LOCUS       NZ_CP023694            10504 bp    DNA     linear   CON 13-JUL-2024
DEFINITION  Streptomyces coeruleorubidus strain ATCC 13740 chromosome, complete
            genome.
ACCESSION   NZ_CP023694
VERSION     NZ_CP023694.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces coeruleorubidus
  ORGANISM  Streptomyces coeruleorubidus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9335698)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008705135.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:16:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,499
            CDSs (total)                      :: 8,405
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 96 of 229
            Pseudo Genes (incomplete)         :: 175 of 229
            Pseudo Genes (internal stop)      :: 12 of 229
            Pseudo Genes (multiple problems)  :: 53 of 229
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:54:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3414035
            Orig. end                         :: 3424539
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10504
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="12"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10504
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10504
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5504](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5504)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     tRNA            336..408
                     /anticodon=(pos:3414404..3414406,aa:Arg,seq:tct)
                     /db_xref="GeneID:91417517"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="CP976_RS15680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="CP976_15720"
                     /product="tRNA-Arg"
     gene            336..408
                     /db_xref="GeneID:91417517"
                     /locus_tag="CP976_RS15680"
                     /old_locus_tag="CP976_15720"
     gene            567..830
                     /db_xref="GeneID:91417518"
                     /locus_tag="CP976_RS15685"
                     /old_locus_tag="CP976_15725"
     CDS             567..830
                     /codon_start=1
                     /db_xref="GeneID:91417518"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP976_RS15685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CP976_15725"
                     /product="hypothetical protein"
                     /protein_id="WP_167537346.1"
                     /transl_table=11
                     /translation="MVSSAGPGIAPGYAERAARLPGVAAASGQLSVDGELSRADADQDD
                     DASVIGGNLVVMRADAVPDLLDLGVHTGSLARLGETSVALAW"
     gene            824..1252
                     /db_xref="GeneID:91417519"
                     /locus_tag="CP976_RS15690"
                     /old_locus_tag="CP976_15730"
     CDS             824..1252
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91417519"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP976_RS15690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CP976_15730"
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_150480920.1"
                     /transl_table=11
                     /translation="MVTAGRRGKFALLRLAGAQSAQVLWMPAWEAVLTCAIALLPAALV
                     CAAVLVPLSIAVTGSAVPALAGGPLAALAAGAPLVAVATTTLFGRSAMRSAAATPGGLA
                     RAVSLTPPPCPEGPTPLPAARLRPDHGPGNPSWAEEGV"
     gene            1254..2213
                     /db_xref="GeneID:91417520"
                     /locus_tag="CP976_RS15695"
                     /old_locus_tag="CP976_15735"
     CDS             1254..2213
                     /codon_start=1
                     /db_xref="GeneID:91417520"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="CP976_RS15695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15735"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_150480921.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1e-51, bitscore:
                     173.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQRVATEVADWSPVADEVEVVSFDTHMDGEDALAA
                     ALADFDIVVTLRERVPFPGSLIARLPRLKLIVASGLRNSVIDYAAAEEHGVTVCGTTSS
                     STPPVELTWALLLGLARGLVEESNALRSGGPWQSTVGADLYGSRLGLLGLGKIGSRVAR
                     VGLAFGMHVSAWSQNLTKERTDEIGVELAPSREDLLAGSDFVSIHLALSDRTRGLLGPA
                     ELALLKPTAYLINTSRAAIVDQEALLAALHEGRIAGAGVDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSQANYATYYGQAVENIRAFLSGAPVRRLP"
     gene            complement(2365..2640)
                     /db_xref="GeneID:91417521"
                     /locus_tag="CP976_RS15700"
                     /old_locus_tag="CP976_15740"
     CDS             complement(2365..2640)
                     /codon_start=1
                     /db_xref="GeneID:91417521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987898.1"
                     /locus_tag="CP976_RS15700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15740"
                     /product="hypothetical protein"
                     /protein_id="WP_150480922.1"
                     /transl_table=11
                     /translation="MPNAKRIAVVAGVLWSVALLGAGAGQAFALDGSGGSSGSGKCVDD
                     GKGNVRCVHEAVYRDGDGNVHVVASQAQNCSSEGCNSSVVIGGKES"
     gene            complement(2773..3039)
                     /db_xref="GeneID:91417522"
                     /locus_tag="CP976_RS15705"
                     /old_locus_tag="CP976_15745"
     CDS             complement(2773..3039)
                     /codon_start=1
                     /db_xref="GeneID:91417522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990343.1"
                     /locus_tag="CP976_RS15705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15745"
                     /product="hypothetical protein"
                     /protein_id="WP_150480923.1"
                     /transl_table=11
                     /translation="MRKFQKAAVVMAALGTVTFLGAGVSQAGDGPALEVDSKQSNTCGN
                     ESQSGVLNIGRINVQILDILANQPVDQSKSVTCTNAFALGGGH"
     misc_feature    3144..3160
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 18.22"
                     /tool="antismash"
     gene            complement(3281..3541)
                     /db_xref="GeneID:91417523"
                     /locus_tag="CP976_RS15710"
                     /old_locus_tag="CP976_15750"
     CDS             complement(3281..3541)
                     /codon_start=1
                     /db_xref="GeneID:91417523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826204.1"
                     /locus_tag="CP976_RS15710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15750"
                     /product="hypothetical protein"
                     /protein_id="WP_150480924.1"
                     /transl_table=11
                     /translation="MRKYQKAAVVAVMLGSVSFLGAGVSQAGDKPAFEVDSKQSNTCGN
                     ESQSGVLNIGRANVQVLNILANQPVDQSTSVPCSNAFGIGG"
     misc_feature    complement(3573..3588)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     misc_feature    complement(3592..3606)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.6"
                     /tool="antismash"
     misc_feature    3605..3620
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.61"
                     /tool="antismash"
     misc_feature    3609..3618
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.81"
                     /tool="antismash"
     misc_feature    3651..3664
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.0"
                     /tool="antismash"
     misc_feature    complement(3651..3665)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.19"
                     /tool="antismash"
     misc_feature    3652..3665
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.9"
                     /tool="antismash"
     misc_feature    complement(3652..3666)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.04"
                     /tool="antismash"
     misc_feature    3653..3666
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     misc_feature    complement(3654..3667)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.47"
                     /tool="antismash"
     gene            3770..4081
                     /db_xref="GeneID:91417524"
                     /locus_tag="CP976_RS15715"
                     /old_locus_tag="CP976_15755"
     CDS             3770..4081
                     /codon_start=1
                     /db_xref="GeneID:91417524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990346.1"
                     /locus_tag="CP976_RS15715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15755"
                     /product="chaplin"
                     /protein_id="WP_150480925.1"
                     /transl_table=11
                     /translation="MSRIAKVGAVALGTGAMVLSGAGLAAADADAKAAAIGSPGFLSGN
                     VVQVPIHVPINVCGNTVTVIGALNESAGVCKNGNDEKDKKGKKDNKKADKKALHHHGR"
     gene            complement(4151..5017)
                     /db_xref="GeneID:91417525"
                     /locus_tag="CP976_RS15720"
                     /old_locus_tag="CP976_15760"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /db_xref="GeneID:91417525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="CP976_RS15720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15760"
                     /product="tyrosinase family protein"
                     /protein_id="WP_150480926.1"
                     /transl_table=11
                     /translation="MAYVRKDAGLLTKAERVRFVKALLEVKRRGEYEEFVRMHIEYFSA
                     DGEHGLRAAHMAPSFLPWHRRFLLDLERALRRVDSSVTVPYWDWTRDRTTTSAPWTADL
                     LGGNGRRSDLQVMTGPFAHREGNWTITTGVTDLKFLTRDLGRRRARIGLPTKRELEWAL
                     KDPVYDVAPWNSTVPRGFRNKLEGWGGGRGATSWRNHNRVHGWVGGAILGGASVNDPVF
                     WLHHAFVDLQWHRWRLRHRRARYLPATPPGQGDEQHARIVARHQTLPPWDVTPDELEDV
                     SHIYRYA"
     gene            complement(5001..5504)
                     /db_xref="GeneID:91417526"
                     /locus_tag="CP976_RS15725"
                     /old_locus_tag="CP976_15765"
     CDS             complement(5001..5504)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91417526"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="CP976_RS15725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15765"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_150480927.1"
                     /sec_met_domain="melC (E-value: 1.9e-45, bitscore: 153.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVDIGAVSVGAAGAAPGPPRPRTEQATRRDAVRGLAAGALAAVM
                     VPFATASRPRRPGPAPGNAVLDTHFDQTYSGRRIRGVWIPERGTAEAGRWQVSVDGRPL
                     HLMRRADGTWLSMVDHYCSYRSPLEAARAAVDQLGPGQRLREPARGTGGAGHVHKGSRR
                     GVRT"
     misc_feature    complement(5493..5507)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    5529..5544
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.55"
                     /tool="antismash"
     misc_feature    complement(5539..5556)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.78"
                     /tool="antismash"
     gene            complement(5647..6228)
                     /db_xref="GeneID:91417527"
                     /locus_tag="CP976_RS15730"
                     /old_locus_tag="CP976_15770"
     CDS             complement(5647..6228)
                     /codon_start=1
                     /db_xref="GeneID:91417527"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP976_RS15730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CP976_15770"
                     /product="hypothetical protein"
                     /protein_id="WP_150480928.1"
                     /transl_table=11
                     /translation="MKSLKAAAVFAGSLIAAGSATPAFAYNAPQQAPTGYSAPDQAPAA
                     QSAPYYASTPQAAPQQAPAPYQAPAPQQAAPQQAPAPYQAPAPQQAAPYQAPAPQQAAP
                     HQAPAPQAAPYQAPAPQQAPDPRPAPDQAPAATEGHRTRDLAATSVSGDMTTRVPLDVT
                     AEGSNALLPTAKEAVAALNEAKPAHGNLWL"
     misc_feature    complement(6339..6355)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.39"
                     /tool="antismash"
     gene            complement(6462..7145)
                     /db_xref="GeneID:91417528"
                     /locus_tag="CP976_RS15735"
                     /old_locus_tag="CP976_15775"
     CDS             complement(6462..7145)
                     /codon_start=1
                     /db_xref="GeneID:91417528"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP976_RS15735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CP976_15775"
                     /product="hypothetical protein"
                     /protein_id="WP_150480929.1"
                     /transl_table=11
                     /translation="MSALPARRIASSALCAALLVGITGPVAMAADSARGHAHVAPGARL
                     PGADARLAQIAKLNRGELTPVADLLNAVLDSNGLLPAAEARKLGAGAKAALAEAAAKDP
                     RIPVTSTVVLLPAPAPALPALVPAAPERRAADPVTDLLDLVLGAVDSLLQAITSGVGGI
                     LPSVDGLLNGVDDLLAGLLGGNLLQDEPVSSTPSSTSSTSVSTTPEAVTYPAVTLLPPA
                     QPTAS"
     gene            7546..8634
                     /db_xref="GeneID:91417529"
                     /locus_tag="CP976_RS15740"
                     /old_locus_tag="CP976_15780"
     CDS             7546..8634
                     /codon_start=1
                     /db_xref="GeneID:91417529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="CP976_RS15740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15780"
                     /product="DUF3344 domain-containing protein"
                     /protein_id="WP_150480930.1"
                     /transl_table=11
                     /translation="MRHPLGPVLRRATVGVLALAAMWATSSSPAAAPAAAPAPEAESLG
                     FTGRYLTLQHGGIARAANASITCRTTQGPSCRDAQAGGSGVNGDFDMFYVDVDRDPNTY
                     NSSRAEVRLPNGSHVTYARLYWGGNLRVGEQKPPQDNGRVLIAEPGGEYKELVADTTVG
                     HRVADGMDAFQASSDVTRLVREGGPGLYTVAQVNVAMGSSAAGAWGGWTLVVVYENPSE
                     PLRHLALWDGFTPLNSGAGQEIPLTNLGFAAGASGRAGLVAYNGDRGTTGDALTMTTGQ
                     SEVALGNAANPQDDVLNSTISEPGADAARVPAHANNLGYDSDVFDLGRGLQHAGDQATF
                     RLHSQQDAAWAGVLFVAVDARR"
     gene            8685..9935
                     /db_xref="GeneID:91417530"
                     /locus_tag="CP976_RS15745"
                     /old_locus_tag="CP976_15785"
     CDS             8685..9935
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /db_xref="GeneID:91417530"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990352.1"
                     /locus_tag="CP976_RS15745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_15785"
                     /product="glycosyltransferase"
                     /protein_id="WP_150480931.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.7e-31,
                     bitscore: 108.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLSSTVPLPRVLHLTQPVDGGVARVVTDLVRAQLSDGLDVTVAC
                     PDSALTTKLRTLGARVRHWHATRSPGTSLVREVRHLARVIDEVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVSGGTAALALMWERWGARWASRVVCVSEAERATGMGAR
                     ITGRWAVVPNGIDPERFHPAAAGAVRAGLPPLAGIHPAAPLAVCVGRLCRQKGQDVLLR
                     AWEAVVRQVPDARLVLVGDGPDHDRLREGAPESVVFAGAVADASPWYQAADLVVLPSRW
                     EGMALAPLEAMASERPVVVTDVDGARESLPPSFAARCLVPPEDPAALAGAVTELLADPL
                     LRESLGHQGRRHVLSTHDVRYTAEAVSDVYRELLGPWTATTPSAEHGQPLGTGHGAEVN
                     RTTQRVEPTEYRESIHS"
ORIGIN
        1 gtccaaggtc accacatgtg aagaatgccc aactttgggg gaattctgaa gcatatgagc
       61 atgtctgcgc gccgatggaa cacaagggat ggaagggact actgggacac gggcccactc
      121 caggcataac gagtgcacaa cacccgatgc ctctgacgcc aaaagcggtg cacgattcgt
      181 acctgcctgc gattatcagg accgcttcac cgaacctcca catcgagcca cggaggcgaa
      241 gcaacaggga caaggacgcg ggagccctat ccttgtcccc ggcaacgtca cggatcggcc
      301 cacaccaggg caccacgaca gaggccacca caccggcccc cgtagctcag tggatagagc
      361 aggcgccttc taagcgcttg gccgcaggtt cgagtcctgc cgggggcgca agtctccgcc
      421 ctcccctcgg gagggcgttt ttaccgttcg ggccttgtca tcgactgcgc accactccag
      481 gacgcgccgg ccgacgatga ggcgggcgac tgtcccggcg cggtggcgac cggtcggctg
      541 gacgggggcg gctgctcgcc gagcacgtgg tgtcctcggc cgggcccggt atcgcaccgg
      601 gttacgcgga gcgggccgcc cggctccccg gcgtggcggc ggcctccggg cagctgtccg
      661 tggacggcga actgagccgc gcggacgccg accaggacga cgacgcgtcc gtcatcggcg
      721 gaaacctggt cgtgatgcgg gccgacgcgg tgcccgatct cctcgacctc ggggtccaca
      781 ccggctcgct cgcccgcctc ggcgagacgt ccgtggcgct cgcatggtga ccgcaggccg
      841 ccgagggaag ttcgccctgc tgcggctggc cggcgcgcag tcggcgcagg tgctgtggat
      901 gccggcctgg gaagccgtgc tcacctgcgc gatcgcgttg ctgccggccg ctctggtctg
      961 cgcggcggtg ctggtgccgc tcagcatcgc ggtcaccggt tccgccgtgc cggccttggc
     1021 cggcgggccg cttgccgcgc tggccgccgg ggctcccctg gtggccgtcg cgacgaccac
     1081 cctcttcggc cgctcggcca tgcgcagtgc cgcggccacg cccggaggcc tggcgagggc
     1141 cgtctccctg acacccccgc cgtgtcccga gggtcccacc ccgcttcccg cagcccggct
     1201 caggcccgat catggtccgg ggaacccgtc gtgggccgag gaaggggtgt gacgtgcggc
     1261 tgcgctgtgc tgtgctggac gactttcaga gggtggcgac cgaagtcgcc gactggtcgc
     1321 cggtcgccga cgaggtggag gtcgtctcct tcgacaccca catggacggc gaggacgccc
     1381 tggccgcggc cctggccgac ttcgacatcg tggtgacgtt gcgggagcgc gtgcccttcc
     1441 cggggtcact gatcgcccgg ctgccccggc tgaaactgat cgtcgcctcc ggcctgcgca
     1501 actcggtcat cgactacgcg gccgccgagg agcacggcgt caccgtgtgc ggcaccacga
     1561 gttcctccac cccgccggtg gaactgacat gggccctgct gctcggcctc gcccgcggcc
     1621 tcgtcgagga gagcaacgcc ctgcgctccg gcggcccctg gcagtccacc gtcggcgccg
     1681 acctgtacgg cagccgcctc ggacttctcg gcctcggcaa gatcggcagc cgcgtggcgc
     1741 gggtcggcct cgccttcggc atgcacgtca gcgcgtggag tcagaacctc accaaggagc
     1801 gcaccgacga gatcggcgtc gaactcgccc cctcccggga ggacctcctc gccggcagcg
     1861 acttcgtctc gatccacctc gcactgagcg accgcacccg cggactgctc ggacccgccg
     1921 aactcgccct cctcaagccg accgcgtacc tgatcaacac ctcgcgcgcg gccatcgtcg
     1981 accaggaagc cctcctcgcc gccctgcacg agggccggat cgccggcgcc ggcgtcgacg
     2041 tcttcgacat cgagcccctg cccgccgacc acccgatgcg caccgccccc cgccttctcg
     2101 cgaccccgca cctcggttac gtctcccagg ccaactacgc gacgtactac ggacaggcgg
     2161 tcgagaacat tcgcgcgttc ctctcgggcg cgccggtgcg acggctgccc tgacacactc
     2221 gccgggcgcc gcatgcgagc agtagcggcc cccggcacag caaatggccg ggaccctgta
     2281 ccgggtcccg gccatccctg accgtcgggt ggacttacgc gacaggcgcc gacgtgaaca
     2341 aaggcgcgtc ggctgcctcg gaagtcagga ctccttgccg ccgatgacga cactgctgtt
     2401 gcagccctcg ctggaacagt tctgcgcttg gctcgcgacg acgtggacgt ttccgtctcc
     2461 gtcgcggtat acggcctcgt gcacgcagcg aacattcccc ttgccgtcat cgacgcactt
     2521 gccggagcca ctggacccgc cggagccgtc aagggcgaag gcctggccgg ccccggctcc
     2581 aaggagagcg acgctccaca gaactccagc tacgacggcg atcctctttg cattcggcat
     2641 gtccatactc ctttttgcat cgattccccg ggtggacgac ggggaccttc gagaacttgc
     2701 atcggcaaaa gccaggggcg ggccccggac cccgaaaatg ttcgagtctc ggagcccgcc
     2761 ctgatcagct gttcagtgac cgccgccgag agcgaaggcg ttggtgcagg tcaccgactt
     2821 gctctggtca acgggctggt tcgccagaat gtccaggatc tggacgttga tgcggccgat
     2881 gttcagcacg cccgactggc tctcgttgcc gcaggtgttg gactgcttgc tgtccacctc
     2941 gagcgccggc ccgtcgccgg cctggctgac gccggcgccg aggaaggtga cggtgccgag
     3001 cgcggccatg acgaccgcag ccttctggaa cttgcgcatt gcttctccgt ttctcacgtc
     3061 gcacacggtc cgttatgaac cgcctaccga cggtgttcaa gctaggagag attcgcggct
     3121 tttgacaggc gaaacgccga gtcgcgtaat tttcttgcca cgccgtgcgg ggatccgcgc
     3181 caacgccacg agcgcggctc cgcacagcga cgagcgggcc ccgtgtccgg acacatggtc
     3241 cgaacgccgg ggcccgctca ggtcacctgg tcagcgatcg tcagccaccg atgccgaagg
     3301 cgttggagca gggcaccgag gtgctctggt cgaccggctg gttcgccaga atgttcagga
     3361 cctggacgtt ggcgcggccg atgttcagca cgcccgactg gctctcgttg ccacaggtgt
     3421 tggactgctt gctgtccacc tcgaacgccg gcttgtcgcc ggcctggctg acgccagcac
     3481 cgaggaagct gacgctgccg agcatgaccg cgacgactgc ggccttctgg tacttgcgca
     3541 ttacttctcc gtttcttgag tcgacacgat ccgtgacgga ccggtcactt acagtttctg
     3601 acagtagacg attttttccc acctttcaca ggcaaaacgc acacatgcgc aactttcatg
     3661 aaaaaaccat cgttggggca gccgtgaccg gtcccctgcc gccgcgttga aggggcagtg
     3721 cggtcgcgcg tcccgctgcc gcactcatcc gagtcactaa ggagaagtga tgtctcgcat
     3781 cgcgaaggtt ggcgctgtcg cactgggcac gggcgccatg gtgctcagcg gggccggtct
     3841 ggccgcggcg gacgccgacg ccaaggctgc ggcgatcggc tcgcccggct tcctgtccgg
     3901 caacgtggtc caggtcccga tccacgtgcc gatcaacgtg tgcggaaaca ccgtcaccgt
     3961 gatcggtgca ctcaacgagt ctgccggcgt ctgcaagaac ggcaacgacg aaaaggacaa
     4021 gaagggtaag aaggacaaca agaaggccga caagaaggcc cttcaccacc acggccgctg
     4081 agtctgccgt tttccccaag agccccggcg tgtctcagcg tcggggctct tgccgtgaag
     4141 cctcggcatc tcacgcgtac cggtagatgt ggctgacatc ctccagttcg tccggtgtca
     4201 cgtcccacgg cggcagcgtc tggtgtctcg ccacgatccg ggcgtgctgc tcatcacctt
     4261 gtcccggcgg tgtggcgggc aggtaacgtg ccctgcggtg acgcaggcgc cagcggtgcc
     4321 actgaaggtc cacgaaggcg tgatgcagcc agaagacggg gtcattgacg gaggctccgc
     4381 cgagtatcgc cccaccgacc caaccgtgca cccggttgtg gttgcgccag gaggtggcgc
     4441 ctcggccgcc accccagcct tccagtttgt tgcggaaccc tcgtggaacc gtcgagttcc
     4501 agggcgccac gtcatacacc ggatccttca gcgcccactc cagctctctc ttcgtgggca
     4561 ggccgatccg ggcgcggcgg cggccgaggt cacgggtgag gaacttcagg tcggtcacgc
     4621 ctgtcgtgat ggtccagttg ccctctcggt gggcgaatgg accggtcatc acctggaggt
     4681 cggatcgacg cccgttgccg ccgaggaggt ccgccgtcca gggcgcggaa gtcgtcgtgc
     4741 ggtcgcgcgt ccagtcccag tacggcaccg tcaccgagga gtccacccgg cgcagcgcgc
     4801 gctccaggtc gagcaggaac cggcggtgcc agggcagaaa cgagggcgcc atgtgcgcgg
     4861 cacgcagccc gtgctcaccg tccgcggaga agtactcgat gtgcatccgc acgaactcct
     4921 cgtactcgcc ccgccgtttg acctccagca gcgccttgac gaagcggact cgctcggcct
     4981 tggtgagcaa gccggcgtcc ttacgtacgt acgccacgcc ggctcccctt gtgcacatgc
     5041 cccgcgcctc cggtgcccct ggccggttca cgcagtcgtt gaccggggcc gagctggtcg
     5101 acggccgcgc gggcggcctc gagcggtgac cggtaggagc agtagtggtc gaccatgctc
     5161 agccaggtac cgtcggcccg gcgcatcaga tgcagcggtc ggccgtccac cgagacctgc
     5221 caccggccgg cctcagccgt gccacgctcg gggatccaca cacctcgaat gcggcggccg
     5281 ctgtaggtct ggtcgaagtg ggtgtcgagc accgcgttcc cgggcgccgg gccggggcgc
     5341 cgaggtcggg aggcggtggc gaacgggacc atgaccgcag cgagtgcgcc cgcggccaga
     5401 cctcgcaccg catctcgccg tgtggcttgt tccgttctgg gccgcggcgg gcccggtgcc
     5461 gcacctgcgg cgccaaccga taccgcgccg atgtcgacga ccatgattcg ctccacgccc
     5521 cttcagttgt ctacagccgt aaccgaggaa caacaagggc ggttaccacc gcgcgaccat
     5581 tgcggtgaca acggaggcgg cccccggaac cactggggac cgcctcctga ctgatgagtc
     5641 tcggccctac agccacagat tgccgtgcgc cggcttggcc tcgttcagcg cagccacagc
     5701 ctccttagcc gtagggagca gcgcgttgga accctcggca gtcacgtcga gcggaacccg
     5761 cgtggtcatg tcgccgctga cgctggtggc tgccaggtcc cgggtacggt ggccctccgt
     5821 cgccgcgggc gcctggtccg gagcaggacg ggggtccggg gcctgctgcg gagcgggggc
     5881 ctgatacgga gcggcctgcg gagccggcgc ctgatgcgga gcggcctgct gcggagcagg
     5941 agcctgatac ggagcggcct gctgaggggc gggggcctga tacggagccg gggcctgctg
     6001 cggagcagcc tgctgagggg cgggggcctg atacggagcc ggggcctgct gcggagcagc
     6061 ctgcggggtc gacgcgtagt acggagcact ctgagcagcc ggcgcctggt cgggggcgct
     6121 gtaaccggtc ggcgcctgct gaggagcgtt gtaggcgaac gcgggggtgg cggaaccggc
     6181 ggcgatcaag gacccggcaa agacggcggc agccttcaga gacttcatcg tgttcctttc
     6241 ttcggcaacg cgcgtcgccg gaggggattc tgtcgccgtg cctaacgagc cctggcctgg
     6301 gcggaaaccg gagaagccca agattgccga tacctcatac gagatctatt aacagaccgg
     6361 gccgcggaac ggaaaagaac cggaggaata cgcgaagtgg ggcgggaaga acaacagtcg
     6421 tggcgccgct ctctgaggtc ggcaccacga ctgggcgtgg ctcaggaggc cgtcggctgc
     6481 gccggcggca gcagcgtgac tgcggggtac gtgacggcct cgggcgtcgt cgacaccgat
     6541 gtcgacgacg tgctggacgg cgtcgaggaa acgggctcgt cctgaagcag gttgcctccg
     6601 agcagtccgg cgaggaggtc gtccaccccg ttcagcaggc cgtcgacgga gggaaggatg
     6661 ccgccgacac cagaggtgat cgcctgcagg agactgtcga cagcccccag cacgaggtcc
     6721 agcaggtcgg tcacggggtc ggcggcgcgc cgctcgggcg ctgcgggcac cagggcgggc
     6781 agggcgggag cgggagccgg caacaggaca accgtcgacg tcaccgggat tcgcgggtcc
     6841 ttggcggccg cctcggccag ggcggccttc gctccggctc ccagcttcct ggcctcggcc
     6901 gcgggcagta ggccgttact gtccagcacg gcgttcagca ggtcggcgac cggggtgagc
     6961 tcaccccggt tgagtttcgc gatctgtgcg agacgcgcat ccgcgccggg gagccgggcg
     7021 ccgggcgcca catgagcgtg cccgcgagcc gagtcggccg ccatggcgac ggggccggtg
     7081 ataccgacca gaagggcggc gcacagggcg ctggacgcga tgcgccgtgc gggcagagca
     7141 ctcatgggga ttcctttcga cggccttgga tcttgagccc accgtgaaag cgcccgcgac
     7201 ttcccgcaac cgctcatacg cctttcagga tttaacaatt cccccattcg ccgtgcccac
     7261 cctgatcaga cggtatgtca ggacgctccc ttctccacct ctgccggagt aaggcaccgg
     7321 ccgcccccgg gtggaggcgc tgaccgacgc aggaggcgac ggccggaacc cgcgaagact
     7381 ggggtttcac gattccgaca cacttgtcag acttcaccga atttcttcat ctatgtccga
     7441 ttggtgacta gagttgcgaa ccgcccgacg cactctcagc gcggacacac cgcactcgtg
     7501 gcgctgctgc cacggccacc tgttccgaaa gggcaacgac cggtcatgcg ccatcctctg
     7561 ggccccgtgt tgcgccgtgc gaccgtgggc gtcctggccc ttgccgccat gtgggcaacc
     7621 agcagttccc cggccgccgc gccggccgcc gcgcccgcac cggaggccga gagcctcggg
     7681 ttcaccgggc ggtacctcac gctccagcac ggcggcatcg cacgcgcggc caacgcatcg
     7741 atcacctgcc gcacgaccca ggggccttcg tgccgggacg cccaggcagg cgggtccggt
     7801 gtgaacggcg acttcgacat gttctacgtc gatgtcgacc gcgaccccaa cacctacaac
     7861 tcctcccgcg cggaggtccg cctccccaac ggctcccacg tgacctacgc gcgcctctac
     7921 tggggcggca acctgcgcgt gggcgaacag aagccgccgc aggacaacgg gcgcgtgctg
     7981 atcgccgaac ccggcgggga gtacaaggag ttggtcgccg acacgacggt cggacaccgc
     8041 gtggcggacg gcatggacgc cttccaggcc tcgtccgacg tgacacggct ggtgcgggag
     8101 ggcggaccgg gcctgtacac cgtggcacag gtcaacgtcg ccatgggcag ttcggcggcc
     8161 ggggcgtggg gtggctggac gctcgtggtg gtctacgaga atccgtcgga gccactgcgg
     8221 cacctcgcgc tctgggacgg cttcaccccg ctcaattccg gtgcaggaca ggaaattccc
     8281 ctgaccaacc tcgggtttgc cgcgggcgcg tcgggccgtg cggggctggt ggcgtacaac
     8341 ggcgaccgcg gcaccaccgg cgacgcgctc accatgacga ccggccagtc cgaggtcgcc
     8401 ctcggcaacg ccgccaaccc ccaggacgac gtcctcaatt ccaccatcag tgagcccggg
     8461 gcggatgcgg cacgtgtgcc cgcgcacgcc aacaacctcg gctacgactc cgacgtgttc
     8521 gatctcggca gaggcctgca gcacgccggt gaccaggcga ccttccggct ccattcccag
     8581 caggacgcgg cgtgggccgg cgtgctcttc gtcgccgtcg acgcacggcg gtgactttgt
     8641 cgccgcccgc cccgaacacc ttcgcgagcg aggaatccgc ccacatgcac ctgtcatcca
     8701 ccgtccctct cccaagagtc ctgcacctca cccaaccggt ggacggcggc gtcgcgcgcg
     8761 tcgtgacgga cttggtccgg gcacaactgt cggacggcct ggacgtcacc gtggcctgcc
     8821 ccgacagcgc gctcaccacg aaactgcgga ccctcggcgc gcgcgtacgg cactggcacg
     8881 cgacgcggtc gccgggtacg tcgctcgtcc gggaggtacg gcacctggcg cgggtgatcg
     8941 acgaggtgcg gcccgatctg gtgcacgcgc acagcgcgaa ggccgggctc gccggccggc
     9001 tcgcggtacg cgggcggatt ccgacggtct tccagccgca cgcctggtcg ttcgaagcgg
     9061 tcagcggggg caccgcggcc ctggcgctca tgtgggaacg ctggggggcg cgttgggcct
     9121 cacgcgtggt gtgtgtgagc gaggcggaac gcgccaccgg catgggcgcc aggatcaccg
     9181 gccggtgggc cgtcgtcccc aacggcatcg accccgagcg cttccacccg gccgccgcgg
     9241 gcgccgtgcg ggccgggctc ccaccgctcg ccggaatcca cccggcggca ccgctcgccg
     9301 tgtgcgtggg gcggctgtgc cggcagaaag ggcaggacgt cctgctgcgg gcctgggaag
     9361 ccgtcgtgcg gcaggtgccc gacgcccgcc tcgtcctggt cggcgacggg ccggaccacg
     9421 accggcttcg tgagggcgcc ccggaatcgg tggtgttcgc gggagccgtc gccgacgcct
     9481 ccccctggta ccaggccgcc gatctcgtcg tcctgccgtc ccgctgggag ggcatggccc
     9541 tggcgccact ggaggcgatg gccagcgagc ggcccgtagt ggtgacggac gtcgacggcg
     9601 cccgcgagag cctgccgccc tccttcgccg cccggtgcct ggtcccgccg gaggacccgg
     9661 cggcgctggc cggggccgtc accgagctgc tggccgaccc actgctgcgc gagtcgctcg
     9721 gccatcaggg ccgccggcac gtgctgtcca cccacgacgt gcggtacacc gccgaggccg
     9781 tctcggacgt ctatcgcgag ctgctcggcc catggaccgc caccaccccc agcgctgagc
     9841 acggtcagcc gctcggcacc ggacatggcg ccgaggtgaa ccggacaacc cagcgcgtcg
     9901 agcccaccga gtacagggag tccatccact cgtgaccgcc gaacgtaccg tgccgtcccc
     9961 cggcgtaccg ccacgggacc acggatcctc accagtctcg gtcatgccgc cgcgcggcac
    10021 cgccaccggt ttccggtcgt ccgccccgcg gggcccggcc cggcccgcat cccccgtccc
    10081 gctgctcgtc gcggacggcg tcgccgccct ggcgggcgcg ccggcgctca ccgatgtcca
    10141 gcgccagcca ctgctcgtca ccctgctgat cgtcatgtcg ctgctgctgc gccggcgcga
    10201 cgggcggccg ccggtaccgg ggttgctgga ggaactgccc gccgtgtgcg gccgcatcgc
    10261 ggtggcctgg ctggcactcg cggcgctcct ggcggcgtac cggccggagc acgcgttgtc
    10321 cctccgtacc gtgctgacgg gcctcgccct gcacgcggcg gccggctgtg tgctgcgcgg
    10381 agccgtgcac gcgcggcggc gcgccgcgct gctgcgccac ccgcacaccg cactcgtcct
    10441 cggaccggcg gcgaccgcgc agcgcgtggc cgccgccgta ctgcgtcacc cgcggtgcgg
    10501 cgtg
//
