagcaagcg atttggctcg tataatgagt
     2881 agctcaagcc gatatgccta catcccactt ttgagtgtat cggcttggca atgagagcaa
     2941 acgagccaaa aaaagtttgg caaggatgtt agaaaaaaga aaagtcttcg actttagccg
     3001 aggattgacg aggcgatttt ttttctaaca aaggtggaga tttttgagtg ttcttctcaa
     3061 aaatactacc tgttccttgc ggaactttta gctatttcta tatagagtga gaagggacaa
     3121 gcataccaaa gtggaaaatc cctagtaata tcaagggttt caagattttt cgtacttacc
     3181 gtttttggta agtaaactta ccgtttttgg taagtacgaa attaataaaa aaagtctact
     3241 aattaaagta gacttaaaat aatttgatag gtaaattttt aagttgttta gggacctttc
     3301 taaacatagc tttaagtgtt ggattcactt catccctatt tcctgaaaac ataacatgag
     3361 gatttataaa caaagcatat gccctaacat tattatcatc aacaccactc tcagaacgag
     3421 caataatgcc tttatcaatt aattttctaa ccaaaggact aactttcgtt tcatgtcttc
     3481 ctattttttt ggctaattct cgctgagtta atggtatttg ttctttagag ttaatatcac
     3541 taactaaaca attacttaaa aatcctacac acattgaaat atctaataaa aataccttct
     3601 cggcatttgt taaataatca atttcaaata aatactggat gttctgttga ataatctgaa
     3661 caaactttgc cttgtttttc actttacgct caggaactaa cttcattcct cttgaactag
     3721 cttttgcttg aagttcattt gctaaataca tctcttcttc tgacaaaatc tttgaatctt
     3781 cgatctctct caatcttgca tttttttcag cttgttttaa attgatagta tttgtcataa
     3841 cttttctcct cattcctagt atttacaact agtacagaga ctctttttat gctattatgt
     3901 aattagaaat ttataagaat aaaaaagcct cttatgtata gttgtagcgt cgcaactcaa
     3961 ttatatcata attggccttt tttgttataa ctaaaagggt ccttattact taggactctt
     4021 tttctataaa actaacacca tccaaccttg tatacgactt cagactactc tttatttatt
     4081 aaaaacgaaa acataaatat tatagaaaat gcaaaaaaac ttaaaagtgg taaagtaatg
     4141 aacccaaacc attctacctg aattgtagaa caatcacttt taatactaca aaaagaactt
     4201 ttcgtatgtg aaagttgaat tatatattgg tacatagcaa ttaataaacc taccaatgaa
     4261 aaacccctaa tataattctt caccttatca tcattagtca aaatagaaac taaaacaatt
     4321 aaaggtatag gatacataaa aattctttga taccaacata aatcacaggg tttcaaacct
     4381 aatatttcac taaaatataa actacctatt atcgaaagaa aagaaataca gaaagaaaca
     4441 taaagaaatt gtattcgagt tataatttta attttcatct taaaaacaca gccctcttat
     4501 aatatttcat caatttttga tttcaactcc gtaacattat ttaaaaaatc ttgttttaat
     4561 tccaatgttt ggatatcatt atataattca aaaacaatat cccatcttcg ggaaaaacta
     4621 tatagattaa ttagcaaatg tttaaagtga tctcccttag aatgaattgt gctacatgta
     4681 ttttcattag ttttatctaa agctacatgt aaagattcaa tgactgtatt tatcctattt
     4741 tctatgtcca agagcaataa agaagcttta taaaattcaa tatcgtaaaa atcgggatat
     4801 tcttcttcta tcacagaaat atattttatt aaagatttaa aatctttcaa ttcaaaataa
     4861 attttacaaa gtaacgaaag agattccatt aaaaatcttt tatcagattt aaatttatat
     4921 aaatctatag cacttaaaag tacactttca actaatcttt tatcagattt tgcttgattt
     4981 agttctcttg cataaaaaaa tagccattta ggattaccag gttctaactg aatcatttct
     5041 tttgttaata caagattccg ttcatttttt gctaccatat caacaatatc tggatcatat
     5101 ccatcatgat atacgtcaac ctttacagta atattgtctg gaatctcccc tccatcaaaa
     5161 attggctctt catgcacttt tccattaaaa gatatatttt gatttaaaga aaacattctt
     5221 cttgtatctg tatatatatc acctgtatat tcatgtatta taggactaat aacacattta
     5281 atattaaaaa attctattaa ccttgctatt cttttaaatt tactagtatt gttcttacca
     5341 aaataattat cagcatctat aaaataaatc caatcattgg ttacataatt aattaattta
     5401 tttctctgat acgaaaaatc atttttccat gattcagaaa taaccttgac ttgcgggaaa
     5461 ttttctctta ttatatctac tgttttatca ttagacattg aatctaaaac tactatttca
     5521 gaaaactcat taattatact gtctaaacat cgatgaatac atcgttcttc attataagta
     5581 ataatcccac aactaattga agggaaatca ctcttttcat ataaatcaat aatattattg
     5641 aattcatttt caaaattcaa atctatataa taatatctgt gaatatcatg tattaaacac
     5701 atatcctgaa aaagttgtga attaggattt ttctctttta ataattgata aaattcatat
     5761 aaactcatac aatcacccct ctttatttat acttttttga aaaaactctt tgagtttttc
     5821 aggacgatat ccctctaaac gttcagcttc ccttccatct ttataatgaa gaatagtagg
     5881 agttttattc aacttatact tatctaaaaa cgtctcatta taattttttt cttcctctat
     5941 atttaaagct tttaatttca atttattatt tttaataaat tcattaagat gaggttttaa
     6001 ctgctggcaa gatggacaac tagtcttata tacataaatg taaacatcat cattagaatt
     6061 tacactttgc tcatattctt taagatcaat atttgtataa aagttttttt ccagtgtttt
     6121 agaattaaaa atataaaaag ctgaacctat agatagtaat aatattaaaa aaacaaaaaa
     6181 ccattttttc atgatacaac ctctttttcc tcaattaatg ttttatcttt aaaaacaaat
     6241 ttacgattat aattttttaa catttgagag gtgtgagaaa taacaatcaa tgtgaaattc
     6301 atttttagta gattattatg tatcctctca aacttttcta aatcaacgtt acttaatgac
     6361 tcatctaaaa taagaacttc tggattatgc aaaatagcac gagctagtga aagtctttgt
     6421 ttttgccctg tagataagtt attagcattt tcatttaatt taaaatctaa attatcatta
     6481 agaacagata caacatccca aatttcagca acttcacaag ctgctataat ttcgtcgcta
     6541 ctaatttttt tacccataca caaattttct ctaatagttc ctttaaaaag aaatggattt
     6601 tcatttaaat aaataatttt ttctcttaat gtacttgtat ttattttatt tatatcaatt
     6661 ccatttacta aaatttgaaa cggtgacaca tcataaaatt taaccaaatt tttaaccaaa
     6721 gtacttttac ctactccact ttcacccaat aatagaatac gatcattttt aaaaattttc
     6781 atattaacat tttcaattac tctatttgaa ttatcataac caaaattaaa tcctttaaat
     6841 tctatatcgt gaatattagg tattttttta gtatcctttg cattaaaaac tgggtaatcc
     6901 actatatcta aaaaacgttc agttgcaatt acagcatgtt gaagttctgg ttgtaaactt
     6961 attaaccttt ctaaagaaga taataaaaac atggccaaag aattaattaa taataatgta
     7021 cctaatgtaa tagaatcatt aagtacttgc tgagtaccca cccataaaat aataatagaa
     7081 aaggaagaat gaattaataa tcgaactgca ttattaatat ttacagtctt aaattctttt
     7141 aatgctgaat ttatttgttt cttaaaaaaa acatcgaatg aatctaagaa atacttctct
     7201 ttattaaacg aatatatagt aggcatattt ttaataaatg gaactaaaaa agaagtagtt
     7261 tttgcttcat taaccattaa ttctcgactt cttttgttta aaatttcata aaacattatt
     7321 gcacataaag tcaataataa aacaggtact aacacggtaa aaaacaatac atcattcatt
     7381 ctataaagaa aaacacttat tcctaaaatt ataaatatat caagtattcc tgtaactaca
     7441 ttgacactaa atgcatttct aatatagtga ctatcccgaa aacgtgaaat aatttcacca
     7501 tcatccctat tttcaaaaaa cttaataggt aaatttacaa ttttatcaaa atatttttca
     7561 gctaaccctt tatctaaatt atatgaaatt cgaacaacaa aataatttct aatataatca
     7621 aatagtactt tcacaaaatt tataactaag aatactaagc taaattgaat taatgtagta
     7681 tctaaacgca ttggaataac aaggtctgtt aaaaacttaa caaaaaatga aagaataata
     7741 gctattccta caaccaatac agataaaata aaagttaaag tgaacaaaaa tttattttct
     7801 ttcaataccc ctttaaaaag attagcttta ttatatcttt gattaaaaat atcattttta
     7861 ttttgatcta cagattctac aactaaaagt actccagaaa atacatttga aaaatcctta
     7921 attaacattg ttgaaatttt attttttagt ggatcactga ttactaattt gttccccttt
     7981 attgaataaa taacaatgta atgcttttct ccctctctct ctattaaagc aatagatggt
     8041 tttttaatat aatttaaagc ttcttttaat ttctcctcat taattcttaa agatttataa
     8101 gttatacctt taaaattccc taataaaaaa gttaaatctt ttaaactata ccctttctta
     8161 tctaccatta aatcccttaa ataatcaata ccataattaa aaccatagta tcttaataaa
     8221 gaagagacac aagctaagcc acaatcatgt tcattatgct gtatagtatg tataaatttg
     8281 ttttttttca taaaataagc ttcctttatg tatttaatca aaaagaaatt tcattaaaaa
     8341 atgaaatttc tttttgatta aatataattt atttattaca catttttcga tatgtattac
     8401 attgaccata tgcctgtgta gcagtaccac cacaaccaat tcttccgccg cttgaacatt
     8461 gagctaataa tgctgcacat tgtgcccaac ccataccacc atgtcctgta tgtttatcta
     8521 attcttcaac ttttaattct ttaaataaat ctttcataat atctcatccc ttactaattt
     8581 tatttacaag caactatatt gtaaacgttt actagtaaga ttttacaata ttaagcaaac
     8641 gtttgcaata gttttttctc aaaaaatacc aaacttaaga ttatttgaaa gtttagggga
     8701 tataagttaa ggatttcatc caatataatt ccatcaaaaa aagcaaacgt ttgcaaaaat
     8761 ttaaatttaa aactattaaa acattatgaa atatagttaa gccttttcac ctaatataat
     8821 tccatcaaaa aaacttttta attcagaaaa cttttctaaa tctcccattg ttgaaaaaac
     8881 ttcttcgccc tttttatgac aaataacaat tgaaggaatc ttcttaaata aaccaacagt
     8941 ttgcttctgt tctttaaact ctataatttc tccataatca atttcaaaaa gaattttatt
     9001 cattttcatc gtttccgtat aaaaaattat tctcctattt gttgttaata ataatccgaa
     9061 tacatcagga gaactagata tattaccgcc taacatagaa agagaagtca aatctttata
     9121 tttataataa gctttaattt gatttctcac cttttcattt tcttctaata aaatttgttt
     9181 taacatatca agtctctcct tatataaaaa taaaatattg ataatatttt ctgaaatcct
     9241 tacattacca tattatcatg agacaaaaat gtatcaacgt atcatttgac ataatcttga
     9301 ttaataacca gattcttaat atatatttta aagaaacaaa aaacattttt ttccatctta
     9361 gtcacattat atattatcat cacaacgtct aaatatgtaa ttttgcatat cccaaatatc
     9421 aatacaaata atcctagtag agattactat atccaatgta aacttttcaa tattagttta
     9481 cattcaaata cccttaaaca ttgacttctc atagattctc ttgatatcct caatttatat
     9541 aataaaagta tacattcaaa atactataaa taaagaggtc ataaaaacat gaacaaaaaa
     9601 ccacatctca ttgatgtcca acctatccgt aacaaagaac aaatcgaaga tatgaaatgg
     9661 gctcttaaac gtcattgttc tgaaagagac tatattctgt ttcttattgg tattcatacc
     9721 ggactacgcg taagtgatct actgcaactt gaaacacaaa ccatagtaaa actgaaacaa
     9781 aaaaagagaa aagaatttaa gatcaaagaa ggaaaaacca aaaaagaacg cgtgattaat
     9841 cttacttcta tttttgatga agtctattca tatgcccaaa ccctagaaaa tacctggtta
     9901 ttcccttccc gtaaagggaa tcagcccatt agtaagatcc aagcttatcg acaattacaa
     9961 aaagccagag atttttccgg tgtagaatcc attggtactc acaccatgcg aaaaaccttc
    10021 ggctattggt tctataaaca aacaaaggat gtagctatgt tgcaagacat actcaatcac
    10081 agtacaccac aaattacact taaatatatt ggtattaata aagaagagaa agataatatc
    10141 cttgatacat ttcaaattta atctttttta tttccattaa tttcaatata ttaactttac
    10201 caaattcgaa aagttttaat tgatgaagtc acatcattaa aatcttggtg aatattaaaa
    10261 agataagatg tgtttccaaa gttttcaagt attttaactg gatgtccata gtttatatct
    10321 gtatacaaaa ttactaaagt tctaggcgca acacttacag aagaaattct atcattccaa
    10381 ggatccccca catatgacaa tctctctccc gaatttaaag tgaaagacat accctgacca
    10441 tttatatgtt cataaaatct attgatagat tgccgaacat catcgttata ctcatatgaa
    10501 ttataatgat acatgaatat cactcctata ctttgttttt tagtaatatc atattaccgg
    10561 tataaatttt agatgattgt ctatgtataa ttgtgctaat gactaacagt acaataaaaa
    10621 ataccaaaag gtacccatgt ctattaagta tgagtacttt ttggtatttt tttatcattt
    10681 aaaaattaat tccttattcc tttactacac aattcatttt taaataaaaa aatcctatca
    10741 acaaaataaa aatttcataa actacaaaaa ataattaatc aaatttaaaa gggttatttc
    10801 caaaactcat accatttctt atccttactt gctgcaatca tcttcttaac ttcctgaact
    10861 tcccttattg tttgcatgag cattttatcg cgtccctggt tcttcgctac gtcttcgcta
    10921 gtattcatcg ctataagcat ttgggattcc tgtatcgttt gtatagcgct caataactct
    10981 gattctcttt gtgtgaatcg ctgttcttgt tcaactaaac gtttgtttaa ttcttgtagt
    11041 aattcatttt gtttttcaat gaactcgttt tgatcttgaa gcatcatctg tagcgttgta
    11101 gcgttgaaat ccggttcttc atagcgctca atttcatgtc gctctaatgg caatacttca
    11161 acgccttgat gccacgatac tatttgtttt gtggcgtttt ctaatgtcat atctgtgtcg
    11221 ttctttaggt taataacctt acgaaacacc attacatcac tttctctata cttcctatgc
    11281 ccacgttcat tctttatgaa agtataaccc tctttttcta gtacatcagc atacttacgt
    11341 aatgtactag attggatacc taattgctct gccacgtcgc cgggactata taacgtcttc
    11401 atagcgtctc acctcaaaaa tatattcgct atagcaatga tataaccttt tccctaataa
    11461 ttcacgtatc actgttttaa aattcttaac ttctataatc tttacatgag taatttgtat
    11521 aatttgcatt acaaattcaa tattaaaaat acatatcaat tttaatattc agtacttata
    11581 aaatctcgat accagcatca aaatcttttt ctatatacaa gattttatgc aaaaaaagaa
    11641 tagcgggaat ccctagaatc ctcgatatat caaccatgta taaaatcatg catatcaatt
    11701 ttactagaaa ataagaaaga accctataaa aacaaggatt ctttctttat acttgctacc
    11761 gataatgata cgttatgtta accttacatg tatagaatat acatcctgta tataaagtaa
    11821 tttcagtaaa aattatcaat ataaggtgat atattaagaa gggaatctac gacattgatt
    11881 taggaagggg aattgtattg aatgtgttga ttacaggcgg aaatcgaggt ttaggtttag
    11941 aattagtacg agtatttcat gaaaatggtc atacagtgtt tccagttgta aaaagaaaag
    12001 aatccctagt tttcctgagt gagatgttca ctgacagatg ttttcccatc cttacagata
    12061 taagtaaaga tgagagtatt acagaactaa cttcccaaat cagcctccac acaaattata
    12121 ttgacatcgt tatcaacaat gctggtatac caggaaaaac atacgagata gaaaaggtaa
    12181 acacacaaga gttaatggac ttatttaata tccattgctt aggagtagtt aggactgtac
    12241 aagcaacttt aacgtattta cgtaaatctt caaatcctcg tattattaat ctatcatccc
    12301 gattaggttc actatctaaa atgtccagtg gtgaatttgc aaaagggaag ttttcatatt
    12361 cctatcggat cgcaaaagca gcccaaaata tgcttacgct atgtttagat caagaattag
    12421 aggatattcc tatttcagtt acagctattc atccaggaaa gttacaaaca tccattggat
    12481 cttatgatgc agatatgagt ccaaaggggg cagctattaa catatattct tggcttttaa
    12541 attctaaaca agatatatcc ggcaagttct ttgaaccttt agtgacagaa ataaactggt
    12601 aaagtgtgta cattgatttc attccatatc tagagggaat aacttccggt aacgaaaatt
    12661 atgtaaacac aaaagtcctc cagttaaaca atccggagga cttttaatag ttttctaagt
    12721 gttatcttcc aaatacactc aatcctaaat aaatcaaact ataactaatt aaagcacttg
    12781 caagcatcaa acctattttt aataatttgt cttttatatt aggctttact tcacttacag
    12841 gttttccctc tataaaatca actatttgac gataagtttt taaattatgc tcttttttta
    12901 tgttttcaac tttcagtgat gaaaacataa gcaaaatcgt aagcacaaaa ggtattataa
    12961 gaccataata accaaaaaag taaaatacag gtgcaataga aataggaagt ataatcattg
    13021 atgctaacat aatgtaactc cacttaaaga aaattgattt ttgaagttct tctttcatga
    13081 tctttacatc tcctttaact aaatcatcaa gagagacgtt aaagagaata ctcatcatta
    13141 gtaaattatg aatatctgga tagttccttt cattctccca gttagaaata gattgtctgg
    13201 aaacatgaat tttttctgct aacttctctt gggacatatt atcccgttta cgaaaatatt
    13261 gtatttgtct accaatgttc atctat
//
