LOCUS       NZ_CP045480            11827 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Amycolatopsis sp. YIM 10 chromosome, complete genome.
ACCESSION   NZ_CP045480
VERSION     NZ_CP045480.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. YIM 10
  ORGANISM  Amycolatopsis sp. YIM 10
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Complete genome sequence of Amycolatopsis sp. YIM 10, isolated from
            a rare earth mine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2019) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009429145.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 04:03:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,775
            CDSs (total)                      :: 9,711
            Genes (coding)                    :: 9,634
            CDSs (with protein)               :: 9,634
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 22 of 77
            Pseudo Genes (incomplete)         :: 65 of 77
            Pseudo Genes (internal stop)      :: 1 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:01:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3416683
            Orig. end                         :: 3428510
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045480.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11827
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11827
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11827
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6827](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6827)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            586..2205
                     /locus_tag="YIM_RS16670"
                     /old_locus_tag="YIM_16800"
     CDS             586..2205
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152441.1"
                     /locus_tag="YIM_RS16670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16800"
                     /product="alkaline phosphatase"
                     /protein_id="WP_153031222.1"
                     /transl_table=11
                     /translation="MKSASSVRTGDPEVTISRRQLLGYGGAGIGAVLLGNGMWNAASAF
                     AAPPGTSNPFTLGVASGDPAPDGVVLWTRLAPDPFAVDGAGGMPAKPVRVEYELARDER
                     FRHVVRRGSVVATPELGHSVHPEIHGLAPDREYHYRFRVGGEVSPVGRTRTMPSVRANP
                     NELRFAFASCQAWQDGYFTAYEHMAAEDLDFVVHLGDYLYESGVGTNKRGVTVDPRFHT
                     ETFDLARYRLQYSLYKMEAPLQLAHARFPWIVTFDDHEVENNWAGDLSQADTEPDQDPA
                     VFRQRRAAAFQAMYENLPLRRAQMPSGADIRMHRRLKYGRLADLTMIDTRQYRDDQPCG
                     DGGGVDCADRFDTDRTILGASQREWLLDGFSQSDARWQILGNQAPLGQTDRDPGPAVDV
                     WYDPWDGYVADRDRVLAAAQDRDVRNLVVITGDRHQNYAWDLKRDFTDPDSPVVASEFV
                     GTSVTSGADGADMNDDGVKFLAANPHMKFFNAQRGYVRVDVNRDRWRSDFRVLPYVTKP
                     GAPISTRASYVVEDHRPGVQPA"
     gene            complement(2234..2563)
                     /locus_tag="YIM_RS16675"
                     /old_locus_tag="YIM_16805"
     CDS             complement(2234..2563)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331293.1"
                     /locus_tag="YIM_RS16675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16805"
                     /product="NIPSNAP family protein"
                     /protein_id="WP_153031223.1"
                     /transl_table=11
                     /translation="MITCHLRYEIDPSQAVAFETYVTGWIPIVTRFGGTHHGCFLPHEG
                     ANDIAYALFSFPSLAAYEDYRAAIRQDPEAQRLWQLSTETRCIRRFDRTFLRPAMQPSG
                     GVEAA"
     misc_feature    2527..2541
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 21.9"
                     /tool="antismash"
     gene            complement(2601..3227)
                     /locus_tag="YIM_RS16680"
                     /old_locus_tag="YIM_16810"
     CDS             complement(2601..3227)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077081.1"
                     /locus_tag="YIM_RS16680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16810"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_153031224.1"
                     /transl_table=11
                     /translation="MSTWLTDTRTSYDTVADDYASQLRDAVAGDVYLRGALAMFAEQVR
                     EGPVADIGCGPGHVTAHLAGLGVEAFGIDLSPAMIEVARRDHPGLRFAVGSMTELDLAP
                     GSVGGVLAWWSLIHLPDDEIPLVLGHFRRVLRQGGPLLAGFHVGDDSRLKTEGYGGHPM
                     KVHVHWRQPDQMATWLRNAGFTVEAQVLLKPDESVPQALLFAVAA"
     gene            complement(3190..4431)
                     /locus_tag="YIM_RS16685"
                     /old_locus_tag="YIM_16815"
     CDS             complement(3190..4431)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /locus_tag="YIM_RS16685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16815"
                     /product="MFS transporter"
                     /protein_id="WP_194240172.1"
                     /transl_table=11
                     /translation="MAPGLLRAHPVFRSVLAARAVSALGDRFSLVAVPVLALSSAGATP
                     GQVALLWTVQLLPGAVLALPAAANLVGRPERAAMIACDLLRAVLLLGVFGLAAGGELRL
                     WHLFAVVPAIGVLTTVFDVGAQAYVPRIVDRADYAEANSRFTQAESAADVAGPLVAGVL
                     IGFAGAGTALLVDAVSFVLSAVLLAPIRAVHDQPVEVAASGWWRNLRDGIRFVAGSPVL
                     RSLVAAFALFNFGGAVVSSLWFAYLLDHLRLPPSVTGGLITVGGVSGLVAALATRRVLG
                     VVSSRVVLPVSLVVIVVSLWFVPLAALGSPVLLLAAYQALFGAGVAFFAVTAATVRQLL
                     TPVGQQGRVYAVFYTFSLLTVPIGGLLAGVLASSFSPVVAIVAGAAIAATSLLTLVSYR
                     SGNVDLADRHPDLL"
     misc_feature    complement(3874..3876)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4434..5000)
                     /locus_tag="YIM_RS16690"
                     /old_locus_tag="YIM_16820"
     CDS             complement(4434..5000)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="YIM_RS16690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16820"
                     /product="DUF5825 family protein"
                     /protein_id="WP_153031226.1"
                     /transl_table=11
                     /translation="MDVSIDPRVRHVGPAEILALDPAALSADGVQLLVLPEPVSFGSDD
                     ERDVRFLRLLREAVSYTLRIEWRARAVPFDLMLVGHLPPPLGDEGREWRHAYRFGLCYY
                     QRGPGFVLLKDVRGEGGARYRIDDEKAMSAWPELEPVRRWSELSPAAQELCALLAEEEL
                     LLRRGDWVTLLPYRLRRWPVPFDAI"
     gene            complement(5001..6827)
                     /locus_tag="YIM_RS16695"
                     /old_locus_tag="YIM_16825"
     CDS             complement(5001..6827)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="YIM_RS16695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16825"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_194240173.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.2e-170, bitscore:
                     565.6, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4.9e-14, bitscore: 52.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLNVRLAAMPWQALDMPSLPLGLLRASCRAAGREAPPCYHGNM
                     HWAEFLLERSDGELGPNEYLEVAENGIFHGIGDFVFTGVLHGGDFGLAEFTDLLRRRGF
                     DPGRALDMRPHAEEFIDLAAREILADEPDLVGFSSTFMQNVPSLALAARIKQYAPGVLT
                     VLGGGNCDGPMGAALHRNYPCLDYVVRGEGEEIFPALLDAIERGAEPADLPGLCWRRDG
                     KSVANPDRREPLPPGRIPMPDFDDWFELIESSPLEGHVQPKLVLESARGCWWGELHHCT
                     FCGLNGSLMEFRSKAPERVLGEISHLVARHRTLDIIMVDNIIDNAYFTTLLPDIAALDW
                     DLRIHYEVKANLTPAQIGALRDARVAHLQPGIESLSTRVLKLMDKGVDAIRNVRTLRDC
                     HSAQLDTTWNLLFGFPGETDDDYWPLIRQLPALVHLQPPSDATPILLERFSPNFDRPEL
                     GFGRRRPAEIYRHVYDLPQRELEDLVYLFDSEELGILGEVVEALEEAIEEWKKRHHESS
                     LLRVDFDGGILLEDRRSGWPVRDHHIEDPALVAAYRELEKGRSNRALLSKVDVPAQRLE
                     AWLDELCDAGLVFREGDRFIALATTSVPVRIK"
     gene            complement(6982..7740)
                     /locus_tag="YIM_RS16700"
                     /old_locus_tag="YIM_16835"
     CDS             complement(6982..7740)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="YIM_RS16700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="YIM_16835"
                     /product="ferritin-like domain-containing protein"
                     /protein_id="WP_153031227.1"
                     /transl_table=11
                     /translation="MTLLKPEITTLDDASFQLFASGRHSEQWSAREIIGDGATELDEAD
                     TERAWILASTSYFAEQAGLVAAAELVSETDDVALRLSFATAVSDEARHADAFLAYAVAR
                     GGDLANVSEDGFLDELHETLSTVSHLEKFLMHTTFEGIAADEFVLLQRIFAGDPLGEIY
                     RHVRSDEVRHVAIGLNYLRRSYATPEGREEWDTHFGEWSERALRIARLPEVAAGLGAVM
                     GKPAEPIEQWFMRRHRARLRGAGIPVPERR"
     gene            complement(7737..8684)
                     /locus_tag="YIM_RS16705"
                     /old_locus_tag="YIM_16840"
     CDS             complement(7737..8684)
                     /EC_number="1.2.4.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /locus_tag="YIM_RS16705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16840"
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="WP_153031228.1"
                     /transl_table=11
                     /translation="MIALHAALVQALRRDPDLVYLCTWQPGLFVDEFGPERIRSMPIAE
                     NATLDFAVGLAAAGLRPVVDLARAAFLFTAMDPLVNQATKLRYLTGGQYEVPLTVRAQT
                     RGGGESLGAQHEHLPYAMLAQVPGLVVAVPGSGAEAAGLLATAMTHPDPVVILESPALF
                     TDEPHWTGGAVDGVPFGRVRIARRGKDITIVAIGLGVRHALTAADRLAEFGVSAMVVDV
                     RTVAPLDIAGLAESAAATGTVLVVDEAIGPCSVASEIVANLVTAEVVRPRQARRCTALP
                     VPSPFSPALQRAVYPGPASIERQALALLGERSPA"
     gene            complement(8689..9648)
                     /locus_tag="YIM_RS16710"
                     /old_locus_tag="YIM_16845"
     CDS             complement(8689..9648)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /locus_tag="YIM_RS16710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16845"
                     /product="thiamine pyrophosphate-dependent dehydrogenase E1
                     component subunit alpha"
                     /protein_id="WP_194240174.1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 7.1e-06, bitscore:
                     25.0, seeds: 323, tool: rule-based-clusters)"
                     /sec_met_domain="DXP_synthase_N (E-value: 1.9e-06,
                     bitscore: 26.4, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLADAATAVQRRDLPLYETMLRIRAFEETALELQRRGKVPGALH
                     PATGHEAIAAGVCARLGPADHVVSYYRCHGHALACGLDPAAMFAELLGRATGLNKGKGG
                     SMHLADRSHRFLGGNSIVGANVGIAAGMAAALRIRKEPGAVVVFFGDGAVGAGVVLETL
                     RIAAHQRLPLLFVCENNGYQDRTRSELVSGIAPGAVAAGLGVPSVSVDGNDVRAVAGTA
                     AELLPLVRDERTARFLDASTYLRDFHCQFGPEPPGEYRPLDEVRQWRARDPLSRADFDL
                     TEPRRRAAEEMRLAAAHALTQPEPSPGTVCDDLTVARW"
     gene            complement(9744..10892)
                     /locus_tag="YIM_RS16715"
                     /old_locus_tag="YIM_16850"
     CDS             complement(9744..10892)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070894.1"
                     /locus_tag="YIM_RS16715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16850"
                     /product="CdaR family transcriptional regulator"
                     /protein_id="WP_153031230.1"
                     /transl_table=11
                     /translation="MSDLFGVLAKRAEANARAAVETFIREIPDYRREAANPHGKAELLD
                     YAVWFRRHTVEMARTGEPLGESALSFIGAIGRSRAAGGFTTRSVESALTLHTNLMLSEI
                     QAAAGESADVTELLRLTAWFGAQGLRGCAAYLNGYLDEQALRVKPAQRVRELAESLLAN
                     ATTTAEIARRLGVEAHAEYFVAVLRFAAKSIGAADEIIEAAFRRHSVVTTWHEPDELVA
                     LLPVDHDRALAVVREMVAAAGVPCAVGAATGHAGALAEAAEVARRVADVAPRERVPSTL
                     YGIPEVFVELGVAGVPEIDHWLGELVRRLAGGPELVATLDAYYRNDMNRLGAALALHIH
                     PRTLDYRLRRVRELVGVDPGSVRGVRILTSAVARAAHRGCHT"
     gene            11217..11543
                     /locus_tag="YIM_RS16720"
                     /old_locus_tag="YIM_16855"
     CDS             11217..11543
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667550.1"
                     /locus_tag="YIM_RS16720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_16855"
                     /product="hypothetical protein"
                     /protein_id="WP_153031231.1"
                     /transl_table=11
                     /translation="MDDPHIHVERKVLHSGADYRNAVASMLGAVTDAPAVVTTGCGIEV
                     PYAMTSPHPESVTCLACREHAAREHRAFAAQVEKLSRMPGSVVAPEDAAKAVERHQDLA
                     RRFG"
ORIGIN
        1 ggacggtcgc ggggctggcc ccggcggcgc cggggtaccg gcggatcacc gtgcgcccgg
       61 tgccggacgc cgcgctgacc cacgcctccg cgcggcacga aaccccgtac ggcacggcgt
      121 cggtgtcgtg gcgtcgggac ggcggtgagt tcactgtgga ggttgagatc ccggcgggtt
      181 cgtcggccga ggtgcacctt cccggccgtg ctccgatcga ggtgcggcac ggccgtcacg
      241 agtggacggt gccggatccg gtgacgccgc gcccggttcc ggtgaccgtg cgggacctgc
      301 tcgaccacgc ggaactgtgg ccgaaagcgg tggcgctgct gggagaactg ggtctcggtg
      361 agggttcggc gcagatcgcc aaggaagcgg cgccgttcct ggaccggccg gccgccgaac
      421 tcgcgggcct gctcgcgatg aaggacccgt cccgcgacgg gtccgccgcc gaagccgccc
      481 tcgccgcgct gctcgggtga cgtctgccgt tccatccggc gtcggcgacg tgaacagggc
      541 ctgtacttcg ggcagaaccc ttgttgcgcg ccaacggccc gggttatgaa atccgcgagt
      601 tcggtgagga caggagatcc tgaagtgacg atttcgcgtc ggcagctgct cgggtacggc
      661 ggagccggga tcggggcggt gctgctcggc aacgggatgt ggaacgcggc gagcgccttc
      721 gccgcgccgc cgggaacgtc gaacccgttc accctcgggg tggcgtcggg tgatcccgcg
      781 cccgacgggg tggtgctgtg gacgcggctg gcgcccgatc cgttcgctgt ggacggtgcg
      841 ggtggcatgc cggccaagcc ggtgcgggtg gagtacgaac tcgcgcggga cgagcggttc
      901 cggcacgtgg tgcgccgcgg cagcgtggtc gcgacgccgg agctgggaca ctcggtgcat
      961 cccgagatcc acggcctggc accggatcgc gagtaccact accggttccg ggtgggtggc
     1021 gaggtttcgc cggtgggccg taccaggact atgccgtcgg tgcgggcgaa tccgaacgag
     1081 ttgcggttcg cgttcgcgtc gtgccaggcg tggcaggacg ggtacttcac cgcgtacgag
     1141 cacatggccg ccgaggacct ggacttcgtg gtgcacctcg gtgactacct ctacgaatcc
     1201 ggggtcggta cgaacaagcg cggcgtgacg gtggacccgc gattccacac cgagaccttc
     1261 gacctggccc ggtaccggct gcagtactcg ctgtacaaga tggaggcgcc gctgcagctg
     1321 gcccacgcgc ggttcccgtg gatcgtcacc ttcgacgacc atgaggtgga gaacaactgg
     1381 gcgggcgacc tttcccaggc ggataccgag ccagaccagg accccgcggt gttccggcag
     1441 cggcgagcgg cggcgttcca ggcgatgtac gagaacctgc cgctgcgacg ggcgcagatg
     1501 ccgtcgggcg cggacatccg catgcaccgg cgcctgaagt acgggcggct ggcggatctc
     1561 accatgatcg acacgcggca gtaccgggac gaccagccgt gcggtgacgg aggaggcgtc
     1621 gactgcgccg accggttcga caccgaccgc acgatcctcg gcgcttcgca gcgcgagtgg
     1681 ctgctcgacg gcttcagcca gtcggacgcg cggtggcaga tcctgggcaa ccaggccccg
     1741 ctcgggcaga ccgaccgcga tccgggcccg gcggtcgacg tctggtacga cccgtgggac
     1801 ggctacgtgg ccgatcgcga ccgggtgctg gccgccgcgc aggaccggga cgtgcggaac
     1861 ctggtggtga tcaccgggga caggcaccag aactacgcct gggacctgaa gcgggacttc
     1921 accgacccgg actcgccggt ggtggcgagc gagttcgtcg gcacctcggt gaccagcggg
     1981 gccgatggcg cggacatgaa cgacgacggc gtgaagttcc tcgcggcgaa cccgcacatg
     2041 aagttcttca acgcccagcg gggttacgtg cgggtcgacg tcaaccggga ccgctggcgc
     2101 agcgacttcc gcgtcctgcc ctatgtcacg aagccgggcg cgcccatctc gacccgcgcc
     2161 agctacgtcg tcgaagacca ccgtcccggc gtccaaccgg cctgacgctc caggacaccc
     2221 gtctgcgagc gggtcaagcg gcctcgacgc cgcctgacgg ttgcatcgcc gggcggagga
     2281 aggtccggtc gaagcggcgg atgcagcggg tctcggtgct gagctgccag agccgctgag
     2341 cttccggatc ctgccggatg gcagcccggt agtcctcgta ggcagccagg ctggggaagg
     2401 agaacagggc gtaggcgatg tcgttggcgc cttcgtgcgg caagaagcag ccgtggtgcg
     2461 tgccgccgaa gcgggtgacg atcgggatcc accccgtcac gtacgtctcg aacgcgacag
     2521 cctggctggg atcgatctca tagcgcagat gacaagtgat cacgtgagtt tttccttctg
     2581 gcccggtagt tctggtatcg ctaagcggcc acggcgaaga ggagggcttg cggaacgctc
     2641 tcgtccggct tgagcagcac ctgggcctcg acggtgaatc ccgcgttccg cagccaggtc
     2701 gccatctgat cgggttgccg ccagtggacg tgcaccttca tcggatggcc gccgtacccc
     2761 tcggtcttca ggcgtgaatc gtcgccgacg tggaagcccg ccagcaacgg cccgccctgg
     2821 cgcagcacgc gccggaagtg cccgagcacc aaggggatct cgtcgtccgg gaggtggatc
     2881 aacgaccacc aggcgagcac gccgcccacc gagccggggg cgaggtccag ctccgtcatc
     2941 gagccgaccg cgaagcgcag gccggggtgg tcgcgccgcg ccacctcgat catcgcgggg
     3001 gagaggtcga tgccgaacgc ttcgaccccc agcccggcca ggtgggcggt gacgtgcccg
     3061 ggaccgcagc cgatgtccgc gacgggcccc tcgcgcacct gctcggcgaa catcgccagc
     3121 gcgccgcgca ggtacacgtc accggccacg gcgtcccgca gttgcgaggc gtagtcgtcg
     3181 gcgacggtgt cataggaggt ccgggtgtct gtcagccagg tcgacatttc cggaacgata
     3241 gctgacgagg gtgagcaggc tggtggcggc gatcgccgct cccgccacga tcgcgaccac
     3301 cggggagaag gacgaagcca gcacgccggc cagcaacccg ccgatcggca cggtcagcag
     3361 ggagaacgtg tagaacaccg cgtacacccg tccctgctgc ccgaccgggg tcagcagctg
     3421 cctgaccgtg gcggcggtga cggcgaagaa cgcgactccc gcaccgaaga gtgcctggta
     3481 ggcggcgagc agcaacaccg gcgacccgag cgcggccagc ggcacgaacc acagcgaaac
     3541 cacgatgacc accagcgaaa ccggcaacac cacccgtgag ctgaccacgc ccagcacccg
     3601 ccgggtggcc agcgccgcga ccaggccgct caccccgccg acggtgatca gcccgccggt
     3661 caccgaaggc ggcaaccgca ggtggtcgag caggtaggcg aaccagagcg agctgaccac
     3721 cgcgcccccg aagttgaaca acgcgaacgc cgccaccagc gaccgcagca ccgggctgcc
     3781 cgccacaaaa cggattccgt cacgcaggtt ccgccaccaa cccgacgcgg ccacctccac
     3841 cggctggtcg tgcaccgcgc ggatcggggc cagtaacacc gccgacagca cgaagctcac
     3901 cgcgtccacc agcaacgccg tgcccgcgcc cgcgaagccg atcagcacac ccgcgaccaa
     3961 cggccccgcc acgtcggcgg cgctttccgc ctgggtgaac cggctgttcg cttcggcgta
     4021 gtcggcgcgg tcgacgatcc ggggcacgta agcctgcgca cccacgtcga acaccgtggt
     4081 cagcaccccg atcgccggca ccaccgcgaa caggtgccac aaccgcagct caccaccggc
     4141 ggccaggccg aagacgccca gcagcaacac cgcccgcagc aggtcgcagg cgatcatcgc
     4201 ggcccgctcc ggtcggccga ccaggttcgc ggcggcgggc agggccagca ccgcgccggg
     4261 cagcagctgc accgtccaca gtagagccac ctggcccggc gtcgccccgg ccgaggacag
     4321 ggccagcacc ggcacggcga ccagcgagaa ccggtcaccc agcgcgctga ccgcccgtgc
     4381 cgcgagcacc gagcggaaca ccgggtgcgc gcggagcagg cccggcgcca cggtcagatc
     4441 gcgtcgaacg gcacgggcca gcggcgcagc cggtacggca gcagcgtcac ccagtcaccg
     4501 cggcgcagca gcagttcttc ctcggccagc aacgcgcaga gctcctgcgc cgccggggaa
     4561 agctcggacc aacgccgtac cggttccagt tccggccacg ccgacatcgc cttctcgtcg
     4621 tcgatccggt accgcgcccc gccctccccg cgaacgtcct tcagcagcac aaaacccggg
     4681 ccgcgctggt agtagcaaag gccgaaccgg taggcgtgcc gccattcccg gccctcatcc
     4741 ccgagaggag gcggcagatg ccccaccaac atcaggtcga acggcaccgc ccgcgcccgc
     4801 cactcgatgc ggagcgtgta cgacaccgcc tcccgcaaca accgcaggaa acgcacgtcc
     4861 cgctcgtcgt ccgaaccgaa gctcaccggt tcgggcagga ccagcaactg gaccccgtcg
     4921 gcggacaacg ccgccggatc gagcgcgagg atctccgccg ggccgacgtg gcgcacccgt
     4981 ggatcgatcg aaacgtccat ctacttgatc ctcaccggaa cgctggtggt ggcgagcgcg
     5041 atgaaccggt cgccctcgcg gaagaccagg cccgcgtcgc agagttcgtc gagccacgct
     5101 tcgagtcgtt gggcgggaac gtccacttta gacagcaaag ctcggttcga ccgtcccttc
     5161 tccagttccc ggtaggcggc gaccagcgcg gggtcctcga tgtggtggtc gcgcaccggc
     5221 cagccgctgc gccggtcctc cagcaggatc ccgccgtcga agtcgacccg cagcagactg
     5281 ctttcgtggt gccgcttctt ccactcctcg atggcttcct cgagcgcctc gaccacttcg
     5341 ccgagaatgc cgagttcctc gctgtcgaac aggtagacca ggtcttccag ttcgcgctgc
     5401 ggcaggtcgt agacgtgccg gtagatctcg gccggccgcc gccggccgaa gcccagttcc
     5461 ggccggtcga agttcgggct gaaccgttcg agcaggatcg gcgtggcgtc cgacggcggc
     5521 tggaggtgca ccagcgcggg cagctggcgg atcagcggcc agtagtcgtc gtcggtctcg
     5581 ccgggaaagc cgaagagcag gttccaggtg gtgtcgagct gcgcggaatg gcagtcgcgc
     5641 agggtccgca cgttccggat cgcgtccacg cccttgtcca tcagcttcag cacccgcgtg
     5701 ctgagcgatt cgatgccggg ctggaggtgc gccacccgcg cgtcgcgcag cgcgccgatc
     5761 tgcgccgggg tgaggttcgc cttgacctcg tagtggatgc gcaggtccca gtcgagcgcg
     5821 gcgatgtcgg gcagcagcgt ggtgaagtag gcgttgtcga tgatgttgtc caccatgatg
     5881 atgtcgagcg tgcggtgccg ggcgaccagg tgcgagatct cgccgagcac ccgctccggc
     5941 gctttgctgc ggaactccat cagcgagccg ttgagcccgc agaaggtgca gtggtgcaac
     6001 tcgccccacc agcagccgcg agccgattcg agcaccagct tcggctggac gtggccttcc
     6061 agcggggagc tctcgatcag ctcgaaccag tcgtcgaagt ccggcatcgg gatgcgtccc
     6121 ggcggcagcg gctcgcgccg gtcgggattg gcgacgcttt tcccgtcacg gcgccagcac
     6181 aggccgggca ggtccgcggg ctccgcaccc cgttcgatcg cgtccagcag cgccgggaag
     6241 atctcctcgc cctcaccacg caccacgtag tccaggcacg ggtagttgcg gtgcagcgcg
     6301 gcgcccatcg gcccgtcgca gttgccgccg ccgagcacgg tcaatacccc tggggcgtac
     6361 tgcttgatcc gggcggccag cgcgaggctc ggcacgttct gcatgaacgt gctgctgaat
     6421 ccgaccagat ccggttcgtc cgcgaggatc tcgcgcgccg ccaggtcgat gaactcctcg
     6481 gcgtgcggcc gcatgtccag cgcccggccc gggtcgaaac cccggcgtcg caacaggtcg
     6541 gtgaactcgg ccaggccgaa gtcgccgccg tgcagcacgc cggtgaagac gaagtcaccg
     6601 atcccgtgga agatgccgtt ctcggcgacc tccaggtact cgttcgggcc gagttcgcca
     6661 tcggaccgct ccagcaggaa ctcggcccag tgcatgttgc cgtggtagca gggcggtgcc
     6721 tcgcggccgg cggcccggca cgacgcccgg agcaggccca gtggcagcga gggcatgtcg
     6781 agggcctgcc acggcatcgc ggccagccgg acgttgaggg tgctcatcgg gcctccagac
     6841 cggagtgggg ggcgcggtgc acgcccccca cgggtgggtc agtgcttctt cacggttcgc
     6901 gcctgcgtct tgatcggcac caggtaccgg caggtgccgg tcaccgactt ctcgagccgg
     6961 acctttttga tcgccatgtg ttcacctcct ctccgggacg gggattccgg ccccgcgcag
     7021 gcgtgcgcga tgccggcgca tgaaccactg ctcgatcggc tcggccggct tgcccatgac
     7081 cgcgccgagt ccggcggcca cctccggcag ccgggcgatc cgcagcgcgc gctcggacca
     7141 ctcgccgaag tgggtgtccc actcctcgcg gccctccggg gtggcgtagc tgcgccgcag
     7201 gtagttcagc ccgatcgcga cgtggcgcac ctcgtccgag cggacgtgcc ggtagatctc
     7261 gccgagcggg tcaccggcga agatccgctg cagcagcacg aactcgtccg ccgcgatgcc
     7321 ctcgaaggtg gtgtgcatca ggaacttctc cagatggctc accgtggaca gggtctcgtg
     7381 cagttcgtcg aggaagccgt cttcggagac gttcgccagg tcaccgccgc gggccacggc
     7441 gtaggcgaga aaggcgtcgg cgtgcctggc ttcgtcggag accgcggtgg cgaagctcag
     7501 gcgcagcgcg acgtcgtcgg tctccgacac cagttccgcg gcggccacca gcccggcctg
     7561 ctcggcgaag tacgaagtgg acgccagtat ccacgcgcgc tcggtgtcgg cctcgtcgag
     7621 ttcggtggca ccatcgccga tgatctcgcg cgccgaccac tgctcgctgt gccgcccgct
     7681 ggcgaagagc tggaacgagg cgtcgtccag ggtggtgatc tcgggtttga gcagggtcat
     7741 gccggtgacc tttcgccgag cagggccagt gcctgccgtt cgatggatgc ggggccgggg
     7801 tagaccgccc gctggagcgc cgggctgaac ggggacggca ccgggagcgc ggtgcagcgc
     7861 ctggcctgcc gcggccgcac cacctccgcg gtgaccaggt tcgcgacgat ctccgaggcg
     7921 accgagcagg ggccgatggc ttcgtcgacc accagcacgg tgccggtcgc cgccgccgat
     7981 tcggcgagcc cggcgatgtc cagcggcgcg accgtccgca cgtcgaccac catcgcgctc
     8041 acgccgaact cggcgagccg gtcggccgcg gtgagcgcgt gccgcacgcc gagtccgatc
     8101 gcgacgatgg tgatgtcctt gccgcgcctg gcgatccgga cccggccgaa cgggacgccg
     8161 tcgaccgctc cgccggtcca gtgcggttcg tcggtgaaca acgcgggtga ctccaggatg
     8221 accaccggat cggggtgcgt catcgcggtc gccagcaatc cggccgcctc ggcaccgctg
     8281 ccgggcaccg cgaccaccag gccggggacc tgggcgagca tcgcgtacgg caggtgctcg
     8341 tgttgcgcgc cgagggattc accgccgcca cgggtctgcg cgcgcacggt cagcggtact
     8401 tcgtactggc cgccggtcag gtagcggagc ttggtcgcct ggttgaccag cgggtccatc
     8461 gcggtgaaca ggaacgccgc cctggccagg tcgaccaccg gccgcagccc ggcggcggcg
     8521 agcccgacgg cgaagtccag ggtggcgttc tcggcgatcg gcatgctgcg gatgcgctcc
     8581 ggtccgaact cgtcgacgaa caggccgggc tgccaggtgc agaggtagac caggtccggg
     8641 tcgcggcgca gggcctggac gagggcggcg tgcagggcga tcatcggctc accacctggc
     8701 cacggtgagg tcgtcgcaga ccgtgccggg cgacggctcg ggctgggtga gcgcgtgcgc
     8761 ggcggccagg cgcatctcct cggcggcccg ccgccgcggt tcggtcaggt cgaagtccgc
     8821 gcgggacaac ggatcccggg cgcgccactg ccggacttcg tcgagcgggc ggtattcacc
     8881 gggtggctcg gggccgaact ggcagtggaa gtcgcgcagg taggtggagg cgtcgaggaa
     8941 ccgggcggtg cgctcgtcgc gaacgagggg gagcaactcg gcggcggtgc cggcgaccgc
     9001 tctgacgtcg ttcccgtcga cactcaccga cggcacgccg agcccggcgg cgacggcacc
     9061 gggcgcgatg ccggacacca gttcgctgcg ggtgcggtcc tggtatccgt tgttctcgca
     9121 gacgaacagc agcggcaggc gctggtgggc ggcgatccgc agggtctcca gcaccactcc
     9181 ggcaccgacc gcgccgtccc cgaagaacac caccaccgcg cccggctcct tccggatccg
     9241 cagcgcggcg gccattccgg cggcgatgcc gacgttggcg ccgacgatgg agttcccgcc
     9301 gaggaaccgg tgcgagcggt cggccaggtg catcgagccg cccttgccct tgttgagccc
     9361 ggtggcgcgg ccgagcagtt cggcgaacat cgcggccggg tccagcccgc acgccagcgc
     9421 gtgcccgtgg cagcggtagt agctgaccac gtggtctgcc gggccgagcc gcgcgcagac
     9481 cccggcggcg atcgcctcgt gcccggtggc ggggtggagc gcgccgggga ccttcccgcg
     9541 ccgctgcagt tccagcgcgg tctcctcgaa cgcgcggatc cggagcatgg tctcgtacaa
     9601 cggcagatcg cggcgctgga ctgcggtcgc ggcatccgcc agcgtcatct ggacctccgg
     9661 gaggtggggg agaaagccca ccatcgcaag gcggcccgcg gcacgtcatc gtgacaaggt
     9721 gagaagcgcc gctgatcccg ttgtcatgtg tgacaaccgc ggtgcgcggc gcgggccacc
     9781 gcgctggtca ggatgcgtac tccgcgcacg gagccggggt cgacgccgac gagttcgcgg
     9841 acccgccgca gccggtagtc cagcgtccgc ggatggatgt gcagtgcgag cgcggcgccg
     9901 agccggttca tgtcgttgcg gtagtaggca tccagcgtcg cgaccagttc cgggccgccc
     9961 gccagccgcc ggaccagttc gcccagccag tggtcgatct ccggtacccc ggccacgccc
    10021 agctcgacga agacctccgg aatgccgtag agcgtgctgg gcacccgttc acgtggcgcg
    10081 acgtcggcga cgcgcctggc cacttcggcg gcctcggcga gggctcccgc gtgcccggtc
    10141 gcggcaccca ccgcgcacgg caccccggcc gccgccacca tctccctgac caccgccagc
    10201 gcccggtcgt ggtccaccgg gagcagggcg accagctcgt ccggttcgtg ccaggtcgtc
    10261 accaccgaat gtcgcctgaa cgcggcttcg atgatttcgt cggcggcgcc gatcgatttc
    10321 gccgcgaaac gcagtaccgc gacaaaatac tcggcgtgcg cttcgacacc gagccgccgg
    10381 gcgatttcgg ccgtggtggt ggcattggcc agtagtgatt cggcgagttc gcggacccgt
    10441 tgcgccggtt tcacccgcaa cgcctgctcg tccagataac cgttgagata ggcggcgcaa
    10501 ccacggagcc cctgcgcgcc gaaccacgcg gtcaaccgga gcagttcggt cacgtccgcg
    10561 ctttcgcccg ccgccgcctg gatctcactg agcatcaggt tggtgtgcag ggtcagcgcg
    10621 gattccacgg acctggtggt gaacccgccc gccgcccgcg accggccgat ggcgccgatg
    10681 aacgaaagcg cgctttcacc cagtggttcg ccggtccgcg ccatttccac ggtgtgtctt
    10741 cgaaaccaca cggcgtagtc gagcaactcc gctttgccgt gcggattcgc cgcttcccgc
    10801 cggtaatccg ggatttccct gatgaaagtc tccaccgcgg cgcgcgcatt cgcttccgcg
    10861 cgtttcgcca gcaccccgaa aagatcactc accccgaact cccctcgatg tggaaagggt
    10921 agaggttgtg ggccgctggt caatccgcgt gcggcggcgg gcaagtccgc accggtgttc
    10981 ctgccaccgt gtcgaaacgt cgaagccgaa ccccggtggt gcgagcagga ggaaccccga
    11041 tgccaccttc cggcacttcg ccggccgggc cgccatcgac ccggcgctgc gggtgcgcgg
    11101 cgtggccggg tggcggatgc ctcgatcatg ccgaggtgcc ctcgtgcaac acgaacgcac
    11161 ccgccatcat ggtgggggag aaggcagcgg acctgatcac cggttgggga gggcggatgg
    11221 acgatccgca tatccacgtc gagcggaagg tgctgcactc cggtgccgac taccgcaacg
    11281 ccgtcgcgtc gatgctgggc gcggtgacgg acgccccggc cgtggtcacc accgggtgcg
    11341 gcatcgaggt gccgtacgcg atgacctcgc cgcacccgga gagcgtgacc tgcctggcct
    11401 gccgggaaca cgcggcccgt gagcaccggg cgttcgctgc gcaggtggag aagctgagcc
    11461 ggatgccggg ctcggtcgtg gccccggaag acgccgccaa ggccgtcgag cggcaccagg
    11521 acctggcccg gcggttcggc tgagcggtgc cgggggccgc cacccgtcgt gtcctactaa
    11581 gatgcttagt ggtggtaggc gtgcgctacc gcatgtccct tgccgcggcc gatcagccac
    11641 ttgttcaccg gcatggtgac caggaaggcg atcaggaagg cgaaggccag tgcgccccag
    11701 aacagccagg tgcccagtcc ggcgtccatg gctccgggga ccaccagcat cacgccgttg
    11761 tcgatgatct ccatcacggt gatcgacacg gtgtccgcgg ccagtgcgac cttcagcgcc
    11821 tgcttga
//
