LOCUS       NZ_VYTZ01000020        22849 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Microbispora cellulosiformans strain Gxj-6 Scaffold20, whole genome
            shotgun sequence.
ACCESSION   NZ_VYTZ01000020
VERSION     NZ_VYTZ01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora cellulosiformans
  ORGANISM  Microbispora cellulosiformans
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 120244)
  AUTHORS   Gong,X.
  TITLE     Screening of Novel Bioactive Compounds from Soil-Associated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 120244)
  AUTHORS   Gong,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) College of Land and Environment, Shenyang
            Agricultural University, No. 120, Dongling Road, Shenhe District,
            Shenyang, Liao Ning 110866, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008728085.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 02:05:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,471
            CDSs (total)                      :: 7,401
            Genes (coding)                    :: 7,312
            CDSs (with protein)               :: 7,312
            Genes (RNA)                       :: 70
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 7 of 89
            Pseudo Genes (incomplete)         :: 80 of 89
            Pseudo Genes (internal stop)      :: 8 of 89
            Pseudo Genes (multiple problems)  :: 6 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:06:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34033
            Orig. end                         :: 56882
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VYTZ01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="1"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22849
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12849](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    complement(1745..1758)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            complement(2488..3231)
                     /locus_tag="F5972_RS33985"
                     /old_locus_tag="F5972_33990"
     CDS             complement(2488..3231)
                     /EC_number="7.6.2.14"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654882.1"
                     /locus_tag="F5972_RS33985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_33990"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_225312143.1"
                     /transl_table=11
                     /translation="MSATITPARETGLAIRLEGVSLGYGRHAVLEDLHLDVAPGEVLVI
                     VGPSGSGKSTILRAVAGLLTPRAGRVLAQGVPVAGPSPDRAMVFQDDALLPWRTVRRNV
                     ELPLRIRGVPRRERGPAAESWIARVGLAGWEDRLPRELSGGMRQRVQLARSLAAAPRAV
                     LMDEPFGALDAQTRGEMQRLLLDVLRDTAATVVFVTHDVDEALLLADRVVVLGRGGIGR
                     VLPVPGPRTPETDRAGLRARIIEEL"
     gene            complement(3228..4196)
                     /locus_tag="F5972_RS33990"
                     /old_locus_tag="F5972_33995"
     CDS             complement(3228..4196)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630144.1"
                     /locus_tag="F5972_RS33990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_33995"
                     /product="ABC transporter permease"
                     /protein_id="WP_225312144.1"
                     /transl_table=11
                     /translation="MSTPAPAASLAANSPTAEPPAAESPGSEPLASGAVPAARPGRRVR
                     GRATTAGLRRWAVRLGSLVVVVALWQWLTTADVRAGLRFDRLPSPAEVAAEFSRQLTGG
                     LYYLDLAQSMIRILTGFALASVAGIALGVLVARSRWAADVLLPPIEVVRPIPAIALVPI
                     AILVFPTDEQGIVFITFAAAFFPIMVSTRHAVRALPTLWEDAVRTMGGGRAHVLWHVVL
                     PGTLPGVFGGLSVGMGVAWICVISAEMISGEFGVGYRTWHAYTVVDYPAVLVGMASIGV
                     LGWLTSASIELLGRRLTRWLPRAEEGPGERGAGQGAEEGAR"
     gene            complement(4193..5620)
                     /locus_tag="F5972_RS33995"
                     /old_locus_tag="F5972_34000"
     CDS             complement(4193..5620)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654884.1"
                     /locus_tag="F5972_RS33995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34000"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_150939776.1"
                     /transl_table=11
                     /translation="MRKIFATVALLAAAGGALSGCSVSAGADDGKETVVVGYQSKTINT
                     VTAGTLLRGLGYFEKRLGGRYRVEWQDYDTGAPITAKMVAGKIDIGSMGDYPLLINASR
                     TQGVSAARTRLVSVTGYNLRGGLNSVVVAPSSKARTLADLKDAKVSASSGSAGHGTLVQ
                     ALERAGLDPATDVEVVNQQPPVGASALQSGNVQGYAQFVAWPGLLVFKNQARLVYDGSA
                     LDVPTFHGVVVREAYAKERPEVLQAFLQAQIDATKYLHEHPVEAAEAVAKATGLPPEVV
                     YLYNGRDGIATFDVTLKSRLLAALDHDQKYLKSIGTDFTGVDVAKFADDSYIRKAYGAS
                     YDADATGTVNAARISGTDAACGTTAGDPAKAGELWLTGEDTLRPAATPTCLLRQIAAAT
                     SGGGSVRAAYVPDAATGTHWFADRSVWVRDPAAEENERFKPFTTRDGAEAFVKEHARAA
                     VVGYDEALKAAAGGASR"
     gene            complement(5635..5862)
                     /locus_tag="F5972_RS34000"
                     /old_locus_tag="F5972_34005"
     CDS             complement(5635..5862)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453436.1"
                     /locus_tag="F5972_RS34000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34005"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_079312946.1"
                     /transl_table=11
                     /translation="MTLVNSRVDVPVTIDESLCIEGCTLCVDVCPLDSLAIHEVSGKAY
                     MHVDECWYCGPCADRCPADAVTVNIPYLLR"
     gene            complement(5846..6595)
                     /locus_tag="F5972_RS34005"
                     /old_locus_tag="F5972_34010"
     CDS             complement(5846..6595)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630141.1"
                     /locus_tag="F5972_RS34005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34010"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_150939779.1"
                     /transl_table=11
                     /translation="MELADVRRRRADRARQVADLLRCEIVRGGFDGGHLPLEAALAREF
                     GTSRNTVRDALDLLRDEGLIARCPGVGTTVAAEKYPHGLYRLLGLAETLREHGEVTNEV
                     RAMGLVPPPAAVRQRLDLPEGEPVVYIERLRRLDGLPLSLDLTYLVRELGEPLFDADLV
                     HNDVFVLLERIAGRPLGAAELTIEAVNADAHTAAVLDAPRGAALLMVERLTHLSDGRPV
                     DLEFVRFRGDRLTMRGHLWRNTDDPGQ"
     gene            6899..7144
                     /locus_tag="F5972_RS34010"
                     /old_locus_tag="F5972_34015"
     CDS             6899..7144
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F5972_RS34010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="F5972_34015"
                     /product="hypothetical protein"
                     /protein_id="WP_191910452.1"
                     /transl_table=11
                     /translation="MSTTQTRAPRNGVGTPTLFATLNAVKGDPEPAKFRFRATNTWISG
                     THNRSAIRGSPCGSPATRPPTCCTGWSSSPGRFGGL"
     gene            complement(7333..8580)
                     /locus_tag="F5972_RS34015"
                     /old_locus_tag="F5972_34020"
     CDS             complement(7333..8580)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888683.1"
                     /locus_tag="F5972_RS34015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34020"
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="WP_150939781.1"
                     /transl_table=11
                     /translation="MRVLLVGAGGVGTAITRIAARRSFFEHMVVADYDLSRAEAAVAAL
                     GEAGARFSACRADAGDEAAVAALLAEHRCDVLLNATDPRFVMPLFRAALTAGAHYLDMA
                     MSLSRPHPDRPHEECGVKLGDAQFETAAEWERAGRLALVGMGVEPGLSDVFARYAADEL
                     FDHIEEIGVRDGADLTVDGYDFAPSFSIWTTIEECLNPPVVYENGRGWFTTPPFSEPEV
                     FDFPEGIGPVECVNVEHEEVLLMPRWVDTERVTFKYGLGEEFIQTLRTLHLLGLDRTEP
                     VRVAGADGVPVQVSPRDVVAACLPDPATLGERMHGRTCAGTWVRGTKDGGPREVYLYHV
                     VDNQWSMREYGSQAVVWQTAVNPVVALELMASGAWSGAGVLGPEAFPPRPFLDLLTAYG
                     TPWGMREQSASQPSRA"
     misc_feature    complement(8585..8598)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    8594..8607
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.1"
                     /tool="antismash"
     misc_feature    complement(8595..8608)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.13"
                     /tool="antismash"
     gene            8694..9425
                     /locus_tag="F5972_RS34020"
                     /old_locus_tag="F5972_34025"
     CDS             8694..9425
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541262.1"
                     /locus_tag="F5972_RS34020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34025"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_150939783.1"
                     /transl_table=11
                     /translation="MPKPVVGESARRRRRPTKSGVVLSEGLIVRTALRMLREHGPAGLT
                     VRRLGIALGADPSTLYRYFRGVDDLTLAIGDLLIGRAMDGWRGGGDWRAGLRDLGLRIH
                     AAYLEHPQAAVLTASRISGKAHEIAADEAILGLLRGAGFPDHEAVGVYHTFIDMTLAFA
                     ALDAASLALPAEAREADEEIWRATYARLPRATHPNIAAVSPLLVAHMNDSAYPAALEML
                     LDSAASRLTRPVSPPAEPGRA"
     misc_feature    9772..9786
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.73"
                     /tool="antismash"
     gene            9903..10001
                     /gene="mftA"
                     /locus_tag="F5972_RS34025"
                     /old_locus_tag="F5972_34030"
     CDS             9903..10001
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049342.1"
                     /locus_tag="F5972_RS34025"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="F5972_34030"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_117409937.1"
                     /transl_table=11
                     /translation="MEETTVPAEATEELTAETLIEEVSIDGMCGVY"
     gene            10001..11626
                     /gene="mftC"
                     /locus_tag="F5972_RS34030"
                     /old_locus_tag="F5972_34035"
     CDS             10001..11626
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03962.1"
                     /locus_tag="F5972_RS34030"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="F5972_34035"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_150939785.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 3.2e-29,
                     bitscore: 93.3, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4.3e-24, bitscore: 78.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.6e-10, bitscore: 33.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 4e-15, bitscore: 48.3,
                     seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDPLDEAWELSPRVALRPEPFGALAYHFGNRRLTFLKRPELVSV
                     LRDLSGEPDARAALTRAGVPAGRWPAYAEALRGLAGADMIRPRGRTEADRTRPPHVTAA
                     PGTAGQSTTAQSLPAKNAAARSGTASGSLAKRFEYGLAAPICLTWELTYACNLSCVHCL
                     SSSGRRDPRELATAQCEAVIDELQAMQVFYVNIGGGEPTVRPDFWHLVQYAVNHQVGVK
                     FSTNGARLTPERARFLAATDYVDVQVSLDGATPEVNDRVRGPGSYETALRALDNLAAAG
                     FADAKLSVVVTRENAGQLDEFKAIADERGATLRLTRLRPSGRGADVWDELHPTAAQQRD
                     LYEWLLRAGEDVLTGDSFFHLAGYGESLPGLNLCGAGRVVCLIDPLGDVYACPFAIHDR
                     FRAGNVLSDGGFAAVWRESELFGDLRSPQTSGACTGCGFFDSCRGGCMAAKFFTGLPLD
                     GPDPECVQGHAAAALAAGDRTVPRPSQDHSRRPGARRAGAGPVPVRIGRRPSPAAPPVH
                     PPVHPPVHACAESPLAGFTPSSDV"
     aSDomain        10016..10255
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_F5972_RS34030_Mycofactocin_RRE.1"
                     /evalue="1.20E-29"
                     /identifier="RREFam016.1"
                     /label="F5972_RS34030"
                     /locus_tag="F5972_RS34030"
                     /protein_end="85"
                     /protein_start="5"
                     /score="93.3"
                     /tool="antismash"
                     /translation="DEAWELSPRVALRPEPFGALAYHFGNRRLTFLKRPELVSVLRDLS
                     GEPDARAALTRAGVPAGRWPAYAEALRGLAGADMI"
     gene            11644..12849
                     /gene="mftD"
                     /locus_tag="F5972_RS34035"
                     /old_locus_tag="F5972_34040"
     CDS             11644..12849
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658591.1"
                     /locus_tag="F5972_RS34035"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="F5972_34040"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_150939787.1"
                     /sec_met_domain="TIGR03966 (E-value: 2.1e-213, bitscore:
                     700.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAANAWFETVAEAQRRARRHLPKSVYSALLAGSERGRTYHDNTAA
                     FAELGFAPHVADLPAKRDLATTVMGQQISLPVLISPTGVQAVHPDGEVAMARAAAARGT
                     IMGLSNFAGKPVEEVVAANPQTFFQMYWMGTRETMAGHMERARDAGVKGLIVTLDWTFS
                     HGRDWGSPWIPDRLDLKALARFAPQGLTRPRWLLGFARTGRLPDLTVPNVAEPGRPAPT
                     FFGAYGEWMQTPPPSWEDVRWMREQWDGPFMLKGVSRLDDARRAVDAGATAISVSNHGG
                     NNLDGTPATIRLLPGIARVVGDQAEVLLDGGVRRGGDVVKALALGARAVMIGRASLWGL
                     AANGQAGVENVLDILRNGIDSALLGLGRASIHDLEPGDVLVPPGFTREIGVTGEIGSPR
                     DA"
     gene            12842..13600
                     /gene="mftE"
                     /locus_tag="F5972_RS34040"
                     /old_locus_tag="F5972_34045"
     CDS             12842..13600
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852709.1"
                     /locus_tag="F5972_RS34040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34045"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_150939789.1"
                     /transl_table=11
                     /translation="MRERGDGEGGLADLSWPALPPSPTVLVPLGSTEQHGPHLPLHTDT
                     AVAAAVARAAAARLLAAAPDDPVLVAPAVAYGASGEHQDFPGTMSIGTAALRFLIVELV
                     RSLSRWAGRVVIVNGHGGNLDALAAATSLLREEGRDVCWAPCAAGPADAHAGHTETSLV
                     LHLAPDLVDMTRAEPGNLAPLAEIMPALAARGVAAVSPSGVLGDPTRATAGEGRRLLDL
                     MAGDVAARIRHGRADHTGRLLPAPATVSPT"
     gene            13597..14430
                     /locus_tag="F5972_RS34045"
                     /old_locus_tag="F5972_34050"
     CDS             13597..14430
                     /NRPS_PKS="Domain: PKS_KR (15-193). E-value: 3e-09. Score:
                     29.0. Matches aSDomain:
                     nrpspksdomains_F5972_RS34045_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR04504.1"
                     /locus_tag="F5972_RS34045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34050"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_150939791.1"
                     /transl_table=11
                     /translation="MSQVSGGSGPEEGRRVALVTGAARGIGAAVVRALCARGYQVVAVD
                     SCAADGPARPAGVTYPLAADADLKSLADELPGAVVPYIADVRDRDALARAAAVAADRFG
                     RLDAAVAAAAVMAGGLPLWETPDTHVRALWETDVLGVWNTATACVPHMLAGPEPGACRF
                     VAVASAAGSHGLFRLSAYTMAKHAVVGLVRGLAADLVGTGVTAAAVSPGSTRTRMLAAT
                     AEVYGLDGVEGFASSQLLRRLIEPAEIAETIAFCCSREGGVVNGSVISADGGFTP"
     aSDomain        13642..14175
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F5972_RS34045_PKS_KR.1"
                     /evalue="3.00E-09"
                     /label="F5972_RS34045_PKS_KR.1"
                     /locus_tag="F5972_RS34045"
                     /protein_end="193"
                     /protein_start="15"
                     /score="29.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGAARGIGAAVVRALCARGYQVVAVDSCAADGPARPAGVTY
                     PLAADADLKSLADELPGAVVPYIADVRDRDALARAAAVAADRFGRLDAAVAAAAVMAGG
                     LPLWETPDTHVRALWETDVLGVWNTATACVPHMLAGPEPGACRFVAVASAAGSHGLFRL
                     SAYTMAKHAVVGLVR"
     CDS_motif       13648..13710
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F5972_RS34045_0001"
                     /evalue="1.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="F5972_RS34045"
                     /protein_end="38"
                     /protein_start="17"
                     /score="14.3"
                     /tool="antismash"
                     /translation="LVTGAARGIGAAVVRALCARG"
     gene            14427..16067
                     /gene="mftF"
                     /locus_tag="F5972_RS34050"
                     /old_locus_tag="F5972_34055"
     CDS             14427..16067
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="F5972_RS34050"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34055"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_150939793.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 7.2e-21,
                     bitscore: 67.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTNRTPRNRTPRNQTPRNQTSRDRTTGEESAGKQVHGGPAPSD
                     PALSDPALNDRADGGLGDEEKSGGTDPRAAGRLPDGFTVRLNRRVRVRDGGRTLVGGAP
                     TRVMYLTKAAADLFDGRRLRVRGRVSALLADRLLEAGLADPVVGDLPALDPSQVTVVVP
                     VRDRPAALDRLLSGIGGRHRVIVVDDASAAPEAVAEVAAAHGAVLVTLAENAGPAAARN
                     AGLRRVTTPYVAFADSDVVLEPGTVPTLLRHFADPRVAVAAPRVLGLRLPGRDRWILRY
                     EDARSSLDLGLDPGIVRPRAPVSWVPSAFLLARADALGHGFSAEMRVGEDVDLVWRLAR
                     AGWRVRYEPAATVRHEHRTVVHDWLRRKAFYGTGADLLARRHGRDVAPAVLAPWSAAFV
                     LALLAQRRWSLPAAALIFAGTAATMSAKVRRSEHPVRLALTLTSDGAVAAVWQATALLL
                     RHWWPLAAVGCLLSKRLRRAVAAVAVMDALTDLRRTGAHLDPVRYAVARRLDDAAYGSG
                     LWLGAIRGRSVRALLPDIRWKTRSSRPSR"
     gene            complement(16134..16361)
                     /locus_tag="F5972_RS34055"
                     /old_locus_tag="F5972_34060"
     CDS             complement(16134..16361)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025192.5"
                     /locus_tag="F5972_RS34055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34060"
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="WP_150939795.1"
                     /transl_table=11
                     /translation="MGGEELRVVAGNPTEEEICALVCALYAVAADRGDGGEPGPVERPK
                     RHRPRLRLPDYGSPRSWVACHRTDALTGVR"
     gene            complement(16361..17950)
                     /locus_tag="F5972_RS34060"
                     /old_locus_tag="F5972_34065"
     CDS             complement(16361..17950)
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853101.1"
                     /locus_tag="F5972_RS34060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34065"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_225312145.1"
                     /transl_table=11
                     /translation="MGTVVTRVSDLPKESMRERLADLQVLKESIHSGPSEEATARQHAK
                     GKLTVRERLDVLLDSGSFVEVEAFRQHRATGFGLEHRRPYTDGVVAGWGKVYGRDVMVF
                     AHDFRMFGGALGEAHAEKIHKVMDLAEAAGVPLIGLNDGAGARIQEGVTALAGYGGIFR
                     RNVRMSGVIPQINVMLGPCAGGAAYSPALSDFVFMVQNTAQMFITGPDVVKAVTGEEIS
                     ADDLGGARVHGSVSGVAGFVCPDEVSCLEEVRYLLSLLPANNQELPPSITCTDPANRRN
                     DALLDLVPADPAISYDMSAVIEEIVDDGEFFEFHAGWAQNLICGLARFDGHVTGIVANQ
                     PAHLAGVLDIKASEKGARFIQMCDAFNIPLVSLVDVPGFLPGTDQEHGGIIRHGAKLLY
                     AYCAATVPRISMVLRKAYGGAYIVMDSRSIGADLSFAWPTNEIAVMGAEGAANVIFRRE
                     IAAADDPVEAHARRVEEYRRQLMHPYYAAERGLVDDVIDPGETRATLTRALGLLRAKQA
                     SEPRRKHGNPPM"
     misc_feature    complement(18140..18155)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            complement(18162..20948)
                     /locus_tag="F5972_RS34065"
                     /old_locus_tag="F5972_34070"
     CDS             complement(18162..20948)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890458.1"
                     /locus_tag="F5972_RS34065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_34070"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_150939797.1"
                     /transl_table=11
                     /translation="MNLIERDEALASLQEMLAAALTGKGKVAMLSGTVATGKSALLDAF
                     AEQAVELGALPVAATASRMEQDLPLGVLGQLIEEAPLVEEERVRALALLEEGKRTAMSS
                     GLASETLDQVDAQIAHALCTVLLELSERYPLVIVVDDVHYADRPSLLCLAYLARRVRFA
                     RIMVIFSYSDYVRSTDTFFWTDLLRQPYCGVVQLNPLSRQGVHTLVRERAGEEVAVRFA
                     EDWYAYSAGNPLLAAALIEDYLGGPSARERDGEPAADEPVVGERYGQAVLTCLHRGEPE
                     LLRIARCLAVLGETDSLDRLLGLGHTQISRAMNALTAGGVLSVGRFRHESARTAVLAEV
                     GLDERRDLHRRAAGLAHADGAATSVIADHLVQAGPIDAPWVVPVLEDAARDALREGRTE
                     RGVDYLRLAWQACTDERRRLKIATMLVRAEWRINPEAPDRHLADLTEAMQRGHLRGGDG
                     VVLARALLWHGRYDDAQDVIEHLTTAGYESDPETVMELNIARPWLRATHPAFASKLGEP
                     RADTGTALVSVAAGRRLETATALAQVLVNGPHEGALIAVERILRLARLDEMSMDTVECA
                     LLALTYGGQAEKAAPLCDLFSVEASSRRAPSRQARLSAIRAEMAVRQGDMVSAEQYARA
                     ALEIMPITSWGVTVGSPLGSLVIALTAMGRFDDVHDILDRPVLDAMFESRYGLHYLYAR
                     GRYSLAVGQAALALRDFQLCGELTGRWGLDVPGLLPWRAGAAEACLQLGRTEQAAKLIE
                     DQLSRCNSRTPRELGMTIRLLAATSELRHRPMLLRKSADMLQNGGDRYEQARSLFDLAE
                     AYRGLGEHRRVGMIMIRARALMKECGAEIGGPQAAAETGKQTAPALSGGSAALLSEAER
                     RVAVLAAAGHTNREIAGELYLTVSTVEQHLTRIYRKLDITSRADLPAVLEFGGAAVG"
     misc_feature    complement(20910..20912)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(21007..21021)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
     misc_feature    22268..22283
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.68"
                     /tool="antismash"
ORIGIN
        1 tgggtccaag ccggccccgg tgcccatgcc gaactcggtg ccggagccga actcggtgcc
       61 ggagccgggc ccgggctcgc ggtagagagc cgggccgagc ccggcggacg cctccagcgc
      121 ggccgcccgg acgagcggat cggggtccgc ggcgagtcgc acgacagccg ggacgccgtc
      181 gggcaggccg atggtcccga gccctctggc cgcccagacg cgcacctcgc ggtcctcgtc
      241 cccggcggcg agcgccaccc gcccggcgtc gtcgagcgag accagccccc ggaccgcctc
      301 gatccgcacc cgggagtcgg ggtcggcgag cgccgtcccg aacaggtcgg cgtcaccgag
      361 gcgcagcgcc cgcaggacgt caaggacggc cgcgcggacg atcgggtccc cggccgccgc
      421 gaccgcgcgg agggaggcgc cgaaggcggg ggtcgcgggc agcacctcga cgagttcccg
      481 caggcccgtg gcggcggccc gccgtacggt gccgtgcggg tcggcgaggg cctgggccag
      541 cgcggtctcc acgcccgggg gcgtggtctc ggtcagcgtg gcgacggccg ccctgcggac
      601 cttcggttcg gcctccgcca ggtagggggt gatctggtcg agcgacggct cgccgtcggc
      661 gagccgcagc agttcgagga tgcggggcga gcgcccgatc gcctccaccg cgagccgccc
      721 gcccccgcca cgccgggccg cggggccggg cgcgccgtac gcgccgacga cgaccggctc
      781 ggccgggcgg ggcgtgaagc cctcgacggg cacgaggtac ggctcgaccg ggcgcttgac
      841 gaactccatc gccccggcgg cgttcctgcg caggttgagg tgatagagcc actcgtcgtc
      901 gttccgcccg ggcaggtcgg cgcggtcgtg gtagaggccc cacctgctct cggtgcggac
      961 cagggaggac cgggcggcca tctcggcgca gtccctgatg aacgtcacct ccgcgcaccg
     1021 catcagctcg tgcggggtcc gcgcgcccat ggccgcgatc tcgtcgcgca tccgctcgaa
     1081 cgtctccagc gcgaggccga gtttcctgcc ggtcttcggc ggggcgacgt agtcgttgac
     1141 gaacctccgc agcttgtact cgacctgcgg ctgcggcggg ccgtcggggt tgcgcagcgg
     1201 gcggtagatc agctcgtgcg cggcggtgat ctggtcctcc ggcaggccgc cggccgtacg
     1261 gtggtgctcg gcggcgtggg ccccggcgag gtcgccgaag acgaacgcgc cgatcatgta
     1321 gttgtgcggc acgcaggcca ggtcgcccgc ggcgtacagg ccggggacgg tggtcgcgcc
     1381 gttctcgtcc acccagacgc ccgaggccga gtgcccgccg cacaggccga tctcggagat
     1441 gtgcatctcc acgtcgtgcg tgcggtagtc gtgcccgcgc ccggcgtgga aggtgcccct
     1501 ggttggccgc tcggtggtgt gcaggatgtt ctcgagcgcg gtgagcgtct cgtccggcag
     1561 gtgggtggtc ttgaggtaga tcgggccgcg cgccgaggcg atctctccgg cgacctcggc
     1621 catcatctgg cccgaccagt agtcgcagtc gacgaaccgc tccccctcgg cgttgacctg
     1681 atagcccccg aacgggttgg ccacgtaggc gcaggccggg ccgttgtagt ccttaatgag
     1741 cgggttgatc tggaagcact cgatgccgct gagctcggcg cccgcgtggt aggccatcgc
     1801 gtacccgtcc ccggcgttgg tcgggttctc gtacgtgccg tacaggtagc cgctggcggg
     1861 caggcccagc cgcccgcacg ggccggtcgc gagaatcacg gctccggcgg agaccgtgac
     1921 gaaccggccg gaacgggtgt cgaacccggc gaccccgacg gcccggccct cgctcgtgag
     1981 cacgcggacc ggcatcaccc ggttctcgat gcgcaccttc tcgcggatgt cgcggcggcg
     2041 cagcacccgg tagaggacct tcttgacgtc cttgccctcc ggcatcggga ggacgtagct
     2101 gcccgaccgg tggacccggc gcaccgcgta gtcgccgtgc tcgttcttct cgaacttcac
     2161 gccgtagcgc tccagccgcc gcaccatgtc gtagccgcgg gtcgccgtct ggtggacggt
     2221 ccgctggttg accaccccgt cgttggccct ggtgatctcg gcgacgtagt cctcgggcgt
     2281 cgccttgccg gggatgaccg cgttgttcac gccgtccatg cccatcgcga gggcgccgct
     2341 gtgccgtacg tgcgcctttt ccagcaggat cacgtcggcg ccgcgctcgg cggcggtgat
     2401 cgccgccatg gtcccggcgg tgccgccgcc caccaccaga acctggcact ccagatggag
     2461 gcggtctgcc ggggaaggga tgtccatcta aagctcctcg atgatccggg cgcgcagccc
     2521 ggcacggtcg gtctcaggag tgcgcgggcc gggcacgggc aggacgcgtc cgatgccgcc
     2581 gcgcccgagc acgacgaccc ggtcggccag cagcagggcc tcgtccacgt cgtgggtgac
     2641 gaagacgacc gtggcggccg tgtcgcgcag cacgtccagc agcaggcgct gcatctcgcc
     2701 ccgggtctgc gcgtccagcg cgccgaaggg ctcgtccatg agcacggcgc gcggcgcggc
     2761 ggccagggac ctggcgagct gcacgcgctg ccgcatgccg ccggacagct cccggggcag
     2821 ccggtcctcc cagccggcga ggccgacgcg ggcgatccag ctctcggcgg ccggcccgcg
     2881 ctcccgcctc ggcacgccgc ggatgcgcag cggcagctcg acgttccgcc ggaccgtgcg
     2941 ccagggcagc agcgcgtcgt cctggaagac catcgcgcgg tcgggcgacg gcccggcgac
     3001 cgggacgccc tgggccagga cccggccggc gcgcggggtc agcagcccgg cgacggcccg
     3061 caggatcgtg gacttgcccg aaccggacgg tccgacgatc accaggacct cgccgggggc
     3121 gacgtcgagg tgcagatcct ccagtacggc gtgccgcccg tagccgaggg acacaccctc
     3181 cagccggatg gcgagccccg tctcccgggc aggcgtgatc gtcgcgctca ccgggcgccc
     3241 tcctcggcac cctggccggc tccccgctcg ccgggccctt cctccgctct gggcagccat
     3301 cgggtgaggc gccggccgag cagttcgatc gaggcggagg tgagccaccc gagcaccccg
     3361 atcgacgcca taccgacgag caccgccgga tagtcgacca ccgtgtacgc gtgccaggtc
     3421 cggtagccga cgccgaactc gccggagatc atctcggcgg agatcacaca gatccaggcg
     3481 acgcccatgc cgaccgacag cccgccgaag acgcccggca gcgtgccggg gagcacgacg
     3541 tgccacagca cgtgcgcgcg cccgccgccc atggtgcgga ccgcgtcctc ccacagcgtc
     3601 ggcagtgccc gtacggcgtg ccgcgtgctc accatgatcg ggaagaacgc cgcggcgaac
     3661 gtgatgaaca cgatgccctg ctcgtcggtg gggaacacca ggatcgcgat cgggacgagg
     3721 gcgatcgccg ggatcggccg gacgacctcg ataggcggca gcagcacgtc ggccgcccac
     3781 cgggaccgcg cgaccagcac gcccagggcg atgccggcca ccgaggccag cgcgaacccg
     3841 gtgaggatgc ggatcatgct ctgggccagg tcgaggtagt agagcccacc ggtgagctgc
     3901 cggctgaact ccgccgcgac ctcggccggc gagggcaggc ggtcgaaccg cagcccggcg
     3961 cggacgtcgg cggtggtcag ccactgccac agcgcgacga cgaccaccag ggacccaagc
     4021 cgcaccgccc atctgcgcag cccggccgtg gtcgcccggc cgcgtacgcg ccgtcccggc
     4081 cgcgcagccg ggacggcacc ggaggccaaa ggctccgagc ccggtgactc cgcggccggg
     4141 ggctccgcgg tcggggagtt ggcggcgagg gaggcggccg gcgccggggt gctcaccgcg
     4201 acgcacctcc cgccgcggcc ttcagcgcct cgtcgtagcc gacgaccgcc gcccgggcat
     4261 gctccttcac gaacgcctcc gcgccgtcgc gggtggtgaa gggcttgaac ctctcgttct
     4321 cctccgcggc ggggtcgcgc acccagacgg accggtccgc gaaccagtgc gtgcccgtgg
     4381 ccgcgtcggg cacgtacgcc gcccgtacgg acccgccgcc cgaggtggcg gcggcgatct
     4441 gccgcagcag gcaggtcggc gtggccgcgg gacggagcgt gtcctccccc gtcagccaca
     4501 gctcgcccgc cttcgcgggg tcgccggccg tggttccgca ggcggcgtcg gtcccgctga
     4561 tgcgcgccgc gttgaccgtg cccgtggcgt cggcgtcgta ggaggcgccg tacgccttcc
     4621 tgatgtagga gtcgtcggcg aacttcgcca cgtcgacgcc ggtgaagtcg gtgccgatgg
     4681 acttcaggta cttctggtcg tggtccagcg cggccaggag ccgggacttc agcgtgacgt
     4741 cgaaggtggc gatgccgtcg cgcccgttgt agaggtagac gacctcgggc gggagcccgg
     4801 tcgccttggc gaccgcctcg gcggcctcga cggggtgctc gtggagatac ttcgtcgcgt
     4861 cgatctgcgc ctgcaggaac gcctggagca cctcgggccg ctccttcgcg tacgcctccc
     4921 gcaccacgac gccgtggaac gtcggcacgt cgagcgccga cccgtcgtag acgagcctgg
     4981 cctggttctt gaagacgagc agccccggcc acgccacgaa ctgggcgtat ccctgcacgt
     5041 tgcccgactg cagcgcggat gcgcccaccg gcggctgctg gttcacgacc tccacgtcgg
     5101 tggcggggtc gagcccggcc cgctccagcg cctggacgag cgtgccgtgg cccgccgatc
     5161 cggagctggc ggacaccttc gcgtccttca ggtcggccag cgtgcgcgcc ttcgacgacg
     5221 gggcgacgac caccgagttc agcccgccgc gcaggttgta accggtcacc gacacgagcc
     5281 gggtgcgcgc ggcggacacg ccctgggtgc gggaggcgtt gatgaggagc gggtagtcgc
     5341 ccatcgaccc gatgtcgatc ttgccggcca ccatcttcgc ggtgatcggg gctccggtgt
     5401 cgtagtcctg ccactcgacc cggtacctgc cgccgaggcg cttctcgaag tagcccaggc
     5461 cgcggagcag cgtcccggcc gtcaccgtgt tgatggtctt cgactggtat ccgacgacca
     5521 cggtctcctt gccgtcgtcg gcgcccgcgg agacggagca gccggagagg gcgccgccgg
     5581 ccgcggccag cagcgccacg gtggcgaaga tcttgcgcat ggggggatgt ccttctatcg
     5641 gagcaggtag gggatgttca ccgtgacggc gtcggcgggg caccggtcgg cgcaggggcc
     5701 gcagtaccag cactcgtcca cgtgcatgta ggccttgccg ctgacctcgt ggatggccag
     5761 cgagtcgagc gggcagacgt ccacgcacag cgtgcagccc tcgatgcaca gcgactcgtc
     5821 gatcgtcacg ggcacgtcga cgcggctatt gaccagggtc atcggtgttc ctccagagat
     5881 ggccacgcat ggtgagacgg tcgccgcgga accggacgaa ctccaggtcc accgggcggc
     5941 cgtcggagag gtgggtgagg cgctcgacca tcagcagcgc cgctccgcgc ggcgcgtcga
     6001 ggacggcggc ggtgtgcgcg tcggcgttga cggcctcgat cgtgagctcc gccgcgccga
     6061 gcggccgtcc ggcgatccgc tccaggagca cgaacacgtc gttgtgcacg aggtcggcgt
     6121 cgaagagcgg ctcgccgagc tcgcgtacga gataggtcag gtcgagcgag agcggcagcc
     6181 cgtcgagccg gcgcagccgc tcgatgtaga cgacgggctc gccctcgggt aggtcgagcc
     6241 gttgccggac cgcggcgggg ggcggcacga ggcccatggc gcggacctcg ttcgtcacct
     6301 cgccgtgctc ccggagcgtc tcggccagcc cgagcagccg gtagaggcca tgcgggtact
     6361 tctccgccgc gaccgtggtg cccactcccg ggcaccgcgc gatgaggccc tcgtccctga
     6421 gcaggtcgag ggcgtctctg acggtgttgc gcgaggtccc gaactcccgg gcgagagcgg
     6481 cctccagggg caggtggccg ccgtcgaaac caccgcgcac gatctcgcac cgcagcaggt
     6541 cggccacctg gcgggcccgg tccgcccgcc gccgtctcac atccgccagc tccatgggaa
     6601 agaagcctag cttcccggta ggaatatatg gataacgggc ggattacgcc accccggccg
     6661 gccgccctgc accgccgacc tggtcatatg cccgcagaga gccgagaacc gccacccctt
     6721 caccaccttg acgagcggcg acgcctcctg ggcacgagcc ggagaccggg ccgggaaggt
     6781 ccgggtccgg ctcgcggaag gaagcgggaa ggcgggcttc ctactgtcct ctcgtcgccg
     6841 gtcaccggta gacgtcgccc ggcggcccgc cgggaccgcg cgacgaaagg ctgaaaccgt
     6901 gagcaccaca cagacccgcg ccccgcgcaa cggcgtcggc acccccacgc tcttcgcgac
     6961 cctgaacgcc gtgaagggcg atccggaacc ggcgaagttc cggttccgcg cgaccaacac
     7021 ctggatcagc ggcacgcaca accgcagcgc gatccgcggg tcaccttgcg gatctccggc
     7081 gacgcgcccg ccgacgtgct gcacgggctg gtcgagcagt cccgggcggt tcggcggtct
     7141 atgacgtgcc gacgaacggc acgcgggtgg acatcgacgt ggtcacggcc tgagaacggg
     7201 cccgcggtga gagcggccgg gcccgcgccg acgcacgccc gcgcggggag tcacgggcat
     7261 cttctatccg aagcgggcac cggccggcgt ccgtcactca ccggaacgcc cacagcgcgg
     7321 gagcccgctc cttcaggccc ttgagggttg agaagccgac tgctcgcgca tgccccaggg
     7381 agtgccgtac gccgtgagca ggtcgaggaa gggccgcggc gggaaggcct ctggccccag
     7441 gactccggcg ccggaccagg cgccggaggc catcagttcg agggccacga ccgggttcac
     7501 ggcggtctgc cacacgaccg cctgggagcc gtactcgcgc atggaccact ggttgtcgac
     7561 cacgtggtag aggtacacct cacgcgggcc gccgtccttg gtgcccctga cccaggtgcc
     7621 cgcgcaggtc ctgccgtgca tgcgctcccc gagcgtcgcc gggtcgggca ggcacgccgc
     7681 gaccacgtcc cgcggggaga cctggacggg tacgccgtcc gcgccggcga cccggaccgg
     7741 ctcggtgcgg tccagcccca gcaggtgcag ggtccgcagc gtctggatga actcctcgcc
     7801 cagcccgtac ttgaaggtca cccgctcggt gtcgacccag cggggcatga gcaggacctc
     7861 ctcgtgctcg acgttgacgc actcgaccgg gccgatgccc tcgggaaagt cgaacacctc
     7921 gggctcgctg aagggcgggg tggtgaacca tccccggccg ttctcgtaga cgaccggggg
     7981 gttcaggcac tcctcgatcg tggtccagat gctgaacgac ggcgcgaagt cgtatccgtc
     8041 gacggtgagg tcggcgccgt cccgcacgcc gatctcctcg atgtggtcga aaagctcgtc
     8101 ggcggcgtac cgggcgaaca cgtccgagag gccgggctcc acgcccatgc cgaccagcgc
     8161 gagccggccc gcccgctccc actcggcggc cgtctcgaac tgggcgtcgc ccagcttgac
     8221 cccgcactcc tcgtgcgggc ggtccgggtg aggccgcgac agcgacatcg ccatgtccag
     8281 gtagtgcgcg ccggccgtca gcgcggcccg gaacaacggc atgacgaacc gggggtccgt
     8341 ggcgttgagc aggacgtcgc agcggtgctc cgccagcagc gccgcgacgg cggcctcgtc
     8401 tcccgcgtcg gcccggcacg cgctgaagcg ggcgcccgcc tccccgagcg cggcgacggc
     8461 ggcctcggca cgggacaggt cgtagtcggc gacgaccatg tgctcgaaga aggatctgcg
     8521 ggccgcgatc cgggtgatcg ccgtccctac tccaccggct ccgacaagca gcacgcgcat
     8581 ggtacaaaca tccatttctt ggacaaaggc ccctgatgtg tcgatccaac gccacgccta
     8641 cccggaacgt caatggtgtt ggcataaggt gggagcgagg agagggggac gccgtgccga
     8701 agccggtggt gggcgagtcg gcgcgccgga ggcgccgtcc gacgaagagc ggtgtggtcc
     8761 tctcggaggg cctgatcgtg cggaccgcgc tgcgcatgct gcgcgagcac ggccccgccg
     8821 ggctcaccgt acggcggctc ggcatcgccc tgggcgccga ccccagcacg ctctaccgct
     8881 acttcagggg cgtcgacgac ctgaccctcg ccatcggcga cctgctcatc gggcgggcga
     8941 tggacggctg gcgcggcggc ggcgactggc gcgccggact tcgcgacctg ggcctgcgca
     9001 tccacgccgc ctacctcgaa cacccgcagg cggcggtgct gaccgccagc cgcatcagcg
     9061 gcaaggcgca cgagatcgcg gccgacgagg cgatcctcgg gctgctgcgc ggcgcgggat
     9121 tccccgacca cgaggcggtc ggcgtctacc acaccttcat cgacatgacg ctggccttcg
     9181 cggcgctgga cgccgcctcc ctggcgctgc ccgccgaggc gcgcgaggcc gacgaggaga
     9241 tctggcgggc gacctacgcc cgcctgcccc gggccaccca ccccaacatc gcggcggtct
     9301 cccccctgct ggtcgcccac atgaacgaca gtgcctatcc cgccgccctg gagatgctcc
     9361 tcgacagcgc cgcgtcacgg ctcacgcgcc ccgtctcccc gccggccgag cccggccggg
     9421 cctgagccca ccggcgtcct agggggtggc cgccgggcgg gccaggggca ggcggggctg
     9481 gacgggggca gggcagggaa tcgtggaggc agccgggggt gagtaccggg caggcgggga
     9541 caggggccgc ctggacaagg gtgcctaggg gttttccacg cctatcgctc cttttagtgt
     9601 gattggtgtt tcagcagaaa ggcgagttgg cctcattcga gttcgcttga accgggcggg
     9661 ctcggatcga agccgctcga atcgcgaccg tcgcggcggt attcccttcc ctcacatcac
     9721 gagagcgccg gcgattccgc gaggacgccc cggtccgcct ctcgccgcac gaatatcccg
     9781 aaaacccgtt tatccccgaa ccccgctcat ccccgaaccc cgctcatccc cgaaccccgc
     9841 ccaacccaga aaacccgctt atcccggaag accacgttcc gcgccccgag gaaggagcag
     9901 ccatggagga gacgaccgtt ccggccgagg cgacggagga gctgacggcg gagacgctga
     9961 tcgaagaggt gtcgatcgac ggcatgtgcg gcgtctactg atgaccgatc ccctggacga
    10021 ggcctgggag ctgtctcccc gggtggcgct gcggccggag ccgttcgggg cgctggccta
    10081 ccacttcggc aaccggcggc tgaccttcct caagcgcccc gagctggtct cggtgctccg
    10141 cgacctctcc ggcgagcccg acgcccgtgc cgcgctgacc cgcgccggcg ttcccgccgg
    10201 ccggtggccc gcgtacgcgg aggccctgcg gggcctcgcc ggcgcggaca tgatcaggcc
    10261 gcgcggacgg acggaggcgg accggacacg gccgccccac gtcacggcag caccgggcac
    10321 ggcaggccag agcaccacag cccagagctt gccggccaag aacgcggcgg cgcgctccgg
    10381 gacggcgagc gggtcgctgg ccaagcggtt cgagtacggc ctggccgcgc ccatctgcct
    10441 gacctgggag ctcacctacg cctgcaacct gtcgtgcgtg cactgcctgt cgtcgtcggg
    10501 gcggcgtgac ccgcgtgagc tcgccaccgc ccagtgcgag gcggtgatcg acgagctgca
    10561 ggccatgcag gtcttctacg tcaacatcgg cggtggcgag ccgaccgtgc ggcccgactt
    10621 ctggcacctc gtgcagtacg ccgtgaacca ccaggtcggg gtgaagttct ccaccaacgg
    10681 ggcgcgcctc acccccgagc gggcgcggtt cctggccgcc acggactacg tcgacgtcca
    10741 ggtgtcgctc gacggcgcca cccccgaggt caacgaccgg gtccgcggcc cggggtcgta
    10801 cgagaccgcg ctgcgggcgc tcgacaacct cgcagcggcc gggttcgccg acgcgaagct
    10861 ctcggtggtc gtgacccggg agaacgcggg ccagctcgac gagttcaagg ccatcgccga
    10921 cgagcgcggg gcgacgctcc ggctcacccg gctgcgcccg tcggggcggg gcgccgacgt
    10981 gtgggacgag ctgcatccga cggcggccca gcagcgcgac ctgtacgagt ggctgctgcg
    11041 cgcgggcgag gacgtgctca ccggcgactc gttcttccac ctcgcggggt acggcgagtc
    11101 gctgccgggg ctgaatctgt gcggcgcggg ccgggtggtc tgcctgatcg accccctggg
    11161 cgacgtgtac gcctgcccgt tcgcgatcca cgaccgcttc cgcgccggca acgtgctctc
    11221 cgacggtggg ttcgccgccg tgtggcggga gtcggagctg ttcggggatc tgcggtcgcc
    11281 gcagacctcg ggcgcgtgca ccggatgcgg gttcttcgac tcctgccggg gcgggtgcat
    11341 ggcggccaag ttcttcaccg gcctgccgct cgacggaccc gaccccgaat gcgtgcaggg
    11401 gcacgccgcc gccgcgctcg cggcgggcga ccgtacggtg ccccggccca gccaggacca
    11461 ctcccgccgc cccggcgccc ggcgggccgg agccgggccg gttccggtgc gcatcggacg
    11521 ccgtccctcc cccgccgcgc cacccgtgca cccacccgtg cacccacccg tgcacgcgtg
    11581 cgcggagagc ccgctcgccg ggttcacccc ctcatccgac gtgtagcaga aggacgatag
    11641 ctcgtggccg cgaacgcctg gttcgagacg gttgcggagg cgcagcgcag ggcgcggcgc
    11701 cacctgccga agtcggtgta ctccgcgttg ctcgccggtt cggaaagggg ccggacgtac
    11761 cacgacaaca cggccgcgtt cgccgaactc ggcttcgcgc cgcacgtggc cgacctgccc
    11821 gccaagcgcg acctggccac caccgtcatg ggacagcaga tctccctgcc ggtgctcatc
    11881 tcccccaccg gggtgcaggc cgtgcatccc gacggggagg tcgcgatggc ccgcgccgcc
    11941 gccgcgcgcg gcacgatcat ggggctgagc aacttcgccg gcaagcccgt cgaggaggtc
    12001 gtggccgcca acccgcagac cttcttccag atgtactgga tgggcacccg ggagaccatg
    12061 gccgggcaca tggagcgggc ccgcgacgcc ggggtcaagg ggctgatcgt gaccctggac
    12121 tggacgttct cccacggcag ggactggggc agcccctgga tccccgaccg cctggacctg
    12181 aaggcgctcg cccgcttcgc gccgcagggc ctgacccggc cgcgctggct gctcggcttc
    12241 gccaggaccg gccgcctgcc cgacctcacc gtccccaacg tcgcggagcc cggccgtccc
    12301 gcgccgacct tcttcggcgc gtacggcgag tggatgcaga ccccgccgcc cagttgggag
    12361 gacgtgcggt ggatgcgcga gcagtgggac ggcccgttca tgctcaaggg cgtctcccgg
    12421 ctcgacgacg cccgccgcgc ggtggacgcc ggggccaccg cgatctcggt gtcgaaccac
    12481 ggcggcaaca acctggacgg cacgcccgcg accatccggc tgctcccggg catcgcccgg
    12541 gtggtcggtg accaggccga ggtcctgctc gacggcggcg tacggcgcgg gggcgacgtg
    12601 gtcaaggccc tggcgctcgg cgcgcgggcc gtgatgatcg gacgcgcctc gctgtggggc
    12661 ctcgcggcga acggccaggc gggggtggag aacgtgctcg acatcctgcg gaacggcatc
    12721 gactcggcgc tgctcgggct gggccgcgcg tcgatccacg acctggagcc cggcgacgtg
    12781 ctggtcccgc cggggttcac gagggagatc ggcgtcaccg gggagatcgg gtctccgcgc
    12841 gatgcgtgag aggggcgacg gcgaaggcgg cctggcggac ctgtcgtggc ccgcgttgcc
    12901 gccctcgccg accgtgctgg tgccgctcgg ctccaccgag cagcacggcc cgcacctgcc
    12961 cctgcacacg gacacggcgg tcgcggcggc ggtggcgagg gccgccgccg cccgccttct
    13021 cgcggcggct ccggacgacc ccgtcctcgt ggcgccggcg gtcgcgtacg gcgcgagcgg
    13081 cgagcaccag gacttccccg gcaccatgtc catcgggacc gccgccctcc gcttcctgat
    13141 cgtcgagctg gtccgctccc tgtcccgctg ggccgggcgc gtcgtgatcg tgaacggtca
    13201 cggcgggaac ctcgacgccc tcgcggccgc gacgtcgctg ctgcgtgagg agggccgaga
    13261 cgtgtgctgg gcgccgtgcg cggccggacc ggccgacgcc cacgccgggc acaccgagac
    13321 gtcgctcgtg ctgcacctgg ccccggatct ggtggacatg acccgggcgg agccgggcaa
    13381 tctcgccccg ctcgccgaga tcatgcccgc gctcgcggcg aggggcgtcg ccgccgtgtc
    13441 gccctccggc gtgctcggcg atccgaccag ggccaccgcc ggcgagggac gccgcctgct
    13501 cgacctgatg gccggcgacg tcgccgcccg catccggcac ggacgagccg accacaccgg
    13561 ccgcctcctc cccgcacccg cgacggtctc cccgacatga gccaggtgag cggcgggagc
    13621 gggccggagg agggtcgtcg ggtcgccctg gtgacggggg cggcgcgcgg gatcggcgcc
    13681 gccgtcgtgc gggcgctgtg cgccaggggt taccaggtgg tcgcggtcga ctcgtgcgcg
    13741 gcggacggcc cggcccggcc cgccggggtg acctacccgc tcgcggccga cgcggacctg
    13801 aagtcgctgg ccgacgagct ccccggcgcg gtggttccgt acatcgcgga cgtgcgcgac
    13861 cgcgacgcgc tcgcccgcgc cgccgccgtg gcggcggacc gcttcggccg gctggacgcg
    13921 gccgtggccg ccgcggcggt catggccggc ggcctgccgc tgtgggagac accggacacg
    13981 cacgtacgcg cgctgtggga gaccgacgtg ctcggggtgt ggaacaccgc cacggcgtgc
    14041 gtcccgcaca tgctggcggg gcccgagccg ggagcgtgcc gcttcgtcgc ggtcgcctcg
    14101 gcggccggtt cgcacgggct gttccgcctg tcggcgtaca cgatggccaa gcacgcggtg
    14161 gtcgggctgg tgcgcggcct cgcggccgac ctggtgggca ccggggtgac cgcggcggcc
    14221 gtctcccccg gttcgacccg cacgcggatg ctcgccgcca ccgccgaggt gtacggcctg
    14281 gacggcgtcg agggcttcgc ctcgtcccag ctgctgcgcc gcctgatcga gcccgccgag
    14341 atcgccgaga ccatcgcgtt ctgctgctct cgcgagggag gcgtcgtcaa cggatcggtg
    14401 atatcggcgg acggaggctt cacgccatga ccctgaccaa ccgcacccca cgcaaccgca
    14461 ccccacgcaa ccagaccccc cgcaaccaga cgtcgcgcga ccggaccacc ggcgaggaga
    14521 gcgccggcaa gcaggtccac ggcggcccgg cgccgagcga ccctgcgctg agtgaccctg
    14581 cactgaacga ccgggctgac ggcggcctcg gcgacgagga aaagagtgga ggcacggacc
    14641 cccgtgcggc cggccggctt ccggacggtt tcacggtcag gctgaaccgg cgggtccggg
    14701 tccgcgacgg cggccgcacc ctcgtgggcg gcgcgccgac gcgcgtcatg tacctcacca
    14761 aagccgccgc ggacctgttc gacgggcgca ggttgcgggt ccgcggccgc gtctcggccc
    14821 tgctcgccga ccggctgctg gaggcgggcc tggccgatcc cgtcgtcgga gacctgcccg
    14881 ctctcgaccc atcccaggtg acggtggtcg tgccggtgcg cgaccgtccc gccgcgctcg
    14941 accggctgtt gtcgggcatc ggcggccggc accgcgtgat cgtggtggac gacgcgtcgg
    15001 ccgccccgga ggccgtcgcg gaggtcgcgg cggcgcacgg cgcggtgctc gtcacgctgg
    15061 cggagaacgc cggccccgcc gcggcccgca acgcgggcct gcgccgcgtg accacgccct
    15121 acgtcgcctt cgcggactcc gacgtggtcc tcgaacccgg cacggtgccg accctgctcc
    15181 ggcacttcgc cgacccccgg gtcgccgtcg ccgcgccccg cgtgctgggg ctccgcctgc
    15241 cgggccggga ccgctggatc ctccgctacg aggacgcgcg gtcctccctc gacctgggcc
    15301 tcgaccccgg catcgtacgc ccgagggcgc cggtctcctg ggtcccgagc gcgttcctgc
    15361 tggcgcgggc cgacgcgctc ggccacggct tcagcgccga gatgcgcgtc ggcgaggacg
    15421 tcgacctggt gtggcggctg gcgcgggcgg gctggcgggt gcgctacgaa ccggccgcga
    15481 cggtgcggca cgagcaccgc acggtggtcc acgactggct gcggcgcaag gccttctacg
    15541 gcaccggcgc cgacctgctc gcccggcggc acggccgcga cgtggccccc gccgtgctgg
    15601 cgccgtggag cgcggccttc gtgctggcgc tgctggcgca gcggcggtgg tccctgccgg
    15661 ccgcggcgct gatcttcgcg ggcacggccg cgacgatgtc ggccaaggtc aggcggagcg
    15721 agcacccggt gcgcctcgcc ctgacgctga cgtccgacgg ggccgtcgcc gccgtctggc
    15781 aggcgacggc gctcctgctc cgccactggt ggccgctcgc cgccgtcggc tgcctgctgt
    15841 ccaagcggct ccgccgggcc gtggccgccg tggccgtcat ggacgcgctg accgatctgc
    15901 ggcgtaccgg cgcgcatctc gacccggtcc gctacgccgt ggcccggcgc ctggacgacg
    15961 cggcgtacgg gtcgggcctg tggctcggcg cgatccgcgg gcgctcggtg cgcgccctgc
    16021 tgcccgacat ccggtggaag acgcgttcgt cgaggccgtc ccgatagaag ccgtccggct
    16081 ggagagcctg gtgagccgcc gccccgggag cgcccgggac ggcggccgcg tcctcaccgg
    16141 acaccggtga gcgcgtcggt gcggtgacac gccacccagg accggggcga gccgtagtcc
    16201 ggcaggcgca gccggggccg gtgcctcttc ggccgctcga ccggcccggg ttctccgccg
    16261 tccccgcggt ccgccgcgac ggcgtacagg gcgcacacca gtgcgcagat ctcctcctcg
    16321 gtgggatttc ccgcgaccac ccgcagttcc tcgccgccca tcacatcggg gggtttccgt
    16381 gcttgcgccg cggctccgac gcctgcttgg cccgcagcag gccgagcgcc ctggtcaggg
    16441 tggccctggt ctcgcccggg tcgatgacgt cgtccaccag cccgcgctcg gcggcgtagt
    16501 aggggtgcat gagctgcctg cggtactcct ccacccgccg ggcgtgcgcc tcgacggggt
    16561 cgtccgccgc ggcgatctcc cggcggaaga tgacgttggc cgcgccctcc gcgcccatga
    16621 ccgcgatctc gttggtcggc caggcgaagg acaggtccgc gccgatcgac cgggagtcca
    16681 tcacgatgta cgcgccgccg tacgccttgc gcagcaccat cgagatgcgc ggcacggtcg
    16741 cggcgcagta cgcgtagagc agtttggcgc cgtgcctgat gatgccgccg tgctcctggt
    16801 cggtcccggg caggaagccc ggcacgtcga ccaggctgac cagcgggatg ttgaaggcgt
    16861 cgcacatctg gatgaagcgt gcgcccttct ccgaggcctt gatgtcgagc accccggcca
    16921 ggtgggcggg ctggttggcg acgatgccgg tgacgtggcc gtcgaaccgg gccagcccgc
    16981 agatgaggtt ctgcgcccag cccgcgtgga actcgaagaa ctccccgtcg tcgacgatct
    17041 cctcgatcac cgccgacatg tcgtaggaga tcgccggatc agccggcacc aggtcgagca
    17101 gggcgtcgtt gcgccggttc gccgggtcgg tgcaggtgat cgacggcggc agttcctggt
    17161 tgttggcggg cagcaaagac agcagatagc ggacttcctc aagacacgac acctcgtcgg
    17221 ggcagacgaa ccccgccacg ccggagactg atccgtgcac ccgcgcacca ccgaggtcgt
    17281 cggcgctgat ctcctcaccg gtcaccgcct tcacgacgtc cgggcccgtg atgaacatct
    17341 gggccgtgtt ctgcaccatg aacacgaaat cggagagagc cggcgagtac gccgctcccc
    17401 ccgcgcacgg gccgagcatg acgttgatct gtggaatcac ccccgacatc cgcacattcc
    17461 gccggaaaat accgccatag ccggcaagcg cggtcacgcc ttcctggatc cgcgccccgg
    17521 cgccgtcgtt caaaccgatc agcggcaccc cggcggcctc ggccaggtcc atcaccttgt
    17581 ggatcttctc ggcgtgtgcc tcgccgagcg cgccgccgaa catccggaag tcgtgggcga
    17641 agaccatgac gtcacgaccg tagaccttgc cccaccccgc caccacgccg tcggtgtacg
    17701 gccggcggtg ctccagcccg aaaccggtgg cccggtgctg ccggaacgcc tcgacctcca
    17761 cgaaggaccc gctgtcgagg agcacgtcca ggcgctcccg cacggtgagc ttgcccttgg
    17821 cgtgctggcg cgcggtcgcc tcctcactcg ggcccgaatg gatgctctcc ttgagcacct
    17881 gcaaatccgc cagccgttcc cgcatcgatt ctttcggcag gtcgctgacc cgcgtcacca
    17941 cggtccccat atgtattcct cctgacggca caaccgggtg ccctcagggt aaggcggccg
    18001 atgaacgcga caacacccct aaaaaggacg gcgaccgcgc caataggacg cgacccaaaa
    18061 agaaccggaa tcagtccgga tatcggtgtt ccggtccacc ggtggccgac cccttacggg
    18121 ccggccaccg gggaccacgg tgaaacagcg gtgggatgag ctcagccgac ggcggctccg
    18181 ccgaactcga ggaccgcggg caggtcggcc cggctggtga tgtcgagctt gcggtagatc
    18241 cgggtgaggt gctgctccac cgtgctcacc gtcaggtaca gctcaccggc gatctcccgg
    18301 ttggtgtgcc cggcggcggc cagcaccgcg acccggcgct cggcctcgct cagcagcgcg
    18361 gcgctgcccc cgctcagggc cggggccgtc tgcttgccgg tctcggccgc cgcctgcggg
    18421 ccgccgatct cggcgccgca ctccttcatc agggcacgcg cccggatcat gatcatgccc
    18481 accctccggt gctcgcccag gccgcggtag gcctccgcga ggtcgaagag cgatcgggcc
    18541 tgctcgtaac ggtcgccgcc gttctgcagc atgtcggcgg acttgcgcag gagcatcggc
    18601 cggtgccgca gctcgctggt ggccgccagc aggcggatcg tcatgccgag ctcccgcggc
    18661 gtgcgggagt tgcagcggga caactggtcc tcgatcaact tcgcggcctg ctcagtgcga
    18721 cccaactgca gacaggcctc ggcggcgccg gcccgccacg gcagcaggcc cggcacgtcc
    18781 aggccccacc tgccggtcag ttcgccgcag agctggaagt cccgcagggc cagcgccgcc
    18841 tgcccgaccg ccaggctgta gcggcccctg gcgtacaggt agtgcaggcc gtaccggctc
    18901 tcgaacatgg cgtccagcac gggccggtcg aggatgtcgt gcacgtcgtc gaacctgccc
    18961 atcgcggtca gcgcgatcac caggctgccg agcgggctgc ccaccgtgac accccagctc
    19021 gtgatgggca tgatctccaa cgcggcccgc gcgtactgct cggcgctgac catgtcgccc
    19081 tgccgtacgg ccatctcggc gcggatggcc gagagcctgg cctggcggct cggcgcgcgc
    19141 cgggacgacg cctcgacgct gaacaggtcg cacagcggcg cggccttctc cgcctgcccg
    19201 ccgtaggtga gcgcgagcag cgcgcactcc acggtgtcca tcgacatctc gtccaggcgc
    19261 gcgagccgca ggatgcgctc gacggcgatc agggcgccct cgtgcgggcc gttcacgagc
    19321 acctgcgcga gcgcggtcgc cgtctccagg cgccggcccg cggccaccga caccagggcc
    19381 gttccggtgt ccgcgcgcgg ctcgccgagc ttggacgcga aggcgggatg ggtggcgcgc
    19441 agccacgggc gcgcgatgtt gagctccatc accgtctcgg ggtcgctctc gtatcccgcg
    19501 gtggtcaggt gctcgatcac gtcctgcgcg tcgtcgtacc tgccgtgcca gagcagcgcg
    19561 cgggccagga ccacgccgtc gccgccgcgg aggtggccgc gctgcatcgc ctcggtgaga
    19621 tcggccagat gccggtcggg cgcctcgggg ttgatgcgcc actcggcacg caccagcatc
    19681 gtggcgatct tgaggcggcg ccgctcgtcg gtgcacgcct gccacgcgag gcgcaggtag
    19741 tccacgccac gctcggtgcg gccctcgcgc agcgcgtccc tggccgcgtc ctccagcacc
    19801 ggcaccaccc agggagcgtc gatcgggccc gcctggacga ggtgatcggc gatcaccgaa
    19861 gtggccgcgc cgtccgcgtg cgccagtccg gccgctcggc ggtgcaggtc gcggcgctcg
    19921 tcgaggccca cctcggccag cacggccgta cgggccgact cgtgccggaa ccggcccacg
    19981 ctgagcacgc cgccggccgt cagcgcgttc atcgccctgc tgatctgggt gtggcccagg
    20041 ccgagcagcc ggtcgagcga gtcggtctcg ccgagcaccg ccaggcaccg ggcgatccgg
    20101 agcagctcgg gctcgccgcg gtgcaggcag gtcagcacgg cctggccgta ccgctcgccg
    20161 accaccggct cgtccgccgc cggctcgccg tcccgttccc gcgcggacgg gccgccgagg
    20221 tagtcctcga tgagggcggc ggccagcagc gggttgccgg cgctgtaggc gtaccagtcc
    20281 tcggcgaagc ggaccgcgac ctcctccccc gcccgctcgc gcaccagcgt gtgcacgccc
    20341 tgccgggaca gcgggttgag ctgcacgact ccgcagtacg gctggcgcag caggtcggtc
    20401 cagaagaagg tgtcggtgct gcgcacgtag tcggagtagc tgaagatcac catgatccgg
    20461 gcgaagcgta cgcgccgggc gaggtacgcc aggcacagca acgacgggcg gtcggcgtag
    20521 tgcacgtcgt cgaccacgat caccagcgga tagcgctccg acagctccag cagcacggtg
    20581 cacagagcgt gcgcgatctg ggcgtccacc tggtcgaggg tctcggaggc gagcccggag
    20641 ctcatcgccg tccgcttgcc ctcctcgagc agcgcgagcg cgcgcacgcg ctcctcctcc
    20701 accagcggcg cctcttcgat gagctggccg agcacgccga gcggcaggtc ctgctccatg
    20761 cgggacgcgg tcgcggcgac gggcagggcg cccagctcca ccgcctgctc ggcgaacgcg
    20821 tcgagcagtg cgctcttccc ggtggcgacg gtgccgctga gcatcgcgac cttgcccttg
    20881 ccggtcagtg cggccgccag catctcctgt aacgaggcca gtgcctcgtc tctctcaatc
    20941 aagttcatca agcgggcccc ctccagctga gcttccgtcc ttacgaatgg acgcttgcct
    21001 gcttgttgga gacgatgttc tccccggccg cttttggatc gctatcgcgc ccttatccct
    21061 cgccgcgacg tcccctccgg gcatctctcg cagcgggacg ggacgcgccg ggccggagcg
    21121 tgccggagcg cgcgacagcc gcccgtcccg cccgtgacag gggcggaggc gggcgggagg
    21181 cgcgtcagaa cttcagcggg cggccggagg gagtgcgcag gtcgggcgct tcgtccctgg
    21241 cggcgggacg tcccgagggc cggctcttct tcagctgctt tcgcgtcatc gcgtcgtgcc
    21301 cttccgtgcg gggcccgggg acgagggccg gaggatggtc gtccgcggtc acgcggtggt
    21361 ggccagccgc cgcctgcgcg ggcgcgcgtg ctcgtgcgcg tcgcccagtt cgcggtggtg
    21421 cctgccggag ggctcggccc gccgggcggc ccggtggacg acggcctgca ccgcccggag
    21481 cgcggcggcc tcggcgtccc gctcgatccc cacgccccag tgcgtacggc ccccgaggcg
    21541 gcactcggcg tagaccgcga cgcccgcctc gacgtcccgc tccccctggc cgacgccggt
    21601 ccggtggacg gcgcggacct cgatccccca gtcggcgagg ctcacccgga gcgcctgcag
    21661 cgcgtccgcg cgctcggcgc ccacctcgaa gcctccgccg tccacgtgca gttcgacggc
    21721 gagcggctcc ggcccgaaga cgggcggccc cggcgggcac gtgtactcgc tctcgaacag
    21781 ctcccgcacc ccgctggggg tgatctccat gccttcgctg tcggcccgcc gctgcaccac
    21841 ccgggcgaag tcgatctgca ggtcgcgcgg caggtgcctg ccgtaccagg tgctcatgat
    21901 gtaggcgacg ccgcccttgc cggactggct gttgatgcgc accacggcct cgtaggtccg
    21961 gcccacgtcc gcggggtcga cgggcaggta cggcatggcc cagggcatct cgtcgatcgg
    22021 ggcgccgtgc gcggcggcct cccgctccag cgcgtcgaag cccttcttga tggcgtcctg
    22081 gtgggagccg gagaaggagg tgtagacgag gtctcccccg tacgggtggc gcgggtggac
    22141 cgggatgccg ttgcactcct ccacgacccg gcggatcgcg ttgaggtcgg agaagtcgac
    22201 gcccgggtcg acgccgtggc tgaacaggtt catgccgagc gtgaccagat ccacgttgcc
    22261 cgcgcgttcg ccgttgccga acaggcagcc ctcgatgcgc tgcgcgccgg ccatcatggc
    22321 cagctccgcc gaggcgacgc cggtcccccg gtcgttgtgc gggtggatgg agaggcagac
    22381 gtgctcccgc cgggacaggt tgcggtcgag ccactcgatc tggtcggcga agacgttggg
    22441 cgtgctccgc tcgacggtcg tcgggaagtt caggatgatc tcccgcccgg ggccgggctg
    22501 ccagacgtcc atgaccgcct cgcagacctc cagggagaag tccagctcgg tgtcgttgaa
    22561 cagctcggga gagtactgga agcccaggtc gcagtcgctc agcgtcttgt ccgcgtattt
    22621 catcatcagg cgcgtgccct ggacggccag gtccttgcac tcgtccctgg tcatcccgaa
    22681 caccagccgc cggaacaacg gcgacgtcgc gttgtagagg tggatggtgg cgcggggagc
    22741 gccttcgagg ctctccacgg tccgccggat cagctcgtca cgcgcctgca ccagaacgga
    22801 gacgcgcacg tcgtcgggaa tgaggtcgcg ctcgatgagc tcacgcagg
//
