LOCUS       NZ_WBPF01000005        10273 bp    DNA     linear   CON 16-NOV-2023
DEFINITION  Bacillus sp. CH140a_4T NODE_5_length_1064852_cov_68.882179, whole
            genome shotgun sequence.
ACCESSION   NZ_WBPF01000005
VERSION     NZ_WBPF01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. CH140a_4T
  ORGANISM  Bacillus sp. CH140a_4T
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1064950)
  AUTHORS   Olmedo-Alvarez,G., Saldana,S. and Barcelo,D.
  TITLE     Bacillus from the desert of Cuatro Cinegas, Coahuila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1064950)
  AUTHORS   Olmedo-Alvarez,G., Saldana,S. and Barcelo,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2019) Ingenieria Genetica, Cinvestav, Libramiento
            Norte Carretera Irapuato Leon Kilometro 9.6, Carr Panamericana
            Irapuato Leon, Irapuato 36824, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2013
            Assembly Method                   :: A5-miseq v. 2013
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/16/2023 02:51:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,545
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 108
            rRNAs                             :: 8, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 23S)
            partial rRNAs                     :: 4, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 78 of 187
            Pseudo Genes (incomplete)         :: 114 of 187
            Pseudo Genes (internal stop)      :: 56 of 187
            Pseudo Genes (multiple problems)  :: 47 of 187
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:06:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 77034
            Orig. end                         :: 87307
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WBPF01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10273
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10273
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10273
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5273](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5273
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(77..538)
                     /locus_tag="F8162_RS01335"
                     /old_locus_tag="F8162_01335"
     CDS             complement(77..538)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001982483.1"
                     /locus_tag="F8162_RS01335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01335"
                     /product="DUF2306 domain-containing protein"
                     /protein_id="WP_151582821.1"
                     /transl_table=11
                     /translation="MSIFNILLTIHILFGTICLTTGIIAMVAQKKKGKHTEWGEIYHAS
                     YVVITLTAIILSIINWDKIAYLFYVAIFSYSFAIYGYLARKKRWKNWLHHHIIGMLGSY
                     IGAVTALLVNIGIHIPLINLLPPIWFWFLPTLIGIPLVASVSKKYKKRS"
     gene            713..1243
                     /locus_tag="F8162_RS01340"
                     /old_locus_tag="F8162_01340"
     CDS             713..1243
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495179.1"
                     /locus_tag="F8162_RS01340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01340"
                     /product="DUF4269 domain-containing protein"
                     /protein_id="WP_151582823.1"
                     /transl_table=11
                     /translation="MFTSITYLQSGNDKQQKVYDVLNKLNIMEDLALYSPVLCGTIPIR
                     IDTLQSDLDIVMEVHNFDVFEQEMRSLYGSYKGFKIKKKKIKNTESIKVNFEFEGFDFE
                     LFAQPKPVRNQNAYRHMIVEHMLLMQHPHLRKEILHLKENGLKTEPAFAQVLDIKGDPY
                     EELILLGQEMRLW"
     gene            complement(1289..2722)
                     /locus_tag="F8162_RS01345"
                     /old_locus_tag="F8162_01345"
     CDS             complement(1289..2722)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (117-466). E-value:
                     2.4e-36. Score: 117.5. Matches aSDomain:
                     nrpspksdomains_F8162_RS01345_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213549.1"
                     /locus_tag="F8162_RS01345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01345"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_151582825.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.6e-35, bitscore:
                     117.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTNKKLPKYRQIIDYMKEKIENGEWPIGSKIPSQRQLAKLFHVN
                     RSTVITALDELMADGLIQGKIGAGTIVTNNTWSLLATNPPPDWSEYVKAGIHKPSKLMV
                     REINEAEANKELIHLSKGELAPQVFPLETMQSIMKSICEELDYFGYEEQKGFLPLREAI
                     SKYVKSFGIHASASSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQSANI
                     NLAGICMDHHGILPNELLKRIKYSQKKNILYSIPCFQNPTGILMSLERRKEILKICEKE
                     QLPIIEDDIYRELWIDELPPAPLKSIDKHGHVLYVGSLSKTLTPGLRIGWIIGPEPVID
                     RLSDIKMQTDYGSSSLSQRVAAEWINKGFYEEHVANIRIQLKERRQIMIRALNKYCADI
                     ATWDIPSGGFFIWLKVVPSIPMKKLFSEALSKGILLNPGRIYEEESDQYIRLSYGYASP
                     EQMTNGIELLGELIRKLMT"
     aSDomain        complement(1325..2371)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F8162_RS01345_Aminotran_1_2.1"
                     /evalue="2.40E-36"
                     /label="F8162_RS01345_Aminotran_1_2.1"
                     /locus_tag="F8162_RS01345"
                     /protein_end="466"
                     /protein_start="117"
                     /score="117.5"
                     /tool="antismash"
                     /translation="HLSKGELAPQVFPLETMQSIMKSICEELDYFGYEEQKGFLPLREA
                     ISKYVKSFGIHASASSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQSAN
                     INLAGICMDHHGILPNELLKRIKYSQKKNILYSIPCFQNPTGILMSLERRKEILKICEK
                     EQLPIIEDDIYRELWIDELPPAPLKSIDKHGHVLYVGSLSKTLTPGLRIGWIIGPEPVI
                     DRLSDIKMQTDYGSSSLSQRVAAEWINKGFYEEHVANIRIQLKERRQIMIRALNKYCAD
                     IATWDIPSGGFFIWLKVVPSIPMKKLFSEALSKGILLNPGRIYEEESDQYIRLSYGYAS
                     PEQMTNGIE"
     gene            2874..3729
                     /locus_tag="F8162_RS01350"
                     /old_locus_tag="F8162_01350"
                     /pseudo=""
     CDS             2874..3729
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551598.1"
                     /locus_tag="F8162_RS01350"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01350"
                     /product="DMT family transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHNKKWDLRIICAHILYTRASYFTTFINCFIYTTFVFFYI"
     gene            3863..4330
                     /locus_tag="F8162_RS01355"
                     /old_locus_tag="F8162_01355"
     CDS             3863..4330
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098590.1"
                     /locus_tag="F8162_RS01355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01355"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_151582827.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEDALKLQVKENQMKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLIVGFIMHAVVRETTNMYWINGFIIDKKQQGNGYGKAALQKNIYL
                     IKNTCKACKEIRLTVHKDNISAKKLYERYGFQSLGHEYDGEQVYRLFVNQI"
     gene            4356..4802
                     /locus_tag="F8162_RS01360"
                     /old_locus_tag="F8162_01360"
     CDS             4356..4802
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="F8162_RS01360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01360"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_151582829.1"
                     /transl_table=11
                     /translation="MKTKFHHIVRAVMIKDKKLLVAEYIGHHYFLPGGHVEVGESAENA
                     LIRELQEELGVNCSIKQFLGVIENQWEDREVLHHEINHMFEIDSQELHIDFTPNSKESH
                     LAFHWIDYNQEALNTYKIMPAPSVKELLERKLSDELLNCWISNF"
     gene            5001..5273
                     /locus_tag="F8162_RS01365"
                     /old_locus_tag="F8162_01365"
     CDS             5001..5273
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071378.1"
                     /locus_tag="F8162_RS01365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01365"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_151582831.1"
                     /sec_met_domain="TIGR03601 (E-value: 9.5e-44, bitscore:
                     143.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQLQQNQYPYYYIQDDARRCG
                     GCGGCGGRCGGCGGGRCGGCAGRCGGCIGCAGCFSCFNCWNWWII"
     gene            5376..5904
                     /locus_tag="F8162_RS01370"
                     /old_locus_tag="F8162_01370"
                     /pseudo=""
     CDS             5376..5904
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="F8162_RS01370"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01370"
                     /product="serine hydrolase domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEENHSSATALCIIKDNKID"
     gene            5936..6079
                     /locus_tag="F8162_RS28600"
     CDS             5936..6079
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="F8162_RS28600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653324.1"
                     /transl_table=11
                     /translation="MKDKINKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6369..7577
                     /locus_tag="F8162_RS01375"
                     /old_locus_tag="F8162_01375"
     CDS             6369..7577
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="F8162_RS01375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01375"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_151582834.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNA
                     VAITFSITSFSLSFSTLFAGKLQQKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGSLIDSLGVS
                     QAFLYWGIIVLLLVLTGSFFLREAVVSNTVTETLHSDYTSREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFIIIGFLSVISFVISILIRPPKAEKKKEIKHLHR
                     KVA"
     gene            7755..8198
                     /locus_tag="F8162_RS01380"
                     /old_locus_tag="F8162_01380"
     CDS             7755..8198
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="F8162_RS01380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01380"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_151582836.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKVLMVKNKGKKGSYYTLPGGAVKFGETL
                     EEAVIREVKEETGLHINVNGICYISEAFFEERGHHAIFFNFLGEIIGGETNITRPKEIE
                     EITWMELHRAAPHLRIPEHLLNTLQRKETVPYFFNGTIIHQSS"
     gene            8229..9359
                     /gene="dnaN"
                     /locus_tag="F8162_RS01385"
                     /old_locus_tag="F8162_01385"
     CDS             8229..9359
                     /EC_number="2.7.7.7"
                     /GO_component="GO:0009360 - DNA polymerase III complex
                     [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /gene="dnaN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394467.1"
                     /locus_tag="F8162_RS01385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01385"
                     /product="DNA polymerase III subunit beta"
                     /protein_id="WP_151582838.1"
                     /transl_table=11
                     /translation="MEFIVNHKHFTQALSEVSKAISTKAIIPILSGIKIIADQSGITLI
                     ASNSNIFIEKFIPNSNEDDQITTIVKAGTIVVPAKYFIEIIKKMPSDVSIKSTNEQIIT
                     IQSEEITLNLNGLPAYEFPNVPLIDDHAKVQVKTEQLIEVFKQTAFAVAKNESRPVLTG
                     VHIELDHNKLICAATDSHRLAIREMIISSDVKANCIVPSATISELLKLMSSNSKFVYIY
                     LSESHIIFTFGTTTLYSRLIEGKYPNISSLIPNDFQTVINIDRKRILQGVDRSSLLASE
                     WANNNVNLEIINESTIKISSNASQIGKISETQQIDTIQGEKQLNISFDGRFMLDALRAI
                     KEETVTLSFSGSMRPILIEAGTQSAAVHLISPVRAY"
     gene            9382..9894
                     /locus_tag="F8162_RS01390"
                     /old_locus_tag="F8162_01390"
     CDS             9382..9894
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276538.1"
                     /locus_tag="F8162_RS01390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F8162_01390"
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="WP_151582840.1"
                     /transl_table=11
                     /translation="MYKHTLCFIKRNEEILMLNREYDPVKGLWNGVGGKIEKGETPLDN
                     AIREIKEETNIEVKHDQIRFKGIIKWEDSSYSGGMYVYLVELLNEFTYHTPKKVSEGIL
                     DWKEISWILSDYNYGVGEMIPKFLYALLHNELILEHNFVLSNHKLIDYWNEELAKQDSS
                     IDNIVQL"
ORIGIN
        1 aatttattaa taaaatatta cattgagtat gatttttcat aataatcatc agaaagtaac
       61 gaaaatgtaa tgaattttaa ctacgctttt tatatttttt tgatacactg gctacgagag
      121 gaataccgat taatgtaggt aaaaaccaaa accatatagg tggcagtaaa ttaattaagg
      181 gaatatgaat accgatgttt accaaaagtg cggtaacagc accgatataa gagcctaaca
      241 tacctataat atggtggtga agccaatttt tccaccgttt ttttcgtgcg aggtatccat
      301 agatcgcaaa tgaataagag aaaattgcca cataaaataa atatgcgatt ttatcccagt
      361 ttataatgga taatataatt gcagtaaggg tgatgacaac gtaggatgca tgatatattt
      421 caccccattc cgtatgtttt cctttcttct tttgagcaac catcgctatt atcccagtgg
      481 ttaaacatat cgttccaaat aatatgtgga tggttaaaag aatgttgaaa atgctcataa
      541 taattcctcc ctttttattg ttatagtatt gcagtatagg agaaaagttg aaacatattg
      601 tttattcact ttaatatatt gaaataaagg tactccaaga acaagtttaa acggacaaat
      661 ttttcttgta tttagtgaga tacgtattac gtgaaatgaa ggagagactt ccatgtttac
      721 atctattaca tatttacaat cagggaatga caagcaacaa aaggtatatg atgttttgaa
      781 taaactaaac ataatggagg atttagcttt gtatagtccc gttctttgcg ggacaatccc
      841 tataagaatt gatactcttc aatctgactt agatatagta atggaagtac ataactttga
      901 tgtttttgaa caggaaatga gatctttata cggttcttat aaaggattca aaataaaaaa
      961 gaaaaaaatt aaaaataccg aatcgataaa ggtaaatttt gaatttgaag gttttgattt
     1021 tgaattattt gctcagccta agccagtacg taaccaaaat gcatatagac atatgatagt
     1081 agagcacatg ttattaatgc agcatcccca tctaaggaaa gaaattcttc acttaaaaga
     1141 gaacggttta aaaacagaac ctgcttttgc tcaagtatta gacattaaag gggatcctta
     1201 tgaagaactt attttattgg gacaagaaat gagattgtgg taaataagat acataaaaac
     1261 tcctctataa ataaaagagg agtttttgtt atgtcattaa cttacggatt aattcgccta
     1321 acaattcaat cccgttagtc atttgctctg gagaagcata tccatatgat aaacgaatgt
     1381 actgatctga ttcttcttca tagatacgtc ctgggtttaa aagaattcct ttggatagtg
     1441 cctctgaaaa taatttcttc atcggaatgc taggtacaac ctttagccat ataaaaaatc
     1501 caccactagg aatatcccag gtggcaatat ctgcacaata cttatttaaa gcacgtatca
     1561 ttatttgtct tcgttctttg agttgaattc ttatgtttgc aacatgttcc tcataaaatc
     1621 ctttatttat ccattcagca gcaactcttt gtgataaaga gctcgatcca tagtctgttt
     1681 gcatttttat atctgacaat ctatcaatta caggttctgg tccaataatc catccaattc
     1741 taagtccagg agtaagtgtt ttagagagac taccaacata taatacatgt ccatgtttat
     1801 ctatcgactt taaaggagct ggaggtagtt catcaatcca taattcgcgg taaatatcat
     1861 cttcaataat aggtaactgt tccttttcac atatttttaa tatttctttt cttcgctcta
     1921 gagacattaa tatccctgtt ggattttgaa aacaagggat agaatataaa atatttttct
     1981 tttgactata tttgattcgc tttaataact cgttaggtaa aataccgtga tgatccatac
     2041 atataccagc tagatttata tttgctgatt gaaatacatg aagtgaatat aagtaagaag
     2101 gttgctcaag taaaacggtt gattctctat gcaatagacc aattgatatt aattgcaatg
     2161 cttgtaacgc accagaaacg attaatatag aactagctga tgcatgtatt ccaaatgatt
     2221 ttacgtattt actaattgct tcacgtaacg gtaaaaaccc tttttgttct tcatatccaa
     2281 aataatctaa ttcctcacat atgcttttca taattgactg catagtttct agtgggaaaa
     2341 cttgaggcgc aagttcgcct ttactaaggt ggataagttc tttatttgct tctgcttcat
     2401 ttatttctcg taccattaac ttgctaggtt tatgaatacc agcctttaca tattcactcc
     2461 aatcaggcgg gggattcgtc gctaataatg accatgtatt atttgtcaca attgttcctg
     2521 ctccaatttt tccttgaatc aatccgtctg ccataagttc atctagtgca gttattacag
     2581 tacttcgatt tacatgaaat aatttggcta actgccgttg actagggatc ttactaccaa
     2641 taggccattc tccgttttcg attttttctt tcatataatc tattatctgc cgatatttag
     2701 gcagtttttt attagttgtc ataaaaaaac tccctcgttt actttaaaaa tgccaagtgg
     2761 ttggttttta cgataaatga ctggttgaag acattgtatc aactattaaa tatgatgaaa
     2821 agaaataatt ggtaatttta aagtggttgg tttattagga agggagagat aacatgcata
     2881 ataaaaaatg ggatttacgc atcatatgtg ctcacatctt atacaccaga gcatcttact
     2941 ttactacgtt tattaattgc ttcatttata ctacttttgt tttcttttat atataagtta
     3001 cggctaccaa atttaaagga tatcccagca atttttatat ttggtgcttt agggtttact
     3061 atttatcaca ttgcattaaa ttacggcgaa aaaacagtaa atgctggatc tgcaagttta
     3121 attgtttcag tcacacctat agtaacggca atttttgctt ctattttttt gaacgaaaaa
     3181 atgaaattaa atggttggat cggtggtgta attagtttta taggaattgc tttcatatca
     3241 tttagtcaag gagatgctat tcaattgaat agtggaggat tatttatatt attagcagcg
     3301 attgcagaaa gcctattttt cgttttccaa acttcttact tgaaaaaata cggcttcttg
     3361 ccatttacta tatatacaat tttatctagt actgtatgta tgcttatttt cttaccggga
     3421 atgtatacag aaatactagc agctcctctt gaggtgaata tgagtgtttt atatttaggt
     3481 ttatttccaa cagtactccc atatatcgca ttagcttata tcatatctca ttctggtgct
     3541 actgaagcaa caagctctct atatttaacc ccagtaactg catgttttat tgcttggata
     3601 tggttaggtg aaataccgtc ttttgtttcg ataattggtg gaggaattac tatattaggg
     3661 gttttaattg cacatatatc attaaagaaa gaaatgcata gtgaggagta ctgcaataca
     3721 acaaagtagc ttttgtttag aggtaataat tcaatatatc tattaatgac atgtaaccac
     3781 gtaacttttg aaattgaaac agggaattat tttactgcgt tgaatatata aatacattct
     3841 cttaataaag gagctaaaat tattgaatgt ccagttaaag gttgttacga gagagaattg
     3901 ggaagatgca ttaaagttac aagttaaaga aaaccagatg aaatttgttc catctgtagc
     3961 agtttcactt gctaaagtat atataaaacc agatggtgat aatgtagagt acataccatt
     4021 tgcgatatac gacggcgatc taatagttgg ttttattatg catgctgttg taagagagac
     4081 aactaatatg tattggataa acggttttat tatcgataaa aagcagcaag gtaatggtta
     4141 tggaaaagcg gcattacaga aaaacatcta tttaataaaa aatacatgta aggcgtgtaa
     4201 agaaattaga ttaaccgtac ataaagataa tatctctgca aagaaactgt atgaacgtta
     4261 cggttttcaa tcattgggac atgaatatga tggtgagcaa gtctatcgtt tattcgttaa
     4321 tcaaatataa agtaaaggat tgagaggaaa agcaattgaa aactaaattt catcacattg
     4381 tacgagctgt tatgattaaa gataaaaaat tgttagtggc tgaatatatt ggccatcatt
     4441 attttttacc aggtggtcat gttgaagttg gggaatcagc tgagaatgca ttaataagag
     4501 aactgcaaga agaactcgga gttaactgta gtataaaaca atttttaggc gtcatagaaa
     4561 accaatggga agaccgagaa gttcttcatc atgaaattaa tcacatgttt gaaatagatt
     4621 cacaagagtt acatattgat ttcacaccaa actctaaaga gtctcattta gcatttcact
     4681 ggatagatta taatcaagaa gctttaaata cttataaaat catgccagca ccttcagtga
     4741 aagagttact agaaagaaaa ttaagcgatg aactactaaa ctgttggatt agtaattttt
     4801 aaatacctct attaaaaata aattcgtcta ctgaaaggag attaagaaaa taggctaaaa
     4861 aacaaggtac atcacctagt gtaagttcat attttgttga tgtacgcatt tagtctatag
     4921 cctttttcta ctttgtttgc aaaaatatat aaactgtaac atttggacgg aatttatttt
     4981 tataaatgga gggtttcgta atgaatcagt ttcaacaaga acttcaatca ttaaacctta
     5041 atgattatca atccggtaat gttgtgtatt gggatcaact gcaacaaaat caatatccat
     5101 actattacat tcaagatgat gcacgtcgtt gcggcggatg cggtggttgc ggcggacgct
     5161 gtggtggatg tggcggcgga agatgcggag gttgtgcagg acgttgcggc ggatgtatag
     5221 gttgcgctgg ttgtttcagt tgctttaatt gctggaactg gtggatcatt taagtataga
     5281 acatcaataa tatcttacat gaaagaaagg tgtaaatact attttatacg tttctttcat
     5341 gttgtgattt tttattcgtg ttaaggggga tttatttgta tacatacgat aaattgattt
     5401 cttgggtcga gaatataaaa gaagaaaatc atagttctgc aactgcactg tgtattataa
     5461 aagataataa aatcgactag agcattatag tggctatcat tcaaatacat ctacaaataa
     5521 caaagtaaca gcaccttcac aattcaatgt tgcttctgcg agaaaaagtt atttaggatt
     5581 aatggtagca tatgcgcttt acgagggaaa aataaactca attgatgatg aagcgaaaaa
     5641 atattttaaa gacttcgacc ctgtattact cggtaaaata acgataagac atttagtaac
     5701 tcattcgcac ggtttaggag aaacgaatga cgggacaaat taggaattac tggaccaacc
     5761 attttagtaa tacctgaata taaaggagtt gttgcgaaaa tgtacaataa aagatataac
     5821 tacggtggtg ataattactt atattattta cgtaaattta gcaatttagt cgttgataca
     5881 tttcataaca gtaatagggc ataattactc ctataatgaa aaggagtgaa agaaaatgaa
     5941 agataaaata aataaaaaca aaaagcaaaa tattaaagat gtaaatacag aacaaaatgc
     6001 aatttatagc gatccaaaag atgcagctaa tatgcaaacg gtaccgcagc cgaaggattt
     6061 tgatgaaatt gaatactaat tttttcttca tccgactata taatgaatga aaaatacatc
     6121 ttaagtccca tcacatgaaa gaaatctttt ggtatcatat atacataagc atttgaaaat
     6181 gtcttatctt aacaaaacta agctttcttt tagtaaatct aatataacct gcaaaaagac
     6241 acttcataat ggagtgtctt tttgtgtgta tgaacttaat ttccattttc cgcatagtat
     6301 cttgtatttt atgatggtta tactaactgt gaaaattcat gtcaagataa aagaagggga
     6361 cagttagtat gaaacaaact tctataaatc cattactaat tgttttaggt acaatcattg
     6421 ttcaaattgg ccttggaaca atttatacat ggagtttatt taatcaaccc cttgtaagta
     6481 agtttggatg gaacctcaat gccgttgcga taactttctc cataacaagc ttttctttat
     6541 cattttcaac cttattcgca ggtaagctgc agcaaaaatt aggacttcgt aaactcatcg
     6601 ctactgcagg gattgttttg ggactcggtt taatacttag ttcacaagtt tcttccttac
     6661 cattactata tttattagct ggtgtcgttg ttggttatgc agatggaact gcttatatta
     6721 catcactatc taatttaatt aaatggtttc caaatcggaa aggacttatt tctggtatat
     6781 ctgtttctgc atatggaatg ggcagcttaa tctttagata tataaacggt agtcttatcg
     6841 atagtcttgg ggtatcacaa gcatttttat attgggggat tatcgtccta cttttagtat
     6901 taaccggatc gttcttctta cgtgaagcag ttgtaagtaa caccgtaact gagacattac
     6961 acagtgacta tacgtctcgt gaaatgatgc gaacgaaaca agtatatctc ttgtttttca
     7021 tgttattcac atcatgtatg ggtggtctat atttaatcgg tatggtaaaa gatatcggag
     7081 tacagctcgt tggacttagc gcagcaaccg ccgctaacgc cgtcgcaatg attgcaatct
     7141 ttaatacagt aggtagaatc attcttggaa cattatcaga taaaattggc cgaatgaaaa
     7201 ttgtctctgc aacgtttatt attattggat tgtcagtctt tactttaagt ttcatcccac
     7261 taaattacgg tatttatttc gcttgtgtag caagtgttgc cttttgcttt ggtggtaaca
     7321 ttacaatatt cccggccatt gtcggagatt tcttcggctt aaaaaatcat agtacaaatt
     7381 acggaattgt ttaccaaggt ttcggatttg gtgcgcttgc aggatcattc atcggagcac
     7441 tacttggtgg atttcaaccg acctttatta taattggttt tctatctgtt atctctttcg
     7501 ttatttcaat tttaattcgt cctccaaaag cagagaagaa aaaggaaata aaacatttac
     7561 atcggaaagt agcctaatac tgaaaagggt cctaatatag ggctcttttt tatatagtaa
     7621 tgaaaggtta ttgattatta attttgctaa gagaagataa atgaaatact ctattaactt
     7681 ttttatattt taaaacgatt aaatggtaga atgtaacaaa gacagaaaat tcctgttatt
     7741 agaggtggac ttagatgaaa aaagtaaatg ttacatatgc acttttatac gataaaacaa
     7801 atgaaaaggt cttaatggta aaaaacaaag ggaagaaagg ttcttattac acgctaccag
     7861 gtggcgcagt taaatttggg gaaaccttag aagaggcagt aatccgggaa gtgaaagaag
     7921 aaaccgggct acatataaat gtaaacggta tctgttacat tagcgaagct tttttcgaag
     7981 agagaggaca tcatgcaatt ttctttaatt ttttaggtga aataattggc ggggagacaa
     8041 atataacaag gccaaaagaa attgaagaga ttacttggat ggaattacat agagcagcac
     8101 cgcatttacg tataccagaa cacttattaa atacattaca aagaaaagaa acagtacctt
     8161 acttttttaa cggaacaatc attcatcaat cttcataaca ttatcatttc atgtaaggcg
     8221 gggaaaaaat ggagtttatc gttaatcata aacatttcac tcaggcactt tcagaagtaa
     8281 gtaaagctat atctactaaa gctataatcc ctatactatc cggcataaaa ataattgcag
     8341 atcaatctgg aattacttta atcgcaagta attcaaatat tttcatcgaa aaattcatcc
     8401 ctaattccaa cgaagacgac caaattacaa ctatagtaaa agcaggaact attgtcgtac
     8461 ctgcaaaata tttcattgaa attataaaga aaatgcctag cgatgtatca ataaaaagta
     8521 cgaatgagca aatcattacg atacaatcag aagaaattac attaaactta aatggacttc
     8581 cagcatatga gtttccgaat gtaccactta tagatgatca tgcaaaagta caagttaaaa
     8641 cagaacaatt gattgaagtg tttaaacaaa ctgcctttgc ggtagctaaa aatgaatcta
     8701 gacccgttct aaccggagta catatcgagt tagaccacaa caaattaatt tgtgctgcaa
     8761 ctgattcaca tagacttgct atacgtgaaa tgataatttc ttcagatgtg aaagcaaatt
     8821 gtatcgtacc aagtgcaact attagtgaac tgctaaaatt aatgagcagt aattcaaaat
     8881 ttgtatacat ctacctttca gaaagtcaca ttatttttac attcggtacc actacgttat
     8941 attcaaggct aattgaaggg aaatatccta atatatctag tttaattcca aatgattttc
     9001 aaacagtcat taacatagat agaaaaagga ttttacaagg tgtagatcga tcaagcttat
     9061 tagcaagtga atgggcaaat aacaatgtca acttagaaat cataaacgaa tccacaatta
     9121 aaatttcttc taacgcttct cagattggaa aaatatccga gacacaacag atagatacga
     9181 ttcaaggtga aaaacaatta aatatatctt tcgatggacg atttatgttg gatgctttaa
     9241 gggcgataaa agaagaaacg gttactttaa gtttcagtgg ttctatgaga cccatattaa
     9301 ttgaagcagg gacgcaatct gcagcagtac atcttatatc accagtaaga gcttattaaa
     9361 aacataggaa ggaatcacat tatgtacaag catactttat gttttataaa gagaaatgaa
     9421 gaaatactta tgttaaatag agaatatgac cctgtaaaag gattatggaa cggggtaggg
     9481 ggaaagatag aaaagggaga aacaccttta gacaatgcga ttcgtgaaat aaaagaagag
     9541 actaatatag aagttaagca tgaccaaatt cggtttaaag gtattattaa atgggaggac
     9601 tcttcatatt ctggcggtat gtatgtttat ctagtagagt tacttaatga atttacatat
     9661 cacactccaa aaaaagtatc agaaggaatt ttagattgga aagagatttc ttggattcta
     9721 agtgactata attacggcgt aggagagatg ataccaaaat ttttatatgc actactccac
     9781 aatgaattaa tattagagca caactttgtt ttatctaatc ataaattaat agattattgg
     9841 aatgaagaat tagctaagca ggacagttct atagataata tagttcaatt gtaataagaa
     9901 taaatttatc ccaaagtaga gtttcaaata gcaagaaggc aaacatagtg aaatacattt
     9961 aaatacagaa aaaagagcga atcacttata gaaaggtgcg ctcttttttt tatttataat
    10021 taaatttctc tccaccaggc tgatgaagga tagtctgtag atttcaacgt aatagattca
    10081 ccgatttgtg gcgttgcaat attaacccct aaacgattcg cttcttttgt cacacgttca
    10141 ataggatcac tccattcatg taacgccaat gtaaaagcac cccaatgaat cggaagaagc
    10201 agttctcctt tcacgtcaat atgagcttgt accgtctctt caggtaacat atgaatagca
    10261 gaccatctag gat
//
