LOCUS       NZ_CP045178            25209 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4572354
            Orig. end                         :: 4597563
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..25209
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-i"
                     /region_number="19"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..25209
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..25209
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:15209](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..15209
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            398..3202
                     /locus_tag="GBW32_RS19550"
                     /old_locus_tag="GBW32_19540"
     CDS             398..3202
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150506.1"
                     /locus_tag="GBW32_RS19550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19540"
                     /product="DUF6924 domain-containing protein"
                     /protein_id="WP_193385996.1"
                     /transl_table=11
                     /translation="MFVYRLRLRAPGSAESSPAPPLLVHVDDGMKSAPVPVQLSWTTQD
                     GSQSTVGFSPDMESLYGQRRAPGGDVVEVLGALDDRREYALSAGRTSGYEFSTEVEDAG
                     RWSGAGRLRVLVDDGGRSPVRWVSWCDQAGNTWSVALHAVSPTGNEDVTGLVSDVWASA
                     EHRGAGEVAANLLDARDGKWFAPERTAVLDFRLSQPIEVDQYALTSANDAPDRDPAAWT
                     LSGSTDGKVWRTVDVRDGESFTTRHQRKLYRIAEPGVYDRYRLEITGNNGSPHLQLETV
                     RFLADGGGGFTGYRRRAGYALHPYRGTRVARPQVLLPRPLPERAAEWEGWRPGGSWLPL
                     GGSLSMESLTSTSGRFTVLHGVYDPPLAIRDNLTGEHVWVSDAGSSSLLSLGPDGDLVA
                     WDHHGQRVWSTGTAWRGVRRLEMRDTGELALTDADGGVVWSSGIPEISVSSGEPRTVAR
                     GARMRRGESLYGQTLTSDDGSTVLFHDGRVLRCVVKGHTSHWDRYEDQQNVLVLDAEGF
                     LRRLAPDGSEVERIAGPGAELVVVRGAAVLRDDTGVIVWSSEGGARPAPVREPALPHND
                     TLAAWFGALVGQGHGYCVAVVKQSTPAEVLARIGVASGAAVRGTWRQLQRHRDAVGEGS
                     IAAAVAVGPDVILLSDDAALPVARAVPSAPVAAVRAPSGGNGYGTRFSLHQRGKLVSEF
                     TDQPRRTKGAKVPEVAAALNESTHHLHRFDLLFRVCGVVPNAAGLGGVLLGGVLSGGVL
                     SGGVLSDGVLPGGVPVPHPTPEATPEAEVHLPALVIAEHDQLSPLVIRADFTDDDAWNR
                     VVEGLREPWMDVEPDPYLVSDPAYDGMPAERIIKAVLAAIPETGLPGVVFVADADTMRG
                     PTYPLLAVSTEWDGEPFAEDEDTFRTRFRVRPDAAIEASCNLGLGNMDFEDFAGDGVTE
                     RMAD"
     gene            3350..3760
                     /locus_tag="GBW32_RS19555"
                     /old_locus_tag="GBW32_19545"
     CDS             3350..3760
                     /NRPS_PKS="Domain: Polyketide_cyc2 (10-134). E-value:
                     3.2e-09. Score: 29.3. Matches aSDomain:
                     nrpspksdomains_GBW32_RS19555_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975524.1"
                     /locus_tag="GBW32_RS19555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19545"
                     /product="polyketide cyclase"
                     /protein_id="WP_077966581.1"
                     /transl_table=11
                     /translation="MNSVSRESRHLSVHIDRTADDVYAYASNPVNLPAWARGLGGSIKE
                     IDGQWVAESSPMGRVVVAFVPRNELGVLDHEVTLPSGETVYNPVRAISDGEGSEVVFTL
                     RRQPETTDADFERDAGMVTDDLNRLRELMESA"
     aSDomain        3380..3751
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS19555_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="3.20E-09"
                     /label="GBW32_RS19555_Polyketide_cyc2.1"
                     /locus_tag="GBW32_RS19555"
                     /protein_end="134"
                     /protein_start="10"
                     /score="29.3"
                     /tool="antismash"
                     /translation="LSVHIDRTADDVYAYASNPVNLPAWARGLGGSIKEIDGQWVAESS
                     PMGRVVVAFVPRNELGVLDHEVTLPSGETVYNPVRAISDGEGSEVVFTLRRQPETTDAD
                     FERDAGMVTDDLNRLRELME"
     gene            complement(3906..4475)
                     /locus_tag="GBW32_RS19560"
                     /old_locus_tag="GBW32_19550"
     CDS             complement(3906..4475)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553791.1"
                     /locus_tag="GBW32_RS19560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19550"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_077966582.1"
                     /transl_table=11
                     /translation="MARWQPDAPGRLAAAALDLFEEHGYESTTVIEIAERAGLTKSTFF
                     RYFPDKREVLFDKGTVTGLLVEGIASAPPTAEPLDAMADALDALGRTFFTADRREFSGR
                     RQAVLNANTELREREALKRIDLTASMIEALNRRGVPALTARVAAQLGALVWEIAYDQWI
                     DTDNREGFGPLARDALAEVRAAGALR"
     gene            4559..5479
                     /locus_tag="GBW32_RS19565"
                     /old_locus_tag="GBW32_19555"
     CDS             4559..5479
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (14-189). E-value: 2.5e-34.
                     Score: 110.8. Matches aSDomain:
                     nrpspksdomains_GBW32_RS19565_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984187.1"
                     /locus_tag="GBW32_RS19565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19555"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_077966583.1"
                     /sec_met_domain="ADH_N (E-value: 4.1e-08, bitscore: 31.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 7.2e-10, bitscore:
                     37.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRAVRYRRFGGPEVLELQEIPEPHAAPDEVRVRVAAAGLNPMDW
                     QITTQPDMAARFGITLPAGFGSDFAGVVDEVGAETTGFATGDRVYGAAIGRSVADFVLV
                     RKPEAHLWPTPVGIGDEVAATLPVSGLTAAAALAAIGPRTGDTVLIGGAAGGVGIFAVQ
                     LAKLAGARVLGTASESTFEFLRGLGAEPVAYGPGLADRVRALAPEGITAATDLFGREAA
                     ETALKLGVPPERISVVADGPAPPPGVRKVGALDAGPGALERIADAISSGAIAVPIAATF
                     PVERIREAVTTQAERHVHGKIAIVL"
     aSDomain        4601..5125
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS19565_PKS_ER.1"
                     /evalue="2.50E-34"
                     /label="GBW32_RS19565_PKS_ER.1"
                     /locus_tag="GBW32_RS19565"
                     /protein_end="189"
                     /protein_start="14"
                     /score="110.8"
                     /tool="antismash"
                     /translation="VLELQEIPEPHAAPDEVRVRVAAAGLNPMDWQITTQPDMAARFGI
                     TLPAGFGSDFAGVVDEVGAETTGFATGDRVYGAAIGRSVADFVLVRKPEAHLWPTPVGI
                     GDEVAATLPVSGLTAAAALAAIGPRTGDTVLIGGAAGGVGIFAVQLAKLAGARVLGTAS
                     ESTFEFLRGLGA"
     CDS_motif       4751..4789
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GBW32_RS19565_0001"
                     /evalue="5.80E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="GBW32_RS19565"
                     /protein_end="77"
                     /protein_start="64"
                     /score="9.7"
                     /tool="antismash"
                     /translation="FGSDFAGVVDEVG"
     CDS_motif       4991..5053
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GBW32_RS19565_0002"
                     /evalue="3.00E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="GBW32_RS19565"
                     /protein_end="165"
                     /protein_start="144"
                     /score="22.9"
                     /tool="antismash"
                     /translation="GDTVLIGGAAGGVGIFAVQLA"
     misc_feature    5524..5537
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            5756..6373
                     /locus_tag="GBW32_RS19570"
                     /old_locus_tag="GBW32_19560"
     CDS             5756..6373
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951576.1"
                     /locus_tag="GBW32_RS19570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19560"
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="WP_077966585.1"
                     /transl_table=11
                     /translation="MADMRAFRDAVAGWATGGPDGPARELATSLGARTAVLLEGLSDLA
                     AVEALAARRGRDLAAEGVCVVPMGGAMSVGRYATLLGPPGLGLRLTGLCDENEQGFYES
                     GLTRAGAPSQDIHVCVADLEDELIRALGTAGVEEILGAEGDLRAWQTFQRQPAQHGRPR
                     DQQLRRFLGTKKGRKIRYGRLLVEALTPGRTPPPLDGLLAGV"
     gene            complement(6495..7364)
                     /locus_tag="GBW32_RS19575"
                     /old_locus_tag="GBW32_19565"
     CDS             complement(6495..7364)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046832.1"
                     /locus_tag="GBW32_RS19575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19565"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077966587.1"
                     /transl_table=11
                     /translation="MAAKRGATFRRRELGKELRKLRERQGETLQEAATALGFSPAKLAR
                     VEFGENDLPRTSDLQALIEHLAPDLPRSDLETLLQLHRESLSQEPWMPYKAYMPSKMPT
                     HRGLEQDATVMRGWQPAFVHGLLQTEEYARAIYQLAKPLDETTTEYVERNVRLRLEQKD
                     LITRSDDPLELRVIMDEAAVRRMVGGRDLMRAQYDEIAKLTGLDHVTVQILPHDVVTHR
                     SESNFTILDFAETLAPVVQVDLWNTISVTDGRREVQRAVRRFDAMRESALPPARTVEFL
                     RLVAREWK"
     misc_feature    complement(6987..6989)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7367..7378)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7454..7471
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     gene            7651..8118
                     /locus_tag="GBW32_RS19580"
                     /old_locus_tag="GBW32_19570"
     CDS             7651..8118
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="GBW32_RS19580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19570"
                     /product="ATP-binding protein"
                     /protein_id="WP_107502739.1"
                     /transl_table=11
                     /translation="MTPPTPTPAPPTLAAGGDGVRTVLVEVSIERRPGPGPGGISEADA
                     AWPGRLRRIMRAGLTYWRQPAVIDSAELLLTELVTNALRHGPDHNIDVRVHLRSGRCVI
                     AVTDGSSDRPELRDPGPTEEGGRGLLLVDALACAWGVSSDGTTTWCALPLN"
     gene            8367..9602
                     /gene="fxlM"
                     /locus_tag="GBW32_RS19585"
                     /old_locus_tag="GBW32_19575"
     CDS             8367..9602
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fxlM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490583.1"
                     /locus_tag="GBW32_RS19585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19575"
                     /product="methyltransferase, FxLD system"
                     /protein_id="WP_077966589.1"
                     /sec_met_domain="PCMT (E-value: 1.9e-34, bitscore: 117.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNTTVGPDEATRLRNQVVDELCADGNISSPEIETVMRKVPRHAF
                     IPNTPLDKAYDTYAAVITKTDEHGVQLSSVSAPQIQAMMLEQAKVEPGTRVLEIGSGGL
                     NAAYLAELVGEGGEIVTVDIDPVVTDRARRLLNQQGYGRVHVVTADAAEPIPGLGEVDV
                     VMVTAGAWDISPAWTAQLKQGGRLVVPLRMRGLTRSVAFTQVRGEHGSYLESESARICG
                     FVPMQGSNAHREELLLVNGTPEIGLRFDDGLPADPHRLDNAVTFPRHEMWTNVTVRIQE
                     LIDTLQMAMATSLPGFCTMAVDENSDTGLVDPVNKRFALAAVEGDTFAYLVTRRTEDNK
                     HLEYGVHALGPHAEQFADKIADVLRDWEAKRRGGPSPVIRVYPASTPDDQIPADRVIDK
                     VHSRISLSWPSA"
     gene            9655..9858
                     /locus_tag="GBW32_RS19590"
                     /old_locus_tag="GBW32_19580"
     CDS             9655..9858
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LD_lanti_pre"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380187.1"
                     /locus_tag="GBW32_RS19590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19580"
                     /product="FxLD family lanthipeptide"
                     /protein_id="WP_077966591.1"
                     /sec_met_domain="LD_lanti_pre (E-value: 5.8e-15, bitscore:
                     53.6, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSATLAPPPIAPAPSADPDDDFAPLDVKVVVAEHAYGHLMCSTG
                     DGCGSTCATGASACGSFTEDPA"
     CDS_motif       9655..9771
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MTSATLAPPPIAPAPSADPDDDFAPLDVKVVVAEHAYGH"
                     /locus_tag="GBW32_RS19590_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       9772..9858
                     /prepeptide="core"
                     /locus_tag="GBW32_RS19590_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="-11.40"
                     /molecular_weight="2456.7"
                     /monoisotopic_mass="2455.0"
                     /core_sequence="LMCSTGDGCGSTCATGASACGSFTEDPA"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTSATLAPPPIAPAPSADPDDDFAPLDVKVVVAEHAYGH"
                     /leader_location="[9654:9771](+)"
                     /alternative_weights="2474.7"
                     /alternative_weights="2492.7"
                     /alternative_weights="2510.7"
                     /alternative_weights="2528.7"
     gene            10001..13012
                     /locus_tag="GBW32_RS19595"
                     /old_locus_tag="GBW32_19585"
     CDS             10001..13012
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380186.1"
                     /locus_tag="GBW32_RS19595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19585"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_306292938.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 5.7e-199,
                     bitscore: 661.0, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.1e-17,
                     bitscore: 62.2, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.2e-14, bitscore:
                     51.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRATTSPGPADIPRTLDLDNVAVTRGWLNRIWQREDFRSALEMA
                     TPVLSDAIDSVVQGRQTESRHVRRTAVSTVSYLLRWEHRPTPLGLFAGVAPVTVGPGTT
                     ARWREKHRAMIRPDAEWVTDMVLRLQRTPALLKRLPLVANNAARARGDRLVAPGPPSDG
                     HAVLLAPVEVSVRNARPVAVAMEAARAPIRYADLHDRLRDSFPQATPEKIDALLTGLVE
                     QQFLITSLWAPMTTMDAFQHLCRELDRHQADSVPEVRDLVHALYALRDDVPTHQPTLSD
                     TSVSALVTRMRAITPVTPTPLLIDTVLGCETQLPPAVIAEAQAAVSALYRTTAQPYGYQ
                     HWRDYHRRFRARYGVGALVPVMDLVADSGLGLPAGYVGSERGAAPKVRTDRDELLLALM
                     QQVFLDGRDELRLTDDMVNALEKAAGTDERLFVPRVEAAFEVLSPSTRALSSGTFEVLL
                     TAVPRPGSSMAGRFAHLLPSEHRDALAATYRGAPDLLTAQLVFPPRRHRNENVTRTPRL
                     LPHLIELSGHQETDETTIRLADIAVTADPRSFHLVQLATGRRIDVRVLHALEGGTQTPP
                     LARFLAEVANARCAAYKPFDFGAGARLPFLPRVRYRRTILAQARWLLMADDLPGRTAST
                     RQWEDAVAAWRDRLRAPTQVAIVEYDQRLPLDLSHPVHRQVLRARLDNTRRLELREVPD
                     ADAHGWIGRAHEIWLSLSRLQPDTAEFPRRRTATAPAPGRQLPGGGYVLLARLHAHPRR
                     YDEILSRHLPRLLAAFGEMTPVWWFSRYREMARPDADQHLDLTLHLPPDAYGTAAQHVR
                     RWADSLHAAGLAAGLVLAPYQPQTGRFGGEAAMDAAHQVFAADSAAALAQIQLTERTDT
                     LAPQALAAASALHLVVPLAPDIEEWLVRNLPQGTGRLDRALRTQVLELAAPNGAAVLAA
                     LPSGPEVTQAWQARAVAVDTYRNTLAGQRDPLDVVRSLLHQHHVRALGVDPHAEETTIR
                     LVRTAALQHRMAVR"
     misc_feature    10403..10405
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        10424..10699
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_GBW32_RS19595_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="8.60E-20"
                     /identifier="RREFam001.1"
                     /label="GBW32_RS19595"
                     /locus_tag="GBW32_RS19595"
                     /protein_end="233"
                     /protein_start="141"
                     /score="62.2"
                     /tool="antismash"
                     /translation="VANNAARARGDRLVAPGPPSDGHAVLLAPVEVSVRNARPVAVAME
                     AARAPIRYADLHDRLRDSFPQATPEKIDALLTGLVEQQFLITSLWAP"
     gene            13009..14238
                     /locus_tag="GBW32_RS19600"
                     /old_locus_tag="GBW32_19590"
     CDS             13009..14238
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490581.1"
                     /locus_tag="GBW32_RS19600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19590"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_077966595.1"
                     /sec_met_domain="LANC_like (E-value: 9e-34, bitscore:
                     115.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQAVTAAVLLEQYTAQLAQPAPPPPDEPWAVQSLADGAAGISL
                     LHIEMASHHGGSWRPAHRWITAAAAGHISAADTAGLFLGAPALGFALTTVPPSFQHLYE
                     PARRTLHRHIIDLTHRRVDAALTRIRKGDLPTFAEYDLFHGLTGIGAYLLRTDPEGRAL
                     ERVLKYLVALTRPLAPDSRNLPGWWVRHDPARGESPHFPGGHGNFGAAHGITGPLLLLA
                     QAMRRDITVSGHREAIWSVCEHLDAWCQLRPSGPWWPEHISRRDLDLGRPHHDAPTRPS
                     WCYGVTGIARAGQLAGIALRAPDVQTYYEDALYRALTDPEQLARITDSGLCHGWAGIYQ
                     TATRAAADALDPRLQILTKGLGDNLAANARPGLPEPGLITGDAGSALALTTYASQQTPM
                     TGWDACLLIN"
     gene            14220..15209
                     /locus_tag="GBW32_RS19605"
                     /old_locus_tag="GBW32_19595"
     CDS             14220..15209
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490580.1"
                     /locus_tag="GBW32_RS19605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19595"
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="WP_077966597.1"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.4e-35, bitscore:
                     122.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLDQLILTEPSPIQRAVTAALTGLPIADTAAQYGMEPTTLSDAV
                     AVYDQAGREALSRHEANTDWRQAYLHFTDWADTDTTFTAYVLPVLREAEAAGIIGGWWY
                     TRKHPCWRLRLRLQPEAGTELPLNERLDRLVSDGHLRRWWPGIYEPETAAFGGAAGMTA
                     AHALFVTDSREAPQLGQRQDLAVGPRELSVLLCTIMMRAAGLEWYEQGDVWHRVITQEH
                     RSAVSDVPADRLDARAQEIRPLLFADSEVLLRPGGLLEPVTEWVAVFRSTGQELRRAVE
                     AGTLQRGLRQVLGYHVIFHWNRLGLSLRTQSILAWAAREAILHHNSPT"
     gene            15456..16727
                     /locus_tag="GBW32_RS19610"
                     /old_locus_tag="GBW32_19600"
     CDS             15456..16727
                     /EC_number="2.7.2.4"
                     /GO_function="GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063111.1"
                     /locus_tag="GBW32_RS19610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19600"
                     /product="aspartate kinase"
                     /protein_id="WP_077966599.1"
                     /transl_table=11
                     /translation="MGLVVQKYGGSSVADAEGIKRVAKRIVEAKKNGHQVVVVVSAMGD
                     QTDELIDLAEQVSPMPDGREFDMLLTAGERISMALLAMAIKNLGHEAQSFTGSQAGVIT
                     DSSHGKARIIDVTPGRIRTALDEGNIAIVAGFQGVSQQKKDITTLGRGGSDTTAVALAA
                     ALDAEVCEIYTDVDGVFTADPRVVPKARKIESIAFEDMLELASSGSKVLLHRCVEYARR
                     YNIPIHVRSSFSGLKGTWVSSEVRGDQTMEQAIISGVAHDTSEAKVTVVGVPDKPGEAA
                     TIFRTIADSEINIDMVVQNVSAASTGLTDISFTLPKSEGRKAIDALEKTKPKVGFESLR
                     YDDQIAKISLVGAGMKTNPGVTAGFFEALSDAGVNIELISTSEIRISVVTRADDVNEAV
                     RAVHTAFGLDSDTDEAVVYGGSGR"
     gene            16761..17816
                     /locus_tag="GBW32_RS19615"
                     /old_locus_tag="GBW32_19605"
     CDS             16761..17816
                     /EC_number="1.2.1.11"
                     /GO_function="GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA]"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032009.1"
                     /locus_tag="GBW32_RS19615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19605"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_077966601.1"
                     /transl_table=11
                     /translation="MTVRPTLAVVGATGAVGTVMLQILSQHADIWGEIRLVASPRSAGR
                     KLTVRGEEVEVVAVSEEAFDGVDIAMFDVPDEVAARWAPVAAAKGAIVVDNSGAFRMDP
                     DVPLVVPEVNPHAARRRPRSIVASPNCATLSMIVAIGALHAEFGLRELIVSSYQAVSGA
                     GRAGIETLRSQLAMVAGTELGTSPGDVRRSVGDETGPFPAPLALNVVPWVGSLREDGWS
                     SEEMKVRDESRKILGLPGLKVTATCVRVPVVTTHSMTVHARFEDEVTVDKAREILATAP
                     GVVLFDDPAEGEFPTPADVVGTDPTWVGRVRRSLDDTAALELFVCGDNLRKGSALNTAQ
                     IAELIASEFTA"
     gene            complement(17831..18052)
                     /locus_tag="GBW32_RS19620"
                     /old_locus_tag="GBW32_19610"
     CDS             complement(17831..18052)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS19620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_19610"
                     /product="hypothetical protein"
                     /protein_id="WP_077966603.1"
                     /transl_table=11
                     /translation="MPETAGTRGNARDGICYVAYATHATHAVKAATAGEAVKATTAAAT
                     GTAVKATKSTTVGESPKAVEAVEAVEAA"
     gene            18371..19048
                     /locus_tag="GBW32_RS19625"
                     /old_locus_tag="GBW32_19615"
     CDS             18371..19048
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010350867.1"
                     /locus_tag="GBW32_RS19625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19615"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_077966605.1"
                     /transl_table=11
                     /translation="MAEVLEFTAVQVPGAVLHPLRRPRMPGAPGSMPVIAPMPVTRPTA
                     RIPGQREGADDTTPDSAGTTVDHLTETYRTHYRSLLGLAALLLDDTASCEDVVQEAFIR
                     VHSARKRVRDPEKTLAYLRQTVVNLSRSALRRRILGLKLLSKPMPDMASAEEGAYDQLE
                     RDALIKAMRGLQRRQREVLVLRYFADMTEAQVAKTLGISLGSVKAYGSRGIAALRVVME
                     AQA"
     gene            19294..20427
                     /locus_tag="GBW32_RS19630"
                     /old_locus_tag="GBW32_19620"
     CDS             19294..20427
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS19630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_19620"
                     /product="hypothetical protein"
                     /protein_id="WP_077966607.1"
                     /transl_table=11
                     /translation="MSGESAIGAHTSEGESGVSRESGEGPEGPGPTPLGPSGVDEDSLR
                     DLLHHAVQGIEPSDQALDRLQRAVPARRTRKRQAVVGVAAAAILIGTAVPALVHVTAEP
                     SGGHDHPAIAGDTERTEIGAGLEDDRHGRAPGGHSPSDKSPGHTKGGREDSEETKGAKD
                     KDTTEPADGAEPTGSTAASGPPECDPGQLGAPKATTSQPDKDGKVYGTFKIANVSGKKC
                     VIEGEGSVAAAALGAADPAKIAVVEHMPGDAATKLPPPSRAERALMLEPGTSYEVRFAW
                     VPSADCHTDDHPGEGTGSVSPDPSPSGDTEIDRSASPDVKSGTNDVSPQLLREADGDED
                     GSVSISHTAERGGPTADTTIPDACAGTIYRTGVLQPQ"
     gene            complement(20583..21581)
                     /locus_tag="GBW32_RS19635"
                     /old_locus_tag="GBW32_19625"
     CDS             complement(20583..21581)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762634.1"
                     /locus_tag="GBW32_RS19635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19625"
                     /product="SURF1 family protein"
                     /protein_id="WP_077966609.1"
                     /transl_table=11
                     /translation="MYRFLLTPRWWGINVFVVLAIPFCVFMGSWQLSRFEDRMDAQHSA
                     EQAPAKAAHQRAAPLRNLLPVDQKTSGRTAETSGRYVRQLLVPGRVVEDKQGFYVLGLL
                     RTDGGGGDKGKVLPVVRGWVPGHASAAKAPALPTGEVSVRGALQSSENTGTDGVSTSGG
                     LPQGQLGMISAASLVNLTSYDVYDAWITLDKGDSGMRAVPAAAPPDTGLDLKAFQNLGY
                     TGEWFVFAGFVVFMWFRLLRREAEAARDEALGIPAPEPEPFMFVTGSGGSGGSVPAPAA
                     AAAPASAPASAAGGDAAPDSASAGGTAADAAPTDEATVGGPVARSGSDTRG"
     gene            complement(21780..23611)
                     /locus_tag="GBW32_RS19640"
                     /old_locus_tag="GBW32_19630"
                     /pseudo=""
     CDS             complement(21780..23611)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762635.1"
                     /locus_tag="GBW32_RS19640"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_19630"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSDAEAMPDWEKRFRAPRVSLPEWAEDAPDRSLFVSNATGTYELY
                     AWDRATGEQRQVTDRANGTTDGVLSPDGRWIWWFDDTDGDEFGVWMRQPFEGGDDVPAV
                     EGLAPAYPAGLAIGRDGATVVVGSSTDDEGSTVHVVHGAGGAPVEIYRHPESAGVGDLS
                     HDGSLVVIEHTEHDNAMHSALRRCGCCGPRPKARRAPRRRSRSSTTPRAGRRSWACPSS
                     ASRRSRVTRVSSSGTSAGAAGSRWCGTSPPVRSASWSSGCPAT"
ORIGIN
        1 cgcgttcatc aacgcgctgg ccgaactccc cgaccccgac caccccgcgt accgcatcgc
       61 cgcggaccag aagcgctggc tgctggagct gttcgaggaa ctcgccaccg cggcgggccg
      121 cccggacccc gccaccctcg ccgcccggct cctcgtactg cacgagggcg ctctcgccac
      181 gcagccgatc gcgctcccgc cgcacgacat gctttcggtg agtaccgacc tggcacgcgc
      241 cctggttcgg gccgacggcg cgtgaactcg cacgtgagcg ggtggtgtca gtgtcgacgc
      301 ctacgatcac ctgcgtcctg tggtacttga gcgggcgccc ctccggcgcc gtggtgagcg
      361 aggcggatgt atgccagcgg tttcgagtga gggttccgtg ttcgtgtatc gcctgcggct
      421 gcgcgcaccc ggctccgcgg agtcgtcgcc cgcgccccct ctgctcgtgc acgtggacga
      481 cggcatgaag tcggcgccgg taccggtaca gctgtcctgg accacgcagg acggctccca
      541 gtccacggtg ggcttctcac ccgacatgga gagcctgtac ggccagcgtc gcgcacccgg
      601 cggcgatgtg gtggaggtgc tcggtgcgct cgacgatcgt cgcgagtacg cgctctcggc
      661 cgggcgtacc agcggatacg agttctccac cgaggtcgag gacgccgggc gctggagtgg
      721 cgccggacgg ctgcgggtgc tggtggacga cggcgggcgg agccccgtgc ggtgggtgtc
      781 ctggtgcgac caggcgggca acacgtggtc ggtcgcgctg cacgccgtga gcccgaccgg
      841 caacgaggac gtcaccggcc tggtgagcga cgtgtgggcc agtgccgagc accgcggggc
      901 gggcgaggtg gccgcgaatc ttctggacgc gagggacggc aagtggttcg cgccggagcg
      961 caccgcggtc ctggacttcc ggctgagcca gccgatcgag gtggaccagt acgcgctgac
     1021 ctccgccaac gacgcaccgg atcgcgaccc ggcggcatgg actctgagcg gctcgacgga
     1081 cggcaaggtc tggcgcaccg tcgatgtccg cgacggcgag tcgttcacca cgaggcacca
     1141 gcgcaagctg taccggatcg ccgaaccggg ggtctacgac cgctatcgcc ttgagatcac
     1201 cggcaacaac ggctccccgc acctgcaact cgaaaccgtc cggttcctgg ccgacggagg
     1261 cggcggtttc accggctacc gccggcgcgc cggctacgcg ctccacccct accgaggcac
     1321 acgtgtggca cggccgcagg tgctgctgcc gcgaccactg cctgagcgcg cggccgagtg
     1381 ggaggggtgg cggcccggcg gatcgtggct gccgttgggc ggcagcctct cgatggaatc
     1441 cctgacgtcg acgtcgggcc ggttcacggt tctgcacggg gtgtacgatc cgccgctggc
     1501 gatccgcgac aacctcacgg gcgagcacgt ctgggtcagc gacgccgggt cgtcgagcct
     1561 gctctccctg ggccctgatg gtgacctcgt cgcctgggac catcacggcc agcgggtgtg
     1621 gagcacgggt accgcgtggc ggggcgtgcg gcggctggag atgcgcgaca ccggcgaact
     1681 cgctctgacc gacgccgacg gtggcgtcgt gtggagctct ggcatccccg agatatccgt
     1741 atcgtcgggc gagccgcgca cggtggccag gggagcgagg atgcgccgcg gcgagagcct
     1801 gtacggccag acgctcacca gcgatgacgg gtcgaccgtg ctcttccacg acggtcgcgt
     1861 cctgcgctgc gtcgtcaagg gccacacctc acactgggac aggtacgagg accaacagaa
     1921 cgtcctggtg ctcgatgccg agggttttct gcggaggctg gcgccggacg gctccgaggt
     1981 ggaacggatc gccgggcccg gcgccgaact ggtcgtcgta cgaggcgccg cggtactcag
     2041 ggacgacacg ggcgtcatcg tgtggtcgtc cgaaggcggc gcccgcccgg cccccgtccg
     2101 tgagcctgcc ctaccccaca acgacacgct ggcggcctgg ttcggggcac tggtcggtca
     2161 gggccacggg tactgcgtcg cggtggtgaa gcagagcacg cccgctgagg tcctcgcacg
     2221 catcggtgtc gcgtcgggcg cggcggtgcg cggtacgtgg cgccaactcc agcggcaccg
     2281 ggacgcggtg ggcgagggga gcatcgccgc ggcggtcgcc gtcggccctg acgtcatact
     2341 tctgagcgac gacgccgcac tgccggtggc gcgggcggtg ccctcggccc cggtggccgc
     2401 cgtgcgggcg ccgtccggtg ggaacggcta cgggacacgg ttcagcctcc atcaacgcgg
     2461 caagctcgtg agcgagttca cggatcagcc tcgccgtacg aagggggcca aggtgcccga
     2521 ggtggccgcc gcgctgaacg agagcacgca tcacctgcac cgcttcgacc tgctgttccg
     2581 tgtctgcggg gtggtgccga acgccgcagg gctcggcggt gtgctgctcg gcggcgtgct
     2641 gtccggcggt gtgctgtccg gcggtgtgct gtccgacggt gtgctgcccg gcggtgtgcc
     2701 ggtcccgcac ccgactccgg aggcgacgcc cgaggccgaa gtccacctgc ccgcgttggt
     2761 gatcgcggag cacgaccagc tcagccccct ggtgatccgc gccgacttca ccgacgacga
     2821 cgcctggaac cgcgttgtcg agggactccg ggaaccgtgg atggacgtgg agccggaccc
     2881 gtatctcgtc tccgatcccg catacgacgg catgcccgcg gagcggatca tcaaggccgt
     2941 cctcgcggcg atacccgaga ccggactgcc cggcgtggtc ttcgtcgccg acgccgacac
     3001 catgcgcggc ccgacgtatc cgttgctcgc cgtgtccacc gagtgggacg gtgaaccgtt
     3061 cgcggaggac gaggacacct tccgcacgcg gttccgcgtc cggcccgacg cggccatcga
     3121 ggcgagctgc aacctcggcc tcggcaacat ggatttcgag gacttcgccg gcgacggggt
     3181 gaccgaacgc atggcggact gacggcccct gcacctgtcc ctgccccttc ccctgccctt
     3241 ggccctgtcc ctgtccctgt cctttccggt gggtcgagac cagtgcgtgc gtggagcgtg
     3301 tccgattcgt tcaagagggc ggcggcatcg gcccactact ctcacggcca tgaacagcgt
     3361 cagcagagaa tcacgccacc tcagcgtcca tatcgaccgc acggccgacg acgtctacgc
     3421 ctacgcgtcg aacccggtga acctgcccgc gtgggcccgt ggcctgggag gttccatcaa
     3481 ggagatcgac ggccaatggg tcgcggaatc ctctcccatg ggacgggtcg tggtggcctt
     3541 cgtgccgcgc aacgaactgg gcgtactcga ccacgaggtc acgctgccct ccggggagac
     3601 cgtctacaac ccggtccgtg cgatcagcga cggcgaggga agcgaggtcg tgttcaccct
     3661 ccgcaggcag ccggagacga ccgacgccga tttcgagcgc gacgcgggca tggtcaccga
     3721 tgacctgaac cggctccgag agctgatgga gtccgcgtag agaggacgtg gatccgcctt
     3781 ggagagacgc agagagacgc agagagacat agagagacgc agagagacgt aaagccgcta
     3841 gtgccgcgtc aggcatcgtc gacgttcgcc ctgatcctga tcctgatcct gatcctgata
     3901 cggcactagc gcagggctcc ggctgcgcgt acttcggcga gcgcgtcccg ggccagcggg
     3961 ccgaagccct cgcggttgtc ggtgtcgatc cactgatcgt aggcgatctc ccagacgagc
     4021 gcgcccagtt gggcggccac gcgcgcggtc agggccggca ctccgcggcg gttgagggcc
     4081 tcgatcatcg aggcggtgag gtcgatcctt ttcagggcct cgcgttcacg cagttccgta
     4141 ttggcgttca gcacggcctg gcgccggccg ctgaactcgc ggcggtcggc ggtgaagaac
     4201 gtccggccga gcgcgtcgag ggcgtccgcc atcgcgtcga gcggctcggc cgtcggcggt
     4261 gccgaggcga tcccttcgac gagcaggccg gtcaccgtgc ccttgtcgaa aagcacctca
     4321 cgcttgtccg ggaagtaccg gaagaacgtg ctcttggtga gccccgcgcg ctccgcgatc
     4381 tcgatcaccg tcgtgctctc gtagccgtgc tcctcgaaga ggtcgagggc ggcggcggcg
     4441 agtcgtccgg gtgcgtcggg ttgccagcga gccatgggag cagtgtacgg gacttcgtcc
     4501 catcactggt gtacggtcca tgagaccaag tcccatcact ggccaggaga ggtcaccgat
     4561 gagtagagct gtccggtacc ggcgctttgg cggtcccgaa gtactcgaac tgcaggagat
     4621 acccgagccg cacgccgcgc cggacgaggt tcgcgtccgg gtcgcggcgg ccggtctgaa
     4681 cccgatggat tggcagatca ccacccagcc cgatatggcg gcgcggttcg gcatcacctt
     4741 gccggcgggg ttcggcagcg acttcgccgg agtggtggac gaggtgggcg cagaaaccac
     4801 gggattcgcg accggtgacc gggtgtacgg ggctgcgatt ggccgatccg tcgccgattt
     4861 cgtgctggtc aggaaacccg aggcacacct gtggcccacc ccggtgggga tcggtgacga
     4921 ggtggcggcc acgcttccgg tctccggtct gacggctgcc gccgcgctcg ccgcgatcgg
     4981 accgcgcacc ggagacaccg tcctgatcgg aggagcggcg ggtggcgtgg gcatcttcgc
     5041 cgtgcagttg gcgaagctcg cgggtgcccg ggtactcggc accgcctccg agagcacgtt
     5101 cgaattcctg cgtgggctgg gcgccgaacc cgtggcgtac ggccctggcc tggcggaccg
     5161 ggtgcgggcc ttggcgcccg aggggatcac cgccgcaacc gacctgttcg gaagggaggc
     5221 cgccgagacc gctctcaagc tcggcgtgcc acccgagcgg atctccgtcg tagccgacgg
     5281 ccccgccccg ccgcccggtg tgcgcaaggt gggcgccctc gatgcggggc cgggcgccct
     5341 ggaacggatc gccgacgcga tcagctccgg cgcgatcgcg gtgccgatcg cggcgacctt
     5401 cccggtcgag cggattcgcg aagccgtgac gacgcaggcc gagaggcacg ttcacggaaa
     5461 aatcgcgatc gtactgtgaa tcggcgcacg tgtgcggcca cttgctcggc ctgacgcgac
     5521 cgcgccaccc tgagcgacgg ctcggacggg acatcacatc agggcgaagg ttctctgctg
     5581 ccgcgccaga tccgacgtgg atccggcagg acgcctcatg gtcggcgcgc aggattcgcc
     5641 gggccccctc accctgtgcg gtgtcggctg tacacgtgcg aaatcccgtg cggtgtcggc
     5701 tgtacacgtg cgaaatcccg tgccgcttcg gccgtacacg tgcagacttc gccccatggc
     5761 ggacatgagg gcgttccggg acgcggtggc ggggtgggcg acgggcggcc ccgatgggcc
     5821 cgcgcgggag ctggcgacca gcctgggggc gcggacggcg gtgctcctgg aagggctgag
     5881 cgatctcgcg gccgtcgagg cgctggccgc ccggcgtggc cgcgacctgg cggccgaggg
     5941 ggtgtgtgtc gtgccgatgg gcggagcgat gagcgtcggc cgctacgcca ctctcctcgg
     6001 gccgcccggc ctcggtctgc gcctcaccgg actgtgcgac gagaacgaac agggcttcta
     6061 cgaaagcggg ttgacgcgcg cgggggcacc gtcgcaggac atccacgtat gcgtggccga
     6121 cctggaggac gaactcatcc gcgcgctggg tacggcggga gtcgaggaga tccttggcgc
     6181 ggagggcgat ctgcgagcct ggcagacctt ccaacgccag cccgcgcagc acggccgccc
     6241 ccgtgaccag cagctacgcc gtttcctcgg cacgaagaag ggccgcaaga tccgctacgg
     6301 ccgcctcctg gtcgaggcgc tcacaccggg acggacaccg ccgccgctcg acgggcttct
     6361 tgcgggggtg tgagcgacgg agcgccggta gcgcggcctt gctcgactca tgaccgcctg
     6421 agtcgtcgcc gtacgagggc tcgtgtcagt cggacttcag gtccgcatcg agggccacgt
     6481 cccggaactg ctggctattt ccactctcgg gccaccagcc gcaggaactc gactgtccgc
     6541 gctgggggca gggcgctttc gcgcatggcg tcgaagcggc ggaccgcccg ctgcacctct
     6601 cgccgcccgt cggtaacact gatcgtgttc cacaagtcca cctgtacgac aggggcgagg
     6661 gtttccgcga agtcgaggat cgtgaagttg ctctccgagc ggtgcgtcac gacatcgtgc
     6721 ggcaggattt ggacggtcac gtggtccagc ccggtcagtt tcgcgatctc gtcgtattga
     6781 gcgcgcatga gatcacgacc gcccaccatg cgccgaacgg ctgcctcatc catgatcact
     6841 cgcaattcca gcggatcatc cgaacgagtg atcaggtcct tttgctccag ccgcaaccgt
     6901 acgttccgct cgacgtactc ggtggttgtc tcgtccaacg gtttggcgag ctggtaaatg
     6961 gctcgggcgt actcctctgt ctggagtaag ccgtgcacga aggcgggctg ccagccacgc
     7021 atgactgtgg cgtcctgttc gagacctcgg tgggtcggca tcttcgacgg catgtacgcc
     7081 ttgtacggca tccacggctc ttggctcaga gactcccggt ggagctggag cagagtctcc
     7141 agatcgcttc tggggagatc aggggccagg tgctcgatga gcgcctgtag gtcactggtg
     7201 cgtggaagat cgttctcacc gaactcgacc cgcgcgagct tcgcagggga gaagcccaga
     7261 gccgtggcgg cctcttgaag tgtttccccc tgcctctccc ggagcttgcg aagctccttg
     7321 ccgagttctc tgcgccggaa cgtcgcccca cgtttagctg ccacgcgtaa acctttcccc
     7381 agagctatag gtgttcaaca cgccttctgc cctgcgctgt tgctggtcct gtccctgagt
     7441 gtgacacccc ctgtaccccc ttgagtggct tctgtgtacg tcctggcatt taccaattcc
     7501 gttgtcgcgg cggtttctct tgcacggcaa atcgcacatg cagcacggtg aacgagtagc
     7561 cgtcaggacc ggccggtgag gaaccacgcg aactgtgcgc gggggcgcat cccgtggccg
     7621 acagcgctga cgttcatcgg aggtccccgc atgacacccc ccacgcccac acccgcacct
     7681 cccacgctcg ccgcaggggg tgacggggta cggaccgtcc tcgtcgaggt ctctatcgag
     7741 cggcgtcctg gtcctggccc cggaggcatc tccgaggccg acgcggcctg gcccggacgg
     7801 ctgcgccgca tcatgcgggc cggcctcacc tactggcgcc agcccgccgt gatcgactcc
     7861 gctgagctgc tgctgacgga actcgtgacc aacgcgctcc gccatggacc cgatcacaac
     7921 atcgatgtcc gcgttcacct gcggtccggc cggtgcgtga tcgcggtgac cgacggttcc
     7981 agcgaccgtc ccgagctgcg tgaccccggt cccacagagg agggcgggcg gggactccta
     8041 cttgtcgacg ccttggcctg cgcgtggggt gtcagctccg acggcacaac gacctggtgc
     8101 gcccttcctc tgaactgaag gacctctgag atgaagccca ccgcggcaac cgtgccgttc
     8161 tccgaatggt ccgccccgcg ccgggtcccg cagcaacaac cgatggagac ccgtgaccag
     8221 cgccgagtcc tcgcgcgctg tccatcacga cccgccaacc ttcggcagcc cgacaagagc
     8281 ggagagtgtc cgccgatagc ctcccggccc accggcacgt gacgccggcc tactaccccc
     8341 cgaacacgaa cgatcgaagg aagtccatga cgaacacaac cgtggggccc gacgaggcga
     8401 cccgcctgcg gaaccaggtg gtggacgaac tgtgcgcgga cggcaacatc tcctccccgg
     8461 agatcgaaac ggtgatgcgc aaggtgcccc ggcacgcgtt catccccaac accccgctgg
     8521 acaaggcgta cgacacatac gccgccgtga tcaccaagac ggatgagcac ggcgtgcagc
     8581 tcagctccgt ctccgcgccg cagatccagg ccatgatgct ggagcaggcc aaggtcgagc
     8641 ccggaacgcg ggtgctggag atcggatcgg gcgggctgaa cgcggcctac ctcgccgaac
     8701 tcgtcggtga aggcggtgaa atcgtcaccg tggacatcga ccccgtcgtg accgatcgcg
     8761 cgcgccgact cctgaaccag cagggctacg gccgggtgca cgtcgtcacc gccgacgccg
     8821 ccgagcccat cccgggcctc ggtgaggtcg atgtcgtgat ggtgaccgct ggagcctggg
     8881 acatctcccc ggcctggacc gcgcagctca agcagggcgg ccggctcgtc gtcccgctga
     8941 ggatgcgcgg cttgacccgg tcggtcgcct tcactcaggt caggggcgaa cacggctcct
     9001 acctggagag cgagtcggcg cggatctgcg gctttgtccc gatgcagggc tccaacgccc
     9061 accgggaaga actcctcctc gtcaacggca ccccggagat cggactcagg ttcgatgacg
     9121 gccttccggc cgacccgcat cggctcgaca acgccgtcac gtttccgcgc cacgagatgt
     9181 ggaccaatgt caccgtccgc atccaggaac tcatcgacac cctccagatg gcgatggcca
     9241 ccagcctgcc gggcttctgc acgatggccg tcgatgagaa ctcggacacg ggcttggtcg
     9301 accccgtgaa caagcggttc gccctcgcgg ccgtcgaggg cgacaccttc gcctatctcg
     9361 tcacccgccg caccgaggac aacaagcacc tggagtacgg cgtgcatgcc ctcggcccgc
     9421 acgcggagca gttcgccgac aagatcgccg acgtgctccg cgactgggag gccaagcggc
     9481 gcggcggccc cagcccggtc atccgggtct atccggccag tacccccgat gaccagatcc
     9541 cggcagaccg ggtcatcgac aaggtgcaca gccggatctc gctctcctgg ccctcggcgt
     9601 agcccccggg agccaggtag tcccgcacca tccgaacaag aggaaggacc catcatgacg
     9661 agcgccactc tcgccccgcc tccgatcgcg cccgcgccgt cggcggaccc ggacgacgac
     9721 ttcgcacccc tggacgtgaa ggttgtcgtc gccgagcacg cgtacggcca cctcatgtgc
     9781 tccacgggcg acggctgcgg ctccacctgc gcgaccggtg cctccgcctg cggttccttc
     9841 accgaggacc cggcctgatc caggccgact ctcacccggt gggccgggcg aagcgcttcg
     9901 cccggcccac cgggccctct gtccctcccc gcgccgcctc atggaaggac ctcatgtggc
     9961 ctctcaaaca gtcgccggat accagtggca aggagttgcg atgctgcgcg ccaccaccag
    10021 ccccgggccg gcagacatcc cccgcaccct cgacctggac aacgtggccg tcacccgcgg
    10081 atggctgaac cgcatctggc agcgcgagga cttccgcagc gcactggaga tggccacccc
    10141 cgtcctgtcc gacgccatcg actccgtcgt ccaaggccga cagaccgagt cccgtcacgt
    10201 ccgccgcacc gcggtgtcga cggtgtccta cctgttgcgg tgggagcacc ggccgacccc
    10261 gctcggcctg ttcgccggcg tcgccccggt gacggtcggg cccgggacga ccgcgcggtg
    10321 gcgcgagaag caccgcgcga tgatccggcc ggacgccgaa tgggtcacgg acatggttct
    10381 ccgcctccag cgcacccccg cgttactgaa gcggctaccg ctcgtcgcca acaacgccgc
    10441 ccgcgcccgc ggtgaccggc tcgtggcgcc gggcccgccc tccgacggtc acgccgtcct
    10501 cctggccccg gtcgaggtat ccgtccgtaa cgcccggccg gtagccgtcg cgatggaagc
    10561 ggcccgcgcg cccatccgct acgccgacct gcacgaccgc ctgcgcgaca gcttcccgca
    10621 ggccacaccg gagaagatcg acgcccttct caccggcctg gtcgagcagc agttcctcat
    10681 caccagtctc tgggcgccca tgaccaccat ggatgccttc cagcacctgt gccgtgaact
    10741 ggaccggcac caggcggaca gcgtccccga ggtacgggac ctcgtccacg cgctgtacgc
    10801 gctgcgcgac gacgtgccca cccaccagcc gacgctttcc gacacgagcg tgagcgccct
    10861 cgtcacgcgg atgcgcgcca tcacccccgt cacgccgacc ccgctgctca ttgacaccgt
    10921 tctcggctgc gagacccagc tcccgccggc cgtgatcgcg gaagcgcagg cagccgtctc
    10981 ggcgctgtac cggaccaccg cgcagcccta cggctaccag cactggcgcg actaccaccg
    11041 ccggttccgg gcccgctacg gcgtcggcgc gctggtgccg gtgatggacc tggtcgccga
    11101 cagcggtctc gggctgcccg ccgggtacgt cggctccgaa cgcggcgccg ccccgaaggt
    11161 gcgcacggac cgcgacgaac tgctgctcgc gctgatgcag caggtcttcc tggacggccg
    11221 cgacgaactg cggctgacgg acgacatggt caacgccctg gagaaggccg ccggaacgga
    11281 cgagcggctg ttcgtgcccc gggtggaggc cgccttcgaa gtcctctcac ccagcacccg
    11341 ggcactgtcc agcggcacct tcgaggtcct tctcaccgcg gtcccccgtc ccggcagcag
    11401 catggccggc cggttcgcgc acctcctgcc gtccgagcat cgcgacgcgc tcgccgccac
    11461 gtaccgcggg gccccggacc tcctcacagc gcagctcgtg ttcccgccgc gccgccaccg
    11521 caacgagaac gtcacccgca caccgcgact cctgccgcac ctcatcgagc tgtctggaca
    11581 ccaggagacc gacgagacga cgatccggct cgccgacatc gccgtcaccg cggacccgcg
    11641 cagcttccac ctcgtgcagc tcgccaccgg ccggcgcatc gacgtgcggg tcctgcacgc
    11701 cctggaaggc gggacacaga ccccaccact ggcccggttc ctcgccgaag tggccaacgc
    11761 gcggtgcgcc gcctacaagc cgttcgactt cggcgccggg gcccggctcc cgttcctgcc
    11821 ccgggtgcgc taccgccgta ccatcctcgc gcaggcccga tggctgctga tggccgacga
    11881 cctgcccggc cgcacagcct cgacgcggca gtgggaggac gccgtcgccg cctggcggga
    11941 ccggctgcgg gccccgacac aggtcgccat cgtcgagtac gaccagcgcc tccccctcga
    12001 cctgtcccac ccggttcatc gccaggtact gcgtgcccgc ctcgacaaca cccggcgtct
    12061 ggagctgcgc gaggtcccgg acgcggacgc ccacggctgg atcggccggg cacacgagat
    12121 ctggctctcc ctgagcaggt tgcagccgga caccgccgag tttccccgac gccgcaccgc
    12181 cacggcgccg gcccccggac ggcagcttcc gggcggcggc tacgtcctgc tcgcgcgact
    12241 ccacgcccac ccccgccgct acgacgagat cctcagccgc cacctgcccc gccttctcgc
    12301 cgcgttcggc gagatgacgc cggtctggtg gttctcccgg taccgggaga tggcccgtcc
    12361 ggacgccgac cagcacctcg acctcaccct gcacctgccg ccggacgcat acgggaccgc
    12421 cgcccagcac gtccgaaggt gggcggacag cctgcacgct gccggtctgg cagccgggct
    12481 cgtcctggct ccctaccagc cacagaccgg gcgcttcggc ggcgaggcgg ccatggacgc
    12541 cgcgcaccag gtgttcgcag cggactccgc cgccgccctc gcgcagatcc agctcaccga
    12601 gcgcaccgac accctcgccc cgcaggcact cgccgcagcc agtgccctgc acctcgtcgt
    12661 ccccctggcg ccggacatcg aggagtggtt ggtacgcaac ctcccccagg gcacgggtcg
    12721 actggaccgg gccctgcgta cccaggtgct cgaactcgcc gcgccgaacg gcgccgccgt
    12781 gctcgcggcc ctccccagcg gccccgaggt cacccaggca tggcaggccc gcgccgtcgc
    12841 cgtcgacacc taccggaaca ccctggccgg tcagcgtgat ccgctcgatg tcgtccggtc
    12901 gcttctgcac cagcaccacg tccgggcctt gggcgtcgat ccgcacgccg aggaaaccac
    12961 gatccggctc gtacgaaccg cggcccttca gcaccgaatg gcggtccgat gaccgcgcag
    13021 gcagtcaccg ccgcggtgct cctggagcag tacaccgccc agcttgcaca acccgctcct
    13081 ccgcccccgg acgagccatg ggcggttcag tccctcgccg acggtgcagc cggaatcagc
    13141 ctgctgcaca tcgagatggc cagccaccat gggggctcct ggcgccccgc ccataggtgg
    13201 atcaccgccg cagcggccgg gcacatcagt gcggccgaca ccgccgggct gttcctcggc
    13261 gccccggccc tcggcttcgc cctcaccacc gttccgccgt ccttccagca cttgtacgaa
    13321 ccggcgcgcc ggaccctgca ccggcacatc atcgacctga cccaccgccg cgtggacgcc
    13381 gcactcaccc gcatccgcaa gggcgacctg cccacgttcg ccgagtacga cctgttccac
    13441 ggcctcaccg gcatcggggc gtacctcctg cgaaccgacc ccgaagggcg cgccctggaa
    13501 cgcgtactga agtacctcgt tgccctgacc cgccctctcg cccctgacag ccgcaacctg
    13561 ccgggctggt gggtccgcca cgacccggcc cgcggtgaat ccccccactt tcccggcggc
    13621 cacggcaact tcggtgccgc ccacggcatt accggcccgc tcctcctgct ggcccaggcc
    13681 atgcgccgcg acatcaccgt cagcggccac cgcgaagcca tctggagcgt ctgcgaacac
    13741 ctcgacgcct ggtgccagct ccgtccctcc ggtccctggt ggcccgaaca catctcacgc
    13801 cgcgatctcg acctcgggcg cccgcaccac gacgcaccca cccggccgag ctggtgctac
    13861 ggcgtgacgg gcatcgcacg agccggacaa ctcgcaggca tcgccctgcg cgcccccgac
    13921 gtgcagacgt actacgaaga cgccctgtac cgcgccctga ccgaccccga acagctcgcc
    13981 cgcatcaccg actccggcct gtgccacggc tgggcaggca tctaccagac cgccactcgc
    14041 gccgccgccg atgctctcga cccccgcctt cagatcctca cgaaggggct cggcgacaac
    14101 ctcgcggcca acgcccgtcc cggcctgccc gagcccggcc tgatcaccgg tgacgccggc
    14161 tccgcactcg ccctcaccac ctacgcctca caacaaaccc cgatgaccgg atgggacgca
    14221 tgtctcttga tcaactgatc ttgacggagc cgagcccgat ccagcgcgcg gtcaccgctg
    14281 ccctcaccgg tctgccgatc gccgacaccg cagcccagta cgggatggaa cccacgacct
    14341 tgtccgacgc cgtggcggtc tacgaccaag ccggccggga agccctgtcc cgccacgaag
    14401 ccaacaccga ctggcgtcag gcgtatctcc acttcaccga ctgggcggac accgacacca
    14461 ccttcaccgc ctacgtactc cccgtgctcc gggaggcaga ggccgcaggg atcatcggcg
    14521 gctggtggta cacccgcaaa cacccgtgct ggcgcctgcg acttcgccta cagcccgagg
    14581 ccggaaccga actgcccctc aatgaacgcc tcgaccgtct cgtctccgac gggcatctgc
    14641 gacggtggtg gcctgggatc tatgagccgg agaccgcggc gttcgggggc gcggccggca
    14701 tgacggcggc gcacgcgcta ttcgtcactg acagccgaga ggcgccgcaa ctcgggcagc
    14761 gccaggacct ggccgtggga ccgcgggaac tgtccgtgct cctctgcacg atcatgatgc
    14821 gcgccgcggg cctggagtgg tacgagcaag gggacgtgtg gcaccgcgtc atcacgcaag
    14881 agcaccgatc ggccgtcagc gacgtcccgg cagaccggct cgacgcccgc gcccaggaga
    14941 tccggccgct gctcttcgcc gacagtgagg tcctgctgcg ccccggcgga ctgctggagc
    15001 ccgtcaccga gtgggtcgcc gtcttccgca gcacgggcca ggaactccgc cgcgccgttg
    15061 aggccgggac gctgcaacgc ggattgaggc aggtgctcgg ctatcacgtg atcttccact
    15121 ggaaccggct cggtctgtcc ctccggacac agagcatcct tgcctgggcg gccagggagg
    15181 cgatcctgca ccacaacagc cccacgtagc aggccgtgtc taactaatca ccgcagaatg
    15241 aggttcggag tcgtcgtcgc ggaagcagtc acgtgaccta gcccacgttc cgcttgtccc
    15301 gtggggctcc tgggcagggg caacccagga gcggtcgggg gtgacccgca tgaccggatg
    15361 ccgaggggga catctcggca tacgggacca tcggggtgtt ttccggccgc tcgttagact
    15421 gagccgaccg cagtactgac taacgaggag cgcacgtggg ccttgtcgtg cagaagtacg
    15481 gaggctcctc cgtagccgat gccgagggca tcaagcgcgt cgccaagcgg atcgtggaag
    15541 ccaagaagaa cggccatcag gtcgttgtcg tggtgtccgc gatgggcgac cagacggacg
    15601 agttgatcga tcttgccgag caggtgtcgc cgatgcctga cgggcgcgag ttcgacatgc
    15661 tgctgaccgc cggggagcgg atctccatgg ccctgctggc gatggcgatc aaaaacctgg
    15721 gccacgaggc ccagtccttc accggcagcc aggcaggggt catcaccgac tcctcgcacg
    15781 gcaaagcgcg catcatcgat gtcacgcccg gccgcatccg caccgccctc gacgagggaa
    15841 acatcgcgat cgtggccggt ttccaggggg tgtcccagca gaagaaggac atcaccaccc
    15901 tgggccgggg cggctccgac accaccgcgg tcgcactcgc ggcggcgctg gacgccgagg
    15961 tctgcgagat ctacacggac gtggacggtg tcttcaccgc cgatccccgt gtcgtaccga
    16021 aggccaggaa gatcgagagc atcgccttcg aggacatgct ggagctggca agctccggct
    16081 cgaaggtgct gctgcaccgc tgcgtcgagt acgcacgccg atacaacatc ccgatccacg
    16141 tacgctcgtc cttctcggga ctcaagggca catgggtcag cagcgaagtt cgaggggacc
    16201 agacgatgga gcaggcgatc atctcgggcg tcgcccatga cacgtccgag gcgaaggtca
    16261 cggtcgtcgg cgttcccgac aagccggggg aggccgcgac gatcttcagg accatcgcgg
    16321 actccgagat caacatcgac atggtggtgc agaacgtctc cgccgcgtcg accggtctca
    16381 ccgacatctc cttcacgctg ccgaagtccg agggccgcaa ggccatcgac gcactggaga
    16441 agaccaagcc gaaggtcggc ttcgaatcgc tgcgctacga cgaccagatc gccaagatct
    16501 ccctggtcgg agccggtatg aagaccaacc ccggcgtcac cgcggggttc ttcgaggcgc
    16561 tctccgacgc cggagtgaac atcgagctga tctcgacatc cgagatccgt atctcggtgg
    16621 tcacccgcgc cgacgacgtc aacgaggccg tccgcgccgt gcacaccgcc ttcggtctcg
    16681 acagtgacac cgacgaggcg gtcgtttacg ggggctccgg acggtgacct tctcctacgt
    16741 cggcggcgta cggtgacggc atgaccgtaa ggccgacgct cgccgtcgta ggagccaccg
    16801 gtgccgtcgg caccgtgatg cttcagatcc tgtcccagca cgccgacatc tggggcgaga
    16861 tccgtctcgt cgcctccccg cgatcggccg gccgcaagct gaccgtacgc ggcgaggagg
    16921 tcgaggtcgt cgcagtcagc gaggaggcct tcgacggcgt cgacatcgcc atgttcgacg
    16981 tacccgacga ggtcgccgcg cgctgggcgc cggtcgccgc ggccaagggc gccatcgtcg
    17041 tcgacaactc cggcgccttc cgcatggacc cggacgttcc cctggtcgtc cccgaggtca
    17101 atccgcacgc ggcccgccgc aggccccgct cgatcgtcgc gagcccgaac tgcgcgaccc
    17161 tctcgatgat cgtcgccatc ggcgccctgc acgccgaatt cggactccgc gaactcatcg
    17221 tctcctccta ccaggcggtg agtggcgcgg gccgggccgg tatcgagacg ctgcgctcgc
    17281 agctcgccat ggtcgccggc accgaactcg gcacctcacc cggcgatgtc cgccgctccg
    17341 tcggcgacga aacgggcccc tttcccgcac ccctcgcgct gaacgtcgtg ccctgggtgg
    17401 gctccctccg tgaggacggc tggtcctcgg aggagatgaa ggtgcgcgac gagtcccgca
    17461 agattctggg cctgcccggt ctgaaggtga cggcgacctg cgtccgcgtt cccgtggtca
    17521 ccacccactc gatgaccgtc cacgcccgct tcgaggacga ggtcaccgtc gacaaggcgc
    17581 gcgagatcct cgccaccgca cccggcgtgg tcctcttcga cgacccggcc gaaggtgaat
    17641 tccccacccc cgccgatgtc gtgggcaccg acccgacgtg ggtcggccgg gtacgccgct
    17701 ccctggacga cacagccgca ctcgaacttt tcgtctgcgg cgacaatctg cgtaaggggt
    17761 ccgccctcaa tacggcacag atcgccgagc tgatcgcttc ggagttcacg gcctaggcgg
    17821 cctcgacggc ctaggcggcc tcgacggcct cgacggcctc gacggccttg ggggattcgc
    17881 cgactgtggt ggacttggtg gccttgacgg ctgtgccggt tgcggcggcc gtggtggctt
    17941 tgacagcttc gccagctgtg gcggctttga cggcgtgggt ggcgtgggtg gcgtaggcga
    18001 cgtagcagat accgtcccgc gcgttcccgc gcgttccggc cgtttccggc atgtcggtgc
    18061 aggcgggcgc aggtcaccca aggttccgcc ctggcgcatg tatctatgga ggacctgtgt
    18121 aacttgtgtg gtcaagtggg gtgctcaatc cagttgatca ggtccgtcgg ccgccggagg
    18181 attcgtctta cgtgcctctg caaccgtgtg acggtcggga agcgtctttg cggtcgccca
    18241 cacgcggcgc ggcagctggg gcatttgtgg tcatagggga agagctaatg cgcatgaggg
    18301 cattgagggc atcttcggac gggatgacgg acccgtacaa ccccggaggg gggcggcgag
    18361 tccaactggc gtggcagagg ttctcgaatt caccgcggtc caggtgccag gcgcagtcct
    18421 gcacccactc cgtcgtcccc gaatgcccgg tgctcccggc agtatgccgg tgattgcccc
    18481 tatgcccgtg acacgaccga cggctcgaat tcccggacag cgcgagggtg ctgacgacac
    18541 gacacccgac tccgcgggga ccacggtcga ccacctcacc gagacctacc gcacccacta
    18601 ccgctccctg ctcggcctcg ccgcgctgct gctcgacgac accgcttcct gcgaggacgt
    18661 cgtgcaggag gcgttcatcc gcgtccactc ggcccgcaaa cgggtcaggg atcccgagaa
    18721 gacgctcgcc tatctgcggc agaccgtggt caatctgtcc cgctccgcac tgcgccgtcg
    18781 catcctcgga ctgaagctgc tctccaagcc gatgcccgac atggccagtg cggaagaggg
    18841 cgcgtacgac cagttggagc gtgacgcgct catcaaggcg atgcgcggcc tccagcggcg
    18901 ccagcgggaa gtactcgtcc tgcggtactt cgcggacatg accgaagctc aagtcgccaa
    18961 aaccctcggg atatcactgg gctcggtgaa ggcgtacggc tcgcgcggta tcgcggcact
    19021 gcgcgtcgtg atggaggcgc aggcatgagc gcggacaacg cggataaccc ggatagcgcg
    19081 aatagcgcgg acgacgcgaa tagcgcggac gacgtgagta agccagatcg gccgcggcgg
    19141 tcgcagcccg cggggcggtc ggaccagccg gatcggccgc aggggtcgga tcagccgggg
    19201 cggtcggatc gaccggggcg gccagagcgg cgagaacagc tagaagagcc agaacggcaa
    19261 ggcgagcggg atgaacacga gtggtgtgaa ccggtgagcg gcgaatccgc gatcggcgcg
    19321 cacacttcgg agggcgagag cggcgtgagc cgtgagagcg gcgaggggcc cgaagggccg
    19381 gggcccaccc cgctcggccc ctccggtgtg gacgaggact cgctgcgcga cctcctgcac
    19441 cacgccgtcc agggcatcga accgtccgac caggcactcg accggctcca gcgggcggtg
    19501 cccgcgcgca ggacccgcaa gcgccaggcc gtcgtcggtg tcgccgctgc cgcgatcctc
    19561 atcggtacgg ccgtccccgc gctggtgcac gtcaccgcgg aaccgagcgg cggtcacgac
    19621 cacccggcca tagcgggtga caccgagcgg acggagatcg gcgcgggcct ggaggacgac
    19681 cggcacggcc gggcgcccgg cggtcacagc ccctccgaca agtcccccgg ccacaccaag
    19741 ggaggccgcg aggacagcga ggagaccaag ggcgccaagg acaaggacac caccgagccg
    19801 gccgacggcg ccgagcccac gggcagtacg gccgcctcgg gtccaccgga gtgtgatcca
    19861 ggccagctgg gcgcacccaa ggcgaccacc agccagcccg acaaggacgg caaggtctac
    19921 ggcaccttca agatcgccaa cgtctccggc aagaagtgtg tgatcgaggg cgagggcagt
    19981 gtcgccgcgg ccgccctggg cgcggccgac cccgccaaga tcgccgtggt cgagcacatg
    20041 ccgggcgacg cggccaccaa gctgcccccg ccctcgcgtg cggagcgcgc gctgatgctt
    20101 gagccgggta cgtcgtacga ggtgcgcttc gcctgggtgc cctcggcgga ctgccacacc
    20161 gacgaccacc ccggcgaagg caccggctcc gtgtcgcccg acccgagccc gtcgggcgac
    20221 acggagatcg accgttcggc ctcgcccgat gtgaagagcg gtaccaacga cgtctccccg
    20281 cagctcctgc gcgaggccga cggcgatgag gacggcagcg tgtcgatcag ccacaccgcg
    20341 gagcggggcg gtcctacggc ggacacgacc atccccgacg cgtgcgcggg cacgatctac
    20401 cggacgggcg tactccagcc gcagtgagcc ggggcggtcg cgccgtgcca gtcccgagtc
    20461 cggcaggggc gggtcgcgcg gtgggacagt cccggtggcc gggacagcct cgccggactg
    20521 ccggactgcc ggactgccgg actgccggac tgccggactg ccggactgcc ggagtgggcc
    20581 gctcagcccc gcgtgtccga accggaccgt gccacagggc cgccgaccgt agcctcgtcg
    20641 gttggggccg cgtcggcagc agtcccgccg gccgaagccg aatccggagc cgcatccccg
    20701 cccgccgcag aggcaggcgc agaggcaggc gcagccgcag cggcaggcgc agggaccgaa
    20761 ccgcccgaac cgcccgaccc cgtcacgaac atgaagggct ccggctccgg tgcggggata
    20821 ccgagcgcct cgtcccgcgc cgcctccgcc tcgcggcgca gcagcctgaa ccacatgaag
    20881 accacgaacc cggcgaagac gaaccactca ccggtgtacc cgaggttctg gaaggccttg
    20941 aggtcgagcc cggtgtcggg cggtgccgcc gcgggtaccg cccgcatccc cgagtcaccc
    21001 ttgtcgaggg tgatccacgc gtcgtagacg tcgtaggagg tgaggttcac cagcgaagcc
    21061 gcgctgatca tgccgagttg gccttggggg agtccgcccg aggtgctgac cccgtcggtg
    21121 ccggtgttct ccgaggactg gagggcgccg cgtacggaga cctcaccggt gggcagagcg
    21181 ggcgccttgg cggcggaggc gtgaccgggc acccagccgc gtacgaccgg cagaaccttg
    21241 cccttgtccc cgccgccccc gtcggtacgc agcagaccga ggacgtagaa gccctgcttg
    21301 tcttcgacga cccggccggg cacgaggagt tgtctcacgt accggccgct ggtctcggcc
    21361 gtgcggcccg aggtcttctg gtcgacgggg agcaggttcc tgaggggcgc ggcccgctgg
    21421 tgcgccgcct ttgcgggggc ctgttcggcg gagtgctgcg catccatccg gtcctcgaac
    21481 cggctgagct gccaggaccc catgaacacg cagaagggga tcgccagcac gacgaatacg
    21541 ttgattcccc accaccgggg agtcagcagg aaccggtaca cacccccacg gtacggggcg
    21601 gccgacctta tgaggtcggc ggccccgccg tgtcggcgcg cgcgacgccg cccgtgcggg
    21661 ggcgtacgcg cgggcggacc gctcccgaag gggccgcccc tcgccggggc cgctcccgaa
    21721 cggggcgacc cccggtaggc cggggtcgcg ccccgcgccc cccggccccc gcctgccggc
    21781 tacggcctcg gctcgcgccc gagctgtcgc tgagcgaaat cgatctccag ccgtacctgc
    21841 ttgatccgct cgcccacgac cagcgagccg tgcccggcgt cgtagcggta gacctcgtgg
    21901 gtggcgcccc gcgcggcgag cctgtcgacg tagttctcga cctgcttgat cgggcagcgc
    21961 gggtcgttga ccccggccga gatgtagacc ggcgcccgca ccgcgtcgac gtacgtgatc
    22021 ggcgacgacg cctcgaaccg ctcgggcacc tcctcgggcg taccgcccag cagcgtgcgg
    22081 tccatcgcct tcagggcctc catctcgtcg tggaaggccg tcacatagtc cgccacgggc
    22141 acggcggcca ggccgactgc ccagtcgtcc ggctgcacac cgaggccgag gagcgtcaga
    22201 tagccgcccc aggacccgcc ggacagcacc agcctctccg ggtcggcgag accggaggcg
    22261 accgcccagg cgcgtaccgc gccgatgtcc tccagctcga tcagcccgac acggtgcttg
    22321 agcgcgtccg tccaggcgcg gccgtagccg gtggagcccc ggtagttgac ccgtacgacg
    22381 gcgtaaccgt gatcgaccca cgcggccggg cctgacgcga aggcgtcact gtcgtgccag
    22441 gtgggcccgc cgtggatctc gaagaccgtc gggaaggggc cctcgcccgc tggccgctgc
    22501 acgagggcgt ggacccggcc gccggggccc tccacccagg cgtcctcgac cggcacggaa
    22561 ccgggtgcct tcatgccggg aggatcgagt acgacctcac ccgaggtgga ccgtacgacg
    22621 ggcggcaggg cggcggacga ccacaggtac tcgatggaac cgtcggggcg cgcggtcgcc
    22681 tccgacacgg acccggcggg cgtctcgacg cgcgtgaggg aagcgtcggc gaggtcgtag
    22741 cgccacagct cgctgcgcgc ctcgaaactg tgcacgacca gcagcgcctc gccgtccgga
    22801 taccactccg cgctcacgtc gccgggcagc ccgaagacca gctcgcgctc cgcaccggtg
    22861 gcgacgtccc acaccagcgg ctcccagcgg cccctgcgct ggtgcccgac gaggagacgc
    22921 gtgtcacccg cgaccggcgc gaagccgagg acggacaggc ccagctcctt cgtcccgccc
    22981 tcggagtcgt cgagctccgc gaccgtctcc ggggcgctct ccgagccttc gggcgcgggc
    23041 cgcagcaccc gcagcgccgc agcgccgagt gcatcgcgtt gtcgtgctcg gtgtgctcga
    23101 tcaccacaag ggaaccgtcg tgcgagaggt cgccgacccc ggccgactcc gggtggcggt
    23161 agatctccac gggggcgccg cccgcgccgt gtacgacgtg gaccgtcgag ccctcgtcgt
    23221 cggtggagga cccgacgacc accgtggccc cgtcccgccc gatggcgagc ccggccgggt
    23281 aggccggggc gagcccctcg acggcgggca cgtcgtcgcc gccctcgaac ggctgccgca
    23341 tccagacacc gaactcgtca ccgtcggtgt cgtcgaacca ccagatccac cggccgtcgg
    23401 gggagaggac cccgtcggtg gtcccgttcg cacggtcggt gacctgccgt tgttccccgg
    23461 tggcccggtc ccaggcgtac agctcgtacg tccctgtcgc gttggagacg aacaacgaac
    23521 ggtcgggggc gtcctccgcc cactcgggca gggagacacg gggagcgcgg aaccgcttct
    23581 cccagtcggg catggcttcg gcgtcactca tgcccccatc caacccgatg gccgtccggc
    23641 cgttcaccgt tcccccggag gggcgacggt ggggacggag gggcgacggt ggcggggtgg
    23701 gtgaacaccc cgccacctgg cgggaaaccc cctcctggag ggggcgcctg gaagcggctc
    23761 ctggagcgtg ctgggctcca ggagcgtgct ggtctcctgg agggtgctgg gctcctagag
    23821 cgtgctgggc tcctagagcg tgcgctcgac gcggtccgcc atcagcttga tgaagcggga
    23881 cgggtcgctc agctcaccgc cgtcggcgag cagcgccatg ccgtgcagca gctcggcggc
    23941 ctcggccaat ccggtgccgg actcgtccgc ggcatcgtcc gcggtcccgt ccgcggcctc
    24001 gtccttcgcc gcgtccccgc cggaaggcgc ctgctcggcg tgggccttcc gcaagccgct
    24061 gaccagcggg tgggtcgggt tgagttcgag gatgcgcttg acctcgggca cctcctggcc
    24121 catggcgcgg agcatgttct ccagcgccgg ggtcacatcg ctgttgtccg agacgatgca
    24181 ggcgggcgag gaggtcagcc tggaggagag ccgtacctcc ttcacctcgc cgccgagctg
    24241 atccgtcatc cagctgagga gacccgcgaa gtcggcctgc cgctgctcgc gttcggcctc
    24301 gacctccttc ttctcctcct cggtgtcgag gtcgacctcg cccttggcga tcgactggag
    24361 gggcttgcct tcgaactcgg gcacggactg cacccagacc tcgtcgaccc ggtcggtgag
    24421 gagcaggacc tcgaacccct tggccttgaa ggcctccatg tgcggcgagc tctccatcgc
    24481 cgtacgggag tcgccggtca tgtagaagat gtgctgctgc ccctccttca tccgctccac
    24541 gtactccttg agagtcgtgg gggcggcggc gtcgtgggtg gaggcgaagg acgagacgcc
    24601 gagaatggcc tcgcggtttt cgaagtcgct gagcagaccc tccttgagga ccgcgccgaa
    24661 ctccttccag aaggtcgcgt aacggtcggc gtcacgggac atcatgtcct tgaccgtcga
    24721 cagcaccttc ttgacgagac ggcggtgcat caactggatc tggcggtcct gctgaaggat
    24781 ttccctggat acgttgaggg aaaggtcggc cgcgtcgacg acacccttga cgaagcgcag
    24841 gtagtccggc atcagcgctt cgcagtcgtc catgatggag acgcgcttga cgtagagctg
    24901 aataccgcgc ctgaacccct gcatgaaaag gtcctgcggg gcgtgcgagg gaatgaaaag
    24961 aagagcctgg tactcgaagg cgccctccgc ctgaatacgg atggtctcca gcgggtcgtt
    25021 ccagtcgtgg ctgacgtgct tgtacagctc gctgtactcc tcgtcggtca cctcgctctt
    25081 cgaccgcgcc cagagggcct tcatggagtt gaccgtctcc acctcggcct cggccttgtc
    25141 ctctgccgcc gactcctcgc cttccgcggc cccgggctcc ggctcctggg cggccggctt
    25201 ctcgacggc
//
