LOCUS       NZ_CP045606            10330 bp    DNA     circular CON 16-FEB-2024
DEFINITION  Bacillus cereus strain SB1 chromosome, complete genome.
ACCESSION   NZ_CP045606
VERSION     NZ_CP045606.2
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5461537)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Bacillus cereus SB1 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5461537)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2019) Department of Physiology, Anatomy &
            Microbiology, La Trobe University, Kingsbury Drive, Bundoora,
            Victoria 3086, Australia
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 5461537)
  AUTHORS   Batinovic,S. and Petrovski,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2019) Department of Physiology, Anatomy &
            Microbiology, La Trobe University, Kingsbury Drive, Bundoora,
            Victoria 3086, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 46x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/16/2024 03:34:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,375
            CDSs (total)                      :: 6,221
            Genes (coding)                    :: 5,991
            CDSs (with protein)               :: 5,991
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 140 of 230
            Pseudo Genes (internal stop)      :: 70 of 230
            Pseudo Genes (multiple problems)  :: 57 of 230
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:27:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2572000
            Orig. end                         :: 2582330
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045606.2.
            On Dec 19, 2019 this sequence version replaced NZ_CP045606.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Steve Petrovski, La Trobe
            University, Bundoora, 3086, VIC, Australia.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    285..299
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            375..1823
                     /locus_tag="GE376_RS12940"
                     /old_locus_tag="GE376_13205"
     CDS             375..1823
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_GE376_RS12940_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="GE376_RS12940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13205"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471611.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 5.6e-28, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        810..1781
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GE376_RS12940_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="GE376_RS12940_Aminotran_1_2.1"
                     /locus_tag="GE376_RS12940"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1828..2742
                     /locus_tag="GE376_RS12945"
                     /old_locus_tag="GE376_13210"
     CDS             1828..2742
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="GE376_RS12945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13210"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_003270605.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 8.5e-22,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.3e-13, bitscore:
                     49.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.3e-08, bitscore: 33.0,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 4.5e-53,
                     bitscore: 178.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.3e-05, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     misc_feature    complement(2765..2777)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2816..2921
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3049..3738
                     /gene="tenA"
                     /locus_tag="GE376_RS12950"
                     /old_locus_tag="GE376_13215"
     CDS             3049..3738
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="GE376_RS12950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13215"
                     /product="thiaminase II"
                     /protein_id="WP_000144507.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGEHLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAENVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDK
                     DVKDVIG"
     misc_feature    3901..3914
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.67"
                     /tool="antismash"
     gene            4189..4452
                     /locus_tag="GE376_RS12955"
                     /old_locus_tag="GE376_13220"
     CDS             4189..4452
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="GE376_RS12955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13220"
                     /product="DUF3937 family protein"
                     /protein_id="WP_153217923.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFIISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5330
                     /locus_tag="GE376_RS12960"
                     /old_locus_tag="GE376_13225"
     CDS             5001..5330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="GE376_RS12960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13225"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071355.1"
                     /sec_met_domain="TIGR03601 (E-value: 5.9e-39, bitscore:
                     130.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYQSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            <5470..5604
                     /locus_tag="GE376_RS31470"
                     /pseudo=""
     CDS             <5470..5604
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="GE376_RS31470"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYLPTKNIAIINGYIQSFK"
     misc_feature    complement(5764..5782)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5814..6299
                     /locus_tag="GE376_RS12965"
                     /old_locus_tag="GE376_13230"
     CDS             5814..6299
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="GE376_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13230"
                     /product="hypothetical protein"
                     /protein_id="WP_003270601.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     ENYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    6606..6619
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6611..7312
                     /locus_tag="GE376_RS12970"
                     /old_locus_tag="GE376_13235"
     CDS             6611..7312
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="GE376_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13235"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_000736206.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKML
                     GQTNLDITTFV"
     gene            complement(7351..8460)
                     /locus_tag="GE376_RS12975"
                     /old_locus_tag="GE376_13240"
     CDS             complement(7351..8460)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="GE376_RS12975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13240"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_153217925.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIEKSTFAGYKAFINQHVIPSIGMVA
                     LHKLNVIHIQKCYKTAIDKGIANNSILLMHRILKSALNLAVKQNIISRNPADFAEIPKK
                     EKTPIQTWTEEEVKKFLVHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIAALKKHKILINKEKLRFGSAYLDQDLINC
                     NEFGRIIKRAHFRKSFIRMTHKVGIKEIKFHDLRHTHATLLLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            9007..10158
                     /locus_tag="GE376_RS12980"
                     /old_locus_tag="GE376_13245"
     CDS             9007..10158
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265310.1"
                     /locus_tag="GE376_RS12980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GE376_13245"
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_153217927.1"
                     /transl_table=11
                     /translation="MWKALNQIEKELCAAGIRKNKLANYWGVKPSTVTKVFKGNTDMSF
                     GFLSKTVILLNKGIQVQESLLTDYINITKPKSENLREAMEDLALRGKFNLLINIINSES
                     QSKVAENREFANVYRIIYRRYIGEIDAIQYHKALSLESKSIRTLEMEVLIEILLCQAQY
                     QSGNFTSLNERLKSLEIKLNKISNRYIRECYKLRYKEAIAVTSLQGGEVIEARHVSMEL
                     LDDLEWDNFFSFPKVNAYLKLGESYIFSANEYEKAKYYLEKTLEVLGDSKINGIERKRK
                     MVQHTLSFLKIHHDKEISSIDVVHPGELAYLRIKQGKRIEARKLLIQLKEKNGRLTDIQ
                     TAYLGLTYEGTKKEELMKRSLLMCQKSGNIFYSNLPKIHLGLI"
ORIGIN
        1 cacgttacaa aacagcaaat tcgaaatgct aaggaacaac ataaaattat taaattaata
       61 gcatcagcat ataaggatga gggtggcaat gtcaatttaa atgttgaacc gtgcgaaata
      121 gaaaaagatc atccgttagc aaaagtaaat gggaccgaaa aaggaattac gttctttaca
      181 gatacgatgg gacaagttac tacaattggt ggggcttcta atccacgagg cgctgcagct
      241 gcagctttaa aagatgtaat taacttatat cgaaaagatt tgtaagaccg taccgccctt
      301 tataaaaaat acatatgtcg tggacctttt gagaatgaaa taaatatgca aaaatgattg
      361 cagggggtaa ggaattgttt aaggatttta aagttgttaa agatcgtccc gtttatattc
      421 aattgaaaga ttatttgaaa aagatgatta tgaaagggca tttgctcggg gatcaaaaaa
      481 tcccatcaac aagggaacta agtgaattac taagtgtgag tagaaacact gtactcgctg
      541 cttatgcaga tttagagcaa gaaggactca tttatgcagt taaaggaaaa gggaactttg
      601 ttgcgaaggt ggatatatct aacacttcgt ctgttgaaat agattggaaa aataaactta
      661 atacagtgac cgcattagcg gacgaattag atttaatgaa acatggtgtt cgttgggaaa
      721 agggaatgat tgtttttaat agtatagccc cggatgagaa gctatttgat gttgaaaatt
      781 tcaaaagagc ttttcttact cgtatgtcca ttgaagggga catcgtattg aactacggat
      841 atgcaaaggg atatagaccg ttaatgaatt atttacttca ttacatggaa atgaaaggtg
      901 tagacatttc gaataaagat attctaatta caaatggatt tacagaaggg ttggatattg
      961 tactctcttc tttatcaaaa aaatccggtc gtgtcatttg tgagaatcca actcaccatg
     1021 ctgcacttaa gcttttccgt ttacatggac ttgaagttca tggaattgat atgcatgagg
     1081 atggtattga tacgaatgaa gttgaaaaaa gtttgcgtga aaaagatttt gattttgctt
     1141 atttaattcc ttcttatcat aatccaaccg gtattgttac gagttcagag aaaagaacgg
     1201 aattgatgag gttattctca aaatacaacg ttcctattat agaggacgga tttaatgaag
     1261 aattacgtta ttcaggttca catttagctc cattattaac ttttgctggt gctggcaata
     1321 atgtgattta tattagtagc ttttcaaagg tacttttccc tggtttacgt gtagggtgga
     1381 ttattgcaga taaagagctg attcattatt tagaaagcgt aaaaagagca agaacgattc
     1441 acacatctac gctagatcaa gctgttctgt ttcaatattt acatgaggga tattttgaaa
     1501 aatatttaaa aaaggcaaga tctgtttata agaaaaaata tgagttagct gtcggggcat
     1561 gtaaccagta cattcctttc aaaagaatga ctggagatgg tggactgcat ttatttatag
     1621 agctagaaga gcatatgaac gcccgcacac ttttacaaaa gtgttatgag aaaggcgtta
     1681 cgttttcacc tggagatgtt ttttactctg atggagaagg agcgaacact ttccgattag
     1741 ggttttcgcg cttgaaagaa gaagaaatag ttcggggaat taaaataatt ggtgatacat
     1801 taaaaaatga aaattggagt tgagaaaatg aaaatcggtg ttattatggg cggggtatcg
     1861 tctgaaaaac aagtttcaat tatgaccggg aatgaaatga ttgctcattt agataaaaat
     1921 aagtatgaaa tagtcccaat tacattaaat gaaaaaatgg atttaattga aaaagcgaaa
     1981 gacattgact ttgcattgct cgcattacac ggaaaatacg gagaagatgg gacagttcaa
     2041 ggaacactgg agagtttagg gattccttat agcggaagta atatgttatc tagtagtata
     2101 tgtatggata aaaatatttc gaaaaagatt ttacgttatg aaggtgtaga aacaccagat
     2161 tggattgaac ttacaaaaat ggaggatcta aatcttgatg agttagataa gttaggattt
     2221 ccattagtgg taaaaccaaa ctccggcgga tcgagtgtcg gggtgaaaat cgtttacaat
     2281 aaaaatgaat tgatctcaat gctagaaact gtattcgaat gggattctga agtagtaatt
     2341 gagaagtata taaaaggtga tgaaattaca tgttctattc tggatggaaa acagttacct
     2401 atagtttcaa ttcgacatgc agccgagttt tttgattaca atgcaaaata tgatgatact
     2461 agtacagttg aggaagttat cgaacttcca gcagaaatta atgaacgtgt aaataaagct
     2521 tcactggctt gttataaagc attaaaatgt agtgtttatg caagagttga tatgatggtg
     2581 aaggacggaa ttccatatgt aatggaaatt aatacattac cagggatgac acaatcaagc
     2641 ctattgccca aaagtgcaga agcagcggga attagttata gcaaactatt agatatgatt
     2701 attgaaacat cattaaaagt tagaaatgaa gaagggtttt aaggagatta tataactaat
     2761 tcttttgaca gtttcacgaa gtatgctatg atgaatacat tataaaaaca aaatatttat
     2821 ccactagggg ggccttttat aggctgagat caaatgtgat tttgagactc ttagtacctg
     2881 atctggttaa tgccagcgta gggaagtggg aacgacgttg ctatttaatg agtaaataaa
     2941 tactgtcaat ttcactttct ttacgcctgt aaagaaagtt tttttatttt acagctttaa
     3001 taattgtgga taaatactta tattttatac attattggag ggatttaaat gactttttca
     3061 caatcattac gtaaagaagt agattcaatt tgggaagcga gttttaacca tccttttgta
     3121 aaaaaacttg gtgaaggaac attagattta gctagttttc gctattatgt gcttcaagat
     3181 tcatattatt tgagtcattt tgctagagta caaactttag gagctgcaaa agctcttgaa
     3241 ttagagacga cagctcgtat ggcacatcat gctcaaaata catatgaggc cgagttatct
     3301 ttacatgaga attttgctaa aaagctaggg ataacaaaag aagaaaaaga taattttatt
     3361 cctgcgccaa ctgcatatgc gtatacttca catatgtatc gtgctgcgta tgaagggcat
     3421 ttaggagata tcattgcagc aattttacct tgctattggt tgtattatga aattggtgaa
     3481 cacttaaaag agtgccagcc ggaagagcca atttataacg aatggatttc tgcttatgga
     3541 tctgattggt tccgtacgct agttgaagaa caaattacac gtttagatac tatcgctgaa
     3601 aatgtaacag aggcagaccg caaacgtatg aaacagcatt ttattattag cagtcaatat
     3661 gaatattcat tttgggaaat ggcttataca ttagaaaagt ggccagtgga taaagacgta
     3721 aaagacgtaa ttggataagg aatagaagta atgttataga taggtatgga taatatctat
     3781 ctatatattt tttttgtcaa atttgtacaa agttgatagt actgcagtta tagcgaaatg
     3841 acatatcgat aagttagttt agttgtctac gcattaaatg gataaactac tgtcacattt
     3901 attttacaat aaaacggata tgttactaag ctaacttcag atacacaacg taatgagatg
     3961 aaattttacc ttgaacgtaa agcttggagg tatgaagtta gataagaaaa gaaacccgca
     4021 ctgttagctg aacggagttg ttttttcttt gcaggaattt ctaaattacc atgaagtgct
     4081 gttaacacaa tatgcatatt tgcatattgt gttttttttg agttcttttt gtgttaaaat
     4141 caaatatagg gatatggaaa tataaagaag taaaagcagg aatatccaat gaattttgag
     4201 aggtgttata tgtttacaaa taagaaatta attcgatttg gtttatcgtt gtttgtattt
     4261 ttagggataa ttaattttat aatcagctat ttccaaacat atcttgaaac agcagcagat
     4321 attaaatggg taattccgga aatttggaaa actattttac tagatgttcc tcaaggtata
     4381 cttgttcttt taggcgcaat tgctttatat gatttcacaa aagaggcatc acaaaaagac
     4441 gcatcaatct aagtgtgtct ttttctatta ggaatttcat agagagtttt gctggagaaa
     4501 tagaatgaaa agccctaata agggcttttt tctttgtagg aatttcttat ccgtcatata
     4561 aaccggtcac taggagatgt tatagcgaaa cgcatttaca ttacagaaaa atctaagcgg
     4621 cagacgcaaa tatataggat atggcaacat aattattgag aaattaattg aatttagtag
     4681 gtatgaagat atgacgctag aattcatatt ttttaatttt tatttatccc gcattaacgg
     4741 gctgttcgta aaagcctgat tggtgagggc taataatcag cgggggaaga actccccccc
     4801 actgattaaa gtttcacttt ataaaaatga gcccatagca aaaataggta tatgaacaag
     4861 gtgcatcatt tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 gctattttta cttagatgta acgaacagaa atatagggaa gcatcaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga actacaagcg ttaagtctta
     5041 atgattacca atctggaaat attgtctatt gggaccagca aaatcaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtggcg gttgtggtcg ttgtggtggt tgtggtggag
     5161 gtcgttgtgg cggatttcgt tgtggtggat tccgttgtat tggttgcttc ggttgttttg
     5221 gttgcggagg ttgtggaggt tgtggcggct gtggtggttg ctctaactgt tttgatggtt
     5281 ttagtggtac tactgatact actggtacta ttataacgta tgaatattga ttgcatttat
     5341 cctgctattt gccgggcagt aagaccctaa aaaaattcag cgagagcaaa gtggcggact
     5401 acccataaaa gcccgattgg tgaaggctaa ttaaagcttc actttatcaa ttaaaaaaca
     5461 ttagttttat atatgtactt gccgacaaag aatatagcca ttattaatgg atatattcaa
     5521 tcattcaagt gaaagagtaa caataagata tatagatgta atcaagaggt gatggataaa
     5581 gcaatgacta gatttaaaat ctaatcattg ctcttttctt atggttttat catcaaatat
     5641 ctgtctagtt tagggattga tttcttctag ctaatgaatt tattacaact ttgtaaggtt
     5701 aatgtaatgt taatcgatag taaatgaaga tgcttgagtg taatttaaat aagattaaaa
     5761 agtactgttt atctataaaa taatcgggta aataatatgt aattaggaag tgtatgaata
     5821 tgattgaact aaaatctatt atccattcgt ataaactaaa gagaaaaata gctaaagatt
     5881 tatacggaaa aagagatgaa ttgacactgt tattaaatga atttaataaa atgaaatgta
     5941 cagtaacatc tgaaaagaag aaaaacaata tattatctcg tttgcaacta atttatcaaa
     6001 atatgaaatt agataagcag tatcctctat cagttgcttt tgatagtaaa ttattggagc
     6061 gattagaaaa agaacctcta catactattg aggatggtgt aacatgttta catctaatgt
     6121 tagatgagaa ttatgagaaa ataaaacaat atggttcgag tacaagtagg tcatttgttc
     6181 cattatcgca gtcttctatt tgtcttgctg attgtatttg tttaacagga gttgtagtag
     6241 gtttactagg agcaatttca tttgggggat tactgttatc tatatgttca attacataaa
     6301 ggttgatatt tgtaaaaagg gcaccgaatt aaggtgttct tttttggttt ttttgtaaag
     6361 ttatcgtaaa tgataagtac aatgcattag atgtaatgta taattcgaat gtattgaaat
     6421 acaaatataa tagaggtgtt atacatgata attactttga atttttaaaa gagtcggttt
     6481 gataataatt tatgtaaata aacagttttt acgtttcttt taaatacttg gtttaaatgg
     6541 attagattaa gtaactattg ttctagcttt ttgataatca agacaactga agaaaggaag
     6601 gaatcatttt atgaaaaaat taaggctgct aacatttgaa aatatagtag aacccctttt
     6661 aaatgaaaag gtatcattta tatactttcc tattgaatgg ctggacatcg tagagataca
     6721 ttataagaca tttttattaa cgagtaagtt gaaacgtttg aatgaaagat tgtatgatat
     6781 gttttcggat atattgttta ttcagcataa cccgtacgta ttaaatgaaa atacaccatg
     6841 gattgtatcg aaagaaccta ttagacaaga gcaactcgat tatatttttc aaagttggta
     6901 tgagattatt catgattgga aaccaaatag attagtagaa ccaccaaaat atgaatggca
     6961 atccgatttg atttctaatt tgccagtact acatgataat gaaacgtatt ctaagtgggt
     7021 gcccgcttta atctcacata ttttttgtga gcgtcctata tatttagaaa atacaaatga
     7081 agaagaaatc tatttttccc ctcttagatc acaaaatatt tgtgaggcga tgtcagggcc
     7141 gataaaagat gaaaaaacac aagatttttt ctcctatgta tatcgattcg aatgcataac
     7201 ccgcggtggt gagaacgctc cattattaaa tatttcaata gggattcgga gattttatca
     7261 agaatataag atgttgggtc aaacaaacct tgatataaca acgtttgttt gacttgtttt
     7321 tataaaatgc aaacattttg caaacataag ttatccaaag atacttttac cgaagttttt
     7381 aacagcttct tcttgcatat tcggtaaaac atgagaatag acacttaatg tcattgaaat
     7441 atctgtatga cctaatcgct cactgatgat tttagggtta actccttgtt tcaatagtag
     7501 agttgcgtgt gtatgtctta aatcatggaa tttaatttct tttataccta ctttgtgtgt
     7561 catcctaatg aaactttttc tgaaatgtgc tctttttatg attcttccaa actcattaca
     7621 atttattaaa tcttgatcta gataagcaga accaaacctt aatttctctt tattgattaa
     7681 aattttatgt ttttttaagg ctgctattgt ttcgttaggt acaggaattg tgcgttttga
     7741 tgaatttgtt tttgcagttt ttttgatttt attgtcatga ccagatgttt gatttattgt
     7801 aacagtatgt ttttcaaaat caatgtcctg ccatcgtaac cctagaactt ctcccagacg
     7861 catacctgta gttattgcaa gtagataccc aatgtgatat cgtgattctt gtgaatgaac
     7921 taaaaacttt tttacttctt cctctgtcca agtctggata ggggtttttt cttttttagg
     7981 tatctcagca aaatctgctg gatttcgaga aataatattt tgttttacgg ctaggtttaa
     8041 agcgctcttt aaaattctat gcataagcag aatagaattg tttgcaatcc ctttatctat
     8101 cgcagtctta tagcattttt gaatgtgtat aacatttaat ttatggagcg caaccattcc
     8161 tatactaggt ataacatgtt ggttgataaa tgccttatag ccagcaaagg tactcttttc
     8221 aatgctcata cttttaattt ctagccaatg atttagataa tcctttaacg taactttaga
     8281 tggttctata aaagttcctt cattcaattc tgtaatcttc tttgctacat cggcctgtgc
     8341 ttcttttttt gtcttatatc cagaaaacca cttctgtctt ctttttcctg tctctggatc
     8401 aggaccgata tcaataacga tacagtattt atttcctctt tttcgaatat gtcctttcac
     8461 ttaaaacact ccttcatttg ttttgaatca tgttgtataa gtctggttgt aattttgctg
     8521 ctatgaaaat tacatgtttg gacatatcag cgatggatat attttaccat ataaaaataa
     8581 attcagttat agtgtagggg gattcatttg ttgttaaaca atagtaaaat gtctactccg
     8641 attattaaat gcaatattgc atattttttt aggaagtcta caaaacaagg aatatattaa
     8701 tatttttaac tttctcaagt attttcagaa tatgagagaa tagatgtttg ggttatttgt
     8761 gatagaatat tcttaataat ataatttgaa ggaacaaaaa agacccatag cgtgtgtaat
     8821 tgtgctggga acacttttac accgtttgcc ctaattgtag tagggaaaac atttgccatg
     8881 agccttttag ttacgactat gcgtaacata tacggctagt ataacacaac ttttagatgt
     8941 aattcatcat taaggtgcgt tttcatgata gggaagtgtg tcttgttcca ataaggggga
     9001 caaaacatgt ggaaagctct aaatcaaatt gaaaaagagt tatgtgcagc tggaataagg
     9061 aaaaataaac tagcaaatta ttggggagtt aagccaagta ctgttacaaa agtttttaaa
     9121 gggaacacag acatgagttt tggctttctt tctaagacag tcatcctatt aaacaaaggc
     9181 atacaggttc aagagagttt attaacagat tatataaata ttacgaaacc aaaatcagaa
     9241 aacttacgtg aggcaatgga ggatttagct ttaaggggaa agtttaatct attaattaat
     9301 attataaata gtgaatcaca atcaaaggta gcagaaaata gagaatttgc taatgtgtat
     9361 cgaattatat atagacgata tataggtgag attgatgcta tacagtacca taaggcatta
     9421 agcctcgaaa gtaaatcaat aagaaccttg gagatggaag tgttaattga aattttatta
     9481 tgtcaagcgc agtatcaatc gggtaatttt acttctttaa atgagcgatt aaaatctctt
     9541 gaaataaaac taaataaaat aagtaatagg tacattcgag aatgttataa attacgatat
     9601 aaagaagcca ttgcggttac ttcattgcag ggtggtgaag tcattgaagc aaggcatgtt
     9661 agcatggaac tattagatga tcttgaatgg gataactttt tttcttttcc aaaagtaaat
     9721 gcatatttaa agctaggaga atcgtatatt ttttcagcga atgaatatga gaaggcaaaa
     9781 tattatttgg aaaagactct tgaagtgcta ggggatagta agattaatgg aattgagaga
     9841 aaaagaaaaa tggtgcaaca tacattatca tttttaaaga ttcatcatga taaagaaatt
     9901 agtagcatag atgttgttca tccaggtgaa ctagcatatt taaggattaa acaaggtaag
     9961 aggattgaag cgagaaaact attgattcaa ttaaaagaga agaatggaag gttaacagac
    10021 atacagactg catatttagg tttgacatat gaaggtacta aaaaagaaga gttaatgaaa
    10081 cgttctcttt tgatgtgtca aaaatcaggg aatatatttt attcgaattt accaaaaata
    10141 cacttgggtt taatttgaat aattggtata attgtcttcg gaaagaggtg aaataatgaa
    10201 aaaaataatt acaatcattc ctgcactatt attagctgct acattattca ttaacactga
    10261 ttctataaaa gaaaaaccta gtacaaatga tttaaaacct acaattcaac acatgatggt
    10321 tgaccctggt
//
