LOCUS       NZ_CP045177            12200 bp    DNA     linear   CON 25-NOV-2024
DEFINITION  Streptomyces tsukubensis strain AT3 plasmid pST1, complete sequence.
ACCESSION   NZ_CP045177
VERSION     NZ_CP045177.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tsukubensis
  ORGANISM  Streptomyces tsukubensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 18766)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Complete genome sequence of Streptomyces tsukubaensis AT3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18766)
  AUTHORS   Cao,G., Zhong,C., Fu,J., Zhang,P., Liu,M. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 18877 Jinshi Road, Jinan 250062,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2019
            Assembly Method :: SOAPdenovo v. OCT-2019; SPAdes v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 997.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009296025.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:40:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,005
            CDSs (total)                      :: 6,919
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 76 of 228
            Pseudo Genes (incomplete)         :: 184 of 228
            Pseudo Genes (internal stop)      :: 10 of 228
            Pseudo Genes (multiple problems)  :: 38 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:10:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6566
            Orig. end                         :: 18766
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045177.1.
            Bacteria and source DNA available from Dr. Guangxiang Cao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12200
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..12200
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12200
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10678](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10678)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     misc_feature    complement(760..775)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.34"
                     /tool="antismash"
     misc_feature    1159..1168
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            1411..2244
                     /locus_tag="GBW32_RS00030"
                     /old_locus_tag="GBW32_00030"
     CDS             1411..2244
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129558.1"
                     /locus_tag="GBW32_RS00030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00030"
                     /product="TnsA-like heteromeric transposase endonuclease
                     subunit"
                     /protein_id="WP_227024937.1"
                     /transl_table=11
                     /translation="MEQQAVKGRGEAVIETAFATAAGQVTQLSWDQAAATVRFEDLAPV
                     SAFPVVPGRRWGPGWWWSATTGRHVVHGSAAMRTQLMVLDRDPDVTGLSARPVRLLWRD
                     MSDGRVRSWVPQLFARHADGTGLLADCPSSPTTGGDRAQRARMVLEAACARVGWAYRRL
                     EPPPTVVAANLRWLAGYRHPRNQGPPGIGAALAEVFAAPRPLADGAAAVGDPLQVLPAV
                     YHALWCGHLTTSLDEPLHEEALICADAERSGEGVEKRLGAGGGQGRGECGGRHTR"
     gene            2225..4381
                     /locus_tag="GBW32_RS00035"
                     /old_locus_tag="GBW32_00035"
     CDS             2225..4381
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010069771.1"
                     /locus_tag="GBW32_RS00035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00035"
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="WP_179120366.1"
                     /transl_table=11
                     /translation="MGGTRGKSGRPVVEVGAHVTYRGQTWQVAALQGPRVYLVQEAGTE
                     VSLLLGRLFADPDFEVVGARAPDTVPQWGLFEAVPVAAQQRALAWLPHIREVETGWPHP
                     EGGRDGHRMRPEYDPERWTLAQREAAKAKELTALGFARVTSTTVQRMRHAYRKQGLWGL
                     VDKRAVPARGRHPTGYADERVVAAVLEGLRRQRGRSKGTVKGLQVLVGQILQDTHGRGV
                     VEMPSRSTFYRLVSVLADPADRPGLPARTGSTPARASSGPPVVLRPGEQVQIDTTRLDI
                     MAVLEDGSLGRPELTIAVDVATRSILAAVLRPHSTKAVDAALLLAEMAVPHPARPAWPA
                     ALYLSHAQVPYQRMLSLDERLEGAAARPVVVPETIVVDRGKIYLSQGFVAACETLGVSV
                     QPAPPRRPQAKGVVERTFGSVNDLFCQHVAGHTGSNPQRRGFATAAEARWTIPQLQDFL
                     DEWITCGWQNRPHDGLRHPVLPKTALTPNQMWAALITISGYVPVPLTGADYLELLPVRW
                     QPITERGIRLDYRTYNHDILDPHRSQHSPVTARDGKWEVHHNPHDARQIWVRLTDGHLH
                     EIPWIHRDHVHQPFNNTIWRHVQTEVEQRGDREQHEADLAGALDQLLRRTRHPAETEQK
                     TRRRRAPRSDTAAQLPDLPGQRRPLDAETAPGPAPDWSESLDDLISVDTPAQTGTSEPE
                     GAGGLPGAAGGYGLWDAEAEAEQW"
     gene            4495..5469
                     /locus_tag="GBW32_RS00040"
                     /old_locus_tag="GBW32_00040"
     CDS             4495..5469
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550093.1"
                     /locus_tag="GBW32_RS00040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00040"
                     /product="ATP-binding protein"
                     /protein_id="WP_077974392.1"
                     /transl_table=11
                     /translation="MTTWDGFQAFATTPVAAPPPPGAPPRGLEERLAYHSRFVTVRTPA
                     IDALAKNVRTLMILGRHQTVTARPSLIVTGPAGAGKTTALLQVGRACHLAHTRRHGAAA
                     TAGQAPVAYVLVPPAASAKTLAAEFARYLGIPVAAGMSQAQITNAVCHTYTAARVQLVL
                     IDEIHRLNPRTTTGAQSADLIKDLTERIGATFVYAGIDVTTTPLFTGVRGSQLAGRASL
                     IDCAAFPARLGDEEPFRNLVNAMEGALDLRHHRTGTLPQLAPYLHQRTAGRIGSLARLI
                     RQAAITALIDGTERITKTSLDAIRLDHLAEQHQRPRARSTNTP"
     gene            5466..6422
                     /locus_tag="GBW32_RS00045"
                     /old_locus_tag="GBW32_00045"
     CDS             5466..6422
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00045"
                     /product="TniQ family protein"
                     /protein_id="WP_227024938.1"
                     /transl_table=11
                     /translation="MSPTDAAARAAAGAGTAQRSVVTTEPWHRIPFAAPGVFRVRPLPR
                     EATASYAQRLADTYQLTLPQLLDGSGITLHRHGTPPTAELILTPGAARRLAVLARTALP
                     QLTCALPHLPLSDTAHDTEAAANWKRLDAGQQPVRACTLCTRHRSHNATDTAWIHPPPH
                     QLVCPRHHQAAPDPRLTSTVHTRDVPELAAAHHAHQRLLRHPRAATAWITARAITTRWY
                     DHQQHLTHRWHTRLTQLRATNPHVTTTGSASPALLTRGLITYPETVALARVLTTLPSGH
                     HHTTNGALTLIARRLALAHLAPSANDPLRVFLTHTRH"
     gene            complement(6811..7230)
                     /locus_tag="GBW32_RS00050"
                     /old_locus_tag="GBW32_00050"
     CDS             complement(6811..7230)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS00050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GBW32_00050"
                     /product="hypothetical protein"
                     /protein_id="WP_143621617.1"
                     /transl_table=11
                     /translation="MIHTIGHRVSRGFFVGAASAALLVGGGIGATATAHAPAASAAHET
                     TLAASNNLQVKPSKIAVHKNVAYTGSTPGLSTGTEVQLQQLIGKKWFDVNGKTAKTRAD
                     GTYGPIYDSFMYKGEKVMRTIAGGVPSNSVNILVS"
     misc_feature    complement(7216..7230)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.89"
                     /tool="antismash"
     gene            complement(8004..8747)
                     /locus_tag="GBW32_RS00055"
                     /old_locus_tag="GBW32_00055"
     CDS             complement(8004..8747)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.5"
                     /locus_tag="GBW32_RS00055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00055"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_077974357.1"
                     /transl_table=11
                     /translation="MPGHAFDQAAELYGASEAGLPFRRHIEIPSVLAALGDLRGKRVVD
                     LGCGAGLYARLVARGGASVVAGLDQSPGMIAHAHQRDRREASGITYLLHDISQPPPLPP
                     QSFDAVVCVYVLPYASTQRQLTDICRTARTLLAPGGRFVTATLNPDFATAHDYYRPYGF
                     TLTPGDHESPEPPEGHPVELNTWIDANTFTATARRWSRHAHRQALHDAGFTEVTWHAPT
                     LHDTPTDTHWTDYLTSPHTLIVNAR"
     misc_feature    8728..8743
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.5"
                     /tool="antismash"
     gene            complement(8781..10004)
                     /locus_tag="GBW32_RS00060"
                     /old_locus_tag="GBW32_00060"
     CDS             complement(8781..10004)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.5"
                     /locus_tag="GBW32_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00060"
                     /product="cytochrome P450"
                     /protein_id="WP_077974355.1"
                     /sec_met_domain="p450 (E-value: 8.7e-19, bitscore: 57.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVPDQSNPRIEQDLPLPYPFPLGDLGKLPPLIQWAQKSRPVCPV
                     LMPSGSRVWMLTRKNDIAAVFADPRFSRNLLAPGSPRISGDDVTAVGRGLFNLDPPDHT
                     RVKQVISPYFTRRATQTYLPLICERAHALLDTMAAGPNPTDLVTAYAARLQLTVMRGVL
                     GVPPELYDTYEQLFPTGASISAGPEATGNETQEIQRFVAEVIAARRRGPLLDDPLGALL
                     RAVDAGVISEEEMAGTAFVLFFTGSDAVVAPTTTGSMILMLHRKQLRECLDDPELWPRA
                     AEEVLRYYHNGVLGFPLVATEDVELHGQVVRRGEAVVASMQAATWDPEHVKNPEKFNIH
                     RSADAAATFGAGPHYCLGSQFARGYLHAALRALFERFPTLYLAVDEHHVPWRNDVIFVR
                     PVCLPVAW"
     misc_feature    complement(9366..9368)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10001..10678)
                     /locus_tag="GBW32_RS00065"
                     /old_locus_tag="GBW32_00065"
     CDS             complement(10001..10678)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF028025.5"
                     /locus_tag="GBW32_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00065"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_077974352.1"
                     /sec_met_domain="CDPS (E-value: 1.5e-52, bitscore: 168.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTLSPLPGGETFFPDRCGHALIGLSPWNGRYSRRYIEALVTWAH
                     SRFPRIDVFTPGYEAAHTLVAAGFPVGEAVHRARRANTQLRNPALRALRNAGVTEPQTH
                     VHTWTQLHARPAYTGARHRVQRAYASDPVVRRACRDTAREAVRGAGQREPDESAIDLAV
                     SYALAELPLVTEGPDIFGVPSSAFLYHRDMALIRPLISGESKSLVPHAGQRYAIATWEA
                     ETA"
     gene            complement(11038..11589)
                     /locus_tag="GBW32_RS00070"
                     /old_locus_tag="GBW32_00070"
     CDS             complement(11038..11589)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009999225.1"
                     /locus_tag="GBW32_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GBW32_00070"
                     /product="EF-hand domain-containing protein"
                     /protein_id="WP_077974349.1"
                     /transl_table=11
                     /translation="MTSAVKSQKFNTLFDWFDQDRDGQLTQGDMQAMAGLFAALAQGER
                     ESATAMRDAFERWWQLLLTHGDTDGDGRVSRDEFIAAMEANVTSPENFGSAVLAIADAT
                     MQALDTDGDGVLSREEYVSMASRLSVSPVNSAAGFQKLDRDGDGVISHEEYRTALSEFF
                     LSADLDAPGNWLLGPIDSAA"
     gene            complement(11796..12029)
                     /locus_tag="GBW32_RS37595"
     CDS             complement(11796..12029)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GBW32_RS37595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_405517597.1"
                     /transl_table=11
                     /translation="MPRCHLASLKCSSTRRPPTAPGRGVARQPRLLRQYLPKGADLRPF
                     SQADLDTFAHELNHRSRKAHGYRPRPRSTLTC"
ORIGIN
        1 agtccgacga tgatcacgtg gtcgaactcc agccccttgg cggagtgcat ggtcgacagt
       61 gccacgttca ccgggccggt gggccattct ccggtacgcg tgagcgggac ccaggccagc
      121 ccagcggcat tcagccgtgc cttgacctcg ctgaaccagc cgccacctct ggcatggagg
      181 atggcgacgg actcgtcgtt ttccgggccg aggtcgttga ggaactgcac gacgtggtcc
      241 atctgctggg tgaaggtccc ctggaccaac aagggcttgt cgccgtcctc ctcgcacgag
      301 gagagatccg ggatcgctgc gtcgtccgtg gtctctacgt cgcgcagcag ggaggccgcg
      361 aaggcagcga cctggcgggt gttgcggaag ttcttcgata gcagcttgct gttgtgcggc
      421 cccacgctca agccgacttc agtccaggtg aagcgctggg gatagatgcg ctgagccgcg
      481 tcaaggacga aagtaagggt gtgctcgtcg gtgacgtggt tgaggaccgc ccggacctgg
      541 ttggcagaga agtcctgggc ctcgtccacg acgaccacgt ggtacgggtc gtcggcgcgc
      601 ttggccgcca tggcgttggc caggtcggac cagtcccagg cctgctgctc cttcttccac
      661 tcctggaaag gaaggatcac ctcgtccagc aaccgacggc gcagcgaagc cggcatgcgc
      721 ggggaacggc cacggccctg acgctcgcac tcgatgtact cgaccaggcg gtccggcatg
      781 aagcggccca ggacatagtc cacttcagag cgcgcaaagt cctctgacag aggcaaggcc
      841 gcagccaagg agtccagtct cccgttgtgc tcgtgctctt gaaggatccg ctcgtaagac
      901 acttggtcgg tggcccattt ggcgaaggtg gagatcctca gctcaacgtc attgcccttg
      961 atctgttcct tggtcagttc ctcgatgtag ccgcgcagag tcttgttgta ggtgaggacc
     1021 aggacccgga cggggccgtc gatgaaaccg tcacggcggc gccgctgcca gaaaccgacg
     1081 gtgaacttca gccggtagag ggcagtggtg gttttgccgc taccggcggc gccccgtacg
     1141 acgaacactc ctggcttgtg attgttgatc accgtgagct gctcgctcgt cggctgcgcc
     1201 ggcctgagaa ccttcatccg cgcggtccct ccccaagact cgctcccccg aacctgaggc
     1261 catgttagaa gcgactgacg ccatgcgggc tgaagatagg acaagcagcc tgtcgcaaca
     1321 gtgtcggata gcgacacgtc gaccactcat tcgggtgaac gctgctgagg tcccctttcc
     1381 ccggaggaag caggtacttg gctgttcggc gtggagcagc aagctgtcaa agggcggggc
     1441 gaggccgtca tcgagacggc gtttgccacc gctgccgggc aagtgacgca gctgtcatgg
     1501 gatcaagcgg cggcgacggt gcggttcgag gacctggcac cggtatccgc gttcccggtt
     1561 gtgccgggcc gccggtgggg gccgggctgg tggtggtcgg cgaccaccgg acggcacgtc
     1621 gtgcacggtt cggccgcgat gcgcacccag ctgatggtcc tggaccggga tccggacgtg
     1681 accggcctgt ccgcgcgccc ggtacggctg ctgtggcggg acatgtccga cgggcgggtg
     1741 cgctcgtggg tgccccagct cttcgcccgg cacgcggacg gcaccggcct gctcgccgac
     1801 tgcccctcca gcccgaccac gggtggggac agggcgcagc gggcccggat ggtcctggag
     1861 gcggcgtgcg cgcgcgtggg ctgggcctac cggcgcctcg agccgcctcc gacggtggtg
     1921 gcggcgaacc tgcggtggct ggccggctac cgccaccccc gcaatcaagg cccgcccggg
     1981 ataggggcag cgctggccga agtcttcgcc gcgccccggc cgctggccga cggtgcggcc
     2041 gcggtggggg atcccctgca ggtcctgccg gctgtctatc acgcgctgtg gtgcgggcat
     2101 ctgacgacat ctcttgatga gccactgcac gaggaagcgc tgatctgcgc tgacgcagaa
     2161 cgcagtggtg agggtgttga aaagcggctg ggcgccgggg gcggccaggg aaggggtgag
     2221 tgcggtgggc ggcacacgcg gtaagagcgg gcgaccggtg gtggaggtcg gtgcgcacgt
     2281 cacctaccgg gggcagacct ggcaggttgc cgccttgcag gggccgcggg tctacctggt
     2341 acaggaggcc ggcactgaag tgagcctgct gctggggcgg ttgttcgccg acccggactt
     2401 cgaagtggtg ggcgcacggg caccggatac ggtgccgcag tgggggctgt tcgaggcggt
     2461 tccggtggcg gcccagcagc gggcgctggc ctggctgccc catatccggg aggtcgagac
     2521 cgggtggccg caccctgagg gcggccgtga cggccacagg atgcggccgg agtacgaccc
     2581 cgagcggtgg acgctggcgc agcgggaggc ggccaaggcg aaggaactga cggcgctcgg
     2641 cttcgcccgg gtgacgtcta cgacggtgca gcggatgcgg cacgcctacc gcaagcaagg
     2701 cttgtgggga ctggtcgaca aacgcgcggt gccggcccgt ggccgtcatc cgacgggata
     2761 tgccgacgag cgggtcgtgg ccgcggtgct ggaagggcta cggcgccagc ggggccggtc
     2821 gaaggggacg gtgaagggac tgcaggtact ggtcgggcag atccttcagg acacccatgg
     2881 gcgcggggtg gtggagatgc cgtcacgctc gacgttctac cggctggtga gcgtgctggc
     2941 cgacccggcc gaccgcccgg ggctcccggc gcgtaccggc agcacgcccg cccgcgcctc
     3001 atcggggccg ccggtggtgc tgcggcccgg ggagcaggtg cagatcgaca ccacacgcct
     3061 ggacatcatg gccgtcctgg aggacggcag ccttggccgg ccggagctga cgatcgccgt
     3121 cgacgtcgcc acccgctcca tcctggccgc cgtcctgcgc ccgcacagca ccaaagccgt
     3181 cgacgccgcc ctgctcctgg cggagatggc tgttccgcat cctgcccggc cggcctggcc
     3241 cgcagcgctg tatctgtcgc acgcccaggt gccgtatcag cggatgctgt cgctggacga
     3301 acgcctggaa ggcgcagccg cgcggccggt ggtcgtgccc gagacgatcg tcgtcgaccg
     3361 cgggaagatc tacctctcgc agggcttcgt cgccgcctgc gagaccctcg gggtgagcgt
     3421 ccagcccgcc cctccgagac ggccgcaggc caagggcgtg gtagagcgga ccttcggctc
     3481 ggtcaacgac ctgttctgcc agcacgtcgc cggccacacc ggctccaacc cccagcgccg
     3541 cggctttgcg acagcggccg aagcacggtg gacgatcccg cagctgcagg acttcctcga
     3601 cgaatggatc acctgcggct ggcagaaccg gccccacgac ggactgcgcc accccgtcct
     3661 gcccaaaacc gccctcacac cgaaccagat gtgggccgcg ctgatcacca tcagcggcta
     3721 cgtccccgtg ccgctgaccg gggccgacta cctcgaactg ctgcccgtgc gctggcagcc
     3781 catcaccgaa cgcggcatcc gcctcgacta ccgcacctac aaccacgaca tcctcgaccc
     3841 ccaccgcagc cagcactccc ccgtcacggc cagagacggc aaatgggagg tccaccacaa
     3901 cccccacgac gcccgccaga tctgggtccg cctcaccgac ggacacctcc acgaaatccc
     3961 ctggatccac cgcgaccacg tccaccagcc cttcaacaac accatctggc gccacgtcca
     4021 gaccgaggtc gaacaacgcg gagaccgcga gcagcatgag gccgacctcg ccggcgccct
     4081 cgaccagctc ctgcgccgca cccgccaccc cgccgagacc gagcagaaaa cccgccgccg
     4141 cagagccccc cgctccgata cggcagcgca gttgcccgat ctgccaggcc agcggcgccc
     4201 cctcgacgca gagaccgccc cgggcccggc accggactgg agcgagagcc tggacgacct
     4261 catcagcgtc gacaccccgg cccagaccgg cacgagcgag ccggagggcg cgggcgggct
     4321 gccgggagcg gcaggcgggt acgggctgtg ggacgccgaa gcggaagccg agcaatggtg
     4381 aacacctcat cgcgtacgaa cgatgcgccg gccggcaagc cgccgacggc gggccggtct
     4441 cccggtgacg cggcgaacgc cgacggcgac acccctgatc cgcagcctcg gtcggtgacc
     4501 acctgggacg gcttccaggc cttcgccacc acccccgtag cagcccctcc cccgcccggc
     4561 gcgccgcccc gcggtctcga ggaacggctg gcctaccact cgcggttcgt caccgtgcgc
     4621 acccccgcca tcgacgccct cgccaagaac gtgcgcaccc tgatgatcct cggccgccac
     4681 cagaccgtca ccgcccggcc ctcactcatc gtcaccggcc ccgcaggcgc cgggaaaacc
     4741 accgcgctgc tccaggtcgg gcgcgcctgc cacctcgccc acacccgccg ccacggtgcc
     4801 gcggcaacag ccggtcaggc cccggtcgcc tacgtcctgg tcccgcccgc cgccagcgcc
     4861 aaaaccctcg ccgccgaatt cgcccgctac ctcggcatcc ccgtcgccgc cggcatgtcc
     4921 caggcccaga tcacgaacgc cgtctgccac acctacacag ccgcccgggt ccagctggtc
     4981 ctgatcgacg aaatccaccg cctcaacccc cgcaccacca ccggcgccca gagcgccgac
     5041 ctgatcaaag acctcaccga acgcatcggc gccaccttcg tctacgccgg tatcgacgtc
     5101 accaccaccc ccctgttcac cggcgtacgc ggctcccagc tcgccggccg cgcctccctc
     5161 atcgactgcg ccgccttccc cgcccgcctc ggcgacgaag agcccttccg caacctcgtc
     5221 aacgcgatgg aaggcgcgct cgacctgcgt catcaccgga ccggaaccct gcctcaactg
     5281 gccccatacc tgcaccagcg caccgccggc cgcatcggca gcctcgcccg cctcatccgc
     5341 caggccgcca tcaccgccct catcgacggc accgaacgca tcaccaaaac ctcgctcgac
     5401 gcgatccgcc tcgaccacct cgccgaacag caccaacgcc cccgggcccg gagcaccaac
     5461 accccgtgag cccgacagac gccgcggccc gcgctgcggc aggggccggc accgcacagc
     5521 gcagcgtcgt caccacagag ccatggcatc ggatcccatt cgccgcgccg ggcgtgttcc
     5581 gcgtacggcc actgccccgc gaagcgaccg cctcatacgc ccaacgcctc gccgacacct
     5641 accagctgac gctcccccag ctcctcgacg gctccggcat caccctccac cgccacggca
     5701 cgccaccgac agccgaactc atcctcactc cgggcgctgc ccggcgcctc gccgtcctgg
     5761 cccgcaccgc gctcccccag ctgacgtgcg ccctgcccca tctccctctc agcgacaccg
     5821 cccacgacac cgaagcggcc gcgaactgga aacgactcga tgccgggcaa caacccgtcc
     5881 gcgcctgcac gctgtgcacc cgccaccgaa gtcacaacgc caccgacacc gcctggatcc
     5941 acccaccgcc gcaccaactg gtatgcccac gccaccacca agccgccccc gacccacgac
     6001 tcacctccac cgtccacacc cgagacgtgc ccgagctcgc cgccgcgcac cacgcccacc
     6061 agcgcctgct acggcatccc cgcgccgcca ccgcctggat cacagcgcgc gccatcacca
     6121 cccgctggta cgaccaccag caacacctca cccaccgctg gcacacccgc ctgacccagc
     6181 tccgggcgac taacccccac gtgaccacca caggcagcgc ctccccagcc ctgctaaccc
     6241 gcggcctgat cacctacccc gaaaccgtcg ccctcgcccg cgtactcacc accctgccga
     6301 gcgggcacca ccacaccacc aacggcgccc tcaccctcat cgcccgccga ctcgccctgg
     6361 cccatctggc ccccagcgcg aacgaccccc tccgcgtctt cctcacccac acacgccact
     6421 gaccagcatc atccagaacc ccaagcccac agaaaacaca gccgccttta gacgtgccac
     6481 cacacctacg aaacacccaa gatcaatccc acacacctac cgaaccgcag gccaagcacc
     6541 cgaacaccca aaccgaacac acccaccagc ccacacccac cccacccgaa cgcaaccact
     6601 ccaaccgtcc cgaatcccac cccaaaagac ggaacaccag accagaacag cccaaccacc
     6661 tgaagaccaa tggcccgtca cacgggcaca caggccattc ggggatggat gttcatggag
     6721 cgagtcgccg tgcgaagtgg gcatcccggg cgcgcgggca gccttgggat ccgcaggggt
     6781 cctcgccgcc ggtccaccgg cctggggagg tcagctgacg aggatgttga ccgaattgga
     6841 cgggaccccg cccgcgatcg tgcgcatgac cttctcgccc ttgtacatga acgagtcgta
     6901 aatcggcccg taggtaccgt cggccctggt cttcgcggtc ttgccgttga cgtcgaacca
     6961 ttttttcccg atgagttgct ggagctgtac ctcggtgccg gtgctcagcc ccggagtgct
     7021 gccggtgtag gccacgttct tgtgcaccgc gatcttgctg ggcttgacct ggaggttgtt
     7081 gctcgcggcg agggttgtct cgtgggcagc cgacgcggcg ggtgcgtggg cggttgccgt
     7141 agcgccgatg ccgccgccta cgagcagtgc ggccgaggcc gcgccgacga agaacccgcg
     7201 gctcacccgg tgaccgattg tatgaatcat ggtgcattcc tcccaaggaa tctgtccact
     7261 acgagatgtg tgtcccattc aggaggctag ggactcactg cgagacaccg gccgctcgga
     7321 aggaaaagcg agccggaaac cacccgaacg gctcaactcg accggcgtgt tgcccacgcc
     7381 aagcccgtag cccagggctg atctgaccga ctcgggaggc ccgcgaccgt gcgccctcac
     7441 tcgatcgccc cgccggaggg ccaccgaaag ggtgatcatc ggactgagcc gcgtgtgccg
     7501 tcgactgtgg ccgccagctc ctgggcccgg ccggcccgac ggcgaagcga cctacgacgg
     7561 caccgcacgg caccgcacgg cagcaggatg actcggtgac ctgggctgct gtctcttccg
     7621 ctgtccggcc gcccgctttc ccatggcatc gctggtgctg tgaccgcatg ggttcaccgg
     7681 gttggtggtc gcgacggttg gaggtgcggt ggcctcgtcc cgacgccggg gcagcgcacc
     7741 ggaccgtcgg ttgtttgtcg acttcctccc ggcggatggt tgagcgcccc ggagatgagg
     7801 gagtcggtgt tgaggaagaa cgggagcccc gcacccggtt ggtatggggc tcccgtgatg
     7861 cttgggggct caacgctcgc agcagaggcg gtcactgggc ggctggtggc cgacagcgtg
     7921 ggtcaggagc tcggagcggg ccgaagggac cgactccgcc ttcgaaccgg gcggtacagg
     7981 tgcgcgtacc tggatggacg gcgtcagcga gcgttgacga tcagggtgtg ggggctggtg
     8041 aggtagtccg tccagtgtgt gtcggtgggg gtgtcgtgga gggtgggcgc gtgccaggtg
     8101 acctcggtga acccggcatc gtggagggct tgccggtggg catggcggga ccaccggcgg
     8161 gctgtggcgg tgaaggtgtt cgcgtcgatc caggtgttca gctcgaccgg gtggccctcc
     8221 ggtggttccg ggctctcatg gtcgcccggg gtgagggtga agccgtaggg gcggtagtag
     8281 tcgtgggcgg tggcgaagtc ggggttgagg gtggctgtga caaatcggcc tcctggggcg
     8341 aggagcgttc gagcagtgcg gcagatgtcc gtgagctgcc gctgggtgga ggcgtagggc
     8401 aggacgtaga cgcagaccac ggcgtcgaag ctctggggag gcagcggggg tggctgggag
     8461 atgtcgtgca ggaggtaggt gatgccggat gcttcgcgac ggtcacgctg gtgggcatgc
     8521 gcgatcattc ccggggactg gtccagtcct gccacgacgc tggctccgcc gcgggccacg
     8581 aggcgcgcgt acagccccgc gccgcagccg aggtcgacca cacgcttgcc ccggaggtcg
     8641 ccgagggcgg cgaggaccga ggggatctcg atgtgccggc ggaagggcag ccctgcctcg
     8701 ctggcgccgt acagctcggc ggcttgatcg aaggcgtgtc cgggcatggt tggttctctc
     8761 ctgccatggg tggttctctt ctaccaggcg acgggcagac agacggggcg tacgaagatg
     8821 acgtcgttgc gccagggcac gtggtgctcg tcgacagcca ggtacaacgt ggggaagcgt
     8881 tcgaacaagg cgcgcagcgc tgcgtggagg tagccgcggg cgaactggga acccaggcag
     8941 tagtggggtc cggcaccgaa ggtcgctgcg gcgtcggcgc tccggtggat gttgaacttc
     9001 tccgggttct tgacgtgttc ggggtcccag gtggcggcct gcatggaggc gacgacggcc
     9061 tcgccccggc ggacgacctg gccgtgcagt tccacgtcct cggtggcgac gagggggaag
     9121 cccaggacgc cgttgtggta gtagcgcagt acttcctcgg ccgcgcgagg ccacagctcg
     9181 ggatcgtcga ggcattccct gagctgcttg cggtggagca tgaggatcat cgatccggtg
     9241 gtggtgggtg cgacgaccgc gtccgagccg gtgaagaaca gcacgaaggc cgtgcccgcc
     9301 atctcctcct cgctgatgac gcctgcgtcg acggcccgca ggagggcgcc gagcggatca
     9361 tcgagtaacg gtccgcgacg tcgcgccgcg atgacctctg ccacgaaccg ctggatctcc
     9421 tgggtctcgt tcccggtcgc ctccggtccc gcgctgatac tggcgccggt cgggaacagt
     9481 tgttcgtatg tgtcatagag ctcgggagga acgcccagga ccccgcgcat caccgtgagc
     9541 tggagccggg ccgcgtaggc ggtgacgagg tcggtcgggt tcggcccagc cgccatcgtg
     9601 tccagcaggg cgtgggcccg ttcacagatc agagggaggt acgtctgcgt cgcccgacgg
     9661 gtgaagtacg ggctgatgac ctgcttgacg cgggtgtggt cgggcggatc gaggttgaac
     9721 agtccgcgcc cgacggcggt cacgtcgtcg cccgagatac gggggctgcc gggggccagg
     9781 aggttgcggg agaaacgtgg gtcggcgaac acagcggcga tgtcgttctt acgggtcagc
     9841 atccagacac ggcttccgct gggcatcagc accggacaca ccggccggga cttctgtgcc
     9901 cactggatca atggcggcag cttgcccagg tcccccagcg gaaaggggta gggcagcgga
     9961 aggtcctgct cgatgcgcgg gttgctctgg tccgggacgc tcacgctgtc tcggcctccc
    10021 aggtggcgat cgcgtacctc tgcccggcgt gcggaacgag agacttgctc tcgccgctga
    10081 tcaggggacg gatcaatgcc atgtcgcggt ggtagaggaa tgccgacgag gggactccga
    10141 agatgtctgg cccttcggtc acgagcggaa gttcggccag ggcgtagctc acggccaggt
    10201 cgatcgcgga ctcgtccggc tcacgctgcc ccgccccgcg caccgcctcg cgcgcggtgt
    10261 cccggcaggc ccgtctgacc acagggtcgg aggcataggc gcgttggacc ctgtggcgtg
    10321 ctccggtgta cgcaggccgg gcgtgcaact gcgtccaggt gtgtacgtgg gtctgcggtt
    10381 cggtgactcc tgcgttccgc agcgcacgca gtgccgggtt gcgcagttga gtgttcgccc
    10441 gccgtgcgcg atgcaccgcc tcgccgacgg gaaagccggc ggcgaccagg gtgtgcgccg
    10501 cctcgtaccc cggggtgaag acgtcgattc tcggaaaacg cgagtgtgcc cacgtcacca
    10561 gcgcctcgat gtaacggcgg ctgtaccggc cgttccaggg gctcagcccg atcagggcat
    10621 gcccgcaccg gtcaggaaag aacgtttcac cccccggcag cggagagaga gtgaacatac
    10681 gcacgcagct ccttccctgg agcgattcgc cggttgaggg ggatcgctct tctcttcgcg
    10741 cgttcgcgtg gacgtttcgt gaggggcgcc cgtgcgggcg tctcccgaga ggcttcggac
    10801 cgtcacgccc gcccgccccg tcgccccggc ggacgcctcg cgcggccggg gcacccgcct
    10861 agggtgccgc gatgttgccc ttcggctctg gccgagcagc cgggcggcca tggcctgtcc
    10921 ccttccggcc cggtggccca gggggcatct cgcttcagcc cttactccgc ccggcccgcg
    10981 ccccgtcaac gaacagacgc cttcgccaca cttcccgacg gctgccgaca ggcgaggtca
    11041 ggcagccgag tcgatcggac cgagcaacca gttgcccgga gcgtcgaggt cggcgctgag
    11101 gaagaactcc gagagcgccg tccggtactc ctcatgactg atcaccccgt caccgtcccg
    11161 gtcgagtttc tggaaccccg ccgcggagtt gaccggcgag acactcagcc ggctcgccat
    11221 gctcacgtac tcttcccggc tcagaacccc gtctccgtcc gtgtccagcg cttgcatcgt
    11281 cgcgtcggcg atggcgagca ccgcgctgcc gaagttctcg ggggacgtga cgttggcctc
    11341 catcgcggca atgaactcat cgcgggagac ccggccgtcc ccgtcggtgt ctccgtgcgt
    11401 gaggagcaac tgccaccacc gctcgaacgc gtcgcgcatc gctgtggcgc tttcgcgctc
    11461 gccctgggcc agtgccgcga acagccccgc catcgcctgc atgtcgccct gggtgagctg
    11521 cccgtcccgg tcctggtcga accagtcgaa gagggtgttg aacttctgac tcttcactgc
    11581 actcgtcatt gcggttctcc ctcgattccc cagtccgcgc cggatacgcg gcggcgttcc
    11641 ggacactagg agcaacccga agccgcaatc caaccaaccc ggaaaccgtc actcgactgg
    11701 gctaagcaga aacgttgcgc ccagttgctc ccctgggggc tttgcgctgg ctgctcaggc
    11761 ctggcgcgct caagtggccg atgtgccacc gctgttcaac aggtcagcgt ggacctgggc
    11821 cgggggcggt agccgtgggc cttgcgcgaa cggtggttga gttcgtgagc gaaggtgtcc
    11881 aggtcggcct ggctgaacgg gcggaggtcg gcgcccttgg ggaggtactg ccgaagcagc
    11941 cggggttgtc gcgcgacgcc cctgccaggt gccgtcggag gccgccgtgt gctggagcac
    12001 ttgagcgagg ccaggtggca acggggcatc cctgacgtgg cgttgaccgg acccgtgagt
    12061 gttccggcca gtccccgctt cgtgagctgt gcccgttcga cctggagagg ccggtcacgc
    12121 gtcggccacc ttgtggaggg tgtcgctggt gtactggacc tcctccataa gacgtgcggc
    12181 ttcgatgacc aggacaccgt
//
