LOCUS       NZ_CP031598            10402 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Roseovarius indicus strain DSM 26383 chromosome, complete genome.
ACCESSION   NZ_CP031598
VERSION     NZ_CP031598.1
KEYWORDS    RefSeq.
SOURCE      Roseovarius indicus
  ORGANISM  Roseovarius indicus
            Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
            Roseobacteraceae; Roseovarius.
REFERENCE   1  (bases 1 to 5445172)
  AUTHORS   Vollmers,J. and Petersen,J.
  TITLE     Genetic Globetrotter - A new plasmid hitch-hiking vast phylogenetic
            and geographic distances
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5445172)
  AUTHORS   Petersen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2018) Single Cell Genomics, Leibniz Institute
            DSMZ, Inhoffenstr. 8, Brunswick 38126, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: DSMZ_RIdsm
            Expected Final Version            :: yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RS; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008728195.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 05:52:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,061
            CDSs (total)                      :: 6,008
            Genes (coding)                    :: 5,925
            CDSs (with protein)               :: 5,925
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 24 of 83
            Pseudo Genes (incomplete)         :: 57 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2324108
            Orig. end                         :: 2334510
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031598.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="10"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5402
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1112..2419
                     /locus_tag="RIdsm_RS10775"
                     /old_locus_tag="RIdsm_02167"
     CDS             1112..2419
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815618.1"
                     /locus_tag="RIdsm_RS10775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02167"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_057819486.1"
                     /sec_met_domain="DAO (E-value: 9.5e-52, bitscore: 175.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQWNDESEPGTPYWWSVLKNDPADDPLPQDCDVLVIGAGFAGLSA
                     AIASHDCGASVVVVDKDEPGQGASTRNGGMFGAHPRLGWKALSDKFGPKVADALFAEAK
                     PALEWAKGLIDREGIDCDLQQTGRIQLAWTRQHFENQKTLARHVSEKSDVDVSIVERDG
                     LGQEIETPRYFGGLVFPEHCGLHPAKYHQGLREAVTRRGITLTGNAEVTSLERAGNRFS
                     VTTPKGTVRAEKVILCTNGYTTPLFRWHVARVFPLPSYLIATEELPANLLGHLAPGRRM
                     MVETRARHSYFRLSPDGKRVLWGGRASMRDVPVNEAAKRLRETMLSVWPDLADARISHA
                     WWGNTGFSFNHMPHVGEDRGLHYAMGFSGSGTVMAPYLGAKAAYRALGDPRGATAYADT
                     VLRRDALHPFAKPHFLKAADIWYRQVVDRVENAKAR"
     gene            complement(2423..2899)
                     /locus_tag="RIdsm_RS10780"
                     /old_locus_tag="RIdsm_02168"
     CDS             complement(2423..2899)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862534.1"
                     /locus_tag="RIdsm_RS10780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02168"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_057819544.1"
                     /transl_table=11
                     /translation="MERIDTCLIALRRILRATDTYARDLAQSAGLTAVQFRVLQLIAER
                     GRCTATEISHRMIVSQATVTALVDKLVKKGLVVREKSDTDRRQTNIILTEHGRATVDNA
                     PDALQQQFVARFEALEDWEQSMIVASLERVATMLNAANLDASPVLDSREFRNGV"
     misc_feature    2919..2935
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.42"
                     /tool="antismash"
     misc_feature    complement(2932..2945)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 18.28"
                     /tool="antismash"
     misc_feature    complement(2935..2948)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.4"
                     /tool="antismash"
     misc_feature    3014..3027
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            3124..3654
                     /gene="ectA"
                     /locus_tag="RIdsm_RS10785"
                     /old_locus_tag="RIdsm_02169"
     CDS             3124..3654
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862532.1"
                     /locus_tag="RIdsm_RS10785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02169"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_177228451.1"
                     /transl_table=11
                     /translation="MRHTLELIDKATPSLRKPVAEDGAEIWKLIKACKPLDENSMYCNL
                     IQCDHFRDTCVLAELGGKPAGWVSAYVMPNDPSTLFVWQVAVSSEARGMGLGTLMLKGL
                     LQRDVCKDVERIQTTITRSNAASWKLFRKFAKQQDARLSSQAYFGEALHFQGKHNTEYM
                     VTIDLAEEMDKAA"
     gene            3704..4996
                     /gene="ectB"
                     /locus_tag="RIdsm_RS10790"
                     /old_locus_tag="RIdsm_02170"
     CDS             3704..4996
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-364). E-value: 3.6e-74.
                     Score: 241.7. Matches aSDomain:
                     nrpspksdomains_RIdsm_RS10790_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805325.1"
                     /locus_tag="RIdsm_RS10790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02170"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_057819484.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.8e-77, bitscore:
                     259.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTDTSADKNIFERRESEARSYCRNFDTVFTKALGSELTDAHGKT
                     YIDFLAGCSSLNYGHNDPDMKAALIEHISGDGIAHGLDMYTTEKARFLEAYEEIILKPR
                     GMDHKIMMTGPTGTNAVEAAMKLARKVTGRRNIVAFSNGFHGMTMGALSLTGNAGKRGG
                     AGGGALTDVTHFPFEGALGDDVDTLAQAEMMLDNSSSGIDAPAAFIFEPIQGEGGLTAA
                     SKEWMQGVERLARKYGAMLIVDDIQSGCGRTGSYFAFEEAGIDPDMITQAKSLSGFGLP
                     FAALLIKPEHDIWKPAEHNGTFRGNTHAFVTGRVALEKFWKTDDFAKEVQEKSKILTEG
                     LQSIVDIIPGARLKGRGLMQGVDVGSGDNAGEICARAFEKGLVIETSGADDEVVKVLAP
                     LTTPEDTFRKGLDILIESAREVSTSTKMAAE"
     aSDomain        3791..4795
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RIdsm_RS10790_Aminotran_3.1"
                     /evalue="3.60E-74"
                     /label="RIdsm_RS10790_Aminotran_3.1"
                     /locus_tag="RIdsm_RS10790"
                     /protein_end="364"
                     /protein_start="29"
                     /score="241.7"
                     /tool="antismash"
                     /translation="FTKALGSELTDAHGKTYIDFLAGCSSLNYGHNDPDMKAALIEHIS
                     GDGIAHGLDMYTTEKARFLEAYEEIILKPRGMDHKIMMTGPTGTNAVEAAMKLARKVTG
                     RRNIVAFSNGFHGMTMGALSLTGNAGKRGGAGGGALTDVTHFPFEGALGDDVDTLAQAE
                     MMLDNSSSGIDAPAAFIFEPIQGEGGLTAASKEWMQGVERLARKYGAMLIVDDIQSGCG
                     RTGSYFAFEEAGIDPDMITQAKSLSGFGLPFAALLIKPEHDIWKPAEHNGTFRGNTHAF
                     VTGRVALEKFWKTDDFAKEVQEKSKILTEGLQSIVDIIPGARLKGRGLMQGVDV"
     gene            5001..5402
                     /locus_tag="RIdsm_RS10795"
                     /old_locus_tag="RIdsm_02171"
     CDS             5001..5402
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043446.1"
                     /locus_tag="RIdsm_RS10795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02171"
                     /product="ectoine synthase"
                     /protein_id="WP_057819483.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.8e-47, bitscore:
                     156.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRDFNDIVENQKDRVISDAEWSSVRMLLADDKMGFSFHITFLN
                     AGSEHTFEYKNHFESVYCMSGKGSITDIATGETHEIKPGVMYALNEHDRHILRAEEELV
                     MACVFNPPVTGTEVHREDGSYAPAEELAD"
     gene            5423..6859
                     /locus_tag="RIdsm_RS10800"
                     /old_locus_tag="RIdsm_02172"
     CDS             5423..6859
                     /EC_number="2.7.2.4"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331766.1"
                     /locus_tag="RIdsm_RS10800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02172"
                     /product="aspartate kinase"
                     /protein_id="WP_057819481.1"
                     /transl_table=11
                     /translation="MSFPKHTVEKIGGTSMSRVNELLDTLLIGEREGSDLYHRIFVVSA
                     FGGITDLLLEHKKSGRPGVYGLFANDDNDHGWLDALSSVGDAMCEAHRKVLTHPGDQNM
                     ADEFVRERIEGARSCLFDLQRLCSFGHFRLSSHMLVIRELLSGLGEAHSAFVTALLLQR
                     NGVDARFVDLSGWRDESEHTLKERIAAGLDDIDLTRELPIVTGYAQCTEGLMREYDRGY
                     SEVTFSNIAAHTGAHEAIIHKEFHLSSADPGLVGVENARKLGHTNYDVADQLANMGMEA
                     IHPNAAKILRRAGITLRVTNAFEPGDPGTLIDEEKAEHPGVEIVTGLDVVALELFEQDM
                     VGVKGYDATALEALKRHNVWIVSKTSNANNITHYIQASLKTAKRVIREIEEVYPQAEVT
                     ARAVSLVAVIGRDLTGERVLGHGLQALHEAGIDVVAAQQTPRNVDAQFVVPRDSADDAV
                     KCLHDAFVSGKSGAGLARVA"
     gene            complement(6946..7116)
                     /locus_tag="RIdsm_RS30100"
                     /old_locus_tag="RIdsm_02173"
     CDS             complement(6946..7116)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814151.1"
                     /locus_tag="RIdsm_RS30100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02173"
                     /product="hypothetical protein"
                     /protein_id="WP_177228450.1"
                     /transl_table=11
                     /translation="MGFPFDTPTWDGVSGAIFMGLGTSAPAIFSFIAIVMAILALIVGQ
                     KVESGKYRSHK"
     gene            complement(7132..8517)
                     /locus_tag="RIdsm_RS10805"
                     /old_locus_tag="RIdsm_02174"
     CDS             complement(7132..8517)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814153.1"
                     /locus_tag="RIdsm_RS10805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RIdsm_02174"
                     /product="ammonium transporter"
                     /protein_id="WP_057819479.1"
                     /transl_table=11
                     /translation="MEEQLAELAAQVAALEAKGNLTNTMFAETYYYLTIPLMIIIHAGF
                     LAYEMGASRAKNALSSGVKNILAFAFVIPAFYFFGWWVYWAFPTGFSLSAGPNGISGAE
                     YANGFALAWGDAAAYMGPNTEDQIDGVFWGAFTLFAATTASIFSGAVIERIQTVGFVIL
                     AIVLGGFSWTLAAAWGWHSDGWLIQQWGVHDFGAAGLVHAVAAFFALGVLINLGPRIGK
                     FNEDGTANNLAGHNMPFTATGLMLIVVGFWGFLMGCLVVGGESWSWGGNFTTIYGTPTN
                     LGALSFNILMGVAGGIIGAWTLTRDPFWMMSGALAGLISVASGVDIYFPSLTFIIAMAA
                     GLMLKPAAAFIESFGIDDAVGAVTVHGVMGVFGMLVLGIFASGYPALLGEGVPTVSFIG
                     QLVGTVVFVAIGFITGYGCSLILKMVGMLRVPEAAEIAGLDTVKVPAQAYPEGIMASPP
                     ASK"
     gene            8485..8748
                     /locus_tag="RIdsm_RS30360"
     CDS             8485..8748
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RIdsm_RS30360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_177228447.1"
                     /transl_table=11
                     /translation="MGRKLRELFFHVVRTSIPLVGLLTGPFGSQLVLYQCERYTNAKPT
                     QNFNVAEIEGKQAENTCVTRGKRFVRRGLSRQCGQLPGEGTD"
     gene            complement(8809..9039)
                     /locus_tag="RIdsm_RS10810"
                     /old_locus_tag="RIdsm_02175"
     CDS             complement(8809..9039)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RIdsm_RS10810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RIdsm_02175"
                     /product="hypothetical protein"
                     /protein_id="WP_057819477.1"
                     /transl_table=11
                     /translation="MSTTIVETWSGADLSQLGPVYPGVGWEVTLFVVGVAFWLLFHILQ
                     ARIEKKEFEVDEAAARSKERLERVFDEEARE"
     misc_feature    complement(9081..9096)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     misc_feature    9241..9257
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.0"
                     /tool="antismash"
     misc_feature    9365..9378
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     misc_feature    complement(9927..9940)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.9"
                     /tool="antismash"
ORIGIN
        1 tcatctgtaa cgcgggcacg cttggcatcc tgatcccgcc gtcgatcgtg atggtggtct
       61 atgccgcggc ggtcgaggtg tcggtcggcc ggatgttcct ggccggggtc attccaggtc
      121 ttctggcggg tttcatgctg atgacggcca tctacatcgt ggcccgcgtg cgcaacatgc
      181 ccaagggcga ctggcagggc tggggcgaga tctttgcctc cgggcgcgat gcggcctggg
      241 gccttttcct gatcgtcatc atccttggcg gcatctatgg cggcatcttc accccgaccg
      301 aggccgcggc ggtggcggcg gtctactcgt tcttcatcgc ctgtttcgtc tacaaggaca
      361 tggggccgct cgcggtcggc aaggagcagg gcggcaatct gagcctcctg cgcaagccgt
      421 acgcaatcgt cacggccttc ttccaccccg acaccaagca cacgctcttc gaggcgggca
      481 agctgaccgt gacgctgctt ttcgtcatcg ccaacgcgct gatcctcaag cacgtgctga
      541 ccgatgagca gatcccgcag cagatcgccg gcgccatgct caacgcgggt ttcgggcctg
      601 tcatgttcct gatcgtcgtc aacgtgatcc tgctgatcgg cggccagttc atggagcctt
      661 cgggccttct cgtgattgtt gcgccgctgg tgttcccgat cgccatggag cttgggatcg
      721 accccattca cctcggcatc atcatggtgg tgaacatgga gatcgggatg attaccccgc
      781 ctgtcggtct caacctcttc gtgacctcgg gcgtggcggg catgccgatg atgagcgtcg
      841 tgcgcgcggc gctgccgttc ctcgcggtgc tcttcgtctt cctgatcatg atcacctaca
      901 ttccgtggct ctcgacggtg ttgccgaaca ccttcatggg ccccgagatc gtcacgcagt
      961 aggaagctga aacggttcgg gcgcgtagga tttatgcgcg cccgaccacc gggccgtctg
     1021 gcccgcattt gccctccggt ttgtcgcaga tgtgtcatgg acaagccgcc tgacccggca
     1081 gatagtcggc ccggtcaacg agggaacacc catgcagtgg aatgacgaat ccgaacccgg
     1141 cactccgtat tggtggagcg ttctgaagaa cgacccggct gacgatccgt tgccgcagga
     1201 ttgcgacgtg ctggtgatcg gcgcgggctt cgccggtctg tcggcggcga tcgcctccca
     1261 tgattgcggc gccagcgttg tggttgtcga caaggacgaa ccggggcagg gcgcctccac
     1321 ccgcaacggc ggcatgttcg gtgcccatcc ccggctggga tggaaggcgc tttccgacaa
     1381 gttcggccca aaggtggccg atgcgctctt tgccgaggcg aagccggcgc ttgaatgggc
     1441 gaaggggctg atcgaccgcg aaggcatcga ttgcgacctg caacaaacgg gtcgcatcca
     1501 gcttgcctgg acaaggcagc atttcgaaaa ccagaaaacg ctcgcccgcc atgtctcgga
     1561 gaagagcgac gtcgacgtct cgatcgtcga gcgtgacggg ctggggcagg aaatagagac
     1621 gccccgctat ttcggcgggc tggtcttccc cgaacattgc ggacttcacc cggccaagta
     1681 ccaccagggg ctgcgcgagg ctgtcacccg ccgcggcatc acgctcaccg gcaacgccga
     1741 agtgacgtcg ctcgagcgcg ccggcaaccg gttctccgtc accacgccaa agggcaccgt
     1801 tcgggcggaa aaggtcatcc tctgcaccaa cggctacacc acgccgcttt tccgctggca
     1861 cgtggcgcgg gttttcccgc tgccctccta cctgatcgca accgaggaat tgccggccaa
     1921 cctgctgggg catctcgccc cggggcggcg gatgatggtc gaaacccgcg cccggcacag
     1981 ctatttccgc ctgtcacctg atggcaagcg cgtgctctgg ggcgggcggg cctccatgcg
     2041 tgacgtcccg gtcaacgagg ccgccaaacg cctgcgcgag acgatgctca gcgtctggcc
     2101 ggacctcgcc gatgccagga tcagccacgc ctggtggggc aatacgggct tcagcttcaa
     2161 ccacatgccc catgtgggcg aggatcgtgg gctgcattac gcaatgggct tctcgggatc
     2221 gggcaccgtc atggcgccct atctgggcgc caaggcagct tatcgcgccc tgggcgatcc
     2281 gcgcggggcc acggcctatg ccgataccgt cttgcgccgg gatgcccttc atcccttcgc
     2341 caagccgcat ttcctaaagg ccgcggatat ctggtatcgg caggtggtcg accgggtgga
     2401 gaacgcgaaa gcgcgctgaa cgtcagacgc cgttccggaa ttcccgcgag tcgagcaccg
     2461 gcgatgcgtc gagattggcg gcattgagca tcgtcgcgac ccgctcgagc gaggccacga
     2521 tcatcgactg ctcccagtcc tcgagcgcct cgaaccgggc gacgaactgc tgctgaagcg
     2581 catcgggcgc gttgtcgact gtcgcccgcc cgtgctctgt caggatgatg ttggtctgcc
     2641 gccggtcggt gtcagacttc tcacgtacca ccagcccctt tttcacaagc ttgtccacca
     2701 gcgccgtcac cgtcgcctgg gacacgatca tccggtgcga tatctcggtg gcggtgcatc
     2761 gcccccgttc ggcgatgagc tgcagcactc ggaattgcac cgctgtcaga ccggccgact
     2821 gggccaggtc acgggcatag gtgtcggttg cgcgaaggat tctgcgcagg gcgattaggc
     2881 aggtatcgat ccgttccatg gccatctccg caaggatttt gtttcatagt cacataataa
     2941 taatatttcg gcaatccacg aaaatgcttc ggtcgatgaa ggaaacttta ggtttttaag
     3001 gcataaaacc gctattttgc actaaaaatg cgacatattg ctttggtatg ctttgcatct
     3061 tgaagtttgt ggtgcggcaa actactttag tatgcaaagg aaaactgagg agacagcaga
     3121 ccgatgcgac acacgctgga actgatcgac aaagcgacgc cctcgttgcg aaaacccgtg
     3181 gccgaggacg gcgcggaaat ctggaagctg atcaaggcat gcaaaccgct cgacgagaac
     3241 tcgatgtatt gcaaccttat ccagtgcgac catttccgcg acacttgtgt tctggctgaa
     3301 ctgggtggca agccggcggg ctgggtttcg gcctacgtca tgccgaacga tccctccacg
     3361 ctgttcgtat ggcaggtcgc tgtttcttcc gaagcgcgcg gtatgggcct tggcacgctg
     3421 atgctcaagg gccttctcca gcgcgacgta tgcaaagatg tcgagcgtat ccaaacgacc
     3481 atcacgcgct cgaacgccgc ctcgtggaag ctgtttcgca aattcgcgaa acagcaggac
     3541 gcgcggctct cctcgcaggc ctatttcggt gaggccctgc acttccaagg aaaacacaac
     3601 accgaataca tggtcaccat cgacctcgcg gaagaaatgg acaaggcagc ctgaacgggc
     3661 tgcccgcctt ccgcttcaaa tccatcaaac caaaggacac gccatgccga ctgacacgtc
     3721 agccgacaag aacatatttg aacgccgcga atctgaagcg cgcagctatt gccgcaactt
     3781 cgatacggta ttcacaaagg ccttggggtc cgaacttacc gacgcccacg gcaaaaccta
     3841 catcgacttc ctggccgggt gctcgtcgct caactacggg cacaacgatc ccgacatgaa
     3901 agccgccctg atcgagcata tctcgggcga cgggatcgcg cacggcctcg acatgtacac
     3961 gaccgaaaag gcgcgcttcc tcgaagcgta cgaggagatc atcctcaagc cgcgtggcat
     4021 ggaccacaag atcatgatga ccggcccgac cggcacaaac gcggtcgagg cagcaatgaa
     4081 gctggcccgc aaggttaccg gccgccgcaa catcgtggcc ttctccaacg gcttccatgg
     4141 catgaccatg ggcgcactgt cgctgaccgg taacgccggc aagcgtggtg gcgccggtgg
     4201 cggtgccctg accgatgtca cgcacttccc cttcgaaggc gcgctcggcg acgacgtcga
     4261 tacgctggcg caggccgaaa tgatgctcga caactcctcg tccggcatcg atgcacctgc
     4321 cgcgttcatc ttcgagccga tccagggcga gggcggtctt acagccgcgt cgaaggaatg
     4381 gatgcagggt gtcgagcgcc ttgcgcgtaa gtatggcgcc atgctgatcg ttgacgacat
     4441 ccagtcgggc tgcggccgca cgggcagcta cttcgccttc gaggaggcgg gtatcgaccc
     4501 tgacatgatc acgcaggcga aatcgctgtc gggcttcggc ctgccgttcg ccgcgctgct
     4561 gatcaagccg gaacacgata tctggaagcc cgccgagcat aacggcacct tccgtggcaa
     4621 cacccatgct ttcgtgaccg gccgggttgc gctcgaaaag ttctggaaga ccgatgactt
     4681 cgccaaggaa gtccaggaga agagcaagat cctgaccgaa ggtctccagt ccatcgtcga
     4741 catcatcccc ggcgcgcgcc tcaagggccg tgggctgatg caaggcgtcg acgtgggctc
     4801 cggcgacaat gccggcgaga tctgcgcacg cgccttcgag aagggcctcg tgatcgagac
     4861 gtcgggtgcc gacgacgaag tcgtcaaggt gctcgcgccc ctcacaacgc cggaagacac
     4921 cttccgcaag ggcctcgaca tcctgatcga gtcggcacgc gaagtttcga cctccaccaa
     4981 gatggcagcg gagtaacaca atgattgtca gagatttcaa cgatatcgtc gaaaaccaga
     5041 aagaccgcgt catcagtgac gcagaatggt cgtcggtcag gatgctcctt gccgatgaca
     5101 agatggggtt ttcgttccac attacgttcc tgaacgcggg ctcggaacac acgttcgagt
     5161 acaagaacca cttcgagagc gtgtactgca tgtccggcaa ggggtcgatc accgacatcg
     5221 ccaccgggga aacacacgag attaagcctg gcgtgatgta cgcgctgaac gagcacgacc
     5281 gccacatcct gcgggccgag gaagagcttg tcatggcctg tgtgttcaac ccgccggtga
     5341 cgggcaccga ggtacaccgc gaagacggct cctatgcccc ggctgaagaa ttggcggact
     5401 gaccgccgaa aggaatgatg tcatgagttt tcccaagcat accgtcgaaa agatcggcgg
     5461 cacctcgatg agtcgcgtga acgagttgct cgacacgctt ctgatcgggg agcgcgaagg
     5521 atccgatctc taccaccgca tcttcgtcgt ctcggccttt ggcggcatca ccgaccttct
     5581 gctcgagcac aagaaatcgg gccgcccggg cgtctatggc ctctttgcca atgacgacaa
     5641 tgatcatgga tggctcgacg cgctctcctc ggttggcgac gccatgtgcg aggcgcaccg
     5701 gaaggtgctt acccacccgg gtgaccagaa catggccgac gagttcgttc gcgaacggat
     5761 cgagggcgcg cgttcgtgcc tcttcgacct gcagcgcctg tgctccttcg ggcatttccg
     5821 cctgtcgagc cacatgctgg tcatccgcga gcttctgtcg ggcctgggcg aggcgcattc
     5881 ggcctttgtc accgcgctgc tgctgcagcg caatggcgtc gatgcccgct tcgttgacct
     5941 cagcggttgg cgcgacgagt ccgagcacac gctgaaagag cgtatcgcag ccggtctcga
     6001 cgatatcgac ctgacgcgcg aactgcccat cgtcaccggc tacgcccagt gcaccgaagg
     6061 gctgatgcgc gaatacgacc gtggctattc cgaggtgacc ttctcgaaca tcgccgccca
     6121 cacgggcgcg cacgaggcga tcatccacaa ggaattccac ctgtcctcgg ccgatccggg
     6181 ccttgtgggc gtggaaaacg cacgcaagct gggtcacacc aactacgacg tggccgacca
     6241 gttggcgaat atggggatgg aggcgatcca cccgaacgca gccaagatcc tgcgccgggc
     6301 cggcatcacg ctgcgcgtga ccaacgcgtt cgagccgggc gacccgggca cgctgatcga
     6361 cgaggagaag gccgagcatc ccggcgtgga gatcgtcacc ggtctcgatg tcgtggccct
     6421 cgagctcttc gaacaggaca tggtcggcgt caaaggttac gacgccaccg cgcttgaggc
     6481 gctcaagcgt cacaacgtgt ggatcgtgtc gaaaacctcg aacgccaaca acatcacgca
     6541 ctatatccag gcctcgctca agacggccaa gcgcgtgatc cgcgagatcg aggaggtcta
     6601 tccgcaggca gaagtcacgg cacgggccgt atcgcttgtg gcggtcatcg gccgcgacct
     6661 gacgggcgag cgggtgcttg gccacgggct tcaggcgctg cacgaggcgg gtatcgacgt
     6721 tgtcgccgcc cagcagacgc cgcgcaatgt cgacgcccag ttcgtcgtgc cgcgcgattc
     6781 cgctgacgat gcggtgaagt gcctgcacga cgcgttcgtc tcgggaaaga gcggggcggg
     6841 gctggcgcgg gtggcgtgac cgctgccgca tctgctccgc tctaagaagg cgccccgctg
     6901 gatgatggcc agcggggcgt ctcatttttc agctatgacg cggccttact tgtggctgcg
     6961 atatttgccc gactcgacct tttggccgac gatcagcgcc aggatggcca tgacgatcgc
     7021 gatgaacgaa aagatcgccg gggccgaggt tccaagcccc atgaagatcg cgccggagac
     7081 gccatcccat gtcggtgtgt caaagggaaa tcccattctt aagtcctcct gttacttgga
     7141 tgccgggggc gatgccatga tgccctcggg ataggcctgt gccggcacct tgacggtgtc
     7201 gagaccggcg atttcagcag cttccggcac gcgcagcatg ccaaccatct tcaggatgag
     7261 cgaacatccg tacccggtga tgaacccgat cgcgacgaac acgaccgtgc ccacaagctg
     7321 accgatgaag ctgaccgtcg gtacgccttc gcccagaagg gcagggtagc cgctggcgaa
     7381 gatgccaaga accagcatgc cgaagacacc cattacgccg tgaaccgtga cagcgcccac
     7441 cgcgtcgtcg atgccgaagc tctcgatgaa ggccgccgcc ggtttcagca tcagccccgc
     7501 cgccatggcg atgatgaagg tgagcgaggg gaagtagatg tcgacccccg aggccaccga
     7561 tatcagaccg gccagcgcgc ccgacatcat ccagaacgga tcgcgtgtca gtgtccaggc
     7621 gccgatgatg ccgcccgcga cacccatcag gatgttgaag ctgagcgcgc cgaggttggt
     7681 gggcgtgcca tagatggtgg tgaagttgcc gccccacgac cagctttcgc cgccgaccac
     7741 gagacatccc atcaggaagc cccagaaacc cacgacgatc agcatcaggc ccgtcgccgt
     7801 gaagggcatg ttgtgcccgg cgaggttgtt ggccgtgccg tcctcgttga acttgccgat
     7861 ccgcgggccg agattgatca gcacgccaag ggcaaagaac gccgcgaccg cgtgcacaag
     7921 gcccgccgcg ccgaagtcat gaacgcccca ttgctggatc agccaaccgt ccgaatgcca
     7981 accccatgcg gccgccagcg tccagctgaa cccgcccagc acgatcgcca ggatcacgaa
     8041 gcccaccgtc tggatgcgct cgatgaccgc gcccgagaag atcgaggccg tcgttgccgc
     8101 aaacagcgtg aaggcgcccc agaagacgcc gtcgatctgg tcttccgtgt tcgggcccat
     8161 ataggccgcc gcgtcgcccc aggcgagcgc gaagccgttg gcgtattccg cgccggagat
     8221 cccgtttggc ccggccgaaa gagagaaccc cgtggggaag gcccagtaga cccaccagcc
     8281 gaagaagtag aaagccggga tcacgaaggc gaaggccaga atgttcttca cgcccgaaga
     8341 aagggcgttc ttggcgcgcg aggcgcccat ttcataggcc aggaaccctg catgaatgat
     8401 gatcatcagt ggaatggtta agtaatagta ggtttctgcg aacatcgtgt tcgtcagatt
     8461 ccccttcgct tccagggcgg ccacttgggc cgcaagctcc gcgagctgtt cttccatgtc
     8521 gttcgtacct ccataccgtt ggtgggcttg cttaccggcc cttttggttc acaattggtt
     8581 ctttatcagt gtgaacgcta caccaatgct aagccaacac agaatttcaa tgttgctgaa
     8641 atagagggca aacaggctga aaatacgtgc gttacccgtg gtaaacgctt tgttcgacgc
     8701 ggattatccc ggcaatgcgg gcagttgccg ggtgagggga ccgattgaac caaaaacgcc
     8761 ggcccagggg gccggcgctt gctttttggg cgataactcg aggtgcgcct attctcgcgc
     8821 ctcctcgtca aatactcgtt cgaggcgctc cttgctgcgc gccgcggcct cgtcgacctc
     8881 gaactccttc ttctcgatcc gggcctgaag gatgtggaac agcagccaga aggcgacacc
     8941 gacaacaaac agcgtcacct cccatccgac gcccggatag acagggccaa gttgcgacag
     9001 gtccgcgccg gaccaggttt cgacgatcgt ggtagacatg attgcgtcct cctcaggcca
     9061 gcagtccgag gcgcttggcc tcttcgatat cggcttccga cacggcgatc gcgtcggcct
     9121 cctctgacag ttcctcgctc atgtcgaggc ccaccagctc caccgcgggc ggcacgcgca
     9181 gcatgcccat gccctgcagg atcttcgaga cgatccaggc cgggataaag cccagcacga
     9241 tgaacatgat gcaggcaccg gcaagctggc cccatggcgt gatcacggcc acctcttcat
     9301 ggtagcccgc cgtggccggg tggccccaca gaacgaagcc ggcaatgatc aggccgatga
     9361 aacccgcata gccgtgcacc gcaaccgcgc cgaccggatc gtcgatcttg aacgtccgct
     9421 cgacccagaa atgcagcttg taggcacagt aggcgccaac cccgccgatc agcatcgcct
     9481 cgatcgggtg gtagaggtca ttgccggccg acgccgtgat gacccctgca aggcctgccg
     9541 agtaggtcca gaacgcctcg ccgcgcgaga cgacatagcc catcagcaag ccgcccgaca
     9601 gcgacatcag gaagttgaag acgatggccg acagggtggt gggcgtcagg tagatcgtcg
     9661 ttgcggtgaa cgtgccttcc gcggccccga tggcgcccga gccgatgatc ggcacgttgc
     9721 aggccacgta gaagccccag aagccgcagt agatcaggaa aagcccaatc gtcaccagcc
     9781 acttgttgtg cgggttgatg ttgcgcgggg tgccgtcggg ggcgaacttg ccgatccttg
     9841 gccccagcac gcagatcacg ccaagcgcga aaccgcccgc gatcgcatgg atcacgcccg
     9901 acgcataggc gtcgtgatag cccagctgcg tgaccatcca gccatccgga tgccagcccc
     9961 aggccgcgtc gatgatccag gtgaacgagc cgatgacgac cgcgaggatc cagaaggccg
    10021 agggccggat ccgttcgatc gtggcacccg agacgatcga ggccgcggtc cagctgaaca
    10081 gcaggaatgc agcccagaac acgccgttca ggcgcgccca gtagttgttg ccgagatcga
    10141 cgccatagcc cttggcctgc gggtcgttga tgcccggccc gccaaggtgc gtacccatca
    10201 gctcgcccca cggcacggcg ttgtccgatt gggtaaggcc gccggtgatg cccgggccgt
    10261 tcgggaaggc gaagtagatc caccagccga agaagaagaa ggtcacggtc accagcggga
    10321 tcagcatggt gttcttcatc agggtgtgga tgtggttctt gtgacgtgtt gcgccgacct
    10381 cgtacatgca gaacccgacg tg
//
