LOCUS       NZ_CP045178            21536 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7407378
            Orig. end                         :: 7428914
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21536
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="30"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21536
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21536
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11536](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11536)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(34..3960)
                     /locus_tag="GBW32_RS31200"
                     /old_locus_tag="GBW32_31190"
     CDS             complement(34..3960)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS31200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_31190"
                     /product="hypothetical protein"
                     /protein_id="WP_077974515.1"
                     /transl_table=11
                     /translation="MTTTAQPTPLIVPVQISAMAVNGRVREETFFRQAPHFPRPPAYRE
                     DPEPDFTTFDADFQTLDSSDGVYLQWELPAAAVRGRADTARIEPAADAGAGDTEAVVYP
                     VLPNRWMIVRYHRPGTSTATDPPSAAGWLLWSDHVDPDGGSPFPAPGATDSDDVAGASM
                     YVGTAHSLDADGWKPQGGERFLPVTALGAGIPGFTVYQPYNANILSFHDPLRTNGGKGD
                     LLPAGTVSYLVAGWHQDHADDLLAPEALKSLLLFRNGEGSIPAVPEELVAAGLRALNWT
                     LPPPVGGPAPAPQRALYHGLLLGLPWQNTKHFPSDRPQATDTVLYALGNSALDARSALP
                     ATTALAPRDRLLHRAFAQDCLDAFATALDDGRNTLDAAALATWFLASETGYRWQITDSP
                     QQAGIKPPAKPNAQELAKERDWLTRLNADQNAYDARLRDLAEAQTRLYDLWWLAGTDPE
                     KQPQDFAARAREQLAPDRPGTLAAQVMALRNECFGLQGLRQKVPHGATTHDLAAAITAY
                     ANADIRKLPAGRRLKRVPLPPYHRPADPLVLIKGAGTPLTAYPTTPTPCRLPGQLIDHD
                     GITGRPTTPPPAPTRFGELIADLPWAPLDGLLSEFRALEDLTRRVFAHWPTADDPAPPD
                     LIEDAQTLGWKPVTAAGGTPEWPAPCVLWRQPWSPLMVLWTATIHPLPYLSAGTVPAAK
                     AAPTENWAFDGARYRWRGTGSLPDPPTLVGHSLLTDLPAFVLNGRVTQYLARRPDAPAN
                     LLRALAEEAGDEICQTLDGINAALARRVRSNHHEPLDGPAAALLSTRLLVPVPSRLPDP
                     AAQHFEPVRAGQIVLTRLTVVDRFGRSVNILDPMTPELSPDPRLADSVKPNGDPAGHIT
                     GVLAVPGETRSKCVQLTPRLQQAARTRFDTVCARDDATVINPDDDLPSENPGPLCGWLV
                     PDHRADTLLVYGAGGEPLGELLATAPDDEHYQVDWTPLPGSPWMTREDVLGAQFAAAYP
                     HLCGILATVLGPGESGGPGATGWLRAHAELHGTLDEGLLHIAPAHRGEDPAWSLVGGRP
                     VAVVRARLGIELAASPLPAADWDRLLTGPAENDGNRLRDIRWPIRLGDSTRPDDGLIGF
                     FTADRDTPDQTATNYRRLYTPYPPGGTAGEYARGLDPATDPAVRARRADTPPTTGDHTL
                     SWVTLLIDPWTSATATTAILPAVSARLPTACVDGPRSRLTVSLRIGPLLAGTATPTTPN
                     DTDPKPTRGLALPKPSPRYGTWAWSERTPPQETTLWASLPINGPSTAVHPPDYPPDART
                     GHLTLTSPAPLSTSTSRPGPEDPQ"
     gene            complement(3957..6224)
                     /locus_tag="GBW32_RS31205"
                     /old_locus_tag="GBW32_31195"
     CDS             complement(3957..6224)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS31205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_31195"
                     /product="hypothetical protein"
                     /protein_id="WP_143621626.1"
                     /transl_table=11
                     /translation="MSQSVAVAGQADPGYEPPPPYTQSFEVRGPRYTLDPQLVCQVYPR
                     PGSLGSVADVLPHAALSLSTLPWARPVKDTDPHIPWLALLTFADGELTVDPATGQTVST
                     RDIAKLADPEPGVLVPHLAPETMPADTTTCRTIDVPAPLFTALTPRLAELRHLVHVKHV
                     DAVTTWSGRNATDLEIEEPEPAEVAVVLSNRLPRAGSGHGGGFSVHLVSVEGFADHLDG
                     KPVPVPFTRVRMISLYSWTFTAQGEVHPDFADEAENLKTGSGTDLLLRAQPRTSNTRSG
                     NESGEAGGAEADGGDGTYLKDRLAQGYVPVAHHLPTGERTVAWYRGPWTPVTPHQVPYP
                     DDGPATEADALVYLPEHGMFDVSHASAFSLGRTLALSSDDLPTRLSRMRADGVLLAQEI
                     TAAGGPDAAGPLLDLLAGADPDEDGRTVPPDVLNRLRAAHRVHRAFEESLHTPAPGSPR
                     DTAASAAPGVGLRAVVESSALSPAGQLLDLLVESHLGPIRRAGLDANTLLASVPFDHLV
                     PHAALLPPDSVRFFTLDTQWLHVMAAGAASVGRSTSLDTRLTARLITRLRERADPPSYG
                     ALIRSPLVRDWPTLTVTATGRDGRDVLHGPPQSLTPDLMLLSFREAPAQVALREPAEGI
                     RFGVDGDHTIELRRLKDSGPSKQVGMSIDCTADITSCLRDATRGVLTIWTDPDTGGPCL
                     NRVLRDKLAHFSEPQWDSGSDLTAAALSVQLLNGPHQLVFTATSSASIGEDGARDLREP
                     SR"
     misc_feature    6278..6292
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.48"
                     /tool="antismash"
     gene            complement(6365..8899)
                     /locus_tag="GBW32_RS31210"
                     /old_locus_tag="GBW32_31200"
     CDS             complement(6365..8899)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042646.3"
                     /locus_tag="GBW32_RS31210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31200"
                     /product="DUF6603 domain-containing protein"
                     /protein_id="WP_077974513.1"
                     /transl_table=11
                     /translation="MRVLLVGAEVSGGLSNLPVIGPHVPPDAVRLRGLYAAAANRGLGP
                     DEAAKVNALLGAVDHSGTGPRVPAEGLTGTLQLGVDVHVGDRDLILTATGGKGTAARTG
                     LGNDTAITWYAVGRSLGPVRLDRLGVQYSDHRVWLLLDAGISGDALTISAQGMGLGIGL
                     TQPPTVEARLDGLGLAWDRSPIKISGALLNRHTPNYLIDVEGAAAVITPALSLGAVGGY
                     AQPLTGEPSVFVYGQLAFKEGEGIGPPPFRITGAAAGFGYNSAVRVPEAGDVEKFPFMP
                     GNAQGVTTDPLEYLEKLTGGSTPWVTRTPGRMWLAAGLQFDSFEFLHGRALALVQFAVT
                     GRTDFTATLLGQVGAQFPVAPATAYANVALDVMASYTSSEDTLEIYAAIDPASFLVHHS
                     CHLTGSAALRLWFGNSAHPGDFVLAAGGYGPGYDKPDHYPDVQPIGLSWSVSDHISVRG
                     SCYAALVPSSFRVGGRLDVSFEAGPISAGLHASLDAMVQRDPFYFRVSIHVDVWVEVDL
                     WVATLRGSFGVGLDLWGPPTGGIASVHALGRSFDIRFGEDPPVHQPRLSWQEFTERLLP
                     RPVAHVVAERGLLPGVPPSQAATMRRQPAMAQGPEPWTLSPHGFGLLTRTAIPANQLRV
                     ADAEGHWQKVPSTSDRAAIKIRPMGVDGADSTHQIAVQRDTHGYNPLSPDAAWVFTPVE
                     GKVPEGLWGTPITDAGPGASTGEALIDAWTGVRVTTPEPRLSDNPVTATENAVAAPEQI
                     TGPPMPLPTQQWPGIGPDHATRVRHSIADPAGGITASVTTSAREDLCLALDRAGVPVLS
                     EGRPALRDPLVEYREHLRDHLRSEPLWLSERP"
     misc_feature    complement(8943..8962)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 24.17"
                     /tool="antismash"
     gene            complement(10001..11536)
                     /locus_tag="GBW32_RS31220"
                     /old_locus_tag="GBW32_31210"
     CDS             complement(10001..11536)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012647.5"
                     /locus_tag="GBW32_RS31220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31210"
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="WP_077974512.1"
                     /sec_met_domain="T2TS (E-value: 6.7e-48, bitscore: 162.1,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRARPTRTRPWPPTTLPHDHTRRVDLLRTRVMDSVTPSGAVRGHC
                     ESRVLESALLLALLRRTRTTPQAARHLEQFLSARTTGGDRIDDVLARAATGNLRREDDV
                     KIRALVDEAPEFMRTRKHALIAATLCLLGRPQPHVAPPPIPAQGMHPAGRVQLIAIRVI
                     LAHAAGRPAPAAAHELLPATSAPRVWEGNLLVHLLAANALSLLPGHEAAVAGGIRQALA
                     CRRPDGGVPFVTGVDTWCTVTAGIALASCGAPTAVQHRLARHLAAQQRADGGWSYTDHA
                     HHTDTDDTAVAVEFLHTLDPTRYRTPIDRGLAHLRSLQGPDGGFPTYVAGARSEAGMTA
                     AAVNALSIRPSQHRPHIHAALDFLTRAQRPDALFPPEWSRSRFHVAFRALLAADAPYQR
                     PAPHGTDRRMVRTLLTAVLHHRNSDGGWGQQPGECSDAISTAYALLCLCRHHAPRPAIE
                     AATFLLDTQRDDGSIASRPDMLGTRPFLYNVPRLADHFALLALGHLHQRLHGT"
     misc_feature    complement(10205..10207)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11789..12445)
                     /locus_tag="GBW32_RS31225"
                     /old_locus_tag="GBW32_31215"
     CDS             complement(11789..12445)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS31225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_31215"
                     /product="hypothetical protein"
                     /protein_id="WP_077974511.1"
                     /transl_table=11
                     /translation="MIDAALRLDPTRVYAFSGSRYVVFDPSDPHAHSEPRPIVEGLPAL
                     AGTVFAHGVDAAASKSPTPGAPNEAYLGRGEVFVRYNLDDDTLTAGPSAIGSAWSALTR
                     SGFDRDVDAAMRLDTDHVRLFKGSRCLTLNLRDKSVEVGPQPIAECFPGLAGTDFAADL
                     GAVAERDPDDSGDSRIYFFKNGSYLKYDASLDAVAGGPVRVSDAWPRLREAGFGA"
     gene            12755..13105
                     /locus_tag="GBW32_RS37880"
                     /old_locus_tag="GBW32_31220"
     CDS             12755..13105
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014908.5"
                     /locus_tag="GBW32_RS37880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31220"
                     /product="hypothetical protein"
                     /protein_id="WP_077974510.1"
                     /transl_table=11
                     /translation="MYEGPGAASLATYAAAYSPKETIIDFTEPAAVICRKVAALTVAVP
                     WAQMSVRGCEAVIVKAELAESDGAAVPGTLLASHSDGWTVQAADGAVRLTGTRSRARNR
                     TTSRPGCGPSVH"
     gene            13401..14036
                     /locus_tag="GBW32_RS31235"
                     /old_locus_tag="GBW32_31225"
     CDS             13401..14036
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS31235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_31225"
                     /product="hypothetical protein"
                     /protein_id="WP_077974509.1"
                     /transl_table=11
                     /translation="MNKSSLRLAALMVGSLAVLAAVVPPVSAGEAGGVLPPLPLKAGDP
                     YYFCLTDATETALAKARISLQAVSPNTVVTYKAHTCMRGTLTSGQINTDLTGLSGEGTG
                     GFAFQRDAERAEFVHPRVTLSPDMTGSWTAEHAGRRIEMFTSTKVGAKFSLTKVSATDL
                     PMTLTKQGADVLADTFGTSPLPAGTPFFEGNASFDVLNSLTAPLTTNP"
     gene            14144..15376
                     /locus_tag="GBW32_RS31240"
                     /old_locus_tag="GBW32_31230"
     CDS             14144..15376
                     /NRPS_PKS="Domain: Condensation_Starter (83-247). E-value:
                     3.4e-10. Score: 31.7. Matches aSDomain:
                     nrpspksdomains_GBW32_RS31240_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555368.1"
                     /locus_tag="GBW32_RS31240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31230"
                     /product="phthiocerol/phthiodiolone dimycocerosyl
                     transferase family protein"
                     /protein_id="WP_227025367.1"
                     /sec_met_domain="Condensation (E-value: 7.3e-05, bitscore:
                     20.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQMMVERLLAPHEEAIDAAGIRLVLHTELDGDLDEATLAQALVHL
                     HECYPLSAGRITTRAGEGRAIVRVEERAAAPVLGRGSDIREEISAPLDWEQGPLLRVSV
                     VRDGRTRVVMTLPRAFADAMSYQALHQRLWTIYTALATGRPVPAEPVRPALGPALDDVL
                     AARFTPSKLRDFVAERARLDTQAPPALLPALASQGGGPGTDLNFGITSVEAGPDLCEKL
                     LQRARNASLSLNSLVSGALLTSLRTLFPTHGEPVRMLCTMAVDMRRRLTPPMPAEILQS
                     AATTTSMRLWIDSRARPEDVGRNVAAQLRADLDSGAAAMELAAFPYMLDQHPPSLVITN
                     VGTIAEPPLPQGLRISRVRLAPLGHVPMIFAVISRYQGRLTIDLTHSRAWYTETQIKEL
                     SGHLSATLDAT"
     CDS_motif       14225..14278
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GBW32_RS31240_0001"
                     /evalue="4.20E-04"
                     /label="C2_starter_024-063"
                     /locus_tag="GBW32_RS31240"
                     /protein_end="45"
                     /protein_start="27"
                     /score="13.0"
                     /tool="antismash"
                     /translation="ELDGDLDEATLAQALVHL"
     aSDomain        14393..14884
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS31240_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="3.40E-10"
                     /label="GBW32_RS31240_Condensation_Starter.1"
                     /locus_tag="GBW32_RS31240"
                     /protein_end="247"
                     /protein_start="83"
                     /score="31.7"
                     /specificity="C activity: inactive"
                     /specificity="Active site motif: PRAFADA"
                     /tool="antismash"
                     /translation="IREEISAPLDWEQGPLLRVSVVRDGRTRVVMTLPRAFADAMSYQA
                     LHQRLWTIYTALATGRPVPAEPVRPALGPALDDVLAARFTPSKLRDFVAERARLDTQAP
                     PALLPALASQGGGPGTDLNFGITSVEAGPDLCEKLLQRARNASLSLNSLVSGALLTSLR
                     T"
     misc_feature    complement(15685..15699)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.31"
                     /tool="antismash"
     misc_feature    15686..15697
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.87"
                     /tool="antismash"
     misc_feature    complement(15692..15706)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.12"
                     /tool="antismash"
     gene            15855..>16085
                     /locus_tag="GBW32_RS37885"
                     /pseudo=""
     CDS             15855..>16085
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012870.5"
                     /locus_tag="GBW32_RS37885"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRDLDLTPCQPRPWRPAPTDPDRRHRLPDLIRRDFLATTPGTKLV
                     GDITYIPTWEGFLYLATVIDCCTKKVIAWAMA"
     gene            16130..16477
                     /locus_tag="GBW32_RS37890"
     CDS             16130..16477
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF025063.5"
                     /locus_tag="GBW32_RS37890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="integrase core domain-containing protein"
                     /protein_id="WP_405520752.1"
                     /transl_table=11
                     /translation="MAALNTPLTPGAIFHSDRGSNYTSDAFARKLKSLNLRQSVGRTGV
                     CWENAMAESFFSTLKNEWLNRFEFTTRSKARTRVVRYIEGFYNPKRLHSALGYRAPQEI
                     EDEHQQPPLVA"
     gene            complement(16911..18176)
                     /locus_tag="GBW32_RS31250"
                     /old_locus_tag="GBW32_31240"
     CDS             complement(16911..18176)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993112.1"
                     /locus_tag="GBW32_RS31250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31240"
                     /product="IS701 family transposase"
                     /protein_id="WP_152330828.1"
                     /transl_table=11
                     /translation="MLSEELAAVRCDLEDFAAEMFEPFARADQRRWGGVYLRGLLLDGG
                     RKSVEPMAARLGEDGNRQALAHFVTSSPWDAAHVRARLAWHMQPVIKPTALVIDDTGFL
                     KDGDASACVTRQYTGTAGKVTNCQAGVSLHLASNGASAAVNWRLFLPGSWDPASPKADR
                     ATVARRDKCAIPAQVGHVEKWQLALDMIDETRSWGIEVPQVIADGGYGDTAAFRIGVEE
                     RGLDYVVGISTTTTAQPEDAQPCTPAYSGRGPRPVPAYPEPAQQVKSLVIAAGKSSARP
                     VQWREGSRPGSGRSGFKRMYSRFVALRIRPAGREIRKATPGSEFPVRWLLAEWPADQDA
                     PVQFWLSNLPETTPLPVLVRTAKLRWRIENDYREMKQALGLAHFEGRTWPGWHHHVTLV
                     SVAHAFCTLQRLNRSPKETASA"
     gene            complement(18345..18584)
                     /locus_tag="GBW32_RS31255"
                     /old_locus_tag="GBW32_31245"
     CDS             complement(18345..18584)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735180.1"
                     /locus_tag="GBW32_RS31255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31245"
                     /product="chaplin"
                     /protein_id="WP_077974565.1"
                     /transl_table=11
                     /translation="MKKTIAVLAVSAAVLLGSAGIASADSDAIGAAVGSPGVISGNATG
                     APVGLPVGACGFNVDAGGALNPSADAACTNTFDD"
     misc_feature    complement(18889..18899)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            19295..20071
                     /locus_tag="GBW32_RS31260"
                     /old_locus_tag="GBW32_31250"
     CDS             19295..20071
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024578.5"
                     /locus_tag="GBW32_RS31260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_31250"
                     /product="PDZ domain-containing protein"
                     /protein_id="WP_077974566.1"
                     /transl_table=11
                     /translation="MHRSRSPWALTGPAATLTAVLVAVALPPAHAVTAAPAHTPAAVSH
                     APRAVMIPPQQDVPELNAPGDPVDVVFPPDNRTRPGTGRFTVTAPPHTRITALGMRCLT
                     NSSCSAAIAGDGSTATVTLRRGRWTFSSPVTVTLQASPSAPLPGGRYAGVFTAGNESQP
                     LTVEITEGNQGTLGISHKDAPDRGGAQVVFVGRGGAADTAGIREGDIITSINNTPVTTS
                     NELRNARLGKLRQGATIPVTYRQPNGTTRTTQVTLT"
     gene            <20225..21020
                     /locus_tag="GBW32_RS31265"
                     /old_locus_tag="GBW32_31255"
                     /pseudo=""
     CDS             <20225..21020
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1237: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037846392.1"
                     /locus_tag="GBW32_RS31265"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="GBW32_31255"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="ELIDGITAELTVLAGFDQDLAAEATRTSNRIRGLLTQFPPSLERV
                     LGPRLDHRAVTWLLERHGSPAALRKAGRRRLVTLIRPKAPRMATRLIDDIFDALDEQTV
                     TVPGTGTLDTVIPSLARSLAAVHEQRRALETRISALLEAHPLSPVLTSMPGAGVRTAAV
                     LLTTVGDGTSFPTAAHFASYADLAPTTKSSGTSIHGEHAPRSGNRQLKRTMFLSAFAC"
     misc_feature    21458..21472
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
ORIGIN
        1 cgtctgtcag gtcggtgggt gggccgggat gggtcactgt gggtcttccg ggccgggccg
       61 tgaggtgctg gtggacaggg gggcggggct ggtcagtgtg aggtgtccgg tgcgtgcgtc
      121 gggtgggtag tcgggcgggt ggacggctgt gctggggccg ttgatgggca gcgatgccca
      181 cagcgtggtt tcctgcggcg gggtgcgttc gctccaggcc caggtgccgt atcggggtga
      241 gggtttgggc agagcgaggc cgcgggtcgg cttggggtcc gtgtcattgg gggtggtggg
      301 ggtggcggtg ccggccagca gggggccgat gcgcaggctg acggtgaggc gggagcgggg
      361 accgtcgacg caggcggtgg gcaggcgggc gctgacggcg gggaggatcg cggtggtggc
      421 ggtggcgctc gtccaggggt cgatgaggag ggtgacccaa ctgagggtgt gatcgccggt
      481 ggtgggcggg gtgtcggccc tcctggcgcg gacggcgggg tcggtggcgg ggtcgaggcc
      541 gcgcgcgtac tcgcccgcgg ttccgccggg cgggtagggg gtgtagaggc ggcggtagtt
      601 cgtggcggtc tggtctgggg tgtcgcggtc ggcggtgaag aagccgatca ggccatcgtc
      661 gggccgggtg gaatcgccca ggcggatcgg ccagcggatg tcgcgcaggc ggttgccatc
      721 gttttcggcg gggccggtga gcaggcggtc ccagtcggcg gccggcagcg gtgacgcggc
      781 cagttcgatg ccgagccggg cgcgtacgac ggcgacgggc cgtccgccga ccaggctcca
      841 ggccgggtct tcgccgcggt gtgcgggggc gatgtgcaga agcccttcgt cgagggtgcc
      901 gtgcagttcg gcgtgggcgc gtagccaccc tgttgcgccg gggccgccgg attccccggg
      961 ccccaggacg gtggcgagga tgccgcacag gtgcgggtag gcggcggcga actgtgctcc
     1021 caggacgtct tcgcgggtca tccaggggga gccgggcagg ggcgtccagt cgacctggta
     1081 gtgctcgtcg tcgggtgcgg tggccaggag ttcgcccagc ggttcgccgc cggcgccgta
     1141 gaccaggaga gtgtcggcgc gatggtcggg cacgagccat ccgcacagcg gcccggggtt
     1201 ctccgagggg aggtcgtcgt ccgggttgat aacggtggcg tcgtcacggg cgcagacggt
     1261 gtcgaagcgg gtgcgggcgg cctgttgcag gcgcggggtc agctggacgc acttgctccg
     1321 cgtctctccc ggcacggcca gcactccggt gatgtgtcct gcggggtcgc cgttgggttt
     1381 gacgctgtcg gcgagccggg ggtcggggct cagctcgggc gtcatcgggt cgaggatgtt
     1441 cacggagcgg ccgaagcggt cgaccacggt caggcgggtg aggacgatct gcccggcacg
     1501 cacgggttcg aagtgctggg cggcgggatc ggggagacgg ctggggacgg gcacgaggag
     1561 gcgggtgctc aggagtgcgg cggcggggcc gtcgaggggt tcgtggtggt tggagcggac
     1621 ccggcgggcg agggccgcgt tgatgccgtc cagggtctgg cagatctcgt cgcccgcctc
     1681 ctcggcgagg gcgcgcagca ggtttgcggg ggcgtcgggc cggcgtgcga ggtactgggt
     1741 gacgcgcccg ttcaggacga acgcggggag atcggtgagc aggctgtggc cgaccagggt
     1801 cggcggatcg ggcagagatc cggtcccgcg ccagcggtaa cgggccccgt cgaaggccca
     1861 gttctccgtc ggcgccgcct tggcggcggg cacggtgccg gcactgaggt acgggagggg
     1921 gtggatcgtc gcggtccaca gcaccatcag tggcgaccat ggctggcgcc acaggacgca
     1981 gggggcgggc cactccgggg tgccgccggc cgcggtgacg ggtttccagc ccagtgtctg
     2041 cgcgtcctcg atcaggtcgg gcggtgcggg gtcgtccgcg gtgggccagt gggcgaagac
     2101 gcggcgggtg aggtcttcca gggcgcggaa ctcgctcagc aggccgtcga ggggggccca
     2161 gggcaggtcg gcgatcagtt ccccgaaccg ggtgggtgcc gggggcggtg tggtggggcg
     2221 gccggtgatg ccgtcgtggt cgatgagctg tccgggcagc cggcacggtg tgggtgtcgt
     2281 ggggtaggcg gtgaggggag tgccggcgcc cttgatcaag acgagcgggt cggccggccg
     2341 gtggtagggc ggtagcggga ctcgcttgag ccgccggccg gcggggagtt tccggatgtc
     2401 ggcgttggcg taggcggtga tggcggcggc gagatcgtgg gtggtggcgc cgtgcgggac
     2461 cttctggcgc agtccctgca ggccgaagca ttcgttgcgc agggccatca cttgggcggc
     2521 gagggtgccc ggcctgtccg gggccagttg ttcgcgcgcg cgtgcggcga agtcctgcgg
     2581 ctgtttctcc gggtcggttc cggcgagcca ccacaggtcg tacagccggg tctgtgcttc
     2641 ggccaggtca cgcagccgtg cgtcgtaggc gttttggtcg gcgttgaggc gggtgagcca
     2701 gtcgcgttcc ttggcgagtt cctgggcgtt cggcttcgcg gggggcttga tgccggcctg
     2761 ctgggggctg tcggtgatct gccagcggta gccggtctcc gacgcgagga accaggtggc
     2821 cagggcggcg gcgtccagag tgttgcgtcc gtcgtccagc gcggtggcga aggcgtcgag
     2881 acagtcctgg gcgaacgcgc ggtgcaggag acggtcccgg ggagccaggg cggtcgtggc
     2941 gggcagcgcg ctgcgggcat cgagtgcgct gttgccgagg gcgtagagca ccgtgtcggt
     3001 ggcttggggg cggtcggagg ggaagtgttt cgtgttctgc cagggcagtc cgagcaggag
     3061 gccgtggtag agggcgcgct gcggggcggg cgcgggtccg ccgacgggag gggggagggt
     3121 ccagttcagg gcgcgcagtc cggccgcgac gagttcctcg ggtacggcgg ggatgcttcc
     3181 ctctccgttg cggaagagca gcagggactt cagcgcctcc ggggccagga ggtcgtcggc
     3241 gtggtcttgg tgccagccgg cgacgaggta ggagacggtt cctgccggca gcaggtcgcc
     3301 cttcccgccg ttcgtgcgca acgggtcgtg gaaggagagg atgttggcgt tgtagggctg
     3361 gtagacggtg aagccgggga tgccggcgcc cagggcggtc accggcagga atcgctcgcc
     3421 gccctggggt ttccagccgt ccgcgtccag gctgtgcgcc gtgccgacat acatggatgc
     3481 gcccgcgacg tcgtcgctgt ccgtggcgcc cggggcgggg aaggggcttc cgccgtccgg
     3541 gtcgacgtgg tcgctccaca gcaaccatcc cgcggccgac ggcgggtcgg tggccgtgct
     3601 ggtgccgggg cggtggtagc gcacgatcat ccaccggttg ggcagcacgg ggtagacgac
     3661 ggcctccgtg tctccggccc ccgcgtcggc agcgggctcg atacgagcgg tgtccgcgcg
     3721 cccccgtacc gctgccgccg ggagttccca ttggaggtac accccgtcgc tggagtccag
     3781 cgtctggaag tcggcgtcga aggtggtgaa gtcgggctcg gggtcttcgc ggtaggcggg
     3841 cggccggggg aagtgcggcg cctggcggaa gaatgtctcc tcgcggacgc gcccgttgac
     3901 ggccatcgcg ctgatttgga cggggacgat gagcggggtg ggctgcgcgg ttgtggtcat
     3961 cgtgagggtt cccggaggtc gcgtgcgccg tcttcgccga tcgaggcgga gctggtggcg
     4021 gtgaagacga gttggtgggg gccgttgagg agttgcacgg acaacgcggc cgcggtgaga
     4081 tcgctcccgg agtcccactg gggttcactg aagtgggcca gcttgtcgcg cagcacccgg
     4141 ttgaggcagg ggccgccggt gtccgggtcc gtccagatgg tcaggacacc tcgggtggcg
     4201 tcccgcaggc aggaggtgat gtcggcggtg cagtcgatgc tcatcccgac ctgtttcgac
     4261 ggcccggagt ccttcagccg ccggagttcg atcgtgtggt cgccgtctac gccgaagcgg
     4321 atcccctcgg ccggctcacg cagcgccacc tgtgcggggg cttcgcggaa actcaggagc
     4381 atcaggtcgg gggtgaggct ttgcgggggg ccgtgcagga catcgcggcc gtcccggccg
     4441 gtcgcggtga ccgtgagcgt gggccagtcc cgcaccagtg gggagcggat cagcgccccg
     4501 taggacggcg gatcggccct ctcccgcagc cgcgtgatca ggcgcgcggt gagacgggtg
     4561 tccagcgagg tgctgcggcc cacgctcgcg gcgccggcgg ccatgacgtg cagccattgc
     4621 gtgtccagcg tgaagaaccg cacgctgtcg ggcggcagca gcgccgcgtg cgggaccagg
     4681 tggtcgaacg gcacggatgc cagcagagtg ttcgcatcca ggccggcccg gcggatcggg
     4741 ccgaggtggc tctcgacgag caggtccagg agttggccgg ccggtgacaa ggcggaactc
     4801 tcgaccacgg cacgcaggcc cacgccgggt gcggcggagg cggcagtgtc acgggggctg
     4861 ccgggcgcag gggtgtgcag tgactcttcg aaggcccggt ggacccggtg ggccgcgcgc
     4921 aggcggttga gtacgtcggg cgggacggtg cggccgtcct cgtccgggtc cgccccggcc
     4981 agcaggtcca ggagggggcc cgcggcgtcg ggtcctcctg ccgcggtgat ctcctgggcg
     5041 agcaggacgc cgtccgcccg catccggctg aggcgggtgg gcaggtcgtc gctggacagg
     5101 gcgagggtgc ggccgagtga gaacgcgctc gcgtggctga cgtcgaacat gccgtgctcg
     5161 ggcagataga ccagggcatc cgcttcggtt gcgggtccgt cgtccggata gggcacctgg
     5221 tggggggtga cgggtgtcca ggggccgcgg taccaggcga cggtgcgttc cccggtgggc
     5281 agatggtgcg cgacagggac atacccctgg gcaaggcggt ccttgaggta ggtgccgtcc
     5341 ccgccgtccg cttccgcgcc acccgcctca ccggactcgt tgccgctccg ggtgttgctc
     5401 gtgcgcggct gggcccgcag caggagatcg gttccactgc cggtcttgag gttctcggcc
     5461 tcgtcggcga agtcgggatg gacctcgccc tgtgcggtga aggtccagga gtacagcgag
     5521 atcatccgga cacgggtgaa gggaaccggc accggcttgc cgtcgaggtg atcggcgaag
     5581 ccctcgacgg agaccaggtg gacgctgaaa cccccgccgt gccccgagcc ggcgcgagga
     5641 aggcgattac tgaggacgac ggcgacctcg gccggctcgg gctcctcgat ctccaggtcg
     5701 gtggcgttgc gccccgacca ggtcgtgacg gcgtcgacgt gcttgacgtg gaccagatga
     5761 cgtagctctg ccagacgcgg agtgagcgcc gtgaacaacg gcgcggggac atcgatggta
     5821 cggcaggttg tggtgtccgc cggcatggtc tccggggcca ggtgagggac caggactccg
     5881 ggctcggggt ctgccagctt ggcgatgtcg cgggtgctga cggtctgccc ggtggcggga
     5941 tctacggtga gttcgccgtc ggcaaaggtc agcagcgcga gccacgggat gtgcgggtcc
     6001 gtgtccttga ccgggcgggc ccacggcaga gtggagaggg agagcgccgc atgcggcagc
     6061 acgtccgcga cactgcccag gctgccgggc cgcggataga cctggcacac cagctgcggg
     6121 tcgagggtgt agcgcggccc gcgcacctcg aacgactgcg tgtacggcgg cggtggctcg
     6181 tagcccgggt cggcctgccc ggccactgcc acactctggc tcaccgtgag ggtgtagctt
     6241 ccggcctcga ccgggggaag cgcccgctcg tggaggtgga aggtccggtc cgccgggggg
     6301 cctgcggcgg ggggccgacc gggtgccgcc gtggcggcac ccgtggtggt cggggtcatg
     6361 gcggtcacgg cctctcggac agccacagcg gttcgctgcg caggtggtcc cgtaggtgtt
     6421 cgcggtattc gacgagaggg tcccgaagtg cgggccggcc ctcggacagc acgggtaccc
     6481 ccgcgcggtc cagagccagg cacaggtcct cgcgcgcgct ggtggtcacg gaggcggtga
     6541 taccgccggc gggatcggcg atcgagtggc gtacgcgcgt tgcgtggtcg ggcccgattc
     6601 cgggccactg ctgggtgggc agtggcatcg ggggacctgt gatctgctcg ggggcggcaa
     6661 cggcgttctc cgtggcggtc acagggttgt ccgacaagcg cggttctggt gtggtgacgc
     6721 gtacccccgt ccacgcgtcg atcaatgcct caccggtcga cgcgccgggt ccggcgtcgg
     6781 tgatcggtgt tccccacagc ccctcgggca ccttgccctc gaccggggtg aacacccatg
     6841 cggcgtccgg ggacagtggg ttgtagccgt gtgtgtcgcg ctggacggcg atctggtggg
     6901 tggagtcggc gccgtccact cccatcgggc ggatcttgat cgcggcgcgg tccgacgtgg
     6961 acggcacctt ctgccagtgg ccctcggcat cggccacgcg cagctggttg gcgggaatcg
     7021 cggtgcgggt gagcagaccg aagccgtgcg gagacaacgt ccagggctcg gggccctgcg
     7081 ccatggctgg ctgacggcgc atcgtggccg cctgggaggg cggcacaccg ggcaacaggc
     7141 cgcgttcggc gacgacgtgc gccaccgggc gaggcagcag gcgctcggtg aactcctgcc
     7201 aggacaggcg tggctggtga acgggcggat cctcgccgaa acggatgtcg aagctgcggc
     7261 ccagggcgtg caccgaggcg atgccgccgg tgggcggccc ccacaggtcc agccccaccc
     7321 cgaagctccc ccggagtgtg gcgacccaca ggtccacctc tacccacaca tcgacgtgga
     7381 tgctgacccg gaagtagaag gggtccctct gcaccatcgc gtccagtgag gcgtgcaggc
     7441 ccgcgctgat cggcccggcc tcgaaggaga cgtccagccg gccgccgacg cggaacgagg
     7501 agggcaccag agcggcgtag caggaaccgc gcaccgagat gtggtcggac accgaccagg
     7561 acaggccgat gggctgcacg tccggatagt ggtcgggttt gtcgtagccg ggcccgtaac
     7621 cgccggcggc caggacgaag tcccccgggt gggcgctgtt gccgaaccac aggcgcaagg
     7681 cggcactgcc ggtgagatgg caggagtggt ggaccaggaa cgacgcggga tcgatggcgg
     7741 cgtagatctc cagcgtgtcc tcgctggagg tgtacgaagc catcacgtcc agcgccacgt
     7801 tggcgtaggc ggtggcgggc gcgaccggga actgggcgcc gacctggccc agcagcgtgg
     7861 ccgtgaaatc ggtcctgccg gtcacggcga actgcaccag ggcgagcgca cggccgtgca
     7921 agaactcgaa cgagtcgaac tgcagccccg cggccagcca catccgtccc ggggtccgcg
     7981 tcacccacgg tgtgctgccg ccggtgagct tctccaggta ctccagcgga tccgtggtga
     8041 cgccctgggc attgccgggc atgaacggga acttctcgac gtcccccgcc tccggtacgc
     8101 gtacggcgct gttgtagccg aacccggcgg cggccccggt gatacggaag gggggcggcc
     8161 cgatgccctc gccctccttg aacgcgagct gtccgtagac gaacaccgag ggttcgcccg
     8221 tcagcggctg ggcgtagccg ccgaccgcac ccagcgacag cgccggggtg atgacagccg
     8281 ccgcgccctc gacgtcgatg aggtaattcg gggtgtggcg gttgaggagt gccccggaga
     8341 tcttgatcgg ggaccggtcc caggccagtc cgagaccgtc cagtcgggcc tcgaccgtgg
     8401 gcggctgggt gaggccgatc ccgagtccca tcccctgggc acttatggtc agggcgtcgc
     8461 cgctgatccc ggcgtccagc agcagccaca cccggtggtc ggagtactgc acccccagcc
     8521 ggtccagccg caccggcccc aggctccgcc ccaccgcata ccaggtgatg gcggtgtcgt
     8581 tcccaagtcc ggtcctggcc gctgtgccct tgccgccggt cgcggtcagg atgaggtctc
     8641 tgtccccgac gtggacgtcg acacccagct gtagcgttcc cgtcaagccc tcggcgggaa
     8701 ccctgggacc tgtacccgag tggtcgaccg cgccgagcag cgcgttgacc ttcgccgcct
     8761 cgtccgggcc tagtccccgg ttcgccgccg ccgcgtacag gccccgcagc cgcaccgcgt
     8821 ccggcggcac atgcggcccg atcaccggca ggttcgacaa ccccccggag acctcggccc
     8881 ccaccagcag aacccgcacg ggctttcccg cgaccgaatc gccgctggat ccgtgcgcgg
     8941 tccccggggc agtggtccgg gatgccggcg tgcccggccg ctgaccgtcg ctcatcgcga
     9001 atgccggatg gggcgcgata atttggctaa tgacatgggt gtgcccgtcc attcggtctg
     9061 aactgcgggg gtcgcctctg ccatggtcgc gggcacgtgg cggggagttc cgatgacggg
     9121 gctgtgacgc cgccccgatc gctcccacca tagggtccgc atgatccgtt cgacgttggc
     9181 ggtcagggga gttcacccgg acgtattaac cccgccctgc cccggcggct ctcgatgggg
     9241 gcagccgata tcgagcgtga ggcgcacccg cgcggtgccg gtcatcaggg cgtgggacga
     9301 agggcatgga ctaccgccgg acatccccgg tcgtgcgtcc tggagcgcga aaccggatgg
     9361 aggcgcgcag gcggcagagc ctcggccctg ctccgcatcc atacagctac agatcgggaa
     9421 gcggccgctt gtctccccgc gaaaccggtc ccggccccgc ttgcttccgc gagctgggcc
     9481 cctttggtga tggccgcgaa acaggtgagg cgtagtccgc cgagttcggc gaaccgcaac
     9541 tggacgccag ggtcggatag ttcctcgcgg acgatgagcc gcatgccctt cggcatcggg
     9601 ccggcacgcg gggaatcggc tccggctggt gtgtcgtagc cctgcaacag ggaccgcgag
     9661 tccttggagg cccacccgtt cgtgtgatcg actgctcagt gcccgagatt gacgcgaagc
     9721 gcaacgcgac gcttcgccac cggtcggaca cgactccggt cgactgcggg gccggttcga
     9781 tccagtcgcg ctccgcgggc agcgggcccg tgcgccgaag aggcgcgcac gtacctgcgt
     9841 acgagccggc gcacctcaaa caggtggaca gaccgggaag atcacagtgg cggtgtcagg
     9901 cgttgtgggg tccgcgcctt cgtacgcact ggctcctgcg gaaacgtcgg aatccgagcc
     9961 cgctgcgcac tcgtcagtcc ggtcagaagt gcacactcct ctaggtgccg tggaggcgtt
    10021 ggtgaaggtg tcccagtgcc aggagcgcga agtggtcggc gaggcggggg acgttgtaga
    10081 ggaacgggcg tgttccgagc atgtcgggtc tggacgcgat ggagccgtcg tcgcgttgcg
    10141 tgtccaggag gaacgtcgcc gcttcgatcg ccgggcgggg ggcgtggtgt ctgcacaggc
    10201 acagtaaggc gtaggcggtg ctgatggcgt cgctgcattc acctggttgc tggccccatc
    10261 ctccgtcgga gttgcggtgg tgcagtacgg cggtgagcag ggtgcggacc attcggcggt
    10321 cggtaccgtg aggtgcgggg cgctggtacg gggcatcggc ggcgaggagg gcgcggaagg
    10381 caacgtggaa gcggctgcgg ctccattcgg gcgggaagag cgcgtcgggg cgctgggcgc
    10441 gggtgaggaa gtcgagggcg gcgtggatat ggggccggtg ttgggagggc cggatgctga
    10501 gggcgttgac ggccgcggcg gtcatgccgg cttccgagcg ggcgcccgcc acgtaggtcg
    10561 ggaagccgcc gtcggggccc tggagcgagc gcaggtgtgc gaggccgcgg tcgatggggg
    10621 tgcggtagcg ggtggggtcg agggtgtgca ggaattcgac agcgacggcg gtgtcgtcgg
    10681 tgtcggtgtg gtgggcgtgg tcggtgtagg accatccgcc gtcggcgcgc tgctgggccg
    10741 ccaggtggcg ggcgaggcgg tgctgcacag cggtgggcgc gccgcaggag gccagggcga
    10801 tgccggcggt gacggtgcac caggtgtcca cgccggtcac gaacgggacg ccgccgtcgg
    10861 gacggcggca cgccagggcc tggcggatcc cgccggccac ggcggcttcg tggccgggca
    10921 gcagggacag ggcgttggcc gccaggaggt ggaccagcag gttcccctcc cacacccgcg
    10981 gtgccgaggt ggcgggcagg agttcgtgtg cggcggcggg agcgggccgc ccggcggcgt
    11041 gggccaggat cacgcggata gcgatcaact gtacgcgtcc agccgggtgc atcccttggg
    11101 ccgggatggg tggcggtgcg acatggggtt gtggccggcc gagcaggcag agtgtcgccg
    11161 cgatgagggc gtgtttgcgg gttctcatga actcgggtgc ctcgtccacc agtgcgcgga
    11221 tcttgacgtc gtcctcgcgg cgcaggtttc ccgtcgcggc gcgggccagg acgtcgtcga
    11281 tccggtcgcc tcccgtggtg cgggcggaca ggaactgttc aaggtgccgg gccgcttggg
    11341 gggtggtccg ggtgcggcgc agcagggcca gcaggagggc ggattccagg acgcggctct
    11401 cacagtggcc gcgtacggcg ccggaggggg tgacggaatc catcacacgt gtgcgcagga
    11461 ggtccacacg tcgggtgtgg tcgtggggca gcgtggtggg cggccaggga cgggtgcggg
    11521 tggggcgggc acgcaacggg gatcgtcctc ctggcccgta tccagatcgg gttgggcggg
    11581 cgccggccgc cagtccgtct tgcgggaggc cggcgttccg gggtgtgtgc tggccggtgt
    11641 gtgacaccgg atccgtggtt cggccgtgcg tcgcggaccc ggagcccagc cgacggaggg
    11701 cgacggccgg gaggagcggc ctcccggccg tctgcgcgtg ggtcgaccga ggccggggcg
    11761 gggatggttc gttgcccgac ggtggtggtc aggcgccgaa gccggcttcc ctcaggcggg
    11821 gccaggcgtc cgacacccga accggtccgc ctgcgaccgc gtcgaggctg gcgtcgtact
    11881 tgaggtagga gccgttcttg aagaagtaga tgcgggagtc cccggagtcg tcggggtcgc
    11941 gttcggccac cgctcccaga tctgcggcaa agtcggtgcc cgcgagcccg gggaagcact
    12001 cggcaatggg ctgcgggccg acctcgacgc tcttgtcgcg caggttcagc gtcagacagc
    12061 gagatccctt aaacaaccgg acgtggtcgg tgtccagacg catcgcggcg tccacatccc
    12121 gatcaaaacc agaccgggtc agggccgacc aagcgctgcc gatggcgctc gggcccgcgg
    12181 tcagggtgtc gtcgtccagg ttgtagcgca cgaacacctc acccctgccc aggtacgcct
    12241 cgttgggggc cccaggtgtg ggggacttgc tcgcggccgc gtccactccg tgggcgaaca
    12301 ccgtccccgc gagagcgggc agaccctcca cgattgggcg gggttcggag tgcgcgtggg
    12361 ggtcggaggg gtcgaacacc acataccgcg agccgctgaa ggcgtacacg cgggtgggat
    12421 ccagacgcag agcggcatca atcacagaaa cactccgggg gttcgggcag gccaggacgg
    12481 ccacgccgtc gaatagaagg caggtaccgg ggggtcaccg cataggcgcg gcgcggagcg
    12541 cgacggcgtc agaacacggc acagagttat ccgacaccga ggcgttgttc atcgaacgga
    12601 cacctcgttc ctatcggacc ggtctccttg gtcagggtgc cgctcgtgac cggatcactc
    12661 ctatggccca acaacttcgc cccccagagt tcggccagcc cgtcgcgacc cgaccccgct
    12721 catccccgac ccccgaatcg ccggacacgc ccacatgtac gaagggccgg gcgccgcctc
    12781 acttgcgacc tacgccgccg cctacagccc gaaggagacg atcatcgatt tcacggaacc
    12841 ggcagcggtc atctgccgga aggtggcagc tctcactgtc gcggttccgt gggctcagat
    12901 gtccgtgcgt ggttgcgagg ctgtgatcgt gaaagccgag cttgccgagt ccgacggcgc
    12961 cgctgttccg gggactctac tggcctcgca cagcgacggg tggacggttc aggcggctga
    13021 cggtgccgtg cgactcactg gaacacggtc gcgagccagg aatcggacca cgtcacgccc
    13081 tggctgcggc ccttctgtcc actgaagcag gcacctcgcc ccggccttct tccccggcgt
    13141 gccgggcata gagatgatcc cggggaactt cggtatcagt ggccgttcgc gccggctcca
    13201 cccgagagcc gacagccgca cacgccgttg tcctagtgtc ccagggtttc cgcgctgtca
    13261 tgaacgctcc agggcgttgg ggtgattgag cggagcgtga ccgtgaccgt gacctgtggg
    13321 gtgtgcgcac gttgacggga caggtcctga tcccacctgg gtcttcacca tcatccgtct
    13381 cgtgctccgg gagttgtgct gtgaacaagt cgtctctgcg tctggcggcg ctcatggttg
    13441 gttcgctggc ggtccttgcg gcggtcgtgc cgccggtgtc ggccggagaa gcgggtggcg
    13501 tcctgccgcc gctgccgttg aaggccggtg acccgtacta cttctgcctg accgacgcca
    13561 cggagacagc cctggccaaa gcccggatca gtctgcaggc ggtctccccg aacacggtgg
    13621 tgacctacaa ggcccacacc tgcatgcgcg gcactctcac gagtggacag atcaacacgg
    13681 acctcaccgg gttgagtggt gagggcaccg gtgggttcgc gttccagcgc gacgccgagc
    13741 gggccgagtt cgtccacccc cgggtcacgc tgagccccga catgaccggc tcgtggaccg
    13801 ccgaacacgc ggggcggcgc atcgagatgt tcacctccac caaggtcggc gcgaagttct
    13861 cgctcacgaa ggtgagcgca accgacctgc cgatgaccct gacgaagcag ggcgccgacg
    13921 tgctggccga cacgttcggc accagccccc tgccggcggg gaccccgttc ttcgaaggca
    13981 acgccagctt cgacgttctg aactccctca ccgcccccct caccacgaac ccgtaaagct
    14041 gcacggcccg aaggaccgtc ccagcaacgt agagacgcga agcgccatgc gccgggagag
    14101 ggcgctatgc acgtgtggcc gagcggtcgg tccacgagag aggatgcaga tgatggtgga
    14161 gcggctgctg gcgccgcacg aggaggccat tgacgcggcg gggatccggc tcgtgctgca
    14221 caccgagctc gacggcgacc tggacgaggc gaccctggcg caggcactgg ttcatctgca
    14281 tgagtgttat ccgctgtccg ccgggaggat caccacccgg gccggggagg gccgagcgat
    14341 cgtacgggtc gaggagcggg ctgcggcgcc cgtactgggc cgtggcagcg atatccgcga
    14401 ggagatcagt gccccgctcg actgggagca gggcccgctt ctgcgggtct cggtcgtacg
    14461 cgacggccgt acccgggtgg tgatgacgtt gccgcgggct ttcgccgatg ctatgagcta
    14521 tcaggcactc catcagcgtc tgtggaccat ctacaccgca ctggccacgg gccgacccgt
    14581 gccggccgag ccggtgcggc cggcactggg cccggcgctc gacgacgtgc tcgccgcccg
    14641 cttcacaccc tcgaagctgc gtgacttcgt cgccgaacgg gcacggctgg atactcaggc
    14701 gccgcccgcg ctcctgcccg ccctcgcttc ccagggcggc gggcccggta ccgatctgaa
    14761 cttcggaatc accagtgtcg aagccggccc cgacctgtgc gagaagctcc tgcagcgggc
    14821 ccggaatgca tcgctcagcc tcaactccct ggtgagcgga gcactgctga ccagcctacg
    14881 aactctgttc cccactcacg gggaaccggt gcgaatgctg tgcaccatgg ccgtcgacat
    14941 gcgccgcaga ctcaccccgc ccatgccggc cgagatcctc caatcagcag ccaccaccac
    15001 atccatgcgc ctgtggatcg acagccgcgc acggcccgaa gacgtgggcc gcaacgtggc
    15061 agctcaactg cgtgccgatc tggacagcgg ggccgcggcc atggaactgg cggcattccc
    15121 ctacatgctc gaccagcacc caccaagcct ggtcatcacc aatgtaggca ccatcgccga
    15181 accacccctc ccgcaaggac tgcggatcag cagggtgcgc ctggccccgc tcggacacgt
    15241 acccatgatc ttcgccgtga tcagccgcta ccagggccgc ctcaccatcg acctgaccca
    15301 cagcagagcc tggtacaccg aaacccagat caaggaactc agcggacacc tgtccgcgac
    15361 tctcgacgcg acctgactga ggtgccgcgg agtttccgga caggtccccc aactatgatt
    15421 gggggccgtc tggagaggtg gaacaattgt cgcgtaggaa tgggcgttgt tcgccggagt
    15481 tgaagcagga atctgtccgt atggtggtgg atggtccccg tccgattgcc caggtggcca
    15541 gggaactgga catcaatgag accacccggg gaatcgggtg gccgcccacc ggtgcagtag
    15601 cgacgggaac gatcccaccc cggcatcggg ggacaggaca gagcgggaaa tccagctgga
    15661 acgcgagaac cggaaactcc gcgaggaaaa cgatttcccg aaaaagcagc aggcttcttc
    15721 gcggcagaca agcgataaca gccaaatacg agctgatcga cgcggagaag gaccaccacc
    15781 acatcgtcga cctgtgcacc tggctgacgg tgtcccgttc cgaacgagtc agcaccgaga
    15841 cggtccgcca cctgatgcgc gacctcgacc tgaccccctg ccagcccagg ccctggcggc
    15901 cagcccccac cgaccccgac cgccgccacc ggctacccga cctgatccgc cgcgacttcc
    15961 tcgccaccac ccccggcacc aaacttgtcg gcgacatcac ctacatcccc acctgggaag
    16021 gcttcctcta cctggccacc gtgatcgact gctgcaccaa aaaagtcatc gcctgggcca
    16081 tggccgacca ctaccagacc ccgctccgct catcgaagcc gccctcacca tggcagccct
    16141 caacaccccc ctcacccccg gagcgatctt ccactccgac cgaggatcga actacacctc
    16201 cgacgccttc gcacggaaac tcaaaagcct caacctgcgc cagtccgtcg gccgcaccgg
    16261 cgtctgctgg gaaaacgcca tggcagaatc gttcttcagc accctcaaga acgaatggct
    16321 caaccgcttc gaattcacta cccgcagcaa agcacgcacc cgggtcgtca gatacattga
    16381 gggcttctac aaccccaaac gcctacactc cgccctcggc taccgagcac cccaagaaat
    16441 agaagacgaa caccaacaac ccccactcgt cgcataaaaa acaaacaaac cactgtccgg
    16501 aaaacgcaag gcccctcaaa cgaacccgtt gctgtcgttc cccacaccaa caggtgttgc
    16561 ctgcggcggc ttgtgccgca gaagcgtatc cgtacccggt tcgcgcccct gtcccgtgtt
    16621 ctttgtgtgc tggccgcgag gacgtgcgtc agcagtgaat ccgccgacgg actcccccac
    16681 gggccgcaac caacgggttc cgtgatcgtt ttcctgttgg gcttaggggt ccttgcaata
    16741 ggggagtccg agccgcgggg gccggcaccg cacctcgcgg tgttgccgaa aagccctagt
    16801 agtgcttggt catgttggtg cggggtctgg catgtcgcgg tgacaggtgg ggcaggcgcc
    16861 ggtccaggtc gcgaggagtg tctgcagctc gcggacgact cggtagaggc tcaggccgac
    16921 gccgtctctt ttggggatct gttcagtcgc tgaagggtgc agaaggcgtg ggcgaccgag
    16981 acgagggtga cgtggtggtg ccagcctggc caggttcggc cttcgaagtg ggccaggccc
    17041 agggcctgtt tcatctcgcg gtagtcgttc tcgatgcgcc agcggagctt cgcggtgcgc
    17101 acgaggacgg gcagcggggt ggtctcgggc aggttggaga gccagaactg cacgggcgcg
    17161 tcctggtcgg cgggccattc ggccagcagc cagcggaccg gaaactcgct gcctggtgtg
    17221 gctttgcgga tctcacgtcc ggcgggccgg atccgcaggg ccacgaagcg cgagtacatg
    17281 cgcttgaacc cgctgcgtcc gctgcccggc cgcgatcctt ccctccactg caccggccgt
    17341 gcggaggatt tgccggccgc gatgaccagg ctcttcacct gctgggccgg ctcggggtag
    17401 gcaggaaccg gtcgtgggcc ccggccggag taggccgggg tgcatggctg tgcgtcttcg
    17461 ggttgcgcgg tggtcgtggt cgagatgccc accacgtagt cgaggccgcg ttcttccacg
    17521 ccgatccgga aagcggcggt gtccccgtag ccgccgtcgg cgatgacctg gggtacctcg
    17581 atgccccagg accgcgtctc gtcgatcatg tcgagggcca gctgccactt ctcaacatgg
    17641 cccacctggg caggaatggc acatttgtca cggcgggcca ctgtggcccg gtcggccttc
    17701 ggcgaggcgg gatcccaact accgggcagg aacagacgcc agttgacggc cgcggaggca
    17761 ccgttggaag ccaggtgcag cgagactccg gcctggcagt tggtgacctt gcccgcggtg
    17821 ccggtgtact gccgggtcac gcacgccgac gcgtccccgt ccttgaggaa cccggtgtca
    17881 tcgatgacca gcgcggtcgg tttgatgacc ggctgcatgt gccaggccag ccgggcccgc
    17941 acatgcgccg catcccacgg gctggaggtg acgaagtggg ccagcgcctg ccggttcccg
    18001 tcctcgccca ggcgggcagc catcggctcc accgacttac gcccgccgtc cagcagcaga
    18061 ccccgcagat agaccccgcc ccaccggcgc tgatccgccc gcgcgaacgg ctcgaacatc
    18121 tccgccgcga agtcctccag atcacaccgg accgcagcca actcctcact cagcacacac
    18181 gatcaacgac acgaccgatc aagaagacac gccaccgcag accgcacatg accaagccct
    18241 actagtagct ccgctacgag ggctctccgg cgccttgcga cgcacggcac cagcccccgc
    18301 ggcctgatcg gactccccta ttgcaaggac ccctaaggtg cgcgtcagtc gtcgaaggtg
    18361 ttggtgcagg cggcgtcggc ggaggggttc agggccccgc cggcgtccac gttgaagccg
    18421 caggcaccta ccgggaggcc cacgggcgcg ccggtagcat tcccggagat gacgccggga
    18481 gagccgaccg ccgcgccgat ggcgtcggag tcggcggagg cgataccggc gctgccgagg
    18541 aggacagcgg cgctcaccgc gaggacggcg atggtcttct tcacaggaat ctccttcaag
    18601 acgtggaaac gggcggccga tcgaacccgc acagcccggc acggatctga ggcgcaggcg
    18661 cctgggcgtc cgtgcccttc caacgggccg accgggccgg tggacacggc cgccgccccc
    18721 cctacgggcg ccgcgttcac accgacggcc cctcgcgcgg gagcggccac gccgagaaca
    18781 ccgccccgcc ggccgtgagc ttgactccgt cggcggccgt gtgagcgtca gccggtgacg
    18841 tgtaggtggc tggctgctcg ccgagagcgg gttcggtagg cgatgtgagt gacgttgcag
    18901 gccaggctgg ttggtacgtg ctgggcgtgc ttttttggcg ggcaccggta cgggttttgc
    18961 gcaggtcgaa ggtacggacg tcccggcgag ggtgacttcg gaaccggcgc ggcggatcga
    19021 gcagcgcggc tggccgtcct gggtgtgccg gcccaggtgg ttgtgctgct ggatctcgtg
    19081 cagttcacgg gccacacggt ccggaccatg tggcaagggg gatgtcggac ctgcccggcc
    19141 ggcggtgttg cgccgcctcg ttcgggtgaa caccgcgcgg ccggaccccc ggaccgggtg
    19201 cgacacgtgt cggatgccgt gggggagcag ggcccgctcc cctcctcttc ccgcccgccc
    19261 ggtgcgggcc cgagatgaac aggacacacg cccgatgcac cgttccagaa gtccgtgggc
    19321 actcaccggc cccgccgcca cgctcaccgc cgtgctcgtc gcggtcgcgt tgccccccgc
    19381 ccacgcggtc accgccgccc ccgctcacac accggccgcg gtctcccacg cgccgcgagc
    19441 cgtgatgatc ccgccgcagc aggatgtacc cgaactcaac gcgccgggcg acccggtgga
    19501 cgtcgtcttc ccgcccgaca accggacccg tccgggcacc ggtcgcttca ccgtcaccgc
    19561 accgccccac acccgcatca ccgcactcgg catgcgctgc ctgaccaaca gctcctgctc
    19621 cgcggcgatc gccggtgacg gctccaccgc caccgtcacc ctgcgccggg ggcgctggac
    19681 cttctccagc cccgtcaccg tcactctcca ggcatcaccc agcgcgccac tgcccggcgg
    19741 ccgctacgcc ggcgtcttca ccgccggcaa cgagtcccag cctttgacgg ttgagatcac
    19801 cgagggtaac cagggcaccc tgggaatctc ccacaaggac gcccccgacc gtggcggagc
    19861 ccaggtcgtc ttcgtcggcc gcggcggggc cgccgacacc gccggcatcc gcgagggcga
    19921 catcatcacc agcatcaaca acaccccggt gaccacctcg aacgagttgc gcaacgcacg
    19981 gctgggcaaa ctccgccaag gcgccaccat ccccgtcacc taccgccagc ccaacggcac
    20041 cacccgcacc acccaggtca cccttacctg aaccacaccc ggcgccggcc gggtcccgcc
    20101 gcacccgaac ccggacggcc gtcacggcat gtgccgggtt cgggtccagg cgccccgcgg
    20161 cgatgccctt cttcgcctca caggcgaaga acaccgacgt gtgcgcccag catcaggccg
    20221 agcagaactc atcgacggga tcaccgccga gctgaccgtc ctcgccggct tcgaccagga
    20281 cctcgccgcc gaggccaccc gcaccagcaa ccggatacgc ggcctgctca cccagttccc
    20341 ccccagcctg gaacgcgtcc tcggaccccg gctcgaccac cgggccgtca cctggctcct
    20401 cgaacgccac ggctccccgg ccgccctgcg caaagccgga cgccgccgcc tcgtcacact
    20461 catccgcccc aaagcccccc gcatggccac ccggctcatc gacgacatct tcgacgccct
    20521 cgacgagcag accgtcaccg tccccggcac cggcaccctc gacaccgtga tcccctccct
    20581 ggcccgctcc ctcgccgccg tccatgaaca gcgccgggcc ctggaaaccc ggatcagcgc
    20641 cctgctggag gcccaccctc tttccccggt cctgacctcg atgcccgggg ccggcgtcag
    20701 gaccgccgcc gtcctgctga ccaccgtcgg cgacggcacc agcttcccca ccgccgccca
    20761 cttcgcctcc tacgccgacc tcgccccgac aacgaagtcg tcgggaacct cgatccacgg
    20821 cgaacacgca ccccgaagcg gcaaccggca gctgaagcgc accatgttcc tctccgcctt
    20881 cgcctgctga acgccgatcc cgcctccgcg cctactacga aaaacaccgc accagcagca
    20941 aaacccacac ccaggcactc ctccgcctcg cccgccaacg catcaacgtc ctcttcgcca
    21001 tgctccgcga cggcacctaa tacgaacccc gaacccccac cgtcatcctc gccgcataac
    21061 cagaccgaac cccccacaac cggcaccaca gaccttgacg aaagacatag aggcaccccc
    21121 cttcagggcc tctatgttca ccgagccggg ggaggtaggg cgtcttcctc tttcgggtgg
    21181 agcccggccc aacccgttga tcacccaggg gatccggttc cacactttgc gcaactgagt
    21241 attcgtcggg cttcgctggg agaaatcatg ctcaaagtca aacgggcgca cgtgaccgtc
    21301 gcagcctccg tcgccctcgc gttggccgcg ggaggtgcga tcgctaccgc cacagagagg
    21361 ggaagccggg cacctgccgc cgtgccggtc gcggtggccc cctatgcacg ggccgccggg
    21421 atcgtggaca gcgcggggaa catgagccgg gcgaagggga tttcgtcggt caagcgggtc
    21481 tcgaagggcc ggtactgcgt gaaggtctcc gcttccggct tcgacgtcag aacggc
//
