LOCUS       NZ_CP045178            22533 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3038760
            Orig. end                         :: 3061293
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22533
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="14"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22533
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22533
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12533](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12533
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(245..616)
                     /locus_tag="GBW32_RS12865"
                     /old_locus_tag="GBW32_12855"
     CDS             complement(245..616)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834898.1"
                     /locus_tag="GBW32_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12855"
                     /product="DUF2255 family protein"
                     /protein_id="WP_077969489.1"
                     /transl_table=11
                     /translation="MKTWNGSQLERIGAAEELELRSERGDGTLRNPVTMWVVRAGDHVY
                     VRSVKGQDGPWYRGTRTREQGDVEAGGVRQDVTFNEADPREYAGVDAAYREKYGHYTTI
                     VDHVLTDRARTSTLRLEPR"
     gene            complement(810..1658)
                     /locus_tag="GBW32_RS12870"
                     /old_locus_tag="GBW32_12860"
     CDS             complement(810..1658)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049258.1"
                     /locus_tag="GBW32_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12860"
                     /product="aldo/keto reductase"
                     /protein_id="WP_077969488.1"
                     /transl_table=11
                     /translation="MRTVTLNNGVEMPVLGFGVYQIPPDQAEQAVTDALAAGYRSLDTA
                     ASYGNEEAVGRAIKSSGIAREDLFVTTKLWVSDAGEDQARRGFDTSLRKLGLDYLDLYL
                     IHQPFGDVYGSWRAMESLHRDGLARAIGVSNFHGDRLVDLIDHNAITPAVNQVETHPFH
                     QRAADQELMSERGVQIESWGPFAEGRNHLFTDPVLVAVASAHDKSVAQVVLRWLIQRNV
                     VVIPKSVRPERMAENLDVFDFDLRSDEMAAIAKLDTGESLFFDHRDPAMVSRLGNATLD
                     S"
     gene            complement(1705..2682)
                     /locus_tag="GBW32_RS12875"
                     /old_locus_tag="GBW32_12865"
     CDS             complement(1705..2682)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530361.1"
                     /locus_tag="GBW32_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12865"
                     /product="aldo/keto reductase"
                     /protein_id="WP_077969487.1"
                     /transl_table=11
                     /translation="MQHINLGDLRVSRIGLGAMGMSHGYTDSGTDEAESVRTLHRALEL
                     GVTLIDTAEIYGPYVNEELVGRALKGHRDEVVLATKFGLVSHGGAGAWTLDSSPDNIRS
                     AVEGSLRRLGTDHIDLYYQHRVDPNTPIEETAGAVGELIGEGKVRAFGLSEAGPDTIRR
                     AHAVQPVSAVQSEYSLWTRGLEERVLPVLRELNIGLVPFSPLGHGFLTGTIRSTDEFKE
                     GDFRYGNPRFSGENFQRNLRIADEVQAVAEQAGATPAQVAIAWLLAKGDDIAPIPGTKR
                     VARVEENTAAESLRLTDEQIDRLSSLPPAAGETHPEGARQMLER"
     gene            3205..4230
                     /locus_tag="GBW32_RS12880"
                     /old_locus_tag="GBW32_12870"
     CDS             3205..4230
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141226.1"
                     /locus_tag="GBW32_RS12880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12870"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077969486.1"
                     /transl_table=11
                     /translation="MAPEQSSGDELGRFLRARRTQTSPRSAGLTPGPGVRRTPGLRREE
                     LATLAGVSIDYYTRMERGKETRPSPAVVDALARALRLDDAEHQHLRDLAVRAARYAHPA
                     PEPSRTVRPHLELLLETVRPNPAYVVSRSMDLLAHNPGGLALYAGIADWPARQRNLARY
                     LFLHPAAREVFGDWENQIRGCVARLRALAGIDPDAPDLTRLVGELLLKSPDFAGLWERY
                     EVTGRKYTTKTFHHPRVGRLTVGFQGMDLEGTPGQRMVVYTAEPGTPDHDAMVLLDMTA
                     PEQQTSSASARSAASTEPVASTKPTGPADATGSTGATGPTGPTGPTGPISEQARERGRR
                     D"
     gene            complement(4472..5794)
                     /locus_tag="GBW32_RS12885"
                     /old_locus_tag="GBW32_12875"
     CDS             complement(4472..5794)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846398.1"
                     /locus_tag="GBW32_RS12885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12875"
                     /product="ROK family transcriptional regulator"
                     /protein_id="WP_227025490.1"
                     /transl_table=11
                     /translation="MPVLRGHNTALVLDLLRVSGETGISRLELAERTGLTPQAVSKITA
                     RLRTSGLATEAGRRASTGGKPRTVLRLVPTAAYAVGVHLDRDELRTVLVDLAGTTVRER
                     TAPLDLGAGADAVIEATAGEVELLLGAVPGDRGSVDGGGSADDGAGRPGQPGRLTPGPR
                     GRVLGVGVGVPGPLDHREGVLHRVTGFPDLDGFPLRDALAARLGLPVTVDKDTNAAALG
                     PALGMTDIAGPVFGAGEGTRPGSGPGEGVRPGSGPGEGVRPGSGSSSGSGLRLGPGGGS
                     FAYLHLGTGLGAGLVLGGLLHRGARTGAGEFGHQVIQLDGPLCGCGDRGCVEALCLAAV
                     GRGDPDEAARVLGTAASNLVGLLDIDLVLLGGRTIAAESERYVAGVEAVLARRAGREGA
                     GPAVPVRLAPGGVRGIADGAAQLVLAPVFGRADTAGATGLA"
     gene            6217..7407
                     /locus_tag="GBW32_RS12890"
                     /old_locus_tag="GBW32_12880"
     CDS             6217..7407
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886654.1"
                     /locus_tag="GBW32_RS12890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12880"
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_077969484.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 9.3e-25, bitscore:
                     86.3, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 4.8e-27,
                     bitscore: 93.1, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLTGTDPEPEAGSGSRPPLRVALIGYGLAGSVFHAPLIAATDG
                     LVLDTIVTSNPERREQALAEFPDVRFAGSPDELWADAARIDLVVVATPNKTHVALAKAA
                     LGAGLPVVVDKPVAGTATEARELDALARERGLLLSVFQNRRWDNDFRTLHKLLAHGDLG
                     DVYRFESRFERWRPHPKGGWRESGDPAEIGGLLYDLGSHVVDQALTLFGPAASVYAESD
                     VRRAGAEADDDTFIAITHESGVRSHLYVSATTAQLGPRFRVLGSTAGYVKYGLDPQEAA
                     LREGRRPAKGGAWGEESPELWGRLGAGESPLSGGGEPVRTLPGDYPAYYAAVAATLRGD
                     GPSPVTALEAAATLDVLEAARRSADEGVTVPLGAEPPARADTRIPTKSPTIEETTT"
     gene            7404..7955
                     /locus_tag="GBW32_RS12895"
                     /old_locus_tag="GBW32_12885"
     CDS             7404..7955
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565619.1"
                     /locus_tag="GBW32_RS12895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12885"
                     /product="heme-degrading domain-containing protein"
                     /protein_id="WP_077969483.1"
                     /transl_table=11
                     /translation="MSGSSGGTSGTKGAGGPDVPELEAQEKRLVLKRFTYENAWALGSL
                     LVELARERRAPVAIDIRRGAQRVFHAALPGSSADNDAWIERKRRVVERYGESSYLVGSR
                     FRAKGTTFEDSSRLDQDRYAAHGGAFPIAVEGAGVVGVVVVSGLPQIEDHALVVAALER
                     FTAQRSVSDAFVSGSSGDGA"
     gene            complement(8176..8580)
                     /locus_tag="GBW32_RS35660"
     CDS             complement(8176..8580)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS35660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_179120216.1"
                     /transl_table=11
                     /translation="MTDAEWVVVRPLPLPLPLPPVPLPVPGWMRGRGAGGVLPPGDAGR
                     CALSGGQRDQVADPASDLPPRDRVCAFFRRWRERALVRVFPDRLRGRVDETAGRDAEPT
                     AVVIASRSVKEDAVVGEDSRGFDGGKLALF"
     misc_feature    complement(8437..8439)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8970..9590
                     /locus_tag="GBW32_RS12905"
                     /old_locus_tag="GBW32_12895"
     CDS             8970..9590
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="GBW32_RS12905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12895"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_077969482.1"
                     /transl_table=11
                     /translation="MSACDQPLPEKSPDQRPATGDTLSGASAAPDECHDVRAHFDAVFT
                     QLLPALYRRSRSLSAARDLAEDAVHDTYLKLSARPRSLLGHSHPYAYACRALLSVTRDA
                     WRRERRMVPTGELEAGPTGFEEGPDARLAELMALRLLRSLTERQAQAVILVDIEGHTLD
                     EAARRLGLHRGTVAQLRRRALESLRDKTEAESKDLGPAHRPRG"
     misc_feature    9798..9812
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.23"
                     /tool="antismash"
     gene            9821..9958
                     /locus_tag="GBW32_RS12910"
                     /old_locus_tag="GBW32_12900"
     CDS             9821..9958
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /locus_tag="GBW32_RS12910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12900"
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_107502901.1"
                     /transl_table=11
                     /translation="MENTDVYEPPVLVEAGGFMELTLGADKGGVTDFAGWFDIFSDFQG
                     "
     CDS_motif       9821..9889
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MENTDVYEPPVLVEAGGFMELTL"
                     /locus_tag="GBW32_RS12910_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9890..9958
                     /prepeptide="core"
                     /locus_tag="GBW32_RS12910_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-0.10"
                     /molecular_weight="2319.4"
                     /monoisotopic_mass="2318.0"
                     /core_sequence="GADKGGVTDFAGWFDIFSDFQG"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MENTDVYEPPVLVEAGGFMELTL"
                     /leader_location="[9820:9889](+)"
     gene            10001..11842
                     /locus_tag="GBW32_RS12915"
                     /old_locus_tag="GBW32_12905"
     CDS             10001..11842
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.5"
                     /locus_tag="GBW32_RS12915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12905"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_077969481.1"
                     /sec_met_domain="Asn_synthase (E-value: 6.9e-32, bitscore:
                     110.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGLSMMRGSWFAVLPDREAAEEAAARLRAWAGQEVRHASGRPWL
                     LGCWEPSEVTTARAGGHAAAVLGRHVLTEAELAARLNTVVTTGDVEHFADEWAGSFHLI
                     LSVHRQIRVRGTASATRRVFTVRQKGATIASDRADVLAALSGAAVDVRRVAMRLLSPQV
                     AHPFGEHSLWTGVDYVPADQELVVEPGGRTHLARWWSPPEPVLPMAEGAERVRAALRAS
                     LNTCKGAASVLSCDLSGGMDSTSLCFLAVGKPSRLVTVRREAADPGNDDAMWAEAAAGH
                     LSDGHHLTLRDGEIPAWYADVATPADDREEPYKDIRGRAYRRAMARHMANLGAQTHVCG
                     VGGDELFGSSPAYLHDLLRLRPLLTLGRVAAHRATARWPLTTALRALSQRGTFDDWVKG
                     AADGLSAAPRRVQLDWMPRLRLPPWATPEAAELVRGVLHEAARCSWEPLSPSRVQHQTA
                     QHVRTAGMSVRQIDRLMRSTGAGMAAPFLDDQVITAAMSVRLEDRFSATSFKPLLAQAV
                     AAHMPSALLERRTKGDFSFDLHQAMHRRRADLLELTDTLRLGELGLIDAAALRQAVLTP
                     HPRGMTLTPLEATFSCESWLRGLDSRSLHLAHPKGHT"
     gene            11842..12105
                     /locus_tag="GBW32_RS12920"
                     /old_locus_tag="GBW32_12910"
     CDS             11842..12105
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545010.1"
                     /locus_tag="GBW32_RS12920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12910"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_077969480.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.9e-21,
                     bitscore: 74.0, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.4e-13, bitscore: 48.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLALRSDVSATDGDFDMVLLNECTGRYWYLNATGAFILRLFLDG
                     SERAEVARRAAERFPVSEEKASQDIETLVSSLCTADLVKVKA"
     aSDomain        11848..12096
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_GBW32_RS12920_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.20E-23"
                     /identifier="RREFam002.1"
                     /label="GBW32_RS12920"
                     /locus_tag="GBW32_RS12920"
                     /protein_end="85"
                     /protein_start="2"
                     /score="74.0"
                     /tool="antismash"
                     /translation="LALRSDVSATDGDFDMVLLNECTGRYWYLNATGAFILRLFLDGSE
                     RAEVARRAAERFPVSEEKASQDIETLVSSLCTADLVKV"
     gene            12102..12533
                     /locus_tag="GBW32_RS12925"
                     /old_locus_tag="GBW32_12915"
     CDS             12102..12533
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140854.1"
                     /locus_tag="GBW32_RS12925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12915"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_077969479.1"
                     /sec_met_domain="PF13471 (E-value: 8.4e-26, bitscore: 88.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTHMVPESGIRLPMRKQVPARTAVAAARLLALLPPRHIRRALNL
                     LRRGARPAGFQDALTARTAVISVSTRCAGGEGCLQRSLATAILCRMAGHWPQWCAGVRT
                     QPLRAHAWVEVEGVPVGEPYPAHYFQLLVHVPEVPSERG"
     gene            complement(12634..13494)
                     /locus_tag="GBW32_RS12930"
                     /old_locus_tag="GBW32_12920"
     CDS             complement(12634..13494)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990503.1"
                     /locus_tag="GBW32_RS12930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12920"
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="WP_077969557.1"
                     /transl_table=11
                     /translation="MKLLRVGTAGSERPALLDAEGVLRDLSGLIGDIDGALLADTTGAL
                     ARVREAERAGDLPEVDTVGLRYGAPLAGVGKVVCIGLNYHDHAAETGAPAPPEPIIFFK
                     AADTVVGPDDTVLVPRGSTRTDWEVELAVVIGKTARYLDTSEDGLAHVAGYAVANDVSE
                     REFQIERGGTWDKGKNCETFNPLGPWLVTADEVPDPQALALRLWVNGELRQNGTTADQI
                     FPVGEVVRYVSQFMTLYPGDIINTGTPAGVAMGQPEPKPYLRAGDVVELEVEGLGRQRQ
                     EFKDA"
     gene            complement(13959..14774)
                     /locus_tag="GBW32_RS12935"
                     /old_locus_tag="GBW32_12925"
     CDS             complement(13959..14774)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555808.1"
                     /locus_tag="GBW32_RS12935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12925"
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="WP_077969478.1"
                     /transl_table=11
                     /translation="MSAFFSAFASLVAHFADLLHPLFHDASTAVAIILFTTCVRLLVHP
                     LSRASARGQRARAKLQPQIAELRKKHVKNPEKLRQAVLELHAAEKVSPLSGCLPSLFQI
                     PAFMLLYHLFSSSSIGGDPNSLLGHTLFAAPLGDRWKDALGNGGPFGDSGLVYVGIFAV
                     VIVVATFNYRRTKRQMASAASPLAQVSGSTRTDKAGGTEAPGTEVPGIGAITKLMPLMS
                     FMTLFSVAVVPLAAALYLVTSTAWSAVERAVLYRDMPAATPAPALSPAA"
     gene            complement(15223..15537)
                     /locus_tag="GBW32_RS12940"
                     /old_locus_tag="GBW32_12930"
     CDS             complement(15223..15537)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121136.1"
                     /locus_tag="GBW32_RS12940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12930"
                     /product="DUF6412 domain-containing protein"
                     /protein_id="WP_077969556.1"
                     /transl_table=11
                     /translation="MAGRWTTSRSVGLVLFLLAGVLLTDGGGLSGAVALAATATAAAGS
                     ALVACSVIAARCAPAVPPTRVRTAIRDREHRTAFLPQRDPDASGRPRPRAPGRRLLTAA
                     "
     gene            complement(15591..16121)
                     /locus_tag="GBW32_RS12945"
                     /old_locus_tag="GBW32_12935"
     CDS             complement(15591..16121)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138930.1"
                     /locus_tag="GBW32_RS12945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12935"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_077969477.1"
                     /transl_table=11
                     /translation="MALGKDYTAQQCSIARTLEIVGERWTLLVVRDALYGVRRYNDFLV
                     HLGIPRAVLAARLHSLTEAGVLDKRRYQESPPRDEYVLTDAGIALWPVIRALGAWGRDH
                     VGDSLPPREFRHFACGTRLGSYGECPVCAVPVPVPDVEMIPGPGLDPDPSDPVGRALLA
                     PRRLLQPVVTDPV"
     gene            16248..17741
                     /locus_tag="GBW32_RS12950"
                     /old_locus_tag="GBW32_12940"
     CDS             16248..17741
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975946.1"
                     /locus_tag="GBW32_RS12950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12940"
                     /product="MFS transporter"
                     /protein_id="WP_077969476.1"
                     /transl_table=11
                     /translation="MTGTGAGTDAGTGTGTAVGTAARVRAGTAADAGAGAGGTAGASRT
                     TVALTLISAVTAIALMNYTTPMLTLPGMIASFGTPLSAQAWLLNGTPLGLAALLLVAGS
                     LADDYGRRRLFLTGTLALGVTTLLGSLATTTLLFTLARAAQGMASAAIITSGLGLIVHA
                     FPPGHTRIRATGIWGACVSGGIALGPLLAGVTAGLGWRAVYVVLGAAALLAAAFVPRVL
                     KESRAPRPGRPDLLGALTLGLASVSLLAALTLGRDGWLRAPVWGLLAATVVLAGAFVLV
                     ERRGEAPMLDLGLLRHRGFLAATSGALFTGVAVIGLFSYLAALIQSTLGLGTLGVAWLF
                     VLWAGTGFVVALQARRLAGRVGARHQLALGFVLSAAGVLTMLGALNAGSWPRLVPGLLI
                     AGVGNGLLNAALPQLSVESVEPQRAAMGSGANNTARYIGSSAGVALSIAVATSATGETP
                     AKALAHGADHALLVSAALALLGALVTLALRDRRDPVARR"
     gene            18055..19059
                     /locus_tag="GBW32_RS12955"
                     /old_locus_tag="GBW32_12945"
     CDS             18055..19059
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931020.1"
                     /locus_tag="GBW32_RS12955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12945"
                     /product="SEC-C domain-containing protein"
                     /protein_id="WP_077969475.1"
                     /transl_table=11
                     /translation="MRPDTPADHTAAAERLERTAEQYPEDAEQLLLQAAAYRELSGDRE
                     RATAYYDRLLAADATEHPILVRALKAANLWEYGHEAEARAIITGVRAAAPRDPAPWVIV
                     AEALETHDELQAAHETFSEGAALLVNRANDPAYETRPLLFGRHRVRRLLGAPHDDWDFL
                     ADHLHGSSVPLDELHDPKRVWSLGSENPAELRAEINRLQAELGSYRAALSRPFPVAVLH
                     WPAEELRELTTAFPALATEYPGTHADHLTRVEAALRELSASGTANLGTVTATVPSYEAF
                     AASEASPPEEASLLPQYATTLAARGRALPWPPAVTDACWCGSGRTYGECHGGG"
     gene            19256..19720
                     /locus_tag="GBW32_RS12960"
                     /old_locus_tag="GBW32_12950"
     CDS             19256..19720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893664.1"
                     /locus_tag="GBW32_RS12960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12950"
                     /product="very short patch repair endonuclease"
                     /protein_id="WP_077969474.1"
                     /transl_table=11
                     /translation="MKPVKPSSPGVSARMSRQMSRDTTPEVAVRKLLHASGHRYRLNMR
                     VRDMPRRTIDITFPRARVAVFLDGCFWHGCPIHATHPKANAEWWHQKLDRNMARDAETT
                     EHLTAVGWTVLRFWEHEVPVDVAERVADVVDRERREDGAPEIGRRGSASD"
     gene            complement(19807..20922)
                     /locus_tag="GBW32_RS12965"
                     /old_locus_tag="GBW32_12955"
     CDS             complement(19807..20922)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023524.5"
                     /locus_tag="GBW32_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12955"
                     /product="MrcB family domain-containing protein"
                     /protein_id="WP_077969473.1"
                     /transl_table=11
                     /translation="MGIRDMLGEIATTYDRHEGTKREVKGQQVLRAVERRKDLSLPSNL
                     TAKGHGGRTDAVLTPWVGLMDPTITSDPRQGVYLAYIFSADLVTVSLTLQQGVELLSER
                     LGRGVARLEHLRRDAARLRRAISRQRRQGWDDVLTLKSDAERPRAYEAASVIAKSYATA
                     DLPDESTLQEDLRHAVTLLHRTAPIHQVWWLGDGGGDELVVTYEGAEHIPSDDPLAGFQ
                     PKSSADYVATIAAKQMVKKRHHEALIRDFGHYVVDLGFDPIIRLMHPRDLVLRRRGADG
                     TYLKDHWLVEAKVVRKSNPTQAVREAVGQLREYSYFLYSERKKPVPHLLGLFSEEVGTY
                     SDYLEDQGIASIWRAERGWAGSPSAEKWGLV"
     misc_feature    21430..21445
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(21495..21508)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.62"
                     /tool="antismash"
     misc_feature    complement(21516..21530)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.67"
                     /tool="antismash"
     misc_feature    complement(21519..21533)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.19"
                     /tool="antismash"
     gene            21648..22493
                     /locus_tag="GBW32_RS12970"
                     /old_locus_tag="GBW32_12960"
     CDS             21648..22493
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382315.1"
                     /locus_tag="GBW32_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_12960"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077969472.1"
                     /transl_table=11
                     /translation="MAVGPTTRRRQLGSDLRRLRENAGLTLEEAGARVGVSKATLSRYE
                     TKEGTVKWPTVDALCREYDTTSEERAELVELAKGAKIQGWWRSLADPIPGSMNLMLTLE
                     DEVIREDHYACVYVPGLLQTRAYAVAVHRASEMRCSDREIQHSVDIRIRRQKLLQRPDP
                     PHIWAVLDEAVLRRRVGGDAVMREQMLHLRELGAGLHVTLQVLPFASGAHAAAVGNFLV
                     LGGPTEDLDVVYVDILGGGLFMEKPAELKRYKMTFEYLRAQALSIEASSAFIDRVCEEL
                     "
     misc_feature    22116..22118
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gcagcgactt gtagggccac aggtcactgc cgcagacgca ggccagcacg atccgtacga
       61 tcgcgtccgt cgggttctgg atcctcgggt cggcgacatc ctcgacgcgt atgtcatcgg
      121 ctgcgtacag cagggcggct cgcatgagcg aaactccttg atgggggggc gggcgttggg
      181 cgttgggcga tgggttgctc gtggtggacg acggggacgg gacgggtacc gaccgggccg
      241 ggtctcagcg cggttccagg cggagggtgg aggtgcgggc ccggtcggtc agcacatggt
      301 cgacgatggt cgtgtagtgc ccgtacttct cgcggtatgc ggcgtccacg cccgcgtact
      361 cccgcgggtc ggcctcattg aaggtcacgt cctggcgtac gccgccggcc tcgacatcgc
      421 cctgttcgcg ggtgcgcgtg ccccggtacc aaggcccgtc ctggcctttc accgaccgga
      481 cgtagacatg gtctccggcg cgtaccaccc acatggtcac agggttacgc agggtgccgt
      541 cgccgcgttc ggaccgcagc tcaagttcct ctgccgcgcc gatccgctca agttgtgagc
      601 cgttccaggt cttcatcggc tttccttccc ggtcaggagt ggtcgtgggg ggggtggtcg
      661 gaagcggggg tcgggaatgg gtattggatt attgggagga attggcggtc tgctggagaa
      721 gagccggagg agaagtcgga ggggaaaccg gaggccggcc ggttaccgga gggcggttac
      781 cggagggggg aaagtcggag tgaggtgggt caggagtcca aggtggcgtt gcccagcctg
      841 ctgaccatgg ccggatccct gtggtcgaag aacagtgact ccccggtgtc cagcttcgcg
      901 atggccgcca tctcgtcgga cctgaggtcg aagtcgaaga cgtcgaggtt ctccgccatg
      961 cgctcggggc gtaccgactt ggggatcacg acgacgttcc gctggatgag ccagcgcagc
     1021 acgacctgcg ccacggactt gtcgtgcgcg gaggcgaccg ccaccagtac ggggtcggtg
     1081 aagaggtggt tgcggccctc cgcgaagggg ccccacgact cgatctgcac cccacgctca
     1141 ctcatgagtt cctggtcggc ggcgcgctgg tggaaggggt gggtctcgac ctggttgacc
     1201 gcgggggtga tggcgttgtg gtcgatcagg tcaacgagcc ggtcgccgtg gaagttcgag
     1261 acgccgatgg ctcgggcgag gccgtcgcgg tgcaggctct ccatggcgcg ccaggagccg
     1321 tagacgtcgc cgaagggctg gtggatcaga tacaggtcga ggtagtcgag gccgagcttg
     1381 cgcagcgagg tgtcgaaccc gcgcctggcc tggtcctcac cggcgtcgga gacccagagc
     1441 ttggtggtga cgaagaggtc ctcgcgggcg atgccgctgc tcttgatcgc acggcccacg
     1501 gcctcttcat tgccgtacga ggcagctgtg tccaaggacc ggtaaccggc ggcgagggcg
     1561 tcggtcaccg cctgttcggc ctggtcgggc gggatctggt agacgccgaa gccgagtacg
     1621 ggcatctcga cgccgttgtt gagggtgacg gtccgcatgt ggccggatcc tctcttgagg
     1681 gggagggagt gagggggcgt gcggtcagcg ttcgagcatc tgccgggcgc cctcggggtg
     1741 ggtctcgccc gcggcaggcg gaaggctgct gagtctgtcg atctgctcgt cggtcagccg
     1801 gaggctttcg gcggcggtgt tctcctcgac ccgggccacg cgcttggtgc cggggatggg
     1861 ggcgatgtcg tcgcccttgg ccagcagcca ggcgatcgcg acctgtgccg gagtggcacc
     1921 cgcctgctcg gcgacggcct gcacctcgtc ggcgatacgc agattgcgct ggaagttctc
     1981 gccggagaag cgcgggttgc cgtaccggaa gtcgccttcc ttgaactcgt cggtggagcg
     2041 gatcgtgccg gtcaggaaac cgtggccgag cggggagaag ggcacgaggc cgatgttcag
     2101 ttcgcgcagc accggcagga cgcgctcctc aaggccgcgg gtccagagcg agtactccga
     2161 ctgaaccgcc gagaccggct ggacggcgtg agcccggcgg atggtgtcgg gtcccgcttc
     2221 cgagagaccg aacgcgcgga ccttcccctc accgatcagc tcgcccacgg cccccgcggt
     2281 ctcctcgatc ggggtgttcg ggtcgacgcg gtgctggtag tacaggtcga tgtggtcggt
     2341 gcccagccgc cgcagggatc cctcgacggc gctacggatg ttgtccgggc tcgaatccag
     2401 tgtccacgct ccggcgccgc cgtgcgagac gaggccgaac ttggtcgcga ggaccacctc
     2461 gtcgcggtgt cccttcagcg cgcggccgac gagctcctca ttgacgtacg ggccgtagat
     2521 ctcggcggtg tcgatgagtg tgacgcccag ttccagggcg cgatggaggg tacggacgga
     2581 ctccgcctcg tcggtcccgg agtcggtgta gccgtgggac atgcccatcg cgcccagccc
     2641 gatacgggaa acccgcaggt cgcccaggtt gatgtgctgc attactcgtc gcctctcgct
     2701 cttcgtcgta agccgtatgt cgcatgtcgt gcgtcgcgtg tcgtgcgtcg tgcgtcgtgc
     2761 gtcgtgcgtc gtgcgtcgca ttcgaatgcc gtacagcttg tgtcgcacgg cttgtgttgt
     2821 gcggccggtg tcctgcggcc ggtgtcgtgc gcctctgact gccgtcttcc agccgtctga
     2881 cggccgccgc agccggaacg taagtccgtg cccgtacgtc cgtgtccgta cgtccgtgcc
     2941 cgtacgtccg tgtccgtacg tccgtgcccg tacgtccgtg cccgtacgtc cccgcgacag
     3001 cgctacgcag gtgtcgcggc ggggcccacc tgctcaccgg gctggtggtg cacggtgtgc
     3061 ctgacgggct cggtgcgctc cctccagcgt gcggccgaac gcggggaaca gccaggccga
     3121 ggtcttcggg ggagcggcca gccggggtgt gacagggccc ccctcgtagg cgcgtcacgg
     3181 tggcggcgga gagacacttg gaccatggca ccggaacaga gcagcggcga cgaactgggg
     3241 cgtttcctgc gcgcccgccg tacccagacc agcccccggt ccgcaggcct gacgcctggc
     3301 cccggcgtac gccgcacacc cggactgcgc cgtgaggagc tggccacact cgccggggtc
     3361 agcatcgact actacacccg catggagcgc ggcaaggaga ccaggccgag ccccgccgtg
     3421 gtcgacgccc tcgcccgcgc cctgcgactc gacgacgccg aacaccagca cctgcgcgac
     3481 ctggccgtcc gcgccgcccg ctacgcgcac cccgccccgg agccgagccg tacggtccgt
     3541 ccccacctgg agctgctgct tgagaccgta cggcccaacc cggcctacgt cgtcagccgc
     3601 agcatggacc tgctcgccca caaccccggt ggcctcgccc tgtacgcggg catcgccgac
     3661 tggccggcca gacagcgcaa cctcgcccgt tacctcttcc tgcaccccgc cgcccgcgag
     3721 gtcttcggcg actgggagaa ccagatccgc ggctgcgtcg cccggctgcg ggccctggcc
     3781 ggcatcgacc ccgacgcgcc cgacctcacc cggctcgttg gcgaactcct gctcaagagc
     3841 cccgacttcg cgggcctttg ggagcgctac gaggttacgg gccgcaagta caccaccaag
     3901 accttccacc atccccgggt cggcaggctc accgtcggct tccagggcat ggacctcgaa
     3961 ggcaccccgg gacagcgcat ggtggtctat acggcagaac ctggcacgcc ggaccacgac
     4021 gccatggttc ttctcgacat gaccgcacct gagcagcaga cgagctcggc atcggcccga
     4081 tcggcggcct cgaccgaacc ggtcgcctcg accaagccga caggtccagc agacgcgaca
     4141 ggttcaacag gtgcgacagg gccaacaggg ccgacaggtc cgacaggtcc gatatccgag
     4201 caggcccggg aacgcggacg acgggactga gccgacacct cctcggggcc cgctccgctc
     4261 actcctgacg tgcagacgtc ggacaccgga tgtcgggcac cggacaccgg acaccggcgc
     4321 gggactggac cggaccggcc accggccccc gccgggcgcc tacggctcgg cgtacccgtc
     4381 cggcgcggct cggcgcaccc gtcaggcacc cgctcggcgc acccgtcaga cgcggctcgg
     4441 cgcacccctc aggcaccgct cggcgcaccc gtcaggccag ccccgtcgcc cccgccgtgt
     4501 ccgccctgcc gaacaccggg gcgagtacga gttgcgccgc cccgtccgcg attcccctga
     4561 cgccgccggg ggcgagccgg accggcacgg cgggccccgc tccctcgcgt cccgctcgcc
     4621 gcgcgaggac ggcctcgacc ccggcgacgt accgctcgct ctccgccgcg atggtcctgc
     4681 cgccgagcag gacgaggtcg atgtcgagca gcccgacgag gttcgacgcc gcggtgccca
     4741 gtacccgtgc ggcctcgtcg ggatccccgc gtccgacggc ggccaggcac agcgcctcga
     4801 cgcagccacg gtcaccgcac ccgcagagcg gcccgtccaa ctggatcacc tggtggccga
     4861 attcgcccgc gccggtccgt gcaccccggt gcaggaggcc accgagtacg agcccggcgc
     4921 cgagccccgt accgaggtgc agataggcga acgaccctcc tcctgggccg agtcgcagcc
     4981 ccgaccctga gcttgatccc gatccgggcc gcactccctc tcccggcccc gatccgggtc
     5041 gcactccctc tcccggcccc gatccgggcc gcgtcccctc ccccgccccg aataccggcc
     5101 ccgcgatgtc cgtcatcccg agagccgggc cgagtgccgc cgcgttggtg tccttgtcca
     5161 cggtcacggg gaggccgagc cgcgcggcga gggcgtcccg cagtgggaac ccgtccaggt
     5221 cggggaagcc ggtgacccgg tgcagcacac cctcgcggtg gtccaggggc ccgggaacgc
     5281 cgaccccgac acccagcacc cggccgcgcg gccccggggt gagccgaccc ggctgacccg
     5341 gccgaccggc tccgtcatcg gcggaacccc cgccgtcaac ggatccccgg tcaccgggga
     5401 ccgcccccaa gagcaattcc acctcccccg ccgtcgcctc gatgacggcg tccgcgcccg
     5461 cgccgaggtc gagtggggcc gtccgttcgc gcacggtcgt gcccgcgagg tcgacgagta
     5521 ccgtcctgag ttcatcgcgg tcgaggtgga caccgaccgc gtacgcggcc gtcggaacca
     5581 gccgcagcac cgtcctgggc ttcccccctg tggaggcgcg gcgccccgcc tcggtggcga
     5641 ggcccgaggt gcgcagccga gcggtgatct tgctgacggc ctgcggggtg agcccggtgc
     5701 gttcggccag ttcgagtctg ctgatcccgg tctcaccgga gacgcggagg aggtcgagca
     5761 ccagggcggt gttgtgcccg cgcagcacgg gcaggttggc cccgccacct ttggggtgcg
     5821 gactcttcgg gtgcggacag ttcgggtgcg gactcttcgg gtgggggcgg ttcgggtggg
     5881 gacggttcgg gcgtggaccg tcggggagcg gactgttggg gggcggactg ttggggagtg
     5941 atcccccggt tcgcccggct gccgccgccc gccctgctga cgctgacgct gacgctgacg
     6001 ctgacggccc tgacgctgcc ggccccgccc cggtcggccc actcggccca cccggctcac
     6061 ccccgccgcc acacccgctc gggccaccgg acccacccgg gccgcccacc tcacccgctc
     6121 cgccgggccc gcccgtccca ctcgtgtcgt tcaccatcac acgcccattg tcccgtgcgc
     6181 ttgcactttg gcaacaccgt tgcttaagtg ggtcgtatga ctggtttgac tggtaccgat
     6241 cccgaacccg aggccggctc cggctcgcgc cccccgctcc gggtcgccct catcggctac
     6301 ggcctcgcgg gctccgtctt ccacgcgccg ctcatcgccg ccacggacgg gctggtgctc
     6361 gacaccatcg tcacgtccaa tccggagcgg cgcgagcagg cgctcgccga attccccgac
     6421 gtacggttcg ccggctcccc cgacgagctg tgggccgacg cggcccgcat cgacctcgtg
     6481 gtcgtcgcca cgcccaacaa gacgcacgtg gccctggcca aggcggcgct cggcgcgggg
     6541 ctgcccgtcg tcgtcgacaa gccggtcgcg ggtaccgcca ccgaggcccg tgagctggac
     6601 gccctggccc gcgagcgcgg tctgctgctc tccgtcttcc agaaccgccg ctgggacaac
     6661 gacttccgca cgctgcacaa gctgctcgcc cacggtgatc tgggtgacgt gtaccgcttc
     6721 gagtcccgtt tcgaacgctg gcgcccgcac cccaagggcg gctggcgcga gtcgggcgac
     6781 ccggcggaga tcggcggcct gctgtacgac ctcggcagcc atgtcgtgga ccaggcgctg
     6841 acactcttcg gcccggccgc ctccgtatac gcggagtccg acgtccgtcg cgcgggcgcg
     6901 gaggccgacg acgacacgtt catcgcgatc acccacgaga gcggcgtccg ttcccacctc
     6961 tacgtcagcg ccaccacggc gcagctcggc ccgcgcttca gggtgctcgg ctcgacggcc
     7021 ggctatgtga agtacggcct ggacccgcag gaggcggcgc tgcgcgaggg caggcgcccg
     7081 gcgaagggtg gggcgtgggg cgaggagtcc cctgagctgt ggggccggct cggcgcgggt
     7141 gagtcacccc tctccggcgg gggcgagccg gtacgtacgc tccccggcga ctacccggcg
     7201 tactacgccg ccgtcgccgc gaccctgcgc ggcgacgggc ccagcccggt gaccgcgctg
     7261 gaggcggcgg ccacgctcga cgtactcgaa gcggcgcgcc gctcggccga cgagggcgtg
     7321 accgtgcccc tcggcgcgga gcccccggcc cgcgcggaca cacgtatccc cacgaagtcc
     7381 cccactatcg aggagacgac gacatgagcg gttccagcgg cggtacgagc ggtacgaagg
     7441 gcgcgggcgg ccccgatgtc cccgagctgg aggcccagga gaagcggctg gtgctgaagc
     7501 gtttcacgta cgagaacgcg tgggcgctcg gcagcctgct ggtggagctg gcccgtgagc
     7561 ggcgggcccc cgtggccatc gacatccggc gcggcgcgca gcgggtgttc cacgcggcgc
     7621 tgcccggttc gagcgcggac aacgacgcgt ggatcgaacg caagcgccgc gtcgtggagc
     7681 ggtacggcga gagctcgtac ctcgtcggca gccgtttccg cgcgaagggc acgacgttcg
     7741 aggactcctc ccgcctcgac caggaccgtt acgcggcgca cggcggcgcg ttcccgatcg
     7801 ccgtcgaggg tgcgggtgtg gtcggcgtgg tcgtggtctc ggggctgccg cagatcgagg
     7861 accacgcact cgtcgtagcg gcgcttgaac ggttcaccgc gcagcggtcc gtctccgacg
     7921 cgttcgtctc gggcagttcg ggcgacgggg cctgagccga gccgcgtcag gcgccccgac
     7981 agcgccccgg ccgacagtcg gcctccgccc cgtgcacacc tggggcggag gccgacggct
     8041 gtgaggctgt tgtccttccg cgtctgactg ctgcgttctt gctggtcacg ggtagggatg
     8101 gcgtccgtgc gcgagagatg gggcgtcgtt acgtcacgcc cgtggaggtc tcggacgccg
     8161 tcggttgtgg gaccgctaaa acagcgcgag cttgccgccg tcgaagccgc ggctgtcctc
     8221 gccgacgacc gcgtcctcct tgacggaccg cgaggcgatc acgacggccg tcggctccgc
     8281 atcccgcccc gccgtctcgt cgacccggcc gcgcagccgg tctgggaaca ctctgaccag
     8341 ggcacgctca cgccagcggc ggaagaacgc acatacccgg tcccgcggtg gcaagtccga
     8401 ggcggggtcg gccacttggt cccgttgtcc accggataac gcgcagcgtc cagcatcgcc
     8461 cggtggcagt acacctccgg cgccccgccc ccgcatccag cccggcaccg gcagcggcac
     8521 cggcggcagc ggcagcggca gcggcagcgg ccggaccacg acccactccg cgtccgtcat
     8581 gtccgaggga taccgccgaa cacactccgg atgaccggcc gcgctccaga accggtgagc
     8641 gacacaaaca cacgacaccg caaccgagtt gaactcgacc tgcgcgagca cgtacccgtg
     8701 gggggaggaa gaactcgcga aggacgtgta ctgcttcgcc cacaggtcag ccactgattc
     8761 ccggtcttgc gctgagcggg aaacaggaac accctgggat gggccaccga tgtcgggaag
     8821 gctgctcgcg ggcaacaccg cacgccactc tccggtgggc cgcaccgtcc gcacccgtgc
     8881 catatctgcc ggttcccatg cctgatcacc cggcgttcag cagctgttgc ccccgcatcc
     8941 gacatcccgc cccagcggag gaacacgcca tgagcgcgtg cgatcagccc ttgcccgaaa
     9001 aatccccaga tcagcgtccg gcgaccggcg acacgctgtc cggcgcgtca gccgcaccgg
     9061 atgagtgcca cgacgtacgg gctcacttcg acgccgtctt cactcaactg ctgcccgctc
     9121 tgtatcggcg ctcacgctcc ctttccgctg cccgcgacct ggccgaggac gcggtacacg
     9181 acacctatct caaactgtcg gcacgtccac ggtcactcct tggccactcc catccctatg
     9241 cctacgcgtg cagggctctg ctgagcgtga cgagggacgc ttggcggcgt gagcggcgga
     9301 tggtgccgac cggtgaactg gaggccgggc cgaccgggtt cgaggaaggc ccggacgccc
     9361 ggctcgcaga gctgatggcc ctgcgtctcc tgcggtccct gaccgagcgg caggcgcagg
     9421 ccgtgatcct cgtcgacatc gagggccaca cactggacga ggcggcgcgc cggctcggtc
     9481 tgcaccgagg gaccgttgcg cagctgcgtc ggagggcgct ggagagcctg cgggacaaga
     9541 cagaggccga gtcgaaggac ttgggccccg cacaccgccc gcgcgggtag gggggcagcg
     9601 agttcgccgc cccccttccc cagggtcgcc cgccctcccg gcagggccgc cgatttcacc
     9661 cgaacgggtg tcatacgaag accgcccctc cttcgtgttc tccacaacga gccgggacgg
     9721 ccgccggtca tctcaggccg gcgccgtgca cgccgatggt gcgctccacc ccgagctcgc
     9781 agcaagcgcc cgtccgggca caacctgtga ggagaaaacc atggaaaaca ccgacgtcta
     9841 cgaacccccg gtcctcgtcg aggccggcgg gttcatggag ctgaccctgg gcgcggacaa
     9901 gggcggcgtc accgacttcg ccggatggtt cgacatcttc agcgacttcc agggctgaga
     9961 cctcggcccg ccccggcaac cgtccggggc gggccgcacc atgaagggac tgagcatgat
    10021 gcggggttca tggttcgccg tgctgcccga ccgcgaggcc gccgaggagg ccgccgcccg
    10081 gctgcgggcc tgggccgggc aggaagtgcg gcacgcctca gggcggccgt ggctgctggg
    10141 ctgctgggag ccgtccgaag tgaccaccgc gcgggccggt ggccacgcgg ccgcggttct
    10201 cggcaggcac gtcctgacgg aagcggagct ggccgcccgc ttgaacaccg tggtcaccac
    10261 gggcgacgtg gaacacttcg cggacgaatg ggctgggagc tttcacctca tcctgtccgt
    10321 gcaccggcag atccgggtac gaggcaccgc gtccgccacg cgccgagtat tcaccgtgcg
    10381 tcagaaaggc gccacgatcg cgtccgaccg cgccgacgta ctggccgcac tctcaggggc
    10441 ggccgtcgac gtgcgtaggg tggcgatgcg gctgctgagc cctcaagtgg cccatccctt
    10501 cggagaacac tccctctgga cgggagtcga ctacgtcccc gcggaccagg aactcgtcgt
    10561 cgagccgggc ggccgaacac acctcgcacg ttggtggtcg ccccccgaac cggtgcttcc
    10621 gatggcagag ggcgccgaac gggtccgtgc ggcgcttcga gcgtcgctga acacctgcaa
    10681 gggtgccgcc tccgtcctga gctgcgacct ttccggtggc atggactcca ccagtctgtg
    10741 cttcctggca gtcgggaagc cctcgcgtct ggtcaccgtc cgacgggagg cggcggaccc
    10801 cggcaacgac gacgccatgt gggccgaggc agccgcaggc cacctgtccg acggtcacca
    10861 cctcacgctg cgggacggcg aaatccccgc ctggtacgcc gatgtcgcta cccccgccga
    10921 cgacagggag gagccgtaca aggacatccg cggtcgggcc taccggcgcg cgatggcccg
    10981 gcacatggcc aacctgggag cacagaccca tgtgtgcggg gtcggcggcg acgaactgtt
    11041 cggttccagt cccgcctacc tgcacgacct gctacgcctg cgtcctctcc tgactctggg
    11101 acgcgtggcc gcccaccgtg ccacagcgcg ctggccgctc acgacggcgc ttcgcgcact
    11161 gtcgcagcgg ggcaccttcg atgactgggt caagggtgcg gccgacggtt tgagtgccgc
    11221 tccacgacgc gtgcagctgg actggatgcc gcggctgcga ctgcccccct gggcgacgcc
    11281 cgaggcggcg gagctggtgc ggggcgtcct gcacgaggcg gctcggtgtt cctgggagcc
    11341 gctctccccc tcgcgcgtac agcaccagac ggcccagcat gtgcgtacgg caggcatgag
    11401 cgtacgccag atcgaccggc tgatgaggtc caccggggcc ggcatggctg cccctttcct
    11461 ggacgaccag gtgatcaccg ccgccatgtc ggtacgtctt gaggaccgct tctccgcgac
    11521 cagcttcaag cccctgctcg cccaggcggt agctgcccat atgccgtcgg cgctgctgga
    11581 gcgcaggacg aagggcgact tcagtttcga cctgcaccag gcgatgcacc gcagaagagc
    11641 cgatctgctc gaactcaccg acaccctccg gctgggcgaa ctcggcctca tcgacgcggc
    11701 ggcactgcgc caagccgtgc tcacacccca cccgcggggc atgacgctga ccccgctgga
    11761 agcgacgttc tcctgcgagt cgtggctgcg cggcctcgac agccgatccc tccacctggc
    11821 acacccgaaa ggacacacct gatgcacctc gcccttcgct ccgacgtctc cgcgacggac
    11881 ggcgacttcg acatggtgct gctgaacgag tgcaccggcc gctactggta tctgaacgcg
    11941 acaggcgcct tcatcctgcg gctcttcctg gacggttcgg aacgggccga ggtcgcacgg
    12001 agggcggccg agcggttccc ggtgagcgag gagaaggcat cccaggacat cgagacgctt
    12061 gtgtcgtccc tgtgcaccgc cgacctcgtc aaggtgaagg catgagcacg cacatggttc
    12121 cggagagcgg aatccgcctg cccatgcgga agcaggtccc cgcgcggaca gcggtggccg
    12181 cggccaggct gctcgccctg ctgcccccac gccatatccg gcgcgctctg aacctgttgc
    12241 ggcgcggtgc ccgtcccgcc ggtttccagg acgccctgac cgcgcgcacg gccgtgatct
    12301 ccgtcagcac ccgctgtgcg gggggcgaag gctgcctaca gcgttccctg gccaccgcca
    12361 tcctgtgccg catggcggga cactggccgc agtggtgcgc cggagtccgc acgcagccac
    12421 tgcgcgccca cgcctgggtc gaggtcgagg gcgttcccgt gggagagccc tatcccgcgc
    12481 actacttcca actgctggta cacgtgcccg aagtgccgtc cgaacgcggc tgagggacga
    12541 ggcgaccgca gccccgaccc acaccaaggg gtgatcgggc agcgcaccgc ccacgacgcg
    12601 ctcggtcccc ggtggaaagt cggaggccgg cggctaagcg tccttgaact cctgccgctg
    12661 gcgcccgagc ccctcgacct ccagctccac gacatccccc gcacgcaggt agggcttggg
    12721 ctccggctgg cccatggcca ctcccgccgg tgtaccggtg ttgatgatgt cccccgggta
    12781 gagggtcatg aactggctta cgtagcggac cacttcgccg acggggaaga tctggtcggc
    12841 ggtggtgccg ttctgccgca gctcaccgtt gacccacagg cgcagggcaa gcgcctgcgg
    12901 gtcgggcacc tcgtccgcgg tgaccagcca ggggccgagc gggttgaacg tctcgcagtt
    12961 cttgcccttg tcccacgtac cgccgcgctc gatctggaac tcgcgctccg agacgtcgtt
    13021 ggcgacggcg tatcccgcga catgagcgag cccgtcctcc gatgtatcga ggtagcgggc
    13081 ggtctttccg atgacgacgg ccaactccac ctcccagtcc gtcctggtcg agccgcgcgg
    13141 cacgagcacc gtgtcgtcgg ggcccacgac ggtgtcggcc gccttgaaga agatgatcgg
    13201 ctcgggcggt gcgggcgcac ccgtctcggc ggcgtggtcg tggtagttga gcccgatgca
    13261 cacgaccttc cccacgccgg ccagaggtgc cccgtagcgc agcccaactg tgtccacctc
    13321 gggcaggtca ccagcgcgct cggcctcccg tacgcgggcg agggccccgg tggtgtcggc
    13381 gagaagcgcc ccgtcgatgt cgccgatcag cccggaaaga tcccggagca cgccctcggc
    13441 gtcgagcagc gcgggccgtt ccgaccctgc cgtaccgact cgcagcaact tcatgatcac
    13501 cctccagata gacagcacgc acggccacac agcgcacgcg gcgcacaccg tgcgcacggc
    13561 ggccaccatg cacacggcgg tcaccgcgca cacggcggtc agcaccgcat ggccggcgaa
    13621 aagcatcagc catcggatct ttggcagatc ctccaaatgg cgtcgagggc cgatactcca
    13681 gggtcggggt ccgctccgca ataccccgtt cacggagcgg accacctcac ccacgaggct
    13741 tcgcacctca cgcctttcgc gtaccgtcgg ccgcggcaag cacggcggag gcgacgctgc
    13801 gggcctgccc tgcggcgtcc gctcgggtac ggcttgtaga gccgtggcac ggcgggggcc
    13861 gtcttccacg acggactcgg cccactcgct tgtccgtacg ccgctgggga gcgccgagac
    13921 cacatcaacg acagcactca gttgcccata ggggcggttc aggcggccgg ggagagtgcg
    13981 ggtgcgggcg tagcggccgg catgtcccgg tagagcaccg cacgctcgac ggcgctccac
    14041 gcggtactgg tgaccaggta gagcgcggcc gcgagcggca cgacggcgac gctgaagagc
    14101 gtcatgaacg acatcagcgg catcagcttg gtgatggcgc cgatcccggg cacctcggtc
    14161 ccgggcgcct ccgtcccgcc cgccttgtcg gtccgcgtgc tgccggagac ctgagcgagc
    14221 ggggaggccg cggaggccat ctgccgcttg gtcctgcggt agttgaaggt ggcgaccacg
    14281 atgacgaccg cgaagatccc cacgtagacg agaccgctgt caccgaaggg cccgccgttg
    14341 ccgagggcgt ccttccagcg gtcgccgagt ggcgcggcga agagggtgtg accgaggagg
    14401 gagttggggt cgccgccgat gctggagctg gagaacaggt ggtacagcag catgaaggcg
    14461 gggatctgga acaggctggg caggcagccg gagaggggcg agaccttctc ggccgcgtgc
    14521 agttccagca cggcttgtcg tagcttctcc gggttcttga cgtgcttctt ccgcaactcg
    14581 gcgatctgcg gctgaagctt ggcgcgggcc cgctggccgc gcgcggaggc ccgggagagc
    14641 gggtggacga gcagccgtac gcaggtggtg aaaaggatga tggcgacggc ggtggacgcg
    14701 tcgtggaaga gcgggtggag caggtcggcg aagtgcgcga ccagactggc gaacgcggag
    14761 aagaaagcgg acatacggga ccctccgaag tgactcgtac tgctcgtggt gctcgtggtg
    14821 ctcgtcgtgc ctgactcgcg gcccggcccg cacccggcga ggggcgcggc ggccgttggc
    14881 ggcgtacgga cagctcacgt acgcaaggca tgcgcatggg gcgtgcacag ggacgcgcca
    14941 cacggagatg cgcacgggga cacgtacgga gatgcgtacg gggatacgca gggagatgcg
    15001 tcggcatgac ggcccgcgag gagtctcgcg cgactggagg gcggagggcg gtacgtgact
    15061 gacgccagta cgagaacgag ggctcagccc aggagagaaa gagaagagat ggggtgagcc
    15121 gagccgtcag gctttcaggc gtcaggcatc aggcatcagg tcaggtaatc gaccggagcc
    15181 aagtccgtat cgcgaacgcg gttaccgctg tatcgcgatg acctacgcgg ccgtcaggag
    15241 acggcggccg ggtgcgcggg gcctggggcg gcccgaggcg tcggggtctc gctggggcag
    15301 gaacgcggtg cggtgctcac ggtccctgat ggccgtacgt acccgggtgg gcggtacggc
    15361 gggggcgcag cgcgcggcga tgacggagca ggcgacgagc gcggagcccg cggccgcggt
    15421 ggccgtggcg gcgagcgcga cggcaccgga gagaccgccg ccgtcggtca gcagaacgcc
    15481 ggcgagcagg aagaggacga gcccgacgga gcgtgaggtg gtccacctgc cggccatctc
    15541 caccccctgg tcggatcggt tcacctgtac tcgttcaccc gttcacccgt ttatacagga
    15601 tcggtgacga cgggctgaag gagtcggcgg ggtgcgagca gtgcccggcc gaccggatcc
    15661 gaggggtcgg ggtcgagtcc gggcccgggg atcatctcca catccggcac aggcacgggt
    15721 acggcgcaga cggggcactc cccgtacgag ccgagacgcg taccgcaggc gaagtggcgg
    15781 aactcacgcg gcggcagcga gtcgccgacg tggtcgcgcc cccaggcgcc gagcgcgcgg
    15841 atgacgggcc agagggcgat accggcgtcg gtcagtacgt actcgtcgcg gggcggcgac
    15901 tcctggtagc gccgcttgtc gagtacgccg gcctcggtga gggagtgcag ccgggcggcg
    15961 aggacggcgc gcggaatgcc caggtgaacg aggaagtcgt tgtagcggcg gacgccgtag
    16021 agggcgtcgc ggacgacgag gagggtccac cgctctccga cgatctccag cgtccgcgcg
    16081 atggagcact gctgggccgt gtagtccttg ccgagagcca tgcccccact gtagccacca
    16141 ctcagcggcc ggttcaatga atgaactcac ggtgctacgg tagccgcgac caagtaggtt
    16201 cattcatcga actcacctaa cccctggtga agggctggag ctggaccatg acgggtacgg
    16261 gtgcgggtac ggacgcgggt acgggtacgg gtacggctgt gggcacagcc gcgagagtga
    16321 gagcgggcac ggcggcggac gcgggtgcgg gcgccggggg gaccgcgggc gcgagccgga
    16381 caactgtggc cctgaccctg atcagcgcgg taacagcgat agccctgatg aactacacca
    16441 ccccgatgtt gacgctcccc ggcatgatcg cgtcattcgg aacgccgctg tccgcccagg
    16501 catggctgct gaacggaacg ccgctcggcc tggccgcgct gctcctggtc gcgggcagcc
    16561 tcgccgacga ctacggccgc cgcagactct tcctgaccgg cacgctggcg ctcggggtga
    16621 ccacgctgct cggctcgctc gcgacgacga cgttgctgtt cacgctggcc agggccgcgc
    16681 aggggatggc gagcgcggcg atcatcacga gcggcctcgg cctgatcgtg cacgcgttcc
    16741 cgccgggcca cacgaggatc cgggcgacgg ggatctgggg cgcgtgcgtc agcggcggta
    16801 tcgcactcgg cccgctgctc gcgggggtga cggcggggct tggctggcgg gcggtgtacg
    16861 tcgtactcgg cgcggcggcc ctgctcgccg cggcgttcgt accgcgcgta ctgaaggagt
    16921 cgcgcgcacc gaggccgggc cgcccggacc tgctgggcgc gctgacgctg gggcttgcgt
    16981 cggtgtcgct gttggccgcc ctgacgctgg gcagggacgg ctggctgcgg gcgccggtgt
    17041 ggggtctgct ggcggcgacg gtggtactgg cgggggcgtt cgtcctggtg gagcgccgag
    17101 gcgaggcacc gatgctggac ctcgggctgc tgcgccaccg cggtttcctg gcggccacgt
    17161 cgggggcgct gttcacgggg gtggcggtga tcggcctgtt cagctacctg gcggcgctga
    17221 tccagagcac gctgggtctg ggcacgctcg gggtggcgtg gctgttcgtg ctctgggcgg
    17281 gcacggggtt cgtggtggcg ctccaggcga ggcggctggc gggcagggtg ggggcccgcc
    17341 accagctggc actcggcttc gtactgtccg ccgcgggggt actgacgatg ctgggcgccc
    17401 tgaacgccgg ctcgtggcca cggctggtgc cgggcctgct gatcgcgggg gtgggcaacg
    17461 ggttgctgaa cgcggcgctg ccgcagctgt cggtggagag cgtggagccg caacgggcgg
    17521 cgatgggctc gggagccaac aacacggccc gctacatcgg ttcatcggcg ggagtggccc
    17581 tctccatagc ggtggcgacc tcggcgacgg gcgaaacccc ggcgaaggcg ctggcacacg
    17641 gagcggacca cgcgctactg gtctcggcgg ccttggcact gctgggggca ctcgtcaccc
    17701 tcgcactacg ggacaggagg gacccggtcg cgaggaggtg aggagcggcg cgaaggaaag
    17761 atcgaggagg cggcccgaga acggagcacg ccggaaacac ggaagaccgg gcgggggccg
    17821 aggcaggagc agcgctgggc ggagcccaag agcgggaacg gcaaggggag ccgcggccac
    17881 acgatcacga tcacggcccc gaccccggcc ccgaccccgg cccgagagga ccagacgtgc
    17941 ggatcggata cggaccggat gctgaggggc tgcggagggg atacggaggg gatacggagg
    18001 ggtgtgacga ccgccccctc ttacccgacg ccgaacggca gtacggtgtc ccgtatgcga
    18061 ccggacacgc ctgccgacca caccgctgcc gccgagcgcc ttgagcgcac ggccgaacag
    18121 tatccggagg acgccgagca actgctgctc caggcggcgg cctaccgaga actgtccggc
    18181 gaccgcgagc gggccacggc ctactacgac cgcctgctgg ccgcggacgc cacggagcac
    18241 ccgatactgg tccgcgccct gaaggcggcc aacctctggg agtacggcca cgaggcggag
    18301 gcacgcgcga tcatcacggg cgtacgggcc gcggccccca gggacccggc gccgtgggtg
    18361 atcgtggcgg aagccctgga gacgcacgac gaactccagg cggcccacga aacgttctcg
    18421 gagggcgcgg cgctcctggt gaaccgcgcg aacgacccgg cgtacgaaac acgccccctc
    18481 ctcttcggcc gccaccgcgt acgccgcctg ctgggcgccc cgcacgacga ctgggacttc
    18541 ctggcggacc acctgcacgg ctcgtcggtc cccctggacg aactgcacga cccgaaacgg
    18601 gtatggtccc tgggctcgga gaacccggcg gagctgcgcg cggagatcaa ccgcctgcaa
    18661 gcggaactgg gctcgtaccg agcggcgctg tcgcgcccgt tcccggtcgc ggtactgcac
    18721 tggccggccg aggaactccg cgaactgacc acagcgttcc cggcgttggc gaccgagtac
    18781 ccgggcacac acgcggacca cctgacccgg gtggaggcgg ccctacgcga actctcggcc
    18841 tcgggcacgg cgaacttggg cacggtaacg gccacggtcc cgtcgtacga ggcattcgcg
    18901 gcgtcggagg cgtcgccccc ggaggaggcc tcactactcc cgcagtacgc gacgacgctg
    18961 gccgcgcggg ggcgtgcgct gccttggccg ccggcggtca cggatgcttg ttggtgcggg
    19021 tcggggagga cttatgggga gtgtcatggg ggtgggtgag gcagcgcgaa cgctgaccgc
    19081 accccaccga tgaccggacg accgtatggc cggatgacca ctcgacggag ccctgaccgg
    19141 aatccctctt gtccgttctt cacgtaaccg tcacacttgg cccttcagac tgaccaggac
    19201 gccgggccgc tcgtacgtac gcgcacaggt cctgcctgta cgcgcgagga agcagatgaa
    19261 gccggtcaag ccgtcctccc ctggtgtctc cgcgcgcatg agtcgccaga tgagccggga
    19321 caccacccct gaggtcgccg tgcgcaaact gctgcacgcc tctgggcacc gttaccgcct
    19381 caacatgcgc gtacgcgaca tgcctcgccg aaccatcgac atcacgttcc cccgtgcgcg
    19441 cgtcgccgtg tttctcgacg gctgtttctg gcacggttgt ccgatccacg cgacgcaccc
    19501 caaggcgaac gccgagtggt ggcaccagaa gctcgaccgc aacatggcgc gggacgcgga
    19561 gacaaccgag cacctgacag cggtgggctg gacggtcctg cggttctggg aacacgaagt
    19621 acccgtcgat gtcgccgaac gggtggcgga cgtcgtcgat cgggagcggc gggaagatgg
    19681 agcaccggaa attggccgtc gaggttcagc gtcagattga ggagcatccc aagatgggag
    19741 gcaaccgtcg agaacaatcg ccacagaccg gctgggcgca gcaccaaacc cgccatgctc
    19801 tgccttctaa accaaccccc acttttctgc gctcggtgac ccagcccaac ccctctctgc
    19861 tcgccaaata gacgctattc cctgatcctc caagtagtcc gagtaagtac ctacttcctc
    19921 ggagaagaga ccgagtaggt gcgggacagg cttctttctt tcgctgtaca gaaagtagct
    19981 gtactcccgg agttgcccca cagcttcacg tacggcttgc gtgggattcg atttgcgcac
    20041 caccttggct tccaccagcc agtgatcctt caggtacgtc ccatccgccc ccctgcgtcg
    20101 cagcaccaag tccctcggat gcatcagtct gatgatcggg tcgaacccaa ggtcgacgac
    20161 gtagtgccca aagtccctga tgagagcctc atgatggcgc tttttcacca tctgtttggc
    20221 ggctattgtg gcgacataat ccgcactgct cttcggctga aacccagcca gcggatcgtc
    20281 gctcgggata tgttctgcgc cctcgtacgt cactaccaac tcatcacctc ctccgtcgcc
    20341 aagccaccac acttggtgga tcggggctgt ccgatggagg agtgttacag cgtgcctcag
    20401 gtcttcctgc aaagtggatt catcgggcag atcagcagtt gcgtagctct tcgctataac
    20461 gctggccgct tcatacgccc tcggccgctc ggcatcgctt ttgagtgtca gaacatcatc
    20521 ccaaccctgt cgtcgctgcc gggagatagc gcgccgcaga cgtgccgcat cccttcggag
    20581 atgctccaga cgcgcaaccc ccctcccaag acgttcactg agaagttcca caccttgttg
    20641 cagcgtcaac gagacagtga ccaaatccgc gctgaagata taggccaaat aaaccccctg
    20701 cctcgggtcg ctggtgatcg tggggtccat caaccccacc caaggcgtca ataccgcgtc
    20761 ggtgcgccca ccgtggcctt tggccgtcaa gttcgagggg agcgacaggt ccttccgcct
    20821 ctcaacggcg cgcaggacct gctgcccctt cacctcccgc ttcgtaccct cgtgccggtc
    20881 gtacgtagta gcgatctcgc ccaacatgtc tctgatcccc atggggcgaa cctatcgcca
    20941 caggagcatc gtcaaccagg cattatgcga gccgcacctc ccgccgcccc acgacggtag
    21001 gacgaactcg ccttctcaca ggagaaggtt ccgcctcctc gtggccacgg atctcgcacc
    21061 acgtgacttt gtggtccgct cgaagaatga cgccccagcg ggtcgccagc gcctccagga
    21121 ggacgagtcc gcgtccggat tccgccccgg cctcggccgc gagaagggtc gggagggctc
    21181 gggcgtcagg atcacgaacc tcaatcctca agcacccctc actgaaagac atggccaagg
    21241 ttccaggact ccccacccct acatggatca tcacgttggt gaccagttcg ctcgcacaca
    21301 cctccgcgga gtcggaaagt ccaggctgac cccagatcgt cacttggtct ctcaccagcc
    21361 gccgtagaac aggtagaacc gacggctcgg ccaggaaggg ctcctcccga gcctctttcc
    21421 acgccagact caacgactgg ttcacgcgga ccgcctccaa ctcaaacctg cgcgcacttg
    21481 ttgtgctcga cgcgacacac agagtgacag tgaaactctc aaaatgaaac tctcactgga
    21541 gataagtagg agcgaggccc gtcatcgcgc gcccccagcg cgcgccttct tgcccaaacg
    21601 gccacccacc aggaaggatc aaccgatcga tgcgaagggt cagtgttatg gcagtaggcc
    21661 ccaccacccg tcggcgccaa ctgggttccg atctgcgccg cttgcgggag aacgcaggac
    21721 tcactctcga agaggccgga gctcgtgtcg gggtgtccaa ggccactctc agtcgatacg
    21781 agaccaaaga aggcaccgtg aagtggccca cggtggacgc cctgtgtcgc gagtacgaca
    21841 cgacttccga agaacgagca gagctcgttg agttggccaa aggcgcgaaa atccaaggct
    21901 ggtggcgttc actcgcggac cccatcccag ggtcgatgaa ccttatgctc accctggagg
    21961 acgaggtcat ccgcgaagac cactacgcct gcgtgtacgt tccagggttg ctacagaccc
    22021 gtgcatacgc cgtagccgta caccgtgctt cagagatgcg ctgttccgat cgcgagattc
    22081 agcactcggt ggatatccgc ataaggcgcc aaaagttact tcagcgtcct gatcccccgc
    22141 acatctgggc cgtcctcgac gaagcggtac tccgccgtcg tgtcggggga gacgcggtaa
    22201 tgcgggaaca gatgctccat ctgcgggagc ttggcgctgg gcttcatgtc accttgcagg
    22261 tactgccgtt cgccagcggc gcccacgctg cggcggtcgg caacttcctt gtgctcggcg
    22321 gccccacgga ggatctggac gtcgtgtacg tagacatcct cggcggcggg ttgttcatgg
    22381 agaagccggc agaactgaag cgctataaga tgacgttcga gtacttgcgc gcacaggcgt
    22441 tgagcatcga ggcgtcgagc gcgttcatcg atcgcgtctg cgaggagctt tgatgcgcga
    22501 ggtaacgtca cccgcccctt ctccgcaccc cta
//
