LOCUS       NZ_CP045178            10525 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8102128
            Orig. end                         :: 8112653
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10525
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="35"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10525
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10525
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5525](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5525
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(328..1329)
                     /locus_tag="GBW32_RS33420"
                     /old_locus_tag="GBW32_33415"
     CDS             complement(328..1329)
                     /NRPS_PKS="Domain: Abhydrolase_1 (59-170). E-value: 7e-17.
                     Score: 53.8. Matches aSDomain:
                     nrpspksdomains_GBW32_RS33420_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826208.1"
                     /locus_tag="GBW32_RS33420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33415"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_077974437.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.3e-12, bitscore:
                     47.5, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 2.3e-13, bitscore: 49.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPHSTAVVCGQSTSEAVRTVPGQERVAARVSSGPAEHEMTFDGFR
                     FVGRVSGRPEPGRDPVLILGGSSQDRNSWVRHERWLAPLGQVITVDLPGYGTADFLPAS
                     YGVDFLAAALRHLLDGLGVARVNMVAACYGGAVGLRFAQHYPAMVRRLMLVGMTTRIPS
                     DYAAAMKRWAAMIDRGETEPIAQELTERFMSPPGIGVVRKHTAVSRLVYRQIAGQTPEQ
                     LAMSAEHNTRLMLHEWYRPEPSPPVPSLVVTGEHDTLCTPDMGREVAAVLPAAQFLTIQ
                     ETDHLAPVERMEDFSDLVVRFCTDESLAGLPYTTALEHFGPGLPARPAPANG"
     aSDomain        complement(820..1152)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS33420_Abhydrolase_1.1"
                     /evalue="7.00E-17"
                     /label="GBW32_RS33420_Abhydrolase_1.1"
                     /locus_tag="GBW32_RS33420"
                     /protein_end="170"
                     /protein_start="59"
                     /score="53.8"
                     /tool="antismash"
                     /translation="PVLILGGSSQDRNSWVRHERWLAPLGQVITVDLPGYGTADFLPAS
                     YGVDFLAAALRHLLDGLGVARVNMVAACYGGAVGLRFAQHYPAMVRRLMLVGMTTRIPS
                     DYAAAMK"
     misc_feature    complement(1402..1420)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.42"
                     /tool="antismash"
     gene            1658..2614
                     /locus_tag="GBW32_RS33425"
                     /old_locus_tag="GBW32_33420"
     CDS             1658..2614
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849230.1"
                     /locus_tag="GBW32_RS33425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33420"
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_077974436.1"
                     /transl_table=11
                     /translation="MTVHPSRPETATAVADRGAPEALRRARTALRRTPVSIWNDDVSDW
                     AAALTYYSILALLPALLVTVSVIGLAAPGAVDSLIADITSYAPAEAGGALRASLEDMAH
                     EHSAVWLLIATGAVSALWSASSYLAVFRRALNTMHQVRDTRHPLRKAHTIVVTAVVLLV
                     LLLTSAAVLVVTGPLARGISPEIGVSGADAWTILKWPLLLCVVTVLIFILFRSGPPAAR
                     GVRRGLPGGILAALLWLAASGGFALYATRFGSYSRLYGSLAGVVVFLVWLWFTNLSLLV
                     GAQFNVEMARLAPDAKPEGERAAPAGEADGPTAAGRP"
     gene            complement(2707..4497)
                     /locus_tag="GBW32_RS33430"
                     /old_locus_tag="GBW32_33425"
     CDS             complement(2707..4497)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154253.1"
                     /locus_tag="GBW32_RS33430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33425"
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="WP_152330849.1"
                     /transl_table=11
                     /translation="MHVPPTDSSLFHRPPPSSARRRATVLSAVSALVMSFLVFAPAGAA
                     SADVPGGGDLGPNVKVFDPSTPDIQGQVDAIFKQQESAQFGQGRYQLLFKPGTYDNINA
                     QLGFYTSISGLGLSPDDTNINGDVTVDAGWFDGNATQNFWRSAENLALKPVSGTDRWAV
                     SQAAPFRRMHVKGGLNLAPDGYGWASGGYIADSKIDGSVGPYSQQQWYTRDSQVGGWGN
                     GVWNMVFSGVEGAPAQSFPEPPYTTLDTTPVSREKPFLYLDGDDYKVFVPAKRTDAHGT
                     SWGGGTPQGESIPLDKFYVVKPGATAETINAAVKQGLHLLFTPGVYHVDRTIDIDRADT
                     VVLGLGYATIVPDNGVTAMKVGDVDGVKLAGLLIDAGTQNSDTLLEVGPEDASADHSAN
                     PTTVQDVFVRIGGAGAGKATTSMVINNDDTIVDHTWIWRADHGDGVGWETNRADYGLRV
                     NGDDVLATGLFVEHFNKYDVEWHGERGRTIFFQNEKAYDAPNQAAVQNGDQKGYAAYKV
                     DDSVTTHEGWGLGSYCYYNVDPTIRQGHGFEVPEKPGVKFHDLLVVSLGGKGQYDHVIN
                     ETGSPTSGDTTVPSQVTSFP"
     misc_feature    complement(4641..4656)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.34"
                     /tool="antismash"
     gene            5001..5525
                     /locus_tag="GBW32_RS33435"
                     /old_locus_tag="GBW32_33430"
     CDS             5001..5525
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035840.1"
                     /locus_tag="GBW32_RS33435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33430"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_227025397.1"
                     /sec_met_domain="melC (E-value: 8e-42, bitscore: 141.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSRTPSGDLGPKDGPPDMAPRGETGKPDPATGSGPFSRRGALRA
                     LFTVSAVAGTGAALAPLLAAETSPAAPRAAGATVLAARGGRGGETLFDEVYRGRRIQGR
                     ALPRGAGGAKARIQATVDGRVLPLTRRADGGCLSPVDHYDSYATPLEATRAAVDELGGA
                     RLSPAAARHHH"
     gene            5570..6436
                     /locus_tag="GBW32_RS33440"
                     /old_locus_tag="GBW32_33435"
     CDS             5570..6436
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071025.1"
                     /locus_tag="GBW32_RS33440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33435"
                     /product="tyrosinase family protein"
                     /protein_id="WP_077973749.1"
                     /transl_table=11
                     /translation="MVYTRKNQRHLTKAEKRKFVAAVLELKRRGDYDAFVRTHVEYYVS
                     DGDDGLRVAHMAPSFLPWHRQFLLEFERALRKIDPSVTVPYWDWTKDRTPASSLWSEDF
                     LGGDGRRGDRQVTTGPFAHARGHWNIVEGVSEDPFLAREFGRPRDPIDLPTGEELARAT
                     AEGLYDVEPWDSTSDHGFRNKLEGWTRGRGNQRFRNHNRVHRWIGGNMLGGASVNDPVF
                     WLHHSFIDLVWSRWQRAHPDAGYLPAKPPPAGSAQYGRVAALEDPMPPWNVRPSALLGH
                     GHLYRYA"
     gene            complement(6648..6920)
                     /locus_tag="GBW32_RS33445"
                     /old_locus_tag="GBW32_33440"
     CDS             complement(6648..6920)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035838.1"
                     /locus_tag="GBW32_RS33445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33440"
                     /product="chaplin"
                     /protein_id="WP_077973748.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVIAGTGAVMAAGAGMATADAGAQAAAIGSPGVLSGN
                     AVQVPVHIPVNLCGNTVNVIGLLNPAFGNTCVNTTVHHHHGGYGG"
     gene            7130..7717
                     /locus_tag="GBW32_RS33450"
                     /old_locus_tag="GBW32_33445"
     CDS             7130..7717
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640371.1"
                     /locus_tag="GBW32_RS33450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33445"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_077973747.1"
                     /transl_table=11
                     /translation="MQHTSNRPAEETARRPPPAVSDPAHRSDVTLRVNGERHTVSLDHR
                     TTILDTLREELGLTGAKKGCDHGQCGACTVLVDGRRANSCLLLAVSVDGADITTVEGVT
                     DGEDLHPLQEAFLRRDALQCGYCTPGQICSALGVLGEAAAGHPSLVTPADAPHTGEPME
                     LDAREIRERMSGNLCRCGAYPRIVEAVQDVTR"
     gene            7714..8742
                     /locus_tag="GBW32_RS33455"
                     /old_locus_tag="GBW32_33450"
     CDS             7714..8742
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566463.1"
                     /locus_tag="GBW32_RS33455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_33450"
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="WP_077973746.1"
                     /transl_table=11
                     /translation="MKPFAYRRPRTATEAVDALAAHPGARFLGGGTNLVDLMKLGVETP
                     HTLVDVSGLPLDTVEELTDGSLRVGATVRNSDLAAHPAVRARYPVLSQALLAGASGQLR
                     NVATTGGNLLQRTRCPYFQDTSQPCNKRTPGSGCAAVEGHNREHAILGHSPRCVATNPS
                     DLAVALCVLDASVELLGPEGERTVAAADFHRLPGDHPERDSHIRPGELITAVVLPARPA
                     GLPSVYRKARDRTSYAFALVSVGAVLDVRDDGVIRYARLAFGGVAHRPWRALHAEDILC
                     GAHATEETFTRALDAELDQARPLRDNAFKVTLARRLATDVLTGLADPGAPLTPWPHTTE
                     QS"
     misc_feature    complement(9081..9095)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 agcaccacgg catcccgatc gggttcgcca accccggcat ctaccagcgc tacggcacgt
       61 cggcctatca cgacgtgacc gaccagccgc tgggcagggg ccagggcctg gccgtcgtcc
      121 gtaccgactt cgtcaacggt gtcgacgaca gcgaaggcac caccacgtcc ctgcggacac
      181 tcggcaagga cagctcgctc tccgccgtcg tgggctatga cgacgtgacg ggtgtgggct
      241 caccggccgg cggctacatc gactcctacc gcgaccactg atcccgacgc cgcccggcgt
      301 tgtgccgccg ggcggcaccg gtgacgctca gccgttggcc ggcgcgggcc gggccgggag
      361 gccggggccg aagtgctcca gtgcggtcgt gtacggcagc ccggccagcg actcgtcggt
      421 gcagaagcgc accacgaggt cggagaagtc ctccatgcgc tccacgggcg cgaggtgatc
      481 ggtctcctgg atggtgagga actgtgccgc gggaaggact gccgccacct ccctgcccat
      541 gtccggggtg cacagggtgt cgtgctcgcc ggtcaccacc aggctcggca ccggcggtga
      601 gggctcgggc ctgtaccact cgtgcagcat cagccgggtg ttgtgctcgg ccgacatggc
      661 gagctgctcg ggggtctgtc cggcgatctg ccggtagacg agcctggata ccgctgtgtg
      721 cttgcggacc actccgatac cgggcggcga catgaagcgt tccgtcagct cctgcgcgat
      781 cggctcggtc tcgccccggt cgatcatcgc ggcccagcgc ttcatggccg ccgcgtagtc
      841 ggaggggatg cgggtggtca tcccgaccag catcagccgg cgcaccatcg ccgggtagtg
      901 ctgggcgaac cgcagtccga cagcgccccc gtagcacgcg gccaccatgt tgacgcgtgc
      961 cacccccagc ccgtccagga ggtggcgcag ggccgctgcg aggaagtcga caccgtacga
     1021 cgcgggcagg aagtcggccg tgccgtagcc ggggagatcc accgtgatga cctggccgag
     1081 cggcgcgagc cacctctcgt gccgcaccca cgaattgcgg tcctgcgagg agcctccgag
     1141 gatcagcacc ggatcccggc ccggctccgg gcgtccggag acacggccga cgaagcggaa
     1201 cccgtcgaag gtcatctcat gttccgccgg tcctgaggac acccgcgccg ccacgcgctc
     1261 ctgcccaggg acggtcctga cggcctcgga tgtcgactgg ccgcacacga cggccgtgga
     1321 atggggcatg gtggactcct gaagtggggg gaaggaccct tcctaacagg tcttaacgcc
     1381 tcatctgcca ctcgtgacac cttcgagtgc cgttcgcatc agtgttctaa gatcggccgg
     1441 gtcgccagac catccggtcg gagccggccc gacacccccc gcgcccgtgc tcgcacggac
     1501 gaggtccaca ggcccgtccg agcggcaccg acaccgtcga gtacccttcc ggccagggaa
     1561 aaccctcccc gaaggggctt ggaccgcgct gttgtgggga gtcgcacttc cgaggccctc
     1621 gaacctgccc cggcctccga gccggagaga gaaacgtgtg accgttcatc cctcgcgccc
     1681 cgagaccgcc acggccgtcg cggaccgcgg cgccccggaa gccctgcgca gggctcgaac
     1741 agccttgcgc cgtacccccg tatccatatg gaacgacgac gtctccgact gggcggcggc
     1801 gctcacctac tactcgatcc tcgcgctgct gcccgcgctc ctcgtgaccg tcagcgtgat
     1861 cggcctcgcg gcccccggcg ccgtcgactc gctgatcgcc gacatcacct cgtacgcccc
     1921 cgcggaagcc ggcggagccc tgcgcgcctc cttggaggac atggcccacg agcactccgc
     1981 cgtctggctg ctcatcgcca caggagcggt cagcgcgctc tggtccgcct ccagctatct
     2041 cgcggtcttc cggcgggcgc tcaacaccat gcaccaggtg cgcgacacga ggcacccgct
     2101 gcgcaaggca cacaccatcg tcgtcaccgc tgtggtgctg ctggtcctcc tgctgaccag
     2161 cgccgccgtc ctcgtggtga ccgggccact cgcacgcggg atctcgcccg agatcggtgt
     2221 cagcggcgcc gacgcctgga ccatcctgaa gtggcccctg ctgctctgcg tcgtgaccgt
     2281 gctgatcttc atcctcttcc gctccgggcc gcccgcggcc aggggagtgc ggcgcgggct
     2341 gcctggcggg atcctcgccg ccctcctgtg gctggccgcc tcgggcggtt tcgcgctcta
     2401 cgccaccagg ttcggcagtt acagcaggct ctacggctcc ctcgccggag tcgtggtctt
     2461 cctcgtctgg ctctggttca ccaacctctc cctcctggtg ggcgcccagt tcaacgtcga
     2521 gatggcccgc ctcgccccgg acgcgaagcc cgagggtgag cgggcggcac ccgcggggga
     2581 agcggacggg ccgacggcgg cgggacggcc gtgaacgggc cgacggtcac cggtcggccg
     2641 taacgagccg gggcccggca ccccaggtgc cgagccccgc cactctcccg tatgcgcccg
     2701 gccccgtcag gggaaggacg tcacctgcga cggcaccgtg gtgtcgcccg atgtgggtga
     2761 accggtctcg ttgatcacgt ggtcatactg gcccttgccg cccagcgaaa ccaccagcag
     2821 atcgtggaac ttcactcctg gcttctcggg gacctcgaac ccgtggccct ggcggatggt
     2881 cgggtccacg ttgtagtagc agtagctccc gagcccccag ccctcgtggg tggtgaccga
     2941 gtcgtccacc ttgtaggccg cgtagccctt ctggtcgccg ttctggacgg cggcctggtt
     3001 gggggcgtcg taggccttct cgttctggaa gaagatcgtc ctgcccctct ccccgtgcca
     3061 ctccacgtcg tacttgttga agtgctcgac gaagagaccg gtggcgagga cgtcgtcgcc
     3121 gttgacccgc aggccgtagt cggctctgtt ggtctcccag ccgacaccgt cgccgtggtc
     3181 ggcgcgccag atccaggtgt ggtcgacgat ggtgtcgtcg ttgttgatca ccatgctggt
     3241 ggtcgccttg cccgcgcccg cgccgccgat cctgacgaac acgtcctgga cggtggtcgg
     3301 gttggcggag tggtcggcgg aggcgtcctc aggaccgacc tccagcaggg tgtcggagtt
     3361 ctgcgtgccg gcgtcgatga gcagtcccgc cagcttcacc ccgtccacgt cgccgacctt
     3421 catcgcggtc acgccgttgt cggggacgat cgtcgcgtag ccgaggccga gcaccaccgt
     3481 gtccgcgcgg tcgatgtcga tcgtccggtc cacgtggtag accccgggtg tgaacagcag
     3541 gtgcaggccc tgcttgacgg cggcgttgat ggtctccgcc gtggcaccgg gcttgaccac
     3601 gtagaacttg tccagcggga tcgactcgcc ctgcggcgtg ccgccgcccc aactcgtgcc
     3661 gtgcgcgtcg gtgcgcttcg cggggacgaa gaccttgtag tcgtcgccgt ccagatagag
     3721 gaagggcttc tcgcgggaga ccggggtggt gtccagtgtg gtgtagggag gctcggggaa
     3781 gctctgcgcg ggagcaccct cgacacccga gaacaccatg ttccagacgc cgttgcccca
     3841 gccgccgacc tggctgtccc tcgtgtacca ctgctgctgt gagtagggtc ccacgctgcc
     3901 gtcgatcttg ctgtcggcga tgtagccgcc gctggcccag ccgtagccgt cgggggccag
     3961 gttgagtccg cccttgacgt gcatgcgccg gaagggtgcc gcctgggaga cggcccagcg
     4021 gtcggtcccg ctgacgggct tcagcgcgag attctccgcg gagcgccaga agttctgggt
     4081 ggcgttgccg tcgaaccagc ccgcgtccac tgtcacgtca ccgttgatgt tggtgtcgtc
     4141 gggggagaga ccgaggccgg atatcgaggt gtagaagccg agttgggcgt tgatgttgtc
     4201 gtacgtgccc ggcttgaaga gcagttggta gcggccctgc ccgaactggg cggattcctg
     4261 ctgcttgaag atcgcgtcga cctgcccctg gatgtcgggg gtcgaggggt cgaagacctt
     4321 cacgttcggg ccgaggtcac cgccgccggg gacatcggcg gaagcggcgc ccgcgggggc
     4381 gaacaccaga aaggacatca ccagggcgga cacggcggac agcaccgtcg cgcgcctcct
     4441 ggccgaggac ggtggtggtc tgtggaacaa ggatgaatcc gtggggggaa cgtgcatgct
     4501 gattctcctg agtcgtggaa cgggtgcacg acgcgcaacg gagagcgctc tccggttgag
     4561 ggaatgcttc cgctttgttt cggacacgtc aataggtgtg cgtcaacaaa tgggcggggg
     4621 aggaggtcgg tgtggtcctt gtgaacaaga agtggcgcca tatgtgagct gattgttggg
     4681 ctaaatccac tctttcggta tcggatctcc ctccgggccc ctcccgtcgt ttcacaagct
     4741 gacctcgcgg ctccaccggt gacatcgacc cgtgcgggcc tgcctcccct cccgggcgtg
     4801 ccctcgccgc taactcgggc gtgtgcgccg tacggcccaa ggccggccag ggcgggcgga
     4861 accgttgggc cgcggcgtcg ttgtgcgggc gtgcggcggg caggcgaccg tcgccccggc
     4921 gcccacccgc acggaagcgc cggtacgcac cgcgcccggc ctccaggggt cgcggcgcgg
     4981 ccgacgagcg gagagcggcc gtgaccagtc gaaccccatc aggtgacctg ggcccgaagg
     5041 acgggccccc tgacatggcc ccccgaggcg aaacggggaa gcccgatccg gcgacgggaa
     5101 gcgggccgtt ctcgcggcgc ggcgcgctga gggcgttgtt cacggtctca gccgtcgcgg
     5161 gcacgggagc ggccctcgcc cccctgctcg cggcggagac gtcccccgcc gccccgcgag
     5221 ccgccggggc cactgtcctc gcggcccgcg gcggccgggg cggcgagacc ctgttcgacg
     5281 aggtctaccg cgggcgaagg atccagggcc gggcgttgcc gcggggcgcg ggcggtgcga
     5341 aggcgcggat ccaggccacc gtggacggac gggtgctacc cctgacgcgc agggccgacg
     5401 gcggctgtct gagtccggtg gaccactacg actcgtacgc gacccccctg gaggcgaccc
     5461 gcgcggccgt ggacgaactc ggcggggcca ggctctcccc cgccgccgcc aggcaccacc
     5521 actgaccgcc ggaaacaacc cccgaccggc cgagagaggg agaaccgcga tggtgtacac
     5581 ccggaagaac cagcgccacc tgacgaaggc ggagaaacgg aagttcgtcg cagccgtcct
     5641 ggaactcaag cgccgaggcg actacgacgc cttcgtacgc acccatgtcg agtactacgt
     5701 ctccgacggt gacgacggcc ttcgagtcgc ccacatggcc ccgtccttcc tgccctggca
     5761 ccggcagttc ctgctggagt tcgagcgggc cctgcggaag atcgacccga gcgtcacggt
     5821 gccctactgg gactggacca aggaccgtac gccggcctcc tcgctctggt ccgaggactt
     5881 cctcggcggc gacggcagac gcggcgaccg gcaggtgacg acaggcccct tcgcccacgc
     5941 ccgaggccac tggaacatcg tggagggcgt cagcgaggac cccttcctcg ccagggagtt
     6001 cggccgcccc cgcgacccga tcgacctgcc gaccggcgag gaactggcca gggccacggc
     6061 cgaaggcctc tacgatgtgg agccctggga ctccacatcg gaccacgggt tccgcaacaa
     6121 gctggagggg tggaccaggg ggcggggcaa ccagcggttc cgcaaccaca accgcgtgca
     6181 ccgctggatc ggcggcaaca tgctcggcgg cgcctcggtc aacgatcccg tcttctggct
     6241 gcaccactcg ttcatcgact tggtctggtc ccgctggcag cgcgcccacc cggacgccgg
     6301 ctacctcccc gcgaagccgc caccggccgg atcggcgcag tacgggaggg tcgccgcgct
     6361 ggaggatccg atgccgccct ggaacgtcag accgtcggcg ctcctcggcc acgggcacct
     6421 ctaccgctac gcctgacccg cccgccgtcg cgcggccccg gcgctcctcg tgaagtcggc
     6481 ggccgcgaac ccgaaggccg cggacacgct tccgtgagat cgccgggccg gctcccggac
     6541 agccgtacgg ccccggcggc gaacgccgag gccgtacgag aggggtgcgg tgcgctccac
     6601 tcgccactcg tgtggacgat gcggggcggg tcgaccgcga ggacggatca gccgccgtag
     6661 ccgccgtggt ggtggtgaac ggtggtgttg acgcacgtgt taccgaaggc cgggttcagc
     6721 aggccgatga cgttgaccgt gttgccgcag aggttgaccg ggatgtgcac cggaacctgg
     6781 actgcgttgc cggacaggac gccgggggag ccgatagcgg cagcctgggc gcccgcgtcg
     6841 gcggtggcca taccagcgcc ggcggccatg acggcgccgg taccggcgat gacagcggct
     6901 gcctttgcga tgcgggacat taaagatctc cttgaattcg ccaccggccg cgcggctgcg
     6961 gccgtgtact ccccctacaa cgccgcgact cacccttagt aacggaaaac ctcccacttg
     7021 cggcaaacgg gtgatagcgg tcctcgccgt ccacgtgccg gacggcccga tccgttccag
     7081 gctggagatc cgggggcgga acatcaccca cagagaggcg tgaggacgcg tgcagcacac
     7141 cagcaaccgg ccggccgagg agacggcgcg gcgccccccg cccgccgtct ccgaccccgc
     7201 ccaccgctcc gacgtcaccc tgcgcgtcaa cggtgagcgc cacaccgtct ccctggacca
     7261 ccgcaccacg atcctggaca ccctgcgcga ggaactcggt ctcaccggcg ccaagaaggg
     7321 ctgcgaccac ggccagtgcg gggcgtgcac ggtgctcgtc gacggcaggc gcgccaacag
     7381 ctgtctgctg ctcgccgtct ctgtcgacgg agccgacatc accaccgtcg agggagtcac
     7441 ggacggtgag gacctccacc ccctccagga ggcgttcctg cgccgcgacg ccctccagtg
     7501 cggatactgc acacccggtc agatctgctc cgcgctcggt gtgctgggtg aggcggcggc
     7561 gggacacccc tcactggtga ccccggccga cgccccgcac acgggcgagc cgatggagtt
     7621 ggacgcgcgg gagatccgcg agcgcatgag cggcaacctc tgccgctgcg gcgcctatcc
     7681 ccgcatcgtc gaagcagtcc aggacgtgac ccggtgaagc ccttcgccta ccgccgcccc
     7741 cgtaccgcga ccgaggccgt cgacgccctc gcggcccatc cgggcgcgcg cttcctcggg
     7801 ggcggcacca acctcgtgga cctgatgaaa ctcggcgtcg agaccccgca cacgctggtc
     7861 gacgtcagcg gcctgccgct cgacaccgtc gaggagctga ccgacggcag cctgcgcgtg
     7921 ggggccaccg tccgcaacag cgacctcgcc gcccacccgg ccgtacgggc ccgctacccg
     7981 gtcctctccc aggccctgct cgccggtgcg tcaggacagc tccgcaacgt cgccaccaca
     8041 gggggcaacc tcctccagcg cacccgctgc ccctacttcc aggacacctc acagccctgc
     8101 aacaaacgca ccccgggctc cggttgcgcc gccgtggagg ggcacaaccg cgaacacgcc
     8161 atcctcggcc actcgccgcg gtgtgtcgcc accaacccct ccgacctggc cgtcgccctg
     8221 tgcgtcctgg acgcctccgt ggaactgctc gggccagaag gcgagcggac cgtcgccgcc
     8281 gccgacttcc accggctccc cggcgaccac cccgagcggg acagtcacat ccggccgggg
     8341 gaactcatca cggcggtcgt gctgccggcg cgccccgcgg gtctgccctc cgtctaccgc
     8401 aaggcccgtg accgcacctc gtacgccttc gccctggtct cggtcggcgc cgtactcgac
     8461 gtacgcgacg acggcgtgat ccgatacgcc aggctggcct tcggcggggt cgcgcaccgg
     8521 ccgtggcgcg cgctgcacgc cgaggacatc ctctgcggcg cccacgccac cgaggagacc
     8581 ttcacccgcg cgctcgacgc ggagctggac caggcgaggc cgctgcgcga caacgccttc
     8641 aaggtgaccc tcgcccgtcg gctggccacg gacgtactca ccggcctcgc ggaccccggc
     8701 gcgcccctca ccccgtggcc ccacaccaca gagcagtcct gacggtgccc gaccacgaga
     8761 ggaccacacc atgaaccagc caccctcctc gctgggcacc ccagcagtcc gcgccgacgg
     8821 ccgcgccaaa gtcaccggcg cggcccgcta cgcggccgaa cacaccccac ccggctgcgc
     8881 ctacgcctgg ccggtgcccg ccacgatcgc ctcggggcgt gtcaccgccg tccacgcgga
     8941 cgaggcactg gccctgcctg gcgtggtcac cgtcctcact cacgagaacg cgccgcgcct
     9001 gacgcccggc gacgaaccca tcctgctcgt gctccaggac gcctgcgtgc cgcactccgg
     9061 ctggttcgtc gccctcgtgg tggccgacac cctcgaagcg gcgcgggccg gagcgcgcgc
     9121 cgtgcgcgtc gagtacgaga ccgacccgca cgatgtcctg ctgcacgcgg gccacgccgg
     9181 catcaagcag cccgctgagg ccaacggggg gttccccgcg gaccgcgaac gcggcgattt
     9241 cgccaccgcg ttcgccgagg cacaggtgcg cgtcgacgcc acctacacga tgaccccgct
     9301 gcacaaccat ccgatggaac cgcacgccgc cgtcgcccac tggcacgaag gacacctgac
     9361 ggtccacgac agcagtcagg gcgccaccgc cgtccgcacc gcgctcgccg ggctcttcga
     9421 ccggcccgag gagacgatca ccgtcatctc cgagtacgtc ggcggagggt tcggctcgaa
     9481 aggcaccccg cgtccccagt gcgtcctcgc cgccatggcc gccagggaga tcggcaggcc
     9541 cgtcaaactc tccctcccca gaggccagtt gcccgccgtc gtcggccacc gcgcgcccac
     9601 gctgaaccgg ctcagactcg gcgcggccca ggacggtgtc atcgccgcac tggcccacga
     9661 ggtgctcacg tacacctcac ggatcaagga gttcgtggag caggcggccg taccggcacg
     9721 catcatgtac cactcgccgc acagcctcac cactcaccgg gtcgccccgc tggacgtctc
     9781 cagtccgtcc tggatgcgcg cccccggcga ggcctcaggg atgtacgccc tggagtcggc
     9841 catggacgaa ctcgcccggg ccgcgggggt cgaccccgtg gaactgcgga tccgcaacga
     9901 gccgtcgacc gatcccgaca gcgggcgacg cttcagcagc cggaacctcg tcgcctgtct
     9961 gcgtgagggg gcggaacggt tcggctgggc ggcccgcgac ccgaggccgg gcgtccgccg
    10021 cgaaggcgcg ctgctggtcg gcaccggagt cgccgccgcc acctacccgg tgttggtctc
    10081 gccctccaag gccgaggtgc tcgccctgcc cgacggcacg ttcctcgtca ggaccaacgc
    10141 caccgacatc ggtacggggg cccgtacggt cctcgcccag atcgcggccg acgcgctgcg
    10201 ggcaccactc gactccgtgc gcatggagat cggcgacagc gatctgccgg ccgccccgct
    10261 cgcgggcggc tcctccggca ccgcctcctg gggctgggcg gtccaccagg cctgcaccga
    10321 actgctggaa ctgctggggg agtacggggg agagatcccg cccggcgggc tcggcgcgag
    10381 cgcggacacg gaccagcaga ccaacaccga cgacgcgtac tcccgtcacg ccttcggcgc
    10441 gcacttcgcc gaggtgcagg tggacaccgc cacgggcgag gtcaggactc gcaggctcct
    10501 cggggtctac gccgcgggcc atgtc
//
