LOCUS       NZ_CP045178            10978 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4909725
            Orig. end                         :: 4920703
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10978
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="21"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10978
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10978
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5978](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5978)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(252..266)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.44"
                     /tool="antismash"
     gene            complement(1081..2085)
                     /locus_tag="GBW32_RS20615"
                     /old_locus_tag="GBW32_20605"
     CDS             complement(1081..2085)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="GBW32_RS20615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20605"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077970715.1"
                     /transl_table=11
                     /translation="MNATSIGPQALRLFGYASARSDWTVRSACEQLDTTADEIERARKE
                     LLVLGLLIEDSGEDSADGAPTVKATAPEAALLRLLSAEEEAAARLTDQIRHTREQIDAM
                     REVFLPLHTARTSELSGEVVSSPEKIARVLSDTADIATSEILSMHPGPVMAPEVLAQGL
                     RRDVRVLERGVVMRSIHLRPMTRIIHSMTHLQELGAAGAQVRIASIVPFRFILVDRVQA
                     LVPALHTNSSNAMMVLRGEAVTALLVKVFEMCWASAAPLETAETGQEAGFSSQQLWVLR
                     LMATGRKDEAIARELGVSVRTLRRVMAELMARLGVESRFQAGITATKMGLLDH"
     gene            complement(2144..2710)
                     /locus_tag="GBW32_RS20620"
                     /old_locus_tag="GBW32_20610"
     CDS             complement(2144..2710)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.5"
                     /locus_tag="GBW32_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20610"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_077970713.1"
                     /transl_table=11
                     /translation="MSQERSVRTRALLLHAAAEEFAARGYSGARLATVVEHIGMTKGAL
                     YGHFSSKDELAAAVAHEATLAWETLRAELAAECLDPYKELRLLAEQLARLMDDDPLFGA
                     AVRLASDGFHGCVEQDLPSSVAAHITRLVLRARQEGEPAQAFPSAIFADLVTAAIFAGT
                     RSGLEPAGGSRTRLNGLWDLLGVGS"
     misc_feature    complement(2760..2776)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    2787..2801
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.57"
                     /tool="antismash"
     gene            complement(2849..4279)
                     /locus_tag="GBW32_RS20625"
                     /old_locus_tag="GBW32_20615"
     CDS             complement(2849..4279)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="GBW32_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20615"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_193385998.1"
                     /transl_table=11
                     /translation="MLGACEGVLRTWWDRKAEWLDPAGGLTPRQASDQRVLATASAHIQ
                     AARLLLERAAQRADHAPVTPVLVAENQRDVTAAVELCCSATDELFRSSGIRVLDEGDPL
                     QRAWRDITAARSHGTLNFDTAAASYTSALVAARGAEPGRGTAADLAGAPPPSTRWPRPR
                     PAPSPRDRRPPRPGAPGQRGGGRRPGTGRRHGGGTVTRRIALFDVDQTLLAEKSMISFW
                     RFWSASLARSGRRVPAPRTSGADRETLNRAYFTLFAGVRRRRLEEAAAEWYAHYRRGPD
                     AYLTATVAALEEHRASGDEVALVTGCGDILVRPVAGHLGVLHVLATEQLTDGSGVLTGE
                     VRRPMIAAAKAEAVVELARRLDVPLRECRAYGDHSSDLAMLSLVGHPVVVGDDPVLAER
                     GAEPGWRFLSATTGPQPTEAATTTATTEAATATGKQQQQQATATGAQHMASGTALRHVD
                     TADRRQHVGTVTGLVHAT"
     gene            complement(4294..5007)
                     /locus_tag="GBW32_RS35815"
     CDS             complement(4294..5007)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS35815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_193385999.1"
                     /transl_table=11
                     /translation="MLTDLEQTRRFAREDRARLDEECALTDRLAESLTTDGFGRHFVPE
                     RWGGCAGTFTALLDAVSVLSETCASTAWCGALYASHSRLASYLPEAGQRELWGGTLTCG
                     SPPRSYHRRAARNARATAAVDRPVAAASGVRQAEWILLASGRRTRTATRGGRVPPPGRV
                     ARRPGAGSTGSSVPRDQVRVLDNWHPSACAAPAAAPSRPMSSSPATSPSPSATWAGSRR
                     TGRAVTRFPSPWSPP"
     gene            complement(5001..5978)
                     /locus_tag="GBW32_RS20630"
                     /old_locus_tag="GBW32_20620"
     CDS             complement(5001..5978)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="GBW32_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20620"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_193386000.1"
                     /sec_met_domain="AfsA (E-value: 1.8e-20, bitscore: 71.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYETDMETGTTIAGHRADAHVPRTLVHKAADSEVLLSSVRPLADD
                     RYAVWVRWPHDHLLYRRGTDGTRDSLVVVETLRQAGIYLSHRFHDVPMDHAFVLHGFSF
                     QLDTPIRDTGDGSDVVFEITVCREAGNPRRFRMSCEARIRVGGRPAGRATMRWDAVAPR
                     QYQTLRSRGIPVPRAAASAVHPPRPLTPQAVGRDREDAVVLADSPETDGGWQLVMDNRH
                     PALFDHASDHVAGMVIVEAFRQAALVRTGHATPGSWAVTGIQMGFESFGELDARAVITA
                     EPEGPDTFRLTARQGPRTLPTARVTGSSTSPLGKLIAQAGVATC"
     misc_feature    complement(5748..5750)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6073..6093
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.22"
                     /tool="antismash"
     gene            6172..6858
                     /locus_tag="GBW32_RS20635"
                     /old_locus_tag="GBW32_20625"
     CDS             6172..6858
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039544.1"
                     /locus_tag="GBW32_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20625"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_077970702.1"
                     /transl_table=11
                     /translation="MKAGTIVEYKQDRAVFPVPERAEFTAGQQAARSPELHQERAVQTR
                     RQILESAAILFDQNGYAGTSVKDVADHVGMTKGAVYFHYPSKEALTVAVVQAHYDRWPR
                     LLEAVQAEGLGPMDTLVELLDRTAMTFRDDAVVQGGARLMMEQPLVEGSLPQPYVGWVD
                     LIELLLTQAMDAGELREGVSPTAAAHTLVAAFFGTQHISARLDLRVDLVKRWEEARDLL
                     SYALRA"
     gene            7086..7526
                     /locus_tag="GBW32_RS20640"
                     /old_locus_tag="GBW32_20630"
     CDS             7086..7526
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132275.1"
                     /locus_tag="GBW32_RS20640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20630"
                     /product="DUF3592 domain-containing protein"
                     /protein_id="WP_077970700.1"
                     /transl_table=11
                     /translation="MKILFLAIPALIACVALGMAVSAMRERLRLRAAWRGGLTAEAHCR
                     RTYATTGRRGGSGTSTTLHHVYEFTARDGRTVRFEEEDGPATTLEGDIVVVSYAADHPE
                     RATAQEPGTALRHTAHTVGLLLFCAVMVAFCVFFATSALRSL"
     gene            complement(7638..7826)
                     /locus_tag="GBW32_RS20645"
                     /old_locus_tag="GBW32_20635"
     CDS             complement(7638..7826)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021464.5"
                     /locus_tag="GBW32_RS20645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20635"
                     /product="DUF2180 family protein"
                     /protein_id="WP_077970697.1"
                     /transl_table=11
                     /translation="MLCYECALDDRRQASAAVCTRCGIAVCTEHSAVTTVRPRRAIGLG
                     TTTLGSPARLATCTTCR"
     misc_feature    7877..7890
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.42"
                     /tool="antismash"
     misc_feature    complement(7904..7913)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.58"
                     /tool="antismash"
     gene            7985..8470
                     /locus_tag="GBW32_RS20650"
                     /old_locus_tag="GBW32_20640"
     CDS             7985..8470
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1266: hydrogen
                     peroxide sensitive repressor"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547377.1"
                     /locus_tag="GBW32_RS20650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20640"
                     /product="Fur family transcriptional regulator"
                     /protein_id="WP_077970695.1"
                     /transl_table=11
                     /translation="MDAFAEELRAKGLRSTAQRRAVLTALEGAPHSTAPGLESALGVGR
                     PGAAPERTGDGSGRTGDAPAFSRQGLYNVLDDLTRAGLVRRIEPAGSAARYELRVGDNH
                     HHVVCRSCGDVEDVDCVIGAAPCLEPLEDRGFVIEEAEVTWWGVCGPCRAAEPARGG"
     gene            complement(8740..8877)
                     /locus_tag="GBW32_RS35820"
     CDS             complement(8740..8877)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS35820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_179120256.1"
                     /transl_table=11
                     /translation="MPGAPLDPTDASELHGYTLRRRLGSGGMGVIDAGTAEGRPYLVTE
                     "
     misc_feature    complement(8836..8838)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9045..9055)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(9122..9793)
                     /locus_tag="GBW32_RS20655"
                     /old_locus_tag="GBW32_20645"
     CDS             complement(9122..9793)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032651.1"
                     /locus_tag="GBW32_RS20655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_20645"
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="WP_077970693.1"
                     /transl_table=11
                     /translation="MTTTQFWGDYAQTKPSPRETNAAGERTWFNWTQYPEHGPDEEILR
                     RQGFGMPERALELGCGSGRNIAHLATRGVTSVGLDLSDVQISRARVKWGDVERLHFVHT
                     EATEFLRRAEDRFDAVFSIFGGLWFTDPAVLLPLVHERLNAGGVLAFSHAPAIEGCYGP
                     QGAYKGAFAGDPPILRRWAYTPQMWTGILLNYGFMDVSAVVLSAPHAGDLGTLLVRAVR
                     I"
     gene            complement(9803..10261)
                     /locus_tag="GBW32_RS20660"
                     /old_locus_tag="GBW32_20650"
     CDS             complement(9803..10261)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS20660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_20650"
                     /product="hypothetical protein"
                     /protein_id="WP_218670023.1"
                     /transl_table=11
                     /translation="MPTTDPTTPIKKVDPREIMGEHLLDRLATRITADHPHMGPEYARR
                     IMAETVKFLVAGVECDEPLSPSETVDVGWHTFILHTADYAEFCDRVAGYFIHHNPEYLD
                     EKTHGGAKNVRQRTLDAITAAGFEADLPLWPEVADCHQCHAGCHDSPK"
     gene            complement(10373..10537)
                     /locus_tag="GBW32_RS35825"
     CDS             complement(10373..10537)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS35825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_179120255.1"
                     /transl_table=11
                     /translation="MTVQAEEPQANAEESPVFIEGEEIPKCPGCRCLVIWEDTTGECLH
                     CGAPLKPDA"
ORIGIN
        1 accggagtcc ccgtacggca gctccgtgat gatcgggaag gggcgccgca gcgcggtgag
       61 cgcccccgac tccccctcgg gaagcggctg ttcgagcatg ctcacctcgt aggcgcgcag
      121 gacgggcagc atccggtgca gccggtccac gtcccacgcc ccgtccgcgt cggccatcag
      181 ccaggcgtcg ggccggtagc ggcgcaccac ttcgagccgg gcgaggtcgt cctcgacggc
      241 gccggctccg agtttcagct tgagaagcgg aaacgccgaa ctggcgcgca actccgcttc
      301 cagtgagggg agttcgaggc ggtcgacggt acgtacgacg gggacgggtc gcggcggcgg
      361 caccccgagc agctcccagg cgggacggcc gctgcgcttg gcgagcaggt cccacagcgc
      421 ggtgtccagg gcgctgcgca ggggcccggc gggcagcagg tccggcagat ccaccgggct
      481 gatcatccgc tcaagtccgg ccgctgcacc ggcgatctgc gcgcgcagga cagccgggtg
      541 gtgcgcctcg atgtgcggta ggagggtcgc ctcgccctgc cccgcgctgc cggcgttgcg
      601 caggaccacc cgcaggacgg cccactcgcg cttccctccg ccgcgtcctg aggccggcgg
      661 gcatacggtc aggacggtgc actggacccg cagcggagtc cccgtaaggg acttgaggaa
      721 ggctgggtcg aacacgatgg ctcctgacta ctgccgtata gccggagcgc catcctgccg
      781 agcatcgtgc ccgcgccacc agagcggcgg tacggcatac agcggcatgg ccgccaccgg
      841 acacgtgtgg ctacggcacg tcgcgtcgtg cgggccgggc gcgccgcgta cgtccggcca
      901 ggcgcgtcgc gtacgttcgg cgccgcgtcg ggcgaccgcg cgagccgtcc gggccgtcgg
      961 gccacccgtg gctccccggc gagccacggc cgggcagccg tccgcccggc ctcccaccag
     1021 gccgcccgcg gcccaccgct cccggccctc gcggctcggg cgcccatatc gcaaggaccc
     1081 ctaatggtcg agcagcccca tcttggtcgc cgtgatgccc gcctggaagc ggctctccac
     1141 cccgagcctg gccatcagct ccgccatgac ccgccgcaac gtccttaccg acacgccgag
     1201 ttcacgggcg atggcctcgt ccttgcgtcc ggtggccatc agccgcagca cccacagctg
     1261 ctgggagctg aacccggcct cctggcccgt ctccgccgtc tccagcggtg cggccgacgc
     1321 ccagcacatc tcgaagacct tgaccaggag cgcggtcacg gcctcgccgc gcagcaccat
     1381 catggcgttg cttgagttgg tgtgcagcgc gggcaccagg gcctgcaccc ggtcgacgag
     1441 gatgaagcgg aacggcacga tactggcgat ccgcacctgc gcccccgccg cccccagctc
     1501 ctggaggtgg gtcatcgagt ggatgatccg cgtcatcggc cgcaggtgga tgctgcgcat
     1561 caccaccccg cgctccagca cgcgtacgtc acggcgcagc ccctgcgcca gcacctcggg
     1621 agccatcacc gggccggggt gcatggagag gatctccgag gtggcgatgt ccgcggtgtc
     1681 gctgagcacc cgggcgatct tctccggcga gctgaccacc tcaccgctca gctcgctcgt
     1741 gcgggcggtg tgcaggggca ggaacacctc gcgcatggcg tcgatctgct cgcgggtgtg
     1801 ccggatctgg tccgtcagcc gcgcggctgc ctcctcctcg gcggagagga gccgcagcaa
     1861 cgcggcctcg ggcgcggtgg ccttcacggt cggcgcgccg tcggcggagt cctcgcccga
     1921 gtcctcgatg agcaggccga gtacgagcag ctcctttcgt gcccgctcga tctcgtccgc
     1981 ggtcgtgtcg agctgctcgc aggcgctgcg cacggtccag tcgctacggg ccgaggcgta
     2041 gccgaagagc cgcagtgcct gcgggccgat gctcgtcgcg ttcatggcgt ccgctgcctt
     2101 tcgataccgg atcggtgcgg cgcccccgta cgggggaggg gcgtcagctg ccgacaccga
     2161 gcaggtccca cagcccgttg agccgggtgc ggctgccgcc ggccggctcc aggccggagc
     2221 gggtccccgc gaagatcgcg gcggtgacca gatccgcgaa gatggcggag gggaacgcct
     2281 gtgccggctc cccctcctgc ctggcgcgca gcaccagccg ggtgatgtgc gcggcgaccg
     2341 aggagggcag atcctgttcc acgcagccgt ggaaaccgtc cgaggccagc cggacggcgg
     2401 cgccgaagag cggatcgtcg tccatgagtc tggccaactg ttcggccagc aggcgcagtt
     2461 ccttgtaggg gtcgagacac tccgccgcca gctccgcccg cagcgtctcc caggccagcg
     2521 tggcctcgtg ggcgacagcc gcggccagtt cgtccttgga ggagaagtgg ccgtacagcg
     2581 cgcctttcgt catgccgatg tgctcgacca cggttgccag tcgggcgccg gagtagccgc
     2641 gtgcggcgaa ctcctcggct gcggcgtgca ggagcagggc gcgggtcctt accgaccgtt
     2701 cctggctcat cggggttttc tcgcatcttc cccgcctctc ctgtccggtg actgctccat
     2761 gggaatcaaa ataccatccc aaaggtattt ttctgagtgt cttgcctggc ccactccggc
     2821 caggttggcc gaagcgggcc gggacagatc aggtcgcgtg caccaaaccc gtgaccgtac
     2881 cgacgtgttg acgtcgatct gccgtgtcca cgtgccgaag tgccgtgccg gaagccatgt
     2941 gttgggctcc tgttgctgtt gcttgttgct gttgctgctt gcccgttgct gtcgctgcct
     3001 cggtcgtggc tgtcgtcgtc gccgcttccg tcggctgcgg cccggtggtg gcgctcagga
     3061 agcgccagcc cggctccgca cccctctcgg ccagcacggg atcgtcaccg acgaccacgg
     3121 ggtgacccac cagggacagc atcgccaggt cgctgctgtg gtccccgtag gcgcgacact
     3181 cccgcagggg aacgtcgagc cgccgggcca gctccaccac ggcctccgcc ttggccgccg
     3241 cgatcatggg gcgccgcacc tcgccggtga ggaccccgga accgtccgtg agctgctcgg
     3301 tcgccagtac gtgaaggacc ccgaggtgtc cggcgaccgg ccgtacgagg atgtcgccgc
     3361 agccggtgac gagggcgacc tcgtcgccgg aggcgcggtg ctcctccagc gcggccaccg
     3421 tcgcggtcag ataggcgtcg gggccgcgcc ggtagtgcgc gtaccactcc gccgccgcct
     3481 cttcgagccg ccggcgccgc accccagcga agagggtgaa gtaggcccgg ttcagcgtct
     3541 cgcggtccgc gccgctggtg cgcggcgcgg ggacccggcg ccccgagcgg gcgagcgagg
     3601 ccgaccagaa gcgccagaag ctgatcatgc tcttctccgc cagcagcgtc tggtccacgt
     3661 cgaagagcgc gatgcgccgc gtcaccgtgc cccctccgtg gcggcggccc gtgccggggc
     3721 ggcggcctcc tccgcgttgg ccaggggctc cggggcgtgg aggccgccgg tcgcgcgggc
     3781 tcggcgccgg acgaggcctc ggccaccgcg tgctcggggg cggcgccccc gcgaggtccg
     3841 cggccgtgcc gcgccccggt tccgctcctc gcgccgccac cagtgcggag gtgtaactag
     3901 cggcggccgt atcgaagttg agcgtgccgt ggctacgggc cgccgtgatg tcgcgccagg
     3961 cccgctggag tgggtccccc tcgtcgagga cgcgtatccc cgacgacctg aagagttcgt
     4021 cggtggccga gcagcagagt tcgacggcgg ccgtgacgtc ccgctggttc tccgcgacca
     4081 gtacgggggt gaccggagcg tggtcggccc gctgcgcggc ccgctcaagc agcagccgcg
     4141 cggcctggat gtgcgccgaa gcggtggcga gcacacgctg atccgaggcc tggcgggggg
     4201 tgaggccgcc ggccgggtcc agccactcgg ccttcctgtc ccaccaggta cggagcaccc
     4261 cctcacaggc gccgagcacc ggcgcggcga agatcagggc ggcgaccagg gggaagggaa
     4321 ccgtgtgaca gcgcgcccgg tgcgcctgga cccggcccag gtcgcggagg gtgaaggtga
     4381 ggtggccggg gacgaggaca tcggcctcga cggtgctgct gccggtgccg cgcaggccga
     4441 cgggtgccag ttgtcgagga cacgcacctg gtcgcgcggt acggaagatc cggtgctccc
     4501 ggcccccggg cgccgggcca cccgacccgg cggcgggacc cgcccccctc gcgtggccgt
     4561 ccgggtccgg cgcccagagg cgagcaggat ccactccgcc tgccggaccc cgctcgccgc
     4621 ggccaccggc cggtcaaccg cagctgtcgc ccgcgcgttc cgcgctgccc tgcggtggta
     4681 cgaccgaggc ggcgatccgc acgtcagggt gccgccccac agctcgcgct ggcctgcctc
     4741 ggggaggtag gaggcgagcc tgctgtggga ggcgtagagg gcgccgcacc aagcggtgga
     4801 cgcgcaggtc tccgagagca cggagacggc gtccaggagg gcggtgaagg ttcccgcgca
     4861 gccgccccag cgttcgggga cgaagtggcg cccgaatccg tccgtggtca gcgactccgc
     4921 gagccggtcg gtgagggcgc actcctcatc gagccgggcc cggtcctcgc gggcgaagcg
     4981 ccgtgtctgt tcgagatcgg tcagcacgtg gcgacaccgg cctgtgcgat gagcttgccc
     5041 agcggtgagg tgctcgaccc ggtcacccgc gcggtcggca gggtccgcgg cccctgcctg
     5101 gcggtgaggc ggaaggtgtc ggggccttcg ggctccgcgg tgatgacggc ccgcgcgtcg
     5161 agctcgccga aggactcgaa gcccatctgg ataccggtga ccgcccagga gcccggggtg
     5221 gcgtggccgg tgcggaccag tgccgcctgc cggaacgcct cgacgatcac catgccggcc
     5281 acatggtcac ttgcgtggtc gaagagagcg gggtggcggt tgtccatcac gagctgccag
     5341 ccgccatcgg tctcggggct gtcggccagc accaccgcgt cctcacggtc gcgtccgacg
     5401 gcctgcgggg tgaggggtct cggcgggtgg accgctgacg cggcggccct cgggaccggg
     5461 ataccgcggc tccgcagcgt ctggtactgc cggggcgcca ccgcgtccca gcgcatggtg
     5521 gccctgcccg cgggacgccc gccgacccgg atccgcgcct cgcacgacat acggaacctg
     5581 cgcgggttgc ccgcctcgcg gcagacggtg atctcgaaga ccacgtcgga cccgtcgccg
     5641 gtgtcgcgga tgggcgtgtc gagctggaag ctgaaaccgt gcaggacgaa ggcgtggtcc
     5701 atcggcacat cgtggaagcg gtgcgagagg tagatacccg cctgccgtaa ggtctccacg
     5761 acgacgagcg aatccctcgt tccgtcagtg ccccggcggt acaggagatg atcgtgcggc
     5821 cagcggaccc agacggcata gcggtcgtcc gcgagcggtc gtaccgagga caggagcacc
     5881 tcactgtcgg cggccttgtg aaccagagtg cgtggaacat gtgcgtcggc gcggtgtccg
     5941 gcgatcgtag taccggtctc catgtcggtc tcgtacatga cagtcccctc cctgggaatc
     6001 gctgctggcc agcgcatagc tcaagtagcc cttgtttccc ccgacttgag cgtcgcttgt
     6061 gcagcgaaga aaaaatatac ctctgggaag gtatattgca atggccgttc ggtatgaagg
     6121 agacaagggg caaacgcgtc cgaacagcga cgggggagca aggagccgac catgaaggcg
     6181 ggaaccatcg tggagtacaa gcaggacagg gccgttttcc cagttccgga gcgcgcggag
     6241 ttcaccgccg ggcagcaggc cgcccgcagc ccggaactgc accaggaacg agccgtccag
     6301 acgcgcaggc agattctgga gtccgcggcc atactcttcg atcagaacgg gtacgcgggg
     6361 acgtcggtga aggacgtcgc cgaccacgtg ggcatgacca agggcgcggt gtacttccac
     6421 tacccctcca aggaggcgct caccgtcgcg gtggtccagg cgcactacga ccgctggccc
     6481 cggctcctcg aagccgtgca ggccgagggg ctcggcccga tggacaccct ggtcgaactg
     6541 ctcgaccgga cagcgatgac cttcagggac gacgcggtcg tgcagggcgg cgcccgcctc
     6601 atgatggaac agcccctggt ggaaggctcg ttgccgcagc cgtacgtggg ctgggtggac
     6661 ctgatcgaac tgctgctcac ccaggccatg gacgcgggcg aactgcgcga gggcgtgtcc
     6721 cccacggcgg cggcccacac gctggtcgcc gcgttcttcg gcacccagca catctccgcc
     6781 cgactggacc tccgcgtcga tctggtcaaa cgctgggaag aggccaggga cctgctctcg
     6841 tacgcgctgc gcgcctgagg ggtcctgacc ggcgtgccgc gcgcctcggc cgagccccca
     6901 ccgtcctgac ggacgtccgg acccccgctt cacggagcgg gggctcgtga gtgagccggg
     6961 tgtgatcccc gaactccctt acgcgcgagc ggagttctcc gtacgccgac cacggagacc
     7021 gcaaccgccc ccgcgcggct gacgtccgta tcagggaggg ccgcacagcc cgtcctgggg
     7081 gccgtatgaa gattctgttt ctcgccatac ccgcactcat cgcctgcgtc gcgctcggca
     7141 tggcggtgtc ggcgatgcgt gagcggctgc ggctccgcgc cgcctggcgc ggcggactga
     7201 ccgcggaggc gcactgccgg cggacctacg cgacgaccgg caggaggggc ggctcgggca
     7261 cgtccacgac gctccaccac gtgtacgagt tcaccgcgcg ggacggcagg accgtgcggt
     7321 tcgaggagga ggacggcccc gccacgaccc ttgagggcga catcgtggtg gtgagttacg
     7381 cggccgacca cccggagcgg gccacggccc aggagccggg caccgccctg aggcacaccg
     7441 ctcacaccgt cggactgttg ctcttctgcg ctgtgatggt ggcgttctgc gtcttcttcg
     7501 cgacgagcgc cctacggtcg ctctgaccgg cacgccggcc cgtggtcgtt ctcatctccg
     7561 gtccagggcg ggcgtggacg tgcgcgtgga gatggggatg agggtggggt gggggccagt
     7621 gtcctgagcc ccggcgctca ccggcaagtg gtgcaggtcg ccaggcgtgc cgggctgccc
     7681 agggtggtgg tgccgaggcc gatggccctg cgggggcgca ccgtcgtgac cgcggagtgc
     7741 tccgtgcata cggcgatacc gcaccgggtg cagacggccg cggacgcctg ccggcggtcg
     7801 tcgagagcgc actcgtagca aagcattcct gatctcctga gtgagtcggt cgagggtcga
     7861 gtcgtgactg tcacaccaaa ggtccgtttc agcccctggt gtctaaaaag ataggctgta
     7921 gtattgaccc agtcaaatct agactcgatg gctcgacggt gagccgtgaa ggtaggtttt
     7981 cgggatggac gcgttcgcgg aggagctgag agcgaagggg ctgcgcagta cggcccagcg
     8041 gcgtgcggtg ctcaccgcgc tggaaggcgc accgcactcc acggcccccg gcctcgaatc
     8101 ggcgctcggt gtcggacgcc ccggcgcggc ccccgaacgg accggtgatg gctccgggcg
     8161 gaccggcgat gcgcccgcct tctcccggca aggtctctac aacgtgctcg acgacctcac
     8221 gagggcgggc ctggtccgcc gtatcgaacc ggcgggttcc gccgcccgct acgaactgag
     8281 ggtcggcgac aatcaccacc acgtcgtctg taggtcctgc ggcgacgtgg aggacgtgga
     8341 ctgcgtcatc ggcgccgccc cctgcctcga acccttggag gaccggggct tcgtcatcga
     8401 agaagcggag gtcacctggt ggggcgtgtg cggcccctgc cgggctgcgg agcctgcccg
     8461 aggaggctga gccccccccc gggggatagg acatatggcc gcccagagtt caccaagtgt
     8521 gacaccccgt gtccagcggg cctgatggcc cgtcgtggct acggggtgtg atcctcggta
     8581 tgaccctcgg ctgtccgcct gtcgggtgtc tgtcctgtgg acaatcccgg cgtcgtggat
     8641 gtcggacagg gccacggcga gggagcgggc cagctgccgc accttcccgg aaccgagcgg
     8701 tccggacgcg atcatctcgg acagcgcagg cccctcgatt tactcggtga cgaggtaagg
     8761 gcgtccctcg gcggttccgg cgtcgatgac gcccatgccc ccggagccga gacggcggcg
     8821 cagcgtgtag ccgtgtaact cgctcgcatc ggtcggatcc agtggcgccc cgggcatgcg
     8881 tgtctccccc tccgtggtgg tcgatcgaac accgtaccgg cacaagagat caccgactat
     8941 gcggaggtgc ccccgagagc gtgactgagt aagggaaggc gtcgagcatc ggggggcagt
     9001 gatcccatgc gccgacgctg cgcacctgcg ctggacggtg cggcgtgaga ttgcagcgtc
     9061 gaggggaggc ccgcttggcg ggtgtcattg cccggcatgg tgggcgttct gtcgacgtgg
     9121 ctcagatacg aacagcccgg acgaggagcg tccccagatc tccagcatgc ggggcggaga
     9181 ggacgactgc tgacacgtcc atgaagccgt agttgagcag aattccggtc cacatctggg
     9241 gcgtgtaggc ccagcgccgc aggatcgggg ggtcgcctgc aaaggccccc ttgtatgccc
     9301 cctggggtcc gtaacagccc tcgatggctg gtgcgtggga gaacgccagg acgccgcccg
     9361 cgttcaggcg ctcgtgtacg aggggtagca atacggcggg gtcggtgaac cagaggccgc
     9421 cgaagatgga gaacacggcg tcgaaccgat cttcggccct gcggaggaat tcggtcgctt
     9481 ccgtatgaac gaagtgcagg cgttctacgt ctccccattt cacacgggcg cgggagatct
     9541 ggacgtctga caggtccagc cccacggagg tcacaccgcg agtggcgagg tgtgcgatgt
     9601 tacggccgga gccgcagccc agctccagag cgcgttcggg catgccgaag ccctgacggc
     9661 gcaagatctc ctcatcgggg ccatgctccg ggtactgggt ccagttgaac cacgtgcgct
     9721 ctccggcagc attggtctcg cgaggggacg gcttggtctg ggcgtagtcg ccccagaact
     9781 gggttgtggt catgcgtacc ccttacttcg ggctgtcgtg gcacccggcg tggcactggt
     9841 ggcagtccgc tacctccggc cacagcggaa ggtcggcctc gaacccggcc gctgtgatcg
     9901 cgtcgagtgt gcgctgccgg acgttcttcg ctccgccgtg ggtcttctcg tccaggtact
     9961 cagggttgtg gtggatgaag tatcccgcca cccggtcgca gaactcggca tagtccgccg
    10021 tgtgcaggat gaacgtgtgc cacccgacgt cgaccgtctc cgaaggagac agcggctcgt
    10081 cgcactcgac tccggcaacg aggaacttca cggtctccgc catgatgcgg cgggcgtact
    10141 cagggcccat gtgcgggtga tctgccgtga tgcgggtggc gagcctgtcg agcaggtgct
    10201 cacccatgat ctcccggggg tcaaccttct tgatcggtgt ggtgggatcg gtcgtcggca
    10261 tggtgctgcc tcctggcatg ttgggtgcac ttgcggcccg ctgtctgccg ggcttccgtg
    10321 gggtgaggga gcgcttcgcg gaaggatcac gtcaccggca gacagcggga gatcatgcgt
    10381 ccggcttcag gggtgcaccg cagtgcaggc actcccccgt cgtgtcctcc cagatgacca
    10441 ggcagcggca gccagggcac ttggggattt cctcgccttc gatgaagacg ggcgactcct
    10501 cggcgttcgc ctgtggctcc tctgcctgca cggtcatctc acggcctctc gtggggagtt
    10561 gaagcggaca gcatcagtga acggccggac cagagggcgt gaccacgtca gttccggcgc
    10621 agttatgacg cgcatctgct gcatccacta ccgtgaccag ggcagacggc atgcccgaga
    10681 ggacgaggta tggcggacag gcgtgatcgc ttagtgcggc gcatgaaggc gtgcggctac
    10741 agtcaagaac ggttagcgca tgccctgccc gtcgaccgaa ccacggttgg ccgctgggta
    10801 cgcggagagg cagccccgca gggcttcctc tggccgaagc tcgcacgact cctcaaggtg
    10861 accccggcgg agctggagag cctgttatca ccggacgaga ctccagcaac ggcaacgcgg
    10921 tccgaggggg gtgactttga cgacatgatc cgacgcgact tcttgcagct catgacca
//
