LOCUS       NZ_CP045096            10399 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Streptomyces phaeolivaceus strain GY16 chromosome, complete genome.
ACCESSION   NZ_CP045096
VERSION     NZ_CP045096.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces phaeolivaceus
  ORGANISM  Streptomyces phaeolivaceus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10116136)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain GY16 isolated from leaves of Broussonetia
            papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10116136)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-AUG-2019
            Assembly Method                   :: HGAP v. v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009184865.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 02:51:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,113
            CDSs (total)                      :: 9,024
            Genes (coding)                    :: 8,642
            CDSs (with protein)               :: 8,642
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 112 of 382
            Pseudo Genes (incomplete)         :: 305 of 382
            Pseudo Genes (internal stop)      :: 23 of 382
            Pseudo Genes (multiple problems)  :: 53 of 382
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1971730
            Orig. end                         :: 1982129
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045096.1.
            Bacteria and source DNA available from Yunlin Zhao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="11"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(55..69)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            complement(430..1290)
                     /locus_tag="F9278_RS09320"
                     /old_locus_tag="F9278_09330"
     CDS             complement(430..1290)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338057.1"
                     /locus_tag="F9278_RS09320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09330"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_152167879.1"
                     /transl_table=11
                     /translation="MNSVPGRRSRILAATTAAAGLLLVAGCSSDGDGGSGTKTAAGGVE
                     LVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVALIDLVAEDLGVKQEILDTPFENFKTGA
                     FLNSGECDLAAAGMTITDERKKNVDFSDPYFEATQAVLAAKKADIRSFADLKGDGDYKV
                     GTQAQTTGEDYAKSQGIDSVSFESSDAVLNGLRTGQVDAVVIDYPVVQGWLKNAELADA
                     FEVAEQVNTGEEYGITVKKGNTALLAAIDKALADAKSDGTYKKLYEQWIGPYDESAASA
                     TPSAS"
     gene            1464..2564
                     /locus_tag="F9278_RS09325"
                     /old_locus_tag="F9278_09335"
     CDS             1464..2564
                     /NRPS_PKS="Domain: Aminotran_5 (74-301). E-value: 1.4e-27.
                     Score: 88.5. Matches aSDomain:
                     nrpspksdomains_F9278_RS09325_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477727.1"
                     /locus_tag="F9278_RS09325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09335"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_152167880.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-20, bitscore:
                     72.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFASVEDRVGRLLSTAQDVVITQGEALLPLE
                     GAIRGAAGPGTTALNVITGPYGQTFGNWLRDCGATVIDLAVPFHTAVTAEQIREAFAEH
                     PAIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFHLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKALGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACVERVEAEGLAAVMSRHASAAAATRAGTLALGGGLEPYVHEAADAAPVA
                     TTLRAPAGVDASELVAKALAADPALPLAAGGGALASEMIRVNHYGPDATPGVVHAALAG
                     LGAALAEFGAPVDPAGARRAATAAWA"
     aSDomain        1686..2366
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F9278_RS09325_Aminotran_5.1"
                     /evalue="1.40E-27"
                     /label="F9278_RS09325_Aminotran_5.1"
                     /locus_tag="F9278_RS09325"
                     /protein_end="301"
                     /protein_start="74"
                     /score="88.5"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAVTAEQIREAFAEHPAIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFHLDAVASVGAEPVLPDAWGVDLCVIGAQKALGGPAGVSA
                     ISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACVERV
                     EAEGLAAVMSRHASAAAATRAGTLALGGGLEPYVHEAADAAPVATTLRAPAGVDASELV
                     AKALA"
     misc_feature    2784..2799
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2918..2930
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            2954..3484
                     /gene="ectA"
                     /locus_tag="F9278_RS09330"
                     /old_locus_tag="F9278_09340"
     CDS             2954..3484
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004616.1"
                     /locus_tag="F9278_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09340"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_193241413.1"
                     /transl_table=11
                     /translation="MTAAQAEPLTEIVEGIRIDRPSVADGAALWRIAGDSGVLDLNSSY
                     SYLLWCRDFADTSAVARDESGEPVGFVTGYVRPERPDTLLVWQVAVDEAHRGLGLAAAL
                     LDGLTARVARQHSLTAVETTISPDNTASERLFASYAERHGARVERTVLFEAADFPDGPH
                     QPEVLHRIGPLSP"
     gene            3555..4826
                     /gene="ectB"
                     /locus_tag="F9278_RS09335"
                     /old_locus_tag="F9278_09345"
     CDS             3555..4826
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8.6e-73.
                     Score: 237.2. Matches aSDomain:
                     nrpspksdomains_F9278_RS09335_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="F9278_RS09335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09345"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_152167881.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-76, bitscore:
                     257.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVESVQGEGGINVARP
                     EWLRALAGLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRERGEQAEQAL
                     ISITEENLADVKEYRGRGLVWGIEFHEKARAGRIAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLRVLARAVRETV"
     aSDomain        3639..4652
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F9278_RS09335_Aminotran_3.1"
                     /evalue="8.60E-73"
                     /label="F9278_RS09335_Aminotran_3.1"
                     /locus_tag="F9278_RS09335"
                     /protein_end="366"
                     /protein_start="28"
                     /score="237.2"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVESVQGEGGINVARPEWLRALAGLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRERGEQAEQALISITEENLADVKEYRGRGLVWGIEFH"
     gene            5001..5399
                     /locus_tag="F9278_RS09340"
                     /old_locus_tag="F9278_09350"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="F9278_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09350"
                     /product="ectoine synthase"
                     /protein_id="WP_152167882.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-53, bitscore:
                     178.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLARERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTDQETGRSYTITPGTMYLLDGHERHTLRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5405..6292
                     /gene="thpD"
                     /locus_tag="F9278_RS09345"
                     /old_locus_tag="F9278_09355"
     CDS             5405..6292
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="F9278_RS09345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09355"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_152167883.1"
                     /sec_met_domain="PhyH (E-value: 2.6e-40, bitscore: 137.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTDLYPSRGATEVSVPRKDPVVWGSPGTPGPVSLAELQAYER
                     DGFLPVEQLIAPDEVAVYHRELERLVADPAIRADERSIVEPQSQEIRSVFEVHRISELF
                     ARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENHDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLRMQDAGTPSDEALTAM
                     ASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFVVFNSVENTAVEPFAAPIR
                     RPEFIGARDFTPVG"
     gene            complement(6391..7486)
                     /locus_tag="F9278_RS09350"
                     /old_locus_tag="F9278_09360"
                     /pseudo=""
     CDS             complement(6391..7486)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="F9278_RS09350"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09360"
                     /product="alkene reductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTAYDPVDLSGTRLANRIVMSPMPLPQFSASLEPGGPPSGRRGR
                     APHRAHRRVLRPARLGRPHRDRGRPAVPRGPGLPAHSRSARRGPGRRLAPGHRRRARGR
                     RHDLRPTDAHRPDRPPRAARGPAPGRCLGGRRAGAGVHARGPQGLRHPARADPRRGPRH
                     RPGLRDGRARRDRGRLRRGRTARGQRLSDPPVPRTRLQSAHRRLGRLRGEPHPVRRADR
                     AGRRGGRRRPPHRAAAVARQPLQRHQRARPRVHVHRARPGPGAARSGVSAHRRGPRAAR
                     ADPAAAQAVLRHLRAQPGDGRPHRTGRPGPRRGRHRRPGRLRSALPRQPGPPGPAEGGR
                     PVQPARPGDLLRRRRQGIHGLPGAV"
     misc_feature    7564..7579
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.57"
                     /tool="antismash"
     misc_feature    7569..7578
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.42"
                     /tool="antismash"
     gene            7627..8103
                     /locus_tag="F9278_RS09355"
                     /old_locus_tag="F9278_09365"
     CDS             7627..8103
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512203.1"
                     /locus_tag="F9278_RS09355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09365"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_152167884.1"
                     /transl_table=11
                     /translation="MHPTEKRSNALPSAARGGPVSHAVSRVARLHRNAAGRLLRGLGLH
                     PGQELVMMHLWESGPARQSDLIRLLDLDPSTVTKMLQRLEQAGHVRRRSDPADRRAVLV
                     EATEESGVLLERVEAAWAELEELTLAGLDAAERARFLRLLARVESNLCAESTGR"
     gene            complement(8188..9210)
                     /locus_tag="F9278_RS09360"
                     /old_locus_tag="F9278_09370"
     CDS             complement(8188..9210)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="F9278_RS09360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09370"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_152167885.1"
                     /transl_table=11
                     /translation="MTNDHDGVRELLAAWAVGALPAADQRTVPPHLTACESCAAEADRL
                     RDTVRLLDGEATPDAAPGAVNGSAGALLALALRARRPRAAEIAHHAAPYAAAVAGLQAL
                     VPELEGRWGTPVVHDWDAHATVAHLVAADEHLALRLGVGAVLPASHIPEGTSAGDAWAR
                     RTFDVIAHEHGRAPEETVATWAAQAAALLATPEAADPELAARAVTVMGLRLPVADHFVV
                     RAFEAWIHTDDIGRALGLAVPPPPDEHLWSLVRLAVRILGLALHDAPPVRFGVTGPTDT
                     GWILGTDTDPVHAELTLDPVDFCLLVGGRYAPDEVPRTITGDETAARTVLETAASLAWL
                     "
     gene            complement(9287..9895)
                     /locus_tag="F9278_RS09365"
                     /old_locus_tag="F9278_09375"
     CDS             complement(9287..9895)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="F9278_RS09365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_09375"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_152167886.1"
                     /transl_table=11
                     /translation="MVVPVEPTLEQPSIDVELHRRLVYGDESALRELYAAYGGLVRRVA
                     VRVTRSPAAAEDVAQEVFAQLWSRPYGFDARRGSLRTWLSMVAHRRAVDWVRGEARHRK
                     DARADDSALHAIPDTGPGPAEAVVDRERSLELHTALAELPRPQREVVHLAYFAGRTYRQ
                     AAVELGIPEGTAKTRLRSALRKLAESLADPPDPAAVRGA"
ORIGIN
        1 aggtgccgcc ggggatctcc gtgccgggga aggccagcgg cacggcgaca ccgatgaaga
       61 tgaagatcag cagggcgggc aggccccgga agatctcgat gtacacgccg gcgaaccagc
      121 ggtacgggcc gaccgacgac agccgcatca gcgcgatgac catgccgagg acgagtccga
      181 acacgaagcc ggacagcgtg tacagcacgg tgttcttgag cgcgagcgtg atgacgtcgg
      241 ggaacatctg ctcggcgatg tcccactggg cgaactggtt cctcagccgc gcccagtcgg
      301 cggacaccgc gaaggcgacg acggccgcga cgaagacgac gtactgcgcg ccccgggaca
      361 gggcgcgctt ccggctccgg ctgaggccct tccgcttcgg ctggagcgtg gtgtccgggt
      421 cggtcacgct catgaggcgg acggggtggc ggaggcggcg ctctcgtcgt acgggccgat
      481 ccactgctcg tacagcttct tgtacgtgcc gtcggacttc gcgtccgcca gcgccttgtc
      541 gatggcggcg agcagcgcgg tgttgccctt cttcaccgtg atgccgtact cctcaccggt
      601 gttgacctgc tcggcgacct cgaaggcgtc ggccagctcc gcgttcttca gccagccctg
      661 gacgaccggg tagtcgatga cgacggcgtc gacctggccg gtgcgcaggc cgttgaggac
      721 ggcgtcggag gactcgaagg agacggagtc gatgccctgg ctcttggcgt agtcctcgcc
      781 ggtggtctgc gcctgggtgc cgaccttgta gtccccgtcg cccttgaggt ccgcgaagga
      841 gcggatgtcg gccttcttgg cggcgaggac ggcctgcgtg gcctcgaagt acgggtcgga
      901 gaagtcgacg ttcttcttgc gctcgtcggt gatcgtcata ccggcggcgg cgaggtcgca
      961 ctcgccggag ttcaggaagg cgcccgtctt gaagttctcg aacggggtgt cgaggatctc
     1021 ctgcttcacg cccaggtcct cggcgacgag gtcgatcagc gccacgtcga agccctgcac
     1081 cttgccgtcg atctccgact ggaacggcgg gtacgggagg tgggtgcagg tcgtgagctg
     1141 accggccttg accagctcca ccccgccggc cgcggtcttg gtgccgctgc cgccgtcgcc
     1201 gtccgaggaa cagccggcca cgagcagcag accggccgcg gcggtggtgg cggccaggat
     1261 gcgggaccgg cgtccgggga ccgagttcac ggggagacct cctgtgggat gactgagaca
     1321 accgattata aggagatgtt tgactttccc aaaccaatcc gatggccatc ggggcgcctg
     1381 acctcggatg tcaccggtgc ggggtgtgcg ggtgccgtcg gtaccctcgg aaccatctac
     1441 cccgtcagtg aagagagcac cccgtgacac acccgtttct ggatctggcc ccgttgagcg
     1501 cggcgcactt cgcgtccgtc gaggaccggg tggggcggct gctctccacc gcgcaggacg
     1561 tcgtgatcac ccagggcgag gcgctgctgc cgctggaggg cgcgatccgc ggggccgccg
     1621 ggcccggcac gaccgcgctg aacgtgatca ccggcccgta cgggcagacc ttcgggaact
     1681 ggctgcggga ctgcggggcg accgtgatcg atctggccgt ccccttccac acggcggtca
     1741 cggccgagca gatccgggaa gcgttcgccg agcacccggc gatcgacttc gtgtcgctgg
     1801 tccacgcgga ggcggcgacc ggcaacacca acccggtggc ggagatcggc gaggtggtcc
     1861 gcgcgcacgg cgcgctgttc cacctggacg cggtcgcctc cgtcggcgcc gagccggtgc
     1921 tgccggacgc gtggggcgtc gacctgtgcg tgatcggggc gcagaaggca ctgggcggac
     1981 cggccggggt gtcggcgatc tcggtgagcg agcgggcctg ggcgcggatg gcggccaacc
     2041 cgagggcgcc gcgccgctcg tacctctccc tgctggactg gaaggagcgc tggatcgacg
     2101 gcggccggcg cgcgctcctg cacgcgcccg cccagctgga gatgctcgcc ctggaggcct
     2161 gtgtggagcg cgtggaggcg gagggcctgg cggcggtgat gtcccggcac gcgtccgccg
     2221 cggcggcgac ccgggcgggg acgctggcgc tgggcggcgg tctggagccg tacgtccacg
     2281 aggcggccga cgccgcgccg gtggccacga cgctccgggc gcccgccggg gtggacgcgt
     2341 cggagctggt cgccaaggct ctcgccgcgg accccgccct gccgctggcc gcgggcggcg
     2401 gcgccctcgc ctcggagatg atccgggtca accactacgg gcccgacgcg accccgggcg
     2461 tcgtgcacgc cgccctcgcg gggctgggcg cggccctcgc ggagttcggc gcgccggtcg
     2521 atccggcggg ggcccgccgg gcggcgacgg cggcctgggc gtgaccggag caccacttca
     2581 ccgagaaggc cgaaggaatc tagggaatct aagggaatcg agggaatata gggaattgca
     2641 gtcggccttt tgcggcagaa atgcagcgga atcaacagaa caggatcccg tattcttctg
     2701 cccgctcttc cctctcctaa acgcaaaggt ttcgcgaaca ctcacggggc taattcgagc
     2761 agatctcgtg agggttgtat acatgtgacc gactccacaa cgcgcccctt ttgtgcggta
     2821 atttacgggc aaacaccctc acatcgcccc cgtttccctg ggccgctcac gcccgcgtga
     2881 taacacgtgg acggcccata cgtaaccccg gggggcgatg caattcgaaa ttcccatggg
     2941 taaattcaag cccatgactg ccgcacaagc agaaccgctg acggaaatcg tggaagggat
     3001 tcggatcgac cgcccgagcg tggcggacgg cgccgcgctg tggcgcatcg ccggggactc
     3061 cggagtcctg gacctgaact cctcgtacag ctatctgctg tggtgccggg acttcgcgga
     3121 cacctcggcg gtggcgcggg acgagtccgg ggagccggtc gggttcgtga ccgggtatgt
     3181 gcggcccgag cggccggaca ccctgctcgt gtggcaggtg gccgtcgacg aggcccatcg
     3241 ggggctcggg ctcgccgccg cgctcctgga cgggctcacc gcacgggtcg cgcgacagca
     3301 ctccctcacc gcggtcgaga ccaccatctc gccggacaac accgcctccg agcggctgtt
     3361 cgcctcgtac gccgaacgcc atggcgcgcg cgtcgagcgg acggtcctgt tcgaggcggc
     3421 ggacttcccg gacggcccgc accagcccga ggtgctgcac cgcatcggcc cgctgtcccc
     3481 ctgagccccc cgcgcacccc cctcatcccc ctccctcacc tcccgcaccc ccacgcaccg
     3541 aggagcgatt cgacgtgacc atcacccagc ccgacctgag cgtcttcgag accctggagt
     3601 ccgaggtgcg cagttactgc cgcggctggc ccaccgtctt cgaccgcgcg cagggcagcc
     3661 ggatgttcga cgaggacggc cacacgtacc tggacttctt cgccggcgcc gggtcactca
     3721 actacggcca caacaacccg gtgctcaaac gcgccctgat cgactatctg gaacgggacg
     3781 gcgtcaccca cgggctcgac atgtcgacga ccgcgaagcg ggcgttcctg gagtccttcc
     3841 agaatctggt gctgcgcccg cgcgatctgc cgtacaaggt gatgttcccg gggccgaccg
     3901 gcaccaacgc ggtggagtcg gcgctgaagc tggcccggaa ggtgaagggg cgcgaggcca
     3961 tcgtgtcgtt caccaacgcc ttccacggca tgtcgctcgg gtcgctcgcc gtcaccggca
     4021 acgccttcaa gcgggccggg gcgggcgtcc cgctcgtgca cggcacgccg atgccgttcg
     4081 acaactactt cgacgggcag gtcccggact tcctgtggtt cgagcggctg cttgaggacc
     4141 agggctccgg gctgaacaag cccgccgccg tgatcgtcga gagcgtgcag ggcgagggcg
     4201 gcatcaacgt cgcccgcccg gagtggctgc gggcgctcgc cggactgtgc gagcggcagg
     4261 acatgctgct gatcgtcgac gacatccaga tgggctgcgg tcgcacgggc gccttcttct
     4321 ccttcgagga ggcgggcatc acgcccgaca tcgtgaccgt ctccaagtcc atcagcggct
     4381 acgggctgcc tatgtcgctg tgcctgttca agccggagct ggacatctgg gagcccggcg
     4441 agcacaacgg cacgttccgc ggcaacaacc ccgcgttcgt gacggcgacg gccgcgctgg
     4501 agacgtactg ggcggacggc tccgcgatgg agaagcagac ccgcgagcgc ggtgagcagg
     4561 ccgagcaggc gctgatctcg atcaccgagg agaacctcgc cgatgtgaag gagtaccggg
     4621 ggcgcggcct ggtgtggggc atcgagttcc acgagaaggc gcgcgccggg cggatcgcca
     4681 agcgggcctt cgaactcggg ctgctgatcg agacgtccgg cccggagagc gaggtcgtca
     4741 aactgctgcc cgcgctcacc atcacgcccg aggaactgga cgagggcctg cgtgtcctcg
     4801 cccgcgccgt acgggaaacc gtctgacctc gtacgggaaa ccgtctgacc tcgtacggga
     4861 aaccgtccga ccccgtacgg gaaaccgtcc gaccccgtac gggaaaccgt ccgaccccgt
     4921 acgggaaacc gtccgacccc gtacgggaaa ccgtccgacg ccgtacggga gttcatctaa
     4981 ctaggaggca tcgcagcacc gtgatcgtcc gttcgttcaa ggacatcgaa ggaaccgacc
     5041 ggcatgtgaa gtcggcgtcc ggcacctggg agagcaagcg gatcgtgctc gcccgggagc
     5101 gggtcgggtt ctccctgcac gagaccgtcc tgtacgcggg tacggagacg tccatgtggt
     5161 acgcgaacca catcgaggcc gtcgtgtgcg tcgagggcga ggccgagctc accgaccagg
     5221 agaccgggcg gagttacacg atcacgcccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcggatcaag gaggacttcc gctgcctctg tgtcttcaac ccgcccgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctatcccct gctgacggag gagggctgac
     5401 cctcatgacc acgctcaccg atctctaccc cagccgcggc gccaccgagg tgtcggtccc
     5461 gcgcaaggac ccggtcgtct ggggctcccc cggcaccccc ggcccggtct ccctcgccga
     5521 actccaggcg tacgagcgcg acggcttcct ccccgtcgag cagctgatcg cgccggacga
     5581 ggtcgccgtc taccaccggg agctggagcg gctggtcgcc gatccggcga tccgggccga
     5641 cgagcggtcg atcgtcgagc cgcagtcgca ggagatccgg tcggtgttcg aggtgcaccg
     5701 gatcagcgag ctgttcgcgc ggctggtgcg cgacgagcgg gtcgtcgggc gggcccggca
     5761 gatcctcggc tcggacgtct acgtccacca gtcgcggatc aatgtgaagc ccggcttcgg
     5821 cgcgtccggg ttctactggc actccgactt cgagacctgg cacgccgagg acggtctgcc
     5881 gaacatgcgg acggtgtccg tctcgatcgc gctgaccgag aaccacgaca ccaacggcgg
     5941 tctgatgatc atgccggggt cgcatcggac gttcctcggc tgcgccgggg ccaccccgaa
     6001 ggacaactac aagaagtcgc tgcggatgca ggacgccggc accccgtccg acgaggcgct
     6061 gacggccatg gcctccgagt acggcatcaa gctgttcacg ggcaaggccg gttcggcgac
     6121 ctggttcgac tgcaactgca tgcacggctc cggcgacaac atcacgccgt tcccgcgcag
     6181 caatgtcttc gtcgtcttca acagcgtgga gaacacggcc gtggagccct tcgcggctcc
     6241 gatccgccgg ccggagttca tcggcgcgcg ggacttcacc ccggtggggt gatcccgccg
     6301 ccccggtgac gggccgggct gtcgccgccc ccctcggcga cagcccggcc ctttcacgtg
     6361 cccggaccgg accggcgact acagtggcga ctacagcgcc gggtagtccg tgtatccctt
     6421 ggcgtcgccg ccgtagaagg tcgccgggtc gggctggttg tacgggccgc ccgccttcag
     6481 ccgggcctgg aggtccgggt tggcgaggaa gagctgaccg aaggcgacca ggtcggcggt
     6541 gccgtcctcg acgagggcca ggtcgtccgg tccggtgggg ccgtccgtcg ccgggttgag
     6601 cacgaaggtg ccggagaaca gcttgcgcag ccgctgggtc agctcgcgct gcccgggggc
     6661 ctcgccgatg tgcagatacg ccagaccgag cggctccagg gcctggacga gcgcggtgta
     6721 cgtggactcg gggtcgggct cgctgatgtc gttgaagggg ttgccgggcg acagccgcag
     6781 cgcggtgcgg tgggcgccga cggcctccgc gacggcccgc acggtccgca cggcgaaccg
     6841 gatgcggttc tcctcggagc cgccccagtc gtcggtgcgc agattggagc cgggtgcgag
     6901 gaactggtgg atcagatagc cgttggcccc gtgcggttcg accccgtcga agccggcctc
     6961 gatcgcgccg cgcgcggccg tcgcgaagtc ctggacggtg ccgaggacct cgtcgtgggt
     7021 cagctcgcgc ggggtgacga agtccttggg gccctcgtgc gtgtacacct gcccctgcgc
     7081 ggcgaccgcc gagacaccga ccgggtgcag gccctcgggc agcacggggt ggccgatccg
     7141 gccggtgtgc atcagttgga cgaagatcgt gccgccggcc gcgtgcgcgg cgtcggtgac
     7201 cgggcgccag gcggcgacct ggtcctcgtc gtgcagaccg ggagtgtgcg ggtagccctg
     7261 gcccacgtgg gacggctgga cgccctcggt cacgatgagg ccggccgagg cgcgctgggc
     7321 gtagtactcg gcggtgagcg cggtggggcg cccgccctcg gcggcccgat gggggtcccc
     7381 ccggttcgag cgaagccgag aactggggga ggggcatggg ggacatgacg atgcggttgg
     7441 cgagccgtgt gccggacagg tcgacggggt cgtaggcggt ggtcacggta actcccggtg
     7501 ggtgggttat gtggtcggcc gatcaatgca accggggagc actgtagccc attccttggt
     7561 cggccaacct ttttttgacc gagaggtacc ttgggcggtg agaccgagct gaccagggag
     7621 ttttccatgc accccaccga gaagcgttcg aacgcgctgc cgtccgcggc gcgcggcggg
     7681 ccggtcagcc acgcggtctc ccgggtggcc cggctgcatc gcaacgccgc cggacggctg
     7741 ctgcgggggc tggggctcca ccccgggcag gagctggtga tgatgcacct gtgggagagc
     7801 gggccggcgc ggcagtccga cctgatccgg ctgctcgacc tcgatccgtc gacggtgacg
     7861 aagatgctgc agcggctgga gcaggcgggg catgtgcggc ggcggtccga tcccgcggat
     7921 cggcgggccg tgctggtgga ggcgaccgag gagagcgggg ttcttctgga gcgggtggag
     7981 gcggcttggg cggagttgga ggagctgacg ttggccgggc tggacgccgc cgagcgcgct
     8041 cggttcttgc ggctgctggc ccgggtcgag tcgaaccttt gcgcggaatc gacggggcgt
     8101 tgaggggtcg tgggcatgtg cgggtccgtg ggggccggtc aggcggttcc ccgcgccccg
     8161 tacggggcgc tcctcacggg gcgcccctca cagccatgcc agtgacgccg ccgtctccag
     8221 tacggtcctt gccgcggtct cgtcgcccgt gatggtcctg gggacctcgt ccggggcgta
     8281 gcgaccgccg accaggaggc agaagtcgac ggggtccagg gtgagttcgg cgtggacggg
     8341 gtcggtgtcg gtgccgagga tccagccggt gtcggtgggg ccggtgaccc cgaagcggac
     8401 ggggggcgcg tcgtggaggg ccaggccgag gatgcggacg gccaggcgga ccagggacca
     8461 gaggtgttcg tcggggggcg gcgggacggc gaggccgagc gcgcggccga tgtcgtcggt
     8521 gtggatccac gcctcgaagg cccggaccac gaaatggtcg gcgaccggca gccgcagtcc
     8581 catcaccgtg acggcgcggg cggcgagttc ggggtcggcg gcctccgggg tggccagcag
     8641 cgcggcggcc tgcgcggccc aggtggcgac cgtctcctcc ggggcgcgcc cgtgctcgtg
     8701 agcgatgacg tcgaaggtgc gcctggccca ggcgtccccc gcggatgtcc cctccgggat
     8761 gtgcgaggcg ggcaggacgg ccccgacacc gaggcgcagg gcgaggtgtt cgtcggcggc
     8821 gaccaggtgc gcgacggtgg cgtgcgcgtc ccagtcgtgc acgaccggtg tgccccagcg
     8881 gccctccaac tccggtacca gcgcctggag tccggccact gcggcggcgt agggggcggc
     8941 gtgatgggcg atctcggcgg cgcgggggcg ccgggcgcgc agggcgagcg cgaggagggc
     9001 gccggcgctc ccgttcaccg cgccgggggc cgcgtcaggg gtcgcctcgc cgtccagcag
     9061 tcgtaccgtg tcgcggagcc ggtccgcctc cgccgcgcac gactcgcagg cggtcagatg
     9121 cggggggacc gtccgctggt cggcggccgg gagcgcgcca acggcccagg cggccagcag
     9181 ctcccgtacg ccgtcgtggt cgttggtcat cgcgcgccgc cctttcgccc tgccacgtcc
     9241 acgatacgca cgccccgcac ggtccggagg gtcgtgtgcg cccccatcac gcgcccctca
     9301 cggctgccgg gtccggtggg tccgcgaggg actccgccag tttgcgcagg gccgagcgga
     9361 ggcgggtctt cgcggtgccc tcgggtatgc ccagttcgac ggcggcctgg cggtaggtgc
     9421 ggcccgcgaa gtaggcgagg tggaccactt cccgctgggg gcgcgggagt tcggcgaggg
     9481 cggtgtgcag ttccagggag cgctcgcggt cgacgaccgc ctcggccggg ccggggccgg
     9541 tgtccgggat cgcgtgcagc gcggagtcgt cggcgcgggc gtccttgcgg tgccgggcct
     9601 cgccgcgcac ccagtcgacg gcccgccggt gggcgaccat ggacagccag gtgcgcagcg
     9661 agccccggcg cgcgtcgaag ccgtacggtc tgctccacag ctgggcgaag acctcctgcg
     9721 ccacgtcctc ggccgccgcc gggctgcggg tgacccggac ggccacccgc cggacgagtc
     9781 ccccgtacgc cgcgtacagc tcacgcagcg cggactcgtc cccgtacacc agccgccgat
     9841 gcaactccac atcgatggac ggctgttcga gcgtcggctc caccggcacc accacctcgt
     9901 gaagccttcc tagcgtcctg gcgcggtccg cgccagtggg tctcggaagt cagccggaca
     9961 gaacgccgag gagccggtcg acatggacac cgcctactga ggcaccgcgc acccgtccgg
    10021 cccgcaggcc tccgcgccct ccgccgccac gaccttcagc ggcgcgggcc gctcccccca
    10081 cgcctgggcg agcgccttct cgaagacctc ggcgggctgt gccccggaca ccccgtactt
    10141 ccggtccagc acgaagaacg gcacgccgtt cgcccccagc tccgccgcct cccgctcgtc
    10201 cgcgcggacg gcgtcggcgt acgcgtcccg gtcggccagc accttgcgcg cggcctcctc
    10261 gtcgagcccc gcctcgacgg ccagctcgac cagatacccg tccgcgtcgg cgtacacgga
    10321 ccgctcctcg gcgaaattcg cccggtagaa ggcctggagc agctcgctct gccggccctg
    10381 ctccttggcg aagtggatc
//
