LOCUS       NZ_CP045096            10411 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Streptomyces phaeolivaceus strain GY16 chromosome, complete genome.
ACCESSION   NZ_CP045096
VERSION     NZ_CP045096.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces phaeolivaceus
  ORGANISM  Streptomyces phaeolivaceus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10116136)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain GY16 isolated from leaves of Broussonetia
            papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10116136)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-AUG-2019
            Assembly Method                   :: HGAP v. v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009184865.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 02:51:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,113
            CDSs (total)                      :: 9,024
            Genes (coding)                    :: 8,642
            CDSs (with protein)               :: 8,642
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 112 of 382
            Pseudo Genes (incomplete)         :: 305 of 382
            Pseudo Genes (internal stop)      :: 23 of 382
            Pseudo Genes (multiple problems)  :: 53 of 382
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 325739
            Orig. end                         :: 336150
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045096.1.
            Bacteria and source DNA available from Yunlin Zhao.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10411
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10411
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10411
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5411](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5411
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    46..61
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 26.14"
                     /tool="antismash"
     gene            285..1199
                     /locus_tag="F9278_RS01845"
                     /old_locus_tag="F9278_01850"
     CDS             285..1199
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474266.1"
                     /locus_tag="F9278_RS01845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01850"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_152166677.1"
                     /transl_table=11
                     /translation="MMSELPLDLVRTLLAVVDEGTFDAAAGALHVTPSAVSQRVKALEQ
                     RVGRVLLIREKPVRATESGEVIVRFARQLARLERDAHTALGLTGAGQTPTTRVSIAVNA
                     DSLATWFLPALTRVPEDLRPCYELLREDEQHTARLLREGLVMAAVTSAPDAVAGCSVRP
                     LGRLRYLPCATPAFAERWLGAGSGVPLEDVIADAPVVFFDRRDDLQNAFVRRLTRGRPA
                     GVRRHHVPTSEGFVEAVAAGMGWGMVPAAQAERLLDAGRLVDLAPERGVDAPLFWQQWK
                     LDSPALTAVAEAVAAEAAEALDP"
     gene            complement(1239..1931)
                     /locus_tag="F9278_RS01850"
                     /old_locus_tag="F9278_01855"
     CDS             complement(1239..1931)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005956.1"
                     /locus_tag="F9278_RS01850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01855"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_152166678.1"
                     /transl_table=11
                     /translation="MTNKTVGTVGVDLPDRRGRTGLDRTGRELTGNPRVKVRDVQLLSS
                     NWYVERTTTFDFQHADGTWSTQERETHDRGNGATVLLYDTDRETVLLTRQFRYPVYVNG
                     HPDGMLIETPGGLLDEEDEHPEVAVRREVVEETGHTIGEIQHVFDIYMSPGSVTERVSF
                     YAAPYGPSTRTHEGGGLDEEGEDIELVELPFRRALEMIRGGEIADAKTIMLLQWAALEG
                     PFAAGGGA"
     gene            2035..2712
                     /locus_tag="F9278_RS01855"
                     /old_locus_tag="F9278_01860"
     CDS             2035..2712
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433207.1"
                     /locus_tag="F9278_RS01855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01860"
                     /product="AIM24 family protein"
                     /protein_id="WP_152166679.1"
                     /transl_table=11
                     /translation="MKSDLFSNENMVQPAYAPGMSVQNAKSIKYAVNGEMLARQGAMIA
                     FRGNLQFERKGQGVGGMLKRAVTGEGLPLMAVRGQGEAWFAHEAQNCFIVDIEPGDVFT
                     VNGRNVLCFDASLAYEIKTVKGAGMTGGGLFNSVFTGQGRLGLVCDGTPLVIPVSPQLP
                     VFVDTDAVVGWTAHLSTSLHRSQSFGSMIRGGSGEAVQLKLEGEGFVVVRPSEVTPQKA
                     QQH"
     misc_feature    complement(2771..2786)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.52"
                     /tool="antismash"
     misc_feature    2772..2785
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.71"
                     /tool="antismash"
     gene            2808..3434
                     /locus_tag="F9278_RS01860"
                     /old_locus_tag="F9278_01865"
     CDS             2808..3434
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007446634.1"
                     /locus_tag="F9278_RS01860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01865"
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="WP_152166680.1"
                     /transl_table=11
                     /translation="MATHLQGSLFDQSDDLRLGPLDGLRRRELGAGAWVDVLPGWLGGA
                     DTLFTRLAEEVPWKAERRQMYERTVSVPRLLAFYGAEDALPDPVLDEARQALSAHYGAE
                     LGEPFATAGLCHYRDGRDSVAWHGDRIGRGAREDTMVAILSVGDPRDLALRPHGGGRTL
                     RFPQGHGDLIVMGGSCQRTWDHAVPKSARAVGPRISIQFRPRGVR"
     gene            complement(3451..4662)
                     /locus_tag="F9278_RS01865"
                     /old_locus_tag="F9278_01870"
     CDS             complement(3451..4662)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009308697.1"
                     /locus_tag="F9278_RS01865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01870"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_193241338.1"
                     /transl_table=11
                     /translation="MKIACVGGGPASLYFSILMKRQDPSHDITVHERNPAGSTYGWGVT
                     YWSELLDKLREKDPETALAISENSVNWNSGVAYVRDRTTVQPGDDGFGIGRRRMLELLA
                     ERARALGVRVEYEDEITADDLPEADLVVAGDGVHSAVRERHADRFGSDITLGRNVYGWL
                     GTTKVYDAFTFSFQETDHGWIWAYAYPFSDEQSTCVVECSPRTLSGLGLDRMGEADGLA
                     LLEKLFADILDGHPLLGRAQDDDTAQWLTFRTLTNRSWRHGNLVLLGDAAHTTHYSIGA
                     GTTLALEDAICLAEALGAHPDTETALAAYERRRKSELLRLQSAARHSAQWYENIHRYID
                     LPPEQMFALLGQRHSPLLPYVPPQVYYRLDRAVGRLEALRRLKRWLGPKLARSSQSRAL
                     AGRK"
     misc_feature    complement(4950..4964)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            5001..5411
                     /locus_tag="F9278_RS01870"
                     /old_locus_tag="F9278_01875"
     CDS             5001..5411
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474271.1"
                     /locus_tag="F9278_RS01870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01875"
                     /product="apotyrosinase chaperone MelC1"
                     /protein_id="WP_152166682.1"
                     /sec_met_domain="melC (E-value: 2.6e-47, bitscore: 159.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLTRRHALGAAAALAVTVGTPLTAAVADDHADHADHADHADHD
                     GDAGHGGRQAPRAFDEVYRGRRIQGHATEGGGHHHGAGYAVFVDGAELHVMRNADGSWI
                     SVVSHYAPVSTPRAAARAAVDELQGARLVPFG"
     gene            5474..6298
                     /locus_tag="F9278_RS01875"
                     /old_locus_tag="F9278_01880"
     CDS             5474..6298
                     /EC_number="1.14.18.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662973.1"
                     /locus_tag="F9278_RS01875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01880"
                     /product="tyrosinase MelC2"
                     /protein_id="WP_152166683.1"
                     /transl_table=11
                     /translation="MTVRKNQATLTADEKRRFVDALIALKRSGRYDEFVTTHNAFILGD
                     TDNGERTGHRSPSFLPWHRRFLLEFERALQSVDPTVALPYWDWTADRTARSSLWAPDFL
                     GGTGRGRDGQVMDGPFAASTGDWPINVRIDGRTYLRRSLGGAVRQLPTRAEVDSVLAMS
                     TYDMAPWNSASDGFRNHLEGWRGVNLHNRVHVWVGGQMGTGVSPNDPVFWLHHAFVDRL
                     WADWRRLHPGSAYLPGAGTPNVVDLDETMKPWNDSTPAALLDHTAHYTFDAA"
     gene            complement(6306..7049)
                     /locus_tag="F9278_RS01880"
                     /old_locus_tag="F9278_01885"
     CDS             complement(6306..7049)
                     /EC_number="3.1.26.4"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009308694.1"
                     /locus_tag="F9278_RS01880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01885"
                     /product="ribonuclease H"
                     /protein_id="WP_152166684.1"
                     /transl_table=11
                     /translation="MIERMPERVVAACDGASKGNPGPAGWAWVVAENDEIPSRWEAGPL
                     GRATNNIAELTALERLLAATDPAVPIEIRMDSQYAMKAVTTWLPGWKRNGWRTAAGKPV
                     ANQELVVRIDELLADRAVEFRYVPAHQVDGDPLNDFADRAASQAAVVQEPAGTELGSPE
                     PPAAPDTVPSRWSAAKKSAKSGKPGRSGTRNGSSSSSSASSRTIKAKFPGRCLCGRPYA
                     AGEQIAKNDQGWGHPECRTATAGTA"
     gene            complement(7095..7904)
                     /locus_tag="F9278_RS01885"
                     /old_locus_tag="F9278_01890"
     CDS             complement(7095..7904)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982578.1"
                     /locus_tag="F9278_RS01885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01890"
                     /product="VOC family protein"
                     /protein_id="WP_152166685.1"
                     /transl_table=11
                     /translation="MAAHPEGTPCWADAMFSDVEAAKSFYGDVLGWTFGESSTEYGNYT
                     QAYVDGKAVAAVVPPMPGQEGQSAWCLYFASPDVNATAAKVRDNGGEVLMEPMRVGEFG
                     SMLLARSPDGVVFGVWQAGVHEGFEAMGVPGAYIWAEVFTREPEKSDTFFPAVFPFRAK
                     RMEDPDSPHMDFRVFHLGADPLLGRMRMTAEDFPPEVPSFINVYFTVADCDDAVAKATK
                     LGGILRYGPMDTPFGRFAALSDPQGASFSVIDVTNAQGEMPPMTDVD"
     misc_feature    8067..8081
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.47"
                     /tool="antismash"
     misc_feature    complement(8080..8098)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.44"
                     /tool="antismash"
     misc_feature    8092..8107
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.44"
                     /tool="antismash"
     gene            8129..8551
                     /locus_tag="F9278_RS01890"
                     /old_locus_tag="F9278_01895"
     CDS             8129..8551
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474280.1"
                     /locus_tag="F9278_RS01890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01895"
                     /product="DUF6153 family protein"
                     /protein_id="WP_152166686.1"
                     /transl_table=11
                     /translation="MSGRPFRRTAPPCARGRSARLMLVVSLVLTTFVLFCAGAPPGEAP
                     APRPHSVSADLPAERPDSARAGAVERDPCEHSPGGHDCHAADPAAVLGQAPLPGADHTA
                     ASWQPASAPAPVASFGSGEPGRARPPDLHELQLLRV"
     misc_feature    complement(8172..8186)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            8645..9322
                     /locus_tag="F9278_RS01895"
                     /old_locus_tag="F9278_01900"
     CDS             8645..9322
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474282.1"
                     /locus_tag="F9278_RS01895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01900"
                     /product="DUF3105 domain-containing protein"
                     /protein_id="WP_152166687.1"
                     /transl_table=11
                     /translation="MASSKSKAKNGGPTGKNSSPKAREAARRARVEEIRKAEQSRERRM
                     RLLTLGVTGVILAGLVGGGWFLIDSAQEKEEAKAAPVDGVKSWSKLSQNHVAEPVEYKM
                     SPPVGGDHNQVWVNCDKQVYTKVVPNENAVHALEHGAVWITYSDKAAKADVESLTDLVN
                     KTTYTFMSPYEDQSSPIVLSAWEHQLKVDKASDPRVQKFLDKYVQGEQTPEPGAACSGG
                     MTP"
     gene            9462..10391
                     /locus_tag="F9278_RS01900"
                     /old_locus_tag="F9278_01905"
     CDS             9462..10391
                     /EC_number="3.1.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987929.1"
                     /locus_tag="F9278_RS01900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F9278_01905"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_152166688.1"
                     /transl_table=11
                     /translation="MRKPWIVGVVGAVLTALLLGGCGDPASGPDEAPVSAAAPDPSSTT
                     RRRAATASPAEEGDPVKPAPKVLYLGDSLATENQNVLGSFLKDDLAARYTSAPYSGTTL
                     CDYLEGTADRSLVPPQDKAAALVRRLGPDYVVLQFWGNSWDYTPCMDGVTFGEDRAEYF
                     RRYTAAADLLTEQIADAGGTHRPRIVWVLQGPDPMTPDRIRRVNALYQKQAKASGDLVA
                     DAGKAVSPAADRYTWAQYLPCTAYERAHDGYCTQPGRARTALHHDEDFLHFCLAPTTSK
                     PRPCPVPSPGITRMAREITRAIADASGA"
ORIGIN
        1 ctcagcgcgc cgggccgggc gacgaccacc gcgccgaccc cggccacgcc gagggcgatc
       61 agcagcgcgt cggagagggc gcagatcgcg acgaccggga ggacggcgtc gcggtgcagc
      121 ccctggcgca ggacgaaggc gttctgggcg ccgatggcga cgatcaggga caggccggtg
      181 ccgaatccgg cggcgagggc ggtcatttcg ctgaacacac ccccgacgct aggaaaagcg
      241 gcgtcatgcg tacagctaaa gattcttacg tatcattagc gctcatgatg tccgagctgc
      301 cgttggatct ggtgcgcacg ctgctcgcgg tggtggacga gggcacgttc gacgcggcgg
      361 ccggtgccct gcatgtgacg ccgtcggcgg tcagccagcg ggtcaaggcg ctggagcagc
      421 gggtcggccg ggtgctgctg atccgggaga agccggtgcg ggcgaccgag tccggcgagg
      481 tgatcgtgcg gttcgcgcgg cagctggccc gtctcgaacg cgacgcgcac accgcgctcg
      541 gcctgaccgg cgccgggcag accccgacca cgcgggtgtc gatcgcggtg aacgcggact
      601 cgctggccac ctggttcctg cccgccctga cccgggtgcc cgaggacctg cgcccctgct
      661 acgagctgct ccgcgaggac gagcagcaca cggcccggct gctgcgcgag gggctggtga
      721 tggccgcggt cacctcggcg ccggacgccg tggccggctg ctcggtccgg ccgctcgggc
      781 ggctgcggta cctgccctgt gcgacgcccg cgttcgccga gcggtggctc ggcgccggtt
      841 cgggtgtgcc actggaggac gtgatcgcgg acgcgccggt cgtgttcttc gaccggcggg
      901 acgacctcca gaacgcgttc gtccgccggc tgacgcgggg ccgcccggcc ggtgtgcgac
      961 ggcatcatgt gccgacctcg gagggcttcg tggaggccgt ggccgccggg atgggctggg
     1021 gcatggtgcc cgccgcgcag gccgagcggc tgctggacgc cgggcggctc gtcgatctgg
     1081 cgccggagcg gggtgtggac gccccgctgt tctggcagca gtggaagctc gactcccccg
     1141 cgctgacggc cgtggcggag gcggtcgcgg cggaggccgc cgaggcgctg gacccctgag
     1201 gccgaggccc gcgcctcccg cctcccccgc cgcccgcgtc aggcgcctcc cccggcggcg
     1261 aacggcccct ccagcgcggc ccattggagc agcatgatgg tcttggcgtc ggcgatctcc
     1321 ccgccgcgga tcatctccag ggcccggcgg aagggcagtt cgacgagttc gatgtcctcg
     1381 ccctcctcgt cgaggccgcc gccctcgtgg gtgcgggtcg acgggccgta gggggcggcg
     1441 tagaagctga cgcgttcggt gaccgagccg gggctcatat agatgtcgaa gacgtgctgg
     1501 atctcgccga tggtgtggcc ggtctcctcg acgacctcgc gccgcacggc gacctcgggg
     1561 tgctcgtcct cctcgtcgag caggccgccc ggcgtctcga tcagcatccc gtccgggtgg
     1621 ccgttgacgt acaccgggta gcggaactgc cgggtgagca gcacggtttc gcggtcggtg
     1681 tcgtagagga ggacggtggc gccgttgccc cggtcgtgcg tctcgcgttc ctgggtgctc
     1741 caggtgccgt cggcgtgctg gaagtcgaag gtggtggtgc gctccacgta ccagttgctg
     1801 gacagcagtt ggacgtcccg taccttcacg cgcgggttgc cggtgagttc ccggccggtg
     1861 cggtcgagtc cggtacggcc ccggcggtcc gggaggtcga caccgacggt gccgacggtc
     1921 ttgttggtca tgtgtccgct tctaccattc cgatcatggc gttgtcaggc gcctcaaatg
     1981 gcgcaccgcg cgcgtcgctt ggcggaagcg ggcactccgg gacagcatgc ccgtatgaag
     2041 agcgatcttt tctccaacga gaacatggtc cagccggcgt acgcgccggg gatgagcgtc
     2101 cagaacgcca agtcgatcaa gtacgcggtc aacggcgaga tgctcgcccg gcagggcgcg
     2161 atgatcgcct tccgggggaa cctccagttc gaacgcaagg gccagggcgt gggcggcatg
     2221 ctcaagcgcg cggtcaccgg ggaggggctg ccgctgatgg cggtgcgcgg ccagggcgag
     2281 gcctggttcg cgcacgaggc gcagaactgc ttcatcgtcg acatcgagcc gggtgatgtg
     2341 ttcaccgtca acggccgcaa cgtgctctgt ttcgacgcct cgctggcgta cgagatcaag
     2401 accgtgaagg gcgccgggat gaccggcggc ggcctgttca acagtgtgtt caccgggcag
     2461 ggcaggctcg gtctcgtctg tgacggcact ccgctggtca tcccggtctc gccgcagctg
     2521 ccggtgttcg tggacacgga cgcggtcgtc ggctggacgg cccacctcag cacctcgctg
     2581 caccgctccc agtccttcgg ctcgatgatc cggggcggct ccggcgaggc cgtgcagctg
     2641 aagctggagg gcgagggctt cgtggtggtc cggccgagcg aggtgacccc gcagaaggcc
     2701 cagcagcact gagccgccgg gcgacgaggg ggcccggtgg gcgaccgccg gtgagcgact
     2761 ccacccacta acgaacagat tttcgaatcg cggagtacgc tgcgggtatg gccacgcacc
     2821 tccagggctc cctcttcgac cagtccgacg acctccgcct cggccccctc gacggcctgc
     2881 gtcggcgtga gctgggcgcc ggggcctggg tcgacgtcct gcccgggtgg ctcggcgggg
     2941 ccgacacgct gttcacccgg ctcgccgaag aggtgccctg gaaggcggag cgccggcaga
     3001 tgtacgagcg gacggtgtcc gtaccgcgtc tgctcgcctt ctacggcgcc gaggacgccc
     3061 tgccggatcc cgtcctggac gaggcccggc aggcgctctc cgcgcactac ggcgccgagc
     3121 tgggcgagcc gttcgccacg gcggggctct gccactaccg cgacggccgg gacagcgtgg
     3181 cctggcacgg cgaccggatc gggcggggcg cccgggagga cacgatggtg gcgatcctgt
     3241 ccgtgggcga cccccgcgat ctggccctgc gcccgcacgg cggcgggcgg acactgcggt
     3301 tccctcaggg gcacggcgac ctgatcgtga tgggcggctc ctgccagcgc acctgggacc
     3361 acgcggttcc caagtcggcg cgggcggtgg ggccgcgcat cagcatccag ttccggccac
     3421 ggggcgtgcg gtagcggcgc ccggggtccg ctacttccgg ccggcgaggg cgcgcgactg
     3481 ggacgagcgg gccagcttcg ggcccagcca gcgcttgagg cggcgcagcg cctccagacg
     3541 gccgaccgcg cggtcgaggc ggtagtagac ctgcggcggc acatagggca gcagcgggga
     3601 gtggcgctgg ccgaggagcg cgaacatctg ctcgggcggc aggtcgatgt accggtggat
     3661 gttctcgtac cactgggcgc tgtggcgggc cgcgctctgc agccgcagca gctcggactt
     3721 gcgccggcgc tcgtacgcgg cgagcgcggt ctccgtgtcc ggatgggcgc cgagggcctc
     3781 ggccaggcag atcgcgtcct ccagggcgag ggtggtgccc gcgccgatgg agtagtgcgt
     3841 ggtgtgggcg gcgtcgccga gcaggaccag attgccgtgc cgccaggagc ggttggtcag
     3901 ggtgcggaag gtgagccact gggcggtgtc gtcgtcctgg gcacgaccca ggagcgggtg
     3961 gccgtccagg atgtcggcga acagcttctc cagcagggcc aggccgtccg cctcgcccat
     4021 gcggtccagg cccaggccgc tcagcgtcct cggggagcac tcgacgacgc aggtgctctg
     4081 ctcgtcgctg aacgggtagg cgtaggccca gatccagccg tggtcggtct cctggaagga
     4141 gaaggtgaag gcgtcgtaga ccttggtggt gcccagccag ccgtagacgt tgcggccgag
     4201 ggtgatgtcg ctgccgaagc ggtcggcgtg ccgctcgcgc accgcgctgt ggacgccgtc
     4261 gcccgcgacg acgagatcgg cctcgggcag gtcgtcggcg gtgatctcgt cctcgtactc
     4321 cacgcggacc ccgagggccc gcgcccgctc ggcgagcagc tccagcatgc gacgccggcc
     4381 gatgccgaaa ccgtcgtcgc cgggctggac ggtcgtacgg tcgcggacgt acgccacgcc
     4441 gctgttccag ttgacggagt tctcgctgat ggcgagggcc gtctcggggt ccttctcgcg
     4501 gagcttgtcc agcagttcgg accagtaggt gacaccccag ccgtaggtcg atccggccgg
     4561 gttccgttca tggacggtga tgtcgtggga cgggtcctgc cgcttcatca ggatcgagaa
     4621 gtacaggctc gccggtccgc caccgacgca cgcgatcttc acgcgaactc ccatttcgct
     4681 gcgcaaagcg atcaattgac cacgaagagt agcagtgcgc gcgcactccg gattcacgtc
     4741 actttgcccg cgtgatcccg gccacgcgat ccgcgccgac accgcaagat catcggcgcc
     4801 accgccgcca ccgggcggaa tatctccctc caccctcgcc ccggaggccg tggcagcaag
     4861 atcatcctta ttcaaccttg cacgacattt acttaattgt ccggatcgga gccaaccgac
     4921 tccctgggat ttcccccgct cctccatgga tcgataggca tgacgggtca tcaacccacc
     4981 ctttcccagg aggccttcgc atgcccgacc tcacccgccg ccacgcgctc ggcgccgcgg
     5041 cagccctcgc cgtgaccgtc ggcaccccgc tcaccgccgc ggtcgcggac gaccacgcag
     5101 accacgcaga ccacgcagac cacgcagacc acgacggcga cgccggtcat ggcggccgac
     5161 aggccccccg ggccttcgac gaggtctacc ggggccgtcg gatacagggg cacgcgaccg
     5221 agggcggcgg tcaccatcac ggcgccgggt acgccgtgtt cgtcgacggc gccgaactgc
     5281 atgtgatgcg gaacgccgac ggcagctgga tcagcgtcgt cagccactac gccccggtgt
     5341 cgaccccgcg cgccgccgcc cgcgccgcgg tcgacgaact ccagggcgcc cgtctcgtcc
     5401 ccttcggctg atccccaccc gcaccacccg caccaccgga tccacccgaa cgaccgcgag
     5461 gagccgcagc accatgaccg tacgcaagaa ccaggccacc ctgaccgccg acgagaaacg
     5521 gcgcttcgtc gacgcgctga tcgcgctcaa gcgctcgggc cgctacgacg agttcgtcac
     5581 gacccacaac gcgttcatcc tcggcgacac cgacaacggt gaacgcacgg gccaccgttc
     5641 gccgtccttc ctgccctggc accgcagatt cctgctggag ttcgaacggg ccctgcagtc
     5701 cgtggacccg accgtcgcgc tcccctactg ggactggacg gccgaccgca cggcccgctc
     5761 gtcactgtgg gcgccggact tcctcggcgg caccgggcgc ggccgcgacg ggcaggtgat
     5821 ggacggcccg ttcgccgcgt ccaccggtga ctggccgatc aacgtacgca tcgacggccg
     5881 cacctatctg cggcgctccc tcgggggcgc ggtacggcag ttgccgaccc gggccgaggt
     5941 ggactcggtg ctcgcgatgt ccacgtacga catggcgccc tggaacagtg cctcggacgg
     6001 tttccgcaat catctggagg gctggcgggg tgtgaacctg cacaacaggg tgcatgtctg
     6061 ggtgggcggt cagatgggca ccggggtctc ccccaacgac ccggtcttct ggctgcacca
     6121 cgcgttcgtc gaccggctgt gggccgactg gcggcgcctg cacccgggct cggcgtatct
     6181 gccgggcgcc gggacaccga acgtggtcga cctcgacgag acgatgaagc cgtggaacga
     6241 ctcgaccccg gcggcgctgc tggaccacac cgcgcactac acgttcgacg cggcctgacc
     6301 cgcgctcacg ccgttccggc ggtcgccgtg cggcactccg ggtgccccca gccctggtcg
     6361 ttcttggcga tctgctcgcc cgcggcgtag ggacggccgc acaggcagcg gccgggaaac
     6421 ttcgccttga tggtgcggga cgaggccgag gacgacgagg acgagccgtt ccgggtgccg
     6481 gacctgccgg gcttcccgga cttcgcggac ttcttcgccg cgctccaacg ggacgggacc
     6541 gtgtcggggg cggccggggg ttccggggag cccagttcgg tgccggcggg ttcctggacg
     6601 acggccgcct ggctggccgc gcggtcggcg aagtcgttga gcgggtcgcc gtcgacctgg
     6661 tgggcgggga cgtagcggaa ctccacggcg cggtcggcga gcaactcgtc gatacggacc
     6721 acgagttcct ggttggcgac cggcttgccg gcggccgtgc gccagccgtt gcgcttccag
     6781 ccgggcagcc aggtggtgac cgccttcatc gcgtactggg agtccatccg gatctcgatc
     6841 gggacggccg ggtctgtggc cgccaacagc cgctccagcg ccgtgagttc ggcgatgttg
     6901 ttggtggctc tgcccagcgg gccggcctcc cagcgcgagg ggatctcgtc gttctcggcg
     6961 accacccagg cccagcccgc tggtcccggg tttcccttgg acgctccgtc acaggcggcc
     7021 acaacacgtt ccggcatgcg ctcgatcatg ccatgcgcgg gggtgtggga ccgtccacgg
     7081 gtcacacacc ccgctcagtc cacgtccgtc atcggcggca tctcgccctg ggcgttggtg
     7141 acgtcgatga cggagaacga ggcgccctgc gggtcgctga gcgcggcgaa gcggccgaag
     7201 ggggtgtcca tcgggccgta gcgcaggatg ccgccgagct tggtggcctt ggcgaccgcg
     7261 tcgtcgcagt cggcgacggt gaagtacacg ttgatgaacg agggcacctc gggcgggaag
     7321 tcctccgccg tcatccgcat ccggccgagg agcgggtccg cccccaggtg gaagacccgg
     7381 aagtccatgt gcgggctgtc gggatcctcc atccgcttgg cccggaacgg gaagacggcc
     7441 gggaagaacg tgtccgactt ctcgggctcg cgggtgaaga cctcggccca gatgtacgca
     7501 ccgggcacgc ccatggcctc gaagccctcg tgaactccgg cctgccagac gccgaagacg
     7561 acaccgtccg gggagcgggc gagcagcatg gagccgaact cgccgacccg catcggctcc
     7621 atcagcacct cgccgccgtt gtcgcggacc ttggcggcgg tggcgttcac atcgggcgag
     7681 gcgaagtaca ggcaccaggc ggactgtccc tcctggccgg gcatcggcgg gacgaccgcg
     7741 gccaccgcct tgccgtccac gtacgcctgg gtgtagttgc cgtactcggt ggacgactcg
     7801 ccgaacgtcc agcccagtac gtcgccgtag aaactcttcg ccgcctcgac atcgctgaac
     7861 atcgcgtcgg cccaacaggg cgttccctcg ggatgtgcgg ccatgactgc cactctcctc
     7921 ggttcgactg gtggttcccc cggacaggtg gcctccccgg tctcacgcta gccaggaccg
     7981 ctcccggacg cgcgccgaac gggacggacc gcgtgaaacc ggcaggggat ccagaggacg
     8041 ggggcacgtt cgggaccacg gctgtgcgtc gtctgataat ttcagcgacg ctcgcactcc
     8101 cgttcgcctc gactcgtgag gtgccaccat gtccggccgg ccgttcagac gtacggcgcc
     8161 gccgtgcgcc cggggccggt cggcgcgtct gatgctcgtg gtctcgctgg tcctcaccac
     8221 gttcgtgttg ttctgcgcgg gtgcgccgcc cggtgaggct ccggcgccgc gcccgcactc
     8281 ggtgtcggcg gatctcccgg cggagcggcc ggacagcgcg cgggcgggcg cggtggagcg
     8341 cgacccctgt gagcacagcc ccggcgggca cgactgtcat gccgccgacc cggcggcggt
     8401 gctgggccag gcgccgctgc ccggcgccga tcacaccgcc gcgtcctggc agcccgcgtc
     8461 ggcaccggca ccggtcgcgt ccttcggctc cggggagccg gggcgggccc gtcctcccga
     8521 tctccacgaa ctgcagttgc tccgcgtcta ggccggaccc ggcccgtacg acgcgcacat
     8581 acgactcgca tcgcaggacc gccgtgcccg cgcgggcacg gcagggagca gaaggacgat
     8641 ccccatggct tcatccaagt cgaaggccaa gaacggcgga ccgacgggca agaactccag
     8701 ccccaaggcc agggaggcgg cgcggcgtgc ccgcgtcgag gagatacgca aggccgagca
     8761 gtcccgggag cggcgcatgc ggctgctcac gctcggcgtc accggtgtga tcctcgccgg
     8821 gctggtgggc ggcggctggt tcctgatcga ctccgcccag gagaaggagg aggcgaaggc
     8881 cgcgccggtc gacggggtga agagctggtc gaagctctcc cagaaccatg tcgccgagcc
     8941 cgtcgagtac aagatgagcc cgcccgtcgg cggcgaccac aaccaggtgt gggtcaactg
     9001 cgacaagcag gtgtacacca aggtcgtgcc gaacgagaac gcggtgcacg cgctggagca
     9061 cggcgccgtc tggatcacgt acagcgacaa ggcggccaag gcggatgtcg agtcgctcac
     9121 cgatctcgtg aacaagacga cgtacacctt catgagcccc tacgaggacc agtcctcgcc
     9181 catcgtgctg agtgcctggg agcaccagtt gaaggtggac aaggcgtccg acccgcgggt
     9241 gcagaagttc ctcgacaagt acgtgcaggg cgagcagacg ccggagccgg gtgccgcgtg
     9301 ctccggcggg atgaccccgt gagcggcggc acctcctgag cggagagggc gtccggtcgt
     9361 ttccgttccg ggcgccctcg tggccgacac gggtcttccg ccccgccccg ggctgtgctt
     9421 gcctggggag ccttttcgat ggccggggcg ggagtcgccg tatgcgcaag ccgtggatcg
     9481 tgggagtggt gggggcggtg ctcaccgcgc tgctgctcgg cgggtgcggg gatccggcgt
     9541 ccgggccgga cgaggccccg gtgtcggccg cggctccgga cccgtcgtcg accacccgcc
     9601 gaagggccgc cacggcgtcc cccgccgaag agggcgaccc ggtgaagccg gcgccgaagg
     9661 tgctctacct cggtgactcg ctcgcgacgg agaaccagaa cgtcctgggc tccttcctga
     9721 aggacgatct ggccgcccgc tacaccagcg ccccgtactc gggcaccacc ctgtgcgact
     9781 acctggaggg caccgccgac cggtcgctgg tgccgccgca ggacaaggcc gccgcgctgg
     9841 tgcggcggct gggcccggac tatgtggtgc tgcagttctg gggcaactcc tgggactaca
     9901 cgccctgcat ggacggtgtc accttcggcg aggaccgtgc cgagtacttc cggcggtaca
     9961 cggccgccgc cgatctgctc accgagcaga tcgcggacgc gggcggcacg caccggccga
    10021 ggatcgtatg ggtgttgcag ggcccggacc cgatgacccc ggaccggatc cgccgggtga
    10081 acgccctgta ccagaagcag gccaaggcct ccggtgatct ggtcgccgac gccggcaagg
    10141 ccgtgagccc ggccgccgac cgctacacct gggcccagta cctgccgtgc acggcgtacg
    10201 agcgcgcgca cgacggctac tgcacccagc ccggccgggc ccgcaccgcc ctccatcacg
    10261 acgaggactt cctgcacttc tgtctggcgc cgacgacgtc caagccgcgg ccgtgcccgg
    10321 tgccgtcccc ggggatcacg cggatggccc gggagatcac ccgggccatc gccgacgcga
    10381 gcggtgccta gacccgctgc gaggtggtgg g
//
