LOCUS       NZ_CP045480            12851 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Amycolatopsis sp. YIM 10 chromosome, complete genome.
ACCESSION   NZ_CP045480
VERSION     NZ_CP045480.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. YIM 10
  ORGANISM  Amycolatopsis sp. YIM 10
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Complete genome sequence of Amycolatopsis sp. YIM 10, isolated from
            a rare earth mine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2019) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009429145.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 04:03:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,775
            CDSs (total)                      :: 9,711
            Genes (coding)                    :: 9,634
            CDSs (with protein)               :: 9,634
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 22 of 77
            Pseudo Genes (incomplete)         :: 65 of 77
            Pseudo Genes (internal stop)      :: 1 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:01:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4268468
            Orig. end                         :: 4281319
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045480.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12851
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="13"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12851
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12851
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7851](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7851
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(79..90)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(301..317)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.36"
                     /tool="antismash"
     misc_feature    426..441
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.92"
                     /tool="antismash"
     misc_feature    complement(648..662)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     gene            887..3523
                     /locus_tag="YIM_RS20585"
                     /old_locus_tag="YIM_20730"
     CDS             887..3523
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /locus_tag="YIM_RS20585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20730"
                     /product="P-loop NTPase fold protein"
                     /protein_id="WP_153031901.1"
                     /transl_table=11
                     /translation="MDTAPDAPPILPEAAGPPAEALPPKRGSAGAGLTIALVSGPVYRH
                     PWFEDAVDAAVDDALTSWGTMAAGLVLQRAPAARVLNCSMSDQDTITDGQMLASLAKAR
                     AANADLIVIGFGGEPADAPSLTFTKVVGTIEGLVLAAAGSNGTADRPDHPANVPGVVAV
                     GGLVHDREEGWKRSPFSSPGEIYAPCVDLLTTTVGENGPGFARLGGTMMATALVAGEIA
                     ATMTRLRVSAGEALERLKRLPPEVFLEAHEGNAAGPPPISAEPVALAGPTAGVNDYWTT
                     TDRLGYQLHADALASLILDEGTKPPLTVAVKGEWGTGKTSLLRMTQARIDPLLDSGERR
                     AIRLTRTSHGKLDRVSSGAPRLATVFKLLRRAEVREVVRDKPSSFGTRLRTTELRAEST
                     GRWRPTVWFNPWMYQTGEQVWAGLAHEIINQVTQRLPLIDRERFWLALNLSRLDRVAVR
                     GRVLRTVLARLLPALATSVLALVAGGVLLASGNAGWGAGVASAGVLGVVFRTVRLLWSR
                     TDAVLPGLVTPPGFAGVVTGIAGGIADSVDDPRYRSRAGFLHMVHNDVAAVLDLVATRD
                     RPLVVFVDDLDRCTSGAVIQLVEALNLFLAGQFPNCVFVVAMEPEVVAARLDAAYEPSG
                     SGHSTGWRFLDKLVQLEFSLPAPDAAAMQGYISTTLTGSEAGEPTGPGSVSEEAVRAAQ
                     RVLGSMGSVHDVADRARAIEGPGTDERSPVLWEAAKRETLRRLTGDDPEVRAILATISA
                     HLGRNPREIKRVINLIRFHSVINLGAAQRGGHTPLSIYQLTKLALIAVRWPHLRSVLAG
                     QAGRPGTIVLTAAEDTLRGAQGGSIRDRLVGQGVDAASAAVLGANADLLRLLADNPPIG
                     PGAVRHL"
     gene            complement(3534..4145)
                     /locus_tag="YIM_RS20590"
                     /old_locus_tag="YIM_20735"
     CDS             complement(3534..4145)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223660.1"
                     /locus_tag="YIM_RS20590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20735"
                     /product="DUF6801 domain-containing protein"
                     /protein_id="WP_153031902.1"
                     /transl_table=11
                     /translation="MFSRIATTAAVFAVAVLGTAGTAFAATITYRTGTAAPVVYRCSLP
                     SLPPQPGTVEARFDAPDSVPSGTTFTPTGVGGSITFTPETHEQFAAAGWDGIRGSAHLY
                     LTTTGATLSPPAANNLTVPETLFPAGGPMVIPFGQSATSTVPAVTAGAPGTATVSSSTV
                     LVAGEFHRPTTGQWHAVTMSCYLHPTVPQNPVFSPAITVS"
     misc_feature    complement(4323..4336)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.16"
                     /tool="antismash"
     gene            4357..4932
                     /locus_tag="YIM_RS20595"
                     /old_locus_tag="YIM_20740"
     CDS             4357..4932
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945565.1"
                     /locus_tag="YIM_RS20595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20740"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_153031903.1"
                     /transl_table=11
                     /translation="METSDPSVRDLLAANIKRARADRGLSLSELSRRSSIGKATLSQLE
                     SGAGNPTIETVFSLSRALELPISDLLESGHHGDMTVVRGDEQEVLAGEAVELRMLRRIE
                     ADGLIFEVYDQRVRGSAGQRSLGHVGTEHTIVQAGRLRVEVGGSTAELGPSDSVAFDAS
                     LPHSYQAIGGEVRSVLLLQYRGGSSPHA"
     gene            5001..6152
                     /locus_tag="YIM_RS20600"
                     /old_locus_tag="YIM_20745"
     CDS             5001..6152
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225094.1"
                     /locus_tag="YIM_RS20600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20745"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_153031904.1"
                     /sec_met_domain="DAO (E-value: 1.7e-61, bitscore: 207.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGTRVVVIGAGIVGAACARELCAEGFQVTVVDRGRPGGATTAHG
                     EGNVLVSDKGPGAELELARLSRRLWPEVLAELPAAEGVEWDAKGGIVVATTESGARALT
                     EFAEGQRAAGIVAEPMDAAALGDAEPHLTRDVTAAIHYPEDAQVQPVAAATALLAAAGV
                     TLYTRCEVTGALTDSGRLTGIRTTGGDLPADLVVNAAGPWAGELSAVLGVPIDVRPRRG
                     EVLVTTPMPPTVFHKVYDADYVGAVGSGSAELQTSAVVESTKAGTILLGSSRRQAGFDD
                     RLHLGALSAIAAKALRLFPGLAGVSVMRAYGGFRPYVPDHLPVLGADPRLPGLWHASGH
                     EGAGIGLSVGTGRVLRDLITGRRPEIDAGPFRVDRPAVLGGAR"
     gene            6149..6457
                     /locus_tag="YIM_RS20605"
                     /old_locus_tag="YIM_20750"
     CDS             6149..6457
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029078.1"
                     /locus_tag="YIM_RS20605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20750"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_153031905.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.5e-24, bitscore: 83.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPRLVPGGTDRVRRRDRPIRLTVDGEPVSGVDGQTIAGVLLAAG
                     RTAWRTDPAGEPRGVFCGIGACFDCVVTVGAERDVRACRRRARDGDEVRTQARAEDR"
     gene            6454..7851
                     /locus_tag="YIM_RS20610"
                     /old_locus_tag="YIM_20755"
     CDS             6454..7851
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029077.1"
                     /locus_tag="YIM_RS20610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20755"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_153031906.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.6e-24, bitscore:
                     84.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.9e-08, bitscore:
                     32.1, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRVVIVGAGPAGLAAADAALRAGAGVSLLDAAEQPGGQYHRMLP
                     AAFRAELPDKIQHGRRAFDRRRRRVLDHPRCTWLPETSVWSLELRDGEPPLVHALRGVA
                     DGADRESVTLVPDALVLATGAHDRVLPFPGWTLPGVFTAGAAQALAKGERLAVGENVVV
                     SGTGPFLLPVAASLSQVDARVLAVFEANQPRTVLRGWTRDAGELVPHLKKAGELAGYAA
                     GQLRQRVPYRMGRAVVEARGDGRVEEVVVARLRADWSIIPGTGRTLAADAVCVGHGFTA
                     QLELPVAAGCDLAGDAVLVDAGQRTSRPGVFAAGEITGIAGAPSAVDSGTVAGWLAAGG
                     DPARITGALRRRDRGRAFGRRLARAHPIGGGWTGWLRPDTVICRCEAVDHACLNRALAA
                     PVTAGARSVKLATRAGLGPCQGRICGPAVAELSAAAGAEVARPHHRPIAQPIRLGELAN
                     LRTEENP"
     misc_feature    6607..6609
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7848..8765
                     /locus_tag="YIM_RS20615"
                     /old_locus_tag="YIM_20760"
     CDS             7848..8765
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153898.1"
                     /locus_tag="YIM_RS20615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20760"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_153031907.1"
                     /transl_table=11
                     /translation="MSTKADLGGVVVATALPYREDGQAPAGLAVDYDRYAEHCRWLIDN
                     GCRGIGPNGSLGEYSSLTDEERRRVARTAIEAVGDDGIVVVGVHGVGSHQAKHWAELAA
                     EDGAHGVLCLPPTMYRANRGEVLAHFEAVASAGLPVMVYNNPHDTKVDLTPDLLGEIAR
                     IENVVAVKEFSGDVRRVLEIKEHAPDLAVISGADDVVLEMLLMGSTGWFAGFPNVFPAE
                     SVELYDLATRGELARARELYEPLVAAFRWDSRTEFVQAIKLGMDLVGRYGGPCRPPRGP
                     LIPAHDEQVRADMARAIDALAGRS"
     gene            8765..9766
                     /locus_tag="YIM_RS20620"
                     /old_locus_tag="YIM_20765"
     CDS             8765..9766
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646747.1"
                     /locus_tag="YIM_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20765"
                     /product="proline racemase family protein"
                     /protein_id="WP_153031908.1"
                     /transl_table=11
                     /translation="MRSIRTLSAVDSHTEGMPTRVITGGVAPIPGDTIAARRRHFIDHL
                     DDLRRFLVDEPRGHPAMSGAILQPPTRPDADWGVLYVEVSGVLPMCGHGTIGVATVLVE
                     TGMVPVTEPETVVRLDTPAGLVQARVQVRDGVAERVTLRNVDAFAVELGATVDVPGLGE
                     VRYDMAYGGNFYAIVDLESIGLPFDRARKNDILDAGLRIMAAVNERNRPRHPVDEHIGG
                     CKHVQFLAPGSDAKHSRNAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLHTEFVNES
                     FIGTRFTGQLLGTTEVGGLPAVVPEFSGRAWITGTANYLLDPRDPFPTGFVL"
     gene            complement(9912..10502)
                     /locus_tag="YIM_RS20625"
                     /old_locus_tag="YIM_20770"
     CDS             complement(9912..10502)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /locus_tag="YIM_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_20770"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_153031909.1"
                     /transl_table=11
                     /translation="MSTLRPPRQDRAAATRARLIDAAYACLLEGGYSATTVGAVQQRAG
                     VARGTLLHHFPTRATLMAAVVDDIADRRVRVLSSGTAGPPADGGWDGVVDLVWRDLQSP
                     AFAAALELWVASRTDPELRAALAPVQQRVITAARHAIIAHVGDDDPRVPTLAQFTIDLL
                     TGTAMSRVVDPDADVTVVVQRWKQTLATLSGGR"
ORIGIN
        1 gggccgagcg ggtggcgatg gtggcgcggt cctcgtcggt gtaggggtag gtggccgctt
       61 cctcggggct cgggagctga atgcgttttc cgcgcaatcg gctgagcacc ttcaaccgcg
      121 tcgggccggc gagccactcg gcgcggtcgt cggtttcggc ggcgatcacc gaaacgctga
      181 ccaaggtcct cggagtggag gcgtattccg atgggcggaa gctgtcgcgg taggcgcgca
      241 tcgcccctcc ggcttcaccg gggttgaggt ggtgcgcgaa cgcgtaggac aacccgagcg
      301 tgcccgccag ttcggcactg gcggccgagg aaccgagcag ccacaggtcc ggcacgttcc
      361 cgccgagcac gggaatcgcc ttgaccggcg cgtcttcctc gggatggaga aaggagcgca
      421 gctcgtcgag ttgctgctcg aagggcttcg ccgtccgctc gcgttcgggc cgcaccaccg
      481 ccaccgcgtg cggcgcgccg ccgggtgccc ggccgatgcc gagatcgatc cgccccgggt
      541 ggagcgcggc cagcgtgccg aactgctcgg ccaccacgat cggggcgtgg ttgggcagca
      601 gcaccccgcc cgcgccgaca cgcagccgcc gcgtggcgtt cgcgaggtgg ccgacgagca
      661 ccgcgggagc ggaactggcc accccgcgca tcccgtggtg ctcgggcacc cagtagcggt
      721 ggtaacccag cgcgtcggtc agcttcgcga gatcgacggt gtcacgcagc gcccgccccg
      781 gcgtcgaccc ccgcacgatc ggcgaggtgt ccagcaccga caagcgcaag ccgtccatcg
      841 ccgcccttcc gagaacgttt cgcggcggtc acccgactta ccggacatgg acaccgcacc
      901 cgacgccccg cccatcctgc ccgaagcggc cggcccgccg gcggaggcgc taccgcccaa
      961 acgcgggtcc gcgggtgccg gtctcaccat cgcgctggtg agcgggccgg tgtaccgcca
     1021 cccctggttc gaggacgcgg tggacgccgc ggtcgacgac gcgctgacct cgtgggggac
     1081 gatggccgcg ggcctggttc tgcagcgcgc tcccgcggca cgggtgctca actgctcgat
     1141 gtccgatcag gacaccatca ccgacggcca gatgctggct tcgctggcga aagcccgcgc
     1201 ggcgaacgcc gatctgatcg tcatcggttt cggcggggaa cccgccgacg caccgtcgct
     1261 gaccttcacc aaggtggtgg ggacgatcga gggactggtg ctggccgcgg cgggcagcaa
     1321 cggcacggcc gaccgcccgg accacccggc gaacgtgccg ggcgtggtgg cggtgggcgg
     1381 gttggtgcac gaccgggagg agggctggaa gcgcagcccg ttcagttcac cgggtgagat
     1441 ctacgccccc tgcgtcgacc tgctcaccac caccgtcggc gagaacggcc ccgggttcgc
     1501 ccggctgggc ggcaccatga tggccaccgc gctggtcgcc ggggagatcg cggcgaccat
     1561 gaccaggctg cgggtctccg cgggggaggc gctcgaacgg ctcaagcggc tgccgccgga
     1621 agtctttctc gaggcccacg aaggcaacgc ggcgggtcct ccgccgatct cggccgagcc
     1681 cgtggcgctg gccgggccga cggccggggt caacgactac tggaccacca ccgaccggct
     1741 gggctaccag ctgcacgccg acgcgctggc ctcgctcatt ctcgacgagg gcaccaagcc
     1801 accgctcacc gtcgcggtga agggggagtg gggcaccggg aagacctcgt tgctgcggat
     1861 gacgcaggca cggatcgacc cgctgctcga ctccggcgaa cgccgggcga tccggctgac
     1921 ccgcaccagc cacggcaagc tcgaccgggt cagttccggt gccccgcggc tggccacggt
     1981 gttcaagctg ctgcggcgcg cggaggtccg ggaggtggtg cgggacaagc cttcctcgtt
     2041 cggtacccgg ttgcgcacca ccgagctgcg cgcggagtcg accggccggt ggcggccgac
     2101 ggtctggttc aacccgtgga tgtaccagac cggggaacag gtctgggccg ggctcgcgca
     2161 cgagatcatc aaccaggtca cccagcgcct gccgctgatc gaccgcgagc gcttctggct
     2221 cgcgctgaac ctgagcaggc tcgatcgggt cgccgtgcgc gggcgggtcc tgcgcaccgt
     2281 gctggcccgg ctgctcccgg cgctggccac ctcggtgctg gcgttggtgg cgggcggtgt
     2341 cctgctcgcc tccggcaacg cgggctgggg cgcgggcgtg gcctcggccg gtgtgctcgg
     2401 ggtcgtcttc cggacggtgc gattgctgtg gtcccgcacc gatgcggtgc tgcccggcct
     2461 ggtgaccccg cccggtttcg ccggggtggt caccgggatc gccggtggga tcgcggacag
     2521 cgtcgacgat ccgcggtacc ggtcccgggc cggattcctc cacatggtgc acaacgacgt
     2581 cgcggccgtg ctggacctgg tcgccaccag ggaccgtccg ctggtggtct tcgtcgacga
     2641 cctggaccgc tgcacatcgg gcgcggtcat ccagctggtc gaggcgctga acctgttcct
     2701 ggccggtcag ttcccgaact gcgtgttcgt ggtggcgatg gaaccggagg tggtggcggc
     2761 gcggctggac gccgcctacg agccgtccgg ttccgggcac agcacgggat ggcggttcct
     2821 ggacaagctc gtgcaactgg agttcagcct gcccgcgccg gacgcggccg ccatgcaggg
     2881 ctacatcagc acgacgctga ccggttcgga agcgggcgag cccaccgggc cggggtcggt
     2941 gagcgaggag gccgtccggg cggcgcagcg ggtgctcggc tcgatgggtt cggtgcacga
     3001 cgtggccgac cgcgcgcgtg cgatcgaagg gccgggtacg gacgaacgct ccccggtgct
     3061 gtgggaggcc gccaaacggg agacgctgcg gcggctgacc ggcgacgatc ccgaggtccg
     3121 cgccattctc gcgacgatca gcgcccacct cggccgcaat ccccgggaga tcaagcgcgt
     3181 gatcaacctg atccgcttcc actcggtgat caatctgggc gcggcgcagc gcggcgggca
     3241 cacgccgttg tcgatctacc agctcaccaa gctggcgctg atcgccgtcc gctggccgca
     3301 cctgcgttcg gtgctcgccg gtcaggccgg gcggccgggc acgatcgtgc tcaccgcggc
     3361 ggaggacacc ctgcgcggcg cgcagggcgg atcgatccgc gaccggctgg tcggccaggg
     3421 cgtggacgcg gcgtcggcgg cggtgctcgg cgcgaacgcg gacctgctcc gcctgctcgc
     3481 cgacaatccg ccgatcgggc cgggagcggt ccggcacctc tgaccgcccg tggtcaggac
     3541 acggtgatgg ccgggctgaa caccgggttc tgcggcaccg tcgggtggag gtaacacgac
     3601 atcgtcaccg cgtgccactg gccggtggtc ggccggtgga actcgccggc gacgagcacc
     3661 gtcgaggacg agaccgtcgc ggtgccgggc gccccggcgg tcacggcggg cacggtcgag
     3721 gtcgccgact ggccgaacgg gatgaccatc gggccaccgg ccgggaagag cgtttccggc
     3781 acggtcaggt tgttggccgc cggcggggaa agcgtggcgc cggtggtggt gaggtagagg
     3841 tgcgcgctgc cgcggatgcc gtcccagccc gcggcggcga actgctcgtg cgtttccggc
     3901 gtgaaggtga tcgagccgcc caccccggtg ggcgtgaacg tggtgccgga cggcaccgaa
     3961 tccggcgcgt cgaaccgagc ttcgacggtg cccggttgcg gcggcaggct gggcagcgag
     4021 cagcggtaga ccaccggtgc ggccgtgccc gtgcggtagg tgatcgtggc ggcgaaggcc
     4081 gtcccggcgg tgcccagcac ggccaccgcg aaaacggcgg cggtggtggc aattcgagag
     4141 aacatcgcga ctccccggtc tcggttgccc ggaagctact gggcgcccgg ggccggtgga
     4201 aagacgcggg ggtgatcttg tcgaagagcg gtaaaaaccg ctcgccacgg tgtaccggag
     4261 tgacaactat cggcttcggc gagcgagagg tggcccggac gctatagtga acgccctatc
     4321 gcggaacgta cgttttaccg aacggaaggc cgggccgtgg agacctccga cccgtcggtg
     4381 cgggacctgc tggcggcgaa catcaagcgc gccagggcgg accgcgggct gtcgctgtcc
     4441 gagctgtccc gccgctcgtc gatcggcaag gccacgttgt cccagctcga gtccggcgcg
     4501 ggcaatccga ccatcgagac ggtgttcagc ctctcgcggg cgctggagct gcccatatcc
     4561 gacctgctcg agagcggtca ccacggcgac atgaccgtgg tgcgcggcga cgagcaggaa
     4621 gtgctcgccg gcgaggccgt ggaattgcgg atgctgcgcc ggatcgaagc cgacgggctc
     4681 atcttcgagg tctacgacca gcgcgtgcgg ggatcggccg gccagcgctc gctcggccac
     4741 gtcggcaccg agcacaccat cgtgcaggcg ggcaggctcc gcgtcgaggt cggcggcagc
     4801 accgccgaac tcggcccgag cgattcggtc gccttcgacg cctcgctccc gcacagctac
     4861 caggcgatcg gcggtgaggt gcgctcggtg ctgctgctcc agtaccgggg cggcagcagt
     4921 ccgcacgcct gactggcagg tgtgttgaca gtcgccccgc gcccggccta gcgttcggtt
     4981 caacgaacgg cggaggttcg atgtacggaa cgcgagtggt ggtgatcggc gccgggatcg
     5041 tgggggccgc ctgcgctcgc gaactgtgcg ccgaagggtt ccaggtgacc gtggtggacc
     5101 gcggccgtcc cggtggcgcg accaccgcgc acggggaagg caacgtcctg gtctccgaca
     5161 agggacccgg ggccgaactc gaacttgccc ggctctcacg ccgcctctgg cccgaagtgc
     5221 tcgctgaact gcccgccgcg gagggcgtcg aatgggacgc caagggcggg atcgtcgtgg
     5281 ccaccacgga atccggggcc cgcgcgctca ccgagttcgc cgagggccag cgcgccgccg
     5341 gaatcgtcgc cgagccgatg gacgccgccg cgctgggcga cgccgaaccg cacctgaccc
     5401 gggacgtgac cgccgcgatc cactacccgg aggacgcgca ggtgcaaccg gtcgccgcgg
     5461 cgaccgcgct gctcgccgcc gccggggtca ccctgtacac gcgctgcgag gtgaccggcg
     5521 cgctcaccga ttccggccgc ctgaccggga tccgcaccac cggcggtgat ctgcccgccg
     5581 acctggtggt gaacgcggcc ggtccgtggg cgggagagct gtcggcggtg ctcggcgtgc
     5641 cgatcgacgt gcggccgcgc cggggcgagg tgctggtgac cacgccgatg ccgccgacgg
     5701 tgttccacaa ggtctacgac gcggactacg tcggcgcggt cggcagcggc tcggccgaac
     5761 tgcagacctc cgcggtggtc gagtcgacga aggcgggcac catcctgctc ggttcctccc
     5821 gccgtcaggc cgggttcgac gaccgcttgc acctcggggc gctgtcggcg atcgcggcca
     5881 aggcgctgcg gttgttcccc ggtctcgcgg gcgtgtcggt gatgcgcgcc tacggtggtt
     5941 tccggcccta cgtcccggac catctgccgg tgctcggcgc cgacccgcgc ctgccggggc
     6001 tgtggcacgc gagcgggcac gaaggcgccg ggatcgggct gtccgtcggc accggccggg
     6061 tgctgcggga cctgatcacc gggcggcgcc cggaaatcga cgccggaccg ttccgggtgg
     6121 accgcccggc ggtgctggga ggtgctcgat gagcccgcgg ctggtgcccg gcgggacgga
     6181 ccgggtgcga cggcgggacc ggccgatccg gctgaccgtc gacggcgagc cggtgtccgg
     6241 tgtggacggt cagacgatcg cgggggtgtt gctggcggcg ggaagaaccg cctggcgcac
     6301 cgatccggcg ggtgagccgc gcggggtgtt ctgcggtatc ggtgcctgct tcgactgcgt
     6361 ggtcaccgtc ggtgccgaac gcgacgtgcg agcctgccgc cgccgggcgc gcgacggtga
     6421 cgaggtgcgc acccaggcac gggcggagga ccgatgaccc gcgtggtgat cgtcggggcg
     6481 ggacctgccg ggctggcggc ggccgacgcg gcgttgcgtg ccggggccgg ggtgagcctg
     6541 ctcgacgccg cggaacagcc gggcggccag taccaccgga tgctgcccgc cgcgttccgg
     6601 gccgagttac ccgacaaaat ccagcacggc cggcgggcct tcgaccggcg ccgccgtcgc
     6661 gtgctcgacc acccgcgctg cacgtggctg ccggaaacct cggtgtggtc actggaactc
     6721 cgcgacggcg agccgccgct ggtgcacgcg ctgcgcgggg tcgccgacgg cgccgaccgc
     6781 gagagcgtca ccctcgtgcc ggacgcgctc gtgctggcca ccggcgcaca cgatcgggtg
     6841 ctgccgttcc ccggctggac gctgcccggg gtgttcaccg ccggggcggc gcaggcgctg
     6901 gcgaagggcg agcggctggc cgtcggcgag aacgtcgtgg tgtccggcac cggcccgttc
     6961 ctgctcccgg tggcggcgtc actgtcgcaa gtggacgcac gggtgctggc ggtgttcgag
     7021 gccaaccagc cgcgaacggt cctgcgcggc tggacccgcg acgccgggga actggtgcca
     7081 cacctgaaga aggccgggga actcgcgggg tacgcggccg gtcagctgcg gcagcgcgtg
     7141 ccctaccgga tgggccgcgc ggtggtcgaa gcccgcggtg acggccgggt cgaagaggtg
     7201 gtggtggccc ggttgcgggc cgactggtcg atcatccccg gcaccgggcg cacgctcgcc
     7261 gcggacgcgg tgtgcgtcgg ccacgggttc accgcgcagc tcgaactgcc cgtggccgcc
     7321 gggtgcgacc tcgccggtga cgccgtcctg gtggacgcgg gccagcgcac cagccgtccc
     7381 ggggtgttcg ccgccgggga gatcaccggg atcgccggtg caccgtccgc tgtggacagt
     7441 ggaacggtgg cgggctggct ggccgccggg ggcgaccccg cccggatcac cggggcgctg
     7501 cgccgccggg accgcggccg cgccttcggg cggcggctgg cccgagcgca cccgatcggc
     7561 ggcggctgga ctggctggct gcgcccggac acggtcatct gccgctgcga agccgtcgac
     7621 cacgcctgcc tgaaccgggc actggccgcg ccggtcaccg ccggggcgcg ctcggtgaag
     7681 ctggccaccc gtgccggact cggtccctgc cagggccgga tctgcgggcc cgccgtcgcc
     7741 gaactgagcg ccgcggccgg tgccgaagtg gccaggccgc accaccggcc gatcgcgcag
     7801 cccatccggc tgggcgagct ggcgaacctg cgaacagagg agaacccttg agtaccaagg
     7861 ctgacctggg tggcgtcgtc gtggcgaccg cgctgccgta ccgggaagac ggacaagcac
     7921 cagccgggct cgcggtggac tacgaccggt acgccgagca ctgccgctgg ctgatcgaca
     7981 acggctgccg cggcatcggg cccaacggct cgctcggtga gtactcctcg ctcaccgacg
     8041 aagaacgccg ccgcgtggcg cgcaccgcga tcgaggcagt cggcgatgac gggatcgtcg
     8101 tggtcggcgt gcacggtgtc ggctcgcacc aggcgaagca ctgggccgaa ctcgccgccg
     8161 aggacggcgc gcacggggtg ctctgcctgc cgccgacgat gtaccgcgcc aaccgcggcg
     8221 aggtgctggc gcacttcgag gcggtggcct cggcgggact gccggtgatg gtctacaaca
     8281 acccgcacga caccaaggtc gacctgaccc cggacctgct cggcgagatc gcgcggatcg
     8341 agaacgtggt ggcggtcaag gagttctccg gtgacgtgcg gcgggtgctg gagatcaagg
     8401 agcacgcgcc ggacctggcg gtgatcagcg gggccgacga cgtggtgctg gaaatgctgc
     8461 tgatgggctc caccggctgg ttcgccggat tcccgaacgt gttccccgcc gaatcggtcg
     8521 agctgtacga cctggccacc cgtggcgaac tggcgcgggc ccgcgaactg tacgagccgc
     8581 tggtggcagc cttccgctgg gactcgcgca cggaattcgt ccaggcgatc aagctgggca
     8641 tggatcttgt cggccggtac ggcggcccgt gccgcccacc gcgcgggccg ctgatcccgg
     8701 cacacgacga acaggtgcgg gcggacatgg cgcgggcgat cgacgcgctg gcggggcggt
     8761 cctgatgcgc tcgatccgga cgctgagcgc ggtcgactcg cacaccgagg gcatgccgac
     8821 cagggtgatc accgggggag tggcgccgat ccccggcgac accatcgccg cgcgccgccg
     8881 ccacttcatc gaccacctcg acgacctccg gcggttcctg gtcgacgaac cacgcgggca
     8941 cccggcgatg agcggggcga tcctgcagcc gccgacccgg cccgacgccg actggggcgt
     9001 gctctacgtc gaagtcagcg gggtgctgcc gatgtgcggg cacggcacca tcggggtggc
     9061 cacggtgctg gtggagaccg gcatggtgcc ggtgaccgag ccggagacgg tggtccgcct
     9121 ggacaccccg gccgggctgg tgcaggccag ggtccaggtg cgcgacgggg tggccgagcg
     9181 ggtgaccctg cgcaacgtcg acgccttcgc cgtcgaactc ggtgccaccg tggacgtgcc
     9241 gggcctcggc gaggtccgct acgacatggc ctacggcggc aacttctacg ccatcgtgga
     9301 cctggagagc atcggcctgc cgttcgaccg ggcacgcaag aacgacatcc tggacgccgg
     9361 gctgcgcatc atggcggcgg tgaacgagcg caaccgcccc cggcacccgg tcgacgagca
     9421 catcggcggc tgcaagcacg tgcagttcct cgcgcccggc tcggacgcga agcactcccg
     9481 caacgcgatg gccatccacc ccggctggtt cgaccggtcg ccgtgcggca ccggcacctc
     9541 ggccaggatg gcgcagctgc acgcacgcgg ggaactgccg ctgcacaccg agttcgtcaa
     9601 cgaatccttc atcgggaccc ggttcaccgg gcagttgctc ggcaccaccg aggtcggtgg
     9661 cctgcccgcc gtcgtgcccg agttctccgg ccgtgcctgg atcaccggca cggcgaacta
     9721 cctgctcgac ccgcgcgacc cgttccccac cggattcgtg ctctgaatga tcagggtttg
     9781 ggcgtggtgc ccgtggtcgt tgccatcact ggagatcccc cttccggctg cctcggttcc
     9841 ccgtcaaggc aagcgggatc ccggggcgcc gggcaacgga tcggggtgtg cgggactgtc
     9901 ccggacaccg ctcagcggcc accgctgagc gtcgccagcg tctgcttcca ccgctggacg
     9961 accacggtga cgtcggcgtc cgggtcgacc acgcggctca tcgcggtgcc ggtcagcagg
    10021 tcgatggtga actgggccag cgtcggcaca cgcgggtcgt catcgcccac gtgcgcgatg
    10081 atcgcgtgcc gggcggcggt gatcacccgc tgctgtaccg gggcgagcgc ggcacgcagc
    10141 tcgggatcgg tgcgcgaggc gacccacagt tccagcgccg cggcgaacgc cgggctctgc
    10201 aggtcgcgcc agaccaggtc gaccaccccg tcccagccgc catcggccgg cggaccggcg
    10261 gtgccggaac tgaggacccg gacccggcga tcggcgatgt cgtcgaccac cgcggccatc
    10321 agcgtcgcgc gcgtggggaa gtggtgcagc agtgtgccgc gcgccactcc ggcgcgctgc
    10381 tgcaccgcgc cgaccgtggt ggcggaatag ccgccttcca gcaagcaggc gtaggccgcg
    10441 tcgatcaacc gcgcgcgcgt ggccgccgcg cggtcctgcc ggggagggcg gagtgtggac
    10501 accgggtcac tctaatggca ccatcgcaaa acagtctgat cggactgctt tgtggaaggt
    10561 gatcgatgag caggtggcgc acggcccgca cggtcgcgac cgcactggtg cgcaacgcgt
    10621 tcacgcgctc gggtggcgcc acgagagcgg acctgccgga ggtgcccgcc ccggccggga
    10681 tcgacggcta cgcccgccac gtcaccgcga gtggtgcggt cgcggccgag cccgccgcgg
    10741 tggtcgaact cgcccgcgac ctcgaacgca cccacgaatg gctcacgctg cacgcggcct
    10801 ggcgcggcga gcggccggag cggatcgaac cgggcgcgga gttcacccag cagatccggc
    10861 tgatggacat ccccgcccag gcgagatggg tggtcgagca ggcggacgag cacgggttcg
    10921 ccctgcgcgg gaccgggccg atgggcatca cgctcggcct gtggtgcacc gtgcggccgg
    10981 ctccgggcgg cacggtcctc cgcctggacg gagcgctcga cggggcgccg gtgcgcgggc
    11041 cggtcggttc gaccgccgtg cacagcgtcg aacgggcgct cgccgagtcc gtttccgcgc
    11101 tcgccgagct ggtccatggc acgaaggggc ggcggctttc cgtgtcgtcc gaaccgatcc
    11161 gccatgagcg gaccggcacg ctgatcgacc cgcgcgcccc ggtcgtggtg ggagtcgggc
    11221 aggtggtgaa ccggaagccg gacctcgacg cgccccgtga gccggtcgat ctcgccgccg
    11281 aggcgttgcg cgcggcggcc gccgacgcgg gcgctgatct gctggcgcag gccgatctgg
    11341 tctacgcggt gccgagcgcg tcctggagct accccgacca ggcgggtgcg gtcgccgagc
    11401 gcgtcggcgc tacccgggcg accaccgtgc agaccagcgc ccagggcggc gacggcggcc
    11461 agctcgcgat caacgacgcc gcacaggaaa tcgtcgacgg ccacgcggag atcgccctga
    11521 tctcgggagc ggaagccggg gccacgatgg ccgcgctcac gcgggatccc ggctggaccc
    11581 ggcaaccgga gaaagccgct cccgaccgga agatcgggca ggaccggccc gccaacaacg
    11641 aggccgagac ggcggttggc ctcggtgcgc cgatcttcgt ctacccgctg ctggagtcgg
    11701 ccctgcgcgg ccgggccgga accgcgcccg acgagcaccg cgcccgcatc gccggactct
    11761 ggtcgcggtt gtcgcaggtg gccgcctcga acccgtacgc gtgggacccg accgcgcgca
    11821 cgccggagga gatcgccacc ccgaccgggc gcaaccgccg ggtcagcgac ccgtacacca
    11881 agctcatgtg cgccaacctc caggtcgatc tcgccgcggc ggtgatcgtg accagcgcgg
    11941 gggcggcgca cgcggcgggc attccgcagg agcgctgggt ttttgtgcac gccggcgcgt
    12001 cggccaccga ggagtggttc gtctccgaac gggccgagct ggccgaatcc ccggccatcg
    12061 cggcggccgg ggccgccgcg ctcgggcacg ccgggatcac cgcggccgac ctcggcccgg
    12121 tcgacctcta ctcgtgcttc ccggccgcgg tccagctggc ggcgaacgcg ctcggcctgc
    12181 cgtgggacga cccggaccgg ccgctgagcg tcaccggcgg gctcacctcg gcgggcggcc
    12241 ccggcaacaa ctacgggacg cacgcggtgg cgtcgatcgt gccgctgctc cgggacgcgc
    12301 cggccgaggt cggcctcacc acctcgctcg gctggtacgc gacgaagcat tccgtgggcg
    12361 tctattccgc gaccccgccg gagaaggcgt tcgcgcacct gcgcccggcg ttcccgcgcc
    12421 cggccacgcg gcccgtgcgg accgaactgg acggtagtgg ggtggtggaa gcggccaccc
    12481 aggtctgccg tcccgacggc gagccggaag cggtgctgct gagcgtgctc accgacgacg
    12541 gcgcccgggt gctgctgcgg caggaagggg cgcaggaggc cgatctcctt cgctgccgtg
    12601 tccgcctcgt cgacgggcgg atcgaggtcg tcgacgacaa ccgtcaggag ttgcccccgc
    12661 ctccggccgc gcccgtgcgc accgagcgcc ggggaccgga cggcgagatt tcggtcatca
    12721 cgctcgaccg cccgccggtg cgcaacgcgg tgaaccggcg catggcgctg gcgctggagc
    12781 aggcggtgga cgacgccgaa gccgatccgt ccgtgcgcgt gctggtcctg accggcgccg
    12841 ggggcacttt c
//
