LOCUS       NZ_CP045480            10810 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Amycolatopsis sp. YIM 10 chromosome, complete genome.
ACCESSION   NZ_CP045480
VERSION     NZ_CP045480.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. YIM 10
  ORGANISM  Amycolatopsis sp. YIM 10
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Complete genome sequence of Amycolatopsis sp. YIM 10, isolated from
            a rare earth mine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2019) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009429145.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 04:03:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,775
            CDSs (total)                      :: 9,711
            Genes (coding)                    :: 9,634
            CDSs (with protein)               :: 9,634
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 22 of 77
            Pseudo Genes (incomplete)         :: 65 of 77
            Pseudo Genes (internal stop)      :: 1 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:01:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6505291
            Orig. end                         :: 6516101
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045480.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10810
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10810
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10810
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5810](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5810
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            28..1389
                     /locus_tag="YIM_RS30705"
                     /old_locus_tag="YIM_30935"
     CDS             28..1389
                     /EC_number="6.3.1.-"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804484.1"
                     /locus_tag="YIM_RS30705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30935"
                     /product="glutamine synthetase family protein"
                     /protein_id="WP_153033652.1"
                     /transl_table=11
                     /translation="MDQHDREARAQQARGIAADLGERGIAAVVVTWVDTSGITRAKAVP
                     TARLGHAAAWGIGASPVFDAFLVDDSAVSGRFAGGPAGDLRLHPDLDRLTALAAQPGWA
                     WAPADRYQQDGTPHPADPRLLARQAVARLAEHGLTAQVAFEIEWCVSRGDGDEFVPASS
                     GPAYGMSRITDLPDYLRELLEALSAQGIAVEQLHPEYAVGQFEVSVAATDPVGAADLLV
                     LVRETVRAVGHRHGLRTTFAPKVVAGGVGNGGHVHLSLWRDGTNLMSDGDGWHGLTEQA
                     EAFAGGILDRLPGLLAIGAPSVASYLRLVPSHWAGPFRCWGWENREAALRLVSGVTGNG
                     QRAANLEIKAFDGAANPYLVVAALLVAGTTGIGEQAALPEPIQVDPASLPEGTVQRLPT
                     QLEAAVAAFEADPALRKALGEELVDTVVTVRRGEVALFDGRSAEEIVARTRWRY"
     gene            1407..1715
                     /locus_tag="YIM_RS30710"
                     /old_locus_tag="YIM_30940"
     CDS             1407..1715
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560769.1"
                     /locus_tag="YIM_RS30710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30940"
                     /product="hypothetical protein"
                     /protein_id="WP_153033653.1"
                     /transl_table=11
                     /translation="MAAAKNRSGDESNRRFHEDLVGLDPADPEAQEFARHLDRIERCGP
                     AFTVEASIDGVADFADSSRRAGGLRWWTAVAVVCLILIGVIVASWDIVLRTLAWLAE"
     gene            1735..2157
                     /gene="msrB"
                     /locus_tag="YIM_RS30715"
                     /old_locus_tag="YIM_30945"
     CDS             1735..2157
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671916.1"
                     /locus_tag="YIM_RS30715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30945"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_153033654.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLGPEEYQVLREAGTERPFTGEYTD
                     TKTTGVYECRACGAELFRSDTKFESHCGWPSFYDPADSDAVLLREDRTMGMVRIEVLCA
                     SCHSHLGHVFEGEGYDTPTDQRYCINSISLKLVPKP"
     gene            2228..3115
                     /locus_tag="YIM_RS30720"
                     /old_locus_tag="YIM_30950"
     CDS             2228..3115
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="YIM_RS30720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30950"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_153033655.1"
                     /transl_table=11
                     /translation="MGDPWGLSGPAFLGIYAGCLAVPTLVLIAGLLRLRSSGAEAGQVD
                     VHQAAFLRGGPQRAAETVLAGLVERGLLRVDSSGTLHATGRAGTTQLERHALASATGRS
                     SRAVINYLVGSGPMRALRADLVTRGLLIDSRRRRAVCLTAIVLYCAVLLLGLLRFADGA
                     EAGFPVGYLALMLVAVAGGLIISSVLRGRSDDKLTREGVRARAEGFPGAAGVVAQHSLA
                     SYPDREIAAALRHGMTPPRPRPRRSNSSCGSYAGGAIVGGGCSSSGGSSCSSSSSGGSS
                     CGGGGGCGGGGGGG"
     gene            3117..4034
                     /locus_tag="YIM_RS30725"
                     /old_locus_tag="YIM_30955"
     CDS             3117..4034
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="YIM_RS30725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30955"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_153033656.1"
                     /transl_table=11
                     /translation="MTETWGLSGSAFLGLYLALLVLPGLLGVLATLAVRQVRRHGSEPD
                     DSPLPSDYHVAYLARGPLRAVETAMAALLDRGSLRAESTGTLRSVGGEPEDDLERVIFE
                     TGYLGTTPNEAAVAALKSPELAEIRRELELRRLIEPELPTHVIWLGVTVLYGLITLFGL
                     VRWFAVVTTSTPIGFLSFLIFVAAGGTFAALAPARRRPRLRKTELGKQVLRSSRQTLTG
                     VTREVALNGGLDAYPDPEVARAVALKFNRSRRRYPATSSAGVIAGTLLAGVLHGAPGDS
                     GDSGGCGSGCGSGCGGGGGGGGGG"
     gene            4112..5008
                     /locus_tag="YIM_RS30730"
                     /old_locus_tag="YIM_30960"
     CDS             4112..5008
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="YIM_RS30730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30960"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_153033657.1"
                     /transl_table=11
                     /translation="MDEPWGISGPDFLVLYLFLLALPPAVAVVGRIALASGGSRAATAY
                     TPGNVYEVAFLAGGADRVVDTAVASLLEREVVRADSTGRVHGAGSPPADPVEQAVYEVI
                     VRRGSSRMADLRRELRKSAIIRGMTKHLTDAGLVQPTRGRVILRRLVHVLYLLTLALGV
                     IRLVNGIVRDRPVGFLIVLVVAAALATFIASRVARSAPPVRPTARGSVQLASAKNWRSR
                     QTASPGGAPSRSSAAVPLLAGAAGAVALGGMMMYPDEDLRTALAAPSSGGSSGGDSGGG
                     GSCGGGGGCGGCGGCGG"
     gene            5001..5810
                     /locus_tag="YIM_RS30735"
                     /old_locus_tag="YIM_30965"
     CDS             5001..5810
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157007.1"
                     /locus_tag="YIM_RS30735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30965"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_370468898.1"
                     /sec_met_domain="DUF692 (E-value: 3.1e-83, bitscore: 278.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELPRFGVGIGWRPELDLSVPRLPGVDWVEVVAENLQPGALPAS
                     LTALRDRGVPVLPHAVSLSLGGAEELDTRRVEHLAELTAALGAPVASDHVCFVRAGGLD
                     SGHLMPLPRTREALDVLVDNVKLAQSILGVPFALENIAALLEWPDPELSEARFLTELTD
                     RTGCLLIVDVANLYANARNLGSDPVSFLDEIPLDRLAYVHVAGGEEHHGVYHDTHAHAV
                     PPQVLDLLGELRSRVDPPGVLLERDDAYPTDAELAAELAAIRAVVDA"
     gene            5807..6154
                     /locus_tag="YIM_RS30740"
                     /old_locus_tag="YIM_30970"
     CDS             5807..6154
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684641.1"
                     /locus_tag="YIM_RS30740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30970"
                     /product="hypothetical protein"
                     /protein_id="WP_153033658.1"
                     /transl_table=11
                     /translation="MSRERLAAQQAELLRALLAGGAAPAGFDPGRVEVEVRALRAKRRR
                     IVAYLRPELPQELGDRFVPLFTEYAATHPREEGVRMRQDAENFANWLTARGELKRPRWW
                     QRLPGRAVRAR"
     gene            complement(6125..7459)
                     /locus_tag="YIM_RS30745"
                     /old_locus_tag="YIM_30975"
     CDS             complement(6125..7459)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224515.1"
                     /locus_tag="YIM_RS30745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30975"
                     /product="CoA transferase"
                     /protein_id="WP_228005040.1"
                     /transl_table=11
                     /translation="MEEELAAVWRQVSGESLTPGAVTVSGDESVLPGPFRVAAAAAASV
                     GVATLAAAELLRLRGIEPPPVEVEIRHAVAAFHSERHLRVDGEVPGELWAPLTGDYLAT
                     DGWVRLHCNYPRHVSAVCWGLGAAATREAVTEAVAKRRAREVQEAVIAAGGAAALMRTR
                     QDWLNHPQGAAVAGQPLVELHQTGSAPPKRLFESDRPLGGVRVLELTHVIAGPVAGRVL
                     AAHGADVLHIGAAHLPRIDPLWIDTGLGKRSAFTDLTQDAGRARLRKLLAKADVFVQSF
                     RPGALARWGFSPEELADEFPGLITLDLSAYGPSGPWRNRRGFDSLVQLATGIAAHAGLD
                     RPHPLPAQALDHATGWLAAATVMTALRRAVTDGGGWRGQVALARTAEWLESLGRREITD
                     TGYTADDLLAKLPSPYGELTCVPVPGANPQWTEGTRLPGTDRPSW"
     misc_feature    complement(7310..7312)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7469..7798)
                     /locus_tag="YIM_RS30750"
                     /old_locus_tag="YIM_30980"
     CDS             complement(7469..7798)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="YIM_RS30750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="YIM_30980"
                     /product="hypothetical protein"
                     /protein_id="WP_153033659.1"
                     /transl_table=11
                     /translation="MSFPEIDRKQKQMRHDIDDIHELLDHTNKRVDVIDTKVESILEVQ
                     EAHGEQLHKIDERLHGIETRLSGHDAQFETLNARLDGQSARLDSQGEQLNQIIELLRGQ
                     RPETN"
     gene            complement(7849..8541)
                     /gene="hemQ"
                     /locus_tag="YIM_RS30755"
                     /old_locus_tag="YIM_30985"
     CDS             complement(7849..8541)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="YIM_RS30755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30985"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_153033660.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEPGRLPEDRGPAARETTEFLDG
                     LESKGVVVRGVYDVAGLRADADYLIWWHAEEIEQVQAAYTGFRRTPLGRASVPVWSQVA
                     LHRPAEFNRSHIPAFLAGEEPRKYVCVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTRVPP
                     AELIQNLP"
     gene            complement(8529..9947)
                     /gene="hemG"
                     /locus_tag="YIM_RS30760"
                     /old_locus_tag="YIM_30990"
     CDS             complement(8529..9947)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675019.1"
                     /locus_tag="YIM_RS30760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_30990"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_153033661.1"
                     /transl_table=11
                     /translation="MTTRIAVVGGGVSGLAAAYRLRRLLGADAELTVFESTGAPGGKLR
                     TGTLAGRPFDLGAEAFLARRPEAVALARELGLEVVHPTGARATVRAGGRARRLPPGTMM
                     GVPASADAVSSVLSPEGLAAVAAEAELTPVELPAEDVPLGGLLRSRFGDELVDRLVDPL
                     LGGVYAGGADGLGLRATMPGLAGALDRGVPTLTEAAASLVPASPSSAPVFGTLGSGLGT
                     LIERLVAASGARVRTGTTVRELHRLSGGWRLVLGAAATAHAPEESTVDFDAVLLAVPAP
                     AARRLLDGVAPAASAAYGRVELASMAVIGLALPPGTELPDASGVLVGASERREDGRPFA
                     VKAFTFSARKWAHHGGDEVLLRGSVGRFREPGALHADDDELVRLVLDDLAELTGVRADP
                     VDSVVTRWGGGLPQYGAGHTGLVASIDRAVAELPGLEVAGATLRGVGIPACVATADAAA
                     TRLAEFAKMDAWRG"
     misc_feature    10612..10626
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
ORIGIN
        1 cagaccgcgg gtgaaggggt ttgagggatg gaccagcacg atcgcgaggc acgcgcccag
       61 caggcccgcg ggatcgccgc ggacctcggc gagcgcggga tcgccgccgt cgtggtgacc
      121 tgggtggaca ccagcggcat caccagggcc aaggcggtgc ccacggcccg gctcgggcac
      181 gccgccgcct ggggcatcgg cgcctctccc gtgttcgacg ccttcctggt cgacgactcc
      241 gcggtgagcg gccggttcgc cggcggcccg gccggtgatc tgcggctgca ccccgatctg
      301 gaccggctca ccgcgctggc cgcgcagccc ggctgggcgt gggcgcccgc cgaccgctac
      361 cagcaggacg gcacgcccca tcccgccgat ccgcgcctgc tcgcccgcca ggccgtcgcc
      421 aggctcgccg agcacgggct gaccgcgcaa gtggccttcg agatcgagtg gtgcgtctcc
      481 cgcggtgacg gcgacgagtt cgtcccggcc agctcgggcc cggcctacgg catgtccagg
      541 atcaccgatc tgccggacta cctgcgtgaa ctgctggagg cgctgagcgc gcagggcatc
      601 gcggtcgagc agctgcaccc ggagtacgcc gtcggccagt tcgaggtgtc ggtggcggcc
      661 accgacccgg tcggcgcggc ggacctgctg gtgctggtgc gggagaccgt tcgcgcggtc
      721 ggccaccgcc acggcctgcg caccaccttc gcgcccaagg tggtggccgg cggagtcggc
      781 aacggcgggc acgtgcacct gagcctgtgg cgcgacggca cgaacctgat gtccgacggc
      841 gacggctggc acgggctcac cgagcaggcc gaggccttcg ccggcggcat cctggaccgg
      901 ctgcccggcc tgctcgccat cggtgcgccc tcggtggcca gctacctgcg gctggtgccg
      961 tcgcactggg ccggtccgtt ccgctgctgg ggctgggaga accgcgaagc cgcgctgcgg
     1021 ctggtctccg gtgtgaccgg gaacggccag cgcgcggcga acctggagat caaggcgttc
     1081 gacggggccg cgaacccgta cctcgtggtg gccgcgctgc tggtcgcggg caccaccggg
     1141 atcggcgagc aggcggccct gcccgagccg atccaggtcg atccggcctc cctgcccgag
     1201 ggcaccgtgc agcggctgcc cacccagctc gaagccgcgg tcgccgcgtt cgaggcggac
     1261 cccgcgctgc gcaaggcgct cggtgaggag ctggtggaca ccgtggtcac cgtgcgccgt
     1321 ggtgaggtgg cgttgttcga cgggcggtcc gccgaagaaa tcgtggcgcg cacccgatgg
     1381 cggtactgac gcgataccgt gaggccatgg ccgctgccaa aaacaggtcc ggcgacgagt
     1441 cgaaccgccg gttccacgag gacctcgtcg ggctggaccc ggcggacccc gaagcgcagg
     1501 agttcgcccg ccacctcgac cggatcgagc gctgcggtcc cgcgttcacc gtggaagcct
     1561 cgatcgacgg agtggccgac ttcgccgact ccagccgccg cgcgggcggt ttgcgctggt
     1621 ggaccgcggt ggccgtggtg tgcctgatcc tgatcggcgt gatcgtcgcc tcgtgggaca
     1681 tcgtgctgcg gacgctggcc tggctcgccg agtaacgtgg agaacgtgaa aggtatgaaa
     1741 cccgtcgtcg gcgcgactcc ccgcgtggtg aagtccgagc aggagtggcg ggagcagctc
     1801 ggccccgagg agtaccaggt gctccgggag gccggcaccg aacgcccgtt caccggcgag
     1861 tacaccgaca ccaagacaac cggcgtctac gagtgccgtg cctgcggggc cgagttgttc
     1921 cgcagcgaca cgaagttcga aagccactgc ggctggccgt cgttctacga cccggccgac
     1981 tccgacgcgg tactgctccg cgaggaccgg accatgggca tggtgcgcat cgaggtgctg
     2041 tgcgcctcgt gccacagcca cctggggcac gtgttcgagg gcgagggcta cgacacgccg
     2101 accgaccagc ggtactgcat caattcgatc tcgctgaaac tggtgcccaa gccgtaaccc
     2161 gttccaggga caagcgcctc ccccacccga taccttggcg atcacgcttc gggtggggag
     2221 gcaacgaatg ggtgatccgt ggggcttgtc cggcccggct tttctcggca tctacgccgg
     2281 gtgcctggcc gtgcccacgc tggtactgat cgccgggctc ctgcggctcc gctcgagcgg
     2341 tgccgaggcc gggcaggtcg acgtgcacca ggcagccttc ctccgcggcg ggccgcaacg
     2401 tgccgccgag accgtgctgg ccgggctggt cgaacgcggg ctgctgcggg tcgattcctc
     2461 gggcaccctg cacgccactg gccgcgcggg gacgacccag ctggaacggc acgcgctcgc
     2521 gagcgcgacg ggccggtcct ctcgagcggt gatcaactac ctcgtcggct ccggcccgat
     2581 gcgggcgctg cgggcggatc tggtcacccg gggcctgctg atcgacagcc ggcgacgccg
     2641 cgcggtgtgc ctcaccgcga tcgtcctgta ctgcgccgtg ctgctgctcg gcctgctccg
     2701 gttcgccgac ggcgcggagg ccggcttccc ggtcggctac ctggcgctga tgctggtggc
     2761 ggtcgccggt gggctgatca tctcgtcggt gctgcgcgga cgcagtgatg acaagctcac
     2821 ccgggagggc gtacgggctc gtgccgaggg ctttcccggt gcggcgggcg tggtggccca
     2881 gcacagcctc gcgtcgtacc cggaccgcga aatcgccgcg gcgttgcggc acggcatgac
     2941 cccgccccgt ccccggccgc ggcggtcgaa ttcttcgtgc ggaagctacg ccggtggtgc
     3001 catcgtgggt ggtggttgct cgtcgtcggg cggaagcagc tgttcgtcgt cctcctcggg
     3061 tggcagcagc tgcgggggcg gcggcgggtg cggtggtggt ggcggaggag gctgagatga
     3121 ctgagacgtg gggcctgtcc ggatccgcgt tcctcgggct gtacctggcg ttgctggtgc
     3181 tgccgggact gctgggcgtg ctggccacgc tggcggtccg gcaggtgcgg cggcacgggt
     3241 ccgaaccgga cgactcgccg ctgcccagcg actaccacgt cgcctacctg gcccgcggac
     3301 cgctgcgtgc ggtcgagaca gccatggccg ccctgctcga ccggggcagc ctgcgtgccg
     3361 agagcaccgg gacgctcagg tcggtcggcg gcgagccgga ggacgatctc gagcgcgtca
     3421 tcttcgagac cggctatctc ggcaccaccc cgaacgaagc cgcggtcgcc gcgctgaagt
     3481 cgccggagct ggccgagatc cgccgtgaac tcgaactccg caggctgatc gagccggagc
     3541 tgcccaccca cgtgatctgg ctcggcgtga ccgtgctcta cggcctgatc acgttgttcg
     3601 gcctggtgcg ctggttcgcc gtggtcacca cgagcacgcc gatcggcttc ctcagcttcc
     3661 tgatcttcgt cgcggccggt ggcaccttcg ccgcactggc gcccgcccgg cggcggccgc
     3721 gactgcgcaa gaccgaactg ggcaagcagg tgctgcgctc gtcgcggcag acgctgaccg
     3781 gcgttacgcg cgaggtcgcg ctgaacggcg gactcgacgc gtatcccgat cccgaagtcg
     3841 cgcgggcggt cgcgctgaag ttcaaccgct cccgccgccg ctacccggcc accagcagcg
     3901 ccggggtgat cgccggaacc ctgctcgccg gggtgctcca cggggcgccg ggcgacagcg
     3961 gcgacagcgg tggctgcggt tcgggctgcg gcagcgggtg cggtggtggc ggcggtggcg
     4021 gaggcggcgg ctgacggcgg aaatacggcc ctgcgcagcc ggtgcggtgg cttactctcc
     4081 gagcagcttc gatcggttgg agggcggagt gatggacgaa ccctggggca tctctggccc
     4141 ggatttcctg gtgctgtacc tgttcctgct ggccttgccg ccggcggtcg ccgtggtggg
     4201 ccgcatcgcg ctcgcctccg gcgggagccg cgcggccacc gcgtacaccc ccggcaacgt
     4261 ctacgaggtg gcgttcctgg ccggcggtgc cgaccgggtc gtcgacaccg cggtggcttc
     4321 gctgctcgaa cgcgaggtgg tccgcgcgga cagcaccggc cgggtgcacg gagcgggcag
     4381 cccgcccgcc gatccggtgg agcaggccgt gtacgaggtg atcgtgcgcc ggggcagctc
     4441 ccggatggcc gacctgcgcc gggaactgcg caagtcggcg atcatccgcg ggatgaccaa
     4501 gcacctgacc gacgcgggcc tggtccagcc gacgcgcggg cgcgtcatcc tgcgccgcct
     4561 ggtgcacgtg ctctacctgc tcaccctggc gctcggggtg atccgcctgg tcaacggcat
     4621 cgtgcgggac cggccggtcg gcttcctgat cgtgctggtg gtcgcggccg cgctggccac
     4681 gttcatcgcg tccagggtgg cccggtcggc gccaccggtc cggccgaccg cgcggggctc
     4741 ggtgcaactc gccagcgcca agaactggcg atcccgccag accgcgtcgc cgggcggtgc
     4801 cccgtcccgg tcttcggccg cggtgccgct gctagccggt gccgcgggcg cggtcgcgct
     4861 gggcggcatg atgatgtacc ccgacgagga cctgcgcacc gcgctggccg ccccgagttc
     4921 gggtggttca tccggtgggg acagcggtgg cggcgggtct tgtggcggag gcggcgggtg
     4981 cggcggctgc gggggttgcg gtggctgagc tgccccggtt cggcgtcggg atcggctggc
     5041 ggcccgagtt ggatctctcg gtgccgcgcc tgcccggtgt cgactgggtg gaggtggtcg
     5101 ccgagaacct ccagcccggc gcgctgcccg cgtcgctgac cgcgttgcgc gatcgcggcg
     5161 tgccggtgct gccgcacgcg gtgtcgcttt cccttggcgg ggccgaagaa ctggacaccc
     5221 ggcgcgtcga gcacctggcc gagctgaccg cggcgctcgg tgcgccggtg gccagcgacc
     5281 acgtgtgctt cgtacgcgcg ggcgggctgg actcgggtca cctgatgccg ctcccccgca
     5341 cgcgcgaggc gctggatgtg ctggtggaca acgtgaagct ggcgcagtcc attctcggtg
     5401 tgccgttcgc gctggagaac atcgcggcgc tgctggagtg gcccgatccg gagctgagcg
     5461 aagcgcggtt cctgaccgag ctgaccgacc gcaccggctg cctgctcatc gtggacgtgg
     5521 cgaacctgta cgcgaacgcg cggaacctgg gcagcgatcc ggtgtccttc ctggacgaga
     5581 tcccgctgga ccggctggcc tacgtgcacg tggccggcgg cgaggaacac cacggcgtct
     5641 accacgacac gcacgcccac gcggtgccac cgcaggtgct cgacctgctc ggcgagctgc
     5701 ggtccagagt ggacccgccg ggcgtgctgc tggaacgcga cgacgcctac cccaccgatg
     5761 ccgaactggc cgccgagctg gccgcgatcc gcgcggtggt cgacgcgtga gccgggagcg
     5821 actggcggca cagcaggcgg aactgctgcg ggcgctgctc gccgggggcg ccgccccggc
     5881 cgggttcgat cccggccggg tggaggtcga ggtgcgggcg ttgcgggcga agcggcgccg
     5941 gatcgtcgcg tacctgcggc cggagttgcc gcaggagctg ggcgatcgct ttgtcccgct
     6001 gttcaccgaa tacgcggcca cgcaccctcg tgaggaaggg gtgcggatgc ggcaggacgc
     6061 ggagaacttc gcgaactggc tcaccgccag gggcgagctg aagcggccgc gctggtggca
     6121 gcggctacca ggacgggcgg tccgtgcccg gtagccgggt tccctccgtc cactgtggat
     6181 tcgcgccggg taccgggacg caggtgagtt cgccgtacgg gctgggcagc ttcgccagca
     6241 ggtcgtcggc ggtgtaaccg gtgtcggtga tttcgcggcg ccccaaggat tccagccatt
     6301 ccgcggtgcg cgcgagcgcg acctgaccgc gccagccacc gccgtcggtg accgcgcggc
     6361 ggagcgcggt catcaccgtc gcggccgcga gccagccggt ggcgtggtcg agtgcctgcg
     6421 cgggcagcgg atggggccgg tccagcccgg cgtgcgcggc gatgccggtg gccagctgca
     6481 ccaggctgtc gaatccgcgc cggttgcgcc acggaccgct cgggccgtag gcggacaggt
     6541 cgagggtgat caggccgggg aactcgtcgg ccagttcctc gggcgagaag ccccagcggg
     6601 ccagcgcgcc gggccggaag gactggacga acacgtccgc cttggccagc agcttgcgca
     6661 gccgggcccg gccggcgtcc tgggtgagat cggtgaaggc ggaccgcttg cccagcccgg
     6721 tgtcgatcca cagtggatcg atgcggggca ggtgcgcggc gccgatgtgc agcacgtcgg
     6781 ccccgtgcgc ggcgagcacc cggccggcca ccggcccggc gatgacgtgg gtcagttcga
     6841 gcacacggac cccgccgagc gggcggtcgg actcgaacag ccgcttcggc ggcgcgctcc
     6901 cggtctggtg cagctcgacc agcggctgcc cggcgacggc tgcgccctgc gggtggttga
     6961 gccaatcctg gcgggtgcgc atcagcgcgg cggccccgcc ggcggcgatc accgcctcct
     7021 ggacctcgcg cgcccggcgc ttggcgacgg cctcggtgac ggcttcccgg gtggccgcgg
     7081 cgccgagccc ccagcagacg gccgaaacgt ggcgcgggta gttgcagtgc agccggaccc
     7141 acccgtcggt ggcgaggtag tccccggtca gcggcgccca gagttccccg ggcacttcgc
     7201 cgtccacccg gaggtggcgc tcactgtgga acgcggcgac cgcatggcgg atctccacct
     7261 ccaccggcgg cggttcgatc ccccgcaggc gaagaagctc agcggcggct aacgtcgcca
     7321 cccccacact cgccgccgcg gccgccgcca cccggaacgg cccgggcagc acggactcgt
     7381 ccccgctgac cgtcaccgcc cccggcgtca ggctttctcc gctgacctgc cgccacaccg
     7441 cggcgagttc ttcctcgatc acgttccctc agttggtttc gggccgctga ccgcgcaaca
     7501 gctcgatgat ctggttcaac tgctccccct ggctgtcgag cctcgccgac tgcccgtcca
     7561 ggcgagcgtt gagggtctcg aactgggcgt cgtgtccgct caggcgggtc tcgattccgt
     7621 gcagcctctc gtcgatcttg tgcagctgct cgccgtgagc ctcctgcacc tccaggatgg
     7681 actccacctt cgtgtcgatg acatcgactc tcttgttcgt gtggtccagg agttcgtgga
     7741 tgtcgtcgat gtcgtggcgc atctgcttct gcttgcggtc gatctcggga aaagacatgc
     7801 gggacaccct accctctcca cgaacaagtg gcctatgcca cccctgatct acggcaggtt
     7861 ctggatcagc tccgccggtg gcacccgcgt gccggtgtag aacgggatct cctcccgcac
     7921 gtgcagccgc gcttccgtgc cgcgcaggtg acgcatcagg tccacgatcc ggtgcagctc
     7981 gtccgcttcg aaggcgagga tccattcgta gtcacccagc gcgaacgaag cgacggtgtt
     8041 cgcgcgcacg tccgggtagt cgcgcgcttc cttgccgtgg tcggcgagca tcttgcggcg
     8101 ctcgtcgtcc ggcagcaggt accactcgta ggaccgcacg aacgggtaca cgcagacgta
     8161 cttccgcggc tcctcccccg ccaggaaggc cgggatgtgg ctgcggttga actccgcggg
     8221 ccggtgcagc gcgacctggc tccacaccgg caccgacgcc cggcccagcg gcgtccgccg
     8281 gaacccggtg taggcggcct ggacctgctc gatctcctcg gcgtgccacc agatcaggta
     8341 gtcggcgtcg gcccgcagac cggcgacgtc gtacaccccg cgcaccacca cgcccttgct
     8401 ctcgagaccg tcgaggaact cggtggtctc ccgcgcggcg gggccgcggt cctcgggcaa
     8461 ccggcccggt tcggcccgga acaccgacca ggcggtgtag cgaatggtgt cgttgagctc
     8521 gttgtagttc agccgcgcca tgcgtccatc ttcgcaaact ccgcgagcct ggtggcggcg
     8581 gcgtccgcgg tggcgacgca ggcgggaatg ccgacgccgc gcagcgtggc gcccgccact
     8641 tccaggcccg gcagctcggc cacggcccgg tcgatcgacg cgaccagccc ggtgtgcccc
     8701 gcgccgtact gcggcagtcc gccaccccag cgcgtgacca cggagtccac cggatcggcc
     8761 cgcacgccgg tcagctcggc gaggtcgtcg agcaccagcc gcacgagttc gtcgtcgtcg
     8821 gcgtgcagtg cgcccggctc gcggaaccgg ccgaccgacc cgcgcagcag cacctcgtca
     8881 ccgccgtggt gcgcccactt cctggccgag aaggtgaacg ccttgaccgc gaacggcctg
     8941 ccgtcctcgc gccgctccga cgccccgacc agcacgccgg acgcgtccgg cagctcggtc
     9001 cccggcggca gggccagccc gatcaccgcc atcgacgcca gttccacccg tccgtacgcg
     9061 gccgaggccg ccggggcgac cccgtcgagc agccgcctcg ccgccggggc gggtaccgcc
     9121 agcagtaccg cgtcgaagtc cactgtggac tcctcgggcg cgtgcgcggt ggccgcggcg
     9181 ccgagcacca ggcgccagcc gccggacagc cggtgcagct cacgaacggt cgtcccggtc
     9241 cgcacccgcg ccccggacgc cgccaccagc cgctcgatca acgtgccgag ccccgagccg
     9301 agcgtgccga acaccggtgc gctgctcggc gacgcgggca ccagcgacgc ggccgcctcg
     9361 gtcagcgtcg gcacgccacg gtcgagcgca ccggccaacc ccggcatggt ggcccgcagc
     9421 ccgagcccgt ccgctccccc ggcgtagacc ccgccgagca gcggatcgac caggcggtcc
     9481 accagttcgt ccccgaagcg cgaccgcagc agcccgccca gcggcacgtc ttccgcgggc
     9541 aactcgaccg gcgtcaactc ggcttcggca gccaccgcgg ccagtccctc cggggaaagc
     9601 acgctggaaa ccgcgtcggc gctggcgggt acccccatca tcgtgccggg cggcaaccgg
     9661 cgtgcccggc caccggcacg cacggtcgcc cgcgccccgg tcgggtgcac cacctcaagc
     9721 cccagctcgc gtgccagcgc caccgcttcg ggacgacgcg ccaggaaggc ttcggcaccg
     9781 aggtcgaacg gtcgtcccgc cagggtgccg gtgcgcagct tgccgccggg cgccccggtc
     9841 gactcgaaca cggtcagctc ggcgtccgca ccgagcaggc ggcgcagccg gtaggccgcg
     9901 gccagcccgg agacaccgcc gccgaccacc gcgatcctgg tggtcatgat cagagcgagt
     9961 gcacgagttc gacgacccgg gtgatcacct cggggtcggt gtcgggcagc acgccgtggc
    10021 ccaggttgaa gatgtgcccg gcggccgcgc ggccctcttc ggcgatccgg cgcacctcgg
    10081 cctcgagcac cggccacgag ccgaacagca gcgccgggtc caggttgccc tgcaccaccg
    10141 gcgccgaacc gggccgggtg gcggtcagcc tgcggacggc ctcgtcgagc ggcacgcgcc
    10201 agtcgacgcc caccacgtcg ggaccggcgt cacgcagatc ggacagcagt tccccggtgc
    10261 cgaccccgaa gtggatgcgc ggcacgcccg cgtcggccac cccggcgaac accttcgccg
    10321 aatgcggcag cacgaactcg cggtagtcgc gcggcgagag cgcgcccgcc cacgagtcga
    10381 acagctggat cgcgtcgacc ccggcggcga tctgcacccg caggaagccg agcgcgatgt
    10441 cggcgagctt gccggccagc gcgtgccaga ccgcgggctc ggagtgcatc agcgccttgg
    10501 tgcgctcgtg gttgcggctc gggccgccct cgatcaggta ggaggccagc gtgaacggcg
    10561 cgccggcgaa cccgatcagc ggggtgtcgc cgagccgctc gaccagcagg cggatgccct
    10621 cggccaccgg gacgacctgc tcggcgtcca gttcgggcag cgcggccacc gcggcggcgt
    10681 cccgcaccgg gccggccacc accgggccgg tgccggccac gatgtcgatg tccaggcccg
    10741 ccgccttgag cggcaccacg atgtcgctga acaggatcgc cgcgtcgacc ccgtgccgcc
    10801 gaaccggctg
//
