LOCUS       NZ_CP045480            10393 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Amycolatopsis sp. YIM 10 chromosome, complete genome.
ACCESSION   NZ_CP045480
VERSION     NZ_CP045480.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. YIM 10
  ORGANISM  Amycolatopsis sp. YIM 10
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Complete genome sequence of Amycolatopsis sp. YIM 10, isolated from
            a rare earth mine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10312959)
  AUTHORS   Ruckert,C., Guerrero-Garzon,J.F., Zehl,M., Rezaei,S., Selivanova,G.,
            Urban,E., Busche,T., Kalinowski,J., Cao,Y.-R., Jiang,Y., Jiang,C.-L.
            and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2019) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009429145.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 04:03:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,775
            CDSs (total)                      :: 9,711
            Genes (coding)                    :: 9,634
            CDSs (with protein)               :: 9,634
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 22 of 77
            Pseudo Genes (incomplete)         :: 65 of 77
            Pseudo Genes (internal stop)      :: 1 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:01:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 621741
            Orig. end                         :: 632134
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045480.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(245..259)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.76"
                     /tool="antismash"
     misc_feature    250..265
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.09"
                     /tool="antismash"
     gene            293..1069
                     /locus_tag="YIM_RS03120"
                     /old_locus_tag="YIM_03135"
     CDS             293..1069
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985335.1"
                     /locus_tag="YIM_RS03120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03135"
                     /product="Abi-alpha family protein"
                     /protein_id="WP_228004525.1"
                     /transl_table=11
                     /translation="MGVPENFAERAGKLASIAARAGYTFGKRLPGADAAERGLRSLERL
                     ALTELRRRLEEVDDPYLAALSAASAMNVGNGRHIQPPLAGEIRGTVVVMPSSETIEPLR
                     AAMAELLNRSIGFGRERAREYLYAIILRQLTPDEARILSALADGAPFPLIDVTERNGLG
                     GAGRVVLRNASTVGKAAGVTLLDHVPSYVTRLIGLGLAELDEEVPELETQYEILLTDDA
                     VRAAENSVKRVKFVRRTVHISRLGKDFWAACDPARS"
     gene            1107..2342
                     /locus_tag="YIM_RS03125"
                     /old_locus_tag="YIM_03140"
     CDS             1107..2342
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631931.1"
                     /locus_tag="YIM_RS03125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03140"
                     /product="DUF445 domain-containing protein"
                     /protein_id="WP_153028892.1"
                     /transl_table=11
                     /translation="MSGIVDDFARNWPLYCSIPLIAALIGYTTKLVAIRMMFQPVEFVG
                     IKPFLGWQGVVPKRAARMAGIACDTMTQQLIKPSDVVNRLDPARIAKEIEKPLLAGVEE
                     IVREVAAEYQPGLWESLPTRVQRLVITRVQAESPRMVASIMDGVKQDVDSVFDLKDMVV
                     TSLVKDKRLLNRIFQEAGDKEFKFIARSGIFFGGAIGVVQMVVWMLFKLPVIMPLFGLF
                     TGWFTDWLALKMIFRPQQPKRYLGLFEWQGLFLKRRAEVSEAYGALIAKEIITPHNVIE
                     AVLRGPASDKVLALVQRQVDTELAKHTSLARPLVVMAVGSRRYQSMKLRISELIMARLP
                     ETMTYIEDYAEDAMDIRNLLVSKMKELPPDEFEGLLRPAFQQDEWILIATGALLGFAVG
                     EAQVLLLEHFAA"
     gene            2355..2891
                     /locus_tag="YIM_RS03130"
                     /old_locus_tag="YIM_03145"
     CDS             2355..2891
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638401.1"
                     /locus_tag="YIM_RS03130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03145"
                     /product="DinB family protein"
                     /protein_id="WP_153028893.1"
                     /transl_table=11
                     /translation="MSNSSSGEMAESVDRSWPETRAGDELTLLWGFLDFLRATAVNKVA
                     GLTRAEAAGCPFPASPEMNALGIIRHLTAVERWWLTIVGGGSDLPDLWGDTSDHDHLFR
                     LGKEQSPAELVAAYQEEWRISRDALAGMSADDAVRRPGEDKTVRWVLTHLVQETARHVG
                     HLDVLREFADGEVGE"
     misc_feature    2358..2373
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.59"
                     /tool="antismash"
     misc_feature    2961..2981
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.77"
                     /tool="antismash"
     misc_feature    complement(3056..3069)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     gene            3172..3672
                     /gene="ectA"
                     /locus_tag="YIM_RS03135"
                     /old_locus_tag="YIM_03150"
     CDS             3172..3672
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="YIM_RS03135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03150"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_153028894.1"
                     /transl_table=11
                     /translation="MSGKHLIESPTKADGAALWRIARDSQKLDLNSSYAYLLWCRDFAD
                     TSVVARSDGAAVGFVIGYRRPSDPEAALVWQVAVDASQRGKGLAGALLDALFDRLVGQG
                     VRYLETTITPDNQASIKLFESFAKRWEADLESAELFGSADFPDDDGVHEPEHLYRIGPL
                     TRR"
     gene            3727..4980
                     /gene="ectB"
                     /locus_tag="YIM_RS03140"
                     /old_locus_tag="YIM_03155"
     CDS             3727..4980
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 4.5e-73.
                     Score: 238.1. Matches aSDomain:
                     nrpspksdomains_YIM_RS03140_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="YIM_RS03140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03155"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_153036752.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4e-73, bitscore:
                     245.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIFSKLESEVRSYSRGWPVVFDRAQGSMLYDETGKGYLDFFAGA
                     GALNYGHNNPALKQALIDYIARDGVTHALDMFTVAKRDFLETLQEKILGPRELDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDRYFDGAYPDFLYFERLLEDSGSGLNEPAAVIVEALQGEGGINAARLEWLKGLS
                     DLCERHNILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGLPLALTLIKPE
                     LDVWEPGEHNGTFRGINPAFVTATEALRVYWSDDKLEQSTRAKGERIGSVFDEIVQAYP
                     EANLVAKGRGLARGIEFATGELASSVCADAFERGLLMETSGPDGEVMKLLPALTINDAE
                     LEQGLEIIGESIRAVLTK"
     misc_feature    complement(3732..3746)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.86"
                     /tool="antismash"
     aSDomain        3790..4800
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_YIM_RS03140_Aminotran_3.1"
                     /evalue="4.50E-73"
                     /label="YIM_RS03140_Aminotran_3.1"
                     /locus_tag="YIM_RS03140"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.1"
                     /tool="antismash"
                     /translation="FDRAQGSMLYDETGKGYLDFFAGAGALNYGHNNPALKQALIDYIA
                     RDGVTHALDMFTVAKRDFLETLQEKILGPRELDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDRYFDGAYPDFLYFER
                     LLEDSGSGLNEPAAVIVEALQGEGGINAARLEWLKGLSDLCERHNILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGLPLALTLIKPELDVWEPGEHNGTFRGINPAFV
                     TATEALRVYWSDDKLEQSTRAKGERIGSVFDEIVQAYPEANLVAKGRGLARGIEFA"
     gene            5001..5393
                     /locus_tag="YIM_RS03145"
                     /old_locus_tag="YIM_03160"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084077.1"
                     /locus_tag="YIM_RS03145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03160"
                     /product="ectoine synthase"
                     /protein_id="WP_113694800.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.9e-53, bitscore:
                     178.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDADIKTPNWRSKRIILAKEKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVYEGEGELVNKATGERHQLKPGSLYLLNDHDKHQVLPKTDMKTV
                     CVFNPPVTGREVHDENGVYPLVTEES"
     gene            5438..6334
                     /gene="thpD"
                     /locus_tag="YIM_RS03150"
                     /old_locus_tag="YIM_03165"
     CDS             5438..6334
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669885.1"
                     /locus_tag="YIM_RS03150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03165"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_370468950.1"
                     /sec_met_domain="PhyH (E-value: 7.5e-36, bitscore: 123.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTRIGDAYPTRLPAGEAGAIDRLDPTVWGNEADGPIDAATLASH
                     DTKGYSVVEGLLSPAEVQAYWQELVRLSGDQELKADERVVTEKTSGEVRSIFEVHKISE
                     LIAELVRDPRVLDRARQILGSDVYVHQSRVNYMPGFRGNGFYWHSDFETWHAEDGMPVP
                     RAVSCSIALTDNYPFNGGLMLMPGSQRTFVQCAGRTPENNYKSSLKEQKVGVPAEDEIA
                     KLAAEHGIEQFTGAAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVDNAVVEPFAAG
                     KARPEFIAARDFTPVRR"
     gene            6600..9395
                     /locus_tag="YIM_RS03155"
                     /old_locus_tag="YIM_03170"
     CDS             6600..9395
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664381.1"
                     /locus_tag="YIM_RS03155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YIM_03170"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_228004526.1"
                     /transl_table=11
                     /translation="MSEGDFASRQAWFQLLGPVQLFAGSRPVPVGGPGVRGLLALLALH
                     ANKVVPLDDIIDALWGHDPPATARTIVHGNVSHLRRVLRSIQGDGGDGAQIHTRPPGYQ
                     LAVDPSQIDVHRARALLEYATSAGAERRAGVLAEALALWKGPVLSGVPDSVHAPELEDL
                     RLAVHGARVDADLELGRHAELISELTTMVRENPLAERTIGQLMRALYHAGRRADALAVY
                     RRAARHVVGTLGIDPGAELRELHDRILRDDLADPSAPKGPPPRLVRVNPAQLPPAVPRF
                     AGRVEELAWLDALVEQARHGASLVGVVTGAPGVGKSALAISWAHHTAGDFPDGVLFATL
                     RGFDPEHSPLAPGEVVTQFLLGLGVRPAELPDKPHERIALYRSLLADATVLVVLDDARS
                     AEQVRPLLPPAPGSVAVVTSRARLDGLVVSSAARIKALETMPPADAVRLVEELAGPGDY
                     GKLAELCAYLPLALRIAGARLVVSPQWTVDDLVAELADERTRLTALDVAGVDVAETSVR
                     AALDVSHRGLPEPVGALFRLLGAFTGPSIGAHLAAALGGTDVAEARRLLRTLAAYHLLT
                     EVGRDVFAPHDLIRLYLRGLAETELAATEREAVLRRSVRYYQAAADRARRRLLRIVDSL
                     DFAAVDTELPVLAGFDDALDWFALEWPNLLETCEAAYRAGRYEDAWRLARVVHTYRVVR
                     PLRDEWTGLVELGLSAAKASGNPLAHCWMLISRCAIALTFGIEGGGLADAEHALELAAE
                     VGDKRLLVSANIHVGAALSSSGRYDEAIERLRHAIEETERTGDLALRGQALNNCAEAEK
                     LSGRYAEAIEHQLGSLAIDRELGDDSYAVVSLNNLAELYLETGELGEAERYARQAIGLT
                     TSRGFLLQEGVSRAALGRVLVARGDRVQAREQLELAEERFRQAGAPWPAELGAELAALD
                     E"
ORIGIN
        1 cccggtcccc gacccgcccg cgaaggaact gcgcggcacc cgcgtcttcc tgtccaacgg
       61 cgagcgcgac ccgatggcgc cgctggcctc gaccgagcag tacgtggagt tcctgcggga
      121 acgctcggcc gaggtcaccg agcaccggca cgccggtggc caccagatca ccctcgaagg
      181 gctggccgcc gcgaaggcct ggctcgtggt gtgatggaag caacggcgtc cgttcggcct
      241 actgaaccta ctgaaaagta gtatccgggg cgtcacgagg ggagggcgcg ccgtgggtgt
      301 tccggagaac ttcgccgagc gggcgggcaa gctggcgagc atcgccgccc gcgcggggta
      361 cacgttcggc aagcgcctgc ccggcgccga cgccgccgag cgcgggctcc gctcgctcga
      421 acggctcgcg ctgaccgagc tgcgacggcg actcgaagag gtcgacgacc cgtacctcgc
      481 cgcgctgagc gccgcgtcgg cgatgaacgt cggcaacggg cggcacatcc agcccccact
      541 ggccggcgag atccgcggca cggtggtggt gatgccgtcc tcggagacca tcgaaccgct
      601 gcgcgcggcg atggccgagc tgctgaaccg gtccatcggc ttcggccgcg agcgcgcccg
      661 cgagtacctc tacgcgatca tcctgcgcca gctcaccccg gacgaggcac gcatcctgtc
      721 cgcgctggcc gacggtgcgc ccttcccgct gatcgacgtc accgagcgca acggcctcgg
      781 tggtgccggg cgggtggtgc tgcgcaacgc gtccactgtg ggcaaagccg ccggggtgac
      841 cctgctcgac cacgtgccca gctacgtcac ccggctgatc gggctcggcc tggccgaact
      901 ggacgaggaa gtgccggaac tggagaccca gtacgagatc ctgctgaccg acgacgcggt
      961 gcgggccgcg gagaactcgg tcaaacgggt gaagttcgtt cgccggaccg tgcacatctc
     1021 gcggctcggc aaggatttct gggcggcctg cgacccggcg cgaagctgat cattgtccca
     1081 ggtggacccc cgtgtgatca tgagccatga gcggcatcgt cgatgacttc gcgcggaact
     1141 ggccgctcta ctgctccatt ccgctgatcg ccgcgctgat cgggtacacc accaagctgg
     1201 tggccatccg gatgatgttc cagccggtgg aattcgttgg catcaaaccg ttcctgggct
     1261 ggcagggggt ggtgcccaag cgtgcggcgc ggatggcggg catcgcctgc gacacgatga
     1321 cccagcagct gatcaagccg agcgacgtgg tcaaccggct cgaccccgcg cgcatcgcca
     1381 aggagatcga gaagccgctg ctggccggcg tcgaggagat cgtgcgtgag gtggccgccg
     1441 agtaccagcc ggggctgtgg gaatcgctgc cgacgcgggt gcagcggctg gtgatcaccc
     1501 gcgtgcaggc cgagtcgccg cgcatggtcg cgtcgatcat ggacggggtc aaacaggacg
     1561 tggacagcgt cttcgacctc aaggacatgg tggtgaccag cctggtcaag gacaaacggc
     1621 tgctcaaccg gatcttccag gaggcgggcg acaaggagtt caagttcatc gcccgctccg
     1681 gaatcttctt cggcggggcc atcggcgtgg tccagatggt ggtctggatg ctgttcaagc
     1741 tcccggtgat catgccgctg ttcggcctgt tcaccgggtg gttcaccgac tggctggcgc
     1801 tgaagatgat cttccggccg cagcagccga agcgctacct cggcctgttc gagtggcagg
     1861 gcctgttcct caagcgccgc gccgaggtgt ccgaggccta cggcgcgctg atcgccaagg
     1921 agatcatcac cccgcacaac gtgatcgagg cggtgctgcg cgggcccgcc tcggacaagg
     1981 tgctggcgct ggtccagcgg caggtggaca cggagctggc caagcacacc agcctggcca
     2041 ggccgctggt ggtgatggcg gtgggcagca ggcgctacca gtcgatgaag ctgcggatct
     2101 ccgagctgat catggcgcgg ctgccggaga cgatgaccta catcgaggac tacgccgaag
     2161 acgcgatgga catccggaac ctgctggtca gcaagatgaa ggagctgccg cccgacgagt
     2221 tcgagggcct gctccggccc gccttccagc aggacgaatg gatcctgatc gccaccggcg
     2281 cgctgctcgg cttcgccgtg ggtgaggcgc aggtcctgct gctggagcac ttcgccgcct
     2341 gatacatttt ccgcatgtcg aactccagtt cgggagaaat ggcggaatcg gtcgaccggt
     2401 cctggccgga gacccgggcc ggcgacgagc tgaccctgct ctggggcttc ctcgacttcc
     2461 tgcgcgcgac ggcggtcaac aaggtcgccg ggctgaccag ggccgaggcc gcgggttgcc
     2521 cgttcccggc atcaccggag atgaacgcgc tcgggatcat ccggcacctg accgcggtcg
     2581 agcggtggtg gctgaccatc gtcggcggcg gcagcgacct gcccgacctg tggggcgaca
     2641 ccagcgatca cgaccacctg ttccgcctcg gcaaggagca gtcgcccgcc gagctggtgg
     2701 ccgcctacca ggaggaatgg cggatctcgc gtgatgcgct ggccgggatg tccgccgatg
     2761 acgcggtgcg acgacctggc gaggacaaga ccgttcggtg ggtgctcacc cacctggtcc
     2821 aggagaccgc gaggcacgtc gggcacctcg acgtactgcg tgaattcgcc gacggcgagg
     2881 tcggggagta agcccctccg gctgggtaaa gtaccggggt acccagcgta tgcttaggcc
     2941 aaacgtgcac tttgggtcgc atctcgtgag cgtgaccact accgtgtccg aagtgagacc
     3001 gcttcgtcac gaagcggtat cggtgggctg cggcattata ggccacggaa agcagcaaac
     3061 ctacgttact gttgccatac tcacagggtt ttctgtgtct gcgctggtag gccacctgca
     3121 ctagttcgtg cgggctgagg cccctttgcg attctgttaa gttcgtttga catgtccgga
     3181 aagcacttga tcgaatcccc gaccaaggca gatggtgccg cgctctggcg tatcgcgcgc
     3241 gattcccaga agctcgatct gaactcgtcg tacgcctatc tgctgtggtg ccgcgatttc
     3301 gccgacacct cggtggtcgc gcgatccgac ggcgcggcgg tcggcttcgt gatcggctac
     3361 cgcaggccgt ccgacccgga ggcggcgctg gtctggcagg tcgccgtcga cgcgtcgcag
     3421 cggggaaaag ggctggctgg agcgttgctc gacgccctgt tcgaccggct ggtcggccag
     3481 ggcgtccgct acctcgagac cacgatcacc ccggacaacc aagcttccat caagctcttc
     3541 gagtccttcg cgaagcgctg ggaagccgat ctggaaagcg ctgaactctt cggttccgcc
     3601 gatttcccgg atgacgacgg cgtgcacgaa cccgagcacc tttatcgaat cgggccgttg
     3661 acgcgcagat aattcgcaca tggccacggg gttactccac agggacggac ggaactggcg
     3721 atgaacatgg aaattttctc gaaactcgaa tccgaggtgc gcagttacag ccgcggctgg
     3781 ccggtggtct tcgaccgggc gcagggcagc atgctctacg acgagaccgg caagggctat
     3841 ctggacttct tcgccggcgc gggcgcactg aactacggcc acaacaaccc ggcgctcaag
     3901 caggcgctga tcgactacat cgcgcgcgac ggcgtgaccc acgcgctgga catgttcacc
     3961 gtggccaagc gcgacttcct ggagacgctg caggagaaga tcctcggtcc gcgcgagctg
     4021 gactacaagg tcgtcttccc cggtcccggt ggcgcgaacg ccgtcgaagc cgcgctcaag
     4081 ctggcccgca aggtgaccgg caaggagtcg gtcatcaact tcaccaacgc cttccacggc
     4141 atgacgctgg gcgcgctgtc ggtcaccggc aactcgatga agcgcggcgg cgccggtgtg
     4201 ccgctggtgc acgccacccc gatgccgtac gaccgctact tcgacggcgc ctacccggac
     4261 ttcctgtact tcgagcggtt gctcgaggac tccggcagcg ggctgaacga gccggccgcg
     4321 gtgatcgtcg aggcgctgca gggcgagggc ggcatcaacg cggccaggct ggagtggctc
     4381 aagggcctgt ccgacctgtg cgagcggcac aacatcctgc tcatcctcga cgacgtgcag
     4441 atgggctgcg gccgcaccgg cccgttcttc agcttcgagg acgccggcat caaaccggac
     4501 atcgtctgcc tgtcgaagtc gatcggcggt tacggcctgc cgctcgcgct gacgctgatc
     4561 aagccggagc tggacgtgtg ggagccgggt gagcacaacg gcaccttccg cggcatcaac
     4621 ccggcgttcg tcaccgccac cgaagcgctg cgcgtgtact ggagcgacga caagctcgag
     4681 cagtccaccc gcgccaaggg cgagcgcatc ggctcggtct tcgacgagat cgtgcaggcc
     4741 tatcccgaag cgaacctggt ggccaagggc cgcggcctgg cccgcggcat cgagttcgcc
     4801 accggcgagc tggcgagttc ggtgtgcgcg gatgccttcg agcgtggtct gctgatggag
     4861 acctccgggc ccgacggtga ggtgatgaag ctgctgcccg cgctgaccat caacgacgcc
     4921 gagctggagc agggcctgga gatcatcggc gagtccatcc gcgccgtgct gaccaagtag
     4981 gaagctaagg agacacgact ttgatcgtcc gcaccctcga cgagatcacc gacaccgacg
     5041 ccgacatcaa gacgccgaac tggcgcagca agcgcatcat cctggcgaag gagaaggtcg
     5101 gcttctcggt gcacgagacc acgctctacg cgggcacggt caacgacttc tggtacgcca
     5161 accacatcga ggccgtgttt gtctacgagg gcgagggcga gctggtcaac aaggcgaccg
     5221 gtgagcgcca ccagctcaag cccggttcgc tgtacctgct caacgaccac gacaagcacc
     5281 aggtgctgcc caagaccgac atgaagacgg tctgcgtctt caatccgccg gtgaccggcc
     5341 gcgaggtcca cgacgagaac ggcgtgtacc cgctggtgac cgaagaatcc tgatcggaaa
     5401 gagcgcgatt cacgaggagg cgagcagttt gactctgatg gacacccgga tcggggacgc
     5461 ctacccgacc cgactcccgg cgggggaggc cggggcgatc gaccgccttg atcccacggt
     5521 gtggggcaac gaagcggacg gtccgatcga tgcggccacc ctggcctcgc acgacaccaa
     5581 gggctactcg gtggtggagg gcctgctctc cccggccgag gtgcaggcct actggcagga
     5641 actcgtccgg ctctccggtg accaggagct gaaggcggac gagcgggtgg tgaccgagaa
     5701 gaccagtggc gaggtccgct cgatcttcga ggtgcacaag atcagcgaac tgatcgccga
     5761 gctggtccgg gatccgcgcg tgctcgaccg ggcgcgccag atcctcggat ccgacgtgta
     5821 cgtccatcag agccgggtca actacatgcc cgggtttcgc ggcaacggct tctactggca
     5881 ctccgacttc gagacctggc acgccgagga cggcatgccg gtgccgaggg cggtcagctg
     5941 ctccatcgcg ctcaccgaca actacccgtt caacggcggg ctgatgctga tgccgggctc
     6001 ccagcgcacg ttcgtgcagt gcgccgggcg gaccccggag aacaactaca agtcctcgct
     6061 caaggagcag aaggtgggcg tgcccgccga ggacgagatc gccaagctcg ccgccgagca
     6121 cgggatcgag cagttcaccg gcgccgcggg ttcggcgctg tggttcgact cgaacatcat
     6181 gcacggctcc gggaacaaca tcaccccgta cccgcgctcg aacatcttcc tggtgttcaa
     6241 cagcgtggac aacgcggtgg tggagccgtt cgcggcgggc aaggcgaggc cggagttcat
     6301 cgccgcccgc gacttcaccc cggtccggcg ataatcgtta tcaggccgcg aggggtagct
     6361 cttcgctaac ccttcgtggc ctgttacgtt tgtctccgtc acggtcgcag tgacgcgacg
     6421 gttactcctt gctcggggtg agatcgtccg ctcgcgggcc gtggcagtcg gcaacaccgc
     6481 cctgcaccat cagatcggga ctgatggtgc agggcggtgt tgtttccggc gtgccccgct
     6541 tctcgcccgg tttgcgagta acgtcttcac ctgctcgtcc acgacgcagg ggaggcgtag
     6601 tgagcgaagg ggattttgcg tcccggcaag cctggttcca gctgctcggc ccggtgcaac
     6661 tgttcgcggg ctcgcgcccg gttccggtcg gcgggccggg cgtgcgcgga ctgctggcgc
     6721 tgctggcctt gcacgccaac aaagtcgttc cactggacga catcatcgac gcgttgtggg
     6781 ggcatgatcc accggccacc gcgcggacca tcgtgcacgg caacgtgtcc cacctccgcc
     6841 gcgtgctgcg gtccatccag ggcgacggcg gggacggcgc gcagatccac acccggccgc
     6901 ccggttacca gctggccgtc gacccgtcgc agatcgacgt gcaccgcgcg cgggccctgc
     6961 tcgagtacgc cacttcggcc ggtgccgagc gccgcgccgg cgtgctcgcc gaggcgctcg
     7021 cgctgtggaa gggcccggtg ctctccggtg tgcccgattc ggtgcacgcg cccgaattgg
     7081 aggacctccg gctcgcggtg cacggcgccc gcgtcgacgc cgacctcgaa ctcgggcggc
     7141 acgccgagct gatctccgag ctgaccacca tggtccgcga gaacccgttg gcggaacgca
     7201 cgatcggcca gctgatgcgg gcgctctacc acgccgggcg ccgcgccgac gcgctcgcgg
     7261 tgtaccgtcg cgcggcccgg cacgtggtcg gcacgctcgg catcgatccc ggcgccgaac
     7321 tgcgcgagct gcacgaccgc atcctgcgcg acgacctcgc cgacccgtcg gcgccgaagg
     7381 ggccaccgcc ccggctggtc cgggtgaacc ccgcccagct gccgcccgcg gtgccccggt
     7441 tcgccgggcg cgtggaggag ctggcctggc tggacgcgct ggtcgagcag gcgcggcacg
     7501 gtgcgagcct ggtcggcgtg gtcaccggtg cgcccggcgt cggcaagagc gcgctggcga
     7561 tctcgtgggc gcaccacacc gcgggggact ttcccgacgg tgtgctgttc gcgacgttgc
     7621 gcggcttcga cccggagcac tcgccgctcg cgcccggtga agtggtcacc cagttcctgc
     7681 tcgggctcgg cgtccggccc gccgaactcc cggacaaacc acacgagcgc atcgcgctgt
     7741 accgctcgct gctggccgac gcgacggtgc tggtggtgct cgacgacgcg cgctcggccg
     7801 agcaggtccg cccgctgctg ccgcccgcac cgggctcggt cgccgtggtg accagccgcg
     7861 cgcggctgga cgggctggtg gtgtccagcg cggcgcgcat caaggcgctg gagaccatgc
     7921 cgcccgccga cgcggtgcga ctggtcgagg agctggccgg gccgggtgac tacggcaagc
     7981 tggccgagtt gtgcgcgtac ctgccgctgg cgctgcgcat cgccggtgcc cggctggtgg
     8041 tgagtccaca gtggaccgtc gacgacctgg tggcggagct ggccgacgag cggacccggc
     8101 tgaccgcgct ggacgtggcc ggggtggatg tggcggagac cagcgtgcgg gccgcgctgg
     8161 acgtgtccca ccgggggctg ccggagccgg tcggcgcgtt gttccggttg ctcggcgcgt
     8221 tcaccgggcc gtcgatcggg gcgcacctgg ccgccgcgct cggcggcacc gacgtcgccg
     8281 aggcgcgccg actgttgcgc acgctcgcgg cgtaccacct gctcaccgag gtcggccggg
     8341 acgtgttcgc gccgcacgac ctgatccggc tgtacctgcg cggtctcgcc gagacggaac
     8401 tggccgccac cgaacgcgag gcggtgctgc ggcggtccgt gcggtactac caggcggcgg
     8461 ccgaccgggc gcgacggcgg ctgctgcgca tcgtcgactc gctcgacttc gccgcggtgg
     8521 acacggaact tcccgtgctc gccggcttcg acgacgcgct ggactggttc gcgctggaat
     8581 ggccgaacct gctcgaaacc tgcgaagccg cctaccgggc cgggcggtac gaggacgcct
     8641 ggcggctcgc gcgcgtggtg cacacctatc gcgtggtgcg gccgttgcgg gacgagtgga
     8701 ccgggctggt cgagctgggc ctgtccgcgg cgaaggcgtc cgggaacccg ctcgcgcact
     8761 gctggatgct catctcgcgg tgcgccatcg cgctcacctt cggcatcgag ggcggtgggc
     8821 tcgccgacgc cgagcacgcg ctcgaactgg ccgccgaggt cggggacaag cgcctgctgg
     8881 tgtcggcgaa catccacgtg ggcgcggcgc tgagcagctc cggccggtac gacgaggcga
     8941 tcgagcggct gcggcacgcg atcgaggaga ccgagcggac cggcgatctc gcgttgcgcg
     9001 ggcaggcgct gaacaactgc gccgaggcgg agaagctgtc cgggcggtac gccgaggcga
     9061 tcgagcacca gctcggctcg ctggcgatcg atcgtgaact cggggacgac agttacgctg
     9121 tggtatcgct gaacaacctg gcggagctgt acctggaaac cggggaattg ggcgaggcgg
     9181 aacgctatgc gcggcaggca atagggctga ccactagtcg tggattcctg ttgcaggagg
     9241 gcgtttcccg ggcggcgctg ggccgggtgc tggtggctcg cggtgaccgg gtgcaggccc
     9301 gtgagcagct cgaactcgcc gaagaacggt tccgccaggc gggcgcgccg tggccggcgg
     9361 agcttggtgc ggaactcgcc gcattggacg aatagcggta agtcaccacc gaagtccatc
     9421 gagcggaaga tcctcgcgga caggtgaaat atctacttag tgaactctta gcagggtggc
     9481 ggcgctcacg cgtagtgttc aaaacgcggt gatcgttcgg cggccgggga gggcctgaag
     9541 ccggcgatca ccgcggcttc cgggtaccgc ggtgggacgc gagcggaagc caccgcgccg
     9601 gaccgggcca gggggagggt ccggcgcacc accggccggt cggccggcgc gccgaggggg
     9661 agcgccggcc gatcgcccgc tgatggtcca gcccatgtgg ctttcacggc actgctttca
     9721 cggcgctggt cgggggccac cccgctgttt gatttcgccg tgtttttggc tgtggagcgg
     9781 ggatggggag gggctgggtg gggtatgggg tcggctgtcg ttgccgggtg gcggttctgg
     9841 gggttggttt ggggtccggc gcctggcgga ttctctagaa gaggctggtt tccggggtga
     9901 tctcgagtag ttcgtagacc gggcggcccc ggcggccgtc gatggtggtg gtgcggacga
     9961 cgcgcaggcg cgcggtcacc ggccggtcca ggttcagttt gatctcgtcc agcaggtccg
    10021 gcgccaccgc gccctgcacc gtgccgcccg actcgcgctc caggtagaag atccgccgcc
    10081 gggtgcgcac accgtcgagc ctgccggaaa ccgtctcgta cccgacctcc tcgcgcgact
    10141 cccgcaggct gccccgcagc acgcgcgcct gctcggtggt catgctgcgc agcacgtcct
    10201 caccggccgt cgtggtcagc tgcaagccga tcccggcgtt cttgctgacc gccgccacga
    10261 tgtcgctgac cgcgttccgc acggtgtcgc gctgcagcag caccgcgtcc agcgcgccgt
    10321 cgtccgaacc gttggcgggc aggaagtcgc acagctcctt gaccgcgcgc tcggacaacg
    10381 tctcgatgcc gtc
//
