LOCUS       NZ_CP044407            10909 bp    DNA     circular CON 28-JUL-2024
DEFINITION  Actinomadura sp. WMMB 499 strain WMMB499 chromosome, complete
            genome.
ACCESSION   NZ_CP044407
VERSION     NZ_CP044407.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura sp. WMMB 499
  ORGANISM  Actinomadura sp. WMMB 499
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 9187113)
  AUTHORS   Braun,D., Bugni,T.S., Chevrette,M.G. and Currie,C.R.
  TITLE     Draft genome of Actinomadura sp. WMMB499
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9187113)
  AUTHORS   Braun,D., Bugni,T.S., Chevrette,M.G. and Currie,C.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2019) Pharmacy, University of Wisconsin-Madison,
            777 Highland Ave., Madison, WI 53705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. Oct-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008824145.1-RS_2024_07_28
            Annotation Date                   :: 07/28/2024 05:00:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,523
            CDSs (total)                      :: 8,413
            Genes (coding)                    :: 8,251
            CDSs (with protein)               :: 8,251
            Genes (RNA)                       :: 110
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 64 of 162
            Pseudo Genes (incomplete)         :: 108 of 162
            Pseudo Genes (internal stop)      :: 10 of 162
            Pseudo Genes (multiple problems)  :: 19 of 162
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:12:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8001792
            Orig. end                         :: 8012701
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044407.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Genome not circularized.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10909
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10909
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10909
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5909](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5909
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    804..824
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 18.87"
                     /tool="antismash"
     gene            1248..2081
                     /locus_tag="F7P10_RS36430"
                     /old_locus_tag="F7P10_36430"
     CDS             1248..2081
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581704.1"
                     /locus_tag="F7P10_RS36430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36430"
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="WP_151016603.1"
                     /transl_table=11
                     /translation="MLWFWILLRIVLTPIMYLLWRPRNRGMRNVPKRGPALMVSNHLSF
                     ADHFFGPLPLMRPILFIGKGEYFTGKGIKGLISKGFFTGVGVVPVDRTGGSAAEAALQT
                     GLRILGEGKLLGIYPEGTRAPDNRLYRGKTGVARLALKSRVPVIPMAMINTFELMPPGK
                     PLPRLGVRPGVRFGEPLDFSRYYGREDDREVLRAITDEIMAAIRELSGQEYVDRYAAEV
                     KAEMAGKPVRPVRDDDDDDENDDDENDEDENDAGENDGGAAARTGRDDGGRTDGG"
     gene            2137..3291
                     /locus_tag="F7P10_RS36435"
                     /old_locus_tag="F7P10_36435"
     CDS             2137..3291
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630856.1"
                     /locus_tag="F7P10_RS36435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36435"
                     /product="MacS family sensor histidine kinase"
                     /protein_id="WP_254716208.1"
                     /transl_table=11
                     /translation="MYRTAALGYAAVLIVKNSGGYAHPAGAWAVLGVMSGWTVAAWLLF
                     RDPRRRGPALLAADMAISAGCVLATAWVESVAGIVSGRPTLPVSWVAAAVLSWAVVGGR
                     RLGIAAAGVLALANLLVHVFAGTTGEIDTRALNGIVLLVLAGLVVGHVVRLAREAEARL
                     ARAVELESAGRERERLARDIHDSVLQVLAMVRRRGAGLDGEAAELARLAGEQETALRAL
                     VGSAPRTPPRAAAGSGRPAAGAAPGRPGPAAGDDRDLRPLLTARASGTVTVSTPATPVP
                     LPEHAARELDAAVAAALDNVAVHCGDAARAWVLLEDGGDEVIVSVRDDGPGMPAGRLEQ
                     AAADGRLGVAQSIRGRVRDLGGTTLITSAPGEGTEIEMTVPRGK"
     gene            3312..3986
                     /locus_tag="F7P10_RS36440"
                     /old_locus_tag="F7P10_36440"
     CDS             3312..3986
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909401.1"
                     /locus_tag="F7P10_RS36440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36440"
                     /product="response regulator transcription factor"
                     /protein_id="WP_151016604.1"
                     /transl_table=11
                     /translation="MSEAPLRVMVVDDHPMWRDAVARDLAEAGHEVVATAGEGRAAVRA
                     APAARPQVAVVDLQLPDISGVEVTRHLAAADPPVRVLVLSASGEQEDVLEAVKAGATGY
                     LLKSAAREEFLDAVRRTAAGDAVFTPGLAGLVLGEFRRLAAAPARDAGDGAPRLTERET
                     EVLRLVAKGLTYKQIAQRLVLSHRTVQNHVQNTLGKLQLHNRVELVRYAIEQGLDDDLG
                     KG"
     gene            4114..4983
                     /locus_tag="F7P10_RS36445"
                     /old_locus_tag="F7P10_36445"
     CDS             4114..4983
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="F7P10_RS36445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36445"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_151016605.1"
                     /transl_table=11
                     /translation="MADPPEHAPAFGLFSLEVAWTVIVPVSVLILAGALALRRRYSRVP
                     PLGREPRRYEAALLSGGESRAVLTGVAVLRTERAIEARGPRRVGVTEGGRGRGEPLDDA
                     LYEAIGPEWDLSTLKDRTVVEDALRRLRDGMERDGLLLSPDQRADRRAAALIPVIGTVV
                     GSFVIIAGGLRAGVTAPWIAAALVEAALAVTALILLADPVSRTGEGVRAWRACRERHSH
                     LDPANSPSWTTYGAAGVAMGVALFGAQALAGADPEFAAETEIVDVFEGNGGTNGGGCGG
                     CGGCGGCG"
     gene            5001..5909
                     /locus_tag="F7P10_RS36450"
                     /old_locus_tag="F7P10_36450"
     CDS             5001..5909
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="F7P10_RS36450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36450"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_218040238.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-73, bitscore: 245.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRAARGGDGAPRTAVAAGAGGPSGRAGTDAVPAGLGVGIGWRPP
                     ITGFVSALPGLRFTEVIAESVRPDRPASDLLALRDRGAAVVPHGVKLSLGGAEPVADER
                     VAHLAACAEALGAPLVGEHVAFVRAGGIEAGHLLPVPRTRAALNVLTANVRRTLDGLPV
                     PLAVEPIAALFDWPDAEYDEAGFLTELVERTGVLLLLDVANVYANARNRGADPLHLLDR
                     LPLERVAYCHVAGGDQAGGRYHDTHAAPVPREVLDLLAELCARHRPPGVLLERDGRYPP
                     AAELRDELDAIADASGHPRIT"
     gene            5906..6355
                     /locus_tag="F7P10_RS36455"
                     /old_locus_tag="F7P10_36455"
     CDS             5906..6355
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F7P10_RS36455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="F7P10_36455"
                     /product="hypothetical protein"
                     /protein_id="WP_151016607.1"
                     /transl_table=11
                     /translation="MTGEPLDADLAAAQEALVRAMTAGGPVPPGFDAGAVRAAARGILR
                     KRAGEVAHAWPALAASYGTSWGEAFAAWAADRPTRGSARDGRDFARARRDELAPGAARE
                     LALAEARWSYGREGAPRRRAAGIRRMPGGLAVAVLGRAWTAARRR"
     misc_feature    complement(6396..6411)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 25.87"
                     /tool="antismash"
     gene            6520..7545
                     /locus_tag="F7P10_RS36460"
                     /old_locus_tag="F7P10_36460"
     CDS             6520..7545
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /locus_tag="F7P10_RS36460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36460"
                     /product="6-phosphofructokinase"
                     /protein_id="WP_151016608.1"
                     /transl_table=11
                     /translation="MRVGVLTGGGDCPGLNAVIRAVVRKGVQVFGYEFVGFRAGWRGPL
                     EGDTVALDVQAVRGILPRGGTILGSSRTNPIKVEGGVERIKDNLAGLGVDALIAIGGED
                     TLGVARELHSRGVHVVGVPKTIDNDLNATDYTFGFDTAVNIGVEAVDRLHTTAESHHRA
                     LICEVMGRHAGWIALHVGMAAGANVILIPERPFDIDKVVEYVESRFKTRYAPIIVVSEG
                     AHPVDGQMQLQTGERDAFGHVRLGGIGQRLADEIEKRTGKEARATVLGHIQRGGTPSAF
                     DRVLATRLGLQAIDAVRDGDYGKMVALQGTDIVRVGLDEATKELKTVPVERYEEAEVFF
                     G"
     gene            7651..8886
                     /gene="thiI"
                     /locus_tag="F7P10_RS36465"
                     /old_locus_tag="F7P10_36465"
     CDS             7651..8886
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630853.1"
                     /locus_tag="F7P10_RS36465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36465"
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="WP_151016609.1"
                     /transl_table=11
                     /translation="MTTLDAAPAVVREQSPVDGEPCVLLKLGEVVLKGKNREQFERRLA
                     DNVRTAVRPIARVDVIRRHGVFIVRMHDADVPTVDRIAQRIGDVMGIVWAHRAWRVGKD
                     LASVERAALELMDGRAGSFAVRSRRRDKRFPMTSTELDRHIGALVAAKYGQPVRLKNPE
                     HTLSIEVDRDEVFVFSGGLPGQGGLPVGMSGRALVLMSGGIDSPVAAYRMMRRGLRVDY
                     LHFSGMPFTGPESIYKAYALVRELDKFQGGSRLFVVPFGKAQQQIKSSGADRLAVVAQR
                     RLMLRTGQLLARRLRGAALITGDALGQVSSQTLANITALDDAVELPILRPLVGMDKTEI
                     MDQARRIRTLSISELPDEDCCTMLAPRRAETRAKIDDLRQIEKRLDVGELADRLVESVQ
                     EHRPVYGDAAAS"
     gene            complement(8934..9818)
                     /locus_tag="F7P10_RS36470"
                     /old_locus_tag="F7P10_36470"
     CDS             complement(8934..9818)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039603.3"
                     /locus_tag="F7P10_RS36470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36470"
                     /product="DUF5954 family protein"
                     /protein_id="WP_151016610.1"
                     /transl_table=11
                     /translation="MTTGEGDAGGRLFPEDLDGVDPVAAVLRADARRAMTAYPEPVAVG
                     ALFAAAERVGGGWRLVCPCDPLPQGARELLAVHLEDRAAAADGTTGRELRAAARTLQAD
                     PSDEVSTAGLRFRIVRIEQLVRTGPDGPEPPRPTDLDPSGRAPRGEPDLLPGDESGADL
                     TSAELLCQVLDAAAATGNEPDGTFLTPVPLAPVFTVAERGGGRWRPVGRLHDGPQQARD
                     SLVTYFRHVVPVIELPGEPAAAEFAAAAELMEDGTGRNGITVAGRRFRVVRIERITLLG
                     PDGPEPPRPGDPR"
     misc_feature    complement(9854..9869)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.28"
                     /tool="antismash"
     gene            complement(9872..10870)
                     /locus_tag="F7P10_RS36475"
                     /old_locus_tag="F7P10_36475"
     CDS             complement(9872..10870)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572614.1"
                     /locus_tag="F7P10_RS36475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F7P10_36475"
                     /product="EamA family transporter"
                     /protein_id="WP_151016611.1"
                     /transl_table=11
                     /translation="MSLRTPSFGRSARTSPSTSPSTSPSGHAPASGPSGRGLPGGGTLP
                     ILAAASLWGTTGTARTFADGASSLSVAAMRIVLGGLVLLASAALLRDGTVRGAGLRRLA
                     ADRGARPLVLLGALAIAVYQTAFFVAAARTGVAIATVVTIGSAPAFAGLIGVAVRRTVP
                     GLRWTFATAAAVTGCALLAGGGADAGADPAGIGLAALSGLAYAAYTTVTAALLARGGEE
                     RAVVGTLFGVAAVPLLPLLPFAGPAWMLTGSGALIALYLGVVATGGAYLLFARGLRTTP
                     ATAATTLTLAEPAVAAVLGVALLGEHLGGPALAGLALIAAALPLIALPARK"
ORIGIN
        1 gcggcgacgg cgaggaacgc ggcccagccg ggcacctccc agtcgaggat gacggtgatc
       61 agcaggtaga ccggcccgcc gaccagcgcg acccaggcgc ccttggtgag cgggtccgcg
      121 gcgggcagcg gcgggggagg cggcgggacg tagtggccct cctcgtcctc cgccgggaac
      181 tccggctccg cggccggctt gatcacccgg gcgcggggca gccggccggt gcggtcgtcg
      241 ccctccgcgg gcggttcggg cggctcgtcg atgttctcct ggtcgggcca gggcacctcg
      301 tcgccctcgg gtacggcgtc gaaccccgcg acgatctcgg cccagatggc gtcctcgtcg
      361 cgttcggcgg tgccggcggg ggacgcgtcg gccgcgccgg gcccgtccgg gccgcccgcg
      421 ccggcgggct cgggacggtc ggcgcgcggc cggtcgccgt ccgcgcggtc ggggtcggag
      481 cgcttgccgt ccgcgcgctc gccgtcgggc cgtcccgggt cggtgagcgc ggcgtcggtg
      541 cggtcggagt ccgggtcgga accgcggacg cccgcgtcgc gcagccgcgc caggtgggcc
      601 cgcaggatcc cctcggcggc cggcttcatc tcgacgtcca cgtacagccg gtcgaggacg
      661 tcctcggcgg cgcgtccgga cccgtccgcg gcgcgcggcg ccccgtcggg cggaccggac
      721 ggggtgagcc ccgggtcgtc cgcgccggtc ccggcggccg gcgggacggc gtaggccgcc
      781 acgcccgcgt cccggagcgc cgtcagcatc gcgtcggcga gatgcggggc gagatcgacc
      841 agcggggcgt aggtgtcggc ggacagtcca ttgccacggc gattcacggg gccggctcct
      901 tcgactccgg cgtgaccggc agcaccggca tgtcggcctc cgctccccca tcgacaggcg
      961 cgtccggcgg cgctccgcac acggcggcgc ccccggacac gtagaaacgt gtgtcccacg
     1021 gtattcgctg caccgcccgg gacaagcccc ggggcgcacc cgaattgaga ttacgtagag
     1081 agactacggc catccggcgc cgcgccgcgc cccgtccggc gggcccggcg ggacggccgg
     1141 gtgggcggcc gcggggcgag atgcgaagat agcccatcat cttccccccg ggccggggac
     1201 ctcgtctcgc acaccgcatg cacgcgcggt taggaaaggg tgtcggcatg ctctggttct
     1261 ggatcctgct gaggatcgtc ctcacgccca tcatgtacct cctgtggcgg ccgcggaacc
     1321 gggggatgcg caacgtgccg aagcgcgggc cggcgctcat ggtcagcaac cacctgtcgt
     1381 tcgccgatca tttcttcggg ccgctgccgc tgatgcgtcc gatcctgttc atcggcaagg
     1441 gcgagtactt caccggcaag gggatcaagg gcctgatcag caaggggttc ttcaccggcg
     1501 tgggggtggt gccggtcgac cggaccggcg gctcggccgc cgaggccgcc ctgcagacgg
     1561 gcctgcggat cctgggggag ggcaagctgc tcgggatcta cccggagggc acccgcgccc
     1621 ccgacaaccg tctgtaccgg ggcaagacgg gcgtggcgcg gctcgcgctg aagtcgcgcg
     1681 tcccggtgat cccgatggcg atgatcaaca cgttcgagct gatgccgccc ggcaagccgc
     1741 tgccccggct cggcgtccgg ccgggcgtcc ggttcggcga gccgctcgac ttctcccgct
     1801 actacggccg cgaggacgac cgggaggtgc tgcgcgcgat caccgacgag atcatggcgg
     1861 cgatccggga gctgtccggc caggagtacg tcgaccggta cgccgccgag gtcaaggccg
     1921 agatggcggg caagccggtc cgcccggtgc gcgacgacga tgacgacgac gagaacgacg
     1981 acgacgagaa cgacgaggac gagaacgacg ccggcgagaa cgacggcggg gccgcagccc
     2041 gtaccgggcg cgacgacgga gggcggaccg acggcgggtg aaccggaccg cggcggcggc
     2101 ctggggctgg aggccgcgct gtggcgggcg gtcgcggtgt accggaccgc cgcgctcggt
     2161 tacgccgcgg tgctgatcgt caagaactcg ggcggctacg cgcatccggc cggcgcctgg
     2221 gccgtcctcg gggtcatgtc cggctggacg gtcgccgcgt ggctgctgtt ccgcgacccg
     2281 cgccgccgcg gccccgccct cctcgccgcc gacatggcga tctcggccgg ctgcgtgctc
     2341 gcgacggcgt gggtggagag cgtcgcgggg atcgtctccg gacggccgac gctgccggtg
     2401 tcgtgggtcg cggcggccgt cctgtcgtgg gccgtggtgg ggggccgccg cctcggcatc
     2461 gccgccgccg gggtgctcgc gctggcgaac ctgctggtcc acgtgttcgc cgggacgacc
     2521 ggcgagatcg acacccgcgc cctcaacggg atcgtgctgc tggtcctcgc cggactggtc
     2581 gtcgggcacg tcgtccggct ggcgcgcgag gccgaggcgc ggctcgcccg cgccgtcgaa
     2641 ctggagtcgg ccggacgcga gcgcgagcgg ctcgcccgcg acatccacga ctcggtgctg
     2701 caggtcctgg cgatggtgcg gcggcgcggc gccgggctcg acggcgaggc ggccgagctg
     2761 gcccggctgg cgggggagca ggagaccgcg ctgcgcgccc tggtcggcag cgctccccgc
     2821 accccgccgc gcgccgcggc cggttccgga cgtcccgccg cgggggccgc cccgggccgt
     2881 ccgggacccg ccgccgggga cgaccgggac ctgcggccgc tgctgacggc ccgagcgtcc
     2941 ggcaccgtca ccgtctccac gcccgcgacg cccgtcccgc tgcccgagca cgccgcccgg
     3001 gaactggacg cggccgtcgc cgcggcgctc gacaacgtcg ccgtgcactg cggtgacgcc
     3061 gcgcgggcct gggtgctgct cgaggacggc ggcgacgagg tgatcgtcag cgtccgcgac
     3121 gacgggccgg gcatgccggc cgggcgcctc gagcaggcgg cggcggacgg acggctcggc
     3181 gtcgcccagt cgatccgcgg gcgcgtccgc gacctcggcg gcacgaccct gatcacgtcc
     3241 gcgcccggtg agggcaccga gatcgagatg accgttccgc gtggaaagtg aaggcgtgag
     3301 aaggtgaggc cgtgagcgag gcacccctca gggtcatggt ggtggacgac cacccgatgt
     3361 ggcgggacgc ggtcgcccgc gacctggccg aggccgggca cgaggtggtc gcgaccgcgg
     3421 gcgagggccg cgccgcggtc cgcgccgccc ccgccgcgcg tccccaggtc gccgtcgtcg
     3481 acctgcagct ccccgacatc tccggggtcg aggtgacgcg gcacctcgcc gccgccgacc
     3541 cgcccgtccg ggtgctggtg ctgtcggcca gcggcgagca ggaggacgtg ctggaggccg
     3601 tcaaggcggg cgccaccggc tacctgctga agtcggcggc ccgggaggag ttcctggacg
     3661 ccgtccggcg caccgccgcc ggcgacgccg tcttcacccc gggcctcgcc gggctcgtcc
     3721 tcggcgagtt ccgccgcctc gccgccgccc ccgcccgcga cgcgggcgac ggcgcgcccc
     3781 ggctcaccga acgcgagacc gaggtgctgc gcctggtcgc gaagggcctc acctacaagc
     3841 agatcgcgca gcgcctcgtc ctgtcccacc gcaccgtgca gaaccacgtg cagaacaccc
     3901 tcggcaagct ccagctgcac aaccgcgtcg aactcgtccg ctacgcgatc gagcagggcc
     3961 tcgacgacga cctcggcaag ggctgaccgg aacggccgcc cgcggccgga tccggccgcg
     4021 ggcggcggga caagacaggg aacaccccgc ctcttccggg cgtcgaagcc aacaactcga
     4081 catatcggac atcggcgcgc cggggaggcg gctatggcgg atcctcccga gcacgccccc
     4141 gcgttcggcc tcttctccct tgaggtcgcc tggacggtca tcgtccccgt gtccgtgctg
     4201 atcctggccg gtgccctggc gctccggcgc cggtactcgc gggtgccccc gctggggcgc
     4261 gagccgcgcc gctacgaggc cgctctgctg agcggcgggg agagccgcgc ggtcctcacc
     4321 ggcgtggcgg tgctgcgcac ggagcgggcc atcgaggcgc gcgggccccg cagggtcggc
     4381 gtcaccgagg gcgggcgggg gcggggcgag ccgctggacg acgccctcta cgaggccatc
     4441 gggccggagt gggatctgtc gaccctgaag gaccggacgg tggtcgagga cgccctgcgc
     4501 cggctgcggg acggcatgga acgcgacggt ctgctgctgt cgccggacca gcgcgccgac
     4561 cgccgggccg ccgcgctcat cccggtcatc gggacggtcg tgggctcgtt cgtcatcatc
     4621 gccggcggcc tgcgcgcggg cgtgacggca ccctggatcg ccgcggcact ggtcgaggcg
     4681 gcactcgccg tcacggctct gatcctgctg gccgaccccg tgtcccggac cggcgaaggg
     4741 gttcgtgcct ggcgggcctg cagggagcgg cacagccatc tggaccccgc gaactcgccg
     4801 tcctggacga cctacggtgc cgcgggggtg gccatggggg tggcgctgtt cggcgcgcag
     4861 gccctggccg gcgcggatcc ggagttcgcg gcggagacgg agatcgtgga cgtcttcgaa
     4921 gggaacggcg gcaccaacgg cggcggatgc ggaggctgcg gcggatgcgg aggctgcggg
     4981 tgatcccgcg gccgaggcga gtgaggcgcg ccgcccgcgg cggggacggc gctccgcgga
     5041 ccgccgtggc cgccggggcg ggcggaccgt ccggccgcgc gggaacggac gcggtccccg
     5101 ccgggctcgg ggtgggcatc gggtggcggc cgccgatcac cgggttcgtc tcggcgctgc
     5161 cggggctgcg gttcaccgag gtgatcgccg aatcggtgcg cccggaccgt cccgcctccg
     5221 acctgctcgc cctgcgcgac cgcggcgcgg cggtcgtccc gcacggggtg aagttgtcgc
     5281 tcggcggcgc cgagccggtc gcggacgaac gggtcgcgca cctggccgcg tgcgccgagg
     5341 cgctcggggc gccgctggtc ggcgagcacg tcgcgttcgt ccgcgcgggc gggatcgagg
     5401 ccgggcacct gctgccggtg ccgcgcaccc gggccgcgct gaacgtcctc accgccaacg
     5461 tccgccgcac gctcgacggg ctgccggtgc cgctggccgt cgagcccatc gccgcgctgt
     5521 tcgactggcc ggacgccgag tacgacgagg ccggcttcct caccgaactg gtcgagcgca
     5581 cgggcgtcct gctgctgctc gacgtcgcca acgtgtacgc caacgcccgc aaccggggcg
     5641 cggacccgct gcacctcctc gaccgcctgc ccctcgaacg cgtcgcgtac tgccacgtcg
     5701 cgggcggcga ccaggcgggc gggcggtacc acgacaccca cgccgcgccc gtcccgcgcg
     5761 aggtcctcga cctgctcgcg gagctgtgcg cccggcaccg cccgcccggg gtcctcctcg
     5821 aacgcgacgg ccgctacccg cccgccgccg aactgcggga cgaactcgac gcgatcgccg
     5881 acgcgtccgg ccacccccgc atcacatgac cggggagccc ctggacgcgg acctggcggc
     5941 ggcgcaggag gcgctggtgc gggcgatgac cgcgggcggc ccggtaccgc cggggttcga
     6001 cgcgggcgcc gtccgggccg ccgcgcgcgg catcctgcgc aagcgggcgg gggaggtcgc
     6061 gcacgcgtgg cccgcgctgg ccgcgtcgta cgggacgtcc tggggcgagg cgttcgcggc
     6121 ctgggcggcg gaccggccga cgcgcggatc cgcccgggac gggcgcgact tcgcccgcgc
     6181 ccgccgcgac gagttggcgc ccggcgcggc gcgggaactg gccctcgcgg aggcgcggtg
     6241 gtcgtatggc cgcgagggcg cgccgcggcg ccgggccgcc gggatccggc ggatgcccgg
     6301 cgggctcgcg gtggcggtcc tcggccgggc gtggacggcg gcccgccgcc gctgacgggc
     6361 ttcgcggcgg acgtgccgaa gcccgcccga acgactatgg tctagaccaa tggacgggcg
     6421 gccggaacgg gcggcgaaca cgccccgtgg ccgcgcgccc acggatagtc tgagcacgtg
     6481 aatcgaggga ggagaccggt ggcgggacaa ggagacggca tgcgggtcgg agtgctgacc
     6541 ggcggcgggg actgccccgg cctgaacgcg gtgatccgcg cggtagtgcg caagggcgtg
     6601 caggtcttcg ggtacgagtt cgtgggcttc cgggccgggt ggcgcgggcc cctcgaaggc
     6661 gacaccgtgg ccctcgacgt ccaggccgtg cggggcatcc tgccgcgcgg cggcacgatc
     6721 ctcgggtcgt cccggaccaa cccgatcaag gtcgagggcg gcgtcgagcg gatcaaggac
     6781 aacctggccg ggctcggcgt ggacgccctg atcgccatcg gcggcgagga caccctcggc
     6841 gtcgcgcgcg agctgcactc gcgcggcgtg cacgtcgtcg gcgtccccaa gacgatcgac
     6901 aacgacctga acgccaccga ctacaccttc gggttcgaca ccgcggtgaa catcggggtg
     6961 gaggccgtcg accggctgca caccaccgcc gagagccacc accgcgcgct gatctgcgag
     7021 gtcatgggcc ggcacgcggg ctggatcgcg ctgcacgtcg gcatggccgc cggcgccaac
     7081 gtcatcctga tcccggagcg gccgttcgac atcgacaagg tcgtcgagta cgtggagagc
     7141 cggttcaaga cccggtacgc cccgatcatc gtggtctcgg agggcgccca cccggtcgac
     7201 gggcagatgc agctgcagac gggcgagcgg gacgcgttcg ggcacgtccg gctcggcggc
     7261 atcgggcagc ggctcgccga cgagatcgag aagcgcaccg gcaaggaggc ccgcgccacc
     7321 gtgctcgggc acatccagcg cggcggcacc ccgagcgcgt tcgaccgggt cctcgccacc
     7381 cgcctcggcc tgcaggcgat cgacgccgtc cgcgacggcg actacggcaa gatggtcgcg
     7441 ctgcagggca ccgacatcgt ccgggtcggc ctcgacgagg cgaccaagga gctgaagacc
     7501 gtcccggtgg agcgctacga ggaagccgag gtcttcttcg gctgaccggc gtcctccccg
     7561 gcgggaacac gcatacccgg tcgcggcgtt gcggcttctg ggccacacta gaggccccac
     7621 ccccgatcga cgcccgccag gaggatccgc atgaccacgc tcgacgccgc gcccgccgtc
     7681 gtccgggagc agtcgccggt cgacggcgag ccgtgcgtgc tgctcaaact gggcgaggtc
     7741 gtcctcaagg gcaagaaccg cgagcagttc gagcgccggc tggccgacaa cgtccgcacc
     7801 gccgtccgcc cgatcgcccg cgtcgacgtc atccggcggc acggggtgtt catcgtgcgc
     7861 atgcacgacg cggacgtccc cacggtcgac cggatcgcgc agcggatcgg cgacgtcatg
     7921 ggcatcgtgt gggcgcaccg cgcgtggcgg gtcggcaagg acctcgccag cgtcgagcgc
     7981 gccgccctcg agctgatgga cgggcgggcc ggctcgttcg ccgtccgctc gcggcgccgc
     8041 gacaagcggt tcccgatgac ctccaccgag ctcgaccggc acatcggcgc gctcgtcgcc
     8101 gcgaagtacg ggcagccggt ccggctcaag aaccccgagc acaccctgtc gatcgaggtc
     8161 gaccgcgacg aggtgttcgt gttctccggc gggctccccg gccagggcgg cctcccggtc
     8221 gggatgagcg ggcgcgcgct cgtgctgatg tcgggcggca tcgactcgcc cgtcgccgcc
     8281 taccggatga tgcgccgcgg cctgcgcgtc gactacctgc acttctccgg gatgccgttc
     8341 accggccccg agtcgatcta caaggcgtac gcgctggtcc gcgagctgga caagttccag
     8401 ggcgggtcgc ggctgttcgt cgtcccgttc gggaaggcgc agcagcagat caagtcctcc
     8461 ggcgcggacc ggctcgcggt ggtcgcgcag cgccggctga tgctgcgcac cggccagctc
     8521 ctcgcccgcc ggctgcgcgg cgccgcgctg atcaccgggg acgcgctcgg ccaggtgtcc
     8581 agccagacgc tcgccaacat caccgcgctg gacgacgcgg tcgagctgcc gatcctgcgc
     8641 cccctcgtcg gcatggacaa gaccgagatc atggaccagg cgcggcggat ccgcaccctg
     8701 tcgatctccg agctgccgga cgaggactgc tgcacgatgc tcgcgccgcg ccgcgccgag
     8761 acccgcgcga agatcgacga cctgcgccag atcgagaagc gcctcgacgt cggtgagctc
     8821 gccgaccggc tcgtcgagtc cgtccaggag caccgcccgg tctacggcga cgccgccgcc
     8881 tcctgacgcc cggcccggcg ccgaccgcgg gacgtccggg gacgggacgg gcgtcagcgc
     8941 gggtcgcccg ggcggggcgg ctcgggcccg tccgggccca gcagcgtgat ccgttcgatc
     9001 cgcaccaccc ggaaccggcg cccggcgacc gtgatgccgt tgcggcccgt gccgtcctcc
     9061 atgagctcgg ccgccgccgc gaactcggcc gccgcgggct ccccgggcag ctcgatcacc
     9121 gggacgacgt gccggaagta ggtgacgagc gagtcccggg cctgctgcgg gccgtcgtgc
     9181 agcctgccga ccgggcgcca ccgcccgccg ccccgctcgg cgaccgtgaa gaccggcgcg
     9241 agcggcaccg gcgtcaggaa cgtcccgtcc ggctcgttcc cggtggcggc cgccgcgtcc
     9301 agcacctggc acagcagctc cgccgaggtg aggtcggcgc cggactcgtc cccgggcagc
     9361 aggtcgggct cgccgcgcgg cgcccgcccg gacgggtcca ggtcggtggg gcgcggcggt
     9421 tcgggcccgt ccggtccggt gcggaccagc tgctcgatcc gcacgatccg gaaccgcagc
     9481 cccgccgtgc tcacctcgtc ggacgggtcg gcctgcagcg tccgcgccgc cgcccgcagc
     9541 tcccgtccgg tggtgccgtc cgccgcggcg gcccggtcct ccaggtggac ggccagcagc
     9601 tcgcgcgcgc cctgcggcag cgggtcgcac gggcacacca gccgccaccc gcccccgacc
     9661 cgctcggccg ccgcgaacag cgcgccgacg gccaccggct ccgggtacgc cgtcatggcg
     9721 cggcgcgcgt ccgcgcgcag caccgcggcc acggggtcga cgccgtccag gtcctccggg
     9781 aacagccgtc ccccggcgtc cccctcgccc gtcgtcatgc cggccatcat ccctcccgga
     9841 cccggcctcc gcgagtggtt cagaccaatt gttacttccg ggcgggcagg gcgatcagcg
     9901 gcagggcggc cgcgatgagg gcgaggcccg cgagcgcggg gccgccgagg tgctcgccga
     9961 gcagggcgac gccgaggacg gcggcgaccg cgggttcggc gagcgtcagc gtcgtcgcgg
    10021 cggtggcggg ggtggtgcgc aggccgcggg cgaacagcag gtaggcgccg ccggtcgcga
    10081 ccacgccgag gtagagcgcg atgagggcgc ccgaaccggt cagcatccac gccgggccgg
    10141 cgaacggcag gaggggcagg agcgggacgg ccgcgacgcc gaacagggtc ccgacgaccg
    10201 cgcgctcctc gccgccccgc gcgaggaggg cggccgtgac cgtcgtgtac gccgcgtagg
    10261 cgaggccgga gagggcggcg agcccgatgc ccgcggggtc ggcgccggcg tccgcgccgc
    10321 cgccggcgag cagggcgcac ccggtgacgg cggctgccgt cgcgaacgtc cagcggaggc
    10381 ccggcacggt gcggcggacg gcgacgccga tgagcccggc gaacgcgggc gcggaaccga
    10441 tggtgacgac ggtcgcgatc gcgacgccgg tgcgggccgc ggccacgaag aacgcggtct
    10501 ggtagacggc gatggcgagc gcgccgagca ggacgagggg acgggcgccg cggtcggcgg
    10561 cgagccgccg caggcccgcg ccccggaccg tcccgtcccg gagcagggcg gcggaggcca
    10621 gcaggaccag cccgccgagg acgatgcgca tcgcggcgac cgacaggctg gacgcgccgt
    10681 cggcgaacgt gcgggcggtg ccggtcgtcc cccacaggga cgccgccgcg aggatgggga
    10741 gggtgcctcc gcccggcagc ccccgcccgg acggaccgga ggcgggggcg tgcccggacg
    10801 gggacgtgga cggggacgtg gacggggatg tgcgcgcgga acggccgaac gaaggcgtgc
    10861 gcagagacat ggagtactgc tcctgagctc gaatcgggag agggattcc
//
