LOCUS       NZ_CP045178            21236 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 25216
            Orig. end                         :: 46452
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21236
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="atropopeptide"
                     /region_number="2"
                     /rules="atropopeptide_p450"
                     /tool="antismash"
     cand_cluster    1..21236
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="atropopeptide_p450"
                     /kind="single"
                     /product="atropopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21236
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11236](-)"
                     /cutoff="10000"
                     /detection_rule="atropopeptide_p450"
                     /neighbourhood="10000"
                     /product="atropopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11236)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="atropopeptide_p450"
                     /neighbourhood="10000"
                     /product="atropopeptide"
                     /protocluster_number="1"
     misc_feature    complement(395..404)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            731..1816
                     /locus_tag="GBW32_RS00195"
                     /old_locus_tag="GBW32_00195"
     CDS             731..1816
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131466.1"
                     /locus_tag="GBW32_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00195"
                     /product="alkene reductase"
                     /protein_id="WP_077972222.1"
                     /transl_table=11
                     /translation="MPTQPLLQPVRLGALELANSVVMAPMTRARAQNAELAPTDLHATY
                     YAQRAGAGLIVTEGTWVSPDAIGFINVPGIYTDTQTAGWAKVTEAVHAAGGRIVSQFGH
                     LGAASHPDHFGGRLPAGPSAINPGEKSFTPSGPKDTLTPRAYTAAEIADTIADYRHAAE
                     NARRAGFDGVELHAQGPHLIPQFLNPRLNQRTDAYGGSSEKRAKFLLDVLDAVTDVWGS
                     DRIGVKMSPGWTSGTAFTAAEETLADYDQLLKKLNDSDLAYLHLLGTAGTTEERIALFS
                     RYRAHYLGNIVANLGFTQTLGNEILDRGIVNAVSFGAPFIANPDLVERFTQGHPLADGD
                     RDTYYAGHGEGYTDYPAFTAG"
     gene            complement(2340..2594)
                     /locus_tag="GBW32_RS00200"
                     /old_locus_tag="GBW32_00200"
     CDS             complement(2340..2594)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436428.1"
                     /locus_tag="GBW32_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00200"
                     /product="hypothetical protein"
                     /protein_id="WP_077972220.1"
                     /transl_table=11
                     /translation="MATYETEIHTGGGGWQQDEPLSISLAGRGDVVPANGAPATGTSVT
                     WSGEAGNGSVTFFDNGGSFQGTAQFPGEGPVGYRGTKTD"
     gene            3042..3335
                     /locus_tag="GBW32_RS00205"
                     /old_locus_tag="GBW32_00205"
     CDS             3042..3335
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018554927.1"
                     /locus_tag="GBW32_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00205"
                     /product="hypothetical protein"
                     /protein_id="WP_179120284.1"
                     /transl_table=11
                     /translation="MTAFGREPAEVRIPRAALDALAAALSVRTVAMRTWPDGIEWMYPM
                     GTWDEPHLEVALMPGGEEVWLRMSTDRSSVAVWTIQQWLAFTRKLPGATPPD"
     gene            complement(3441..5660)
                     /locus_tag="GBW32_RS00210"
                     /old_locus_tag="GBW32_00210"
     CDS             complement(3441..5660)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015210.5"
                     /locus_tag="GBW32_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00210"
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_179120283.1"
                     /transl_table=11
                     /translation="MAADEISSQGFNLNELFRDVTYEIDYYQRDYTWGEEEVRTLLRDL
                     CDSFRNWSGDSVYRRRPQTAPQYFLGPFVYHEPVRNRRFLVDGQQRFVTLHVLFLQLRL
                     WAREMGDSQTVDQLNRVITTDGKSFCVGITDHDPVLRAVSEGRKYETGAGDSLSRRNLW
                     ARNQQIEYQLIEELDAEELRHFTEWLLKRVVLVGIRAAGPNHGYRMFETMNDRGARLTA
                     VDLLKSHLLSNVGDGEEQLNTQWQEMLRELSTDRDDPLAASHFIKAYLLARCVRQGHAG
                     DRHQVATNLNVWVRRNATHLGLVTGHPDRFLTFVQDLLKSARQYRPVLAATRALKKDGD
                     HLETVLFNERNGLGIQSVAVLAAFDPGDQPTDAKDKGRLVAGYIDRWYALRILQDLPVQ
                     SADLDGLVHNELVPLLRGCRTVADVASELGALAQRDGNPVAEGVTLGLRGNNAHQIRYL
                     LARATAYADEACGKNPDILAYLDRDRFHIEHLWANHHHRVANDIPDPVVFRSVRNQLGG
                     LGLLEGRENSSINDLPFHDKKPLYFRANVLLGVMAPKYDLRNPHLRVFIRTHRLDKHLR
                     TFGVRETMPTVIKTRQELYLSLFERIWDPDRLGLPASAASAPPSDDCGQPPAARRRKAA
                     PRRGAAPRRRTDVAKMIEARILRAGTHIVLTHRSTEHWATIDATGGIILDATGGTAYGR
                     VDEAGAVARGTKTCQGMNEWHIEDEHGVRISLRAVRDRAVATGDL"
     gene            6192..6386
                     /locus_tag="GBW32_RS00215"
                     /old_locus_tag="GBW32_00215"
     CDS             6192..6386
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00215"
                     /product="hypothetical protein"
                     /protein_id="WP_077972216.1"
                     /transl_table=11
                     /translation="MKLHHIAGASLAAAALLACGTQAQAGTGTPAEASVIEGRSLSDPV
                     SCGVSTTATQAAVTACAAS"
     gene            6442..6993
                     /locus_tag="GBW32_RS00220"
                     /old_locus_tag="GBW32_00220"
     CDS             6442..6993
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952800.1"
                     /locus_tag="GBW32_RS00220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00220"
                     /product="phenolic acid decarboxylase"
                     /protein_id="WP_077972214.1"
                     /transl_table=11
                     /translation="MGQNAAASQPPAEHHAPEQDLSGFVGSHFIYTYANGWQYEWYARN
                     EKECDYRIHHGLVGGRWVTRQAVSIVRFSDGQYKADWHEPTGTCVSLLFDLPRRLLHGT
                     VFFPQWIGGEGRHPEKTICYQNEFTDDMHRFRDEGPAYPYVVIPEFAKITYTEQRGRDN
                     DTVIDAAPGQLPDGYADRTN"
     gene            7383..8903
                     /gene="hutH"
                     /locus_tag="GBW32_RS00225"
                     /old_locus_tag="GBW32_00225"
     CDS             7383..8903
                     /EC_number="4.3.1.3"
                     /GO_function="GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hutH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982476.1"
                     /locus_tag="GBW32_RS00225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00225"
                     /product="histidine ammonia-lyase"
                     /protein_id="WP_077972212.1"
                     /transl_table=11
                     /translation="MPTHDASRALLISREALALAHLKTAAEPVDIRVSAEVLDRVAACH
                     TFARRHIDAGNAVYGASTGFGPLVDFRGRDETQQQAENALAHLGAGQGPNLDAPLVRAT
                     MLARLSVLCQGRSGVSTGVVQALAAALGSSFAPAVPSTGSLGASGDLIPLSYVARALKG
                     DGEAYADGTLMPAARALAHCGLTPLELDEREALALVNGTSLTAAAVGLATLSVARSLTV
                     SVLLTALLCDTLGCSTEFASPALLRLYAHPDAERVGAGLRGQLEGIRPDDGRSLQEPYS
                     VRCSPQLIGAVGSTIAYADRVTHDSLNSVTDNPVFFVEDESVIHGGNFFGQSLAFAGDL
                     LSVGSAQLGNLAERQLDALVDPRRNGGRPPMLSPAPGQQHALQGVQLAATAMVVEMRRD
                     TVPASAQSLPTNWHNQDVVPLGTHAALTALRQATSLRLLHGSLAVALRQAVHVGERELT
                     APACVRLYERLADVVDQVDTDRPLGHDVRMAADLLDRIVDEDDGLTSR"
     misc_feature    7411..7425
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.11"
                     /tool="antismash"
     gene            8957..9922
                     /locus_tag="GBW32_RS00230"
                     /old_locus_tag="GBW32_00230"
     CDS             8957..9922
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00230"
                     /product="hypothetical protein"
                     /protein_id="WP_143621460.1"
                     /transl_table=11
                     /translation="MAARTQSVRCHQVAAAPRTGRVENLLLTPSGENDLRCGSPAACDQ
                     PARDDLILLRQAATGTPPVRLLAPGEPDARIRYRWAVGHHAAFGVWRLLAARLRTIADT
                     ASPAGSAITEAARLYDVYSVLFLYTSSCSPERYDATVRADMIACDPAFSGTWSRDHEAV
                     PALMHRMDEAHDAGLTREIKRAARLNGQIHMAVAKKLVPEGVSLLQQEGGPPHRPASAE
                     GDLYDEFFHVRRTATCRCAFSAQILRRLTQVATDMAHHGLHSSDDPLPGLGVKAASGLR
                     RIESSAHQQLLHLAALLASETCRADTGRRGHAGSSFSTHV"
     gene            complement(10001..11047)
                     /locus_tag="GBW32_RS00235"
                     /old_locus_tag="GBW32_00235"
     CDS             complement(10001..11047)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     atropopeptide: atropopeptide_p450"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000172: Biaryl bond formation (64% identity)"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083713.1"
                     /locus_tag="GBW32_RS00235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00235"
                     /product="cytochrome P450"
                     /protein_id="WP_077972207.1"
                     /sec_met_domain="atropopeptide_p450 (E-value: 2e-178,
                     bitscore: 591.4, seeds: 287, tool: rule-based-clusters)"
                     /sec_met_domain="p450 (E-value: 2.2e-12, bitscore: 45.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTGTERLRRETVFAPRIEHLLRSYRGAEAFRLDESTVGIAGAD
                     LMNAVLAARPANEFERPTFKPLQGRKIGRPEASAVMRAVGQDVRTALKTPTDTPVDLTG
                     EWPQVGHRYLRDLVFGADPYRLRVLVDRTLELTPKITWSVIAAGATLPVPPAGRPPLSE
                     LAGLVSSATGYEGRRHAMGMYRRAAAPVCFTVSALVTNALWLGAPFATDTPNEHILLES
                     LRLLPVSWNLLRVADAEFTALDDRIGLADDVLMLPLLSHRDPSHWEDPDVFRPERWATA
                     DPDTHPGYLPFGHVGERCWGRHMVMPLAERLLDLIRRDGLTVDPGQTSAKVPLAGLMGA
                     TGVRVVRG"
     gene            complement(11156..11236)
                     /locus_tag="GBW32_RS37605"
     CDS             complement(11156..11236)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     atropopeptide: atropopeptide_precursor"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF038372.1"
                     /locus_tag="GBW32_RS37605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptorubin family RiPP precursor"
                     /protein_id="WP_218670027.1"
                     /sec_met_domain="atropopeptide_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLSSLKKKIAPEKSLKAYAWYIWY"
     gene            complement(11554..12762)
                     /locus_tag="GBW32_RS00240"
                     /old_locus_tag="GBW32_00240"
     CDS             complement(11554..12762)
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382078.1"
                     /locus_tag="GBW32_RS00240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00240"
                     /product="S8 family peptidase"
                     /protein_id="WP_077972205.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 6.7e-36, bitscore:
                     122.7, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVSMRGAASVLLMVLPMLGGSGAVAAPGPDAVVTQHAAKAVAGE
                     YIVTVKPSFAPKALMRKLDIRPLFTYSTALHGFAAALTPLQLRLARTQPAVSAIEENGR
                     VTLNSAPADGAGGRGRTPTAASEVAAGSWGLDRIDQRRLPLDGRFAVTGTGRGVTAYVL
                     DTGIETGHAEFEGRATVGFDAIGDGRDGQDCHSHGTHVAGTVGGKTYGVAKGVSLVAVR
                     VLDCTGSGTTAQTLAGLDWIATHLKRPAVMNASIGDSASATLDTATNAVADAGALPVVA
                     AGNDAVDACTASPARAQKALAVGATDRQDRQADFSNYGPCLKLYAPGVDIVSAGLGGGS
                     RSLSGTSMASPHAAGGAALLLEQDPGATPATITRLLTDRATTGAVVSPGAGSPDRLLYV
                     GQL"
     gene            13079..15442
                     /locus_tag="GBW32_RS00245"
                     /old_locus_tag="GBW32_00245"
     CDS             13079..15442
                     /EC_number="3.2.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528722.1"
                     /locus_tag="GBW32_RS00245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00245"
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="WP_077972203.1"
                     /transl_table=11
                     /translation="MRALRRAFTALLVATTVATASAASGVANAAERGPKFAHDPTTLVD
                     TSIGNNGDGTTFPGAAAPFGMVQLSPDTQLNKYASYDYAQDTILGFSHTHLSGVGCQTM
                     GNFRFMPTTGAVTSSDPARYAAKFSHDNETRAPGYYGVKFDNGIRAELSATRRTGQHRY
                     TYPAGSGSQNVLIETGESNGSTYAGDIKVVGDDTVEGWLQGGNFCGETGKERYRIFFSA
                     TFDRKFSRFGTWSDDTLTPNQREASRGTKRAGAWLTFDSAKGDQVGTSVGLSYTSVDGA
                     RLNRKAEQPKSFDKARGSAHDAWKDELNRMRVAGGTTADQRTYYSALYHSLLHPSIGSD
                     VDNRYRGFDDKVHRADSTYYQMFSLWDTYRSQNQLVALLNPDETADMAKSVLHVYQDGG
                     WLPRWGLGNGETNVMSGDPVTPWIVDIYNRGLLDKRTSRQLFDALWKNANEVPSDQSLF
                     RGRDGNPTYLENGWVGYQNLPGYTYGDSRQAGSATLEYALADCALSTMADGLGHRGKAS
                     TLAARCDNFAKLWDPGVTSQGFSGFPVTKNADGSVAGDPDPTQAGAFHEGTAWQYQWLA
                     QQDPKTLFGLMGGRARAEQRLDKFFDMPTVLTDPAKAASESWVTGAYDYHNNFAFNPNN
                     EPDFHTPWMYAWTGAPWKTSATLRAMRTLFTDDAYGMPGNDDLGATSSLLVFAMAGVFE
                     AQPGAADYLVTAPMFEKVEIRPEHGRKISVEAPGARASKLQYVSSVHTGKGELRQSWLS
                     HKDLLRSGTIKIKLSAKPSKWGVGSAPPAMGARN"
     gene            complement(15603..16838)
                     /locus_tag="GBW32_RS00250"
                     /old_locus_tag="GBW32_00250"
     CDS             complement(15603..16838)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820095.1"
                     /locus_tag="GBW32_RS00250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00250"
                     /product="chitinase"
                     /protein_id="WP_077972264.1"
                     /transl_table=11
                     /translation="MRRLRALLCAAASTAVAAAGLTALLPSSASGAESSTAALSNRWYA
                     AAPYLMPLDNDPPSATAIMDASGLKAFQLAFILAPNGGGCSPTWDGTAPVSSDTAVGSV
                     ISAIRAKGGDVSVSIGGYGGTKLGQACSDAASTAAAYQQVIDKYGLKAIDFDLEEPEYE
                     NTAAIAREIGAAKILQQKNPGLYVSVTTAGTADGTGWFGKQMLNEAKAQGLTPNNFSIM
                     PFDGGFSGAAAQTAALTAFNGVLRSTFGWSEATAYAHEGFSGMNGRSDTGEFFSQTDFQ
                     TVLDYATSHGMDRFTFWSLNRDRQCDPPDNNGRTSGTCSSVPQSAWDFAKFSVRFAGAT
                     PPTDPPTDPPTDPPTDPGGTCPTTAWSASAVYVKGDRVTQDGHAWEAKWWTTGEKPGTT
                     GEWGAWQDDGPC"
     misc_feature    complement(16903..16918)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.02"
                     /tool="antismash"
     misc_feature    complement(16927..16940)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(16963..17742)
                     /locus_tag="GBW32_RS00255"
                     /old_locus_tag="GBW32_00255"
     CDS             complement(16963..17742)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00255"
                     /product="hypothetical protein"
                     /protein_id="WP_152330718.1"
                     /transl_table=11
                     /translation="MLTEGAHQGDLYSLDQDEGTVGTLVDIGDVLVLPGLLVTLTGLSA
                     GGVTAVRPGDGGASRAIVRQANQLRESAARVRADHLRATEAVVVAWTPLHEARVRAALD
                     RLPVRTLPHASGLRHEGLDTMDAVREAAARTPGRLPGLGRQVTEEVLTALAHTTARACD
                     QAAVHLDAEHPTSWSRPRNAPPHVPCTVSSPRPDRTTSPGASRSPRWTCCAAPTPTPKA
                     SPPWRTSCAAPPRTTRCSKRRPARPTGVARTEPLRET"
     gene            18380..18913
                     /locus_tag="GBW32_RS00260"
                     /old_locus_tag="GBW32_00260"
     CDS             18380..18913
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044105.1"
                     /locus_tag="GBW32_RS00260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00260"
                     /product="hypothetical protein"
                     /protein_id="WP_077972197.1"
                     /transl_table=11
                     /translation="MSSCDPGHAAVGDVGAALMVMDSRLKAVYDGKTATDPEQEALIEQ
                     ITASLGPKGADDVLDGACTLIFMYMKWLRAAYEDQEKDVIEYVVTSLVASMRVMTRSVP
                     PEVIPTMTGLVVAAGTGLSPNLWRKQYGYWTRAEMTPLEVTVVLLAEHINRLTGDRDYA
                     TRLITDALAGTDID"
     gene            19239..19835
                     /locus_tag="GBW32_RS00265"
                     /old_locus_tag="GBW32_00265"
     CDS             19239..19835
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039693.4"
                     /locus_tag="GBW32_RS00265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00265"
                     /product="DUF6221 family protein"
                     /protein_id="WP_077972195.1"
                     /transl_table=11
                     /translation="MGTSAGPPEPAPVIERAEATRLWGREVKDRARRMRIRALELRQRS
                     EQMRSATAGSLSRCRQVNAAFRTARAAASAHGGGAPGRGCGAPAAQWAALVAFVHARLD
                     EEATAADLHHEAPCPTLGGPGRDGVTVCGCRAPAHVRYDVSVRRGIVRDIERALRAADH
                     STPNWPYSELSALVRLKTSALPHELHKQWREAWRP"
     gene            20050..20808
                     /locus_tag="GBW32_RS00270"
                     /old_locus_tag="GBW32_00270"
     CDS             20050..20808
                     /EC_number="4.2.3.153"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569719.1"
                     /locus_tag="GBW32_RS00270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00270"
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="WP_077972193.1"
                     /transl_table=11
                     /translation="MLLLLISPDSVEEALDCVKAAEHLDIVDVKKPDEGSLGANFPWVI
                     REIRDAVPSDKPVSATVGDVPYKPGTVAQAALGAAVSGATYIKVGLYGCTTPEQAVDVM
                     RGVVRAVKEYRQDAFVVASGYADAHRIGCVNPLALPDIARRSGCDAAMLDTAIKDGTRL
                     FDHLPPDVCGEFVRLAHEAELLAALAGSVQVGDLGALTRIGTDIVGVRGAVCEGGDRDR
                     GSIQPGLVAAFRAEMDRHGREHAAAVAAAS"
ORIGIN
        1 ccgcctccct cgccgtgctc gccgacgcac tcggaccgct cgcccacgcc gggaccccgc
       61 aggaggccgc cgcggcggaa atcgtcgtgc tcgcggtcgg ctggccccag gtcccggacg
      121 cggtagccgg gctgccaccc ttcgacggga gcatcgtcat cgacgccacc aaccagttcg
      181 tctccccgcc cccgcacacg aagatcgccg acctgggcga gttgacgggc agcgaatacg
      241 tcgcctcgct gctgtccgga gcccgtgtcg tcaaggcgtt caacaccctc tacggccagt
      301 acatcgccgc cgatccacgc caccgggcag gacgccaggt gctgttcctc gcaggtgacg
      361 acgccgacgc caagacaacc gtcaaggatc tcaccgccgc cttcggcttc gcccccgtcg
      421 acctcggctc cttgcgcgag ggcggacgtc tcatgcagct gggcggtccg ctgtccggcc
      481 tccacgctct caaacaggac tgagccgggc cccgcccgtt cctcgctctt cccctcatac
      541 cccctcaggg gtccttgcaa taggggcgcc cgagccgcgg gggccggcac cgcccctcgc
      601 ggcgttgccg aaaagccctg gtagctccgc cacgaggact ctccggcgcc ttgcgacgca
      661 cggcaccggc ccccgcggcc tgatcggact cccctgttgc aaggacccct taagcacaag
      721 gaatctactg atgcccaccc aacccctgct gcaacccgtc cgcctcggcg cgctggagct
      781 cgccaacagc gtcgtcatgg cccccatgac ccgcgcccgc gctcagaacg ccgagctggc
      841 ccccaccgac ctgcacgcga cctactacgc gcagcgcgcc ggcgccggcc tcatcgtcac
      901 cgagggaacc tgggtcagcc ccgacgcgat cggcttcatc aacgttcccg gcatctacac
      961 cgacacgcag accgccggct gggcgaaggt caccgaagcc gttcacgcgg cgggcgggcg
     1021 gatcgtctcc cagttcggcc acctcggcgc ggcctcccac cccgaccatt tcggcggccg
     1081 cctgcccgcc ggtccctcgg ccatcaaccc cggtgagaag tccttcaccc cctccggtcc
     1141 gaaggacacc ctcaccccgc gcgcctacac cgcagctgag atcgccgaca ccatcgccga
     1201 ctaccgccac gcggccgaga acgcccgtcg ggccggcttc gacggcgtgg aactccacgc
     1261 ccaggggccc cacttgatcc cgcagttcct gaaccctcgt ctcaaccagc gcactgacgc
     1321 ctacggaggc agcagcgaga agcgggccaa gttcctcctc gacgtcctcg acgccgtcac
     1381 tgacgtgtgg ggcagcgacc gcatcggggt gaagatgtcc ccgggctgga ccagcggaac
     1441 cgcgttcacc gcggccgagg agacactcgc cgactacgac cagctgctca agaagctcaa
     1501 cgacagcgac ctggcctacc tgcacctcct gggcaccgcc ggcaccactg aggagcgcat
     1561 cgccctcttc tcccgctacc gcgcccacta cctgggcaac atcgtcgcca acctcggctt
     1621 cacccagacc ctcggcaacg agatcctcga ccgcggaatc gtcaacgcgg tctccttcgg
     1681 cgcacccttc atcgccaacc ccgacctggt cgagcgcttc acgcagggcc acccgctggc
     1741 cgacggcgac cgggacacct actacgccgg gcacggcgag ggctacaccg actaccccgc
     1801 cttcaccgcc ggctgagcaa ggaggaccgc tacgagcagc ggcatccgag caatgctcgc
     1861 aaacggcgaa gtggagaaca gccgcccgtc gtgcatcgtg gagccgaagt cgtgcacggt
     1921 gacgaggcgg gcggtgggac gggccgccgt cctcgaagaa ggacgacggc ccgagcgcca
     1981 gcgggccggt agggctcgcc gatcttgacg acggtgaaga tgatcaccgc actgggccca
     2041 accgacccgg gtgccgggga ctgtgagcag acggtccagt cgcggtatag ggggcggtgg
     2101 ggtggaaggg catccggccg ttttctgcgg ccgcaccttc gcgacgctac cgcccgggac
     2161 cccgcgacgt gacaggaccc gggacgcgct cgtcgccgcg tccggacggg acggccggcg
     2221 cgtgcgtcac gcgcacgacc cgccgccgcc gacatagccg acaccggtgg caccggccgc
     2281 accggcggca ccggcgctgg gtggtatcgc tccgagcgcc gggccgccgt gcaagccggt
     2341 cagtccgtct tggtcccccg gtagccgaca ggtccctcgc cggggaactg ggcggtgccc
     2401 tggaatgagc cgccgttgtc gaagaaggtg accgagccgt tgcctgcctc gccggaccag
     2461 gtgacgctgg tgccggtggc gggggctccg ttcgccggga cgacgtcccc gcggccggcg
     2521 agggaaatac tgagaggctc gtcctgctgc cagcctcccc cgcccgtgtg gatctctgtc
     2581 tcgtacgtgg ccatgtctac tccctggctg atcggtcggc ccgtggtgcg ccgtgacggc
     2641 tcctgtgccg cctgctcaca cactggggcg ccccggtatc cggcggcagg gcgttggcag
     2701 gcaaaccacc cgaccagatg accacgacgg gcatccacgt tgacgcgggg aagcagccga
     2761 agactcaagt cgccgatgtg cggctgtgct ggctccgggg cgggcaaccc cggtgggacg
     2821 acggcgagat gtgatggcga gtgcgccctc gactggctgt ggcgcgacgg gactccgccc
     2881 accggtaccc ggtcgggccg caccttggcg ggccgggggg aaaaacggct cgccgccgga
     2941 tcccgttgga cctgagcgac cgcttcgccg gccgccggcc gtctggcagg gtatgaggga
     3001 gcggtgagct gtcgcgtctc gccgcgggat ggaggagtcc ggtgacagcg ttcggccgtg
     3061 aaccggcgga ggtgcggata ccgcgcgcgg ctctggatgc cttggctgcg gcgctgagcg
     3121 tccgcacggt ggcgatgcgc acctggcccg acggcatcga gtggatgtac ccgatgggga
     3181 cgtgggacga gccgcacttg gaagtggcgc tcatgcccgg cggcgaagag gtatggctgc
     3241 ggatgtccac cgaccgctcc agcgtggccg tgtggaccat ccagcagtgg ctggccttca
     3301 ccaggaagtt gcccggagcg acgccgcccg actgaatcca ctacagggcc gtcagcggcc
     3361 gcggtgcgag gggagcctga ttgcggcgcg gcctgtggag tgatcccttc gctgcccgct
     3421 gccaggtgtc gcacaccggg tcagaggtcg ccggtcgcca ccgcgcgatc acgtacggcg
     3481 cgcaggctga tacgtactcc gtgttcgtcc tcgatgtgcc attcgttcat gccctggcag
     3541 gtcttggtgc cacgggcgac agctccggcc tcgtcgacgc ggccataggc ggtgcctccg
     3601 gtcgcgtcga gaatgatgcc gccggtggcg tcgatggtgg cccagtgctc ggtggaacgg
     3661 tgggtcagga cgatgtgggt gcccgcacga aggatccgtg cctcgatcat cttggccacg
     3721 tccgtgcgtc ggcgcggtgc cgctcctcgg cgcggcgcgg ccttacgacg gcgggcggcc
     3781 ggaggctgac cgcagtcatc cgacggaggt gcggaggcag cggaggcggg caggcccagg
     3841 cggtcggggt cccagatacg ttcgaacagg ctgaggtaca gctcctgccg ggttttgatg
     3901 acggtgggca tggtctctct cacgccgaag gtacgcaggt gcttgtcgag ccggtgtgtc
     3961 ctgatgaaga cgcgcaggtg ggggttgcgg agatcgtact tgggcgccat gacgcccagt
     4021 aggacgttgg cacggaagta gagaggcttc ttgtcgtgaa aggggaggtc gttgatgctg
     4081 gagttctcgc ggccctccag cagcccgagg ccgcccagtt ggttgcggac actgcggaag
     4141 acgaccgggt cggggatgtc gttggccacg cgatggtggt ggttggccca cagatgttca
     4201 atgtggaacc ggtcgcggtc gaggtaggcg agaatgtcgg ggtttttccc gcatgcctcg
     4261 tcggcgtagg cggtcgcacg ggcgaggaga tagcgaatct ggtgggcgtt gttgccgcgc
     4321 agccccagag tgacgccctc agccaccggg tttccgtcac gctgggcgag ggcgcccagc
     4381 tcggaggcga cgtcggccac ggtgcggcat ccgcgaagga gcgggacgag ttcgttgtgg
     4441 accaagccat cgagatcggc ggactggaca ggcaggtcct gaaggatacg cagggcgtac
     4501 cagcggtcga tgtatccggc gaccagacga cctttgtcct tggcgtcggt gggctggtcg
     4561 ccggggtcga aggccgcgag cacggcgacg gactggatgc cgaggccgtt tcgttcgttg
     4621 aaaagcaccg tctccagatg gtcgccgtcc ttcttcagcg cacgggtggc cgcgaggacg
     4681 ggccggtatt ggcgggccga cttcagcagg tcctgtacga acgtgaggaa acggtcgggg
     4741 tgtccggtca ctaggccgag gtgcgtcgcg ttgcggcgta cccacacatt gaggttggtg
     4801 gcgacctgat gccggtcccc ggcgtggccc tggcgtacgc aacgggcaag gaggtacgcc
     4861 ttgatgaagt gggatgccgc caggggatcg tcacggtcgg tggagagttc gcgcagcatt
     4921 tcctgccact gggtgttgag ttgctcctcg ccgtccccga cgttggagag cagatggctc
     4981 ttgaggagat cgacggctgt gaggcgggcg ccgcggtcgt tcattgtctc gaacatgcgg
     5041 tagccgtggt tggggccggc cgctcggatg ccgaccagga cgacccgttt caacagccac
     5101 tccgtgaagt ggcggagttc ctcggcgtcc agttcctcga tcagctggta ttcgatctgc
     5161 tggttgcggg cccacaggtt gcggcgggag agggagtccc cggcgcccgt ctcgtacttg
     5221 cggccctcgc tgacggcccg caggacgggg tcgtggtcgg tgataccgac gcagaagctc
     5281 ttgccgtccg tggtgatgac acggttcagc tggtcgacgg tctgtgaatc ccccatctcc
     5341 ctggcccaca gacgcagttg caggaaaagc acatgcaggg tgacgaaccg ctgctggccg
     5401 tcgacgagga aacgacgatt cctcaccggt tcgtgataga cgaagggacc gaggaagtac
     5461 tgcggcgcgg tctgcggacg tcgtcggtag accgagtcgc ccgaccagtt gcgaaaggag
     5521 tcgcacaggt cccgcagcag ggtgcggact tcctcctcgc cccaggtgta gtcccgctgg
     5581 tagtagtcga tctcgtaggt cacatcacga aacagttcat tcaggttgaa gccctgagag
     5641 ctgatctcgt ccgccgccac gcaatcaccc ctcacctgcc gggcatatcc cgccatgctc
     5701 ccagcccggc accctttcgt acagactgtg tggggccggg cgggctgccc gcaccgcacc
     5761 ggtcctgccc gcccctcacc gaccctccgg caacggtccc ctcgcgaggc gttcccggaa
     5821 gccacgaaga tccacgtccc acgggcccga cccgcacacc gacggagcgc gcctgcgccg
     5881 actggcaccc ggcttcactc gcggccctgt ggtgacactc ggacgagtgg atgccggtgg
     5941 gccggtgggc gggtggccga acggcttgcc cgcagcggct acggatgtgt gaccaggtgt
     6001 tttggagcag tcggatgaac ggcgacgcgg ctcccgggac tgtggggcgg cggatcgccg
     6061 aaaagacaac cgcggtcggg gatgtttctc ttcctgcgag agccatttct gttcccgcct
     6121 cggtggattc gggggttggt cgtcttcgcc ggtttccgcg ggctcacttt tcttccccag
     6181 acagggcctc catgaagctt caccacatcg ctggcgcctc cctcgccgcc gccgctctcc
     6241 tggcctgcgg tacgcaggca caggctggca ccggcacccc ggccgaggct tccgtcatcg
     6301 aggggcgctc tctgtccgac cccgtgtcct gcggggtctc caccaccgcc acccaggcgg
     6361 ccgtcaccgc gtgcgcggca tcctgatccg cgtcgcatcg aacactcccc cggcccgtcg
     6421 ggccccgaga ggagacacat cgtgggccag aacgccgccg cctcgcagcc ccccgccgaa
     6481 caccacgcgc ccgagcagga cctgtccggg ttcgtcggta gccacttcat ctatacctac
     6541 gccaacggct ggcagtacga gtggtacgcg cgcaacgaga aggaatgcga ctaccgcatc
     6601 caccacggcc tggtgggcgg tcgctgggtg accaggcagg ccgtatccat cgttcggttc
     6661 tcggacggcc agtacaaggc cgactggcac gagcccaccg gtacctgtgt gagcctgctc
     6721 ttcgatctcc cgcgccgtct gttgcacggc actgtcttct tcccgcagtg gatcggcggg
     6781 gagggccggc atcccgagaa gacaatctgc taccagaacg aattcaccga cgacatgcac
     6841 cgattccgcg atgagggccc cgcctatccg tacgtggtca tcccggaatt cgcgaagatc
     6901 acttatacgg agcagcgcgg gcgcgacaac gacaccgtca tcgatgctgc tccaggacag
     6961 ctccccgacg gttacgcgga ccgcaccaac tgatccgagc aaccggcccg agcactgacc
     7021 tggccgagct ccggaccggc tcgatggccc tgcggggccc ctgtacccgg ccatcccccg
     7081 atgccccgcg gaacagagaa aaacgccctc gcggacggcg atatcgcgcc gatatgcgcc
     7141 tccgcgcatc ctgtgttgcc attgaccaca gcaggtcttc tctcaggagt gcgtacaacg
     7201 tcggcgtacg cggcaggtca gggcgcgtac gtggcacgcg ggtggcgggt cccggacgga
     7261 ccgccccctc cctgcgactc cgactccgcc gagcctcctc ccgcggccgc ggcggtgacc
     7321 gcgagacctc gtcccgcccg aaagccctca ccaaagctct caccggaagg ggcggcggag
     7381 ccatgccgac ccatgacgcg tcacgtgcac tgctcatttc gcgagaggcg ctggcactcg
     7441 cccacctcaa gacggcggca gaacccgtgg atatccgcgt cagtgcggag gtgctggaca
     7501 gggtggccgc ctgtcacacc ttcgcacgac gccacatcga tgcgggaaac gcggtgtacg
     7561 gggcgtccac gggcttcggg cccctcgtcg atttcagggg gagggacgag acccagcagc
     7621 aggccgagaa cgctctggcg catctgggtg ccggtcaggg gcccaacctc gatgcgcccc
     7681 tcgtacgggc cacgatgctg gcccgcctgt cggtcctgtg ccagggacgg tcgggagtgt
     7741 cgacgggcgt ggttcaagca ttggcggcgg cgctcggcag ctccttcgcc ccggccgtgc
     7801 cgtccaccgg ttcgctggga gccagcggtg acctgattcc cctctcgtat gtcgcccgcg
     7861 ccctcaaagg tgacggcgaa gcctacgcgg acggcacctt gatgcccgcg gcgcgagcac
     7921 tggcacattg cgggctcact ccgctcgaac tcgacgagag agaggccctg gcactcgtca
     7981 acggcacgtc actgacggct gccgccgtgg gcctcgcgac cttgtcagtc gcccgttcgc
     8041 tcaccgtctc cgttctcctc acggcgttgc tgtgcgacac actgggatgc tccacggagt
     8101 tcgcctcccc cgccctgctg aggctctacg cccacccgga cgccgaacgt gtgggcgcgg
     8161 gactgcgcgg acaactggaa ggcatcaggc cggatgacgg ccgttccctg caggagccgt
     8221 acagcgttcg ctgctcccct cagctgatcg gcgccgtggg gtcgaccatc gcctacgccg
     8281 accgcgtcac gcacgacagc ctgaacagcg tcacggacaa cccggtgttc ttcgtcgagg
     8341 acgaatcggt gatccacggc ggcaacttct tcgggcagtc cctcgccttc gcaggtgacc
     8401 tgctgtcggt gggctccgct caactgggca atctcgccga acggcagctg gacgcgttgg
     8461 tggacccgcg gcgcaacggt gggcggcctc ccatgctgtc gcccgcccct ggccagcagc
     8521 acgctctgca gggggtgcag ctcgccgcca cggcgatggt cgtggagatg cggcgcgaca
     8581 ccgtgccggc ctccgcgcag agcctgccga ccaactggca caaccaggat gtggtaccgc
     8641 tcgggaccca cgccgccctc accgccctcc ggcaagccac gtcactgcgc ctgctgcacg
     8701 gttccctcgc cgtcgcgctg cggcaggccg tgcacgtcgg cgaacgcgag ctcacggctc
     8761 cggcctgcgt ccggctctac gagcgactcg ctgatgtcgt ggaccaggtc gacaccgacc
     8821 ggccgctggg ccatgacgta cgtatggcgg ccgatctcct ggaccggatc gtggacgagg
     8881 acgacgggct cacctcgcga tgacccgtcg gcccggcacc cacttcacca acgatcgggg
     8941 gtgaaggaga agcgagatgg cagcacggac ccagagcgta cggtgtcacc aagtggccgc
     9001 tgccccacgt acgggacggg tggagaatct cctcctcacc ccgtccgggg agaacgacct
     9061 gcggtgcggc tcacctgccg cctgtgacca gccggctcgc gacgatctca tcctgctcag
     9121 gcaggccgcc accgggactc ctccggttcg gctgctggcg ccgggtgaac ccgacgcgcg
     9181 tatccgttac cggtgggcag tcggccacca cgcggctttc ggggtatggc ggctgctcgc
     9241 cgcacggttg cgcaccatcg ccgatacggc cagccccgcc ggaagcgcca tcaccgaggc
     9301 cgcgcggctg tacgacgtgt actccgtcct cttcctctac acgagcagtt gttcacccga
     9361 gcggtacgac gccaccgtcc gcgccgacat gatcgcctgc gaccctgcgt tcagcggcac
     9421 gtggagccgc gaccacgagg ccgtaccggc gttgatgcac aggatggacg aggcgcacga
     9481 cgccggtctg accagagaga tcaagcgcgc cgcgcggctc aacggccaga tacacatggc
     9541 ggtggcgaag aagcttgtcc ccgagggggt gtcactgctt cagcaggagg gtggtccacc
     9601 gcatcggccc gcatcggccg agggcgacct gtatgacgag ttctttcacg tgcgaaggac
     9661 cgcgacctgt cgttgcgcgt tcagcgctca gatcctccgc cgtctcacgc aggtggcgac
     9721 cgacatggca caccacggcc tgcactcctc cgacgatcct ctgccggggc tcggcgtgaa
     9781 ggcggcctcg ggtctgcggc gcatcgagtc cagtgctcac cagcaactgc ttcacctggc
     9841 cgccctgttg gcttcggaga cctgtcgggc ggacacggga cgccggggcc acgcggggtc
     9901 gtccttctcc acccacgtat gaggatccgt ccgcaagccg cccaccggcc cggtcgaggg
     9961 agcggcgcca tgaaccctca gcccaggcac cgccggtgcg tcagccgcgc acgacccgta
    10021 cgccggtggc tcccatcagt ccggccaggg gcaccttcgc cgaggtctgg ccgggatcga
    10081 ccgtgagccc gtcacggcgg atcaggtcga gcagccgttc ggcgaggggc atgaccatgt
    10141 gccgccccca acagcgttcc ccgacgtggc cgaagggcag gtagccgggg tgggtgtcgg
    10201 ggtcggccgt cgcccaccgc tcgggcctga agacgtccgg gtcctcccag tggctcgggt
    10261 cccggtgact cagcagcggg agcatgagca cgtcgtcagc gaggccgatc cggtcgtcca
    10321 gggcggtgaa ctccgcgtcg gcgacccgca gcaggttcca ggagacgggc agcagccgca
    10381 gactctccag caggatgtgc tcgttcgggg tgtcggtggc gaagggtgct cccagccaca
    10441 gcgcgttggt gacgagcgcg gagacggtga agcacacggg ggcggcggcc cgccggtaca
    10501 tgcccatggc gtgcctgcgg ccctcgtatc cggtggccga ggacacgaga ccggcgagct
    10561 ccgacagagg gggccgaccc gcgggcggta cggggagggt cgcgccggcc gcgatgacgc
    10621 tccaggtgat tttcggcgtg agttccaggg tgcggtccac gagcacgcgc agccggtagg
    10681 ggtcggcgcc gaagaccagg tcgcgaagat agcgatggcc cacctgtggc cattccccgg
    10741 tgaggtccac cggggtgtcc gtcggcgttt tcagggcggt ccgcacatcc tgcccgacgg
    10801 cgcgcatgac ggcggaggcc tcggggcggc ctattttcct cccctgaagc ggtttgaatg
    10861 tcggtcgctc gaattcattc gcgggccggg cggcgagtac cgcgttcatg aggtcggcgc
    10921 ctgcgattcc cacggtgctt tcgtccagcc ggaaagcttc ggcgcccctg taactccgca
    10981 aaaggtgttc gatacgcggg gcgaacaccg tttcccggcg gagccgctct gtaccggtcg
    11041 tgctcatcag cgcctcccag ggcccgaagg atcgaagtgg cgggcggtgg gccgacgtac
    11101 tgaaagcacg tcggcccacc gcccggttca ccggcacgat tcgccggtgg ccccgtcagt
    11161 accagatgta ccaggcatac gccttgaggc ttttttcggg cgcgatcttc ttcttcaggg
    11221 agctgaggag cttcatggcc gtacctccga tgcgaatgaa ccgctgaatc cttttcgtgc
    11281 gcagcagggg tgcgtgatct caaccccggc cgccgaaagt gaatccggag gctcccgcga
    11341 gtgtcaacat gacgcaactc accgagagtt cgggattagg cggggccgga ttggtgaatc
    11401 acgtcgaacc gcccaaatgc ctcgttcctg cgggccggtt gcccatggat cagccgaggt
    11461 actccggtga ccacggaata ctccggtaaa gcctgcgtcg acaacacccc aagacgtatg
    11521 gcggcaggac acgcccttcc gtcgcgggtg gtttcagagt tggccgacat acaggagccg
    11581 gtcgggagag cccgcgccgg gtgagacgac ggcgccggtg gtggccctgt cggtgagaag
    11641 gcgggtgatg gtcgcgggag tggcgccggg gtcctgctcc agaaggagag cggcgccgcc
    11701 tgccgcgtgg gggctggcca tggaggtgcc gctgagggag cgggagccgc cgccgagccc
    11761 ggcggagacg atgtcgacac ccggcgcgta cagcttcaga cacggcccgt agttgctgaa
    11821 gtcggcctgg cggtcctgcc ggtcggtggc cccgacggcc agagccttct gcgcgcgggc
    11881 gggagaagcg gtacaggcgt ccacggcgtc gttgccggcc gcgacgacgg ggagggcccc
    11941 ggcgtccgcg acggcgttgg tggcggtgtc cagcgtggcg gaagccgaat cgccgatgga
    12001 ggcgttcatg acggcaggcc gcttgaggtg ggtggcgatc cagtcgaggc cggcgagggt
    12061 ctgggccgtg gtgccgctgc cggtgcagtc cagtacgcgt acggcgacga gggagacacc
    12121 cttggccaca ccgtaggtct tcccgccgac cgttcccgcg acatgagtgc cgtgggagtg
    12181 gcagtcctgg ccgtcgcggc cgtctccgat cgcgtcgaat cccacggtgg cgcggccctc
    12241 gaactcggcg tggccggtct cgatcccggt gtccaggacg tacgcggtca caccacgtcc
    12301 tgtcccggtc acggcgaaac ggccgtcgag ggggaggcgg cgctggtcga tacggtccag
    12361 cccccagctc cccgcggcca cctcactagc ggccgtcggt gtccttccgc gcccgccggc
    12421 accgtccgcc ggtgcgctgt tgagggtgac ccggccgttc tcctcgatgg ctgatacggc
    12481 gggttgagtg cgggccagtc gcagctggag tggggtgagg gcggcggcga acccgtgcag
    12541 ggccgtgctg taggtgaaca gcggtctgat gtccagtttc cgcatgagcg ccttcggcgc
    12601 gaacgagggc ttgaccgtga cgatgtactc acccgccacc gccttcgccg cgtgctgcgt
    12661 gaccacggcg tccgggccgg gagcggccac cgcgcccgat ccgcccaaca tcggtagcac
    12721 catcagaaga acagacgccg ccccgcgcat acttacccgc atgcagactc catcacagaa
    12781 ggggaatgaa caaccggaag gcgcggctca cgacgaaccg cagccgtacc cttcttttcg
    12841 gcgggccgac gccccggctt gagtggcgcg gagcccgagc cacgccgggc cttcccctcg
    12901 cggtctgttg ccgtgcgggc ggcgacggta cgtgggggtc ctcgcgcccc cgtgcgattc
    12961 ccgctgagcc ccgccccacg gcaaccgatg gcgatgagcc ttgggttgac gaacaggacc
    13021 gcacgggaaa agatcatcag gctcgaaatt gacaacgttg tcgtacgaga ggatcgcgat
    13081 gagagcgttg agacgcgcgt tcaccgcctt gttggtcgcc accaccgtgg ccacggcttc
    13141 cgccgcgtcc ggcgtggcca acgccgcgga gcgagggccg aagttcgccc atgaccccac
    13201 gacgctcgtc gacacgtcga tcggcaacaa cggggacggg accaccttcc ccggcgccgc
    13261 cgccccgttc ggcatggtgc agctgagccc tgacacgcag ctgaacaagt acgcctccta
    13321 cgactacgcg caggacacca tcctcggctt cagccacacc cacctctcgg gtgtcggctg
    13381 tcagacgatg ggcaacttcc ggttcatgcc gaccaccggt gcggtcacgt cgtccgaccc
    13441 tgcgcggtac gctgcgaagt tcagccatga caacgagacc cgcgcgcccg gttactacgg
    13501 cgtgaagttc gacaacggca tccgggccga actctcggcg acgcggcgca ccggtcagca
    13561 ccgctacacc taccccgccg ggtcgggctc gcagaacgtc ctcatcgaga ccggtgagag
    13621 caacggcagc acgtacgcag gggacatcaa ggtggtcggc gacgacaccg tggagggctg
    13681 gctccaaggc ggcaacttct gtggtgagac cggtaaggag cgttaccgga tcttcttcag
    13741 cgccacattc gaccggaagt tctcccggtt cggcacctgg tccgacgaca ccctcacccc
    13801 gaaccagcgc gaggcgtcgc gcggcaccaa gcgtgcgggc gcctggctga ccttcgactc
    13861 cgccaagggc gaccaggtcg gtacttcggt gggtctctcc tacacgtccg tcgacggcgc
    13921 ccgcctcaac cgcaaggccg agcagccgaa gtcgttcgac aaggcccgcg gttccgcgca
    13981 cgacgcctgg aaggacgagc tgaaccgcat gcgcgtggcc ggcggcacca ccgccgacca
    14041 gcgcacctac tacagcgcgc tctaccactc gctgctgcac ccgtcgatcg ggtcggacgt
    14101 cgacaaccgc taccgcggct tcgacgacaa ggtgcaccgc gcggactcca cgtactacca
    14161 gatgttctcg ctctgggaca cctaccgctc gcagaaccag ttggtggccc tgctcaaccc
    14221 ggacgagacg gccgacatgg ccaagtccgt cctgcacgtc taccaggacg gcggctggct
    14281 gccacgctgg gggctcggca acggcgagac caatgtgatg agcggtgacc ccgtcacccc
    14341 gtggatcgtc gacatctaca accggggact cctcgacaag cgcacctcgc gtcagctctt
    14401 cgacgcactg tggaagaacg ccaacgaggt tccctccgat cagtccctct tccgcggccg
    14461 tgacggcaac ccgacctatc ttgagaacgg ctgggtcggc tatcagaacc tgcccggtta
    14521 cacgtacggc gacagccgtc aggccggctc ggccacgctt gagtacgcgc tcgccgactg
    14581 tgcgctgtcc accatggccg acggcctcgg ccaccggggc aaggcgtcga cgctcgccgc
    14641 gcgctgcgac aacttcgcca agctctggga ccccggcgtc acctcacagg gcttctcggg
    14701 tttccccgtg acgaagaacg cggacggcag cgtcgccggc gaccccgatc ccacccaggc
    14761 gggcgcgttc cacgagggca ccgcctggca gtaccagtgg ctggcccagc aggacccgaa
    14821 gaccctcttc gggctgatgg gcggccgggc cagggccgag cagcggctcg acaagttctt
    14881 cgacatgccg accgttctca ccgacccggc gaaggcggct tcggagtcgt gggtgaccgg
    14941 cgcgtacgac taccacaaca atttcgcctt caaccccaac aacgagcccg acttccacac
    15001 gccgtggatg tacgcgtgga ccggcgcgcc gtggaagacc tcagccacgc tgcgggccat
    15061 gcggacactc ttcaccgatg acgcgtacgg catgcccggc aatgacgacc tcggcgccac
    15121 ctcctcgttg ctggtcttcg ccatggccgg cgtgttcgag gcccagccgg gggcggccga
    15181 ctacctcgtc accgcgccca tgttcgagaa ggtcgagatc aggcccgagc acggacggaa
    15241 gatcagcgtc gaggctccgg gcgccagggc gtcgaagctc cagtacgtgt cctccgtgca
    15301 cacgggcaag ggtgagctgc gccagagctg gctctcccac aaggacctgc tccgctccgg
    15361 cacgatcaag atcaagctct ccgccaagcc gtcgaagtgg ggcgtcggct ccgccccgcc
    15421 cgccatgggg gcccggaact gaggttgtgg ggtgcggccc tccgcacccg ctgacgacgt
    15481 gtcggcactg tggcggccgg tgcccaggcg tcgtccgcac gcaccatgga gacgctgaga
    15541 cgtgcgggaa gggggtgtgg ctgtctcggc cacaccccct tccactgctc actccgtccg
    15601 gttcagcagg ggccgtcgtc ctgccaggca ccccactcgc cggtggtgcc cggcttctcg
    15661 cccgtggtcc accacttcgc ctcccaggcg tggccgtcct gggtcacccg gtcgcccttg
    15721 acgtagaccg ccgaggcgct ccaggcggtc gtcgggcagg tgccgccggg gtcggtgggc
    15781 ggatcggtcg gcgggtccgt gggcgggtcc gtgggcgggg tcgctccggc gaacctcact
    15841 gagaacttgg cgaagtccca ggccgactgg ggcacactgc tgcaggtgcc tgaggtacgg
    15901 ccgttgttgt cgggcgggtc gcactgcctg tcacggttga gcgaccagaa ggtgaagcgg
    15961 tccatgccgt gcgaggtggc gtagtcgagg acggtctgga agtcggtctg gctgaagaac
    16021 tccccggtgt cgctgcggcc gttcatcccc gagaagccct cgtgagcgta ggcggtggcc
    16081 tcgctccagc cgaaggtcga gcgcagcacg ccgttgaagg cggtgagcgc cgcggtctgg
    16141 gcggccgctc cgctgaagcc gccgtcaaag ggcatgatgg agaagttgtt gggcgtgagg
    16201 ccctgtgcct tggcctcgtt gagcatctgc ttgccgaacc agccggtccc gtcggcggtt
    16261 ccggctgtgg tcactgagac gtacagcccc gggttcttct gctggaggat cttggcggcg
    16321 ccgatctcgc gggcgatcgc tgccgtgttc tcgtactccg gttcctccag gtcgaaatcg
    16381 atcgccttga gcccgtactt gtcgatgacc tgctggtacg cggcggccgt ggaggcggcg
    16441 tcggagcagg cctggccgag tttggttccg ccgtagccgc cgatggagac ggagacgtcc
    16501 ccgcccttgg cgcggatcgc gctgatgacg gagccgacgg cggtgtccga ggagacgggc
    16561 gccgtcccgt cccaggtcgg gctgcatccg ccgccattgg gggcgaggat gaaggcgagt
    16621 tggaaggcct tcagcccgga ggcatccatg atcgcggtgg cgctcggcgg gtcgttgtcg
    16681 agcggcatga ggtacggggc ggccgcgtac caacggttgc tcagcgcggc cgtggagctt
    16741 tcggccccgg acgcggagga gggaaggagt gcggtgagcc cggctgccgc gacggccgtg
    16801 gacgcggcgg cgcagagcag ggcacgaaga cgtctcatga ggtctcccga ggaggtgaag
    16861 aggcgcctca ctttcgcgcc gttgtagcgc acacgtcaac ggattggact agaccaagac
    16921 gaactcttga cctggatcag gaggcagcgt ccgtaccggg gatcaggtct cgcggagtgg
    16981 ctcggtgcgg gcaaccccgg tcggcctcgc gggtcgcctc ttcgagcacc gcgtagtacg
    17041 cggcggggcg gcgcacgaag tccgccaggg tggcgaagcc ttcggggtcg gggtcggcgc
    17101 cgcgcagcag gtccaccgag gactgcgcga agccccgggc gaggtggtcc tgtccggccg
    17161 gggcgatgac acggtgcagg gtacgtgcgg cggtgcgttc ctcggcctgc tccaggacgt
    17221 cgggtgctcc gcgtcgaggt gtacagcggc ctggtcgcag gctcgggcgg tggtgtgcgc
    17281 gagtgcggtg agtacctctt ccgtcacctg tcggccgagt cctggcagcc gccccggggt
    17341 ccgggccgcg gcttcgcgta cggcgtccat cgtgtccagt ccctcatgcc ggagtccgct
    17401 ggcgtgcggc agggttcgga cggggaggcg gtcgagtgcg gcgcgtaccc gcgcctcgtg
    17461 gagcggggtc caggccacca ccacggcctc ggtggcgcgg aggtggtccg cacggacccg
    17521 cgcggcgctc tctcgcagtt ggttcgcctg ccgtacgatc gcacggctcg caccgccgtc
    17581 ccccggccgc actgccgtca ctcccccggc gctgagcccc gtgagggtca ccagcagccc
    17641 aggcagcacc aggacatcgc cgatgtcgac gagtgtgccg accgtaccct cgtcctggtc
    17701 cagggagtac aggtcaccct ggtgggcgcc ctccgtgagc atccagccgc tgtcccagcc
    17761 gtggccggtg tccgggtcgt agcgtggggc tttgaggagg gcgtcgcggt cggggtcgca
    17821 ctggtgggcg ccctcgcggt cacccggccg gggcaggccg atgcccgccc cggctgccgc
    17881 tcgggtgtcg cgaccctcgg cggaaaggtt gagcccttag ttcggcggaa ggttgacccc
    17941 ttagcatgtt cgaccaggaa gacacccagg ctgtcgagga gcacggtgcc gccctgttcc
    18001 gcgaacggga cctggccgcc gtcgggggtc ggctcggggt agcccggccc ggcccccgtc
    18061 gtgctccacc gggctgaatc gatactccgt tggccgaccg ggacaggacc ggctccatcg
    18121 cgacggatgc gcacggagtg ccgggaggtg accagagcgc gccggacggg ctcgtggtgc
    18181 gccctgacgt cggtccgggc gtcgccggca tccgggcacc cctcgcgtcg agtgcgccac
    18241 ccgcgaccgg tggcggtccc aacggccggg cgggggcggg gtcctggcgt gattcgtcgg
    18301 acaccgacac cggagacgtt tactgtacgg atacgaggtg tcactcaccg cgtgtccgtg
    18361 atttcgagga ggactcccta tgtcatcttg cgaccccggc cacgctgccg tcggggatgt
    18421 gggcgcagcg ctcatggtga tggactcccg gctcaaagcc gtctacgacg ggaagaccgc
    18481 gaccgacccc gagcaggagg cgctgatcga gcagatcacc gcttcactgg gtcccaaggg
    18541 agccgacgat gtgctggacg gtgcgtgcac cctcatcttc atgtacatga aatggctgcg
    18601 cgcggcctac gaggaccagg agaaggacgt catcgagtat gtggtgacca gcctggtggc
    18661 ctccatgcgc gtgatgacca ggagcgttcc ccctgaggtc atccccacca tgaccggtct
    18721 ggtcgtcgcc gcgggcaccg gtctgagccc caatctctgg cgcaagcagt acggatactg
    18781 gactcgggcc gagatgaccc ccttggaagt caccgtcgtc ctgctggctg agcacatcaa
    18841 ccgcctcacc ggcgaccgtg actacgcgac tcgcctgatc accgacgcgc tggccggtac
    18901 ggacatcgac tgagctcccg acccgtcgca cgctcctggg agtgcgcccc ctgcccccac
    18961 gattccggcc ccacccggac gatgtccagg cagacgtgcg aagaccgtcc gggcaggcga
    19021 ccacgggcac agactgcctg cgtggtccgt ctgttcgctt catgggcccc ggtagtacgg
    19081 cagtgttgac cgcagcggta cgagggcggg atcgtggatc ctggagcacg tgcggaacgg
    19141 acctcggcgt ttcgtcggct taccgatggc tcctgctgct gccgcggacc atcggtaccg
    19201 tgtcggctgc ggcggcccgg cgagaacacg gaggcacgat gggcacctcg gccggtcccc
    19261 cggagcccgc ccccgtgatc gagcgggctg aggcgacccg cctctggggc agggaagtga
    19321 aggaccgggc ccgccggatg cggatacggg cgctggagct gcggcagcgc tcggagcaga
    19381 tgcgctccgc cacagcggga tcactctccc ggtgcaggca ggtgaacgcg gcgttccgaa
    19441 ccgcccgcgc cgccgcttcc gcgcacggtg gtggcgctcc cgggcggggt tgtggcgctc
    19501 ccgcggcgca gtgggcggcc ctggtcgctt tcgtacacgc gcgtctggac gaggaggcga
    19561 ccgccgcgga cctccaccac gaggccccat gcccgacact gggcgggccg gggcgcgacg
    19621 gggtgacggt gtgcggctgc cgcgccccgg ctcacgtgcg ctacgacgtc tcggtccgtc
    19681 gcggaatcgt ccgcgacatc gagagggccc tacgtgccgc cgaccactcc acaccgaact
    19741 ggccgtacag cgagctgtcc gccctggtga ggctgaagac gtccgctctg ccccacgagc
    19801 tgcacaagca atggcgggaa gcctggcggc cctgacacca ggggtgaccg ggctcctctt
    19861 cgcggccgct ggtccgcgtc ggggcggagg gcgcgttgag gggcgcacgc gccttgggcg
    19921 atgactcgtt cgacacttcg cgcgccctgc cgtggccgcg ggagcgccgc gatgatgagc
    19981 cgaccgcacg tgaccccagg actgcgttca tctgttcctc cgacaggcgg acatacggta
    20041 gaggagtcca tgttgttgct tctcatctct cctgacagcg tcgaggaggc cctcgactgc
    20101 gtgaaagcag cggagcacct tgacatcgtc gacgtgaaga aacccgatga gggctccctc
    20161 ggtgcgaact tcccgtgggt catcagggag atccgtgacg cggttccctc ggacaagccg
    20221 gtgtcggcca cggtgggaga cgtaccgtac aagcccggca cggtggcgca ggcggcgctc
    20281 ggtgcggctg tctccggtgc cacgtacatc aaggtcgggc tgtacggctg cacgacgccg
    20341 gaacaggccg tcgacgtcat gcgtggggtc gtccgggcgg tgaaggagta ccggcaggac
    20401 gctttcgtcg tcgcctctgg atacgccgac gcccaccgga tcggctgcgt caatccgctg
    20461 gcgctgcccg acatcgcccg tcgctccggc tgcgacgcgg ccatgctcga caccgcgatc
    20521 aaggacggga cacggctctt cgaccacctt ccgcccgacg tctgcgggga gttcgtccgg
    20581 ctggcccacg aggccgaact cctcgccgcg ctcgccggca gcgtccaggt gggggatctc
    20641 ggtgcgctga cccgcatcgg cacggacatc gtgggggtgc gcggggcggt ctgcgaaggg
    20701 ggcgaccgcg accgcggcag tattcagccg gggctcgtgg ccgccttccg ggcggagatg
    20761 gaccggcacg gccgggagca cgctgccgcc gtcgccgccg cgagctgacc gccggtatgc
    20821 cgatacctca gcgcggaccc ctgcccggaa cccgcggcga gtacttccgc gtggtcgatc
    20881 ccgcgacggg tgagaccttc gacgaggccc ctgatcagcg tccggaggag ttggacgccg
    20941 tcgtcggccg ggcgcatgag tcgtggcggg gctggcgggc cgatcccgcc gcccgcaccg
    21001 gcgcgttgct cgcggcggcc gacgccgtgg aggcggccgg agccgacctc gctcccctgc
    21061 tcacccgtga acagggcaaa cctctggccg agtcgtacgc ggaggtcgcc cgcacggcgg
    21121 cccgcctccg ctacttcgcc gagctgacgg cgccggagcc ccagccgatc gcggacggcc
    21181 ggccgctgcg cagcgaggtc cgctggcggt ctctcgggcc ggtggccgcg atcgtc
//
