LOCUS       NZ_VYTZ01000008        10270 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Microbispora cellulosiformans strain Gxj-6 Scaffold8, whole genome
            shotgun sequence.
ACCESSION   NZ_VYTZ01000008
VERSION     NZ_VYTZ01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora cellulosiformans
  ORGANISM  Microbispora cellulosiformans
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 421897)
  AUTHORS   Gong,X.
  TITLE     Screening of Novel Bioactive Compounds from Soil-Associated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 421897)
  AUTHORS   Gong,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) College of Land and Environment, Shenyang
            Agricultural University, No. 120, Dongling Road, Shenhe District,
            Shenyang, Liao Ning 110866, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008728085.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 02:05:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,471
            CDSs (total)                      :: 7,401
            Genes (coding)                    :: 7,312
            CDSs (with protein)               :: 7,312
            Genes (RNA)                       :: 70
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 7 of 89
            Pseudo Genes (incomplete)         :: 80 of 89
            Pseudo Genes (internal stop)      :: 8 of 89
            Pseudo Genes (multiple problems)  :: 6 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:06:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 177178
            Orig. end                         :: 187448
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VYTZ01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10270
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10270
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5270](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5270
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    148..162
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(747..1574)
                     /locus_tag="F5972_RS23380"
                     /old_locus_tag="F5972_23380"
     CDS             complement(747..1574)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544527.1"
                     /locus_tag="F5972_RS23380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23380"
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="WP_150935856.1"
                     /transl_table=11
                     /translation="MSKDFRFGIVAGLAPDAAAWTATARRAENLGYSTLLVPDTLGTLP
                     PLLAVTAAATATTTLRVGTFVLAAPYRNPRLLAHELAGVDFLSGGRLEVGVGAGRPAAE
                     QDARTLGMPYGTAGERVAQVRSVIGEVRTAFTEGGLSVRRQGPPILVAGSGPRMLALAA
                     EKADIVALAVPAVTSEEGLRGPVGTLRELAGARFDDIELSLNLFQVGDVPAPWLPEGSP
                     TIPDDAAAVLRGSPREMADTLLRRRDDLGISYVTVNGFYADAFAPVVELLTGR"
     gene            complement(1650..2555)
                     /gene="rarD"
                     /locus_tag="F5972_RS23385"
                     /old_locus_tag="F5972_23385"
     CDS             complement(1650..2555)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene="rarD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130561.1"
                     /locus_tag="F5972_RS23385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23385"
                     /product="EamA family transporter RarD"
                     /protein_id="WP_150935858.1"
                     /transl_table=11
                     /translation="MPDTRRGLLFGVAAYTMWGLFPLYWPLLKPSGAVEILAHRMVWSL
                     VVVVAVLAVRRHWSWIRTITRRQLILLTVAAVTVSVNWGTYIYAVNTGHVVESALGYFI
                     NPLVSVLFGVLVFRERLRVWQWAAVGLGALAVLILTLDYGRLPWIALVLAFSFGSYGLI
                     KKKAGVGSAESLTIETLVLLVPALAYLLVLEANGTGTFAHHGAGHALLLVAAGLITAVP
                     LLFFTASALLVPLTVIGLLQYIAPVLQFLCGVLIVHETMPASRWAGFVVVWLALSLFTW
                     DSIRAARTSRRERAAELQTV"
     gene            2690..3352
                     /gene="msrA"
                     /locus_tag="F5972_RS23390"
                     /old_locus_tag="F5972_23390"
     CDS             2690..3352
                     /EC_number="1.8.4.11"
                     /GO_function="GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130562.1"
                     /locus_tag="F5972_RS23390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23390"
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_150935860.1"
                     /transl_table=11
                     /translation="MGWLFGRDKTTMVEPTDALPGRAEIMPVATRHQVLGAPLAPPYPE
                     GTEIAEFGLGCFWGAERKFWQTPGVVSTAVGYAGGYTPNPTYEEVCSGLTGHTEVVRVV
                     FDPAQVSYEELLKVFWEAHDPTQGMRQGNDVGTQYRSTVYTHGPEQLEAALASQEAYQK
                     VLTDAGYGEITTEIAPAGEFYFAEDYHQQYLFRNINGYCGIGGTGVACPIGLTTGEA"
     gene            complement(4225..4524)
                     /locus_tag="F5972_RS23395"
                     /old_locus_tag="F5972_23395"
     CDS             complement(4225..4524)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F5972_RS23395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="F5972_23395"
                     /product="hypothetical protein"
                     /protein_id="WP_150935862.1"
                     /transl_table=11
                     /translation="MAASIPHLDECLSPHWEMRQQDHVKLVWHEPFEGVRRVRALAWTC
                     DCRAVVYELLQSGGQAFIRKTTQTEPTPKVEETHRWSIEEARRAWHALLLGEMR"
     gene            complement(4538..4879)
                     /locus_tag="F5972_RS23400"
                     /old_locus_tag="F5972_23400"
     CDS             complement(4538..4879)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F5972_RS23400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="F5972_23400"
                     /product="hypothetical protein"
                     /protein_id="WP_150935864.1"
                     /transl_table=11
                     /translation="MRQNTISNRTADQQAGILAALSDLLRSQEVRTRLISHITVRMTGF
                     PIWDPAVSELDIYGLGGQVATVTIDQENKPRRFLIKLPKSGTVVPLSTVDQPQSAVRWI
                     ANWANGGPR"
     gene            5001..5270
                     /locus_tag="F5972_RS23405"
                     /old_locus_tag="F5972_23405"
     CDS             5001..5270
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012610.5"
                     /locus_tag="F5972_RS23405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23405"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_076437814.1"
                     /sec_met_domain="TIGR03651 (E-value: 4.3e-05, bitscore:
                     18.4, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEFRPDQLKWEAVLEEIRRRIRTGEYQVGHPIPGEPRMAEEFGI
                     AKGTARRVINELLKAGDVYTVLGKGTFVADPETGGPPRRDTEDE"
     gene            complement(5467..6864)
                     /locus_tag="F5972_RS23410"
                     /old_locus_tag="F5972_23410"
     CDS             complement(5467..6864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547010.1"
                     /locus_tag="F5972_RS23410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23410"
                     /product="replication initiator"
                     /protein_id="WP_191910281.1"
                     /transl_table=11
                     /translation="MSELPPLSPDVLAAAQRAAMPDFARWQEMVRATGGCAQPIRLQGR
                     RITFDAGLEVLDVYRTADEPTGYLLTACGNRRASRCPACSEVYRDDTYHLILSGLRGGK
                     GVPEDVSAHPRVFATFTAPSFGAVHARRVKSDKVLPCRPRRDNPVCAHGRPEGCGVRHA
                     DDDPQVGQPICVDCYGYRDAVLWNAHAGELWRRFTQALPATFARHLGISRAELRRRLRL
                     SYAKVAEYQARGLVHFHAVIRLDGPDGPSDRPPDWATVPVLQEAIRETAAAVSVPVPDD
                     DPSFVGRWGTQLDVDPIYVATSVEGIADQRVAAYIAKYATKGAESAGTVDRPIREVPDV
                     ARLDVTEHARRMIYTCFALGNDPAYRLIPLRQWAHMLGYRGHFSTKSRHYSTTLSALRR
                     VRADHQAERARERRGLPDPAERETVTVGEWRFAGSGYRHGEHLWAELIRQRVATARRIA
                     RERGESA"
     misc_feature    7291..7311
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            complement(7680..9068)
                     /locus_tag="F5972_RS23415"
                     /old_locus_tag="F5972_23415"
     CDS             complement(7680..9068)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012464.5"
                     /locus_tag="F5972_RS23415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F5972_23415"
                     /product="recombinase family protein"
                     /protein_id="WP_191910282.1"
                     /transl_table=11
                     /translation="MATHTPPIPVVSYARISADTKRDEHGVQDQHKLNRETAARYGWTV
                     VHEFTDNDKSAAKADVVRDEFEAMLKVLRSGELPDGPEVRGVVIVAEDRLARRPGDYER
                     FVEAITYQDGRVFADARGQKDLYSEDTESMGLFGAVISKMEVRKMQRRIRRSHRARAEQ
                     GLPVGGTRPFGWLPDRLTLDPQEAPLVRQAILDLVAGRSLNSIVNQWQRGDVKTSLGNR
                     WTSRSLKVTLTNPRVCGWREMNGELIRDSEGNPVVGQWQPIVTPEQWMAVKSIFDARKG
                     HFVYRDGRLGPILPRDFKEPRHLLTGFLRCGRTQPDGALCNASLRVTHQKHCVQHIYIC
                     PAKTVGGCGGLGRRGDMVDLYVSEAVLAKLEEVQLASSTGPTEWPGQQDYEDAKARLEE
                     LRAQWTAGNISNELFFSAAPDLEKRISRPSHGRAELRGDSSTTTGPLGYRRGRDTAAVV
                     PIGE"
     misc_feature    complement(8541..8543)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    9409..9422
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    complement(9488..9502)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.65"
                     /tool="antismash"
ORIGIN
        1 tgccgaagag tcctgaccag gtcggcacga gcctgaccgt acggccgcgc gccgcgaggg
       61 tgcgccgggc catgtcgacg aggtcctgtg gctcggggcc ggcaacgtcg gcatggcgtc
      121 cccgcggctc gcccgccgcg atctcggcga ggacgtcggc cacgtccgcg ggcgcgatcg
      181 gctgcacgag cagtggtgcg atcgtcgcga cgccgtcctg ttcggtccag ctcaccaccg
      241 tctcggcgaa gtcgtggaac tgcgcgaccg ggacgatcgt ccacggcacc ggcccggcgg
      301 acacgaggcg ctcctgctcc cgcttgccgg cgtagtgggc gttgccctcc acccggtgga
      361 tgccgatgat cgacagcagc gcgtggtggc ggacgcccgc ccgctcctcg gcggcgagca
      421 ggttgcgggt ggtcgtgccg aagtacgcca ccgcctcgtc ccggtcggtc gccgccgaac
      481 tgatcgcgtc gacgaccgcc tccacgccgt ccagggcctt gtcgagccct tcgcccgtca
      541 tcaggtccac gccgagggag cggctgacgc gtacgacgtc gtgcccgtcc cgttcgagag
      601 cggcgacggt gagcgatccg atgttcccgg tggcgccggc gactgcgatt cgcatcgtct
      661 tctccttcgc tcgatgccac ggatgctatc tccggccgac caggcgagat ccgtcgaggg
      721 tgcccgagga ctgccggcgc agcgcgtcag cggccggtga gaagctcgac gacgggagcg
      781 aacgcgtcgg cgtagaagcc gttgacggtg acgtaggaga tgcccagatc gtcgcggcgg
      841 cgcagcagcg tgtcggccat ctcccggggc gagccgcgca gcacggcggc cgcgtcgtcg
      901 gggatggtgg gggagccctc gggcagccac ggggcaggca cgtcgccgac ctggaagagg
      961 ttcaggctca gctcgatgtc gtcgaacctc gccccggcca gctcccgcag cgtgccgacg
     1021 ggcccgcgca ggccctcctc ggatgtgacg gcgggcacgg cgagcgcgac gatgtcggcc
     1081 ttctccgcgg cgagcgccag catgcggggg ccggagcccg cgaccaggat gggcggcccc
     1141 tggcgccgca cgctgaggcc gccctcggtg aacgccgtcc gcacctcgcc gatcaccgac
     1201 ctgacctggg cgacccgctc gcccgccgtg ccgtacggca tgccgagcgt gcgggcgtcc
     1261 tgctcggcgg cggggcgtcc ggcccccacg ccgacctcca gccgtccgcc ggacaggaag
     1321 tcgaccccgg ccagctcgtg cgccagcaac cggggattgc ggtacggcgc ggccagcacg
     1381 aacgtgccca cgcgcagcgt cgtcgtggcg gtggccgccg ccgtcacggc gagcagcggc
     1441 gggagtgtcc ccagcgtgtc ggggacgaga agggtcgagt agccgagatt ctcggcgcgg
     1501 cgggccgtgg ccgtccacgc ggcggcgtcg ggggcgaggc cggcgacgat cccgaagcgg
     1561 aagtccttgc tcatgggctg atcctcgccg ccgcggacgg ccgaggtcgt ccccccgtcg
     1621 gtggtgggag ccgtactccc cggggcgtac tacacggtct ggagttcggc ggcgcgctcg
     1681 cggcgggacg tgcgggcggc gcggatgctg tcccaggtga agaggctcag cgcgagccag
     1741 acgacgacga agccggccca ccggctggcg ggcatggtct cgtggacgat cagcacgccg
     1801 cacaggaact gcagcaccgg agcgatgtac tgcaggaggc cgattaccgt gagggggacc
     1861 agcagtgccg aggcggtgaa gaagagcaac ggcaccgcgg tgatcaggcc ggcggccacc
     1921 agcagcaggg cgtggccggc gccgtggtgg gcgaaggtgc ccgtgccgtt cgcctccagg
     1981 acgagcaggt aggcgagggc cggcaccagc agcacgaggg tctcgatggt caggctctcg
     2041 gcgctgccca cccccgcctt cttcttgatc aggccgtacg agccgaagct gaaggcgagc
     2101 acgagggcga tccagggcag gcgcccgtag tcgagcgtga ggatgagaac cgcgagcgcg
     2161 cccagcccga ccgcggccca ctgccacacg cgtagccgct cgcggaagac cagaactccg
     2221 aagagcacgc tgaccagggg gttgatgaag tagccgaggg cgctttcgac cacgtggccg
     2281 gtgttgacgg cgtagatgta ggtgccccag ttcaccgaga ccgtgacggc cgcgacggtg
     2341 aggaggatca gctggcggcg ggtgatggtg cggatccacg accagtggcg ccgtacggcg
     2401 aggaccgcga ccaccacgac gagcgaccag accatccggt gcgcgaggat ctccaccgct
     2461 ccggacggct tgagcagggg ccagtagaga gggaagagac cccacatcgt gtacgcggcg
     2521 acgccgaaca ggaggccacg gcgggtatca ggcatgtcgc ctatcttcgc tgcttacctg
     2581 gattcaggac aaataaggac ctctatgccc ggagtttagg actccttacg tgaggaacaa
     2641 cgcggccggg tgggtggttg gtgatggcag acagaagggg aggtccgtga tgggctggct
     2701 gttcggcagg gacaagacga cgatggtgga gcccacggac gcgctgccgg gccgggccga
     2761 gatcatgccg gtggccaccc gccaccaggt cctcggcgcg ccgctggctc cgccgtaccc
     2821 cgaaggaacg gagatcgccg agttcgggct gggctgcttc tggggcgccg agcggaagtt
     2881 ctggcagacc ccgggggtcg tctcgaccgc ggtgggctac gcgggcggtt acacgcccaa
     2941 ccctacgtac gaggaggtct gctcgggcct caccgggcac accgaggtcg tccgggtggt
     3001 cttcgacccg gcccaggtgt cgtacgagga gcttctcaag gtcttctggg aggcgcacga
     3061 ccccacccag ggcatgcgcc agggcaacga cgtcggcacg cagtaccggt cgaccgtcta
     3121 cacccatggc cccgagcaac tggaggccgc cctggcgtcc caggaggcgt accagaaggt
     3181 gctgaccgac gccgggtacg gcgagatcac caccgagata gcccccgccg gcgagttcta
     3241 cttcgcggag gattatcacc agcaatacct tttccgcaac attaacggct actgcgggat
     3301 cggaggcacg ggcgtcgcgt gtcccattgg cctgaccact ggtgaggcct gacctgcggt
     3361 aactgactga tcctggccct tggtcgcccg gcaagcccgg tgccgatcag gggccagagc
     3421 cgttttcggc gagaaccttc actacaaggt caaggcgagc cgcctgcggc ggctcgcagc
     3481 gcgccgggcg gccagcgggc cgggcatgcg gcctgggggc cggtcccggc ccgcgccgcc
     3541 cgcgcttaat ccacagtggc ccgcagggag ttgatcattc cgaatggtct ttaggagggc
     3601 gtccgccccc aacgcccatc ccagggcgtt cttcgctcca agcaatgatc gtggcgggtt
     3661 tccacacggg ggaccggcca aatatcgcgt cgggttccgg caggtcgcct ggcttcggcg
     3721 tccccttcct gcggttgaga ctggcccggg cgttgtagac tcgcgccgtg ttgacttgga
     3781 cattgaggaa acctgcaata tcggtgagtg tccagtaaac cgcctctgta tccggtctcc
     3841 gcggcacctc tttctctgct tctttcatag atacatagta tctagaagat aatctctacc
     3901 tactccgaat cgtcaggaga gtgtggctgg tgtaggtatg gaagcagact gcaggcactc
     3961 agattagaca gtcagccgaa ggccgctccc cgcccccgct gctcgattgt tcgtggccct
     4021 tggtcggtgt caacgccgtc tgtgcgggtt gggtgtcggg ttgcggtgta cgtcgctggt
     4081 gaacggacac atgaagacgg cattgacacc tcggtcgggc cactgatgtc ggcagctatc
     4141 gggggcgggg gaggagtggc gcgggtcgta gacgcgagtg cggccccgcc cgaggtgagc
     4201 ggggccgcgt gagggctggg ccgtctatcg catctcgccg aggagcaggg catgccacgc
     4261 cctgcgggcc tcctctatgg accagcggtg tgtttcttca acctttggtg tgggttcggt
     4321 ctgggtggtc ttgcggatga acgcctgtcc gccggactgg agtagttcgt agacgacggc
     4381 tcggcagtcg cacgtccacg caagcgcccg cacccgccgg accccttcga acggctcatg
     4441 ccagacgagt ttcacgtgat cttgttggcg catctcccag tgcggactca ggcactcgtc
     4501 caggtgcggg atggaggcgg ccaccagtac cgccgcctta ccgtggcccg ccgttggccc
     4561 agttggcgat ccaccggacg gcggactgtg gttggtcgac cgtggacagc ggaacgacgg
     4621 tcccggactt cggtagtttg atcaggaagc ggcgcggctt gttctcctgg tcgatggtga
     4681 ccgtcgcgac ctgtccgccg aggccgtaga tgtccagctc cgagactgcc gggtcccaga
     4741 tcgggaaccc ggtcatccgc acggtgatgt ggctgatgag ccgggtacgg acctcttggg
     4801 accggagcag gtcgctgagg gcggccagga tgcccgcctg ctggtcggcg gttcggttgc
     4861 tgatcgtgtt ctgacgcata gctctcccat ctgtctcgct gacgggttta gcgtacgccg
     4921 accttcctag tcctactcct aggtacctct aggtacctgt ggtaacgaca tggaacgctt
     4981 tcgttcctac tcttgagccc gtgatcgagt tccggcctga ccagctcaaa tgggaagccg
     5041 tcctcgaaga gatacgacgc cgtatccgga cgggcgaata ccaggtggga caccccatcc
     5101 ctggcgaacc ccgcatggcc gaagagttcg gcatcgccaa gggcaccgca cgccgtgtca
     5161 tcaacgaact gctcaaagcc ggagacgtct acaccgtgct cggcaaggga acctttgtcg
     5221 ccgaccctga gaccggcggc ccgcccagga gagacaccga agacgagtga aggcccccga
     5281 acgagtccag gggccttcag ccttctatcg gttgtcagcg agacgatgga aggcgcacca
     5341 agcgtagagc cgacgagcca agggcagtag ccaccggccg tggcgccgcc ggcccaaccg
     5401 cttcgcggcc cttcgggtgc ctacggcatt gcgccggccg caccactccc acccacatcc
     5461 cgccggtcag gccgactctc cccgctcacg agcgatccgg cgcgccgtgg cgacacgttg
     5521 ccggatcagc tcggcccaca ggtgttcgcc gtgccggtac ccgctcccgg cgaaacgcca
     5581 ctcaccaacc gtcacggtct cccgctcggc aggatcaggc aggccgcgcc gttcccgagc
     5641 ccgctcggcc tggtggtcgg cccgcacccg ccgaagcgcg ctcagggtgg tcgagtagtg
     5701 gcggctcttg gttgagaagt ggccccggta gccgagcatg tgcgcccact gccgcaacgg
     5761 gatcaaccgg taggccggat cgttgccgag cgcgaagcag gtgtagatca tgcgccgggc
     5821 atgctcagtg acgtcgagtc gcgcgacatc gggcacctcc cggataggcc ggtccacggt
     5881 gccggcggac tcggccccct tcgtggcgta cttcgcgatg taggccgcca cacgctgatc
     5941 cgcgatgccc tcaaccgagg tcgcgacata gatggggtcc acgtcgagct gcgtgcccca
     6001 ccggccgacg aacgacgggt catcgtccgg caccggaacc gagaccgcag ccgcggtttc
     6061 ccgaatcgcc tcctggagca cgggcaccgt tgcccagtcc ggcgggcgat cggacggccc
     6121 gtccggtcca tcgaggcgaa tcacggcgtg gaagtggacc aggccccgcg cctgatactc
     6181 ggccaccttt gcgtacgaca gccgaagccg ccgccgcagt tcggcccgcg agatgcccag
     6241 gtgccgggcg aaggtcgccg gtagcgcctg cgtgaagcgc cgccacagct ctcccgcgtg
     6301 cgcgttccac agcaccgcgt cccggtagcc gtagcagtcc acgcagatcg gctgcccgac
     6361 ctgggggtca tcgtcggcat ggcggacccc gcagccttcc ggcctgccgt gcgcgcacac
     6421 cgggttgtcc cgccgaggac gacacggcaa caccttgtca ctcttcaccc ggcgagcgtg
     6481 gaccgcgccg aacgaggggg ccgtgaacgt ggcgaacacg cggggatgag cgctcacgtc
     6541 ctcggggacg cccttgccac cgcgcaggcc ggacaggatc aggtgatagg tgtcgtcccg
     6601 gtagacctcc gagcacgccg ggcaccggga tgcacgacga ttgccacacg cggtcagcag
     6661 atacccggtc ggctcgtcgg ccgtccggta gacgtccagc acttccaacc cggcatcgaa
     6721 ggtgatccgg cgtccctgga gccggatcgg ttgggcacag ccgcccgtcg cccgcaccat
     6781 ctcctgccac cgggcgaagt ccggcatggc ggcgcgctgg gcagcagcga ggacatcggg
     6841 ggagagggga ggaagctcgc tcacgcggca tccctcccgc tcttctcgtc ggcgggatcg
     6901 gtgcggccag gttcgtcggc cagcacggca ccgagaacgg cggccacccg gtgccacttg
     6961 tcggcagacc aacgaggaga ctcggcggct ttgcgtctgc cccactcctg ggcagacatg
     7021 ggggtctgtg tggtcaggtg ggctcctttc ctcgcgggtg gagccaccgc cgcgcatgcg
     7081 gaacagacgc cgagcaccgt gagccagcag ccgaacggcc gaacacgggc acgtcagcga
     7141 gagaacctgg gagtgtgcga ccagaaaatg aaagggcgtg acgcctgccg ctgggccggg
     7201 tccctccagc ggtacggcgt taattatttt gtatgccgcc cggatctcat gcacgccgga
     7261 aaatgggtcg cgtagcttct cgcctgtgtc attccgtcag ggagacggtg acttcttgca
     7321 gttctgttct accccagaag aacgcatatg cgtaccggtc agttccgaag ccggaattgg
     7381 catgacgtct ttgacggcgt cccagggcgt ccgctggcgt ccagcggcgt ccgtagacgt
     7441 ccggctgtgg tgggagactg gtaagggggt tgtcgcctgg cggtaaggct ggaggagcta
     7501 ccgtcgaccg caagacgtcc tggacgtctt gggggttctc gcgtcctcag tcctcacgcc
     7561 agaccagttc aagaaggtcg ggatcgaagc gcctgcgccc cttgccgctc gggtagatga
     7621 tcacggcgtg cagcgcctcc cggatgtagg accgcttcaa cgagagcggt agaccgccct
     7681 cactctccga taggaaccac cgccgccgta tctcggccac gtcggtagcc gagcgggcct
     7741 gtcgtcgttg agctgtcgcc gcgaagctct gcgcgtccgt gcgaagggcg ggatatgcgc
     7801 ttctccagat cgggagccgc cgagaagaac agctcattac tgatattccc tgccgtccat
     7861 tgggcgcgta gctcttcgag ccgagccttg gcgtcttcgt aatcctgttg accaggccac
     7921 tcggtagggc ctgtggaaga ggccaactgc acctcttcga gtttcgcgag gaccgcttcc
     7981 gagacgtaga ggtcaaccat gtccccacgt ctgccgagac cgccacagcc gcccaccgtc
     8041 ttggcgggac agatgtagat gtgctgcacg cagtgcttct ggtgcgtgac ccggagggac
     8101 gcgttgcaca gagcgccgtc tggttgggtc cggccgcacc ggaggaaccc ggtgagcagg
     8161 tggcggggct ctttgaagtc tcgcggaagg atcgggccga gtcgaccgtc ccggtagacg
     8221 aagtgaccct tgcgcgcgtc gaagatggac ttgaccgcca tccactgttc cggcgtcacg
     8281 atcggctgcc actgcccgac caccgggttg ccctcggagt ctcgtatcaa ctcgccgttc
     8341 atctcgcgcc agccgcagac ccgagggttg gtcaatgtga ccttcaggga gcgggaagtc
     8401 cagcggttgc ccagggaagt cttcacgtca ccccgctgcc attggttcac gatggagttg
     8461 agcgatcggc cggcgacgag gtcgaggatc gcctgacgta cgagaggtgc ctcttgcgga
     8521 tcgagagtca agcgatcggg taaccagccg aagggacgcg tgccgccaac gggtagcccc
     8581 tgctcggcgc gtgcccgatg ggagcggcgt atacggcgct gcatcttccg cacctccatc
     8641 ttggagatga ccgcgccgaa cagacccatg ctctcggtgt cttcgctgta gagatccttc
     8701 tgaccccgcg cgtccgcgaa gacgcggccg tcctggtagg tgatcgcttc aacgaagcgt
     8761 tcgtagtcac ccggacgtcg ggcgaggcga tcctcagcca cgatgacgac gcctcgcacc
     8821 tccgggccgt ccggaagctc gcccgagcgg agcaccttca acatggcctc gaactcgtcg
     8881 cggacgacat cggcctttgc agccgacttg tcgttgtccg tgaactcgtg gacgacggtc
     8941 cagccgtacc gggccgcggt ctcccggttg agcttgtgct ggtcctggac tccgtgctcg
     9001 tcccgcttgg tgtcggcgga gatacgagcg taggagacga cgggaatggg gggcgtgtgc
     9061 gttgccaagg cgacctccgc agcaattatt gcccgagcct caggactgtc gccagcggtt
     9121 tccatccgaa attcatcccg ctatggatat tttgggcagg tatgacgtat atgggactgc
     9181 ataaatcagg ccgttatcgt gtccgaaatg cgtggaatcg aatcgcagtg tatggctgcc
     9241 gatgtagtgt ggatgagtga accttcaagc ccagatagaa ctaataacgg ttcctcagga
     9301 tttcacgcgt ctctgtaaca ctgtccttcg agctgagtac ggagatgatt ttcttccaat
     9361 tgatgacgat aggccggata gaggaaatga cggctatctc aagtctgaga aacggatatt
     9421 tgcggcccac tgttttaaaa ggatccaaaa tcgcggtcta gatagtgaaa tatatgccaa
     9481 gatgagcagt gatctgaaaa aggctatcgc cctcaaaggt gatgggggtt gggagattcg
     9541 tcactggacg tttctttcga actatccgat accggaagcc atcgcttccc gcatattgaa
     9601 ggcggggcgt gatgctggta ttgaagttag ctggcgaggg ccggattact ttgctggagt
     9661 gctgcagcgc ttcaagagtg ttagagagct cttcccgaat cttcttgcga atgacatcat
     9721 gtggaagctt gaggagataa ctgagcaaat agaatctctt agagtagatc aaagggatcc
     9781 agtagttact tgggttccgc gaaatcccga agagcagcag ctgttgattt cgcaagctcc
     9841 gccagcttgg gagtacctcc tctttgctgg gtcactcctg caggggaaag agcgactcga
     9901 gggaaaatgg cgcgattttc agatgggata tgggcggcgc aacggcgtat atctcgacga
     9961 taagtctgca gtagctcgcc tcggcggcgt ctttggtgac gcccttgttg tcggtaacgg
    10021 gatcaagcct atcttcgatg aaagagctca ggaggaggct ttcggtcctc ctggagtccc
    10081 tggggatccc gggaagatta tccactttgc cgggcggata atctccactt atgaagatct
    10141 cctagattgg gcaagtgata ttcgcggcac tgtatatcca gaggagatgc gccatgccgc
    10201 ggagcttgct gcccgggtgg cggataggcc ggctagagat atacgggatt ttgttgatcg
    10261 ggtagtggaa
//
