LOCUS       NZ_VYTZ01000030        10302 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Microbispora cellulosiformans strain Gxj-6 Scaffold30, whole genome
            shotgun sequence.
ACCESSION   NZ_VYTZ01000030
VERSION     NZ_VYTZ01000030.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora cellulosiformans
  ORGANISM  Microbispora cellulosiformans
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Gong,X.
  TITLE     Screening of Novel Bioactive Compounds from Soil-Associated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   Gong,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) College of Land and Environment, Shenyang
            Agricultural University, No. 120, Dongling Road, Shenhe District,
            Shenyang, Liao Ning 110866, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008728085.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 02:05:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,471
            CDSs (total)                      :: 7,401
            Genes (coding)                    :: 7,312
            CDSs (with protein)               :: 7,312
            Genes (RNA)                       :: 70
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 7 of 89
            Pseudo Genes (incomplete)         :: 80 of 89
            Pseudo Genes (internal stop)      :: 8 of 89
            Pseudo Genes (multiple problems)  :: 6 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:06:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10302
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VYTZ01000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10302
                     /collection_date="2015-04-30"
                     /db_xref="taxon:2614688"
                     /geo_loc_name="China: Heilongjiang Province, Heihe City"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Microbispora cellulosiformans"
                     /strain="Gxj-6"
                     /submitter_seqid="Scaffold30"
                     /type_material="type strain of Microbispora
                     cellulosiformans"
     region          1..10302
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..10302
                     /SMILES="C=CC=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10302
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<5:9755](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <6..9755
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            <6..9755
                     /locus_tag="F5972_RS36540"
                     /old_locus_tag="F5972_36545"
                     /pseudo=""
     CDS             <6..9755
                     /NRPS_PKS="Domain: PKS_AT (191-481). E-value: 2.8e-110.
                     Score: 360.3. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (560-730). E-value: 2.8e-62.
                     Score: 201.9. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1028-1207). E-value: 2.4e-59.
                     Score: 192.3. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1310-1382). E-value: 2.7e-28.
                     Score: 90.1. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1404-1815). E-value:
                     3.9e-178. Score: 584.6. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1996-2283). E-value: 8.7e-108.
                     Score: 352.1. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_AT.2"
                     /NRPS_PKS="Domain: PKS_DH (2356-2526). E-value: 2.7e-59.
                     Score: 192.2. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_DH.2"
                     /NRPS_PKS="Domain: PKS_KR (2816-2991). E-value: 5.4e-53.
                     Score: 171.6. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_KR.2"
                     /NRPS_PKS="Domain: PKS_PP (3078-3164). E-value: 3.8e-28.
                     Score: 89.7. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (3176-3240). E-value:
                     1.5e-09. Score: 30.5. Matches aSDomain:
                     nrpspksdomains_F5972_RS36540_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048599.1"
                     /locus_tag="F5972_RS36540"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_36545"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /pseudo=""
                     /sec_met_domain="PKS_AT (E-value: 9.3e-112, bitscore:
                     360.3, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 3.1e-63, bitscore:
                     200.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5e-15, bitscore:
                     43.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1e-222, bitscore: 726.6,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 5.3e-87, bitscore:
                     278.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LLTEAMPWPEVDRPRRAGVSSFGISGTNAHVIIEQAPADLAAEVT
                     LTEAPAPALLVGTVDPATAEVADATALALAESGQPAGAAVAGEGATQAGAPDTSVVPVA
                     WVLSGKTPDALAGQAARVLSFVQDRPALSVADVGLSLATTRASFERRAVVTGADRAALL
                     AGLAALAEGRTAPGAVQGVARSAGKLGVLFTGQGAQRLGMGRELHAAFPVFAEAFDAVV
                     AELDAHLDRPLREVIWGADAETVDQTAYAQAGLFAVEVALFRLLTSWGVRPDYLAGHSV
                     GELAAAHVAGMLTLSDACRLVAARGRLMQALPSGGAMVAVQATEDEVLPLLTDGVDIAA
                     INGPRAVVVSGQEAPVLALAERLAEQGRKTRRLRVSHAFHSHLMEPMLADFAEVAESLT
                     YGSASIPIVSTVTGGLADEAFGTPEYWVGQVRGAVRFADAVTTLAAQGVTRFVELGPDA
                     VLTAMAQQTLDEAADTAVFTATMRAERPEPGTVVTALGQLHTAGVPVDWQAFYAETGAR
                     QVDLPTYAFQRERYWLNPIDYLSESWVADHIGNLSSAGLEAVEHPLLGAVLPLPESGGV
                     VFTGQLSVRSHPWLADHQVFGSVLLPGTGLVELALHAAEHTGCTLVEELTLAAPLVLPG
                     GAAGSGEGAVQVQVVVGAADGDGRRTVAIYSRGAQAPADLPWTLHAEGVLASETAQNGA
                     DLSQWPPAEATAVDLTDAYAILNERGYGYGPAFQGLQAAWRRGEELYAEVALPDQEHQD
                     AALFGLHPALFDAALHALHFGGDDTDGGPTMLPFSWAGVSLSAVGATALRVRLTPAGPN
                     AFSLEIADAEGAPVAAVRSLTSRPVAPEQLQPARLHDSLYRVDWTKVPVPPAVSLTYAD
                     WAALDPAAATVPDVIVLRVPSGDPVGASADEAAAVRATTHQVLAALQEWTAGERFADSR
                     LLVVTRGAVAPLSGSDDVTDLAGAAVWGLVRTAQREHPDRIVLADLHESADESVDVGAV
                     MAVGEPQVALRDGAAYAPRLVRVPAATTEAEPVTSFPAEGTVLVTGGTGMLGRLVARHL
                     VAAHGVRRLLLTSRSASAESAADLVGELADLGAEVSVAAADAADRASLAGVLAGIPAEH
                     PLVGVVHLAGVLDDGVIASLTPERMDRVLRPKVDAALNLHELTRDHDLAAFVLFSSASG
                     VFGTPGQGSYAAANAFLDALAAHRRAGGLTAQSLAWGLWADDAGMAGELGDTDRQRLSR
                     NGVEPLSAQQGLELFDVACTVDSGTLVPIRLDFAALAEAGAELPSLFRTLVRVPTRRVA
                     AAGGGASSAVRERFAGLTAAERVAAALDIVRTQAAAVLGHAGVEAIEPDRAFGELGFDS
                     LSAVELRNGLNAATGLRLPASLVFDYPNALAVAEHIADEVVGADTRRTVVAATAGSVDE
                     PLAIVGMACRYPGGVSSPEDLWRLVAGGVDAISDLPVNRGWNIEEIFDPEGKRPNTSYV
                     KSGGFLHEAAEFDAAFFGISPNEALTMDPQQRLLLETSWEAMERAGIDPGSLRGSSTGV
                     FAGMMYHDYAANANTGSIASGRVSYVFGFEGPSVTIDTACSSSLVALHLAGQALRSGEC
                     SLALVGGVAVMATPDTFIEFSRQRGLSPDGRCRAYGAGANGTAWAEGCGVLLVERLSDA
                     RRLGHRVLAVVRGTAVNQDGASNGLTAPNGPSQERVIRQALANARLSVSDVDVVEGHGT
                     GTTLGDPIEAQAVLATYGQDRPEEQPLWLGSLKSNIGHAQAAAGVGGIIKMVMAMHNGV
                     LPKTLHADEPSPHVEWSAGHVRLLTEAMPWPEQDRPRRAGVSSFGISGTNAHVIIEQAP
                     ADLAADVLVAEAPAPAVLTGTALPATAVAEVTALVPGDGVSSGPIPAMAELAVAADGAA
                     PVLAESGQPAGAAVAGEGAALVPAGSAAQAGAPVAWVVSGKTADALAGQAARVLSYVQD
                     RPSLSLADVGFSLATTRAAFERRAVVTGGDRAALLDGLAALAEGRKAPGVVQGVARSSG
                     KVGVLFTGQGAQRLGMGRELHAAFPVFADAFDAVAGELDAHLDRPLREVIWGADPVLAD
                     QTAYAQAGLFAVEVALFRLVSSWGVRPDYLAGHSVGELAAAHVAGMLTLADASRLVAAR
                     GRLMQALPAGGAMVAVQATEDEVTPLLTDGVDIAAINGPRAVVVSGQEGPVLALAERLA
                     EQGRKTRRLRVSHAFHSHLMEPMLADFQTVAESLTYGPASIPIVSTVTGGLADEAFGTP
                     QYWVGQVRGAVRFADAVTTLAGQGVTRFVELGPDAVLTAMAQQTLDEAADSAIFTATMR
                     AGRPEPGTVVAALGQLHTAGVPVDWQAFYAETGARRIDLPTYAFQREFYWLEHEQEGVD
                     ATAMGLERVEHPLLGAAIPAPDSDGISFTGRLSVRTHPWLADHQVLDNILFPGTGFVEL
                     AVAAGSQVGCAVLEELTLAAPLLLPAGDPGSRDRGMQVQVVAGAADEADRRTVAVYSRR
                     ENAPADEPWTLHAEGTLTASAPTASTGHLQWPPADATPVDVSDAYDILHERGYGYGPTF
                     QGLKTAWRRGEEIFTEVELPAQAREDAAAFGVHPALLDAALHGLGLVPPDGAADREQAL
                     LPFAWRGVVLHTAGADALRVRLTWLSDTSLSMEITDRYGVPVASVESLTLRATSTEQLR
                     AAEPGAGDSLFQLSWTPIAGAPAAVPVSWAAWADLADDAVPPEVVVLDAGRGRDAAAVR
                     AATHQALAVLQAWAADSRFAGSTLAVTTRGAVPLPGEDPADLAGAAVWGLVRSAQSENL
                     GRILLLDTDDSVDLATVLATDEPQVVVRDGVAHAARLARPPAPAEDGTPAGSFGPDDAV
                     LVTGATGTLGRLVSRHLVTRYGVTRLLLTSRRGLAAPGAAELRDELAALGADVTVAACD
                     TADRAALAGLLSTVDVAGVVHIAGVLDDGVISSLTPERVDHVLRAKVDAALNLHELTAG
                     RNLSAFVLFSSAAGVFGNPGQGSYAAANAFLDALALHRRAAGLTAQSQAWGLWDESGIG
                     GGLTDADRQRLSRTGVEPLTEEQGLALFDLACAGDAATVVPIRLDLAAVAAGNEPPALL
                     RGLVRRPARRGTGSASAAAALRSSLAGLSETERQAALLDIVRTQAALVLGHNGSEAIEP
                     DRAFGELGFDSLSAVEFRNGLNTVTGLRLPATLVFDYPNALVLAAHLDRELATDADGRN
                     GSADTEEQVRRILNAIPLSRLRDAGLLDTLLELGGARAESAESADSAPAESIDEMDAES
                     LINMAFQGLELDDMTQEW"
     aSDomain        582..1451
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_AT.1"
                     /evalue="2.80E-110"
                     /label="F5972_RS36540_PKS_AT.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="481"
                     /protein_start="191"
                     /score="360.3"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGAQRLGMGRELHAAFPVFAEAFDAVVAELDAHLDRPLREV
                     IWGADAETVDQTAYAQAGLFAVEVALFRLLTSWGVRPDYLAGHSVGELAAAHVAGMLTL
                     SDACRLVAARGRLMQALPSGGAMVAVQATEDEVLPLLTDGVDIAAINGPRAVVVSGQEA
                     PVLALAERLAEQGRKTRRLRVSHAFHSHLMEPMLADFAEVAESLTYGSASIPIVSTVTG
                     GLADEAFGTPEYWVGQVRGAVRFADAVTTLAAQGVTRFVELGPDAVLTAMAQQTLDEAA
                     DTAVFTATM"
     aSModule        582..4154
                     /complete
                     /domains="nrpspksdomains_F5972_RS36540_PKS_AT.1"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_DH.1"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_KR.1"
                     /domains="nrpspksdomains_F5972_RS36540_ACP.1"
                     /locus_tags="F5972_RS36540"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       585..632
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0001"
                     /evalue="9.40E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="208"
                     /protein_start="192"
                     /score="15.1"
                     /tool="antismash"
                     /translation="FTGQGAQRLGMGRELH"
     CDS_motif       756..803
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0002"
                     /evalue="3.70E-01"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="265"
                     /protein_start="249"
                     /score="4.6"
                     /tool="antismash"
                     /translation="YAQAGLFAVEVALFRL"
     CDS_motif       816..938
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0003"
                     /evalue="3.00E-23"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="310"
                     /protein_start="269"
                     /score="74.1"
                     /tool="antismash"
                     /translation="GVRPDYLAGHSVGELAAAHVAGMLTLSDACRLVAARGRLMQ"
     CDS_motif       1023..1061
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0004"
                     /evalue="4.70E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="F5972_RS36540"
                     /protein_end="351"
                     /protein_start="338"
                     /score="13.4"
                     /tool="antismash"
                     /translation="AAINGPRAVVVSG"
     CDS_motif       1125..1151
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0005"
                     /evalue="6.50E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="F5972_RS36540"
                     /protein_end="381"
                     /protein_start="372"
                     /score="6.7"
                     /tool="antismash"
                     /translation="LRVSHAFHS"
     CDS_motif       1281..1316
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0006"
                     /evalue="1.30E-02"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="F5972_RS36540"
                     /protein_end="436"
                     /protein_start="424"
                     /score="9.2"
                     /tool="antismash"
                     /translation="YWVGQVRGAVRF"
     aSDomain        1689..2198
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_DH.1"
                     /evalue="2.80E-62"
                     /label="F5972_RS36540_PKS_DH.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="730"
                     /protein_start="560"
                     /score="201.9"
                     /tool="antismash"
                     /translation="HPLLGAVLPLPESGGVVFTGQLSVRSHPWLADHQVFGSVLLPGTG
                     LVELALHAAEHTGCTLVEELTLAAPLVLPGGAAGSGEGAVQVQVVVGAADGDGRRTVAI
                     YSRGAQAPADLPWTLHAEGVLASETAQNGADLSQWPPAEATAVDLTDAYAILNERGYGY
                     GPAFQGL"
     CDS_motif       1773..1850
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0007"
                     /evalue="1.70E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="614"
                     /protein_start="588"
                     /score="33.3"
                     /tool="antismash"
                     /translation="WLADHQVFGSVLLPGTGLVELALHAA"
     CDS_motif       2166..2198
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0008"
                     /evalue="6.70E-02"
                     /label="PKSI-DH_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="730"
                     /protein_start="719"
                     /score="6.6"
                     /tool="antismash"
                     /translation="GYGYGPAFQGL"
     aSDomain        3093..3629
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_KR.1"
                     /evalue="2.40E-59"
                     /label="F5972_RS36540_PKS_KR.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1207"
                     /protein_start="1028"
                     /score="192.3"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGMLGRLVARHLVAAHGVRRLLLTSRSASAESAADLV
                     GELADLGAEVSVAAADAADRASLAGVLAGIPAEHPLVGVVHLAGVLDDGVIASLTPERM
                     DRVLRPKVDAALNLHELTRDHDLAAFVLFSSASGVFGTPGQGSYAAANAFLDALAAHRR
                     AGGLTAQSLAWGLWAD"
     CDS_motif       3096..3197
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0009"
                     /evalue="6.30E-09"
                     /label="PKSI-KR_m1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1063"
                     /protein_start="1029"
                     /score="28.5"
                     /tool="antismash"
                     /translation="TVLVTGGTGMLGRLVARHLVAAHGVRRLLLTSRS"
     CDS_motif       3420..3449
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0010"
                     /evalue="1.50E-01"
                     /label="PKSI-KR_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1147"
                     /protein_start="1137"
                     /score="6.0"
                     /tool="antismash"
                     /translation="PKVDAALNLH"
     CDS_motif       3474..3581
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0011"
                     /evalue="3.10E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1191"
                     /protein_start="1155"
                     /score="64.8"
                     /tool="antismash"
                     /translation="AAFVLFSSASGVFGTPGQGSYAAANAFLDALAAHRR"
     aSDomain        3939..4154
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_ACP.1"
                     /evalue="2.70E-28"
                     /label="F5972_RS36540_ACP.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1382"
                     /protein_start="1310"
                     /score="90.1"
                     /tool="antismash"
                     /translation="LDIVRTQAAAVLGHAGVEAIEPDRAFGELGFDSLSAVELRNGLNA
                     ATGLRLPASLVFDYPNALAVAEHIADE"
     aSDomain        4221..5453
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="3.90E-178"
                     /label="F5972_RS36540_PKS_KS.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1815"
                     /protein_start="1404"
                     /score="584.6"
                     /tool="antismash"
                     /translation="AIVGMACRYPGGVSSPEDLWRLVAGGVDAISDLPVNRGWNIEEIF
                     DPEGKRPNTSYVKSGGFLHEAAEFDAAFFGISPNEALTMDPQQRLLLETSWEAMERAGI
                     DPGSLRGSSTGVFAGMMYHDYAANANTGSIASGRVSYVFGFEGPSVTIDTACSSSLVAL
                     HLAGQALRSGECSLALVGGVAVMATPDTFIEFSRQRGLSPDGRCRAYGAGANGTAWAEG
                     CGVLLVERLSDARRLGHRVLAVVRGTAVNQDGASNGLTAPNGPSQERVIRQALANARLS
                     VSDVDVVEGHGTGTTLGDPIEAQAVLATYGQDRPEEQPLWLGSLKSNIGHAQAAAGVGG
                     IIKMVMAMHNGVLPKTLHADEPSPHVEWSAGHVRLLTEAMPWPEQDRPRRAGVSSFGIS
                     GTNAHVIIEQAP"
     aSModule        4221..9500
                     /complete
                     /domains="nrpspksdomains_F5972_RS36540_PKS_KS.1"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_AT.2"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_DH.2"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_KR.2"
                     /domains="nrpspksdomains_F5972_RS36540_PKS_PP.1"
                     /locus_tags="F5972_RS36540"
                     /monomer_pairings="mal -> ccmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       4659..4706
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0012"
                     /evalue="8.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1566"
                     /protein_start="1550"
                     /score="20.8"
                     /tool="antismash"
                     /translation="GPSVTIDTACSSSLVA"
     CDS_motif       5082..5126
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0013"
                     /evalue="5.90E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1706"
                     /protein_start="1691"
                     /score="9.5"
                     /tool="antismash"
                     /translation="VEGHGTGTTLGDPIE"
     CDS_motif       5187..5231
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0014"
                     /evalue="7.90E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1741"
                     /protein_start="1726"
                     /score="9.2"
                     /tool="antismash"
                     /translation="GSLKSNIGHAQAAAG"
     CDS_motif       5727..5750
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0015"
                     /evalue="2.80E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="F5972_RS36540"
                     /protein_end="1914"
                     /protein_start="1906"
                     /score="-1.2"
                     /tool="antismash"
                     /translation="PVAWVVSG"
     aSDomain        5997..6857
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_AT.2"
                     /evalue="8.70E-108"
                     /label="F5972_RS36540_PKS_AT.2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2283"
                     /protein_start="1996"
                     /score="352.1"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGAQRLGMGRELHAAFPVFADAFDAVAGELDAHLDRPLREV
                     IWGADPVLADQTAYAQAGLFAVEVALFRLVSSWGVRPDYLAGHSVGELAAAHVAGMLTL
                     ADASRLVAARGRLMQALPAGGAMVAVQATEDEVTPLLTDGVDIAAINGPRAVVVSGQEG
                     PVLALAERLAEQGRKTRRLRVSHAFHSHLMEPMLADFQTVAESLTYGPASIPIVSTVTG
                     GLADEAFGTPQYWVGQVRGAVRFADAVTTLAGQGVTRFVELGPDAVLTAMAQQTLDEAA
                     DSAIFT"
     CDS_motif       6000..6047
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0016"
                     /evalue="9.40E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2013"
                     /protein_start="1997"
                     /score="15.1"
                     /tool="antismash"
                     /translation="FTGQGAQRLGMGRELH"
     CDS_motif       6171..6218
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0017"
                     /evalue="4.40E-01"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2070"
                     /protein_start="2054"
                     /score="4.4"
                     /tool="antismash"
                     /translation="YAQAGLFAVEVALFRL"
     CDS_motif       6231..6353
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0018"
                     /evalue="7.10E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2115"
                     /protein_start="2074"
                     /score="69.7"
                     /tool="antismash"
                     /translation="GVRPDYLAGHSVGELAAAHVAGMLTLADASRLVAARGRLMQ"
     CDS_motif       6438..6476
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0019"
                     /evalue="4.70E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2156"
                     /protein_start="2143"
                     /score="13.4"
                     /tool="antismash"
                     /translation="AAINGPRAVVVSG"
     CDS_motif       6540..6566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0020"
                     /evalue="6.50E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2186"
                     /protein_start="2177"
                     /score="6.7"
                     /tool="antismash"
                     /translation="LRVSHAFHS"
     CDS_motif       6696..6731
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0021"
                     /evalue="1.30E-02"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2241"
                     /protein_start="2229"
                     /score="9.2"
                     /tool="antismash"
                     /translation="YWVGQVRGAVRF"
     aSDomain        7077..7586
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_DH.2"
                     /evalue="2.70E-59"
                     /label="F5972_RS36540_PKS_DH.2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2526"
                     /protein_start="2356"
                     /score="192.2"
                     /tool="antismash"
                     /translation="HPLLGAAIPAPDSDGISFTGRLSVRTHPWLADHQVLDNILFPGTG
                     FVELAVAAGSQVGCAVLEELTLAAPLLLPAGDPGSRDRGMQVQVVAGAADEADRRTVAV
                     YSRRENAPADEPWTLHAEGTLTASAPTASTGHLQWPPADATPVDVSDAYDILHERGYGY
                     GPTFQGL"
     CDS_motif       7161..7238
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0022"
                     /evalue="1.80E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2410"
                     /protein_start="2384"
                     /score="33.2"
                     /tool="antismash"
                     /translation="WLADHQVLDNILFPGTGFVELAVAAG"
     CDS_motif       7554..7586
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0023"
                     /evalue="1.40E-01"
                     /label="PKSI-DH_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2526"
                     /protein_start="2515"
                     /score="5.7"
                     /tool="antismash"
                     /translation="GYGYGPTFQGL"
     aSDomain        8457..8981
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_KR.2"
                     /evalue="5.40E-53"
                     /label="F5972_RS36540_PKS_KR.2"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2991"
                     /protein_start="2816"
                     /score="171.6"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="AVLVTGATGTLGRLVSRHLVTRYGVTRLLLTSRRGLAAPGAAELR
                     DELAALGADVTVAACDTADRAALAGLLSTVDVAGVVHIAGVLDDGVISSLTPERVDHVL
                     RAKVDAALNLHELTAGRNLSAFVLFSSAAGVFGNPGQGSYAAANAFLDALALHRRAAGL
                     TAQSQAWGLWDE"
     CDS_motif       8460..8558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0024"
                     /evalue="9.00E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2850"
                     /protein_start="2817"
                     /score="18.4"
                     /tool="antismash"
                     /translation="VLVTGATGTLGRLVSRHLVTRYGVTRLLLTSRR"
     CDS_motif       8772..8801
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0025"
                     /evalue="8.10E-01"
                     /label="PKSI-KR_m3"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2931"
                     /protein_start="2921"
                     /score="3.9"
                     /tool="antismash"
                     /translation="AKVDAALNLH"
     CDS_motif       8829..8933
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS36540_0026"
                     /evalue="3.10E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="F5972_RS36540"
                     /protein_end="2975"
                     /protein_start="2940"
                     /score="64.7"
                     /tool="antismash"
                     /translation="AFVLFSSAAGVFGNPGQGSYAAANAFLDALALHRR"
     aSDomain        9243..9500
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_PP.1"
                     /evalue="3.80E-28"
                     /label="F5972_RS36540_PKS_PP.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="3164"
                     /protein_start="3078"
                     /score="89.7"
                     /tool="antismash"
                     /translation="LAGLSETERQAALLDIVRTQAALVLGHNGSEAIEPDRAFGELGFD
                     SLSAVEFRNGLNTVTGLRLPATLVFDYPNALVLAAHLDREL"
     aSDomain        9537..9728
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS36540_PKS_Docking_Cterm.
                     1"
                     /evalue="1.50E-09"
                     /label="F5972_RS36540_PKS_Docking_Cterm.1"
                     /locus_tag="F5972_RS36540"
                     /protein_end="3240"
                     /protein_start="3176"
                     /score="30.5"
                     /tool="antismash"
                     /translation="TEEQVRRILNAIPLSRLRDAGLLDTLLELGGARAESAESADSAPA
                     ESIDEMDAESLINMAFQGL"
     gene            9764..>10302
                     /locus_tag="F5972_RS36545"
                     /old_locus_tag="F5972_36550"
     CDS             9764..>10302
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082208.1"
                     /locus_tag="F5972_RS36545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_36550"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_150940710.1"
                     /sec_met_domain="mod_KS (E-value: 1e-67, bitscore: 215.9,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 5.3e-49, bitscore:
                     154.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDSDVKLVEALRASLKETERLRGEHRKIIAAQHEPIAIIGMACR
                     YPGGVSSPEDLWRLVSDGVDAISDWPADRGWDVEGLFDPSGERANTSYVKSGGFLHEAA
                     EFDAGFFGISPNEALVMDPQQRLLLETSWEAMERAGIDPGSLRGSSTGVFAGMMYHDYA
                     ANANTGSIASGRVSYV"
ORIGIN
        1 ttcagttgct gaccgaggcg atgccgtggc cggaggtgga ccgtccccgc cgggcgggtg
       61 tgtcgtcgtt cggcatcagc gggaccaacg cccacgtgat catcgagcag gcccccgccg
      121 acctcgctgc cgaggtcact ctcaccgagg cccccgcccc ggccctgctc gtcggcaccg
      181 tcgacccggc cacggccgag gtggccgacg ccaccgccct ggccttggcc gagagcgggc
      241 agcccgccgg tgccgctgta gcgggagagg gtgcgaccca ggccggagcg ccggatacct
      301 cggtggttcc ggtggcgtgg gtgctgtcgg ggaagacgcc ggacgcgttg gccgggcagg
      361 cggcgcgtgt gctgtccttc gtgcaggacc gtcccgccct gagtgtcgcg gatgtcgggt
      421 tgtcgctggc gacgacgcgg gcgtcgttcg agcggcgggc ggtggtcacg ggtgccgacc
      481 gggccgccct gctggcgggg ttggctgcac tggcggaggg tcgtaccgct cctggggcgg
      541 tgcagggcgt cgcccgctcc gccgggaagc tgggtgtgtt gttcaccggt cagggcgccc
      601 agcggctcgg catgggccgc gaactgcatg ccgcgttccc ggtgttcgcc gaagcgttcg
      661 acgccgtcgt cgccgagctg gacgcgcacc tggaccggcc gttgcgtgag gtgatctggg
      721 gcgccgacgc cgagacggtc gaccagaccg cgtacgcgca ggccgggttg ttcgccgtcg
      781 aggtcgcgct gttccgtctc ctgacgtcgt ggggggtgcg gccggactac ctggccgggc
      841 actcggtcgg tgagctggcc gccgcccacg tggcgggcat gctgactctc agcgacgcgt
      901 gccgccttgt ggcggcgcgg ggccggctga tgcaggccct cccgtccggc ggtgcgatgg
      961 tcgcggttca ggccaccgag gacgaggtac tgccgctgct gacggacggg gtggacatcg
     1021 ccgcgatcaa cgggccgcgc gcggtggtgg tctccgggca ggaagccccc gtcctggctt
     1081 tggcggagcg gttggcggag caggggcgga agacgcgccg gttgcgggtc tcccacgcct
     1141 tccactccca cctgatggag ccgatgctcg ccgacttcgc cgaggtggcg gagtccctga
     1201 cctacgggtc ggcgtccatc ccgatcgtgt ccaccgtcac cggcggcctg gccgacgagg
     1261 cgttcggcac cccggagtac tgggtggggc aggtgcgggg cgcggtgcgg ttcgccgacg
     1321 cggtcaccac cctggccgcg cagggcgtga cccggttcgt ggagctgggc ccggacgcgg
     1381 tgctgaccgc gatggcccag cagaccctcg acgaggcagc cgacaccgcg gtcttcaccg
     1441 ccaccatgcg ggccgagcgc cccgagcccg gcaccgtcgt caccgccctg ggtcagctcc
     1501 acaccgccgg cgttccggtc gactggcagg ccttctacgc cgagaccggc gcccgccagg
     1561 tcgacctgcc cacctatgcc ttccagcgcg agcgctactg gctcaacccg atcgactacc
     1621 tgtccgagtc gtgggtggcc gaccacatcg gcaacctgtc ctccgccggg ctggaggccg
     1681 tcgagcaccc gctgctgggt gccgtcctcc cgctgcccga gtccgggggc gttgtcttca
     1741 ccggtcagct gtccgtacgg agtcatccgt ggctggccga tcaccaggtg ttcggcagtg
     1801 tgctgttgcc ggggacgggt ctggtggagc tggcgttgca cgcggccgag cacaccggct
     1861 gcactctggt ggaggagctg acgctggcgg cgccgctggt gctgccgggc ggggcggccg
     1921 ggtccggtga gggcgcggtg caggtgcagg tggtggtcgg cgccgccgac ggcgacggcc
     1981 gccgtacggt ggcgatctac tcgcggggcg cgcaggcccc ggccgacctg ccctggacgc
     2041 tgcacgccga gggcgtcctg gcctccgaga ccgcacagaa tggtgcggac ctgagccagt
     2101 ggcctccggc cgaggccacc gcggtggacc tcaccgacgc ctacgcgatc ctcaacgagc
     2161 gcggctacgg ctacggcccg gccttccagg gcctgcaggc ggcctggcga cgcggcgagg
     2221 aactgtacgc cgaggtcgcc ctgcccgacc aggaacacca ggacgccgcc ctcttcgggc
     2281 tccaccccgc gctgttcgac gcggcactgc acgcgctgca cttcggcggg gacgacacgg
     2341 acggcggccc cacgatgctg ccgttctcct gggccggcgt gtcgctgagc gccgtcggcg
     2401 cgacggcgct gcgcgtccgg ctgacgcccg cggggccgaa cgccttctcc ctggagatcg
     2461 cggacgccga gggagcgccg gtcgccgccg tacggtcgct cacgtcgcgg ccggtcgcgc
     2521 cggaacagct gcagcccgcg cgactgcacg actcgctgta ccgggtcgac tggaccaagg
     2581 tgccggtgcc cccggccgtc tccctgacgt acgccgactg ggcggctctg gacccggccg
     2641 cggcgacggt gccggatgtc atcgtgctcc gggtgccctc cggcgacccg gtcggcgctt
     2701 ccgcggacga ggccgcggcg gtcagggcca cgacccacca ggtgctggcc gcgctgcagg
     2761 aatggacggc cggcgagcgg ttcgccgaca gcaggctgct ggtcgtcacc cggggggcgg
     2821 tcgccccgct gtccggctcg gacgacgtga ccgacctcgc cggcgcggcc gtctgggggc
     2881 tggtgcggac cgcgcagcgg gagcaccccg accggatcgt cctcgccgac ctgcatgaga
     2941 gcgcggacga gagcgtggac gtcggcgcgg tcatggccgt cggcgagccg caggtggcgc
     3001 tccgcgacgg agccgcgtac gcgccccgcc tggtccgcgt gcccgccgcg accactgagg
     3061 ccgagccggt gacgtcgttc cccgccgagg ggacggtgct ggtcacgggt ggcacgggga
     3121 tgctgggccg gttggtggca cggcatctgg tggcggcgca tggggtgcgt cgtctgctgc
     3181 tgaccagcag gagcgcctcg gccgagagcg ccgccgacct ggtcggcgag cttgcggacc
     3241 tcggcgccga ggtgtcggtg gcggcggccg acgccgctga tcgtgcctcg ctcgcgggag
     3301 tgctggcagg catcccggcg gagcatccgc tggtgggagt cgtacacctg gcgggtgtgc
     3361 tggacgacgg tgtgatcgcg tcgctgacgc cggagcgaat ggatcgggtg ctgcgtccga
     3421 aggtggacgc ggcgctgaac ctgcacgagc tgacgcggga tcatgatctg gctgccttcg
     3481 tgctgttctc ctcggcctcg ggagtcttcg ggacgccggg tcagggcagt tacgccgcgg
     3541 cgaacgcgtt cctggacgcg ctggccgcgc accgccgggc gggcggcctc accgcccagt
     3601 cgctggcctg gggactgtgg gccgacgacg ccgggatggc cggcgagctg ggcgacaccg
     3661 accggcagcg gctgagccgc aacggcgtgg agcccctgtc ggctcagcag gggctggagc
     3721 tgttcgacgt cgcgtgcacc gtggactccg gcacgctggt gccgatccgg ctggacttcg
     3781 ccgcgctcgc cgaagcggga gcggagctcc cgtccctctt ccgcacactc gtccgcgtgc
     3841 ccacccgccg ggtggccgcc gcgggcggcg gcgcgtcctc cgccgtacgg gagaggttcg
     3901 ccggactgac cgcggccgag cgggtcgccg ccgctctcga catcgtgcgc acgcaggcgg
     3961 ccgccgtcct cggacatgcc ggggtcgagg cgatcgagcc cgaccgggcc ttcggtgagc
     4021 tgggcttcga ctcgctgtcc gccgtggaac tccgcaacgg actgaacgcg gccaccggac
     4081 tgcggctgcc ggcgtcgctg gtcttcgact accccaacgc gctcgcggtc gccgagcaca
     4141 tcgcggacga ggtggtcggc gccgacaccc ggcgtacggt ggtcgcggcc acggcgggct
     4201 cggttgacga gccgctggcg atcgtgggca tggcgtgccg ctatccgggc ggggtgtcct
     4261 cgccggagga cctgtggcgc ctggtcgcgg gcggggtgga cgcgatctcc gacctaccgg
     4321 tcaaccgcgg ctggaacatc gaggagatct tcgaccccga gggcaagcgg ccgaacacca
     4381 gttatgtgaa gtcgggcggg ttcctgcacg aggcggcgga gttcgacgcg gccttcttcg
     4441 ggatctcccc gaacgaggcg ctgacgatgg acccgcagca gcggctgctg ctggagacct
     4501 cgtgggaggc gatggagcgg gcgggcatcg acccgggctc gctgcgggga tcgtcgaccg
     4561 gggtgttcgc cgggatgatg taccacgact acgccgccaa cgcgaacacc ggatcgatcg
     4621 cctcgggccg cgtgtcgtac gtcttcgggt tcgagggccc gtcggtcacg atcgacaccg
     4681 cctgctcgtc gtcgctggtg gccctgcacc tcgccgggca ggcgctccgg tccggcgagt
     4741 gctcgctggc actggtcggc ggagtcgcgg tgatggccac gcccgacacc ttcatcgagt
     4801 tctcccggca gcgaggcctg tcgcctgatg gtcgctgccg cgcctatggc gcgggggcga
     4861 acggcacggc gtgggccgag ggctgcggcg tgctgctggt cgagcggttg tcggacgcgc
     4921 gccggctcgg ccaccgggtg ctggccgtcg tccggggtac ggcggtgaac caggacggcg
     4981 cctccaacgg tctgaccgcg ccgaacggcc cctcccagga gcgggtgatc cgccaggcgc
     5041 tcgccaacgc ccgcttgtcg gtgagcgacg tggacgtggt cgagggccac ggcaccggga
     5101 ccaccctcgg cgacccgatc gaggcccagg ccgtgctggc cacctacggc caggaccgcc
     5161 cggaggagca gccgctgtgg ctgggatcgc tcaagtccaa catcggtcac gcgcaggcgg
     5221 ccgccggtgt cggcgggatc atcaagatgg tcatggccat gcacaacggc gtcctgccca
     5281 agacgctgca cgccgacgag ccctctccgc acgtggaatg gtcggcgggc cacgtgcgac
     5341 tgctgaccga ggcgatgccg tggccggagc aggaccgtcc gcgccgcgcg ggtgtgtcgt
     5401 cgttcggcat cagcggcacg aacgcccacg tcatcatcga gcaggccccc gccgacctcg
     5461 cggccgacgt ccttgtcgcc gaggctcccg ccccggccgt gctcaccggc accgccctcc
     5521 cggccacggc tgttgccgaa gtcaccgcac tggtgccggg cgatggcgtg tcctccggac
     5581 ccattccggc catggccgag ctcgcggttg ccgccgacgg tgccgcaccg gtcctggctg
     5641 agagcgggca gcccgccggc gccgctgtgg cgggcgaggg cgccgccctg gtcccggcag
     5701 gaagcgcggc ccaggccggt gctccggtgg cgtgggtggt gtcggggaag acggcggacg
     5761 cgttggccgg gcaggcggcg cgggtgctgt cttacgtgca ggaccgtccc tccctcagcc
     5821 ttgcggatgt cgggttctcg ctggcgacga cgcgggcggc gttcgagcgg cgggcggtgg
     5881 tgaccggtgg tgaccgggcc gccctgctgg acgggctggc tgcactggcg gagggccgca
     5941 aggctcctgg ggtggtgcag ggcgtcgccc gatcttcggg gaaggtcggg gtgctgttca
     6001 ccggtcaggg cgcccagcgg ctcggcatgg gccgcgaact ccacgcggcg ttcccggtgt
     6061 tcgccgacgc gttcgacgcc gtcgcaggcg agctggacgc gcacctggac cggccgctgc
     6121 gtgaggtgat ctggggcgcc gaccccgttc tcgccgacca gaccgcgtac gcgcaggccg
     6181 ggttgttcgc cgtcgaggtg gccctgttcc gcctcgtctc ctcgtggggg gtgcggccgg
     6241 actacctggc cggtcactcg gtcggtgagc tggccgccgc ccacgtcgcc gggatgctga
     6301 ctctcgccga cgcgtcccgt ctggtcgcgg cgcggggccg gctgatgcag gccctccccg
     6361 cgggtggggc gatggtcgcg gtccaggcca ccgaagacga ggtgaccccg ctcctgaccg
     6421 acggagtgga catcgccgcc atcaacggcc cccgcgcggt ggtcgtgtcc gggcaggagg
     6481 gcccggtgct ggccctggcg gagcggttgg cggagcaggg gcggaagacg cgccggttgc
     6541 gggtctccca cgctttccac tcccacctga tggagccgat gctcgccgac ttccagaccg
     6601 tggcggagtc gctgacctac gggccggcgt ccatcccgat cgtgtccacc gtcaccggcg
     6661 gcctggccga cgaggcgttc ggcaccccgc agtactgggt gggccaggtg cgcggcgcgg
     6721 tgcggttcgc cgacgccgtg accacgctgg ccgggcaggg tgtgacgcgg ttcgtggagc
     6781 tgggcccgga cgcggtgctg accgcgatgg cccagcagac cctcgacgag gcagccgaca
     6841 gcgcgatctt caccgccacc atgcgggccg gacgccccga gccgggcacc gtcgtcgccg
     6901 ccttgggcca gctgcacacc gccggcgtgc cggtcgactg gcaggccttc tacgccgaga
     6961 ccggcgcccg ccgcatcgac ctgcccacct acgccttcca gcgcgagttc tactggctcg
     7021 aacacgagca ggagggcgtc gacgcgacgg cgatgggcct ggagcgcgtc gagcacccgc
     7081 tgctcggcgc cgcgatcccc gcccccgact cggacgggat ctcgttcacc ggccggctgt
     7141 cggtccgtac gcatccctgg ctggccgacc accaggtgct ggacaacatc ctgttccccg
     7201 gcacgggctt cgtggagctg gccgtcgcgg cgggaagcca ggtcggctgc gccgtcctgg
     7261 aggagctgac cctggccgcg ccgctgctgc tgcccgccgg tgatcccggt tcgcgtgatc
     7321 gcgggatgca ggtgcaggtc gtggccggcg ccgccgacga ggccgaccgg cgtacggtcg
     7381 cggtctactc ccgcagggag aacgctccag cggacgagcc ctggacgctg cacgccgaag
     7441 gcaccctgac cgcttccgcg cccaccgcct ccaccggaca cctgcagtgg cccccggccg
     7501 acgcgacccc ggtggacgtg agcgacgcgt acgacatcct gcacgaacgc ggctacggct
     7561 acggcccgac gttccagggc ctgaagacgg cgtggcggcg cggcgaggag atcttcacgg
     7621 aggtggagct cccggcgcag gcgcgggagg acgcggccgc gttcggcgtg cacccggcgc
     7681 tgctggacgc cgcgctgcac ggtctgggtc tcgtgcctcc ggacggcgcc gccgaccgcg
     7741 agcaggcgct gctgcccttc gcctggcgcg gggtcgtgct gcacacggcc ggagccgacg
     7801 ccctgcgcgt acggctgacc tggctgagcg acacctcgtt gtcgatggag atcaccgacc
     7861 ggtacggcgt gcccgtggcg tcggtcgagt cactgaccct gcgcgcgacg tcgaccgagc
     7921 agcttcgcgc ggcggagccg ggcgcgggcg actcgctctt ccagctgtcc tggacgccga
     7981 tcgccggagc gccggcggcg gtccccgtgt catgggccgc ctgggccgat ctggccgacg
     8041 acgccgtgcc tccggaggtg gtcgtgctgg acgccggccg tggccgcgac gcggcggcgg
     8101 tgcgggccgc gacgcaccag gcgctcgccg tactgcaggc ctgggcggcc gactcccggt
     8161 tcgccggttc cacgctggcc gtgacgacgc ggggagccgt accactgccg ggcgaggacc
     8221 ccgccgatct cgcgggtgcc gcggtctggg gactggtgcg ctccgcgcag tcggagaacc
     8281 tgggccggat cctgctgctg gacaccgacg actcggtcga cctcgcgacc gtcctggcga
     8341 cggacgagcc gcaggtcgtc gtgcgcgacg gcgtggcgca cgccgcgagg ctcgcccggc
     8401 cgccggcccc cgccgaggac ggcacccccg ccgggtcctt cggacctgac gacgccgtcc
     8461 tcgtcaccgg cgcgaccggc acactgggcc ggctcgtctc ccggcacctg gtcacgcgat
     8521 acggcgtgac gcggctgctg ctgaccagcc gccgcggcct cgccgccccg ggggccgccg
     8581 agctacgcga cgaactggcc gcgctgggcg ccgacgtgac ggtcgccgcc tgcgacaccg
     8641 ccgaccgcgc cgcgctcgcc gggctcctgt ccaccgtgga cgtcgccggc gtcgtgcaca
     8701 tcgccggtgt gctcgacgac ggcgtgatct cctcgctcac ccccgagcgc gtggaccacg
     8761 tgctgcgcgc caaggtcgac gccgcgctga acctgcacga actcaccgca ggccggaacc
     8821 tctcggcgtt cgtgctgttc tcctcggccg ccggcgtgtt cggcaacccc ggtcagggca
     8881 gctacgccgc cgcgaacgcc ttcctcgacg cgctggcgct ccaccgccgg gccgccgggc
     8941 tgaccgcgca gtcgcaggcc tggggcctct gggacgagag cggcatcggc ggaggactga
     9001 ccgacgccga ccggcagcgg ctcagccgca ccggtgtgga accgctgacg gaggagcagg
     9061 gcctcgcgct gttcgacctc gcctgcgccg gggacgcggc cacggtggtg ccgatccggc
     9121 tcgacctggc cgcggtcgcg gccggcaacg agccgccggc cctgctgcgc ggcctcgtac
     9181 gccgcccggc ccggcgcggc acgggaagcg cgtcggccgc cgccgccctg cggagcagcc
     9241 tggccggtct gtccgagacc gaacggcagg cggcgctgct ggacatcgtg cgcacccagg
     9301 ccgccctggt gctcgggcac aacggctcgg aggcgatcga gccggaccgg gcgttcggcg
     9361 agctcggctt cgactcgctg tcggccgtcg agttccgcaa cggactgaac acggtcaccg
     9421 ggctgcgcct gccggcgacg ctggtcttcg actacccgaa cgcgctcgtg ctcgccgccc
     9481 acctcgaccg cgaactcgcg accgacgcgg acgggcggaa cggctcggcg gacaccgagg
     9541 aacaggtccg ccggattctg aacgcgattc ccctgagcag gctgcgcgac gcgggcctgc
     9601 tggacaccct gcttgaactc ggcggagccc gcgccgagtc cgccgaatcg gccgattcgg
     9661 cacccgcgga gtccattgac gaaatggacg ccgagagtct catcaacatg gcgttccagg
     9721 gcctggaact cgacgatatg acgcaggaat ggtgaggttg gcgatgactg actccgacgt
     9781 caagctggtc gaggcgctac gggcctcgct gaaggagacg gaacggcttc gcggcgagca
     9841 ccgcaaaatc atcgccgccc agcatgagcc gatcgccatc atcggaatgg cgtgccgtta
     9901 tccgggtggg gtgtcctcgc cggaggattt gtggcggctg gtgtccgatg gtgtggacgc
     9961 gatctcggat tggccggctg atcgtggctg ggatgtggag gggttgttcg atccgagtgg
    10021 ggagcgggcg aacaccagtt atgtgaagtc gggtgggttc ctgcacgagg cggcggagtt
    10081 cgacgcgggg ttcttcggga tctcgccgaa cgaggcgttg gtgatggatc cgcagcagcg
    10141 gttgctgctg gagacctcgt gggaggcgat ggagcgggcc gggatcgacc cggggtcgct
    10201 gcgggggtcg tcgaccgggg tgttcgccgg gatgatgtat cacgactacg ccgccaacgc
    10261 gaacaccggg tcgatcgcct cgggccgggt gtcgtacgtg tt
//
