LOCUS       NZ_CP045178            10399 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1726006
            Orig. end                         :: 1736405
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1162..2262
                     /locus_tag="GBW32_RS07620"
                     /old_locus_tag="GBW32_07620"
     CDS             1162..2262
                     /NRPS_PKS="Domain: Aminotran_5 (74-338). E-value: 1.3e-29.
                     Score: 95.2. Matches aSDomain:
                     nrpspksdomains_GBW32_RS07620_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989360.1"
                     /locus_tag="GBW32_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07620"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_077972074.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.5e-23, bitscore:
                     80.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTASHFANIERRVAALLSTDQDVVIMQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDCGATVYDLAVPFRTAVTAQQVREALAER
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMVDAVASVGAEPLLPKAWGVDLCV
                     IGAQKALGGPAGVSAVTVSDRAWERLAANPYAPRKSYLSLLDWKHRWIDTGRRALPHAP
                     AQLEMLALEASVEQFEKEGAETVTARHASAASATRAGALALGGGVAPYVHEARDAAPVA
                     TTLRVPEGTDASEVVARALTAEPALPLVAGGGVLAKEMIRVNHYGPAATRGVVRASVAA
                     LGGALAELGVTVDLSAARRAVEESWR"
     aSDomain        1384..2175
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS07620_Aminotran_5.1"
                     /evalue="1.30E-29"
                     /label="GBW32_RS07620_Aminotran_5.1"
                     /locus_tag="GBW32_RS07620"
                     /protein_end="338"
                     /protein_start="74"
                     /score="95.2"
                     /tool="antismash"
                     /translation="RDCGATVYDLAVPFRTAVTAQQVREALAERPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFMVDAVASVGAEPLLPKAWGVDLCVIGAQKALGGPAGVSA
                     VTVSDRAWERLAANPYAPRKSYLSLLDWKHRWIDTGRRALPHAPAQLEMLALEASVEQF
                     EKEGAETVTARHASAASATRAGALALGGGVAPYVHEARDAAPVATTLRVPEGTDASEVV
                     ARALTAEPALPLVAGGGVLAKEMIRVNHYGPAATRGVVRASV"
     misc_feature    complement(2340..2355)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
     misc_feature    2493..2508
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.76"
                     /tool="antismash"
     misc_feature    2493..2511
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.16"
                     /tool="antismash"
     misc_feature    complement(2495..2509)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    complement(2496..2511)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.78"
                     /tool="antismash"
     misc_feature    2613..2624
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.34"
                     /tool="antismash"
     misc_feature    complement(2619..2634)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2629..2644
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            2800..3393
                     /gene="ectA"
                     /locus_tag="GBW32_RS07625"
                     /old_locus_tag="GBW32_07625"
     CDS             2800..3393
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="GBW32_RS07625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07625"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_077971943.1"
                     /transl_table=11
                     /translation="MTAAQADLARARTEFREMPEGLRLDGPEVEDGATIWRIARDSKTL
                     DLNSSYSYLLWCRDFAGTSVVARDSGGDPVGFITGYIRPDRPQTLVVWQVAVDASQRGR
                     GLAAAMLDGLIDRISARQELVSLETTIAPGNVPSERLFTSFGRRSGATVEREVLFDAGL
                     FPDGGHDPEVLYRIGPLAPGRPPAPEEPAGTGVA"
     gene            3543..4811
                     /gene="ectB"
                     /locus_tag="GBW32_RS07630"
                     /old_locus_tag="GBW32_07630"
     CDS             3543..4811
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 5.5e-74.
                     Score: 241.1. Matches aSDomain:
                     nrpspksdomains_GBW32_RS07630_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="GBW32_RS07630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07630"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_077971941.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.3e-76, bitscore:
                     253.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLYDEDDHTY
                     LDFFSGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLESFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALSELCKRQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALDSYWADGHMEKQTLARGEQVESALR
                     AICNEHTQLGASYRGRGLVWGLEFEEPSRAAAVCARAFEHHLLLETSGPQSEVVKLLPA
                     LTVTPEELDEGLRILARSVRETA"
     aSDomain        3627..4637
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS07630_Aminotran_3.1"
                     /evalue="5.50E-74"
                     /label="GBW32_RS07630_Aminotran_3.1"
                     /locus_tag="GBW32_RS07630"
                     /protein_end="365"
                     /protein_start="28"
                     /score="241.1"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDDHTYLDFFSGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLESFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALSELCKRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSIGGYGMPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALDSYWADGHMEKQTLARGEQVESALRAICNEHTQLGASYRGRGLVWGLEFE"
     misc_feature    4827..4842
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     misc_feature    complement(4995..5011)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.23"
                     /tool="antismash"
     gene            5001..5399
                     /locus_tag="GBW32_RS07635"
                     /old_locus_tag="GBW32_07635"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="GBW32_RS07635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07635"
                     /product="ectoine synthase"
                     /protein_id="WP_077971939.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-53, bitscore:
                     178.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDVEGTDRHIKAESGTWESKRIVLAKEKVGFSLHETTMY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGEKYWITPGTMYLLNGHEHHTMRPKSDFR
                     CVCVFNPPVTGREEHDENGVYPLLTEEA"
     gene            5403..6299
                     /gene="thpD"
                     /locus_tag="GBW32_RS07640"
                     /old_locus_tag="GBW32_07640"
     CDS             5403..6299
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="GBW32_RS07640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07640"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_077971937.1"
                     /sec_met_domain="PhyH (E-value: 1e-35, bitscore: 122.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVIPEVTDLYPSRGATEVAVPRQDPVVWSEPGAKGPVSASDLQS
                     FERDGFLSIGELIGPDEVSVYQAELDRLISDPAIRADERSIVESQSKEIRSVFEVHKIS
                     EVFARLVSDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRAVSVSVALTENFDTNGGLMIMPGSHKTFLGCSGETPKDNYKKSLQMQDAGTPSDEAL
                     TKLASEHGIKLFTGKAGSATWFDCNAMHGSGDNITPFARSNVFIVFNSVENEAVEPFSA
                     PVRRPEFIGARDFTPVR"
     gene            complement(6480..7565)
                     /locus_tag="GBW32_RS07645"
                     /old_locus_tag="GBW32_07645"
     CDS             complement(6480..7565)
                     /NRPS_PKS="Domain: Aminotran_5 (27-304). E-value: 1.5e-25.
                     Score: 81.8. Matches aSDomain:
                     nrpspksdomains_GBW32_RS07645_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567664.1"
                     /locus_tag="GBW32_RS07645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07645"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_107502987.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.1e-20, bitscore:
                     72.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGKASGPHSGAMDRDTLAPSEFAPRTTYLNTASTGLLPARAAAA
                     VTEATNAMAAGLSADMFEDVEAARASFARLAGVPARRVAAGASVAVHAGLVAASLPDGA
                     EVLVAEGDFSSLVNPFHVRRDLKVRTVPLESLAGAVRPDTALVAVSSVQSADGRRADLD
                     AIGAAARAYGARTLVDASQSVGWLPLEASAYDYTVAVAFKWLMGPRGVAFMVVPDNLGG
                     LTPTSAGWVSGERPWDSCYGQITELAHSARRFDESPSLLAYVGARRSLELVEEVGVAAI
                     GAHDLALAERYRTGLAELGHEPVAAPGSPIVSVPGLGHRQGELSRAGVQVSDRAGNLRT
                     AFHLYNAPADVDRLLEVLAEG"
     aSDomain        complement(6654..7484)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS07645_Aminotran_5.1"
                     /evalue="1.50E-25"
                     /label="GBW32_RS07645_Aminotran_5.1"
                     /locus_tag="GBW32_RS07645"
                     /protein_end="304"
                     /protein_start="27"
                     /score="81.8"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARAAAAVTEATNAMAAGLSADMFEDVEAARASF
                     ARLAGVPARRVAAGASVAVHAGLVAASLPDGAEVLVAEGDFSSLVNPFHVRRDLKVRTV
                     PLESLAGAVRPDTALVAVSSVQSADGRRADLDAIGAAARAYGARTLVDASQSVGWLPLE
                     ASAYDYTVAVAFKWLMGPRGVAFMVVPDNLGGLTPTSAGWVSGERPWDSCYGQITELAH
                     SARRFDESPSLLAYVGARRSLELVEEVGVAAIGAHDLALAERYRTGLAELGHEPV"
     gene            complement(<7741..7824)
                     /locus_tag="GBW32_RS36345"
                     /old_locus_tag="GBW32_07650"
                     /pseudo=""
     CDS             complement(<7741..7824)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671937.1"
                     /locus_tag="GBW32_RS36345"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07650"
                     /product="DsbA family oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTRFERALAGFPHLEQVEVVHRSFWLDP"
     gene            7950..8258
                     /locus_tag="GBW32_RS36350"
                     /old_locus_tag="GBW32_07655"
     CDS             7950..8258
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF045158.2"
                     /locus_tag="GBW32_RS36350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07655"
                     /product="DUF6879 family protein"
                     /protein_id="WP_405518414.1"
                     /transl_table=11
                     /translation="MTNPVLEGLAKARRSAVHLEMRDAYQANDPGFIAWQNGKRLDPTA
                     DRSEWWRPFLDVVTETTARGVIMVSGLLAGSLPRPAPDVRVHRGRVARCPAETGMRR"
     gene            <8155..8643
                     /locus_tag="GBW32_RS07660"
                     /old_locus_tag="GBW32_07660"
                     /pseudo=""
     CDS             <8155..8643
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008749503.1"
                     /locus_tag="GBW32_RS07660"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07660"
                     /product="DUF5753 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="FRAYSPGLFPGLLQTFGYTEDVLRAVQRRRVCVDDVADAVAVRME
                     RQQVLHDGHRRFAFLVEESMLRNGLGDAETQAEQLDHLTDIAAPANVGVGVVPTRLGRT
                     RMPVEGFGIFDKGQVNVELVSGYLTITQPSEVTAYADAFATLADMAKATPFNAPRMNG"
     gene            complement(9166..10155)
                     /locus_tag="GBW32_RS07670"
                     /old_locus_tag="GBW32_07670"
     CDS             complement(9166..10155)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010275087.1"
                     /locus_tag="GBW32_RS07670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_07670"
                     /product="DMT family transporter"
                     /protein_id="WP_227025041.1"
                     /transl_table=11
                     /translation="MRTSRTRSEAGTDPGQTGPTPGGPVPGGAVQDGIPVRRPLAPAAL
                     VLGQIVSLQVGSAVAKQSYGEIGPTAMAGMRLGFAALVMYALVRPRLRGLSAPQWRAAC
                     GLGAVLAAMNVAYFQAIARLPLGIAATVELLGPLLLALALSRRPAHLAAAGLALVGVLL
                     LAAPGGSLPLTGMVCAAAAALGRALYILLNRRVGLLYDNWAGLSVALLTGACLLTPVAA
                     FSGAAATVAARPALLWSGLLVAVCSSLIPYSLDMLALRRIGARSFGVLLSLSPAVGAAV
                     GFAFLGEHLTARQGVALGLVLAGALWSARVPGRARGGPAPTEGAEKSP"
ORIGIN
        1 cccaggtggc catcgccagg gcccgcaggg gcgggattcc ggcccgggtg agctgctcga
       61 cctcggcggc gaccagtccg tgcggcagcc cgccgcccgc gtccgtaccg gcgtagatcg
      121 gcaccccggc gtcgtaggcg gcgcgcacgg tgtcgtagcg ccgctcgtgc agcctgcgca
      181 tatgggccga ccagcggggg aacttggccg cgccgccctc ggcgagccgc gggaacgtgg
      241 cgatgttgac cagcgtgggc acgatggcga ccccgcgctc cgcgaagagc gggatggtct
      301 cctcggtgag ccccgtcgcg tgctcgatac agtcgatgcc cgcctcgacc aggtcggtca
      361 gcgagtcctc ggcgaaacag tgcgcggtga cccgtgcgcc gtggcggtgg gcctccgcga
      421 cggcctcctc cagggcgccg cgcggccagc acgcggagag gtcgcccgac tcgcggtcga
      481 tccagtcgcc gacgagcttg acccatccgt cgccgcgccg cgcctcctgc cgtacgtacg
      541 cgacgagatc gcccggctcg atctcgtggg cgtagttgcg aatgtaacgg cgggtcctcg
      601 cgatgtgccg gccggccctg atgatcttcg gcaggtcctc gcggtcgtcg atccaccggg
      661 tgtcggaggg cgaacccgcg tcacgcagca gcagcgcccc gatgtcccgt tcggtcagcg
      721 cctgcttctc cgacacctcg tcgggtacgg gaccgtggct gtcgagtccg acgtggcagt
      781 gcgcgtcgac caggccgggc agcgcccacc cctcgacggt acgtacgtca cgggcgagcg
      841 ccggtctgtc gtaggtgacg cggcccccca cgacccacag ttcctcgcgc acctcgtcgg
      901 ggccgacgag cacgcctccc ctcacgtgca gcacggcctg atcactcatg tacggcaccc
      961 taaagggttc ttgacgggga ctgccgctcg aaggtccatg gcctgccgta cggggggtcg
     1021 cggactgccg tacgggtgcc tcgggcgccc acgcccctgg cgtaccgcac ttccacgcgg
     1081 gggccgcgaa cacggtgagt actctcggga acggcagcca gaacgaaccg agacgaactc
     1141 gtgagcgaag agagcaccac tgtgacgcac cccctcctcg acctgacccc gctgaccgca
     1201 tcgcacttcg ccaacatcga gcggcgcgtg gcggcgctgc tctccaccga ccaggacgtc
     1261 gtgatcatgc agggtgaggc gctgctgccc ctggaagggt gcatccgcgg tgtcgcggga
     1321 cccgacacga cagcgctcaa cgtgatcacg ggaccctacg gccagacgtt cggtgactgg
     1381 ctgcgggact gcggcgccac cgtgtacgac ctggccgttc ccttccgtac ggcggtcacg
     1441 gcgcagcagg tgcgcgaggc gctggccgaa cgccccgaga tcgacttcgt gtcgctggtg
     1501 cacgcggagg cggccacggg caacaccaat ccggtcgcgg agatcggtga ggtggtgcgg
     1561 gcgcacggtg cgctgttcat ggtggacgcg gtggcgtcgg tcggcgcgga gccgctgctg
     1621 ccgaaggcgt ggggcgtgga cctgtgcgtg atcggcgcgc agaaggccct tggcggtccc
     1681 gcgggcgtgt cggcggtgac ggtgagcgac cgtgcgtggg agcggctggc cgccaacccc
     1741 tacgcgcccc gcaagtcgta cctgtcactc ctggactgga agcaccgctg gatcgacacc
     1801 ggccgccggg ccctcccgca cgctccggcg cagctggaga tgctggccct tgaggcgagt
     1861 gtggagcagt tcgagaagga gggcgcggag acggtgaccg cgcggcacgc gagcgcggcc
     1921 tcggccaccc gcgccggcgc cctcgccctg ggcggcggtg tcgctcccta cgtccacgag
     1981 gcgcgggacg cggcaccggt ggcgacgacg ctccgggtgc ccgaggggac ggacgcctcc
     2041 gaggtggtgg cacgggccct gacggcggag cccgccctgc cgctggtggc gggtggcgga
     2101 gtgctggcca aggagatgat cagggtgaac cactacggtc ccgcggccac ccgaggtgtg
     2161 gtgcgggcct ccgtggcggc gctgggcggg gccctcgccg agctgggcgt caccgtggac
     2221 ctctcggcag cgcggcgggc ggtcgaggag agctggcgct aggcccgacg cggtcaaaac
     2281 ggccgcctcc gggcgcgggg gcgggccgtg cggcgtggtt tcgccgcacg gcccgcccct
     2341 tttcgcgttc agcgtgccgc gcgcccggcc gcgttcggcg ctgaccgggg ggccgtccct
     2401 gtcacccctg ccgtgctctt tcgcgccact cgccgcgcca ctctcggact caagccgcgg
     2461 tcgcacagaa ttcccatgcc ttagccatag cgaatgagag cgaatatcat caactaatcg
     2521 atcacagctt cccgtattct tctgcccgct ttccccgacc ctaaaccgcc aagatttcgc
     2581 gaccaccaat cggcgtaatt cggacgcatg tcgaaagcgt tacccacatg tgacacactc
     2641 cacaccgagt ccgctttgca ccttattacc accacaaatc atcggttttc accccccgtt
     2701 cgcgcgagca cgcaccctcg cccgttaaca catcaggcgc tttcatgtaa ccccactcct
     2761 cgatgcaatt tcaagaagtg ctcggtaaat tcaattccta tgaccgccgc acaagcagac
     2821 cttgcacgtg cccgtaccga attccgggaa atgccggagg gactacggct cgacggcccg
     2881 gaggtggagg acggagccac gatctggcgc atcgcccggg actccaagac cctcgacctc
     2941 aactcctcgt acagctacct gctgtggtgc cgtgacttcg ccggtacctc tgtggtggcg
     3001 cgcgactccg gtggcgaccc ggtcgggttc atcacgggct acatcaggcc cgaccgacca
     3061 cagaccctcg tcgtctggca ggtcgccgtg gacgcgtccc agcgtgggcg cggtctggcg
     3121 gccgcgatgc tggacgggct gatcgaccgg atctccgcca ggcaggagct ggtctcgctg
     3181 gagaccacca tcgcgccggg caatgtccca tcggaacgcc tcttcacctc cttcgggcgg
     3241 cgctcgggcg ccaccgtgga gcgtgaggtc ctcttcgacg ccgggctctt ccccgacggg
     3301 ggccacgacc ccgaagtgct ctaccgcatc gggccactcg cgccgggcag gccaccggcc
     3361 ccggaagagc cggccggaac cggcgtggcc tgagcctcgg ggctccgtga ggccgccgct
     3421 gcaccgccgt gaccgggcgg acgcgtcggc cggcacgttc cgttcccgga tgacgacacc
     3481 ctgtcgcccc ctgtccgcac ccccccacac ttttcgcatc ccaatctccc aggagagatg
     3541 ctgtgaccat cacaccgccc gccctgagtg tcttcgagac cctggagtcg gaggtgcgca
     3601 gctactgccg cagttggccc gccgtcttcg acagggcaca gggcagctac ctctacgacg
     3661 aggacgacca cacctacctc gacttcttct ccggcgccgg ggccctcaac tacgggcaca
     3721 acaacccggt tctcaaacgc gcgctgatcg actacatcga gcgtgacggc atcacgcacg
     3781 gcctcgacat ggggaccacg gccaaacgcg cgttccttga gtccttccag aacgtcatcc
     3841 tgcgcccccg cgacctgccg tacaaggtca tgttccccgg cccgacgggc accaacgccg
     3901 tcgaggccgc gctgaagctg gcgcgcaagg tcaagggccg cgagtcgatc gtctccttca
     3961 ccaacgcctt ccacggcatg tcgctcggct cgctcgccgt gacgggcaac gccttcaagc
     4021 gcgcgggggc cggcatcccg ctggtgcacg gcaccccgat gccgttcgac aactacttcg
     4081 acgggaccgt cgaggacttc ctctggttcg agcgtctcct tgaggaccag gggtccgggc
     4141 tcaacaagcc cgccgccgtc atcgtggaga ccgtgcaggg cgagggcggc atcaatgtcg
     4201 cccggcccga gtggctgcgc gccctctccg agctgtgcaa gcgccaggac atgctcctga
     4261 tagtcgacga catccagatg ggctgcggca ggaccggcgc gttcttctcc ttcgaggagg
     4321 ccggcatcac gccggacatc gtgacgctgt ccaagtccat cggcggctac ggcatgccca
     4381 tgtcgctctg cctgttcaag ccggagctgg acgtctggga gcccggcgag cacaacggca
     4441 ccttccgcgg taacaacccc gcgttcgtca ccgccgccgc cgcactcgac tcgtactggg
     4501 ccgatgggca catggagaag cagaccctgg cccgcggcga gcaggtcgag tcggcgctgc
     4561 gcgcgatctg caacgagcac acccagctcg gcgcctccta ccggggtcgc ggcctggtct
     4621 gggggctgga gttcgaggag ccctcgcgtg ccgccgccgt ctgcgcgcgc gccttcgagc
     4681 accacctgct gctggagacc tcggggccgc agagcgaggt cgtcaagctg ctccccgcgc
     4741 tgaccgtgac gccggaggaa ctggacgagg gtctgcggat cctggcccgc tccgtacgcg
     4801 agacggcctg accgcccgag caccacgaca cggtccggtc accgacgggc ccgccgggcg
     4861 acggcatcgg ctgccgtccg gcgggtccgt gggccaccgg ccccggcccc gcgcgtacgc
     4921 ggatacacgg atacacggat acgcgtacgg ggtcataagt gaatattcag ggttacccga
     4981 gagagaggcg ctgacgcacc gtgattgtcc gatcgttcaa ggacgtcgaa ggaaccgacc
     5041 gccacatcaa ggccgagtcg ggtacttggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtcggctt ctccctgcac gagaccacca tgtacgcggg caccgagacc tccatgtggt
     5161 acgcgaacca catcgaggcc gtgctctgcg tcgagggtga agccgagctg accgatgacg
     5221 agaccggtga gaagtactgg atcacaccgg gaacgatgta tctgctcaat ggccatgagc
     5281 accacaccat gcggcccaag agcgatttca ggtgcgtatg tgtcttcaac ccgcccgtca
     5341 ccggacggga ggagcacgat gagaacggcg tctacccact gctgacagag gaggcctgag
     5401 agatgaccgt cataccggaa gtcaccgatc tctatcccag ccgcggcgcc accgaggtgg
     5461 cagtcccccg tcaggatccg gtcgtgtggt ccgagcccgg ggccaagggc cctgtctcgg
     5521 cttccgacct gcagtcgttc gagcgggacg gctttttgtc gatcggtgaa ctgatcggtc
     5581 ccgacgaggt ctccgtctac caggcggagc tggaccggct gatctccgac cccgcgatca
     5641 gggccgacga gcgctcgatc gtggagtcgc agagcaagga gatccggtcg gtcttcgagg
     5701 tgcacaaaat cagcgaggtc ttcgcccggc tggtgagcga cgaacgggtc gtgggccgcg
     5761 cgcggcagat cctcggctcc gacgtctatg tgcaccagtc caggatcaat gtgaagcccg
     5821 gcttcggcgc ctccggtttc tactggcact cggacttcga gacctggcac gccgaggacg
     5881 gtctgcccaa catgcgcgcg gtgtccgtct cggtcgcgct gaccgagaac ttcgacacca
     5941 acggcgggct catgatcatg cccggttcgc acaagacgtt cctcggctgc tccggtgaga
     6001 cgccgaagga caattacaag aagtccctcc agatgcagga cgcgggcacg ccctccgacg
     6061 aggcgctgac caagctcgct tccgagcacg gcatcaagct cttcacgggc aaggccggct
     6121 cagccacctg gttcgactgc aacgcgatgc acggctcggg cgacaacatc accccgttcg
     6181 cccgcagcaa tgtcttcatc gtgttcaaca gcgtggagaa cgaggccgtg gagccgttct
     6241 ccgcgccggt gcggcggccc gagttcatcg gggcgcgcga cttcacgccg gtacggtgag
     6301 aaccgggcgc cgtcgccgga cgcccgccga cccgacaggg cgcggcgtgg ggcggagcgg
     6361 cgcccgccac tgaggcgggg gcgggaccgg agcgatccgg gccccgcccc cgcctccgtg
     6421 ctgtacggct gctgcgtgcg gggcggtcgc tctcgtacgg tgcttcggtg ctgcgtggct
     6481 cacccctccg cgagcacctc aagcagtcgg tccacgtcgg ccggtgcgtt gtagaggtgg
     6541 aaggcggtgc ggaggttgcc cgcccggtcg gacacctgca cccccgcacg gctcagctcg
     6601 ccctggcggt ggccgagccc tggcacggag acgatgggtg agccgggggc ggccaccggc
     6661 tcgtgaccca gctcggcgag gcccgtcctg taccgctcgg cgagggcgag atcgtgggcg
     6721 ccgatggctg ccacgcccac ctcctccacc agctccagcg acctgcgcgc accgacgtag
     6781 gcgagcaggc tcgggctctc gtcgaaccgc cgtgcggagt gggcgagttc ggtgatctgc
     6841 ccgtagcagc tgtcccaggg ccgctccccc gagacccatc cggcggaggt cggggtgagc
     6901 ccgccgaggt tgtcggggac gaccatgaag gcgaccccgc gcggacccat cagccacttg
     6961 aaggcgacgg ccacggtgta gtcgtacgca ctcgcctcca gcggcagcca cccgacggac
     7021 tgcgaggcgt ccacgagggt gcgggcgccg tacgcgcggg ccgccgcgcc gatcgcgtcg
     7081 aggtcggcgc ggcggccgtc cgccgactgg acggaactca ccgcgacaag ggcggtgtcc
     7141 ggccgcaccg ccccggccag ggactccagg ggtacggtgc gcaccttgag gtcacggcgc
     7201 acgtggaacg ggttcaccag cgaactgaag tcgccctcgg cgacgaggac ttcggccccg
     7261 tcaggcaggg aggcggcgac cagcccggcg tggacggcga ccgaggcgcc cgccgcgacc
     7321 ctgcgcgcgg gcactcccgc gagcctggcg aaggaggcac gggcggcctc cacgtcctcg
     7381 aacatgtcgg cggacagccc cgcggccatg gcgttcgtcg cttcggtcac ggcagccgcg
     7441 gcgcgggccg ggagcagtcc tgtgctcgcg gtattgagat acgtggtcct gggtgcgaac
     7501 tcgcttggtg ccagagtgtc tctgtccatg gctccactgt gcggtcctga cgctttcccg
     7561 tccatcacct ctccacgtac gggctctcca agtgcccctg gggggcggag ggcggtaggg
     7621 ccgtggggcc cggaatgcgg gatgcggatc ggggaccgtg agcactgtca gagccgcgcg
     7681 gctgaaccca gccagagcgc ctacctgaac cagggccaga ccgactgccg ccaccacggc
     7741 gggatcgagc cagaaggacc gatgcaccac ctcgacctgc tcaaggtgtg ggaaacccgc
     7801 gagagccctc tcgaagcggg tcagccctca gcttgccggg ccgcgtacgt cttgacggcc
     7861 acccacggac gttccggatc atgaagtgat catccgtctc cccaaggtca tgcggcagta
     7921 tgtgccgccc cacgacagtg agtaggacta tgacgaaccc tgtgcttgag ggactggcga
     7981 aggctcgccg ctccgctgtc cacctggaga tgcgggacgc ctaccaggcg aacgaccccg
     8041 ggttcatcgc gtggcagaac ggcaagaggc tggaccctac ggccgaccgc tccgagtggt
     8101 ggcgtccgtt cctggacgtg gtgacggaga cgacggcgcg gggcgtgatc atggtttcgg
     8161 gcctactcgc cgggtctctt ccccggcctg ctccagacgt tcgggtacac cgaggacgtg
     8221 ttgcgcgctg tccagcggag acgggtatgc gtcgatgacg tggccgacgc cgtagccgtc
     8281 cgcatggaac gtcagcaagt gctccacgac gggcaccggc ggttcgcgtt cctggtcgag
     8341 gaatccatgt tgcgcaatgg gcttggcgac gctgagacgc aagcggaaca gctcgaccac
     8401 ctgacggaca ttgccgcgcc ggccaacgtt ggcgtcggcg tggtgcctac tcgactcggc
     8461 cggacccgta tgccggtaga gggctttggg atcttcgaca aggggcaagt gaacgtagag
     8521 cttgtgtccg gctacctcac catcacgcag cccagtgagg tgaccgccta cgccgacgcg
     8581 ttcgccacgc tggccgatat ggccaaggca acgccattca acgcaccgag gatgaatggc
     8641 tgaccattgc tcggtacgtc aggcacgctg ccaacaagct cgggccggaa ctcccgctgt
     8701 gcctccctgg tgaaccgcgg gaatgcgggc ggacggctca acagcacgtg atcgcatggg
     8761 ccgctcacct tcgggcggtg tcccatcacc tgatcgaaca ggccaccccg agcgaggcca
     8821 ggggtgcaca tgccatcggc ccgctgtacc aacgtcgtct tgccgagttg cgcgcgtcca
     8881 cctccgtgcc gcactgaacg acagcaggcc ccgacgggcc ccgttcgcga cgcttccccc
     8941 tggcgtccgg gcagggcctc tcgcgttcat cgctgctgct cagagaccac gcgctaagga
     9001 cacggctcgt ccgggcacag cccttgcggg gacagctcgt acgggcgcgg aggcaccctc
     9061 cgctcctggt cacaacatgt cgcgtcgcgg gcccgttccc ggcctccgag ccagcgcggg
     9121 cccgtacttc agttcccgca gaacgcactc gccgccatgt gttggctacg ggctcttctc
     9181 cgcaccctcc gtcggcgccg ggcccccgcg cgcccggccg gggacccggg ccgaccacag
     9241 cgccccggcc aacaccagac ccagcgccac tccctgccgc gccgtgaggt gttcgcccag
     9301 gaaggcgaag ccgacggccg cgccgacggc tgggctcagg gagagcagta cgccgaagga
     9361 gcgcgcgccg atgcggcgca gtgccagcat gtcgagcgag tacgggatga gcgaggagca
     9421 gaccgccacc agcagtccgg accacagcaa cgccggtcgc gcggccaccg tggccgcggc
     9481 gccactgaac gcggccacgg gagtgagcag gcacgctccg gtcagaagcg ccaccgagag
     9541 ccccgcccag ttgtcgtaca gcaggccgac ccgccggttc agcagaatgt agagcgccct
     9601 gccgagcgcc gccgccgcgg cgcagaccat gccggtcagc ggcagcgagc cgcccggggc
     9661 cgcgagcagc agtactccta cgagtgccag cccggcggcg gcgaggtggg cggggcggcg
     9721 ggagagggcg agggcaagca ggagcgggcc gaggagttcc accgtggcgg cgatcccgag
     9781 cggcagccgt gcgatggcct ggaagtaggc gacgttcatc gcggcgagaa cggcgccgag
     9841 cccgcacgcg gcccgccact gcggcgccga gagtccgcgc aggcggggcc gtacgagggc
     9901 gtacatcacg agggcggcga agccgagccg catcccggcc atggccgtgg gcccgatctc
     9961 gccgtaggac tgtttggcga cggccgaacc gacctgaagg ctgacgatct gcccgaggac
    10021 cagcgcggcg ggagccagtg ggcggcgtac cggtatcccg tcctggacgg ctccgcctgg
    10081 aacgggtccg cccggggtgg gcccggtctg ccccgggtcc gtgccggcct cacttcgtgt
    10141 acgggatgtt ctcactgggt accggtcccc tcgcccctct ccggcccggg gcacgggagg
    10201 cgccgcgtga ccggcggctt tcgccgtacg gctgaggtcc cagcgtgatg tcatgtcctt
    10261 atgcagttca accattacgg tggcgaggcg gccaggctcg cggccgacct ggccaacctc
    10321 accgcgccgc cgcaccccca cgagtgggag ccgctgctcg ccgcgcgcgg ggtgcgggaa
    10381 cgtgacctgt cggacagcg
//
