LOCUS       NZ_CP023694            11143 bp    DNA     linear   CON 13-JUL-2024
DEFINITION  Streptomyces coeruleorubidus strain ATCC 13740 chromosome, complete
            genome.
ACCESSION   NZ_CP023694
VERSION     NZ_CP023694.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces coeruleorubidus
  ORGANISM  Streptomyces coeruleorubidus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9335698)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008705135.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:16:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,499
            CDSs (total)                      :: 8,405
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 96 of 229
            Pseudo Genes (incomplete)         :: 175 of 229
            Pseudo Genes (internal stop)      :: 12 of 229
            Pseudo Genes (multiple problems)  :: 53 of 229
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:54:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4791784
            Orig. end                         :: 4802927
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11143
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="15"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11143
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11143
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6143](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6143)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    93..108
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     gene            complement(106..639)
                     /db_xref="GeneID:91418777"
                     /locus_tag="CP976_RS22175"
                     /old_locus_tag="CP976_22190"
     CDS             complement(106..639)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91418777"
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /locus_tag="CP976_RS22175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22190"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_150481937.1"
                     /transl_table=11
                     /translation="MKRTSFDTWPCSIARTADILGDAWSLLVLREVFYGESRFDGFIGS
                     LGIARNTLTDRLRRLEAEGLLRRRAYQSDPVRHEYLLTDKGRDFFGVLAAINAWGDRWL
                     AGDEGAPVVLHHTPCDHDTEARVVCSSCGEQLRHQDMTASTGPGYPARLLDTPAVRDRF
                     APGRPLGDARAGQA"
     misc_feature    647..661
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.57"
                     /tool="antismash"
     misc_feature    complement(656..670)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.1"
                     /tool="antismash"
     gene            complement(698..>1571)
                     /db_xref="GeneID:91418778"
                     /locus_tag="CP976_RS22180"
                     /old_locus_tag="CP976_22195"
                     /pseudo=""
     CDS             complement(698..>1571)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91418778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119462.1"
                     /locus_tag="CP976_RS22180"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="CP976_22195"
                     /product="ABC transporter permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="AASTAVHVLEDGVALIRSEASAATPGPLAATPGPLAATTRLPLAV
                     GEVSGLDDDSVIVNEEWQRHRVGTGSASGSATEHRAPCGSPR"
     gene            1795..2616
                     /db_xref="GeneID:91418779"
                     /locus_tag="CP976_RS22185"
                     /old_locus_tag="CP976_22200"
     CDS             1795..2616
                     /codon_start=1
                     /db_xref="GeneID:91418779"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032569.1"
                     /locus_tag="CP976_RS22185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22200"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_229875455.1"
                     /transl_table=11
                     /translation="MTKQDRAVRTRLALIRSAAEHFEARGYVRASLSEISAGAGVSSGA
                     LHFHFANKAAVAEAVEHAAARALRRVAGRGRGGDGCALQRLVDVTHHVARLLCADVVVR
                     AGLRLNAEAVAGRVRDLRREWRECVHALLAEAERRGELAAGMPPHRSSAVVLAATTGIE
                     VLAREDPGWLARPSLTDLWHLLLPCVAAPHLAGALDPAGTEPEHAGPDIGREPDNGREP
                     DIGRDADLGREPGVGKEADVGNEADASREPDVGRDTGGALEEPGRAGLALR"
     misc_feature    complement(2705..2725)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.88"
                     /tool="antismash"
     gene            2784..4124
                     /db_xref="GeneID:91418780"
                     /locus_tag="CP976_RS22190"
                     /old_locus_tag="CP976_22205"
     CDS             2784..4124
                     /codon_start=1
                     /db_xref="GeneID:91418780"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032564.1"
                     /locus_tag="CP976_RS22190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22205"
                     /product="cytochrome P450"
                     /protein_id="WP_150481938.1"
                     /sec_met_domain="p450 (E-value: 4.4e-18, bitscore: 64.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQQACPFLAIDPSGQDLYDEIARIRAQGPAVEVELPGGVRAWWI
                     NGLELNKRLLAGPETSKDAFQHWPAWINGDISRTWPLAIWVSVRNMVTAYGTDHSRLRK
                     PMSAAFTKRRVDALLPRVQEIVDRALDGLERIPEGEVIDLRAGFAAPIPHEVVCELFGV
                     PPGEDGPRGALYRIINRFFDTAISLEDAQANGVELYGTLTAFLQYKRDHPADDLTTALM
                     AAHDEGSLSEQELMDNLILLLTAGFETTVNLIDNTVHSLLAHPDQLELVRSGQATWEDA
                     IEESLRFEAPGAMSGLRYAVEDIEIEPGLTIPKGDPVVVSFAGAGRDPERHGPDAERFD
                     VTRSTSRDHVSFGHGVHHCLGRPLAMAEATVALTSLFDRFPRLALADPARPPKRLRSII
                     STGHQELPVLLHGRDGGPVPGPRAEEAEAIRRLSGLGGTSGTRVPRV"
     gene            complement(4069..5004)
                     /db_xref="GeneID:91418781"
                     /locus_tag="CP976_RS22195"
                     /old_locus_tag="CP976_22210"
     CDS             complement(4069..5004)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91418781"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032561.1"
                     /locus_tag="CP976_RS22195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22210"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_150481939.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.2e-10, bitscore:
                     39.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVDFLITGGSGFVGSRVAATAHRQPGVRVRHLSRRTPPPGAEPG
                     ASVHGDLADPLSLRGTCAGADVLVHCATRVGGDAETVEAVNELGTRALVEEAVRAGVPR
                     IVYLSTAAVYGRGPFRGVRPGEVPIAPASATSRTRAAAERHVLDAGGLVLRPHLVYGEG
                     DRWVVPGLVWLLGELSATLTGCEALQSMIDVDTLGRAVVAAALSPTHDGGVHHVNHPEP
                     VGASDLLGAAGELLGEPGGGAAVDVATALDRAAGKPLALHHLGMLTVDHWFVDDAFWKD
                     LDCSPGEGFAAGFRRAAPWYRSFLRGRKAS"
     gene            complement(5001..6143)
                     /db_xref="GeneID:91418782"
                     /locus_tag="CP976_RS22200"
                     /old_locus_tag="CP976_22215"
     CDS             complement(5001..6143)
                     /codon_start=1
                     /db_xref="GeneID:91418782"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032560.1"
                     /locus_tag="CP976_RS22200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22215"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_150481940.1"
                     /sec_met_domain="AfsA (E-value: 6e-21, bitscore: 73.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQSEPLSAPTDDQTGQGGRPANRSTAAVENAPQATVIDPVDPPE
                     AAAAIEPAQPTEPTSVTKELAHRTVEADVFPTQWRRISDTRFRFTAHWPATHPFFGPVD
                     DHHQDPMIVGETLRQASMVLAHAEFGAPADTHFVMWDLTVHTDPFALLLSDAAEPVDVD
                     VVCSEIRRRGRGLHSMRTTMEFRRAGRFIARGTGSTGCTSPLAYRRLRQKQLAALGTPV
                     PLLPGISPALAGRSRAEDVVLAPSDRPGVWRLRVDTGHPVLFPRPNDHVPGMVLFEAAR
                     QAAAAASGRHTFLPRSMTATFARYAELHSPCWIETEVLDENPGIPGDPGDPGDPGDPGD
                     PGDPGDPGETTVRVSGRQDGESVFSATLTCAQPARVRSLS"
     misc_feature    complement(6123..6125)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6206..6219)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.06"
                     /tool="antismash"
     misc_feature    complement(6207..6220)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.63"
                     /tool="antismash"
     misc_feature    complement(6208..6221)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.64"
                     /tool="antismash"
     misc_feature    6208..6222
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.42"
                     /tool="antismash"
     misc_feature    6279..6292
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            6303..7001
                     /db_xref="GeneID:91418783"
                     /locus_tag="CP976_RS22205"
                     /old_locus_tag="CP976_22220"
     CDS             6303..7001
                     /codon_start=1
                     /db_xref="GeneID:91418783"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032559.1"
                     /locus_tag="CP976_RS22205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22220"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_150481941.1"
                     /transl_table=11
                     /translation="MARQERAIRTRRTILEAAAAVFDERGYTSATIGEILDRAGVTKGA
                     LYFHFGSKEELAVGVFEEQLAITLPPQSSKLQELIDSGLVLARRLRSEPLVRASVGLAL
                     DQGAMELDRTPAFRMWFDQNVQRLGEAKAQGELLPHVDVAETAELLVGSFSGVQLLSQL
                     RCQRQDLERRVVVMFEHFLPSIAVPAVLARLDISVERAERVLAQVQQRPGRERMETQEG
                     AVPAQVSSGA"
     gene            7136..8569
                     /db_xref="GeneID:91418784"
                     /locus_tag="CP976_RS22210"
                     /old_locus_tag="CP976_22225"
     CDS             7136..8569
                     /codon_start=1
                     /db_xref="GeneID:91418784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032556.1"
                     /locus_tag="CP976_RS22210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22225"
                     /product="DUF2252 domain-containing protein"
                     /protein_id="WP_150481942.1"
                     /transl_table=11
                     /translation="MTRDSAGAEGGAVHGETRRLPRVRGFAEWPAEGSPKDEGKALRKR
                     VPRRAHATLDLDASRPDAVSAVEESGRGRLPELTPIRVGRMAATPFAFLRGAAGLMAYD
                     LARTPMTRIKAQICGDAHAANFGLYGDARGGLVIDLNDFDETVLGPWEWDVKRLAASLV
                     LAGREAGADEERCRKAAYDAVGAYRRTMRLLARLPVLDAWNAIADEELVSHTDAHDLLG
                     TLERVSEKARHNTSGRFAARSTEATEDGGRRFVDAPPVLRRVPDAEAAAVAASLEEYVG
                     TLPEDRLPLLARHAVHDVAFRVVGTGSVGTRSYVVLLLDHRGEPLVLQVKEARASALVP
                     HLATAGFDAPVVDHEGRRVVLGQKRMQVVSDILLGWTTVDGHPFQVRQFRNRKGSVDPA
                     ALAADQIDDYGRMTGALLARAHAHSADPRLVAGYCGKSEELDEAVADFAVAYADRTEAD
                     HADLVAGVRAGKVAAEMGV"
     gene            8641..9552
                     /db_xref="GeneID:91418785"
                     /locus_tag="CP976_RS22215"
                     /old_locus_tag="CP976_22230"
     CDS             8641..9552
                     /codon_start=1
                     /db_xref="GeneID:91418785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437092.1"
                     /locus_tag="CP976_RS22215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22230"
                     /product="hypothetical protein"
                     /protein_id="WP_190146861.1"
                     /transl_table=11
                     /translation="MTTPEAEQGGGGAGEERVTPEGDAARAEGGEDRPEGGAAPTESEA
                     ARPEARLERAVRAAEQALIEYEIAVETFRVEIENFSRLHEQKLGPLYGRLEELDARILE
                     ARAARSGDPEDRRRAEEARARLAPIPGVEELLHGWMDDSGLFPEAVAMLTDQAVRPPQR
                     VRPSEEARKLYRELARKAHPDLAQEEKERERREEFITRVNAAYARGDEAELRELAEEWA
                     KGPELKRGPSRAEELYARLEWLAQRKELLALLAKELEESAIGHMLQLAPDDPDGLLDEI
                     GQQLLTQVTERERELAALLAEG"
     misc_feature    complement(8681..8696)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     gene            9611..9940
                     /db_xref="GeneID:91418786"
                     /locus_tag="CP976_RS22220"
                     /old_locus_tag="CP976_22235"
     CDS             9611..9940
                     /codon_start=1
                     /db_xref="GeneID:91418786"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019055193.1"
                     /locus_tag="CP976_RS22220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_22235"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_020273126.1"
                     /transl_table=11
                     /translation="MPTVEVADLKDGDFLLDVREDDEWKAGHAEGALHIPISDFVARYG
                     ELTEAAPQDGRVHVICRSGGRSAQVTMYLAQQGIDAVNVDGGMQVWEAAGRPVVTDDGQ
                     PGFVL"
ORIGIN
        1 accgtcggaa cgggcggcat cgatcagatg ccgcccgttt ctcgtgtctg cggatgtgat
       61 ggttgtctcg cggcatccgg gggttgtgct ggtgtgagga agctctcagg cctggccggc
      121 tcgggcatcc cccagcggcc ggccgggggc gaagcggtcc cgtacggcgg gggtgtcgag
      181 gaggcgggcc gggtagccgg ggccggtgct ggcggtcatg tcctggtggc gcagttgctc
      241 cccgcaggac gagcagacga cccgggcctc ggtgtcgtgg tcgcagggcg tgtggtgcag
      301 gaccaccggg gcgccctcgt cgccggccag ccagcggtcg ccccaggcgt tgatcgcggc
      361 gagtacgccg aagaagtcgc ggcccttgtc cgtcagcagg tactcgtggc ggaccgggtc
      421 gctctggtag gcccgccgcc gcagcaggcc ctcggcctcc aggcgccgca gccggtcggt
      481 gagggtgttg cgggcgatgc cgagcgagcc gatgaagccg tcgaagcggg attccccgta
      541 gaagacctcg cgcagcacca gcaggctcca ggcgtcgccc aggatgtcgg cggtgcgggc
      601 gatggagcag ggccaggtgt cgaacgaggt ccgtttcacg gctgccaggc tatcggtttc
      661 atcatgagat ggaccggcct cggcgtccct gtgcccgtca ctcccgtatg cccgccagtt
      721 ccgccgggcg gcggcgcagg gcgagggcgg ccggggcgaa tgcggaggtc gtcgcgagga
      781 gggcgcagac ggctgtggtc gtgccgaggg cctgccacgg gagctcgatc gtggccgggg
      841 ccgacaacag ggcgagggcg ctcgccctgt cggccaggtt aaaggcgcga ccaggaggcc
      901 gaggagggcg ccggccgcca cgaccgtcag cgcctcggcg ccggtcagcc gcaggacctg
      961 ccaccgggtg gctccggcca gccgcagtag ggacaggtcg cgggcccggt cggacgtcgc
     1021 catgaccatg gtgttggcca cggagatgcc cgtgtagagg agggcgatgc cgaggaccag
     1081 gaggaggccg aggcgggtgg tgcggttcgt ctcggggtgg gtggcgcgga tccatgcgtc
     1141 gcgggtgagg atctgcccgt tcccggggcc gagcgcccgg tgcggggcgg tggcgacggt
     1201 gttcgggtcc gcgtcgtcgg tcagggccac gtcgatccgg tcgaccgtgg cgccgggggc
     1261 gttgcgtggg gtgacgtaga cgccgttgtc gccggtgccg gtggtcatca ccgcggcgat
     1321 ccgcagggtg cgcggtgttc cgtcgccgag ccagacgcgg acccagtgcc cacccggtgc
     1381 cgctgccact cctcgttgac gatgaccgag tcgtcgtcga ggccgctcac ctcgccgacg
     1441 gccagcggga ggcgggtggt ggccgcgagg gggccggggg tggcggcgag ggggccgggg
     1501 gtggcggcgc tggcctcgga gcggatgagg gcgacgccgt cctccaggac gtggacggcc
     1561 gtcgaggccg ccggcggacg agacccatcg ccgggtgctg gccgtgctgg cgtatctgcg
     1621 ggcgtaggcg cgcggtcggg cctggcgggt cggacccggc cggacacctt cgtccccgcg
     1681 atggaacgac gccgtcaccg tgaagcccaa cgccttccgc gcatacgcgc gttgtggaaa
     1741 aaccagcgag ttggtttcat tgcgtcgcca tcagagccct ccacgggagc cagggtgacc
     1801 aaacaggacc gggccgtccg gacccgtctc gcgctgatcc ggtcggcggc cgagcacttc
     1861 gaggcacggg gatacgtccg ggccagtctg tcggagatca gcgccggagc cggtgtcagc
     1921 tccggcgctc tgcacttcca cttcgcgaac aaggccgccg tggccgaggc cgtcgagcac
     1981 gcggccgccc gtgccctgcg ccgggtcgcg ggccggggcc gcggcggtga cggttgcgcc
     2041 ctccagcgcc tcgtggacgt cacgcaccac gtcgcccggc tgctgtgcgc cgacgtcgtc
     2101 gtgcgcgcgg ggctgcggct caacgccgag gcggtcgccg ggcgggtgcg ggacctgcgc
     2161 cgggagtggc gggagtgcgt gcacgcgctg ctggccgagg cggagcgcag gggcgaactc
     2221 gccgccggga tgccgccgca ccgttccagc gccgtcgttc tcgccgccac gaccggcatc
     2281 gaggtgctgg cccgggagga ccccggctgg ctcgcccggc cctccctgac cgacctgtgg
     2341 cacctgctcc tgccgtgcgt ggccgccccg cacctggccg gcgcgctgga cccggccggg
     2401 acggagccgg agcatgcggg gcccgacatc ggcagggagc cggacaacgg cagggagccg
     2461 gacatcggca gggacgcgga cctcggcagg gagccgggcg tcggcaagga ggcggacgtc
     2521 ggcaacgagg cggacgccag ccgggagccg gacgtcggca gggacaccgg cggggcgttg
     2581 gaggagccgg gccgggcagg gctcgcgctg cggtagcggg gtgtcccggt cgactttcgg
     2641 tcggcttttc tgacggcttc cggtcggcct tcccatggcc ggcagtcaat gaagtggtgg
     2701 ccaaaacaaa ccggttgcca agttttttcg tcccttcgat cgcacgatgg ttccccagcc
     2761 tctggaccaa ggagcgatga gtcatggcac agcaggcctg ccccttcctg gcgatcgacc
     2821 cgtccggcca ggacctctac gacgagatag cccggatccg ggcccaggga cccgccgtgg
     2881 aggtcgaact cccgggcggc gtacgggcgt ggtggatcaa cggcctggag ctgaacaagc
     2941 ggctcctggc cggacccgag acgagcaagg acgccttcca gcactggccg gcctggatca
     3001 acggggacat ctcccgcacc tggccgctcg cgatctgggt ctcggtgcgc aacatggtca
     3061 cggcctacgg caccgaccac agcaggctgc gcaagccgat gtccgccgcc ttcaccaagc
     3121 gccgcgtcga cgcgctgctc ccgcgcgtcc aggagatcgt cgaccgggcg ctggacggcc
     3181 tggagcggat ccccgagggc gaagtcatcg acctgcgggc ggggttcgcg gcgcctatac
     3241 cgcacgaggt ggtgtgcgag ctcttcggcg taccgccggg tgaggacggt ccgcgagggg
     3301 ccctctaccg catcatcaac cgcttcttcg acacggcgat ctcactggag gacgcccagg
     3361 ccaacggggt cgagctgtac ggcacgctga ccgcgttcct ccagtacaag cgcgatcacc
     3421 cggccgacga cctgacgacg gccctgatgg ccgcgcacga cgaaggcagc ctcagcgaac
     3481 aggagttgat ggacaacctg atcctgctgc tcaccgcggg cttcgagacg accgtcaacc
     3541 tcatcgacaa caccgtgcac agcctgctcg cccaccccga ccagctcgaa ctcgtccgct
     3601 ccggacaggc gacctgggag gacgccatcg aggagtcact gcgcttcgag gcgcccggtg
     3661 ccatgtcggg tctgcgctac gccgtcgagg acatcgagat cgagccaggc ctgacgatcc
     3721 cgaagggtga tcccgtcgtg gtctccttcg ccggtgccgg acgcgacccc gagcggcacg
     3781 gcccggacgc cgaacggttc gacgtcaccc ggtccaccag ccgcgaccac gtctccttcg
     3841 gccacggcgt gcaccactgc ctgggccggc ccctggccat ggccgaggcg acggtggccc
     3901 tgacgtcctt gttcgaccgg ttcccgcggc tggccctggc cgatccggcc cggcctccga
     3961 agcggctgcg ctccatcatc tccacggggc accaggagtt gcccgtgctg ctgcacggcc
     4021 gcgatggcgg gccggtaccg ggcccgcgtg cggaggaggc ggaggcgatc aggaggcttt
     4081 ccggcctcgg aggaacgagc ggtaccaggg tgccgcgcgt ctgaatccgg ccgcgaatcc
     4141 ctcgccgggg gagcagtcga ggtccttcca gaaggcgtcg tccacgaacc agtggtccac
     4201 cgtgagcatg ccgaggtgat ggagggcgag cggcttcccg gcggcccggt ccagggcggt
     4261 ggcgacgtcc accgccgcac cgccgccggg ctcccccagc agttcgcccg ccgccccgag
     4321 caggtccgag gcccccacgg gctcggggtg gttgacgtgg tgcacaccgc cgtcgtgcgt
     4381 aggggacagg gccgcggcca ccaccgcacg gcccagggtg tccacgtcga tcatcgactg
     4441 gagcgcctcg cagccggtca gggtcgccga caactcgccc agcagccaca ccagtccggg
     4501 gacgacccac cggtcgccct cgccgtacac gaggtgcggg cgcagcacca ggccgcccgc
     4561 gtcgaggacg tgccgttcgg cggcggcccg ggtgcggctg gtggccgacg ccggggcgat
     4621 cggcacttcc ccggggcgca ctccgcggaa ggggccccgg ccgtacacgg cggctgtgct
     4681 caggtacacg atccggggca ccccggcgcg cacggcctcc tcgaccaggg cccgcgtgcc
     4741 cagctcgttg acggcctcga cggtctcggc gtcgccgccg acccgtgtgg cgcagtgcac
     4801 caggacgtcg gcgcccgcgc aggtgccgcg cagggagagc gggtcggcga ggtcgccgtg
     4861 gacggacgcg ccgggctcgg ctcccggtgg tggggtacgg cgtgacaagt gccgtacgcg
     4921 gacaccaggt tgccggtgtg cggtggccgc gaccctgctg ccgacgaagc cgctcccgcc
     4981 cgtgatcaga aaatcgaccg tcacgacagc gaccgtaccc gggccggctg cgcgcaggtc
     5041 agcgtggcgc tgaagacgga ctcgccgtcc tgccgcccgg acacccgtac ggtcgtctct
     5101 cccggatctc ccggatctcc cggatctccc ggatctcccg gatctcccgg atctcccgga
     5161 tctcccggga ttcccgggtt ctcgtcgagc acctccgtct cgatccagca cgggctgtgc
     5221 agctcggcgt accgggcgaa cgtcgccgtc atcgagcggg gcaggaaggt gtgccggccg
     5281 gacgcggcgg ccgccgcctg ccgggccgcc tcgaacagga ccatgccggg cacgtggtcg
     5341 ttgggccgcg ggaagagcac ggggtggccg gtgtccacgc gcagccgcca gacgccggga
     5401 cggtcggagg gggcgaggac gacgtcctcg gcgcgggagc ggccggcgag cgccggggat
     5461 atgcccggca gcagcggcac cggtgtgccc agtgcggcca gctgcttctg ccgcagacgg
     5521 cggtaggcga gcggggaggt gcagccggtg ctgccggtgc cgcgggcgat gaagcgtccc
     5581 gcacggcgga actccatggt ggtccgcatg ctgtgcagac cgcgaccgcg gcggcgtatc
     5641 tcggaacaga cgacgtccac gtcgaccggc tcggcggcgt ccgacagcag gagggcgaac
     5701 gggtcggtgt ggaccgtcag gtcccacatg acgaagtggg tgtcggcggg ggctccgaac
     5761 tcggcgtgcg cgagcaccat cgaggcctgc cgcaacgttt cgcccacgat catcggatcc
     5821 tggtggtggt cgtccacggg gccgaagaag ggatgggtgg ccggccagtg agcggtgaag
     5881 cggaagcgcg tatcggagat acggcgccac tgggtgggga agacatctgc ttctacggtg
     5941 cggtgggcga gttccttggt gaccgaggtg ggttcggtgg gttgcgcggg ttcgatggct
     6001 gcggcggctt cggggggatc gacgggatcg atgactgtgg cttgtggcgc gttctcgacg
     6061 gccgcggtgg atctgtttgc cggacggccc ccctgtccgg tctgatcgtc cgtgggtgcg
     6121 cttaaaggtt ctgactgcgg catgagaacc cccaggatcg gcagcctggc tgaggtcggc
     6181 gtttgactaa gatacggacg agccggtttt tttcaaaggg ttggtcaaac cgctgggaca
     6241 ggctcccgcg cccccgccgc acagcgattt cacaggaatc gacggagatc aggaggcacc
     6301 cgatggcgcg acaggagcgc gcgatccgca cccggcggac catcctcgaa gcggccgcgg
     6361 ccgtcttcga cgaacgcggc tacacctcgg cgaccatcgg ggagatcctg gaccgggcgg
     6421 gcgtcaccaa gggcgcgctg tacttccact tcggttccaa ggaggagctt gcggtggggg
     6481 tgttcgagga gcagctcgcg atcacgctcc cgccgcagtc cagcaagctc caggaactca
     6541 tcgactccgg gctggtgctg gcccgccggc tgcgctccga accgctggtt cgcgccagtg
     6601 tggggctcgc cctcgaccag ggggccatgg agctcgaccg cacgccggcc ttccgtatgt
     6661 ggttcgacca gaacgtacag cggctgggtg aggcgaaggc gcagggcgaa ctgctgccgc
     6721 atgtcgacgt ggcggagacg gcggaactgc tggtcggcag cttctcgggg gtgcagctgc
     6781 tgtcccagct acggtgccag cggcaggacc tggagcgccg cgtcgtcgtg atgttcgaac
     6841 acttcctgcc gagcatcgcc gtccccgcgg tgctggcccg gctcgacatc tcggtggagc
     6901 gggctgagcg ggtgctggcc caggtgcagc agagacccgg gcgggagcgg atggagacgc
     6961 aggagggggc tgtgccggcg caggtctcgt cgggggctta ggagtcgcct ttcgccgggt
     7021 gcccgcacgg gttgtccaca gctgtggagt tgtccacagg gtctgacgcg tttcggccac
     7081 cggctgtacg gtcgcacgag ttgatgttcg ttcgcgtgcg ggggaggcgg tcggtgtgac
     7141 cagggacagt gctggggccg agggcggggc ggtgcatggg gagacccgtc ggctgccccg
     7201 ggtgcggggt ttcgccgagt ggcccgccga gggttcgccc aaggacgagg gcaaggccct
     7261 gcgcaagcgg gtcccacgcc gcgcgcacgc cacgctcgac ctggacgcgt cccggcccga
     7321 cgcggtgagc gcggtcgagg agtcgggccg cggccggctc cccgagctca caccgatccg
     7381 ggtcggccgc atggccgcca caccgttcgc gttcctgcgt ggcgccgccg gcctcatggc
     7441 ctacgacctg gcgcgcaccc ccatgacccg gatcaaggcg cagatctgcg gcgacgccca
     7501 cgcggccaat ttcggcctgt acggcgacgc gcgcggcggc ctggtcatcg atctgaacga
     7561 cttcgacgag acggtgctcg gcccctggga gtgggacgtg aagcgcctcg cggcctccct
     7621 ggtgctcgcg ggccgggagg ccggagcgga cgaggagagg tgccgcaagg cggcctacga
     7681 cgcggtgggc gcctaccgcc gcaccatgcg gctgctggcc aggctcccgg tgctggacgc
     7741 gtggaacgcc atcgcggacg aggaactcgt ctcccacacg gacgcccacg acctgctcgg
     7801 cacgctggag cgggtctccg agaaggcccg ccacaacacc agcggccgct tcgcggccag
     7861 gtcgacggag gccacggagg acggcgggcg ccgcttcgtc gacgccccgc cggtgctgcg
     7921 gcgcgtcccg gacgcggagg ccgcggcggt ggcggcgtcc ctggaggagt acgtcggcac
     7981 gctgccggag gaccgcctgc cgctcctcgc ccggcacgcg gtgcacgacg tggcgttccg
     8041 cgtcgtcggc accggcagtg tcggcacccg gtcctacgtc gtgctgctcc tggaccaccg
     8101 gggtgagccg ctggtgctcc aggtcaagga ggcgcgggcg tcggccctgg tgccgcatct
     8161 ggcgacggcc ggtttcgacg ctccggtggt cgaccacgag ggccggcggg tcgtgctggg
     8221 gcagaagcgg atgcaggtcg tcagcgacat cctgctcggc tggacaacgg tcgacgggca
     8281 tcccttccag gtccgccagt tccgcaaccg caagggcagc gtcgacccgg ccgccctggc
     8341 cgccgaccag atagacgact acggccggat gaccggcgcc ctcctggccc gcgcccacgc
     8401 ccacagcgcc gacccgcgcc tcgtcgccgg ctactgcggc aagagcgagg agctggacga
     8461 ggcggtggcg gatttcgccg tcgcctatgc ggaccgtacg gaggcggacc atgcggacct
     8521 ggtggcgggg gtgcgggcgg ggaaggtcgc cgcggagatg ggcgtctgaa gcctgcggga
     8581 catcgggcgt gcggtcgtcc ggagcacggg agcggtccgg ccgtggccta cgctggtcgg
     8641 gtgacgaccc cggaagctga gcagggtggt ggcggcgccg gtgaggaacg ggtgacgccc
     8701 gaaggtgacg cggcgcgtgc cgagggcggc gaggaccgcc ccgaaggcgg cgcggcgcct
     8761 accgaaagcg aggcggcgcg gcccgaggcg cggctggagc gggccgtgcg ggccgccgag
     8821 caggcgctga tcgagtacga gatcgcggtg gagaccttcc gcgtcgagat cgagaacttc
     8881 tcccggctgc acgaacagaa actcggtccg ctctacggcc ggctcgagga gctggacgcc
     8941 cggatcctcg aggccagggc cgcccgcagc ggtgacccgg aggaccggcg ccgggcggag
     9001 gaggccaggg cccggctcgc gccgatcccc ggcgtcgagg agctgctgca cggctggatg
     9061 gacgacagcg gcctgttccc ggaggccgtg gcgatgctca cggaccaggc ggtccggcca
     9121 ccgcagcggg tccgccccag cgaggaggcc cgcaagctct accgcgagct cgcccgcaag
     9181 gcccacccgg acttggcgca ggaggagaag gaacgcgagc ggcgcgagga gttcatcacc
     9241 cgggtgaacg ccgcctacgc ccggggcgac gaggcggagc tgcgcgaact cgccgaggag
     9301 tgggccaagg ggcccgagct gaagcggggc ccgagccgcg ccgaggagct ctacgcccgc
     9361 ctcgaatggc tcgcccagcg caaggaactg ctcgccctgc tcgccaagga gctggaggag
     9421 agcgccatag gccacatgct ccagcttgcc ccggacgacc cggacggcct cctggacgag
     9481 atcgggcagc agctgctcac gcaggtcacc gagcgggagc gggagctggc cgcgctgctg
     9541 gcggagggct gagccgcggc ccggccgggc cggttcgggt agcgtcggac gcatgagttt
     9601 tggagctggt gtgcccacgg tcgaggtcgc ggacctcaag gacggcgact tcctgctgga
     9661 cgtccgggag gacgacgagt ggaaggccgg tcacgccgaa ggggcgctgc acatccccat
     9721 cagcgatttc gtcgcccggt acggggagct gaccgaggcc gccccgcagg acggccgggt
     9781 gcatgtgatc tgccgctccg gcgggcgttc cgcccaggtc acgatgtacc tggcccagca
     9841 gggcatcgac gccgtgaacg tcgacggcgg catgcaggtg tgggaggcgg cgggccgtcc
     9901 cgtcgtgacg gacgacgggc agcccgggtt cgtcctgtag cggctgtagc ggctgtagcg
     9961 gctgtagcgg ctgtagcggc tgtagcggct gtagcggctg tagcggcctg gcgcccgccc
    10021 gtggcgcccg cccgccggtg ggatcaggcc aacgggtggg cggccagcag gtcgcccagg
    10081 gcctcctcgt gcgccgccgc cgggccgagt gacagctcca ggtgcttggc ccaggcgtgg
    10141 taccggtgca ggggatagtc gacgtcggcg ccgaacccgc cgtgcagatg ctgcgccgtc
    10201 tgcacgaccc ggcgtacgcc ctccgccgcc cagatcttgg cgacggcgac gtcaccggct
    10261 gcgggcagcg cgcccggcgc ccccgaggcg atccgccacg cggcctgcca cagtgtggcc
    10321 tccatcgcgc gcagatcgat gtagcggtcg gcggcctgca cggcgacggc ctggaaggtc
    10381 gcgatcggga agccgaactg ctcccgctcg ctcgtgtact ccccggtcat ccgcagcacg
    10441 cgttccccga gcccgagcgc cagcgcacac gtcccggtgg ccagcaggtc acgcagccac
    10501 tcccacgcgc cctcggcgtc gatcacgtcc cgggcggcca gccgcgccga ctccagccgc
    10561 agctcgccga gccgctcacc gctcgtggag aactgctcgg ccaggacggc cccctcgtgc
    10621 ccccgcggga ccagggccag cacggtccgc ccggtcgccg tgtgcgcggg gacgaccgtg
    10681 acgtccgcgt cgtacgccca gggcaccgcc gtctgcactc cgtccagcac ccacgccgcg
    10741 ccgtcctgcc gtgcggtcac ggcgagctcg gccgggtcgt ggccggtgcg gccgtgcgcg
    10801 gcgacggtca gcacgagctc gccccggccc gcccggggca gcagcgccga cttcgcctcc
    10861 tcgccgccga aggcctggac ggcggcggct gccgcgctgc tctccagcag cggcaccctg
    10921 gccagcaccc gcgccgactc ccgcagcacc aggcacagcg cgatcgcgtc cagcccggcc
    10981 ccgccgtacc gggaatccag cagcagactc agcaggtcgg cgtcggcgag tttcgcccac
    11041 agggcgcggt cgcaggcgtc ggcgacggcg ccgggggtga gggccggact gggcacggcg
    11101 tcgggcgcga ccccggcgaa cacgcccttc gccgcctcgg ccg
//
