LOCUS       NZ_CP045178            20678 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 chromosome, complete genome.
ACCESSION   NZ_CP045178
VERSION     NZ_CP045178.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615214)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3698
            Orig. end                         :: 24376
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045178.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20678
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20678
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20678
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10678](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10678)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     misc_feature    complement(761..776)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.34"
                     /tool="antismash"
     misc_feature    1160..1169
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            1412..2245
                     /locus_tag="GBW32_RS00100"
                     /old_locus_tag="GBW32_00100"
     CDS             1412..2245
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129558.1"
                     /locus_tag="GBW32_RS00100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00100"
                     /product="TnsA-like heteromeric transposase endonuclease
                     subunit"
                     /protein_id="WP_227024937.1"
                     /transl_table=11
                     /translation="MEQQAVKGRGEAVIETAFATAAGQVTQLSWDQAAATVRFEDLAPV
                     SAFPVVPGRRWGPGWWWSATTGRHVVHGSAAMRTQLMVLDRDPDVTGLSARPVRLLWRD
                     MSDGRVRSWVPQLFARHADGTGLLADCPSSPTTGGDRAQRARMVLEAACARVGWAYRRL
                     EPPPTVVAANLRWLAGYRHPRNQGPPGIGAALAEVFAAPRPLADGAAAVGDPLQVLPAV
                     YHALWCGHLTTSLDEPLHEEALICADAERSGEGVEKRLGAGGGQGRGECGGRHTR"
     gene            2226..4382
                     /locus_tag="GBW32_RS00105"
                     /old_locus_tag="GBW32_00105"
     CDS             2226..4382
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010069771.1"
                     /locus_tag="GBW32_RS00105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00105"
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="WP_179120366.1"
                     /transl_table=11
                     /translation="MGGTRGKSGRPVVEVGAHVTYRGQTWQVAALQGPRVYLVQEAGTE
                     VSLLLGRLFADPDFEVVGARAPDTVPQWGLFEAVPVAAQQRALAWLPHIREVETGWPHP
                     EGGRDGHRMRPEYDPERWTLAQREAAKAKELTALGFARVTSTTVQRMRHAYRKQGLWGL
                     VDKRAVPARGRHPTGYADERVVAAVLEGLRRQRGRSKGTVKGLQVLVGQILQDTHGRGV
                     VEMPSRSTFYRLVSVLADPADRPGLPARTGSTPARASSGPPVVLRPGEQVQIDTTRLDI
                     MAVLEDGSLGRPELTIAVDVATRSILAAVLRPHSTKAVDAALLLAEMAVPHPARPAWPA
                     ALYLSHAQVPYQRMLSLDERLEGAAARPVVVPETIVVDRGKIYLSQGFVAACETLGVSV
                     QPAPPRRPQAKGVVERTFGSVNDLFCQHVAGHTGSNPQRRGFATAAEARWTIPQLQDFL
                     DEWITCGWQNRPHDGLRHPVLPKTALTPNQMWAALITISGYVPVPLTGADYLELLPVRW
                     QPITERGIRLDYRTYNHDILDPHRSQHSPVTARDGKWEVHHNPHDARQIWVRLTDGHLH
                     EIPWIHRDHVHQPFNNTIWRHVQTEVEQRGDREQHEADLAGALDQLLRRTRHPAETEQK
                     TRRRRAPRSDTAAQLPDLPGQRRPLDAETAPGPAPDWSESLDDLISVDTPAQTGTSEPE
                     GAGGLPGAAGGYGLWDAEAEAEQW"
     gene            4496..5469
                     /locus_tag="GBW32_RS00110"
                     /old_locus_tag="GBW32_00110"
                     /pseudo=""
     CDS             4496..5469
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550093.1"
                     /locus_tag="GBW32_RS00110"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00110"
                     /product="TniB family NTP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTWDGFQAFATTPVAAPPPPARRPAVSRNGWPTTRGSSPCAPPP
                     STPSPRTCAP"
     gene            5466..6422
                     /locus_tag="GBW32_RS00115"
                     /old_locus_tag="GBW32_00115"
     CDS             5466..6422
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00115"
                     /product="TniQ family protein"
                     /protein_id="WP_227024938.1"
                     /transl_table=11
                     /translation="MSPTDAAARAAAGAGTAQRSVVTTEPWHRIPFAAPGVFRVRPLPR
                     EATASYAQRLADTYQLTLPQLLDGSGITLHRHGTPPTAELILTPGAARRLAVLARTALP
                     QLTCALPHLPLSDTAHDTEAAANWKRLDAGQQPVRACTLCTRHRSHNATDTAWIHPPPH
                     QLVCPRHHQAAPDPRLTSTVHTRDVPELAAAHHAHQRLLRHPRAATAWITARAITTRWY
                     DHQQHLTHRWHTRLTQLRATNPHVTTTGSASPALLTRGLITYPETVALARVLTTLPSGH
                     HHTTNGALTLIARRLALAHLAPSANDPLRVFLTHTRH"
     gene            complement(6811..7230)
                     /locus_tag="GBW32_RS00120"
                     /old_locus_tag="GBW32_00120"
     CDS             complement(6811..7230)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00120"
                     /product="hypothetical protein"
                     /protein_id="WP_143621617.1"
                     /transl_table=11
                     /translation="MIHTIGHRVSRGFFVGAASAALLVGGGIGATATAHAPAASAAHET
                     TLAASNNLQVKPSKIAVHKNVAYTGSTPGLSTGTEVQLQQLIGKKWFDVNGKTAKTRAD
                     GTYGPIYDSFMYKGEKVMRTIAGGVPSNSVNILVS"
     misc_feature    complement(7216..7230)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.89"
                     /tool="antismash"
     gene            complement(8004..8747)
                     /locus_tag="GBW32_RS00125"
                     /old_locus_tag="GBW32_00125"
     CDS             complement(8004..8747)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.5"
                     /locus_tag="GBW32_RS00125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00125"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_077974357.1"
                     /transl_table=11
                     /translation="MPGHAFDQAAELYGASEAGLPFRRHIEIPSVLAALGDLRGKRVVD
                     LGCGAGLYARLVARGGASVVAGLDQSPGMIAHAHQRDRREASGITYLLHDISQPPPLPP
                     QSFDAVVCVYVLPYASTQRQLTDICRTARTLLAPGGRFVTATLNPDFATAHDYYRPYGF
                     TLTPGDHESPEPPEGHPVELNTWIDANTFTATARRWSRHAHRQALHDAGFTEVTWHAPT
                     LHDTPTDTHWTDYLTSPHTLIVNAR"
     misc_feature    8728..8743
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.5"
                     /tool="antismash"
     gene            complement(8781..10004)
                     /locus_tag="GBW32_RS00130"
                     /old_locus_tag="GBW32_00130"
     CDS             complement(8781..10004)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.5"
                     /locus_tag="GBW32_RS00130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00130"
                     /product="cytochrome P450"
                     /protein_id="WP_077974355.1"
                     /sec_met_domain="p450 (E-value: 4e-16, bitscore: 57.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVPDQSNPRIEQDLPLPYPFPLGDLGKLPPLIQWAQKSRPVCPV
                     LMPSGSRVWMLTRKNDIAAVFADPRFSRNLLAPGSPRISGDDVTAVGRGLFNLDPPDHT
                     RVKQVISPYFTRRATQTYLPLICERAHALLDTMAAGPNPTDLVTAYAARLQLTVMRGVL
                     GVPPELYDTYEQLFPTGASISAGPEATGNETQEIQRFVAEVIAARRRGPLLDDPLGALL
                     RAVDAGVISEEEMAGTAFVLFFTGSDAVVAPTTTGSMILMLHRKQLRECLDDPELWPRA
                     AEEVLRYYHNGVLGFPLVATEDVELHGQVVRRGEAVVASMQAATWDPEHVKNPEKFNIH
                     RSADAAATFGAGPHYCLGSQFARGYLHAALRALFERFPTLYLAVDEHHVPWRNDVIFVR
                     PVCLPVAW"
     misc_feature    complement(9366..9368)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10001..10678)
                     /locus_tag="GBW32_RS00135"
                     /old_locus_tag="GBW32_00135"
     CDS             complement(10001..10678)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF028025.5"
                     /locus_tag="GBW32_RS00135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00135"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_077974352.1"
                     /sec_met_domain="CDPS (E-value: 6.9e-50, bitscore: 168.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTLSPLPGGETFFPDRCGHALIGLSPWNGRYSRRYIEALVTWAH
                     SRFPRIDVFTPGYEAAHTLVAAGFPVGEAVHRARRANTQLRNPALRALRNAGVTEPQTH
                     VHTWTQLHARPAYTGARHRVQRAYASDPVVRRACRDTAREAVRGAGQREPDESAIDLAV
                     SYALAELPLVTEGPDIFGVPSSAFLYHRDMALIRPLISGESKSLVPHAGQRYAIATWEA
                     ETA"
     gene            complement(11038..11589)
                     /locus_tag="GBW32_RS00140"
                     /old_locus_tag="GBW32_00140"
     CDS             complement(11038..11589)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009999225.1"
                     /locus_tag="GBW32_RS00140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00140"
                     /product="EF-hand domain-containing protein"
                     /protein_id="WP_077974349.1"
                     /transl_table=11
                     /translation="MTSAVKSQKFNTLFDWFDQDRDGQLTQGDMQAMAGLFAALAQGER
                     ESATAMRDAFERWWQLLLTHGDTDGDGRVSRDEFIAAMEANVTSPENFGSAVLAIADAT
                     MQALDTDGDGVLSREEYVSMASRLSVSPVNSAAGFQKLDRDGDGVISHEEYRTALSEFF
                     LSADLDAPGNWLLGPIDSAA"
     gene            complement(11796..12029)
                     /locus_tag="GBW32_RS37600"
     CDS             complement(11796..12029)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS37600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_405517597.1"
                     /transl_table=11
                     /translation="MPRCHLASLKCSSTRRPPTAPGRGVARQPRLLRQYLPKGADLRPF
                     SQADLDTFAHELNHRSRKAHGYRPRPRSTLTC"
     gene            complement(12115..13308)
                     /locus_tag="GBW32_RS00150"
                     /old_locus_tag="GBW32_00150"
     CDS             complement(12115..13308)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714885.1"
                     /locus_tag="GBW32_RS00150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00150"
                     /product="aromatic acid exporter family protein"
                     /protein_id="WP_077974346.1"
                     /transl_table=11
                     /translation="MVGGARAGQGTQGRFARTAQWWAWALRYAGSERHALILIGKSTLA
                     ATIAWMISFYALDAQSPAFAPFSAVTIMYVTVYQSVTQSLRYVGAVTAGVAVQAVLGFL
                     TGSDLLTFALVAVITLSIGRSRVLGAQGPQVATAAFFAFSTYTSATDDMTRVSQLGQIV
                     LLVLIGCAVGTSVNIIIVPPMRYRSAEYSIRALARALYELTCDMPPVLRAGTLDADTTT
                     RWREHAARTGNLIAQARVGLNTAKESVHYNPRRLLRRNRGRLGFQRFESVLDALERTLA
                     QLASLTRSLDQWRKEENDYRYLHFLDRYAAFLEAVSEIALALSTLDETTLPQQTERLAG
                     LADGAQERRHELVDEAKAQALPLADPTRPYGVLVIEAARLMEEVQYTSDTLHKVADA"
     gene            complement(13738..14337)
                     /locus_tag="GBW32_RS00155"
                     /old_locus_tag="GBW32_00155"
     CDS             complement(13738..14337)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00155"
                     /product="hypothetical protein"
                     /protein_id="WP_152330717.1"
                     /transl_table=11
                     /translation="MLPTNGEPGPQAEQHHKPRQESRREGHRQQGPVAGAQRIIDGYNS
                     TPGPERVLLGLSTSFAITIATSRTINYVRERRRALPRIRGLTRLLMEIPLKNTVRVHHF
                     LPGIAIGFAVGCTALLVRPGRRERLLSLPLGVGFALITDEVRLLVGLNDPYWGRGERFA
                     HAQGAVATLAALGIGADVVRRGHPEPHRSVRGADRC"
     gene            14419..14577
                     /locus_tag="GBW32_RS35455"
     CDS             14419..14577
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS35455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_179120285.1"
                     /transl_table=11
                     /translation="MRPFSPATAAEVGEALNSRHTRMEQPRSPRTSTPTSIERFIEDEF
                     APRYRAA"
     misc_feature    14688..14701
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 19.91"
                     /tool="antismash"
     gene            14746..15879
                     /locus_tag="GBW32_RS00160"
                     /old_locus_tag="GBW32_00160"
     CDS             14746..15879
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062: glycosyltransferase, MGT family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908512.1"
                     /locus_tag="GBW32_RS00160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00160"
                     /product="glycosyltransferase"
                     /protein_id="WP_077972266.1"
                     /sec_met_domain="MGT (E-value: 3.3e-63, bitscore: 212.9,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLFSATPAFGHILPLVPLMQAAVSDGHAVGLLSSAEFRAAVGE
                     ELPPEVEFLDAGVMPAVFSEEAGRRTGADVFHPTPSTIGEIFGGARVDLAVEESIYQAT
                     DWAPDLMVAEPFDAVGPLVAARLGVGWHMAGIGPALPSVITDEVERVASTRYERLRLRP
                     VAASSYIDPCPRQLQDPEWSPAVQVRAVRPQAHRRPKDVALDLSGFGEPGKPTVLVTLG
                     TIFSDPETLAAAVAAVADTGVNVIATLGSSLRHSTASQATPADSANVRYVPFVPLDQLL
                     ERVDLVVGAGGVGTVVGTLAHGLPMVLWPQGADQPINAARAAASGASVTVDSAEGISPA
                     VADVLRNGTYRDRAQEIATGIAKLPLAATVISEITHP"
     misc_feature    complement(14747..14761)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.8"
                     /tool="antismash"
     misc_feature    complement(16193..16207)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.54"
                     /tool="antismash"
     gene            16341..17220
                     /gene="rfbA"
                     /locus_tag="GBW32_RS00165"
                     /old_locus_tag="GBW32_00165"
                     /pseudo=""
     CDS             16341..17220
                     /GO_function="GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="rfbA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982385.1"
                     /locus_tag="GBW32_RS00165"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00165"
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /pseudo=""
                     /sec_met_domain="NTP_transf_3 (E-value: 1.8e-10, bitscore:
                     40.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGIILAGGSATRLYPLTGALSKQQLPVYDKPMIYYPLSVLMLAG
                     IQDVLVISSHQHVETFQFMLGDGSRIGIHIDYAVQDEPRGVADAFRVGDKHVGNDRVAL
                     VLGDNVFHGPGFTTVLRRGMQRLDGCELFGYPSRTLRRRRNRRAGALVVAPGETAISPF
                     RPRGHRTVLLRQRCDRTGEGTQAFRARRTGDNRHQPRLFDARPGAADRARSRFRLAGPG
                     DPGLPAPGRPIRPASGAASRRTHRLYRGDRLAYGFHRC"
     misc_feature    17130..17132
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            17259..18230
                     /gene="rfbB"
                     /locus_tag="GBW32_RS00170"
                     /old_locus_tag="GBW32_00170"
     CDS             17259..18230
                     /EC_number="4.2.1.46"
                     /GO_function="GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (4-183). E-value: 3.2e-20.
                     Score: 64.2. Matches aSDomain:
                     nrpspksdomains_GBW32_RS00170_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072808.1"
                     /locus_tag="GBW32_RS00170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00170"
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="WP_077972231.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.4e-21, bitscore:
                     75.6, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.1e-20,
                     bitscore: 72.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 3.7e-18, bitscore:
                     64.2, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILVTGAAGFIGSHFVRHILSDSYPELAGAHVTALDKLTYAGNP
                     ENLAEIAYHPRYAFLHGDICDPHTVTEALRGIDLVVHFAAESHVDRSIADSAAFITTNV
                     LGTHILLQSALDTGVSRFVHVSTDEVYGSIAEGARAEDHPLDPNSPYAASKASSDLLAL
                     AFHRTHGLDVRVTRCSNNYGPYQHPEKVIPLFVTNLLEGLRLPVYGDGLHRRDWLHVDD
                     HCRAIAAVGSRGRAGEVYNIGGGTELSNVGLTHHILDIFDADWSVVEQIQDRAGHDRRY
                     SVDTRKIREELGWAPRISFASGLAETVAWYRENGSWWKTLKA"
     aSDomain        17271..17807
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GBW32_RS00170_NAD_binding_4.1"
                     /evalue="3.20E-20"
                     /label="GBW32_RS00170_NAD_binding_4.1"
                     /locus_tag="GBW32_RS00170"
                     /protein_end="183"
                     /protein_start="4"
                     /score="64.2"
                     /tool="antismash"
                     /translation="VTGAAGFIGSHFVRHILSDSYPELAGAHVTALDKLTYAGNPENLA
                     EIAYHPRYAFLHGDICDPHTVTEALRGIDLVVHFAAESHVDRSIADSAAFITTNVLGTH
                     ILLQSALDTGVSRFVHVSTDEVYGSIAEGARAEDHPLDPNSPYAASKASSDLLALAFHR
                     THGLDVRVTRCSNNYG"
     gene            complement(18718..19185)
                     /locus_tag="GBW32_RS00175"
                     /old_locus_tag="GBW32_00175"
     CDS             complement(18718..19185)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00175"
                     /product="hypothetical protein"
                     /protein_id="WP_077972229.1"
                     /transl_table=11
                     /translation="MLTPSRHTTPVVLASAVALLLAGCGSDGSDNSNSGAPQKSHGPTR
                     SATPPSKAPTSSPPPAKTSSPPKAVDGTNLAACGDGTCEVEVKTGDVISVKSSSGAKEF
                     MITGLSEDGPTVAVGPFDNPMTTTVNGGVEINGVSLEITPLNGKRAIAKVS"
     misc_feature    complement(19165..19180)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     misc_feature    19585..19599
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            complement(19673..20281)
                     /locus_tag="GBW32_RS00180"
                     /old_locus_tag="GBW32_00180"
     CDS             complement(19673..20281)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131463.1"
                     /locus_tag="GBW32_RS00180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00180"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_077972227.1"
                     /transl_table=11
                     /translation="MPRITKEDKARNRENILEAAGRMFRSQGIDAVGIAELMKEAGLTH
                     GGFYNHFASKSDLAVEVCGASFAASLGVLARTIEDGPGQGGSPLERVVSGYLSTAHRDA
                     PDGGCPSASLVTDAGRHSEAVQSTYAEGVEGYLTGFAAEFVRETEDKGQELDPDEARHR
                     AIRLLSEMVGALMLARAVCHVEPELSDEILRTGRSHALD"
     misc_feature    20349..20364
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.42"
                     /tool="antismash"
     misc_feature    20351..20365
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.52"
                     /tool="antismash"
ORIGIN
        1 gagtccgacg atgatcacgt ggtcgaactc cagccccttg gcggagtgca tggtcgacag
       61 tgccacgttc accgggccgg tgggccattc tccggtacgc gtgagcggga cccaggccag
      121 cccagcggca ttcagccgtg ccttgacctc gctgaaccag ccgccacctc tggcatggag
      181 gatggcgacg gactcgtcgt tttccgggcc gaggtcgttg aggaactgca cgacgtggtc
      241 catctgctgg gtgaaggtcc cctggaccaa caagggcttg tcgccgtcct cctcgcacga
      301 ggagagatcc gggatcgctg cgtcgtccgt ggtctctacg tcgcgcagca gggaggccgc
      361 gaaggcagcg acctggcggg tgttgcggaa gttcttcgat agcagcttgc tgttgtgcgg
      421 ccccacgctc aagccgactt cagtccaggt gaagcgctgg ggatagatgc gctgagccgc
      481 gtcaaggacg aaagtaaggg tgtgctcgtc ggtgacgtgg ttgaggaccg cccggacctg
      541 gttggcagag aagtcctggg cctcgtccac gacgaccacg tggtacgggt cgtcggcgcg
      601 cttggccgcc atggcgttgg ccaggtcgga ccagtcccag gcctgctgct ccttcttcca
      661 ctcctggaaa ggaaggatca cctcgtccag caaccgacgg cgcagcgaag ccggcatgcg
      721 cggggaacgg ccacggccct gacgctcgca ctcgatgtac tcgaccaggc ggtccggcat
      781 gaagcggccc aggacatagt ccacttcaga gcgcgcaaag tcctctgaca gaggcaaggc
      841 cgcagccaag gagtccagtc tcccgttgtg ctcgtgctct tgaaggatcc gctcgtaaga
      901 cacttggtcg gtggcccatt tggcgaaggt ggagatcctc agctcaacgt cattgccctt
      961 gatctgttcc ttggtcagtt cctcgatgta gccgcgcaga gtcttgttgt aggtgaggac
     1021 caggacccgg acggggccgt cgatgaaacc gtcacggcgg cgccgctgcc agaaaccgac
     1081 ggtgaacttc agccggtaga gggcagtggt ggttttgccg ctaccggcgg cgccccgtac
     1141 gacgaacact cctggcttgt gattgttgat caccgtgagc tgctcgctcg tcggctgcgc
     1201 cggcctgaga accttcatcc gcgcggtccc tccccaagac tcgctccccc gaacctgagg
     1261 ccatgttaga agcgactgac gccatgcggg ctgaagatag gacaagcagc ctgtcgcaac
     1321 agtgtcggat agcgacacgt cgaccactca ttcgggtgaa cgctgctgag gtcccctttc
     1381 cccggaggaa gcaggtactt ggctgttcgg cgtggagcag caagctgtca aagggcgggg
     1441 cgaggccgtc atcgagacgg cgtttgccac cgctgccggg caagtgacgc agctgtcatg
     1501 ggatcaagcg gcggcgacgg tgcggttcga ggacctggca ccggtatccg cgttcccggt
     1561 tgtgccgggc cgccggtggg ggccgggctg gtggtggtcg gcgaccaccg gacggcacgt
     1621 cgtgcacggt tcggccgcga tgcgcaccca gctgatggtc ctggaccggg atccggacgt
     1681 gaccggcctg tccgcgcgcc cggtacggct gctgtggcgg gacatgtccg acgggcgggt
     1741 gcgctcgtgg gtgccccagc tcttcgcccg gcacgcggac ggcaccggcc tgctcgccga
     1801 ctgcccctcc agcccgacca cgggtgggga cagggcgcag cgggcccgga tggtcctgga
     1861 ggcggcgtgc gcgcgcgtgg gctgggccta ccggcgcctc gagccgcctc cgacggtggt
     1921 ggcggcgaac ctgcggtggc tggccggcta ccgccacccc cgcaatcaag gcccgcccgg
     1981 gataggggca gcgctggccg aagtcttcgc cgcgccccgg ccgctggccg acggtgcggc
     2041 cgcggtgggg gatcccctgc aggtcctgcc ggctgtctat cacgcgctgt ggtgcgggca
     2101 tctgacgaca tctcttgatg agccactgca cgaggaagcg ctgatctgcg ctgacgcaga
     2161 acgcagtggt gagggtgttg aaaagcggct gggcgccggg ggcggccagg gaaggggtga
     2221 gtgcggtggg cggcacacgc ggtaagagcg ggcgaccggt ggtggaggtc ggtgcgcacg
     2281 tcacctaccg ggggcagacc tggcaggttg ccgccttgca ggggccgcgg gtctacctgg
     2341 tacaggaggc cggcactgaa gtgagcctgc tgctggggcg gttgttcgcc gacccggact
     2401 tcgaagtggt gggcgcacgg gcaccggata cggtgccgca gtgggggctg ttcgaggcgg
     2461 ttccggtggc ggcccagcag cgggcgctgg cctggctgcc ccatatccgg gaggtcgaga
     2521 ccgggtggcc gcaccctgag ggcggccgtg acggccacag gatgcggccg gagtacgacc
     2581 ccgagcggtg gacgctggcg cagcgggagg cggccaaggc gaaggaactg acggcgctcg
     2641 gcttcgcccg ggtgacgtct acgacggtgc agcggatgcg gcacgcctac cgcaagcaag
     2701 gcttgtgggg actggtcgac aaacgcgcgg tgccggcccg tggccgtcat ccgacgggat
     2761 atgccgacga gcgggtcgtg gccgcggtgc tggaagggct acggcgccag cggggccggt
     2821 cgaaggggac ggtgaaggga ctgcaggtac tggtcgggca gatccttcag gacacccatg
     2881 ggcgcggggt ggtggagatg ccgtcacgct cgacgttcta ccggctggtg agcgtgctgg
     2941 ccgacccggc cgaccgcccg gggctcccgg cgcgtaccgg cagcacgccc gcccgcgcct
     3001 catcggggcc gccggtggtg ctgcggcccg gggagcaggt gcagatcgac accacacgcc
     3061 tggacatcat ggccgtcctg gaggacggca gccttggccg gccggagctg acgatcgccg
     3121 tcgacgtcgc cacccgctcc atcctggccg ccgtcctgcg cccgcacagc accaaagccg
     3181 tcgacgccgc cctgctcctg gcggagatgg ctgttccgca tcctgcccgg ccggcctggc
     3241 ccgcagcgct gtatctgtcg cacgcccagg tgccgtatca gcggatgctg tcgctggacg
     3301 aacgcctgga aggcgcagcc gcgcggccgg tggtcgtgcc cgagacgatc gtcgtcgacc
     3361 gcgggaagat ctacctctcg cagggcttcg tcgccgcctg cgagaccctc ggggtgagcg
     3421 tccagcccgc ccctccgaga cggccgcagg ccaagggcgt ggtagagcgg accttcggct
     3481 cggtcaacga cctgttctgc cagcacgtcg ccggccacac cggctccaac ccccagcgcc
     3541 gcggctttgc gacagcggcc gaagcacggt ggacgatccc gcagctgcag gacttcctcg
     3601 acgaatggat cacctgcggc tggcagaacc ggccccacga cggactgcgc caccccgtcc
     3661 tgcccaaaac cgccctcaca ccgaaccaga tgtgggccgc gctgatcacc atcagcggct
     3721 acgtccccgt gccgctgacc ggggccgact acctcgaact gctgcccgtg cgctggcagc
     3781 ccatcaccga acgcggcatc cgcctcgact accgcaccta caaccacgac atcctcgacc
     3841 cccaccgcag ccagcactcc cccgtcacgg ccagagacgg caaatgggag gtccaccaca
     3901 acccccacga cgcccgccag atctgggtcc gcctcaccga cggacacctc cacgaaatcc
     3961 cctggatcca ccgcgaccac gtccaccagc ccttcaacaa caccatctgg cgccacgtcc
     4021 agaccgaggt cgaacaacgc ggagaccgcg agcagcatga ggccgacctc gccggcgccc
     4081 tcgaccagct cctgcgccgc acccgccacc ccgccgagac cgagcagaaa acccgccgcc
     4141 gcagagcccc ccgctccgat acggcagcgc agttgcccga tctgccaggc cagcggcgcc
     4201 ccctcgacgc agagaccgcc ccgggcccgg caccggactg gagcgagagc ctggacgacc
     4261 tcatcagcgt cgacaccccg gcccagaccg gcacgagcga gccggagggc gcgggcgggc
     4321 tgccgggagc ggcaggcggg tacgggctgt gggacgccga agcggaagcc gagcaatggt
     4381 gaacacctca tcgcgtacga acgatgcgcc ggccggcaag ccgccgacgg cgggccggtc
     4441 tcccggtgac gcggcgaacg ccgacggcga cacccctgat ccgcagcctc ggtcggtgac
     4501 cacctgggac ggcttccagg ccttcgccac cacccccgta gcagcccctc ccccgccggc
     4561 gcgccgcccc gcggtctcga ggaacggctg gcctaccact cgcggttcgt caccgtgcgc
     4621 acccccgcca tcgacgccct cgccaagaac gtgcgcaccc tgatgatcct cggccgccac
     4681 cagaccgtca ccgcccggcc ctcactcatc gtcaccggcc ccgcaggcgc cgggaaaacc
     4741 accgcgctgc tccaggtcgg gcgcgcctgc cacctcgccc acacccgccg ccacggtgcc
     4801 gcggcaacag ccggtcaggc cccggtcgcc tacgtcctgg tcccgcccgc cgccagcgcc
     4861 aaaaccctcg ccgccgaatt cgcccgctac ctcggcatcc ccgtcgccgc cggcatgtcc
     4921 caggcccaga tcacgaacgc cgtctgccac acctacacag ccgcccgggt ccagctggtc
     4981 ctgatcgacg aaatccaccg cctcaacccc cgcaccacca ccggcgccca gagcgccgac
     5041 ctgatcaaag acctcaccga acgcatcggc gccaccttcg tctacgccgg tatcgacgtc
     5101 accaccaccc ccctgttcac cggcgtacgc ggctcccagc tcgccggccg cgcctccctc
     5161 atcgactgcg ccgccttccc cgcccgcctc ggcgacgaag agcccttccg caacctcgtc
     5221 aacgcgatgg aaggcgcgct cgacctgcgt catcaccgga ccggaaccct gcctcaactg
     5281 gccccatacc tgcaccagcg caccgccggc cgcatcggca gcctcgcccg cctcatccgc
     5341 caggccgcca tcaccgccct catcgacggc accgaacgca tcaccaaaac ctcgctcgac
     5401 gcgatccgcc tcgaccacct cgccgaacag caccaacgcc cccgggcccg gagcaccaac
     5461 accccgtgag cccgacagac gccgcggccc gcgctgcggc aggggccggc accgcacagc
     5521 gcagcgtcgt caccacagag ccatggcatc ggatcccatt cgccgcgccg ggcgtgttcc
     5581 gcgtacggcc actgccccgc gaagcgaccg cctcatacgc ccaacgcctc gccgacacct
     5641 accagctgac gctcccccag ctcctcgacg gctccggcat caccctccac cgccacggca
     5701 cgccaccgac agccgaactc atcctcactc cgggcgctgc ccggcgcctc gccgtcctgg
     5761 cccgcaccgc gctcccccag ctgacgtgcg ccctgcccca tctccctctc agcgacaccg
     5821 cccacgacac cgaagcggcc gcgaactgga aacgactcga tgccgggcaa caacccgtcc
     5881 gcgcctgcac gctgtgcacc cgccaccgaa gtcacaacgc caccgacacc gcctggatcc
     5941 acccaccgcc gcaccaactg gtatgcccac gccaccacca agccgccccc gacccacgac
     6001 tcacctccac cgtccacacc cgagacgtgc ccgagctcgc cgccgcgcac cacgcccacc
     6061 agcgcctgct acggcatccc cgcgccgcca ccgcctggat cacagcgcgc gccatcacca
     6121 cccgctggta cgaccaccag caacacctca cccaccgctg gcacacccgc ctgacccagc
     6181 tccgggcgac taacccccac gtgaccacca caggcagcgc ctccccagcc ctgctaaccc
     6241 gcggcctgat cacctacccc gaaaccgtcg ccctcgcccg cgtactcacc accctgccga
     6301 gcgggcacca ccacaccacc aacggcgccc tcaccctcat cgcccgccga ctcgccctgg
     6361 cccatctggc ccccagcgcg aacgaccccc tccgcgtctt cctcacccac acacgccact
     6421 gaccagcatc atccagaacc ccaagcccac agaaaacaca gccgccttta gacgtgccac
     6481 cacacctacg aaacacccaa gatcaatccc acacacctac cgaaccgcag gccaagcacc
     6541 cgaacaccca aaccgaacac acccaccagc ccacacccac cccacccgaa cgcaaccact
     6601 ccaaccgtcc cgaatcccac cccaaaagac ggaacaccag accagaacag cccaaccacc
     6661 tgaagaccaa tggcccgtca cacgggcaca caggccattc ggggatggat gttcatggag
     6721 cgagtcgccg tgcgaagtgg gcatcccggg cgcgcgggca gccttgggat ccgcaggggt
     6781 cctcgccgcc ggtccaccgg cctggggagg tcagctgacg aggatgttga ccgaattgga
     6841 cgggaccccg cccgcgatcg tgcgcatgac cttctcgccc ttgtacatga acgagtcgta
     6901 aatcggcccg taggtaccgt cggccctggt cttcgcggtc ttgccgttga cgtcgaacca
     6961 ttttttcccg atgagttgct ggagctgtac ctcggtgccg gtgctcagcc ccggagtgct
     7021 gccggtgtag gccacgttct tgtgcaccgc gatcttgctg ggcttgacct ggaggttgtt
     7081 gctcgcggcg agggttgtct cgtgggcagc cgacgcggcg ggtgcgtggg cggttgccgt
     7141 agcgccgatg ccgccgccta cgagcagtgc ggccgaggcc gcgccgacga agaacccgcg
     7201 gctcacccgg tgaccgattg tatgaatcat ggtgcattcc tcccaaggaa tctgtccact
     7261 acgagatgtg tgtcccattc aggaggctag ggactcactg cgagacaccg gccgctcgga
     7321 aggaaaagcg agccggaaac cacccgaacg gctcaactcg accggcgtgt tgcccacgcc
     7381 aagcccgtag cccagggctg atctgaccga ctcgggaggc ccgcgaccgt gcgccctcac
     7441 tcgatcgccc cgccggaggg ccaccgaaag ggtgatcatc ggactgagcc gcgtgtgccg
     7501 tcgactgtgg ccgccagctc ctgggcccgg ccggcccgac ggcgaagcga cctacgacgg
     7561 caccgcacgg caccgcacgg cagcaggatg actcggtgac ctgggctgct gtctcttccg
     7621 ctgtccggcc gcccgctttc ccatggcatc gctggtgctg tgaccgcatg ggttcaccgg
     7681 gttggtggtc gcgacggttg gaggtgcggt ggcctcgtcc cgacgccggg gcagcgcacc
     7741 ggaccgtcgg ttgtttgtcg acttcctccc ggcggatggt tgagcgcccc ggagatgagg
     7801 gagtcggtgt tgaggaagaa cgggagcccc gcacccggtt ggtatggggc tcccgtgatg
     7861 cttgggggct caacgctcgc agcagaggcg gtcactgggc ggctggtggc cgacagcgtg
     7921 ggtcaggagc tcggagcggg ccgaagggac cgactccgcc ttcgaaccgg gcggtacagg
     7981 tgcgcgtacc tggatggacg gcgtcagcga gcgttgacga tcagggtgtg ggggctggtg
     8041 aggtagtccg tccagtgtgt gtcggtgggg gtgtcgtgga gggtgggcgc gtgccaggtg
     8101 acctcggtga acccggcatc gtggagggct tgccggtggg catggcggga ccaccggcgg
     8161 gctgtggcgg tgaaggtgtt cgcgtcgatc caggtgttca gctcgaccgg gtggccctcc
     8221 ggtggttccg ggctctcatg gtcgcccggg gtgagggtga agccgtaggg gcggtagtag
     8281 tcgtgggcgg tggcgaagtc ggggttgagg gtggctgtga caaatcggcc tcctggggcg
     8341 aggagcgttc gagcagtgcg gcagatgtcc gtgagctgcc gctgggtgga ggcgtagggc
     8401 aggacgtaga cgcagaccac ggcgtcgaag ctctggggag gcagcggggg tggctgggag
     8461 atgtcgtgca ggaggtaggt gatgccggat gcttcgcgac ggtcacgctg gtgggcatgc
     8521 gcgatcattc ccggggactg gtccagtcct gccacgacgc tggctccgcc gcgggccacg
     8581 aggcgcgcgt acagccccgc gccgcagccg aggtcgacca cacgcttgcc ccggaggtcg
     8641 ccgagggcgg cgaggaccga ggggatctcg atgtgccggc ggaagggcag ccctgcctcg
     8701 ctggcgccgt acagctcggc ggcttgatcg aaggcgtgtc cgggcatggt tggttctctc
     8761 ctgccatggg tggttctctt ctaccaggcg acgggcagac agacggggcg tacgaagatg
     8821 acgtcgttgc gccagggcac gtggtgctcg tcgacagcca ggtacaacgt ggggaagcgt
     8881 tcgaacaagg cgcgcagcgc tgcgtggagg tagccgcggg cgaactggga acccaggcag
     8941 tagtggggtc cggcaccgaa ggtcgctgcg gcgtcggcgc tccggtggat gttgaacttc
     9001 tccgggttct tgacgtgttc ggggtcccag gtggcggcct gcatggaggc gacgacggcc
     9061 tcgccccggc ggacgacctg gccgtgcagt tccacgtcct cggtggcgac gagggggaag
     9121 cccaggacgc cgttgtggta gtagcgcagt acttcctcgg ccgcgcgagg ccacagctcg
     9181 ggatcgtcga ggcattccct gagctgcttg cggtggagca tgaggatcat cgatccggtg
     9241 gtggtgggtg cgacgaccgc gtccgagccg gtgaagaaca gcacgaaggc cgtgcccgcc
     9301 atctcctcct cgctgatgac gcctgcgtcg acggcccgca ggagggcgcc gagcggatca
     9361 tcgagtaacg gtccgcgacg tcgcgccgcg atgacctctg ccacgaaccg ctggatctcc
     9421 tgggtctcgt tcccggtcgc ctccggtccc gcgctgatac tggcgccggt cgggaacagt
     9481 tgttcgtatg tgtcatagag ctcgggagga acgcccagga ccccgcgcat caccgtgagc
     9541 tggagccggg ccgcgtaggc ggtgacgagg tcggtcgggt tcggcccagc cgccatcgtg
     9601 tccagcaggg cgtgggcccg ttcacagatc agagggaggt acgtctgcgt cgcccgacgg
     9661 gtgaagtacg ggctgatgac ctgcttgacg cgggtgtggt cgggcggatc gaggttgaac
     9721 agtccgcgcc cgacggcggt cacgtcgtcg cccgagatac gggggctgcc gggggccagg
     9781 aggttgcggg agaaacgtgg gtcggcgaac acagcggcga tgtcgttctt acgggtcagc
     9841 atccagacac ggcttccgct gggcatcagc accggacaca ccggccggga cttctgtgcc
     9901 cactggatca atggcggcag cttgcccagg tcccccagcg gaaaggggta gggcagcgga
     9961 aggtcctgct cgatgcgcgg gttgctctgg tccgggacgc tcacgctgtc tcggcctccc
    10021 aggtggcgat cgcgtacctc tgcccggcgt gcggaacgag agacttgctc tcgccgctga
    10081 tcaggggacg gatcaatgcc atgtcgcggt ggtagaggaa tgccgacgag gggactccga
    10141 agatgtctgg cccttcggtc acgagcggaa gttcggccag ggcgtagctc acggccaggt
    10201 cgatcgcgga ctcgtccggc tcacgctgcc ccgccccgcg caccgcctcg cgcgcggtgt
    10261 cccggcaggc ccgtctgacc acagggtcgg aggcataggc gcgttggacc ctgtggcgtg
    10321 ctccggtgta cgcaggccgg gcgtgcaact gcgtccaggt gtgtacgtgg gtctgcggtt
    10381 cggtgactcc tgcgttccgc agcgcacgca gtgccgggtt gcgcagttga gtgttcgccc
    10441 gccgtgcgcg atgcaccgcc tcgccgacgg gaaagccggc ggcgaccagg gtgtgcgccg
    10501 cctcgtaccc cggggtgaag acgtcgattc tcggaaaacg cgagtgtgcc cacgtcacca
    10561 gcgcctcgat gtaacggcgg ctgtaccggc cgttccaggg gctcagcccg atcagggcat
    10621 gcccgcaccg gtcaggaaag aacgtttcac cccccggcag cggagagaga gtgaacatac
    10681 gcacgcagct ccttccctgg agcgattcgc cggttgaggg ggatcgctct tctcttcgcg
    10741 cgttcgcgtg gacgtttcgt gaggggcgcc cgtgcgggcg tctcccgaga ggcttcggac
    10801 cgtcacgccc gcccgccccg tcgccccggc ggacgcctcg cgcggccggg gcacccgcct
    10861 agggtgccgc gatgttgccc ttcggctctg gccgagcagc cgggcggcca tggcctgtcc
    10921 ccttccggcc cggtggccca gggggcatct cgcttcagcc cttactccgc ccggcccgcg
    10981 ccccgtcaac gaacagacgc cttcgccaca cttcccgacg gctgccgaca ggcgaggtca
    11041 ggcagccgag tcgatcggac cgagcaacca gttgcccgga gcgtcgaggt cggcgctgag
    11101 gaagaactcc gagagcgccg tccggtactc ctcatgactg atcaccccgt caccgtcccg
    11161 gtcgagtttc tggaaccccg ccgcggagtt gaccggcgag acactcagcc ggctcgccat
    11221 gctcacgtac tcttcccggc tcagaacccc gtctccgtcc gtgtccagcg cttgcatcgt
    11281 cgcgtcggcg atggcgagca ccgcgctgcc gaagttctcg ggggacgtga cgttggcctc
    11341 catcgcggca atgaactcat cgcgggagac ccggccgtcc ccgtcggtgt ctccgtgcgt
    11401 gaggagcaac tgccaccacc gctcgaacgc gtcgcgcatc gctgtggcgc tttcgcgctc
    11461 gccctgggcc agtgccgcga acagccccgc catcgcctgc atgtcgccct gggtgagctg
    11521 cccgtcccgg tcctggtcga accagtcgaa gagggtgttg aacttctgac tcttcactgc
    11581 actcgtcatt gcggttctcc ctcgattccc cagtccgcgc cggatacgcg gcggcgttcc
    11641 ggacactagg agcaacccga agccgcaatc caaccaaccc ggaaaccgtc actcgactgg
    11701 gctaagcaga aacgttgcgc ccagttgctc ccctgggggc tttgcgctgg ctgctcaggc
    11761 ctggcgcgct caagtggccg atgtgccacc gctgttcaac aggtcagcgt ggacctgggc
    11821 cgggggcggt agccgtgggc cttgcgcgaa cggtggttga gttcgtgagc gaaggtgtcc
    11881 aggtcggcct ggctgaacgg gcggaggtcg gcgcccttgg ggaggtactg ccgaagcagc
    11941 cggggttgtc gcgcgacgcc cctgccaggt gccgtcggag gccgccgtgt gctggagcac
    12001 ttgagcgagg ccaggtggca acggggcatc cctgacgtgg cgttgaccgg acccgtgagt
    12061 gttccggcca gtccccgctt cgtgagctgt gcccgttcga cctggagagg ccggtcacgc
    12121 gtcggccacc ttgtggaggg tgtcgctggt gtactggacc tcctccataa gacgtgcggc
    12181 ttcgatgacc aggacaccgt agggcctggt ggggtcggcc agagggagcg cctgggcctt
    12241 ggcttcgtcg acgagttcgt ggcgtcgctc ctgggcgccg tcggcgagac cggcgagccg
    12301 ctccgtctgc tggggcaggg tcgtctcgtc cagggtgctc agtgcgaggg cgatctccga
    12361 gacggcttcg aggaaggccg cgtagcggtc caggaagtgg aggtagcggt agtcgttctc
    12421 ctctttccgc cactggtcga gactgcgtgt cagagaggcc agttgggcga gtgtgcgttc
    12481 cagggcgtcg agcaccgact cgaacctctg gaacccgagc cggccgcggt tgcgccgcag
    12541 cagccgccgg gggttgtagt ggacgctctc cttggcggtg ttcaggccga cccgggcctg
    12601 ggcgatcaga ttccccgtcc gggcagcgtg ctcgcgccac cgcgtggtgg tgtccgcgtc
    12661 cagggtgccg gcgcgcagga cggggggcat gtcacaggtc agttcgtaga gtgcgcgggc
    12721 gagggcgcgg atgctgtatt cagcgctgcg gtagcgcatc ggcgggacga tgatgatgtt
    12781 gactgacgtg ccgacagcgc agccgatgag gacaagcagc acgatctgcc cgagctggct
    12841 gacgcgggtc atatcgtcgg tggcgctggt gtacgtggag aacgcgaaga acgcggcggt
    12901 ggccacctgc ggtccttgtg cgcccagtac ccgcgagcgc cctatggaca gagtgatcac
    12961 cgccaccagg gcaaaggtca gcaggtcgga acctgtgagg aagcccagca ccgcctgaac
    13021 ggccacgcca gcggtgaccg cgcccacgta acgcagggat tgcgtcacgg actggtagac
    13081 ggtgacgtac atgatcgtca ccgcggagaa cggcgcgaac gccggcgact gcgcgtcgag
    13141 ggcgtagaag gagatcatcc aggcgattgt ggccgccagg gtgcttttgc cgatcaggat
    13201 cagggcatgc cgctcggaac ccgcgtagcg caaggcccac gcccaccact gtgccgtccg
    13261 tgcgaaccgt ccctgcgtcc cttgcccggc cctcgcacca cccaccatgc gcgactcgct
    13321 ctctctccga ttcccggacc ggtgcccacg tgaccgccag cagtgcgatc aggctgcctg
    13381 cccgcccagg cggccttggc cggtgggaac accaccacat gcaggtagcc cccggactcg
    13441 tccatgaaac gtgtcgggga cccgtgcggg tacgtcaggg tctctccgcc acctcggatc
    13501 tgttcgcgcg acagacgtgc tgcggtgttc ctactgcctc cgaagaacgc ggtccggtgg
    13561 tcacgcatcc ccgccgagtg gccacagccg gccggggacg ggctgggcgg aagcggcggt
    13621 gcgccatcct tgcgtgtggc ctcccagcgc tcgatcagct ccttcttcca tccaaccgac
    13681 agctcttgtc tcacaaaggt gcgtcgtccc aggccggcaa ggtgctgtcg tgtttcctca
    13741 acaccggtcg gcgccccgga cggatcggtg tggctcaggg tggccacggc gcacgacatc
    13801 ggcgccgatg cccagagcag ccagcgtcgc gaccgcgccc tgtgcgtgcg cgaagcgttc
    13861 gccgcgaccc cagtacgggt cgttgagacc cacaaggaga cgcacctcgt cggtgatcag
    13921 ggcgaatccg acgccgagcg gcaggctcaa aagccgctcc cggcgtccgg gccgtacgag
    13981 cagcgcggtg cagcccacgg cgaatccgat ggctattccc ggtaggaagt ggtgcacgcg
    14041 tacggtgttc ttgaggggga tctccatcag gaggcgggtc aggccacgta tcctgggcag
    14101 tgctcgccgc cgttcacgca cgtagttgat ggtccgcgaa gtcgcgatgg tgatggcgaa
    14161 gctcgtggac aggccgagca gaacccgctc cggtcctggt gtgctgttgt acccgtcgat
    14221 gatccgctgt gctcccgcca ccggcccctg ctgtcggtgt ccctccctcc gcgactcctg
    14281 ccggggcttg tggtgctgct ccgcctgcgg accgggttcc ccattcgtcg gcaacatgct
    14341 gctcctcttc gcgttgtaga ggtggcgcct acccgccact gaagacgtcc catacgtgag
    14401 aggagggagc gtgcgcggat gcgcccgttc tctcccgcga cggcggccga ggtcggcgag
    14461 gctctcaact cccgtcacac gcgcatggag cagccgcgta gtccccgcac ctccaccccc
    14521 acctccatcg aacggttcat cgaggacgaa ttcgctcccc ggtaccgcgc ggcctgaccg
    14581 actgaaagcc ctggcggctc ggccggggcg gggagcgtgg ggaccgcagc cagctcgcgg
    14641 gtcgggcggc aacggcgagg ccacccgacc acagcgcagt gatagggtat aactattgca
    14701 agaataagtc gccgaaagcc ggttcaggaa tcttcgagga tgcccatgaa actgttgttc
    14761 agcgctaccc ctgcctttgg tcacatactg cccttggtcc cgctcatgca ggcggcggtc
    14821 agtgacggcc acgccgtcgg actgctgtcg agtgcggaat tcagggccgc ggtcggtgag
    14881 gagcttcctc cggaggtcga gtttctcgac gccggagtga tgcctgccgt gttctccgag
    14941 gaggcgggcc gtcgcaccgg tgccgacgtc ttccatccga caccttcgac catcggtgag
    15001 atcttcggag gtgcccgcgt cgacctcgct gtcgaggaga gcatctacca ggccaccgat
    15061 tgggccccgg acctgatggt cgccgagccg ttcgacgcgg tcggtccact cgtcgccgcc
    15121 cggctcggtg tcggatggca catggccggt atcggcccgg ctctgccctc ggtgatcacc
    15181 gatgaggtcg agcgcgtggc ctcgacccgg tacgagcggc tgcgacttcg cccggtcgcg
    15241 gcctcaagct acatcgatcc gtgcccgcgg caactgcagg atccggagtg gtccccggcc
    15301 gtccaggtga gggcggtccg gccccaggcc caccgtcgac cgaaggacgt cgcgctcgac
    15361 ctgtccggct tcggtgagcc cggcaagccg acggtgcttg tcaccttggg caccatcttc
    15421 tccgacccgg agactctcgc cgcggctgtg gcggctgtcg cggataccgg ggtcaatgtg
    15481 atcgccacgc tcggctcgtc cctgcggcat tccaccgcga gccaggcgac ccccgccgac
    15541 tcggcgaacg tacggtacgt gccttttgtt ccactggacc agttgctcga aagggttgat
    15601 ctggtagttg gcgccggagg cgtcgggaca gttgtcggca ccctcgcgca cggcctcccg
    15661 atggtgctgt ggccccaagg agccgaccag cccatcaacg ccgcacgcgc cgccgcatca
    15721 ggtgcgtcgg tcacggtcga ctcggcggag gggatttctc cggccgtggc ggacgtgctc
    15781 aggaatggca cgtaccgcga ccgggcccag gaaatcgcga ccggaatcgc caagttgccc
    15841 ctggcggcca cggtgatctc ggaaatcacc catccgtagc aggagcaggc gtcagcggtc
    15901 gcattcgtac cgtcaccagc gaaagcaacg ggaatcggtg gtgatcgccg ctccttgcgt
    15961 ctgatatccg accatcggcg gccgactgtc catcccggtt gagcacaccc ctgtcccggt
    16021 tgagcacacc gcgggtacat cgggtggagc gggtgcactg ccaccgaatc cgtttacgac
    16081 cgcgacagca ggaccccttg gtgctgctcg gttcggttcg agccttcgag cctccgggtc
    16141 accaaggggc gttccccttt gcgtcgcgag gaatggagac cacacgacct ggaatcttct
    16201 gggtattcgt cctcacgggt tcccctgggc tctccctgcc gcatccgaaa tctccctggc
    16261 ccccgcatca gcaccaccac atcagcaatg aacgcctcgg aggaaacgct gtgttcgaga
    16321 catcgctgga ccgccccaaa atgaagggga taatcctcgc cggaggcagt gccactcgac
    16381 tgtatccgct gaccggtgcg ctttccaagc agcagctgcc cgtttacgac aaacccatga
    16441 tctactaccc gctgtcagta ctgatgctgg ccggtatcca agacgtcctc gtcatctcct
    16501 cgcatcaaca cgtggaaacg ttccagttca tgctcggcga cggctcacgt atcggcatac
    16561 acatcgatta cgccgttcag gacgagcctc gcggcgtcgc cgacgcgttc cgggtcggcg
    16621 acaagcacgt cggcaatgac cgggtggcgc tcgttctggg tgacaatgtc tttcacggcc
    16681 ctggattcac caccgtgctg cgtcggggca tgcaacgcct cgacggatgt gaactgttcg
    16741 gctacccgtc cagaacgcta cggcgtcgcc gaaatcgacg cgcgggggca cttgttgtcg
    16801 ctccaggaga aaccgcgata tccccgttcc gacctcgcgg tcaccggact gtacttctac
    16861 gacaacgatg tgatcgaact ggcgaaggaa ctcaagcctt ccgcgcgcgg cgaactggag
    16921 ataaccgaca tcaacctcgc ctatttgacg caaggccggg cgcggctgac cgagctcggt
    16981 cgcggtttcg cctggctgga cctggggacc ccggactccc tgctccaggc cggccaatac
    17041 gtccagcttc tggagcagcg tcaaggcgta cgcatcgcct gtatcgagga gatcgccttg
    17101 cgtatgggtt ccatcgatgc tgacacctgt taccggctgg gcgaggagct ggccgcctcc
    17161 agctacggga actacctcat ggagaccgcc gcacgattcg gagcgacatc cgcaggctga
    17221 ccatttcccc cttcacagca cggcagaaag gcgattctat gcgcatcctg gtaaccgggg
    17281 cagcgggttt catcggctca cacttcgtca ggcacatcct gtcggactcc tacccggagc
    17341 tggcaggtgc ccacgtcacc gcgctcgaca agctgaccta cgccggaaac ccggaaaacc
    17401 tcgccgagat cgcataccat ccccgctacg ctttcctgca cggcgacatc tgcgacccgc
    17461 atacggtcac cgaggctctg cgcggcatcg atctggtggt gcacttcgcc gccgagtcgc
    17521 acgtcgaccg ctcgatcgcg gactccgccg cgttcatcac caccaacgtc ctgggcacgc
    17581 acatactgtt gcagtcggcg ctggacacgg gcgtgagccg attcgtccac gtctccaccg
    17641 acgaggtgta cggatcgatc gccgagggcg cccgcgccga ggaccacccg ctggacccga
    17701 attcccccta cgcggcgtcc aaggcctcct ccgacctcct ggcgctcgcc ttccaccgta
    17761 cgcacggcct cgatgtccgc gtgacgcgct gctccaacaa ctacggtccc taccagcatc
    17821 ccgagaaggt catcccgctg ttcgtcacga atctgctgga gggcctgcgg ctgcccgtgt
    17881 acggcgacgg cctccaccgg cgcgactggc tgcacgtgga cgaccactgc cgagccatcg
    17941 ccgcggtcgg cagccggggc cgggcgggag aggtctacaa catcggcggt ggaacggaac
    18001 tgtccaacgt cggactcacc caccacatcc tcgacatctt cgatgccgat tggtccgtgg
    18061 tcgaacagat acaggatcga gcggggcacg accgccggta ctccgtggac acccggaaga
    18121 tacgcgagga gctgggatgg gctccccgta tctccttcgc ttccgggctg gcggagactg
    18181 tcgcctggta ccgcgagaac ggatcatggt ggaagacgct gaaggcgtga cacccagtat
    18241 ccgttcgccc atgacgggcc tcaatacccc gtcatcatgt ctctcgtcgt gctcgcctcc
    18301 gcggctttcg tggtgttcgg gccctggcag ttcgaggaga gttggggggc ccgtcggtct
    18361 cagggagagc agagccgtca ccgtgtacta cgcccccgga aaaccggagc gggcgaacgc
    18421 gcgaccgccc ccggtagcca agagcgcccg cggcaacgag tgcggggagt tcaaggggat
    18481 gacgccggtg cggcggcgcg gacgcgaagg ccgatcggac ggcgagagcg agcggacgat
    18541 cggctgatag tggggcggca cgacatcgtc gttctcatca gcgtcacgcc gtagggcaca
    18601 tgcctgtacg tgggggcgac gcgaggggcc tgcggcacca ctcggggtac aggaagggga
    18661 ccgtccttgc gccgggacgc tgcccgattt gtgccccctg actgcctccg cacctcctca
    18721 ggagaccttg gcgatcgcac gcttgccgtt cagcggagtg atctccagcg agaccccgtt
    18781 gatctccaca ccaccgttca ccgtcgtggt catcgggttg tcgaacgggc cgacggccac
    18841 cgtcggcccg tcctcgctca aacccgtgat catgaactcc ttcgcgccgg agctgctctt
    18901 gacgctgatc acgtcgccgg ttttgacctc gacctcgcag gtaccgtcac cacaggcggc
    18961 gaggttggtg ccgtcgaccg ctttcggggg tgacgaggtt ttggcgggcg gtggtgacga
    19021 ggtgggcgcc ttggacggcg gagtggcgga tcgggtcggc ccgtgcgact tctgtggagc
    19081 gccgctgttc gagttgtcac tgccgtcgct tccgcatccc gcgagcagga gggcgacggc
    19141 ggaagccagt acgacaggtg tggtgtgacg tgagggtgtc agcatcgagc gtgtggtctc
    19201 ccggatctgg ttcggcgggc acgagggcgg cccacggcga caggaaacag tgtggcagtc
    19261 catccttgcg cacgatgcac caactacgct tccttgccgg gcagttgacg acccgttgtt
    19321 gcaaatcagg agctcaatcg ccgcgtgaat ccaatgccgc accaacgtcc gggatcgact
    19381 gtcactcgtg tgggggatcg gcggcggcac cgaagggcag gaagcccgcg gaaaggtatg
    19441 gagaccggat gtgtgggcgc gccggggtat cgcgcgtgct ccctcctccc gcaaggcggt
    19501 tcgagtgcca tcaaagatgg ccgtcggcgt cctgggcggc ggccgccact tcctccccct
    19561 gggcgtttga ggactctctc agagcgacgt gatcggccac ggtcaccatc cagtgactga
    19621 ccaggtaccg gtgagccagg acagccggaa cgaacgggtc ggtcacgccg cgtcaatcga
    19681 gggcgtgact acgaccggtc cgcaggatct cgtcggagag ctcgggctcg acgtggcata
    19741 cggcgcgggc gagcatcagc gccccgacca tctcgctgag caggcggatg gcccggtggc
    19801 gggcctcgtc cgggtcgagt tcctgcccct tgtcctccgt ctcacgcacg aactcggcgg
    19861 cgaacccggt gagataaccc tcgacacctt cggcgtacgt gctctgcacg gcttcgctgt
    19921 gccgtccggc gtcggtgacc agggaggccg aggggcaacc gccgtccggg gcgtcgcggt
    19981 gcgccgtgga caagtacccg gaaacgacgc gctccagcgg tgaaccgccc tggccggggc
    20041 cgtcctcgat cgtccgggcc aggaccccga gcgaggcggc gaacgaagcg ccgcacacct
    20101 ccacggccag gtcgctcttg gaggcgaagt ggttgtagaa gccgccgtgg gtgagtccgg
    20161 cttctttcat cagctcggcg atgccgaccg cgtcgatgcc ttgcgagcgg aacatgcggc
    20221 ccgccgcttc gaggatgttc tcccgattgc gggccttgtc ctccttggtg atccgcggca
    20281 ttgccgtcct ctctcgtcgc tctcgacttg acctttttga tgacgcctct catcatagca
    20341 ggagcttcaa tgacactcgt catcaatagc tcatggaaac ggagcgtcat gcgcgccatc
    20401 gcctacgacc agcacggaga agccgggcag gtactacgcc tgagcgaaca gccggacccc
    20461 gctgcgccca cggccgggca ggtgcgggtg cgggtagtgt cccggccgat ccaccccggt
    20521 gatctggcgg gagtggaggg cctcctgggt ggaccgcggc agcggttcgc cacgccacgg
    20581 atccccggcc tggaggggat gggcgtcgtc gagaccgtcg gcgcgggcgt gcgggatctg
    20641 cggcccggtc agcgggtggc gttcttcccg gtgccggc
//
