GCF_008728085.1 - 37 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_VYTZ01000001.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	PKS-like,NRPS	60,728	122,824	Low	naphthyridinomycin	NRPS:Type I

NZ_VYTZ01000011.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	T1PKS	1	44,091	Low	X-14547	PKS
Region 3.2	T1PKS	89,280	142,221	Low	Legonmycin A/Legonmycin B/Legonindolizine A/Legonindolizine B	NRPS:Type I+other:other

NZ_VYTZ01000013.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	NI-siderophore	135,429	166,764

NZ_VYTZ01000014.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	NRPS,t3nrps-iterative,blactam,PKS-like,NRPS-like,butyrolactone	1	95,858	Low	pyridomycin	NRPS:Type I+PKS
Region 6.2	lanthipeptide-class-i	158,029	183,229

NZ_VYTZ01000015.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	terpene	90,304	111,236	Low	isorenieratene	terpene

NZ_VYTZ01000016.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	terpene	84,536	107,068
Region 8.2	T3PKS,NRPS	119,457	194,694	Medium	feglymycin	NRPS:Type I

NZ_VYTZ01000017.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	phenazine	59,463	79,951	Medium	phenazine SA/phenazine SB/phenazine SC	other:other

NZ_VYTZ01000018.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	lanthipeptide-class-iv	6,440	29,223	Low	labyrinthopeptin A2/labyrinthopeptin A1/labyrinthopeptin A3	ribosomal:RiPP:Lanthipeptide

NZ_VYTZ01000002.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	lanthipeptide-class-ii	515,290	538,166
Region 12.2	terpene	565,166	586,134

NZ_VYTZ01000020.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 13.1	redox-cofactor	34,034	56,882
Region 13.2	T1PKS	58,958	120,244	Medium	salinilactam	PKS

NZ_VYTZ01000025.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	terpene-precursor,T1PKS,NRPS,NRPS-like	1	73,029	Low	aurachin C/aurachin D/aurachin SS	PKS+terpene

NZ_VYTZ01000003.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	lanthipeptide-class-i	63,778	87,925
Region 23.2	terpene-precursor	286,940	308,007

NZ_VYTZ01000030.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 24.1	T1PKS	1	10,302	Low	desulfoclethramycin/clethramycin	other:other

NZ_VYTZ01000033.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 27.1	T1PKS	1	8,331

NZ_VYTZ01000034.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 28.1	NRPS	1	7,501

NZ_VYTZ01000004.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 34.1	NI-siderophore	325,848	356,196	Low	FW0622	other:other
Region 34.2	RRE-containing	631,467	652,591

NZ_VYTZ01000005.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 45.1	T1PKS	1	29,272
Region 45.2	terpene	436,503	461,760	Low	hopene	terpene
Region 45.3	terpene-precursor	462,361	483,428
Region 45.4	azole-containing-RiPP,thioamitides	600,749	630,916

NZ_VYTZ01000006.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 50.1	T3PKS,lanthipeptide-class-iii	109,423	150,511	High	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone/2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	PKS:Type III
Region 50.2	T1PKS,other	236,548	327,175	Medium	maduropeptin	PKS:Iterative type I
Region 50.3	NRPS	410,391	468,763

NZ_VYTZ01000007.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 51.1	terpene	128,328	153,210	High	geosmin	terpene:Sesquiterpene
Region 51.2	NAPAA	156,414	190,478
Region 51.3	NRPS	301,558	347,092

NZ_VYTZ01000008.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 52.1	lanthipeptide-class-i	33,423	58,545	Low	tobramycin	saccharide
Region 52.2	RiPP-like	177,179	187,448
Region 52.3	RRE-containing	224,420	245,541

NZ_VYTZ01000009.1 (Microbispora cellulosiformans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 53.1	T1PKS	131,384	179,195

No secondary metabolite regions were found in these records:: NZ_VYTZ01000010.1 (Microbispora cellulosiformans); NZ_VYTZ01000012.1 (Microbispora cellulosiformans); NZ_VYTZ01000019.1 (Microbispora cellulosiformans); NZ_VYTZ01000021.1 (Microbispora cellulosiformans); NZ_VYTZ01000022.1 (Microbispora cellulosiformans); NZ_VYTZ01000023.1 (Microbispora cellulosiformans); NZ_VYTZ01000024.1 (Microbispora cellulosiformans); NZ_VYTZ01000026.1 (Microbispora cellulosiformans); NZ_VYTZ01000027.1 (Microbispora cellulosiformans); NZ_VYTZ01000028.1 (Microbispora cellulosiformans); NZ_VYTZ01000029.1 (Microbispora cellulosiformans); NZ_VYTZ01000031.1 (Microbispora cellulosiformans); NZ_VYTZ01000032.1 (Microbispora cellulosiformans); NZ_VYTZ01000035.1 (Microbispora cellulosiformans); NZ_VYTZ01000036.1 (Microbispora cellulosiformans); NZ_VYTZ01000037.1 (Microbispora cellulosiformans); NZ_VYTZ01000038.1 (Microbispora cellulosiformans); NZ_VYTZ01000039.1 (Microbispora cellulosiformans); NZ_VYTZ01000040.1 (Microbispora cellulosiformans); NZ_VYTZ01000041.1 (Microbispora cellulosiformans); NZ_VYTZ01000042.1 (Microbispora cellulosiformans); NZ_VYTZ01000043.1 (Microbispora cellulosiformans); NZ_VYTZ01000044.1 (Microbispora cellulosiformans); NZ_VYTZ01000045.1 (Microbispora cellulosiformans); NZ_VYTZ01000046.1 (Microbispora cellulosiformans); NZ_VYTZ01000047.1 (Microbispora cellulosiformans); NZ_VYTZ01000048.1 (Microbispora cellulosiformans); NZ_VYTZ01000049.1 (Microbispora cellulosiformans); NZ_VYTZ01000050.1 (Microbispora cellulosiformans); NZ_VYTZ01000051.1 (Microbispora cellulosiformans); NZ_VYTZ01000052.1 (Microbispora cellulosiformans); NZ_VYTZ01000053.1 (Microbispora cellulosiformans)

Compact view

NZ_VYTZ01000001.1 - Region 1 - NRPS,PKS-like

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Location: 60,728 - 122,824 nt. (total: 62,097 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase oxygenase

F5972_RS00220: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

F5972_RS00250: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

F5972_RS00330: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

F5972_RS00335: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

Transferases: aminotransferase methyltransferase transketolase

F5972_RS00255: methyltransferase

smcogs: SMCOG1248: methyltransferase

F5972_RS00370: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

F5972_RS00375: transketolase

smcogs: SMCOG1204: transketolase

Hydrolases: esterase

F5972_RS00295: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

F5972_RS00355: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Isomerases: isomerase

F5972_RS00230: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

F5972_RS00275: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

F5972_RS00280: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

F5972_RS00285: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS00290: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS00300: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS00310: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS00320: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS00380: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.93	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.88	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.87	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.84	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.83	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.83	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.82	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.81	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.80	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001240.4	0.78	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.74	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001240.4	0.72	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 21.84 out of 21.84

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.25 out of 29.72

Confidence: medium

Additional weak hits (15):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.48 out of 26.85

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.70 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.98 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.77 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.96 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.60 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.16 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.42 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.72 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.16 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.81 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.09 out of 26.06

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.78 out of 20.01

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 60727 - 122824:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Ala - Ala) + (NH2) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 60727 - 101738:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Ala - Ala) + (NH2) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 61866 - 122824:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Ala - Ala) + (NH2) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS00285: Ala - Ala - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (520..942): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEANTNLAGENNLYGPTEDTTYSTH
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1639..2078): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEANTNLAGENNLYGPTEDTTYSTH
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2794..3193): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEANTNLAGENNLYGPTEDTTYSTH
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

F5972_RS00290: NH2

CAL_domain (150..445): NH2

minowa: NH2

Prediction, score:

NH2: 127.2
Acetyl-CoA: 38.6
fatty_acid: 36.1
AHBA: 10.3
shikimic_acid: 0.0

F5972_RS00300: X

AMP-binding (487..802): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AAVSGPAHLADAVACVG----------NTVFVGR
Nearest Stachelhaus code(s):: 6-clTrp DAAMCGGVAK (21% 8Å match)
Stachelhaus code match:: 60% (weak)

F5972_RS00310: X

AMP-binding (494..891): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AARAFDAAGGGWDGVVAGEFNEYGPTEATVWASV
Nearest Stachelhaus code(s):: 4,5-dhArg DAGDIAEVWK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

F5972_RS00320: X

AMP-binding (263..650): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARMFDGAAGGWHSSVGGELNDYGPTECAVWATA
Nearest Stachelhaus code(s):: dohmTyr DGGHAGDVWK (85% 8Å match); mTyr or ohdmTyr DGSHSGDVWK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
MexT	Global virulence regulator
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000011.1 - Region 1 - T1PKS

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Location: 1 - 44,091 nt. (total: 44,091 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

F5972_RS26945: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

F5972_RS26955: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

F5972_RS27010: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: PPTase acylase

F5972_RS26925: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS26930: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS26960: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Ligases: synthase

F5972_RS37495: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

F5972_RS26995: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000067.5	0.62	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000067.5	0.62	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (13):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.10 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.48 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.21 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.60 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.30 out of 18.57

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.74 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.03 out of 30.22

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.85 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.27 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 44091:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - mal) + (ohmal) + (ccmal - ccmal)
Non-colinear ordering:: F5972_RS37495 + F5972_RS26930 + F5972_RS26925

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS26925: mal - mal

PKS_AT (561..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.9
inactive: 118.9
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 58.1
Isobutyryl-CoA: 26.3
Propionyl-CoA: 26.0
Ethylmalonyl-CoA: 21.5
trans-1,2-CPDA: 21.2
fatty_acid: 20.9
Benzoyl-CoA: 20.7
Acetyl-CoA: 18.5
CHC-CoA: 16.0
2-Methylbutyryl-CoA: 10.0
3-Methylbutyryl-CoA: 3.7

PKS_AT (2251..2538): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.7
inactive: 102.9
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 54.2
Acetyl-CoA: 22.7
Propionyl-CoA: 22.5
Isobutyryl-CoA: 22.1
fatty_acid: 20.0
Ethylmalonyl-CoA: 18.3
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 17.5
CHC-CoA: 13.9
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.5

F5972_RS26930: mal

PKS_AT (559..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.3
inactive: 97.7
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 63.7
Isobutyryl-CoA: 41.1
Propionyl-CoA: 31.4
Ethylmalonyl-CoA: 30.5
2-Methylbutyryl-CoA: 27.1
Acetyl-CoA: 21.9
trans-1,2-CPDA: 17.3
Benzoyl-CoA: 16.7
fatty_acid: 14.8
3-Methylbutyryl-CoA: 2.8
CHC-CoA: 2.1

F5972_RS37495: pk - mal

PKS_AT (563..860): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 228.1
Methylmalonyl-CoA: 227.0
Methoxymalonyl-CoA: 186.7
2-Methylbutyryl-CoA: 160.0
trans-1,2-CPDA: 144.1
Propionyl-CoA: 116.3
CHC-CoA: 106.1
Malonyl-CoA: 103.2
Isobutyryl-CoA: 93.0
Benzoyl-CoA: 70.0
Acetyl-CoA: 65.7
fatty_acid: 51.7
3-Methylbutyryl-CoA: 39.9
inactive: 30.2

PKS_AT (2324..2614): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.2
inactive: 99.4
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 53.1
Acetyl-CoA: 24.4
Propionyl-CoA: 22.1
Isobutyryl-CoA: 18.9
Ethylmalonyl-CoA: 18.0
Benzoyl-CoA: 16.6
fatty_acid: 16.4
trans-1,2-CPDA: 13.3
CHC-CoA: 10.3
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.5

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_VYTZ01000011.1 - Region 2 - T1PKS

Download region GenBank file

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Location: 89,280 - 142,221 nt. (total: 52,942 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer

F5972_RS27275: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS27345: flavodoxin

smcogs: SMCOG1134: flavodoxin

Lyases: decarboxylase

F5972_RS27295: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

F5972_RS27305: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

F5972_RS27310: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS27315: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

F5972_RS27365: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002499.2	0.54	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0001137.5	0.50	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000056.3	0.48	PKS	esperamicin	Actinomadura verrucosospora
BGC0001868.4	0.45	other (phenazine)	iodinin	Xenorhabdus szentirmaii DSM 16338
BGC0002294.3	0.45	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000043.5	0.44	PKS	curacin A	Moorea producens 19L
BGC0000207.5	0.44	PKS	chartreusin	Streptomyces chartreusis
BGC0002666.2	0.43	NRPS, other (other)	Legonmycin A, Legonmycin B, Legonindolizine A, Legonindolizine B	Streptomyces sp. MA37

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002499.2	0.54	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0001137.5	0.50	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000056.3	0.48	PKS	esperamicin	Actinomadura verrucosospora
BGC0001868.4	0.45	other (phenazine)	iodinin	Xenorhabdus szentirmaii DSM 16338
BGC0002294.3	0.45	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000043.5	0.44	PKS	curacin A	Moorea producens 19L
BGC0000207.5	0.44	PKS	chartreusin	Streptomyces chartreusis
BGC0002666.2	0.43	NRPS, other (other)	Legonmycin A, Legonmycin B, Legonindolizine A, Legonindolizine B	Streptomyces sp. MA37

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.26 out of 19.26

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.67 out of 20.97

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

Additional weak hits (8):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.96 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.27 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.47 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.94 out of 27.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.42 out of 26.12

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.13 out of 23.43

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 89279 - 142221:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS27315: mal

PKS_AT (1487..1791): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.6
inactive: 114.3
Methoxymalonyl-CoA: 78.9
Methylmalonyl-CoA: 57.0
Propionyl-CoA: 42.1
Isobutyryl-CoA: 36.2
Benzoyl-CoA: 28.6
Acetyl-CoA: 27.9
Ethylmalonyl-CoA: 27.1
fatty_acid: 9.7
trans-1,2-CPDA: 9.0
2-Methylbutyryl-CoA: 8.0
CHC-CoA: 6.9
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
CelR	Cellobiose uptake repressor

NZ_VYTZ01000013.1 - Region 1 - NI-siderophore

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Location: 135,429 - 166,764 nt. (total: 31,336 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

F5972_RS29375: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: decarboxylase

F5972_RS29350: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.52	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0001408.5	0.49	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0002527.2	0.48	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0000947.5	0.43	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000943.5	0.38	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002138.2	0.37	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0002313.3	0.33	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000121.3	0.31	PKS	Pestheic Acid, RES-1214-2	Pestalotiopsis fici
BGC0002466.3	0.30	NRPS	peucechelin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0000069.5	0.26	PKS	gephyronic acid	Archangium violaceum Cb vi76

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.52	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0001408.5	0.49	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0002527.2	0.48	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0000947.5	0.43	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000943.5	0.38	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002138.2	0.37	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0002313.3	0.33	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000121.3	0.31	PKS	Pestheic Acid, RES-1214-2	Pestalotiopsis fici
BGC0002466.3	0.30	NRPS	peucechelin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0000069.5	0.26	PKS	gephyronic acid	Archangium violaceum Cb vi76

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 22.22 out of 23.05

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.86 out of 36.53

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.17 out of 25.31

Confidence: medium

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.39 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.77 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.70 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.41 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.95 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.98 out of 35.63

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
DmdR1	Iron(II)-dependent repressor
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000014.1 - Region 1 - NRPS,NRPS-like,PKS-like,blactam,butyrolactone,t3nrps-iterative

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Location: 1 - 95,858 nt. (total: 95,858 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)
blactam: (BLS or CAS or tabtoxin)
PKS-like: (t2pks2 or ksIII)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

F5972_RS29640: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

F5972_RS29665: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

F5972_RS29710: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS29770: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS29790: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS29800: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

F5972_RS29805: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

F5972_RS29820: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS29860: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

F5972_RS29875: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: aminotransferase transferase

F5972_RS29620: aminotransferase

smcogs: SMCOG1019: aminotransferase

F5972_RS29625: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

F5972_RS29700: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

F5972_RS29660: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: decarboxylase hydratase lyase

F5972_RS29635: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

F5972_RS29655: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

F5972_RS29675: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

F5972_RS29690: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

F5972_RS29775: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

F5972_RS29565: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS29745: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

F5972_RS29750: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

F5972_RS29780: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS29845: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	1.37	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	1.35	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0002276.2	1.09	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000850.5	0.95	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000846.5	0.84	other (other)	tabtoxin	Pseudomonas syringae
BGC0001223.5	0.68	other (other)	hydroxysporine	uncultured bacterium
BGC0000894.4	0.68	other (other)	citrinin	Monascus aurantiacus
BGC0002081.3	0.66	PKS	streptoketide A, streptoketide B, streptoketide C, S2502, S2507, UWM5	Streptomyces sp. Tue6314
BGC0001909.3	0.64	PKS	strobilurin A	Strobilurus tenacellus
BGC0001622.5	0.63	PKS	isatropolone A, isotropolone B, isotropolone C	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	0.75	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002276.2	0.73	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 20.47 out of 20.47

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.83 out of 20.97

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.82 out of 21.84

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.36 out of 19.26

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 23.04 out of 27.55

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

Additional weak hits (34):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.27 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.30 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.56 out of 20.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.42 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.12 out of 28.14

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.67 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.04 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.33 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.99 out of 27.78

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.14 out of 19.26

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.51 out of 20.97

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.56 out of 39.62

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.18 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.96 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.82 out of 21.55

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.67 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.58 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.82 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.39 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.51 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.96 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.93 out of 21.55

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.13 out of 36.53

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.48 out of 27.04

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.45 out of 36.54

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.54 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.22 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.77 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.86 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.16 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 95858:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Phe)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 24632:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 15151 - 95858:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS29565: Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (723..1138): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LDQAFDASVWEMTVVVAGEVNAYGPTEVTVCATM
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

F5972_RS29780: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (50..453): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: PHLSWGKHAWSACAVAAGERDGYGQTETTAQIGT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS29845: X

AMP-binding (89..512): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MPPLHLVARRLKAMVLGGAFDSYGTTETGLVMLD
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
GnfM	Regulator of nitrogen-fixation genes
MexT	Global virulence regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000014.1 - Region 2 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 158,029 - 183,229 nt. (total: 25,201 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

F5972_RS30185: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

F5972_RS30190: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.43	ribosomal	planosporicin	Planomonospora alba
BGC0001727.3	0.43	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000506.4	0.41	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.40	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002294.3	0.35	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0002509.3	0.34	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0000213.4	0.26	PKS	colabomycin E	Streptomyces aureus
BGC0000360.5	0.24	NRPS	GE81112	Streptomyces sp. L-49973
BGC0000907.5	0.24	other (other)	lincomycin	Streptomyces lincolnensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.43	ribosomal	planosporicin	Planomonospora alba
BGC0001727.3	0.43	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000506.4	0.41	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.40	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002294.3	0.35	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0002509.3	0.34	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0000213.4	0.26	PKS	colabomycin E	Streptomyces aureus
BGC0000360.5	0.24	NRPS	GE81112	Streptomyces sp. L-49973
BGC0000907.5	0.24	other (other)	lincomycin	Streptomyces lincolnensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for F5972_RS30190

F5972_RS30195

Leader:

MSTQTITRSAEPRQHASDEADDPFQLDITFIENTPATE

Core:

TVLLCSTGDNCGSSCPSACTTS

Core with dehydrations:

DhbVLLCysDhaDhbGDNCysGDhaDhaCysPDhaACysDhbDhbDha

antiSMASH-DB 4.0 matches for F5972_RS30195

100.0%

NZ_KI866523.1and 2 others:

NZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptideNZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptide

Lanthipeptides: MPTA5024_RS18085_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVLLCSTGDNCGSSCPSACTTS

1

TVLLCSTGDNCGSSCPSACTTS

22

95.5%

NZ_CP029042.1:

Lanthipeptides: DDE74_RS17120_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGDNCGSSCPSACTTS

1

TVLMCSTGDNCGSSCPSACTTS

22

95.5%

NZ_CP036455.1:

Lanthipeptides: EKD16_RS21370_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

T LLCSTGDNCGSSCPSACTTS

1

TELLCSTGDNCGSSCPSACTTS

22

with 173 more sequence matches

Only the first 10 are displayed here.

90.9%

NZ_BBYG01000011.1and 23 others:

NZ_BBZE01000004.1 Lanthipeptides: AOQ56_RS00075_lanthipeptideNZ_BMUG01000008.1 Lanthipeptides: IE264_RS24565_lanthipeptideNZ_CP013219.1 Lanthipeptides: SHL15_RS30830_lanthipeptideNZ_CP021978.1 Lanthipeptides: CEB94_RS33350_lanthipeptideNZ_CP029342.1 Lanthipeptides: B9S64_RS02555_lanthipeptideNZ_CP030073.1 Lanthipeptides: DN051_RS07240_lanthipeptideNZ_CP041610.2 Lanthipeptides: FNV62_RS40125_lanthipeptideNZ_CP041613.2 Lanthipeptides: FNV68_RS42745_lanthipeptideNZ_CP051006.1 Lanthipeptides: HEP81_RS07390_lanthipeptideNZ_CP074102.1 Lanthipeptides: SMIR_RS04425_lanthipeptideNZ_CP101397.1 Lanthipeptides: NLU04_RS20150_lanthipeptideNZ_CP113910.1 Lanthipeptides: HUV60_RS04250_lanthipeptideNZ_FKJI03000004.1 Lanthipeptides: A7P67_RS09720_lanthipeptideNZ_JACHCX010000047.1 Lanthipeptides: HDC93_RS37275_lanthipeptideNZ_KQ948477.1 Lanthipeptides: AQJ27_RS43755_lanthipeptideNZ_KQ948550.1 Lanthipeptides: AQJ30_RS07460_lanthipeptideNZ_LIVY01000006.1 Lanthipeptides: AMK30_RS21645_lanthipeptideNZ_LMDL01000002.1 Lanthipeptides: ASD08_RS03885_lanthipeptideNZ_RAII01000001.1 Lanthipeptides: EBH12_RS06225_lanthipeptideNZ_SHKS01000004.1 Lanthipeptides: EV567_RS16240_lanthipeptideNZ_SHKU01000001.1 Lanthipeptides: EV284_RS02155_lanthipeptideNZ_SZNQ01000001.1 Lanthipeptides: E4U91_RS28815_lanthipeptideNZ_VIUV01000003.1 Lanthipeptides: FB570_RS08885_lanthipeptide

Lanthipeptides: AOQ37_RS11080_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGD CGSSCPSACTTS

1

TVLMCSTGDTCGSSCPSACTTS

22

90.9%

NZ_CP045178.1:

Lanthipeptides: GBW32_RS14380_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

T+L+CSTGDNCGSSCPSACTTS

1

TMLMCSTGDNCGSSCPSACTTS

22

90.9%

NZ_ML123117.1:

Lanthipeptides: EES46_RS09865_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+C TGDNCGSSCPSACTTS

1

TVLMCGTGDNCGSSCPSACTTS

22

86.4%

NZ_BMVW01000003.1and 2 others:

NZ_CP011799.1 Lanthipeptides: QR97_RS02045_lanthipeptideNZ_JAEVFI010000002.1 Lanthipeptides: JJV70_RS02680_lanthipeptide

Lanthipeptides: IE242_RS12765_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGD CGSSCPSAC TS

1

TVLMCSTGDTCGSSCPSACVTS

22

86.4%

NZ_CP050976.1:

Lanthipeptides: HEP86_RS36805_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+C+TGD CGSSCPSACTTS

1

TVLMCTTGDTCGSSCPSACTTS

22

86.4%

NZ_JAHLXF010000013.1:

Lanthipeptides: KQY13_RS21225_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCGSSCPSACTTS

1

LMCSTGDNCGSSCPSACTTS

20

81.8%

NZ_BMSO01000039.1and 5 others:

NZ_BMTU01000002.1 Lanthipeptides: IE118_RS05930_lanthipeptideNZ_BMUK01000002.1 Lanthipeptides: IE276_RS05195_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS30040_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptide

Lanthipeptides: IE184_RS41715_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGD CGSSCPSACTTS

1

LMCSTGDTCGSSCPSACTTS

20

81.8%

NZ_CP070379.1:

Lanthipeptides: JVE63_RS10125_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL C+TGD CGSSCPSAC TS

1

TVLTCTTGDTCGSSCPSACVTS

22

81.8%

NZ_FOQY01000053.1:

Lanthipeptides: BM085_RS43985_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCG+SCPSACTTS

1

LMCSTGDNCGTSCPSACTTS

20

79.2%

NZ_BNBV01000037.1:

Lanthipeptides: IE286_RS24415_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL CSTGDNCGSSCPSAC+ S

1

TVLTCSTGDNCGSSCPSACSGS

22

Lanthipeptide(s) for F5972_RS30220

F5972_RS30195

Leader:

MSTQTITRSAEPRQHASDEADDPFQLDITFIENTPATE

Core:

TVLLCSTGDNCGSSCPSACTTS

Core with dehydrations:

DhbVLLCysDhaDhbGDNCysGDhaDhaCysPDhaACysDhbDhbDha

antiSMASH-DB 4.0 matches for F5972_RS30195

100.0%

NZ_KI866523.1and 2 others:

NZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptideNZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptide

Lanthipeptides: MPTA5024_RS18085_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVLLCSTGDNCGSSCPSACTTS

1

TVLLCSTGDNCGSSCPSACTTS

22

95.5%

NZ_CP029042.1:

Lanthipeptides: DDE74_RS17120_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGDNCGSSCPSACTTS

1

TVLMCSTGDNCGSSCPSACTTS

22

95.5%

NZ_CP036455.1:

Lanthipeptides: EKD16_RS21370_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

T LLCSTGDNCGSSCPSACTTS

1

TELLCSTGDNCGSSCPSACTTS

22

with 173 more sequence matches

Only the first 10 are displayed here.

90.9%

NZ_BBYG01000011.1and 23 others:

NZ_BBZE01000004.1 Lanthipeptides: AOQ56_RS00075_lanthipeptideNZ_BMUG01000008.1 Lanthipeptides: IE264_RS24565_lanthipeptideNZ_CP013219.1 Lanthipeptides: SHL15_RS30830_lanthipeptideNZ_CP021978.1 Lanthipeptides: CEB94_RS33350_lanthipeptideNZ_CP029342.1 Lanthipeptides: B9S64_RS02555_lanthipeptideNZ_CP030073.1 Lanthipeptides: DN051_RS07240_lanthipeptideNZ_CP041610.2 Lanthipeptides: FNV62_RS40125_lanthipeptideNZ_CP041613.2 Lanthipeptides: FNV68_RS42745_lanthipeptideNZ_CP051006.1 Lanthipeptides: HEP81_RS07390_lanthipeptideNZ_CP074102.1 Lanthipeptides: SMIR_RS04425_lanthipeptideNZ_CP101397.1 Lanthipeptides: NLU04_RS20150_lanthipeptideNZ_CP113910.1 Lanthipeptides: HUV60_RS04250_lanthipeptideNZ_FKJI03000004.1 Lanthipeptides: A7P67_RS09720_lanthipeptideNZ_JACHCX010000047.1 Lanthipeptides: HDC93_RS37275_lanthipeptideNZ_KQ948477.1 Lanthipeptides: AQJ27_RS43755_lanthipeptideNZ_KQ948550.1 Lanthipeptides: AQJ30_RS07460_lanthipeptideNZ_LIVY01000006.1 Lanthipeptides: AMK30_RS21645_lanthipeptideNZ_LMDL01000002.1 Lanthipeptides: ASD08_RS03885_lanthipeptideNZ_RAII01000001.1 Lanthipeptides: EBH12_RS06225_lanthipeptideNZ_SHKS01000004.1 Lanthipeptides: EV567_RS16240_lanthipeptideNZ_SHKU01000001.1 Lanthipeptides: EV284_RS02155_lanthipeptideNZ_SZNQ01000001.1 Lanthipeptides: E4U91_RS28815_lanthipeptideNZ_VIUV01000003.1 Lanthipeptides: FB570_RS08885_lanthipeptide

Lanthipeptides: AOQ37_RS11080_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGD CGSSCPSACTTS

1

TVLMCSTGDTCGSSCPSACTTS

22

90.9%

NZ_CP045178.1:

Lanthipeptides: GBW32_RS14380_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

T+L+CSTGDNCGSSCPSACTTS

1

TMLMCSTGDNCGSSCPSACTTS

22

90.9%

NZ_ML123117.1:

Lanthipeptides: EES46_RS09865_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+C TGDNCGSSCPSACTTS

1

TVLMCGTGDNCGSSCPSACTTS

22

86.4%

NZ_BMVW01000003.1and 2 others:

NZ_CP011799.1 Lanthipeptides: QR97_RS02045_lanthipeptideNZ_JAEVFI010000002.1 Lanthipeptides: JJV70_RS02680_lanthipeptide

Lanthipeptides: IE242_RS12765_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+CSTGD CGSSCPSAC TS

1

TVLMCSTGDTCGSSCPSACVTS

22

86.4%

NZ_CP050976.1:

Lanthipeptides: HEP86_RS36805_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL+C+TGD CGSSCPSACTTS

1

TVLMCTTGDTCGSSCPSACTTS

22

86.4%

NZ_JAHLXF010000013.1:

Lanthipeptides: KQY13_RS21225_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCGSSCPSACTTS

1

LMCSTGDNCGSSCPSACTTS

20

81.8%

NZ_BMSO01000039.1and 5 others:

NZ_BMTU01000002.1 Lanthipeptides: IE118_RS05930_lanthipeptideNZ_BMUK01000002.1 Lanthipeptides: IE276_RS05195_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS30040_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptide

Lanthipeptides: IE184_RS41715_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGD CGSSCPSACTTS

1

LMCSTGDTCGSSCPSACTTS

20

81.8%

NZ_CP070379.1:

Lanthipeptides: JVE63_RS10125_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL C+TGD CGSSCPSAC TS

1

TVLTCTTGDTCGSSCPSACVTS

22

81.8%

NZ_FOQY01000053.1:

Lanthipeptides: BM085_RS43985_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCG+SCPSACTTS

1

LMCSTGDNCGTSCPSACTTS

20

79.2%

NZ_BNBV01000037.1:

Lanthipeptides: IE286_RS24415_lanthipeptide

1

TVLLCSTGDNCGSSCPSACTTS

22

TVL CSTGDNCGSSCPSAC+ S

1

TVLTCSTGDNCGSSCPSACSGS

22

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.97 out of 30.22

Confidence: medium

Additional weak hits (3):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.84 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.75 out of 31.73

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

F5972_RS30195 - Class I

Cleavage pHMM score: -5.30

RODEO score: 23

Monoisotopic mass: 1943.8 Da

Molecular weight: 1945.1 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 1963.2 Da N = 1; 1981.2 Da N = 2; 1999.2 Da N = 3; 2017.2 Da N = 4; 2035.2 Da N = 5

F5972_RS30195 - Class I

Cleavage pHMM score: -5.30

RODEO score: 23

Monoisotopic mass: 1943.8 Da

Molecular weight: 1945.1 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 1963.2 Da N = 1; 1981.2 Da N = 2; 1999.2 Da N = 3; 2017.2 Da N = 4; 2035.2 Da N = 5

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 156 - 240; Score: 63.0

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
ClgR	Caseinolytic protease gene regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000015.1 - Region 1 - terpene

Download region GenBank file

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Location: 90,304 - 111,236 nt. (total: 20,933 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS30690: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

F5972_RS30720: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Ligases: synthase

F5972_RS30695: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.53	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.53	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.51	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.51	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.48	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.47	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000645.3	0.46	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000648.3	0.43	terpene	carotenoid	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.53	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.53	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.51	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.51	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.48	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.47	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000645.3	0.46	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000648.3	0.43	terpene	carotenoid	Myxococcus xanthus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene		squalene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

F5972_RS30715: PT_phytoene_like

Domain 1 (16 - 305): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → PSPP; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (12):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.61 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.04 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.15 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.50 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.34 out of 20.97

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.65 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.67 out of 27.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.34 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.58 out of 35.63

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.08 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.08 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_VYTZ01000016.1 - Region 1 - terpene

Download region GenBank file

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Location: 84,536 - 107,068 nt. (total: 22,533 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

F5972_RS31540: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS31555: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Translocases: transporter

F5972_RS31590: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002043.3	0.54	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.51	terpene	raimonol	Streptomyces anulatus
BGC0001197.5	0.44	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000286.3	0.42	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001483.4	0.42	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001840.2	0.39	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0001315.3	0.37	terpene	cucurbitacin C	Cucumis sativus
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002043.3	0.54	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.51	terpene	raimonol	Streptomyces anulatus
BGC0001197.5	0.44	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000286.3	0.42	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001483.4	0.42	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001840.2	0.39	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0001315.3	0.37	terpene	cucurbitacin C	Cucumis sativus
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
diterpene			C20	C6-C11 + C10-C15	PP

Genes with terpene domains:

F5972_RS31565: T2TS

Domain 1 (3 - 490): Type 2 terpene synthase

Subtype: Diterpene synthase

Reactions:: GGPP → diterpene_C6-C11/C10-C15_PP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.66 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.06 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.79 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.24 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.95 out of 29.65

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.04 out of 30.98

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.87 out of 36.53

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_VYTZ01000016.1 - Region 2 - NRPS,T3PKS

Download region GenBank file

Download region SVG

Location: 119,457 - 194,694 nt. (total: 75,238 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: halogenase methyltransferase

F5972_RS31685: methyltransferase

smcogs: SMCOG1089: methyltransferase

F5972_RS31695: methyltransferase

smcogs: SMCOG1089: methyltransferase

F5972_RS31795: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000040Halogenation (identity: 69.10%)
smcogs: SMCOG1119: halogenase

Hydrolases: hydrolase

F5972_RS31800: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: hydratase

F5972_RS31790: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

F5972_RS31730: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS31780: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

F5972_RS31825: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.94	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.91	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.59	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.51	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002071.2	0.51	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135.2	0.44	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.42	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.41	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.76	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 22.47 out of 25.31

Confidence: strong

Additional weak hits (15):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.31 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.12 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.86 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.14 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.56 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.43 out of 20.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.05 out of 27.55

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.74 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.99 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.38 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.33 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.35 out of 20.97

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.18 out of 27.18

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.00 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 119456 - 194694:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Dhpg - D-Dhpg - Phe - D-Dhpg) + (Tyr - Pro - Dhpg - X - Tyr - Pro - Dhpg - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 119456 - 160566:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Dhpg - D-Dhpg - Phe - D-Dhpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 130142 - 194694:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Dhpg - D-Dhpg - Phe - D-Dhpg) + (Tyr - Pro - Dhpg - X - Tyr - Pro - Dhpg - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS31730: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (6..375): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: NALH---LILGGFGVSGSALNRYGMTETLVTLSG
Nearest Stachelhaus code(s):: Leu DALFVGAVAK (35% 8Å match); Val DALFVGLVAK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

F5972_RS31825: Dhpg - Dhpg - Phe - Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (118..553): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1759..2174): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3388..3789): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LVHSFDASVWEMTVVVAGEVNAYGPTETTVCATM
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4459..4852): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

F5972_RS31830: Tyr - Pro - Dhpg - X - Tyr - Pro - Dhpg - X

Search NORINE for peptide: strict or relaxed

AMP-binding (464..863): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDAAVSEMTVVVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1529..1925): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LAHTFDVAFAETMTSVAGEVNAYGPTEATVIATM
Nearest Stachelhaus code(s):: Trp DVAMVGAVCK (68% 8Å match); Ala DVWIYAAVIK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2590..3003): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3692..4090): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVVGAEWVAYGPTEASVIATS
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (82% 8Å match); Hpg DALSIGAVIK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4786..5189): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDAAVSEMTVVVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5854..6250): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LAHTFDVAFAETMMSVAGEVNAYGPTEATVIATM
Nearest Stachelhaus code(s):: Trp DVAMVGAVCK (68% 8Å match); Ala DVWIYAAVIK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (6914..7326): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8036..8429): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVVGAEWVAYGPTEASVIATT
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (82% 8Å match); Hpg DALSIGAVIK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor

NZ_VYTZ01000017.1 - Region 1 - phenazine

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Location: 59,463 - 79,951 nt. (total: 20,489 nt) Show pHMM detection rules used

phenazine: phzB

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

F5972_RS32175: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

F5972_RS32180: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.66	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.60	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001221.5	0.50	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000934.4	0.48	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001868.4	0.46	other (phenazine)	iodinin	Xenorhabdus szentirmaii DSM 16338
BGC0001080.5	0.45	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001302.5	0.45	other (other)	lomofungin	Streptomyces lomondensis
BGC0000343.5	0.44	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002557.2	0.43	terpene	penifulvin A	Penicillium griseofulvum
BGC0002439.3	0.41	other (other)	diastaphenazine, izumiphenazine C	Streptomyces diastaticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.66	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.60	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001221.5	0.50	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000934.4	0.48	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001868.4	0.46	other (phenazine)	iodinin	Xenorhabdus szentirmaii DSM 16338
BGC0001080.5	0.45	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001302.5	0.45	other (other)	lomofungin	Streptomyces lomondensis
BGC0000343.5	0.44	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002557.2	0.43	terpene	penifulvin A	Penicillium griseofulvum
BGC0002439.3	0.41	other (other)	diastaphenazine, izumiphenazine C	Streptomyces diastaticus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.12 out of 23.79

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.84 out of 30.12

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.90 out of 30.12

Confidence: medium

Additional weak hits (10):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.56 out of 21.55

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.76 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.60 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.95 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.93 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.08 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 22.78 out of 36.54

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.09 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator

NZ_VYTZ01000018.1 - Region 1 - lanthipeptide-class-iv

Download region GenBank file

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Location: 6,440 - 29,223 nt. (total: 22,784 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS32745: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.60	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002337.2	0.57	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000513.3	0.56	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.56	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000496.5	0.56	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001226.4	0.54	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000553.3	0.46	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.60	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002337.2	0.57	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000513.3	0.56	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.56	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000496.5	0.56	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001226.4	0.54	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000553.3	0.46	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.36 out of 25.31

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.42 out of 19.52

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (12):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.17 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.18 out of 36.54

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.81 out of 26.85

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.13 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.02 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.32 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.95 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.81 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.35 out of 24.78

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.45 out of 29.25

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.23 out of 26.85

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000002.1 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 515,290 - 538,166 nt. (total: 22,877 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

F5972_RS06295: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

F5972_RS06345: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001576.3	0.46	ribosomal	divamide A	Prochloron didemni
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000503.5	0.45	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001576.3	0.46	ribosomal	divamide A	Prochloron didemni
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000503.5	0.45	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for F5972_RS06345

F5972_RS06335

Leader:

MPVTLDRSEEIVSAWRSGADVEGWDSPAG

Core:

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

Core with dehydrations:

PLFIGEYAEDhaEIDhbLDhbALDDhbRRPDhaDhaCysDhbGANDhbIPCysCys

antiSMASH-DB 4.0 matches for F5972_RS06335

70.6%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26720_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05225_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06340_lanthipeptide

Lanthipeptides: HHX37_RS08025_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCT

26

PLFIGEYAESEITLTALDTR SSCT

1

PLFIGEYAESEITLTALDTRLASSCT

26

69.4%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26715_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05230_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06335_lanthipeptide

Lanthipeptides: HHX37_RS08020_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

34

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

16

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

49

48.9%

NZ_BMQJ01000023.1:

Lanthipeptides: IEZ10_RS33030_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCT

26

PLFIG+YAESEITLTALDTR SSCT

16

PLFIGDYAESEITLTALDTRLASSCT

41

with 39 more sequence matches

Only the first 10 are displayed here.

48.6%

NZ_JANZKX010000002.1:

Lanthipeptides: O9Y01_RS08280_lanthipeptide

1

PLFI-GEYAESEITL-TALDTRRP-SSCTGANTIPCC

34

PLF G Y+E+EI + +TR+ SSC+G+ TI CC

1

PLFSSGVYSEAEIAGDVSRETRQGCSSCSGSRTIMCC

37

47.2%

NZ_JALPRT010000019.1and 1 other:

NZ_KB895919.1 Lanthipeptides: C570_RS1000000130195_lanthipeptide

Lanthipeptides: M0M45_RS13255_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRRPSSCTGANTIPCC

34

PLF G Y EIT T + SSCTG+ I CC

1

PLFTGGRYVVQEITSTWGGCSTVCSSCTGSVPIQCC

36

44.4%

NZ_AZWQ01000024.1:

Lanthipeptides: B116_RS0113780_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRRPSSCTGANTIPCC

34

PLF G +Y EIT T + SSCTG+ + CC

1

PLFTGGKYVVQEITSTWGGCSTVCSSCTGSQRVSCC

36

41.5%

NZ_CP079946.1:

Lanthipeptides: KYK13_RS20175_lanthipeptide

1

PLFIG----EYAESEITLTALDTRRPSSCTGANTIPCC

34

P+F+G E A +E L A DT SSCT + T CC

6

PVFVGGTSTEKALTESNLVA-DTGC-SSCTASTTAFCC

41

40.0%

NZ_FWXV01000001.1:

Lanthipeptides: B9C43_RS03005_lanthipeptide

1

PLFI-GEYAESEITLTALDTRRPSSCTGANTIPCC

34

P+F GE+AE +I R SSCT + + CC

1

PIFASGEFAEGDIVACNPPGSRCSSCTASRPVFCC

35

40.0%

NZ_JANYMP010000013.1:

Lanthipeptides: NZH93_RS25860_lanthipeptide

1

PLFI-GEYAESEITLTALDTRRPSSCTGANTIPCC

34

PLF GE+AE +I R S+CT + + CC

1

PLFASGEFAEGDIVACNPPGSRCSACTASRPVVCC

35

39.5%

NZ_CP064030.1:

Lanthipeptides: ISN74_RS08625_lanthipeptide

1

PLFI-GEYAESEI-------TLTALDTRR-PSSCTGANTIPCC

34

PLF+ G++AES+I TL A+ + R S+C+G+ CC

1

PLFVSGQFAESDIVSSTNVLTLGAVCSGRCGSACSGSAHAECC

43

37.8%

NZ_AZVD01000036.1and 1 other:

NZ_KB913036.1 Lanthipeptides: A39M_RS0105590_lanthipeptide

Lanthipeptides: K389_RS0119060_lanthipeptide

1

PLFIG-EYAESEITLTAL-DTRRPSSCTGAN

29

PLF G Y EIT TA+ + S+CTG+

1

PLFTGGRYVVQEITATAVANWTYCSACTGSG

31

37.5%

NZ_CP072597.1:

Lanthipeptides: J5226_RS01600_lanthipeptide

1

PLFI-GEYAESEITL-TALDTRRPSSCTGANTIPCC

34

PLFI GE+AE++I + +R S+CTG TI CC

13

PLFISGEFAEADIAGDNEIISRNTSACTGCGTIQCC

48

37.5%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

1

PLFIG-EYAESEITLTALDTRR----PSSCTGANTIPCC

34

PLF G ++AE+EI A ++CTG++T CC

2

PLFTGGQFAEAEIISEASSLTTFGGCGTACTGSHTRYCC

40

F5972_RS06340

Leader:

MPLTLDRSEEIVSAWRSGADVEGWDSPAG

Core:

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

Core with dehydrations:

PLFIGEYAEDhaEIDhbLDhbALDDhbRLADhaDhaCysDhbCysDhaRFCysCys

antiSMASH-DB 4.0 matches for F5972_RS06340

100.0%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26720_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05225_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06340_lanthipeptide

Lanthipeptides: HHX37_RS08025_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

66.0%

NZ_BMQJ01000023.1:

Lanthipeptides: IEZ10_RS33030_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

PLFIG+YAESEITLTALDTRLASSCTCSRFCC

16

PLFIGDYAESEITLTALDTRLASSCTCSRFCC

47

49.0%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26715_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05230_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06335_lanthipeptide

Lanthipeptides: HHX37_RS08020_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCT

26

PLFIGEYAESEITLTALDTR SSCT

16

PLFIGEYAESEITLTALDTRRPSSCT

41

with 26 more sequence matches

Only the first 10 are displayed here.

45.7%

NZ_FWXV01000001.1:

Lanthipeptides: B9C43_RS03005_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCSR--FCC

32

P+F GE+AE +I SSCT SR FCC

1

PIFASGEFAEGDIVACNPPGSRCSSCTASRPVFCC

35

40.5%

NZ_CP048405.1:

Lanthipeptides: GXW83_RS14070_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCS----RFCC

32

PLF G YAESE+T + + CS R CC

1

PLFTSGAYAESELTASGAVRTIQCGTACSYSAGRECC

37

40.0%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

1

PLFIG-EYAESEI-----TLTALDTRLASSCTCS--RFCC

32

PLF G ++AE+EI +LT ++CT S R+CC

2

PLFTGGQFAEAEIISEASSLTTFG-GCGTACTGSHTRYCC

40

38.9%

NZ_AZWQ01000024.1:

Lanthipeptides: B116_RS0113780_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRLASSCTCSR

29

PLF G +Y EIT T + + SSCT S+

1

PLFTGGKYVVQEITSTWGGCSTVCSSCTGSQ

31

37.8%

NZ_AZVD01000036.1and 1 other:

NZ_KB913036.1 Lanthipeptides: A39M_RS0105590_lanthipeptide

Lanthipeptides: K389_RS0119060_lanthipeptide

1

PLFIG-EYAESEITLTAL-DTRLASSCTCS

28

PLF G Y EIT TA+ + S+CT S

1

PLFTGGRYVVQEITATAVANWTYCSACTGS

30

37.1%

NZ_JANYMP010000013.1:

Lanthipeptides: NZH93_RS25860_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCSR

29

PLF GE+AE +I S+CT SR

1

PLFASGEFAEGDIVACNPPGSRCSACTASR

30

37.0%

NZ_QFWW01000008.1:

Lanthipeptides: DI276_RS14925_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCS--RFCC

32

PLF GEYAE E+T+T + + CT S R CC

12

PLFPSGEYAEHELTMTGHSGKCGTVCTYSQGRQCC

46

36.1%

NZ_JALPRT010000019.1and 1 other:

NZ_KB895919.1 Lanthipeptides: C570_RS1000000130195_lanthipeptide

Lanthipeptides: M0M45_RS13255_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRLASSCT

26

PLF G Y EIT T + + SSCT

1

PLFTGGRYVVQEITSTWGGCSTVCSSCT

28

35.3%

NZ_CP034550.1:

Lanthipeptides: EKG83_RS30035_lanthipeptide

5

GEYAESEITLTALDTRLASSCTCSR--FCC

32

GE+AE +I SSCT S +CC

5

GEFAEGDIVACNPPGSRCSSCTASAHVYCC

34

34.7%

NZ_QOIL01000017.1:

Lanthipeptides: DQ384_RS40545_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTC-SRFCC

32

P IGEY E EIT++ ++T+ SSCT FCC

17

PPLIGEYTEEEITMSLIETKRPSSCTAGGGFCC

49

allorf_519450_519632

Leader:

MATGRRRGRASRRDAGAGPRAWSGPGTRRDETGA

Core:

RCFRRHRVTLSIELGDSGRGKSISWG

Core with dehydrations:

RCysFRRHRVDhbLDhaIELGDDhaGRGKDhaIDhaWG

antiSMASH-DB 4.0 matches for allorf_519450_519632

100.0%

NZ_VYTZ01000002.1:

Lanthipeptides: allorf_519450_519632_lanthipeptide

1

RCFRRHRVTLSIELGDSGRGKSISWG

26

RCFRRHRVTLSIELGDSGRGKSISWG

1

RCFRRHRVTLSIELGDSGRGKSISWG

26

73.1%

NZ_JABBWY010000002.1and 1 other:

NZ_JABBWZ010000011.1 Lanthipeptides: allorf_012175_012357_lanthipeptide

Lanthipeptides: allorf_683196_683378_lanthipeptide

1

RCFRRHRVTLSIELGDSGRGKSISW

25

RCF HR +L +ELGDSGRGKSISW

1

RCFPPHRASLLMELGDSGRGKSISW

25

73.1%

NZ_VANP01000002.1:

Lanthipeptides: allorf_020225_020401_lanthipeptide

1

RCFRRHRVTLSIELGDSGRGKSIS

24

RCF HR TL +ELGDSGRGKSIS

1

RCFPPHRATLLMELGDSGRGKSIS

24

with 3 more sequence matches

30.8%

NZ_CP007153.1:

Lanthipeptides: allorf_02389419_02389625_lanthipeptide

9

TLSIELGDSGRGKSISWG

26

TLSIE G + +WG

5

TLSIEAPCPGWPSTFTWG

22

21.2%

NZ_KL997439.1and 1 other:

NZ_KL997439.1 Lanthipeptides: IQ62_RS47105_lanthipeptide

Lanthipeptides: IQ62_RS47105_lanthipeptide

9

TLSIELGDSG

18

TLS +LGD G

10

TLSAQLGDKG

19

20.6%

NZ_SLWA01000002.1:

Lanthipeptides: EV142_RS05900_lanthipeptide

1

RCFRRHRVTLSIELGDSGRG

20

+C+ VTLS D G+

15

KCWNEKTVTLSCSSCDFGQA

34

MIBiG 4.0 matches for allorf_519450_519632

19.2%

BGC0002150.2 (MBO0654592.1):

Lasso peptide: triculamin

13

ELGDSGRGKSI

23

+ GD RGK +

5

KPGDGIRGKGV

15

allorf_524647_524781

Leader:

MEGGRSRSRVPDIPSASRQAPSQSTAAFA

Core:

ISAECRQVYVSLAMY

Core with dehydrations:

IDhaAECysRQVYVDhaLAMY

antiSMASH-DB 4.0 matches for allorf_524647_524781

100.0%

NZ_VYTZ01000002.1:

Lanthipeptides: allorf_524647_524781_lanthipeptide

1

ISAECRQVYVSLAMY

15

ISAECRQVYVSLAMY

1

ISAECRQVYVSLAMY

15

32.5%

NZ_JABBWY010000002.1and 1 other:

NZ_JABBWZ010000011.1 Lanthipeptides: allorf_016510_016680_lanthipeptide

Lanthipeptides: allorf_687494_687664_lanthipeptide

1

ISAECRQVYVSLAMY

15

ISAECRQVY+S AMY

26

ISAECRQVYISPAMY

40

25.0%

NC_012470.1and 50 others:

NZ_ALSD01000046.1 Lanthipeptides: SAG0161_RS1000000124770_lanthipeptideNZ_CAAHGW010000010.1 Lanthipeptides: E9435_RS08895_lanthipeptideNZ_CAAHHE010000002.1 Lanthipeptides: E9499_RS02930_lanthipeptideNZ_CAAHHF010000009.1 Lanthipeptides: E9478_RS08675_lanthipeptideNZ_CAAHHN010000003.1 Lanthipeptides: E9502_RS05705_lanthipeptideNZ_CAAHHU010000009.1 Lanthipeptides: E9510_RS08735_lanthipeptideNZ_CAAHHW010000012.1 Lanthipeptides: E9525_RS08985_lanthipeptideNZ_CAAHIB010000001.1 Lanthipeptides: E9539_RS01075_lanthipeptideNZ_CAAHIV010000024.1 Lanthipeptides: E9612_RS09255_lanthipeptideNZ_CAAHKK010000019.1 Lanthipeptides: E9649_RS08770_lanthipeptideNZ_CAAHKP010000014.1 Lanthipeptides: E9652_RS08920_lanthipeptideNZ_CAAHKQ010000003.1 Lanthipeptides: E9664_RS02160_lanthipeptideNZ_CAAHKY010000014.1 Lanthipeptides: E9668_RS09650_lanthipeptideNZ_CAAHLP010000002.1 Lanthipeptides: E9684_RS03445_lanthipeptideNZ_CAAHQE010000012.1 Lanthipeptides: E9931_RS08815_lanthipeptideNZ_CAAHRY010000002.1 Lanthipeptides: E9986_RS03175_lanthipeptideNZ_CAAIES010000002.1 Lanthipeptides: FAH15_RS02475_lanthipeptideNZ_CAAIIA010000012.1 Lanthipeptides: FAH55_RS08475_lanthipeptideNZ_CAAIJM010000015.1 Lanthipeptides: FAI23_RS09585_lanthipeptideNZ_CAAINV010000013.1 Lanthipeptides: FAI86_RS09025_lanthipeptideNZ_CAAIRS010000012.1 Lanthipeptides: FAK24_RS08485_lanthipeptideNZ_CAAJFI010000013.1 Lanthipeptides: FAP60_RS08495_lanthipeptideNZ_CABY01000005.1 Lanthipeptides: SZBHS5_RS10120_lanthipeptideNZ_CAJHJS010000009.1 Lanthipeptides: JMX87_RS06795_lanthipeptideNZ_CM001076.1 Lanthipeptides: SDD27957_RS10995_lanthipeptideNZ_CP018188.1 Lanthipeptides: BSR19_RS11185_lanthipeptideNZ_CP029398.1 Lanthipeptides: DF184_RS05190_lanthipeptideNZ_CP035426.1 Lanthipeptides: ETT73_RS02600_lanthipeptideNZ_CP035428.1 Lanthipeptides: ETT71_RS02820_lanthipeptideNZ_CP035437.1 Lanthipeptides: ETT62_RS06670_lanthipeptideNZ_CP035443.1 Lanthipeptides: ETT56_RS07260_lanthipeptideNZ_CP060646.1 Lanthipeptides: H7789_RS06840_lanthipeptideNZ_CP066093.1 Lanthipeptides: I6I02_RS07305_lanthipeptideNZ_CP078012.1 Lanthipeptides: KV238_RS09825_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS1000000113590_lanthipeptideNZ_JAFLMI010000009.1 Lanthipeptides: J0638_RS05340_lanthipeptideNZ_JALDUD010000010.1 Lanthipeptides: M1G10_RS09695_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09305_lanthipeptideNZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09305_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06590_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06590_lanthipeptideNZ_KV832031.1 Lanthipeptides: HMPREF2637_RS10860_lanthipeptideNZ_LR738724.1 Lanthipeptides: GPW69_RS05070_lanthipeptideNZ_LR793258.1 Lanthipeptides: HV369_RS03150_lanthipeptideNZ_LS483425.1 Lanthipeptides: DQN36_RS06745_lanthipeptideNZ_LT545678.1 Lanthipeptides: BJR96_RS11590_lanthipeptideNZ_NWMU01000024.1 Lanthipeptides: CO197_RS09155_lanthipeptideNZ_PKIA01000004.1 Lanthipeptides: CYK22_RS04325_lanthipeptideNZ_VMMF01000004.1 Lanthipeptides: HT717_RS01580_lanthipeptideNZ_VZNS01000007.1 Lanthipeptides: F6P79_RS05570_lanthipeptide

Lanthipeptides: SZO_RS10260_lanthipeptide

1

ISAECR

6

ISAECR

8

ISAECR

13

allorf_533472_533681

Leader:

MPGNGAGADRLTFTVDEPAG

Core:

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLLGGKGETVV

Core with dehydrations:

RPRQGLAPCysRGRDhaGLHRAGWDhaVDhbPDhbRGDPRDDhaADhbENRPPLLGGKGEDhbVV

antiSMASH-DB 4.0 matches for allorf_533472_533681

100.0%

NZ_VYTZ01000002.1:

Lanthipeptides: allorf_533472_533681_lanthipeptide

1

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLLGGKGETVV

49

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLLGGKGETVV

1

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLLGGKGETVV

49

95.9%

NZ_JABBWY010000002.1:

Lanthipeptides: allorf_693655_693864_lanthipeptide

1

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLLGGKGETVV

49

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPL GKGETVV

1

RPRQGLAPCRGRSGLHRAGWSVTPTRGDPRDSATENRPPLPRGKGETVV

49

18.0%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: allorf_141930_142139_lanthipeptide

Lanthipeptides: allorf_141930_142139_lanthipeptide

6

LAPCRGRSGLHRAGWSVT

23

+APCR R L A W V

25

IAPCRWRRDLRYARWWVA

42

with 2 more sequence matches

16.3%

NZ_JAKRKC020000002.1:

Lanthipeptides: allorf_0822210_0822440_lanthipeptide

2

PRQGLAPCRGRS

13

PR G PCR RS

17

PRDGTRPCRDRS

28

10.2%

NZ_CP066762.1:

Lanthipeptides: allorf_2733265_2733486_lanthipeptide

19

GWSVTPTRGD

28

G+S+ PTR D

8

GYSICPTRHD

17

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.25 out of 29.72

Confidence: medium

Additional weak hits (10):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.25 out of 26.85

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.32 out of 26.33

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.47 out of 27.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.07 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.08 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.08 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.26 out of 18.57

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.50 out of 30.22

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.92 out of 25.75

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

F5972_RS06335 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 3484.7 Da

Molecular weight: 3486.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3504.9 Da N = 1; 3523.0 Da N = 2; 3541.0 Da N = 3; 3559.0 Da N = 4; 3577.0 Da N = 5

F5972_RS06340 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 3355.6 Da

Molecular weight: 3357.8 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3375.8 Da N = 1; 3393.9 Da N = 2; 3411.9 Da N = 3; 3429.9 Da N = 4

allorf_519450_519632 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 2882.6 Da

Molecular weight: 2884.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2902.3 Da N = 1; 2920.3 Da N = 2; 2938.3 Da N = 3; 2956.3 Da N = 4

allorf_524647_524781 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 1695.8 Da

Molecular weight: 1697.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1715.0 Da N = 1

allorf_533472_533681 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 5091.7 Da

Molecular weight: 5094.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5112.7 Da N = 1; 5130.7 Da N = 2; 5148.7 Da N = 3; 5166.7 Da N = 4; 5184.8 Da N = 5; 5202.8 Da N = 6

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C

NZ_VYTZ01000002.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 565,166 - 586,134 nt. (total: 20,969 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

F5972_RS06575: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Hydrolases: hydrolase

F5972_RS06605: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase

F5972_RS06565: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001746.3	0.76	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0001201.3	0.51	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001387.5	0.41	other (other)	nucleocidin	Streptomyces calvus
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002318.2	0.39	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.36	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002509.3	0.35	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001746.3	0.76	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0001201.3	0.51	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001387.5	0.41	other (other)	nucleocidin	Streptomyces calvus
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002318.2	0.39	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.36	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002509.3	0.35	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

F5972_RS06565: T1TS

Domain 1 (11 - 317): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.67 out of 25.31

Confidence: medium

Additional weak hits (4):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.42 out of 20.97

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 19.96 out of 27.18

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.82 out of 25.88

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor

NZ_VYTZ01000020.1 - Region 1 - redox-cofactor

Download region GenBank file

Download region SVG

Location: 34,034 - 56,882 nt. (total: 22,849 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS34045: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: transferase

F5972_RS34060: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002116.3	0.46	ribosomal	darobactin A	Photorhabdus khanii HGB 1456
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002493.3	0.41	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0001221.5	0.40	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000527.5	0.39	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002470.2	0.38	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0002284.3	0.37	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002116.3	0.46	ribosomal	darobactin A	Photorhabdus khanii HGB 1456
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002493.3	0.41	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0001221.5	0.40	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000527.5	0.39	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002470.2	0.38	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0002284.3	0.37	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (8):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.11 out of 26.33

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.88 out of 25.88

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.10 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.13 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.73 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.88 out of 30.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.68 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.95 out of 28.19

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Mycofactocin_RRE; Location: 5 - 85; Score: 93.3

TFBS definitions

Regulator	Description

NZ_VYTZ01000020.1 - Region 2 - T1PKS

Download region GenBank file

Download region SVG

Location: 58,958 - 120,244 nt. (total: 61,287 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS34135: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase

F5972_RS34175: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS34185: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: esterase hydrolase

F5972_RS34115: thioesterase

smcogs: SMCOG1004: thioesterase

F5972_RS34195: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

F5972_RS34150: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

F5972_RS34160: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS34165: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS34200: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

F5972_RS39095: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

Additional weak hits (33):

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.60 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.91 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.39 out of 30.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.10 out of 29.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.92 out of 30.97

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.79 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.20 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.53 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.30 out of 29.38

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.64 out of 30.97

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.67 out of 30.22

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.59 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.06 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.66 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.35 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.08 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.76 out of 30.25

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.56 out of 36.53

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.20 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.29 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.90 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.91 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.78 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.34 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.61 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.21 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.47 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.68 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.50 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.31 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 58957 - 120244:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (ccmal - ccmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: F5972_RS34150 + F5972_RS39095 + F5972_RS34200

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS34150: mal

PKS_AT (554..843): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.2
inactive: 88.4
Methoxymalonyl-CoA: 58.8
Methylmalonyl-CoA: 50.2
Propionyl-CoA: 23.6
Isobutyryl-CoA: 22.9
Acetyl-CoA: 22.3
Ethylmalonyl-CoA: 19.4
Benzoyl-CoA: 14.6
CHC-CoA: 10.7
fatty_acid: 10.1
trans-1,2-CPDA: 3.4
2-Methylbutyryl-CoA: 2.1
3-Methylbutyryl-CoA: -0.8

F5972_RS39095: mal - mal - mal

PKS_AT (706..1004): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 113.6
Methoxymalonyl-CoA: 65.8
Methylmalonyl-CoA: 54.2
Propionyl-CoA: 28.7
Isobutyryl-CoA: 23.4
Acetyl-CoA: 20.3
fatty_acid: 17.7
Ethylmalonyl-CoA: 14.9
Benzoyl-CoA: 11.7
CHC-CoA: 8.4
trans-1,2-CPDA: 7.3
2-Methylbutyryl-CoA: 6.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2443..2731): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.8
inactive: 106.0
Methylmalonyl-CoA: 60.0
Methoxymalonyl-CoA: 59.8
Propionyl-CoA: 36.4
Isobutyryl-CoA: 33.6
Acetyl-CoA: 25.8
2-Methylbutyryl-CoA: 13.7
CHC-CoA: 12.8
fatty_acid: 12.6
Benzoyl-CoA: 12.4
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -4.5

PKS_AT (4206..4497): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.4
inactive: 102.7
Methoxymalonyl-CoA: 62.8
Methylmalonyl-CoA: 55.8
Isobutyryl-CoA: 32.2
Propionyl-CoA: 18.1
Ethylmalonyl-CoA: 16.9
Acetyl-CoA: 15.8
Benzoyl-CoA: 12.5
fatty_acid: 8.1
CHC-CoA: 6.6
2-Methylbutyryl-CoA: 4.4
trans-1,2-CPDA: 4.2
3-Methylbutyryl-CoA: 0.0

F5972_RS34160: X

AMP-binding (2..411): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGMSFDFSLLDLNGLYSGEVNCFGSTESIAASLT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS34165: X

AMP-binding (18..410): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YLMFFDCAMFDCFSLFAGEENIYGPTELTITVTG
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (82% 8Å match); Ala DCFFFALITK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

F5972_RS34175: mmal

PKS_AT (40..278): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 13.5
CHC-CoA: 13.1
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

F5972_RS34200: mal - mal

PKS_AT (301..591): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.0
inactive: 112.7
Methoxymalonyl-CoA: 56.6
Methylmalonyl-CoA: 53.6
Isobutyryl-CoA: 33.0
Propionyl-CoA: 25.8
Acetyl-CoA: 22.7
fatty_acid: 18.4
Ethylmalonyl-CoA: 16.2
Benzoyl-CoA: 15.5
CHC-CoA: 13.1
trans-1,2-CPDA: 9.9
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.5

PKS_AT (2085..2375): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 117.1
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 54.1
Acetyl-CoA: 24.5
Propionyl-CoA: 24.1
Isobutyryl-CoA: 20.9
Ethylmalonyl-CoA: 16.5
Benzoyl-CoA: 16.5
fatty_acid: 15.2
trans-1,2-CPDA: 13.5
CHC-CoA: 10.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.3

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ToxT	Regulator of MSHA biosynthesis

NZ_VYTZ01000025.1 - Region 1 - NRPS,NRPS-like,T1PKS,terpene-precursor

Download region GenBank file

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Location: 1 - 73,029 nt. (total: 73,029 nt) Show pHMM detection rules used

Region on contig edge.

terpene-precursor: (PT_FPPS_like and not PT_HPP)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

F5972_RS35790: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

F5972_RS35850: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS35855: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

F5972_RS35860: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

F5972_RS35900: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

F5972_RS35910: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS35960: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS35975: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: acylase aminotransferase sulfhydrylase

F5972_RS35825: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

F5972_RS35830: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

F5972_RS35890: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS35945: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

F5972_RS35985: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

Hydrolases: amidase deacetylase esterase

F5972_RS35800: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

F5972_RS35865: amidase

smcogs: SMCOG1105: amidase

F5972_RS35935: thioesterase

smcogs: SMCOG1004: thioesterase

F5972_RS35940: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: lyase

F5972_RS35950: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Isomerases: isomerase

F5972_RS35920: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase synthetase

F5972_RS35780: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

F5972_RS35815: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

F5972_RS35845: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS35875: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

F5972_RS35885: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS35970: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS35990: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transport

F5972_RS35995: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.88	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000357.5	0.85	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001268.4	0.78	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

F5972_RS35815: PT_FPPS_like

Domain 1 (51 - 347): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.79 out of 21.84

Confidence: medium

Additional weak hits (19):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.21 out of 36.54

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.25 out of 30.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.67 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 21.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.25 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.60 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.75 out of 27.55

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.41 out of 33.73

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.70 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.72 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 21.84

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.13 out of 27.18

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.57 out of 30.98

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.60 out of 35.63

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.44 out of 28.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.79 out of 20.01

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.04 out of 29.65

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.26 out of 26.85

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Terpene

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 73029:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - X) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 5970 - 49327:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - mal) + (D-Tyr - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 25850 - 72801:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - X) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 36633 - 73029:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS35845: X

AMP-binding (36..433): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RTMHWAFAQLGPEALNTFDGQSWGQSETGPVTLG
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS35885: X

AMP-binding (15..416): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ATTTHTVGPMAAFWLYGGTVQTYGTTETWEIGTM
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS35890: mal

PKS_AT (5..283): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 79.5
Methoxymalonyl-CoA: 40.6
Methylmalonyl-CoA: 33.3
inactive: 20.4
Isobutyryl-CoA: 16.0
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 11.7
Ethylmalonyl-CoA: 11.6
fatty_acid: 10.7
2-Methylbutyryl-CoA: 9.0
Propionyl-CoA: 1.2
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

F5972_RS35970: Tyr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (22..411): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LEQAFDASVSEVTLIVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DASTIAAVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1546..1942): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDPRGDSLAFEIWVWTGGDVNGYGSSETAVFTTR
Nearest Stachelhaus code(s):: Ile or aIle DAFWWGGVFK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

F5972_RS35990: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (24..416): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDGCFDPSAFEGWMLGGGEVNGYGPTEDTCFTSC
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
MexT	Global virulence regulator

NZ_VYTZ01000003.1 - Region 1 - lanthipeptide-class-i

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Download region SVG

Location: 63,778 - 87,925 nt. (total: 24,148 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

F5972_RS08255: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

F5972_RS08260: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.52	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001727.3	0.39	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0001701.4	0.36	ribosomal	nisin O	Blautia obeum
BGC0000511.5	0.36	ribosomal	ericin S	Bacillus subtilis
BGC0002120.3	0.36	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000529.5	0.35	ribosomal	microbisporicin A2	Microbispora corallina
BGC0002359.3	0.30	NRPS	dudomycin A	Streptomyces albus subsp. chlorinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.52	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001727.3	0.39	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0001701.4	0.36	ribosomal	nisin O	Blautia obeum
BGC0000511.5	0.36	ribosomal	ericin S	Bacillus subtilis
BGC0002120.3	0.36	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000529.5	0.35	ribosomal	microbisporicin A2	Microbispora corallina
BGC0002359.3	0.30	NRPS	dudomycin A	Streptomyces albus subsp. chlorinus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.16 out of 25.88

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.77 out of 23.79

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 24.66 out of 29.65

Confidence: medium

Additional weak hits (9):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.37 out of 30.22

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.69 out of 20.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.61 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.03 out of 24.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.86 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.81 out of 20.01

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.93 out of 26.06

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 126 - 215; Score: 28.7

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
IolR	Regulator of the inositol catabolism
LexA variant 1	Repressor of DNA damage response
PsrA	Oleic acid responsive regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000003.1 - Region 2 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 286,940 - 308,007 nt. (total: 21,068 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

F5972_RS09380: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

F5972_RS09385: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: carbamoyltransferase phosphorylase

F5972_RS09395: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

F5972_RS09445: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Ligases: synthetase

F5972_RS09400: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001521.3	0.43	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0001529.5	0.40	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002345.2	0.40	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001521.3	0.43	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0001529.5	0.40	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002345.2	0.40	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

F5972_RS09400: PT_FPPS_like

Domain 1 (47 - 347): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.86 out of 21.84

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.27 out of 24.78

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 25.49 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.00 out of 24.78

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.29 out of 26.85

Confidence: medium

Additional weak hits (2):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.87 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.39 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
MexT	Global virulence regulator
RutR	Regulator of the pyrimidine and purine metabolism

NZ_VYTZ01000030.1 - Region 1 - T1PKS

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Location: 1 - 10,302 nt. (total: 10,302 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthase

F5972_RS36540: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.64	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.60	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.64	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.60	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 10302:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS36540: mal - mal

PKS_AT (191..481): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.2
inactive: 107.9
Methoxymalonyl-CoA: 57.3
Methylmalonyl-CoA: 53.8
Acetyl-CoA: 23.9
Propionyl-CoA: 21.9
Isobutyryl-CoA: 17.5
fatty_acid: 17.3
Benzoyl-CoA: 16.6
trans-1,2-CPDA: 13.6
Ethylmalonyl-CoA: 13.2
CHC-CoA: 10.3
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.5

PKS_AT (1996..2283): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.9
inactive: 109.8
Methoxymalonyl-CoA: 55.4
Methylmalonyl-CoA: 52.5
Isobutyryl-CoA: 25.9
Ethylmalonyl-CoA: 22.9
Acetyl-CoA: 22.8
Propionyl-CoA: 21.3
trans-1,2-CPDA: 18.5
Benzoyl-CoA: 17.1
fatty_acid: 16.1
CHC-CoA: 13.3
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.4

Link to PARAS substrate predictor

NZ_VYTZ01000033.1 - Region 1 - T1PKS

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Location: 1 - 8,331 nt. (total: 8,331 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS36635: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Ligases: synthase

F5972_RS36630: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 8331:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - Me-mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS36635: mal - mmal

PKS_AT (144..453): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 159.8
Methoxymalonyl-CoA: 61.6
inactive: 50.3
Methylmalonyl-CoA: 49.7
Propionyl-CoA: 38.3
Isobutyryl-CoA: 23.4
fatty_acid: 19.1
Ethylmalonyl-CoA: 16.9
2-Methylbutyryl-CoA: 13.2
CHC-CoA: 12.9
trans-1,2-CPDA: 8.9
Benzoyl-CoA: 8.9
3-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -3.0

PKS_AT (1947..2245): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.7
Ethylmalonyl-CoA: 206.0
Methoxymalonyl-CoA: 177.1
2-Methylbutyryl-CoA: 146.3
trans-1,2-CPDA: 128.4
Malonyl-CoA: 109.7
Propionyl-CoA: 101.7
CHC-CoA: 84.4
Isobutyryl-CoA: 83.5
Benzoyl-CoA: 66.2
Acetyl-CoA: 38.7
inactive: 37.9
3-Methylbutyryl-CoA: 36.6
fatty_acid: 30.1

Link to PARAS substrate predictor

NZ_VYTZ01000034.1 - Region 1 - NRPS

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Location: 1 - 7,501 nt. (total: 7,501 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

F5972_RS36640: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001249.5	0.62	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001249.5	0.62	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 7501:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Dhpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS36645: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (770..1196): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMAFDAAVLDVSVVLGAEWNTYGPTEASVITTA
Nearest Stachelhaus code(s):: Dhpg DALSVGTVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_VYTZ01000004.1 - Region 1 - NI-siderophore

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Location: 325,848 - 356,196 nt. (total: 30,349 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

F5972_RS12690: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

F5972_RS12715: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: protease

F5972_RS12730: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: decarboxylase

F5972_RS12685: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001572.3	0.73	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002073.3	0.70	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0001478.5	0.68	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.65	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000940.5	0.64	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002305.2	0.58	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002464.3	0.58	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001453.5	0.57	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.55	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001572.3	0.73	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002073.3	0.70	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0001478.5	0.68	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.65	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000940.5	0.64	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002305.2	0.58	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002464.3	0.58	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001453.5	0.57	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.55	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.57 out of 36.53

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.06 out of 20.01

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.57 out of 36.53

Confidence: medium

Additional weak hits (9):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.91 out of 21.55

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.03 out of 26.85

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.33 out of 20.01

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.37 out of 26.12

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.41 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.31 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.01 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.68 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.29 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
DmdR1	Iron(II)-dependent repressor

NZ_VYTZ01000004.1 - Region 2 - RRE-containing

Download region GenBank file

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Location: 631,467 - 652,591 nt. (total: 21,125 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

F5972_RS14170: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

F5972_RS14195: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Ligases: ligase

F5972_RS14155: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.56	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002276.2	0.54	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002391.2	0.52	terpene	zealexin B3, zealexin C2	Zea mays
BGC0000812.3	0.49	other (other)	huperzine A	Shiraia sp. slf14
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001137.5	0.44	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001668.4	0.41	NRPS	microperfuranone	Aspergillus nidulans FGSC A4
BGC0000595.3	0.41	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000131.5	0.40	PKS	pyrrolomycin	Streptomyces sp. UC 11065

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.56	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002276.2	0.54	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002391.2	0.52	terpene	zealexin B3, zealexin C2	Zea mays
BGC0000812.3	0.49	other (other)	huperzine A	Shiraia sp. slf14
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001137.5	0.44	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001668.4	0.41	NRPS	microperfuranone	Aspergillus nidulans FGSC A4
BGC0000595.3	0.41	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000131.5	0.40	PKS	pyrrolomycin	Streptomyces sp. UC 11065

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.07 out of 20.47

Confidence: medium

Additional weak hits (9):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.19 out of 21.55

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.59 out of 23.43

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.10 out of 27.78

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.72 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.51 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.30 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.16 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.51 out of 26.06

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.32 out of 32.93

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 1 - 85; Score: 43.7

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000005.1 - Region 1 - T1PKS

Download region GenBank file

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Location: 1 - 29,272 nt. (total: 29,272 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transferase

F5972_RS14255: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Ligases: synthase

F5972_RS14245: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

F5972_RS14250: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.64	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.64	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.02 out of 25.31

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (25):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.83 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.46 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.89 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.20 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.51 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.08 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.06 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.24 out of 29.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.61 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.03 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.00 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.59 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.73 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.21 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.93 out of 29.38

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.54 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.17 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.18 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.86 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.77 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.26 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.29 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 29272:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mxmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS14250: mxmal - mal

PKS_AT (570..798): mxmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%; Methoxymalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 89.1
Methylmalonyl-CoA: 71.1
Ethylmalonyl-CoA: 64.1
trans-1,2-CPDA: 58.9
Malonyl-CoA: 57.6
CHC-CoA: 42.5
2-Methylbutyryl-CoA: 41.3
Propionyl-CoA: 30.8
Benzoyl-CoA: 17.0
Isobutyryl-CoA: 16.2
inactive: 11.8
Acetyl-CoA: 6.3
3-Methylbutyryl-CoA: 4.9
fatty_acid: 4.1

PKS_AT (1666..1963): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.8
Methoxymalonyl-CoA: 69.3
inactive: 61.1
Methylmalonyl-CoA: 56.4
Propionyl-CoA: 46.3
Isobutyryl-CoA: 23.4
Acetyl-CoA: 20.0
Ethylmalonyl-CoA: 19.2
fatty_acid: 15.1
CHC-CoA: 14.4
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 9.0
Benzoyl-CoA: 8.6
3-Methylbutyryl-CoA: 8.3

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CatR	H2O2-responsive repressor

NZ_VYTZ01000005.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 436,503 - 461,760 nt. (total: 25,258 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS16310: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: deacetylase

F5972_RS16275: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000663.5	0.55	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000646.5	0.46	terpene	β-carotein	uncultured bacterium
BGC0001456.3	0.45	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.44	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.44	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.43	terpene	carotenoid	Myxococcus xanthus
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000663.5	0.55	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000646.5	0.46	terpene	β-carotein	uncultured bacterium
BGC0001456.3	0.45	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.44	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.44	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.43	terpene	carotenoid	Myxococcus xanthus
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

F5972_RS16280: PT_phytoene_like

Domain 1 (10 - 254): Prenyltransferase; Phytoene synthase like

Subtype: Hydroxysqualene synthase

Reactions:: PSPP → hydroxysqualene

F5972_RS16285: PT_phytoene_like

Domain 1 (54 - 372): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → PSPP; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

F5972_RS16295: T2TS

Domain 1 (7 - 620): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.02 out of 21.84

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (8):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.84 out of 27.78

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.68 out of 27.04

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.21 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.36 out of 29.38

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.70 out of 32.93

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.54 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.73 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.68 out of 29.65

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
MexT	Global virulence regulator

NZ_VYTZ01000005.1 - Region 3 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 462,361 - 483,428 nt. (total: 21,068 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

F5972_RS16370: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS16375: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

F5972_RS16410: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

F5972_RS16420: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: hydrolase

F5972_RS16450: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Ligases: synthetase

F5972_RS16415: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000249.5	0.42	PKS	nogalamycin	Streptomyces nogalater
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000665.5	0.41	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000679.5	0.40	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000905.5	0.40	other (other)	heme D1	Heliobacillus mobilis
BGC0001529.5	0.39	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0001374.5	0.39	other (other)	thienodolin	Streptomyces albogriseolus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000249.5	0.42	PKS	nogalamycin	Streptomyces nogalater
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000665.5	0.41	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000679.5	0.40	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000905.5	0.40	other (other)	heme D1	Heliobacillus mobilis
BGC0001529.5	0.39	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0001374.5	0.39	other (other)	thienodolin	Streptomyces albogriseolus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

F5972_RS16415: PT_FPPS_like

Domain 1 (64 - 353): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.47 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.19 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.71 out of 31.73

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.19 out of 29.51

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_VYTZ01000005.1 - Region 4 - azole-containing-RiPP,thioamitides

Download region GenBank file

Download region SVG

Location: 600,749 - 630,916 nt. (total: 30,168 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

F5972_RS17045: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS17055: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS37290: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: methyltransferase

F5972_RS17050: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002084.4	0.48	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0002099.3	0.48	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0001849.4	0.46	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001201.3	0.40	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0002560.3	0.38	ribosomal	thiostreptamide S4	Streptomyces sp. NRRL S-4
BGC0000844.5	0.37	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002127.3	0.33	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001802.5	0.32	ribosomal	thioholgamide A, thioholgamide B	Streptomyces malaysiense
BGC0001696.5	0.32	ribosomal	neothioviridamide	Streptomyces sp.
BGC0000625.5	0.32	ribosomal	thioviridamide	Streptomyces olivoviridis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002084.4	0.57	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0002099.3	0.57	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0001849.4	0.56	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001201.3	0.52	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0002560.3	0.51	ribosomal	thiostreptamide S4	Streptomyces sp. NRRL S-4
BGC0000844.5	0.50	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002127.3	0.48	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001802.5	0.47	ribosomal	thioholgamide A, thioholgamide B	Streptomyces malaysiense
BGC0001696.5	0.47	ribosomal	neothioviridamide	Streptomyces sp.
BGC0000625.5	0.47	ribosomal	thioviridamide	Streptomyces olivoviridis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (13):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.88 out of 30.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.65 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.31 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.77 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.09 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.82 out of 21.55

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.45 out of 18.57

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.42 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.30 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.31 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.89 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.87 out of 31.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.03 out of 29.65

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 1 - 89; Score: 46.6
: RRE type: Stand_Alone_Lasso_RRE; Location: 1 - 89; Score: 46.6

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
MexT	Global virulence regulator

Core peptide predictions

No core peptides found.

NZ_VYTZ01000006.1 - Region 1 - T3PKS,lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 109,423 - 150,511 nt. (total: 41,089 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

F5972_RS17740: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

F5972_RS17785: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

F5972_RS17840: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS17845: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS17855: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: methyltransferase

F5972_RS17745: Methylation

MITE: MITE0000155Methylation (identity: 68.40%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Ligases: ligase synthase

F5972_RS17680: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS17750: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

F5972_RS17770: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	1.14	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.64	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.59	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000513.3	0.55	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.54	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.53	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0001831.3	0.45	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000518.3	0.45	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000553.3	0.43	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002700.2	0.38	ribosomal	bacinapeptin	Bacillus nakamurai

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.82	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.63	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002276.2	0.58	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000518.3	0.56	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0001831.3	0.56	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000553.3	0.55	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for F5972_RS17730

F5972_RS17715

Leader:

MALLDLQTLEAPTGGGGGT

F5972_RS17720

Leader:

MALLDLQTLEAPTGGGGT

Shared core:

TGSNLSLLGCTPHENSSLSIICG

Core with dehydrations:

DhbGDhaNLDhaLLGCysDhbPHENDhaDhaLDhaIICysG

antiSMASH-DB 4.0 matches for F5972_RS17715

100.0%

NZ_VYTZ01000006.1and 1 other:

NZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17715_lanthipeptide

Lanthipeptides: F5972_RS17720_lanthipeptide

1

TGSNLSLLGCTPHENSSLSIICG

23

TGSNLSLLGCTPHENSSLSIICG

1

TGSNLSLLGCTPHENSSLSIICG

23

95.7%

NZ_BMMN01000011.1and 4 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42310_lanthipeptideNZ_JABBWY010000006.1 Lanthipeptides: HHX37_RS18535_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12965_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16255_lanthipeptide

Lanthipeptides: IEY80_RS26010_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLLGCTPHENSSLSIICG

1

GSNLSLLGCTPHENSSLSIICG

22

91.3%

NZ_QOIL01000022.1and 1 other:

NZ_QOIL01000022.1 Lanthipeptides: DQ384_RS31185_lanthipeptide

Lanthipeptides: DQ384_RS31190_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLLGCTPHENSSLSI+CG

1

GSNLSLLGCTPHENSSLSILCG

22

with 29 more sequence matches

Only the first 10 are displayed here.

87.5%

NZ_JABBWU010000019.1and 5 others:

NZ_JABBWU010000019.1 Lanthipeptides: HHX30_RS29850_lanthipeptideNZ_JABBWV010000002.1 Lanthipeptides: HHX31_RS05735_lanthipeptideNZ_JABBWX010000013.1 Lanthipeptides: HHX35_RS30145_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08060_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08055_lanthipeptide

Lanthipeptides: HHX30_RS29860_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLLGCTPHENSSLS+ICG

3

GSNLSLLGCTPHENSSLSLICG

24

87.0%

NZ_BMMN01000011.1and 1 other:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42315_lanthipeptide

Lanthipeptides: IEY80_RS26005_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLL CTPHENSSLS+ICG

1

GSNLSLLACTPHENSSLSLICG

22

87.0%

NZ_KI866522.1:

Lanthipeptides: MPTA5024_RS40525_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLLGCTPHENSSLS++CG

1

GSNLSLLGCTPHENSSLSVLCG

22

82.6%

NZ_JABBWU010000019.1:

Lanthipeptides: HHX30_RS29855_lanthipeptide

4

NLSLLGCTPHENSSLSIICG

23

N+SLLGCTPHENSSLSIICG

1

NVSLLGCTPHENSSLSIICG

20

82.6%

NZ_JABBWX010000013.1:

Lanthipeptides: HHX35_RS30140_lanthipeptide

4

NLSLLGCTPHENSSLSIICG

23

NLSLLGCTPHENSSLSI+CG

1

NLSLLGCTPHENSSLSIVCG

20

82.6%

NZ_JABBXA010000003.1:

Lanthipeptides: HHX41_RS08065_lanthipeptide

4

NLSLLGCTPHENSSLSIICG

23

NLSLLGCTPHENSSLS+ICG

1

NLSLLGCTPHENSSLSLICG

20

78.3%

NZ_BCBX01000043.1and 1 other:

NZ_BCBX01000043.1 Lanthipeptides: AP234_RS36585_lanthipeptide

Lanthipeptides: AP234_RS36580_lanthipeptide

2

GSNLSLLGCTPHENSSLSIICG

23

GSNLSLL C PHENSSLS++CG

1

GSNLSLLACVPHENSSLSLLCG

22

78.3%

NZ_JABBWV010000002.1:

Lanthipeptides: HHX31_RS05740_lanthipeptide

4

NLSLLGCTPHENSSLSIICG

23

NLSLLGCTPHENSSLS++CG

1

NLSLLGCTPHENSSLSLVCG

20

73.9%

NZ_JABBWY010000006.1and 3 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12960_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16250_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17725_lanthipeptide

Lanthipeptides: HHX37_RS18530_lanthipeptide

5

LSLLGCTPHENSSLSIICG

23

LSLL CTPHENSSLS+ICG

1

LSLLACTPHENSSLSLICG

19

52.2%

NZ_BIFH01000016.1:

Lanthipeptides: EMA17_RS12985_lanthipeptide

2

GSNLSLLGCTPHENSSLSII

21

GSN+SLL C NSSLS++

3

GSNVSLLLCG---NSSLSLL

19

MIBiG 4.0 matches for F5972_RS17715

52.2%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

1

TGSNLSLLGCTPHENSSLSII

21

TGS +SLL C E SSLS++

1

TGSQVSLLVC---EYSSLSVV

18

F5972_RS17725

Leader:

MALLDLQTLEAPGGHGGGGGSN

Core:

LSLLACTPHENSSLSLICG

Core with dehydrations:

LDhaLLACysDhbPHENDhaDhaLDhaLICysG

antiSMASH-DB 4.0 matches for F5972_RS17725

86.4%

NZ_BMMN01000011.1and 5 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42315_lanthipeptideNZ_JABBWY010000006.1 Lanthipeptides: HHX37_RS18530_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12960_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16250_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17725_lanthipeptide

Lanthipeptides: IEY80_RS26005_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLLACTPHENSSLSLICG

4

LSLLACTPHENSSLSLICG

22

85.0%

NZ_JABBWV010000002.1:

Lanthipeptides: HHX31_RS05740_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLSL+CG

2

LSLLGCTPHENSSLSLVCG

20

80.0%

NZ_JABBWU010000019.1:

Lanthipeptides: HHX30_RS29855_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

+SLL CTPHENSSLS+ICG

2

VSLLGCTPHENSSLSIICG

20

with 16 more sequence matches

Only the first 10 are displayed here.

80.0%

NZ_JABBWX010000013.1:

Lanthipeptides: HHX35_RS30140_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

2

LSLLGCTPHENSSLSIVCG

20

77.3%

NZ_BCBX01000043.1and 1 other:

NZ_BCBX01000043.1 Lanthipeptides: AP234_RS36585_lanthipeptide

Lanthipeptides: AP234_RS36580_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLLAC PHENSSLSL+CG

4

LSLLACVPHENSSLSLLCG

22

77.3%

NZ_BMMN01000011.1and 6 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42310_lanthipeptideNZ_JABBWY010000006.1 Lanthipeptides: HHX37_RS18535_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12965_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16255_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17720_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17715_lanthipeptide

Lanthipeptides: IEY80_RS26010_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS+ICG

4

LSLLGCTPHENSSLSIICG

22

75.0%

NZ_JABBWU010000019.1and 6 others:

NZ_JABBWU010000019.1 Lanthipeptides: HHX30_RS29850_lanthipeptideNZ_JABBWV010000002.1 Lanthipeptides: HHX31_RS05735_lanthipeptideNZ_JABBWX010000013.1 Lanthipeptides: HHX35_RS30145_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08060_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08055_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08065_lanthipeptide

Lanthipeptides: HHX30_RS29860_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLSLICG

6

LSLLGCTPHENSSLSLICG

24

69.6%

NZ_KI866522.1:

Lanthipeptides: MPTA5024_RS40525_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

4

LSLLGCTPHENSSLSVLCG

22

69.6%

NZ_QOIL01000022.1and 1 other:

NZ_QOIL01000022.1 Lanthipeptides: DQ384_RS31185_lanthipeptide

Lanthipeptides: DQ384_RS31190_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

4

LSLLGCTPHENSSLSILCG

22

50.0%

NZ_AUBE01000003.1:

Lanthipeptides: G412_RS38950_lanthipeptide

1

LSLLACTPHENSSLSLIC

18

LSLLAC H ++S L+C

3

LSLLACDKHSSNSF-LLC

19

45.5%

NZ_CM000913.1and 1 other:

NZ_CP016559.1 Lanthipeptides: BB341_RS04185_lanthipeptide

Lanthipeptides: SCLAV_RS23790_lanthipeptide

2

SLLACTPHENSSLSLIC

18

SLL C PH + SL L+C

6

SLLVCGPHSSQSL-LLC

21

42.9%

NZ_CP042266.1:

Lanthipeptides: FQU76_RS00910_lanthipeptide

1

LSLLACTPHENSSL

14

LSLL+C H N SL

5

LSLLSCGHHSNLSL

18

38.1%

NZ_BMQJ01000002.1:

Lanthipeptides: IEZ10_RS04315_lanthipeptide

1

LSLLACTPHENSSLSLI

17

L++L C H S+LSL+

2

LTVLGCESHTPSNLSLL

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (24):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.00 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.42 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.97 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.28 out of 21.55

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.43 out of 36.54

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.49 out of 30.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.87 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.66 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.65 out of 32.93

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.04 out of 29.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.26 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.52 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.63 out of 18.57

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.14 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.53 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.63 out of 18.57

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.35 out of 25.88

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.22 out of 29.25

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.52 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.48 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

F5972_RS17715 - Class III

Cleavage pHMM score: 10.40

RODEO score: 0

Monoisotopic mass: 2210.1 Da

Molecular weight: 2211.4 Da

Number of bridges: 2

Additional modifications: Cl

Alternative weights: (assuming N unmodified Ser/Thr residues); 2229.4 Da N = 1; 2247.5 Da N = 2; 2265.5 Da N = 3; 2283.5 Da N = 4; 2301.5 Da N = 5

F5972_RS17720 - Class III

Cleavage pHMM score: 11.00

RODEO score: 0

Monoisotopic mass: 2210.1 Da

Molecular weight: 2211.4 Da

Number of bridges: 2

Additional modifications: Cl

Alternative weights: (assuming N unmodified Ser/Thr residues); 2229.4 Da N = 1; 2247.5 Da N = 2; 2265.5 Da N = 3; 2283.5 Da N = 4; 2301.5 Da N = 5

F5972_RS17725 - Class III

Cleavage pHMM score: 14.60

RODEO score: 0

Monoisotopic mass: 1901.0 Da

Molecular weight: 1902.2 Da

Number of bridges: 2

Additional modifications: Cl

Alternative weights: (assuming N unmodified Ser/Thr residues); 1920.2 Da N = 1; 1938.2 Da N = 2; 1956.2 Da N = 3

NRPS/PKS substrate predictions

F5972_RS17680: X

AMP-binding (24..487): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EATLHIARALSLFGMAAGEIDVYGMTETTGAFTV
Nearest Stachelhaus code(s):: Trp GALFFAAGVK (41% 8Å match); Ile DALFMGVVFK (35% 8Å match); Leu DALFMGAVAK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_VYTZ01000006.1 - Region 2 - T1PKS,other

Download region GenBank file

Download region SVG

Location: 236,548 - 327,175 nt. (total: 90,628 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

F5972_RS18285: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS18295: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS18335: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

F5972_RS18385: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

F5972_RS18390: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

F5972_RS18400: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS18440: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

F5972_RS18480: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

F5972_RS18485: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

F5972_RS18525: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

F5972_RS18530: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Transferases: acylase aminotransferase glycosyltransferase transferase

F5972_RS18290: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

F5972_RS18305: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS18340: aminotransferase

smcogs: SMCOG1019: aminotransferase

F5972_RS18395: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

F5972_RS18405: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

F5972_RS18430: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

F5972_RS18470: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

F5972_RS18545: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: peptidase

F5972_RS18360: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Lyases: dehydratase

F5972_RS18315: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

F5972_RS18420: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

F5972_RS18300: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS18310: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001817.5	0.83	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001254.3	0.53	PKS	ACT-Toxin II	Alternaria alternata
BGC0000056.3	0.53	PKS	esperamicin	Actinomadura verrucosospora
BGC0000827.3	0.50	other (other)	staurosporine	Salinispora arenicola CNS-205
BGC0002154.2	0.49	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001258.3	0.49	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.49	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002047.2	0.48	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis
BGC0000813.3	0.47	other (other)	K-252a	Nonomuraea longicatena

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000056.3	0.70	PKS	esperamicin	Actinomadura verrucosospora
BGC0001164.3	0.69	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.68	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001817.5	0.65	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (25):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.44 out of 25.88

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.66 out of 21.55

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.22 out of 26.85

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.93 out of 20.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.12 out of 27.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.62 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.46 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.99 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.46 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.49 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.19 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.52 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.89 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.39 out of 20.01

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.72 out of 32.74

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.71 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.60 out of 21.84

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.71 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.68 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.68 out of 28.19

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.10 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 21.93 out of 30.11

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.70 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.90 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 236547 - 327175:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 236547 - 281794:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS18300: NH2

CAL_domain (40..486): NH2

minowa: NH2

Prediction, score:

NH2: 100.0
AHBA: 13.3
shikimic_acid: 6.1
Acetyl-CoA: 2.7
fatty_acid: -2.1

F5972_RS18305: mmal

PKS_AT (660..945): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 118.9
Methoxymalonyl-CoA: 113.0
Ethylmalonyl-CoA: 112.3
Malonyl-CoA: 92.2
2-Methylbutyryl-CoA: 66.9
trans-1,2-CPDA: 65.2
Isobutyryl-CoA: 64.3
Benzoyl-CoA: 58.7
Propionyl-CoA: 57.4
fatty_acid: 47.6
Acetyl-CoA: 43.3
CHC-CoA: 40.6
inactive: 22.5
3-Methylbutyryl-CoA: 19.5

F5972_RS18470: mal

PKS_AT (623..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 92.7
Methoxymalonyl-CoA: 53.1
Methylmalonyl-CoA: 45.5
inactive: 33.5
Propionyl-CoA: 25.0
Ethylmalonyl-CoA: 23.0
fatty_acid: 14.3
Isobutyryl-CoA: 14.3
2-Methylbutyryl-CoA: 10.1
trans-1,2-CPDA: 9.9
CHC-CoA: 9.0
Benzoyl-CoA: 8.8
Acetyl-CoA: 4.1
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_VYTZ01000006.1 - Region 3 - NRPS

Download region GenBank file

Download region SVG

Location: 410,391 - 468,763 nt. (total: 58,373 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

F5972_RS18995: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

F5972_RS19110: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: hydrolase

F5972_RS19140: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

F5972_RS19020: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

F5972_RS19030: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS19035: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS19040: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS19050: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS19060: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

F5972_RS19065: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS19070: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transporter

F5972_RS19095: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001050.5	0.71	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001050.5	0.71	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.08 out of 18.57

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.15 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.19 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.39 out of 30.11

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.20 out of 23.43

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (14):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.71 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.05 out of 27.55

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.88 out of 33.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.86 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.73 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.88 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.07 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.09 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.20 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.41 out of 33.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.57 out of 24.78

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.94 out of 23.43

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 7, location 410390 - 468763:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Gly) + (Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS19030: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (517..939): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVQFDFGVTVLKILLGGEVNHYGPTEATVGVTT
Nearest Stachelhaus code(s):: Asp DLTKIGHVGK (74% 8Å match); Asn DLTKLGEVGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

F5972_RS19035: X

AMP-binding (21..420): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: CQPFTNAGQTMNFLGSAAATNYYTSTEAAPAQTI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS19065: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (29..426): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: LSFTFDISVLELQALCGGEWNVYGPTETTVWSTA
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

F5972_RS19070: X

AMP-binding (460..891): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMAFDTHVEEPDLIIGGELNTYGPTEAAVIATW
Nearest Stachelhaus code(s):: Lys DTCDIGTVIK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AbrC3	Antibiotic production activator
BldD	Development and antibiotic global regulator
CelR	Cellobiose uptake repressor
ToxT	Regulator of MSHA biosynthesis

NZ_VYTZ01000007.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 128,328 - 153,210 nt. (total: 24,883 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase sulfotransferase

F5972_RS20430: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

F5972_RS20495: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

F5972_RS20450: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

F5972_RS20460: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.75	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.57	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.50	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001911.3	0.45	terpene	raimonol	Streptomyces anulatus
BGC0001910.4	0.43	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000496.5	0.41	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000927.3	0.41	other (flavin)	roseoflavin	Streptomyces davaonensis JCM 4913

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.75	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.57	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.50	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001911.3	0.45	terpene	raimonol	Streptomyces anulatus
BGC0001910.4	0.43	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000496.5	0.41	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000927.3	0.41	other (flavin)	roseoflavin	Streptomyces davaonensis JCM 4913

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

F5972_RS20450: T1TS + T1TS

Domain 1 (2 - 321): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (340 - 685): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

F5972_RS20460: PT_FPPS_like

Domain 1 (52 - 356): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (7):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.22 out of 30.22

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.45 out of 25.75

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.74 out of 29.72

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.31 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.90 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.70 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.19 out of 20.97

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_VYTZ01000007.1 - Region 2 - NAPAA

Download region GenBank file

Download region SVG

Location: 156,414 - 190,478 nt. (total: 34,065 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS20625: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Hydrolases: hydrolase

F5972_RS20540: isochorismatase

smcogs: SMCOG1027: isochorismatase

Ligases: ligase

F5972_RS20600: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transporter

F5972_RS20635: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.62 out of 19.52

Confidence: medium

Additional weak hits (12):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.23 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.88 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.08 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.33 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.99 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.79 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.79 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.37 out of 21.55

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.61 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.41 out of 30.11

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.15 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 156413 - 190478:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS20600: X

AMP-binding (28..423): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FVVMFDFSVEEPYCLVGGELNTYGPTEATVTATC
Nearest Stachelhaus code(s):: Lys DIESIGTVTK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_VYTZ01000007.1 - Region 3 - NRPS

Download region GenBank file

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Location: 301,558 - 347,092 nt. (total: 45,535 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

F5972_RS21220: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

F5972_RS21225: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS21240: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

F5972_RS21295: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

F5972_RS21330: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS21335: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

F5972_RS21340: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: glycosyltransferase

F5972_RS21230: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: hydrolase phosphatase

F5972_RS21215: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

F5972_RS21235: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Ligases: ligase

F5972_RS21245: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.52 out of 27.55

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.37 out of 26.85

Confidence: medium

Additional weak hits (26):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.17 out of 29.51

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.34 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.23 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.32 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.79 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.13 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.38 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.53 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.55 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.44 out of 36.54

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.69 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.81 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.98 out of 30.22

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.67 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.32 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.49 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.65 out of 21.55

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.05 out of 27.55

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.33 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.90 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.83 out of 25.88

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.51 out of 26.85

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 301557 - 347092:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS21245: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (508..906): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWAGFDLVVPNAKCVVAGEVNEYGPTEASVGTCI
Nearest Stachelhaus code(s):: D-Ala DLTKVNEVGK (71% 8Å match); Asn DLTKVGEVGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CelR	Cellobiose uptake repressor
GnfM	Regulator of nitrogen-fixation genes
RutR	Regulator of the pyrimidine and purine metabolism

NZ_VYTZ01000008.1 - Region 1 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 33,423 - 58,545 nt. (total: 25,123 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

F5972_RS22695: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

F5972_RS22700: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.44	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000541.5	0.44	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0001727.3	0.40	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000721.4	0.39	saccharide	tobramycin	Streptoalloteichus tenebrarius
BGC0000529.5	0.37	ribosomal	microbisporicin A2	Microbispora corallina
BGC0000288.5	0.25	NRPS	A-503083 A, A-503083 B, A-503083 E, A-503083 F	Streptomyces sp. SANK 62799

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.44	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000541.5	0.44	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0001727.3	0.40	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000721.4	0.39	saccharide	tobramycin	Streptoalloteichus tenebrarius
BGC0000529.5	0.37	ribosomal	microbisporicin A2	Microbispora corallina
BGC0000288.5	0.25	NRPS	A-503083 A, A-503083 B, A-503083 E, A-503083 F	Streptomyces sp. SANK 62799

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for F5972_RS22690

F5972_RS22705

Leader:

MSIQIENAPATQAGEEDFGEWDLDVSIVESGPAAD

Core:

KLIRMTDDGCGTSCQSACPATCP

Core with dehydrations:

KLIRMDhbDDGCysGDhbDhaCysQDhaACysPADhbCysP

antiSMASH-DB 4.0 matches for F5972_RS22705

100.0%

NZ_JABBWZ010000004.1and 4 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptide

Lanthipeptides: HHX40_RS10860_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

KLIRMTDDGCGTSCQSACPATCP

1

KLIRMTDDGCGTSCQSACPATCP

23

87.0%

NZ_BMMN01000001.1and 1 other:

NZ_BMMN01000001.1 Lanthipeptides: IEY80_RS02165_lanthipeptide

Lanthipeptides: IEY80_RS02165_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

KLIRMTDDGCGT+CQSAC TCP

1

KLIRMTDDGCGTTCQSACSTTCP

23

78.3%

NZ_CP047146.1and 1 other:

NZ_CP047146.1 Lanthipeptides: GR130_RS38445_lanthipeptide

Lanthipeptides: GR130_RS38445_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC ATCP

1

RLIRMTDDGCGQTCESACSATCP

23

with 180 more sequence matches

Only the first 10 are displayed here.

78.3%

NZ_CP054916.1and 1 other:

NZ_CP054916.1 Lanthipeptides: HUT19_RS32830_lanthipeptide

Lanthipeptides: HUT19_RS32830_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +CQSAC TCP

1

RLIRMTDDGCGQTCQSACSTTCP

23

78.3%

NZ_WBMT01000015.1and 1 other:

NZ_WBMT01000015.1 Lanthipeptides: F8566_RS30065_lanthipeptide

Lanthipeptides: F8566_RS30065_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATC

22

+LIRMTDDGCG +CQSACP TC

1

RLIRMTDDGCGKTCQSACPNTC

22

73.9%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCGT+C++AC TCP

1

RLIRMTDDGCGTTCETACSTTCP

23

73.9%

NZ_CM000913.1and 7 others:

NZ_CM000913.1 Lanthipeptides: SCLAV_RS11700_lanthipeptideNZ_CP016559.1 Lanthipeptides: BB341_RS16135_lanthipeptideNZ_CP016559.1 Lanthipeptides: BB341_RS16135_lanthipeptideNZ_CP042266.1 Lanthipeptides: FQU76_RS24360_lanthipeptideNZ_CP042266.1 Lanthipeptides: FQU76_RS24360_lanthipeptideNZ_CP070379.1 Lanthipeptides: JVE63_RS07215_lanthipeptideNZ_CP070379.1 Lanthipeptides: JVE63_RS07215_lanthipeptide

Lanthipeptides: SCLAV_RS11700_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC TCP

1

RLIRMTDDGCGVTCESACSTTCP

23

73.9%

NZ_CP048875.1and 1 other:

NZ_CP048875.1 Lanthipeptides: G3260_RS00640_lanthipeptide

Lanthipeptides: G3260_RS00640_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG +C+SAC ATCP

1

RLIQMTDDGCGQTCESACSATCP

23

73.9%

NZ_CP119145.1and 1 other:

NZ_CP119145.1 Lanthipeptides: PZB75_RS00285_lanthipeptide

Lanthipeptides: PZB75_RS00285_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG++CQSAC TCP

1

RLIQMTDDGCGSTCQSACSTTCP

23

73.9%

NZ_FOLM01000022.1and 1 other:

NZ_FOLM01000022.1 Lanthipeptides: BM242_RS25935_lanthipeptide

Lanthipeptides: BM242_RS25935_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC TCP

1

RLIRMTDDGCGETCESACSTTCP

23

71.4%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS09160_lanthipeptide

Lanthipeptides: FRANCCI3_RS09160_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCGT+CQSACP TCP

4

ELIRMTDDGCGTTCQSACPNTCP

26

69.6%

NZ_JACHGT010000013.1and 1 other:

NZ_JACHGT010000013.1 Lanthipeptides: HNR73_RS27205_lanthipeptide

Lanthipeptides: HNR73_RS27205_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI MT+DGCG +CQSAC TCP

1

RLIHMTNDGCGQTCQSACSTTCP

23

69.6%

NZ_JAFIQY010000004.1and 1 other:

NZ_JAFIQY010000004.1 Lanthipeptides: JXZ78_RS08025_lanthipeptide

Lanthipeptides: JXZ78_RS08025_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG +C+SAC TCP

1

RLIQMTDDGCGETCESACSTTCP

23

MIBiG 4.0 matches for F5972_RS22705

15.4%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

12

TSCQSAC

18

T C S C

20

TKCTSKC

26

10.8%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

12

TSCQSAC

18

T C S C

31

TKCTSRC

37

Lanthipeptide(s) for F5972_RS22700

F5972_RS22705

Leader:

MSIQIENAPATQAGEEDFGEWDLDVSIVESGPAAD

Core:

KLIRMTDDGCGTSCQSACPATCP

Core with dehydrations:

KLIRMDhbDDGCysGDhbDhaCysQDhaACysPADhbCysP

antiSMASH-DB 4.0 matches for F5972_RS22705

100.0%

NZ_JABBWZ010000004.1and 4 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptide

Lanthipeptides: HHX40_RS10860_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

KLIRMTDDGCGTSCQSACPATCP

1

KLIRMTDDGCGTSCQSACPATCP

23

87.0%

NZ_BMMN01000001.1and 1 other:

NZ_BMMN01000001.1 Lanthipeptides: IEY80_RS02165_lanthipeptide

Lanthipeptides: IEY80_RS02165_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

KLIRMTDDGCGT+CQSAC TCP

1

KLIRMTDDGCGTTCQSACSTTCP

23

78.3%

NZ_CP047146.1and 1 other:

NZ_CP047146.1 Lanthipeptides: GR130_RS38445_lanthipeptide

Lanthipeptides: GR130_RS38445_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC ATCP

1

RLIRMTDDGCGQTCESACSATCP

23

with 180 more sequence matches

Only the first 10 are displayed here.

78.3%

NZ_CP054916.1and 1 other:

NZ_CP054916.1 Lanthipeptides: HUT19_RS32830_lanthipeptide

Lanthipeptides: HUT19_RS32830_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +CQSAC TCP

1

RLIRMTDDGCGQTCQSACSTTCP

23

78.3%

NZ_WBMT01000015.1and 1 other:

NZ_WBMT01000015.1 Lanthipeptides: F8566_RS30065_lanthipeptide

Lanthipeptides: F8566_RS30065_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATC

22

+LIRMTDDGCG +CQSACP TC

1

RLIRMTDDGCGKTCQSACPNTC

22

73.9%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCGT+C++AC TCP

1

RLIRMTDDGCGTTCETACSTTCP

23

73.9%

NZ_CM000913.1and 7 others:

NZ_CM000913.1 Lanthipeptides: SCLAV_RS11700_lanthipeptideNZ_CP016559.1 Lanthipeptides: BB341_RS16135_lanthipeptideNZ_CP016559.1 Lanthipeptides: BB341_RS16135_lanthipeptideNZ_CP042266.1 Lanthipeptides: FQU76_RS24360_lanthipeptideNZ_CP042266.1 Lanthipeptides: FQU76_RS24360_lanthipeptideNZ_CP070379.1 Lanthipeptides: JVE63_RS07215_lanthipeptideNZ_CP070379.1 Lanthipeptides: JVE63_RS07215_lanthipeptide

Lanthipeptides: SCLAV_RS11700_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC TCP

1

RLIRMTDDGCGVTCESACSTTCP

23

73.9%

NZ_CP048875.1and 1 other:

NZ_CP048875.1 Lanthipeptides: G3260_RS00640_lanthipeptide

Lanthipeptides: G3260_RS00640_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG +C+SAC ATCP

1

RLIQMTDDGCGQTCESACSATCP

23

73.9%

NZ_CP119145.1and 1 other:

NZ_CP119145.1 Lanthipeptides: PZB75_RS00285_lanthipeptide

Lanthipeptides: PZB75_RS00285_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG++CQSAC TCP

1

RLIQMTDDGCGSTCQSACSTTCP

23

73.9%

NZ_FOLM01000022.1and 1 other:

NZ_FOLM01000022.1 Lanthipeptides: BM242_RS25935_lanthipeptide

Lanthipeptides: BM242_RS25935_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCG +C+SAC TCP

1

RLIRMTDDGCGETCESACSTTCP

23

71.4%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS09160_lanthipeptide

Lanthipeptides: FRANCCI3_RS09160_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LIRMTDDGCGT+CQSACP TCP

4

ELIRMTDDGCGTTCQSACPNTCP

26

69.6%

NZ_JACHGT010000013.1and 1 other:

NZ_JACHGT010000013.1 Lanthipeptides: HNR73_RS27205_lanthipeptide

Lanthipeptides: HNR73_RS27205_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI MT+DGCG +CQSAC TCP

1

RLIHMTNDGCGQTCQSACSTTCP

23

69.6%

NZ_JAFIQY010000004.1and 1 other:

NZ_JAFIQY010000004.1 Lanthipeptides: JXZ78_RS08025_lanthipeptide

Lanthipeptides: JXZ78_RS08025_lanthipeptide

1

KLIRMTDDGCGTSCQSACPATCP

23

+LI+MTDDGCG +C+SAC TCP

1

RLIQMTDDGCGETCESACSTTCP

23

MIBiG 4.0 matches for F5972_RS22705

15.4%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

12

TSCQSAC

18

T C S C

20

TKCTSKC

26

10.8%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

12

TSCQSAC

18

T C S C

31

TKCTSRC

37

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.61 out of 30.11

Confidence: medium

Additional weak hits (8):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.98 out of 29.25

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.13 out of 29.51

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.54 out of 39.62

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.04 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.32 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.20 out of 21.55

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.41 out of 30.11

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

F5972_RS22705 - Class I

Cleavage pHMM score: -4.70

RODEO score: 19

Monoisotopic mass: 2267.0 Da

Molecular weight: 2268.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2286.6 Da N = 1

F5972_RS22705 - Class I

Cleavage pHMM score: -4.70

RODEO score: 19

Monoisotopic mass: 2267.0 Da

Molecular weight: 2268.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2286.6 Da N = 1

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 152 - 243; Score: 71.9

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CelR	Cellobiose uptake repressor

NZ_VYTZ01000008.1 - Region 2 - RiPP-like

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Location: 177,179 - 187,448 nt. (total: 10,270 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

F5972_RS23380: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0001652.5		0.19	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0001652.5		0.19	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (3):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.88 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.65 out of 27.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.88 out of 35.63

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_VYTZ01000008.1 - Region 3 - RRE-containing

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Location: 224,420 - 245,541 nt. (total: 21,122 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

F5972_RS23670: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.45	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000585.5	0.44	ribosomal	microcin C7	Escherichia coli
BGC0001633.3	0.36	other (other)	ketomemicin B3, ketomemicin B4	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000812.3	0.35	other (other)	huperzine A	Shiraia sp. slf14
BGC0001187.4	0.34	NRPS, PKS	xenolozoyenone	Glarea lozoyensis
BGC0000041.3	0.33	PKS	oronofacic acid	Pseudomonas syringae
BGC0001858.2	0.32	PKS	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000353.5	0.31	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0001221.5	0.30	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000925.5	0.29	other (other)	quinolobactin	Pseudomonas fluorescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.45	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000585.5	0.44	ribosomal	microcin C7	Escherichia coli
BGC0001633.3	0.36	other (other)	ketomemicin B3, ketomemicin B4	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000812.3	0.35	other (other)	huperzine A	Shiraia sp. slf14
BGC0001187.4	0.34	NRPS, PKS	xenolozoyenone	Glarea lozoyensis
BGC0000041.3	0.33	PKS	oronofacic acid	Pseudomonas syringae
BGC0001858.2	0.32	PKS	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000353.5	0.31	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0001221.5	0.30	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000925.5	0.29	other (other)	quinolobactin	Pseudomonas fluorescens

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.59 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.27 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.28 out of 27.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.31 out of 25.88

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.00 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.85 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.89 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.21 out of 33.14

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 94; Score: 61.3

TFBS definitions

Regulator	Description

NZ_VYTZ01000009.1 - Region 1 - T1PKS

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Location: 131,384 - 179,195 nt. (total: 47,812 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: dehalogenase protease

F5972_RS25215: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

F5972_RS25220: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: dehydratase

F5972_RS25225: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

F5972_RS25230: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

F5972_RS25235: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

F5972_RS25240: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.52 out of 30.11

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.38 out of 23.79

Confidence: medium

Additional weak hits (12):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.85 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.82 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.13 out of 26.33

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.04 out of 30.11

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.44 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.79 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.65 out of 26.12

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.72 out of 26.12

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.74 out of 31.73

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.10 out of 30.11

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.36 out of 31.73

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 131383 - 179195:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

F5972_RS25230: X

AMP-binding (24..443): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QLLF-----PFGFAVSAGALSVYGATECMPVAAI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

F5972_RS25240: pk

PKS_AT (572..867): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; CE-Malonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 109.5
Methoxymalonyl-CoA: 95.3
Malonyl-CoA: 90.6
Isobutyryl-CoA: 69.4
Ethylmalonyl-CoA: 67.5
CHC-CoA: 56.5
trans-1,2-CPDA: 42.1
Propionyl-CoA: 39.4
Benzoyl-CoA: 29.2
2-Methylbutyryl-CoA: 25.9
inactive: 25.3
fatty_acid: 21.4
Acetyl-CoA: 10.1
3-Methylbutyryl-CoA: 8.1

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CelR	Cellobiose uptake repressor
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems