LOCUS       NZ_CP023694            10276 bp    DNA     linear   CON 13-JUL-2024
DEFINITION  Streptomyces coeruleorubidus strain ATCC 13740 chromosome, complete
            genome.
ACCESSION   NZ_CP023694
VERSION     NZ_CP023694.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces coeruleorubidus
  ORGANISM  Streptomyces coeruleorubidus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9335698)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008705135.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:16:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,499
            CDSs (total)                      :: 8,405
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 96 of 229
            Pseudo Genes (incomplete)         :: 175 of 229
            Pseudo Genes (internal stop)      :: 12 of 229
            Pseudo Genes (multiple problems)  :: 53 of 229
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:54:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2764753
            Orig. end                         :: 2775029
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5276
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(3..3011)
                     /db_xref="GeneID:91416972"
                     /locus_tag="CP976_RS12835"
                     /old_locus_tag="CP976_12865"
     CDS             complement(3..3011)
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /GO_function="GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91416972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037023.1"
                     /locus_tag="CP976_RS12835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12865"
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="WP_150480476.1"
                     /transl_table=11
                     /translation="MTAAPGRRSSTFTRLLRHGFTDASAAERLLDSAELAPLRDDPVLL
                     DALGATADPDLALLGLVRLLEAQPEPGARRELLDTLISSKPLRDRLLGVLGASTALADH
                     LTRHPDDWQALVMYEAHDLHPGVEEFEDGLAQATDPVSLRVAYRRCLLSIAARDVCGTT
                     GVAESAAELADLATATLRAALGLAGAAAPEDAALCRLAVIAMGKCGGHELNYVSDVDVI
                     FVGEAVEGADETKAVRAATRLASHMMRICSETTVEGSIWPVDANLRPEGRNGPLVRTLS
                     SHLAYYQRWAKTWEFQALLKARPVAGDLDLGAEYVAAVQPLVWKAAERENFVPDVQKMR
                     RRVVENIPVAEVDRQLKLGPGGLRDVEFAVQLLQLVHGRTDTSLRSGTTLDALKALAAG
                     GYVGRADAAQLDEAYRFLRSMEHRIQLFRLRRTHLVPEDEAELRRIGRSLGLRTDPVAE
                     LLREWKRHATVVRRLHEKLFYRPLLDAVAQLATGEARLSAEAARERMVALGYADPASAL
                     RHLEALASGVTRKAAIQRTLLPVMLGWFADSADPDAGLLNFRKVSDALGKTPWYLRLLR
                     DEGAAAQNLARVLSAGRLAPDLLMRAPEAVALLGDGDGGGLEPRSRAHLEQEILAAVNR
                     ADGAAQAVTAARGVRRRELFRTAAADIVGSYGTEEQPVEADPGALVDRVGGAVSDLTAA
                     TLAGTLRAVVRDGWGDRLPTRFAIIGMGRFGGHELGYGSDADVLFVHEPRDGVDEREAG
                     QAANKVVSEMRRLLQIPSADPPLLIDADLRPEGKSGPLVRTLTSYAAYYRRWSLTWESH
                     ALLRAEHVAGDEDLGRRFIELIDPLRYPADGLADEAVRDIRRLKARMESERLPRGADPK
                     LHAKLGPGGLSDVEWTVQLLQLRHGAAEPGLRTTRTRQALAAARTAGLIGDEDAAVLDE
                     AWVLATRVRNAVMLVRGRAGDTFPTEPRELAAVGRYLGYGPGHAGDMLDAYRRTARRAR
                     AVVDELFYGVTER"
     misc_feature    3133..3148
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.67"
                     /tool="antismash"
     gene            3170..3688
                     /db_xref="GeneID:91416973"
                     /locus_tag="CP976_RS12840"
                     /old_locus_tag="CP976_12870"
     CDS             3170..3688
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /GO_function="GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91416973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115923.1"
                     /locus_tag="CP976_RS12840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12870"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_150480477.1"
                     /transl_table=11
                     /translation="MTTMHPETRLDRRYSDETATATDWQEAEARLTAAELFWITTVRPD
                     GRPHVTPLPAVWSDGALHFCTGPEERKARNLAENPHVVLTTGTNTWNEGYDLVVEGEAT
                     RVTDDGRLRELAAAWEAKYGSFWHFEVREGYFRHGAGHAAVYAVAPRTVFGFGKGEPFS
                     QTRWRFTDA"
     gene            3704..4150
                     /db_xref="GeneID:91416974"
                     /locus_tag="CP976_RS12845"
                     /old_locus_tag="CP976_12875"
     CDS             3704..4150
                     /codon_start=1
                     /db_xref="GeneID:91416974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326162.1"
                     /locus_tag="CP976_RS12845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12875"
                     /product="VOC family protein"
                     /protein_id="WP_059420767.1"
                     /transl_table=11
                     /translation="MDFTLEVIPLPVTDIDRARDFYRDKVGFHVDIDQEVMPGMRIVQL
                     TPPGSGCSIALGDSIWEMTPGPTPAPGSYQGLQLCVADIKAAHAELVERGLDVSEPVQY
                     APDDGATFMHFQDPDGNGWAIQEYRRRATEPLHQLLADLKRQGG"
     misc_feature    4684..4698
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.73"
                     /tool="antismash"
     gene            4825..5097
                     /db_xref="GeneID:91422855"
                     /locus_tag="CP976_RS43650"
     CDS             4825..5097
                     /codon_start=1
                     /db_xref="GeneID:91422855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP976_RS43650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_229875273.1"
                     /transl_table=11
                     /translation="MALAAFSFATPAAADASQAATVTANGTVVAADGSKVGQVTVHKRG
                     DGTKPPAELGNPSEWGVVVIKMDDSTFTPQACMVPVAEIGATATT"
     gene            5001..5276
                     /db_xref="GeneID:91422856"
                     /locus_tag="CP976_RS43655"
     CDS             5001..5276
                     /codon_start=1
                     /db_xref="GeneID:91422856"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="CP976_RS43655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_229875254.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 8.4e-12,
                     bitscore: 44.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGCGGDQDGRFDLYAAGLHGASGGNWCYGYNLTTDGKYCYSNYYH
                     STKDHHSTVKIAGGSNTGYAFAGQTSYANRTAGAAYTCETFYSLDE"
     misc_feature    5501..5517
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.75"
                     /tool="antismash"
     misc_feature    complement(5638..5653)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 24.96"
                     /tool="antismash"
     gene            6193..7017
                     /db_xref="GeneID:91416975"
                     /locus_tag="CP976_RS12865"
                     /old_locus_tag="CP976_12895"
     CDS             6193..7017
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91416975"
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /locus_tag="CP976_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12895"
                     /product="site-specific integrase"
                     /protein_id="WP_308429989.1"
                     /transl_table=11
                     /translation="MIYNNVSTVLNAAVDDGHLSKNPCHAQSVCPPAAGSGRVVPWSAE
                     RVFAVRAALPERYRATVDLGGGCGLRQGEIFGLPVDAIGFDSGWLHIANQVKVTKDGLV
                     FAPPKRNKIRDVPLPNRVAHVLKQHMEAFPPVEITLPWLRVGGQSVTKRLLFTRLDGAG
                     AVRRTDFNDRAWKPALVAAGVIPAPNPGERHQAAREHGMHALRHFYASALLDTGENVKA
                     LSNYLGHNDPGFTLRVYTHLMPSSDARARKAVDGLYEGTDPAPDGPETAQGL"
     gene            complement(7093..8454)
                     /db_xref="GeneID:91416976"
                     /gene="glnA"
                     /locus_tag="CP976_RS12870"
                     /old_locus_tag="CP976_12900"
     CDS             complement(7093..8454)
                     /EC_number="6.3.1.2"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91416976"
                     /gene="glnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931998.1"
                     /locus_tag="CP976_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12900"
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="WP_150480481.1"
                     /transl_table=11
                     /translation="MDKQQEFVLRTLEERDIRFVRLWFTDVLGFLKSVAVAPAELEQAF
                     DEGIGFDGSAIEGFARVYESDMIAKPDPSTFQILPWRAEAPGTARMFCDILMPDGSPSF
                     ADPRYVLKRALARTSDLGFTFYTHPEIEFFLLKDRPLDGSRPTPADNSGYFDHTPTNVG
                     MDFRRQAITMLESMGISVEFSHHEGAPGQQEIDLRYADALSTADNIMTFRLVMKQVALE
                     QGVQATFMPKPFSEHPGSGMHTHLSLFEGDRNAFYESGSEYQLSKVGRSFIAGLLRHAA
                     EISAVTNQWVNSYKRIWGGAERTAGAGGEAPSYICWGHNNRSALVRVPMYKPGKTGSAR
                     VEVRSLDSGANPYLSYAVLLAAGLKGIEEGYELPPGAEDDVWALSDAERRAMGIEPLPQ
                     NLGEALTLMERSDLVAETLGEHVFDFFLRNKKSEWEEYRSEVTAFELRKNLPAL"
     misc_feature    complement(8690..8706)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.06"
                     /tool="antismash"
     misc_feature    8691..8705
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     gene            8749..9939
                     /db_xref="GeneID:91416977"
                     /locus_tag="CP976_RS12875"
                     /old_locus_tag="CP976_12905"
     CDS             8749..9939
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91416977"
                     /gene_functions="other (smcogs) SMCOG1094: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037010.1"
                     /locus_tag="CP976_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP976_12905"
                     /product="globin domain-containing protein"
                     /protein_id="WP_150480482.1"
                     /transl_table=11
                     /translation="MLSEQSAVTVRATLPAVGAALGTITERFYAGLFAAHPELLNDLFN
                     RGNQAAGTQRQALAGSIAAFATHLLDHPEQRPDAMLHRIAHKHASLNITPEQYDIVHEH
                     LFAAIADVLGDAVTPEVAAAWDEVYWLMANALIAVEKRLREERGGYAWRAWEVVERVAE
                     TDDVATFRLRPADGGPVRDFLAGQYVSVRVALADGARQIRQYSLSGAPAPDLRQISVKR
                     VHGDGTPDGEVSNHLHARVQAGDVLELSEPYGDLVLDAGPDTPLLLASAGIGVTPMTAM
                     LAHLAGSGHRAPVTVVHGDRSPAAHALRTDHEAYAAKLPDASVHFWYEQDAPAGTRSGL
                     VDLADVPVAPGTRAYLCGPLPFMRAVRAQLIGKGVAPADIHYEVFGPDLWLAGQAA"
ORIGIN
        1 gactagcgct ccgtcacccc gtagaacagc tcgtccacca cagcccgggc ccggcgtgcc
       61 gtacgccggt acgcgtcgag catgtccccg gcgtggccgg gtccgtagcc caggtaccgt
      121 cccacggcgg ccagttcacg gggctccgtg gggaacgtgt cgccggcccg gccgcggacc
      181 agcatgaccg cgttgcggac gcgggtcgcg aggacccagg cctcgtccag gaccgccgcg
      241 tcctcgtccc cgatgagacc ggccgtgcgg gcggcggcca gggcctgacg cgtccgggtc
      301 gtgcgcaggc ccggctcggc cgcgccgtgc cgcaattgca gcagctgcac cgtccactcc
      361 acgtcggaca gaccgcccgg gcccagcttg gcgtgcagct tggggtcggc accccgcggc
      421 agccgctcgg actccatgcg ggccttcagc cgccggatgt cgcggacggc ctcgtcggcg
      481 agcccgtccg ccgggtagcg cagcgggtcg atcagctcga tgaaccggcg gccgaggtcc
      541 tcgtccccgg cgacgtgttc ggcccgcagc agcgcgtgcg actcccaggt cagggaccac
      601 cggcggtagt acgccgcgta cgaggtgagg gtccgcacca acgggccgga cttgccctcc
      661 gggcgcaggt cggcgtcgat gagcaggggc gggtcggcgc tggggatctg gagcagccgg
      721 cgcatctcgg agacgacctt gttggcggcc tgcccggcct cccgctcgtc gacgccgtcg
      781 cgcggctcgt gcacgaacag cacgtccgcg tcggaaccgt agcccagttc gtgtccgccg
      841 aagcgtccca tgcctatgat cgcgaaccgg gtgggcagtc tgtcccccca gccgtcgcgc
      901 acgaccgcgc ggagcgtacc cgcgagggtc gcggccgtca ggtccgacac cgcgccgccc
      961 acccggtcca ccagggcgcc cgggtcggcc tcgacgggct gctcctcggt gccgtaggag
     1021 ccgacgatgt cggcggcggc cgtacggaac agctcgcggc ggcgcacacc gcgggcggcc
     1081 gtgaccgcct gggcggcccc gtcggcccgg ttcaccgcgg cgagtatctc ctgctccaga
     1141 tgggcccggg agcgcggttc gaggccgccg ccgtccccgt cgccgagcag cgccaccgcc
     1201 tccggggcgc gcatcagcag gtcgggcgcg agccggccgg cggacagcac gcgggccagg
     1261 ttctgcgcgg cggcgccctc gtcccgcagc agccgcaggt accagggcgt cttgccgagc
     1321 gcgtccgaca ccttgcggaa gttgagcaga cccgcgtccg ggtcggccga gtcggcgaac
     1381 cagcccagca tgacgggcag cagggtgcgc tggatggcgg ccttgcgggt gacgccggac
     1441 gccagtgcct ccaggtggcg cagggcggag gccgggtcgg cgtatccgag ggcgaccatc
     1501 cgctcccggg ccgcctcggc actcagccgc gcctcaccgg tggcgagttg ggcgaccgcg
     1561 tcgagcagcg gccggtagaa cagcttctcg tgcagacgcc gtacgacggt ggcgtgccgc
     1621 ttccactccc ggagcagctc ggcgaccggg tcggtgcgca ggccgaggga gcggccgatg
     1681 cggcgcagct ccgcctcgtc ctcgggcacg aggtgggtgc gccgcagccg gaacagctgg
     1741 atccggtgct ccatggagcg caggaagcgg taggcctcgt cgagctgggc ggcgtcggcc
     1801 cggccgacgt acccgccggc ggcgagcgcc ttcagcgcgt cgagggtggt gccgctgcgc
     1861 agcgaggtgt cggtccggcc gtgcaccaac tggaggagct gcacggcgaa ctcgacgtcc
     1921 ctgaggccgc ccgggcccag cttcagctgg cggtcgacct cggcgacggg gatgttctcc
     1981 acgacccggc ggcgcatctt ctgcacgtcc gggacgaagt tctcgcgctc ggcggccttc
     2041 cacaccagcg gctggacggc ggcgacgtac tccgcgccga ggtcgaggtc accggccacc
     2101 gggcgggcct tgagcagcgc ctggaactcc caggtcttgg cccagcgctg gtagtaggcg
     2161 aggtggctgg agagcgtccg cacgagcggg ccgttcctgc cctcgggccg gaggttggcg
     2221 tccacgggcc agatggaccc ctcgacggtc gtctccgagc agatccgcat catgtgcgag
     2281 gcgagccggg tcgcggcccg gacggccttg gtctcgtcgg cgccctcgac ggcctccccg
     2341 acgaagatga cgtccacgtc cgagacgtag ttcagctcgt ggccgccgca cttgcccatc
     2401 gcgatgaccg cgagccggca cagggcggcg tcctcgggcg cggcggcccc ggcgaggccg
     2461 agagcggcgc gcagggtcgc cgtggccagg tcggcgagct cggcggcgga ctcggcgacg
     2521 ccggtggtgc cgcacacgtc ccgggcggcg atggacagca ggcagcgccg gtaggcgacg
     2581 cgcagggaga cggggtcggt ggcctgtgcg agaccgtcct cgaactcctc cacgccgggg
     2641 tgcaggtcgt gggcctcgta catgacgagc gcctgccagt cgtccgggtg ccgggtgagg
     2701 tggtcggcga gggcggtgga ggcgccgagc acaccgagca gccggtcacg cagcggcttg
     2761 gacgagatca gcgtgtcgag cagctcccgg cgggcgccgg gctccggctg ggcctccagc
     2821 agccggacca gcccgagcag cgcgagatcg gggtcggcgg tggcgcccag cgcgtccagc
     2881 agcaccgggt cgtcgcgcag gggcgcgagc tcggcgctgt ccaggagccg ctcggcggcg
     2941 gaggcgtcgg tgaagccgtg ccgcagcagc cgcgtgaagg tactgctcct gcgccccggc
     3001 gccgccgtca tccgggcctc cctcgcactt gcatcggagt cacatcgaaa gtctcatcgg
     3061 atcaaggtcg tacggcttga gcctaaccgc acgggccggg ggaagcgccg atgagttcgg
     3121 cgcccggacg gggtctgcac ctgtgaaccc acaggccggg aggacgctca tgaccaccat
     3181 gcaccccgag acccgtctgg atcgccgcta cagcgacgag acggcgaccg cgaccgactg
     3241 gcaggaggcc gaggcgcggc tcacggccgc cgaactgttc tggatcacca cggtgcgacc
     3301 cgacggccgc ccgcacgtca ccccgctgcc ggcggtctgg tcggacggcg ccctgcactt
     3361 ctgcacggga ccggaagagc gcaaggccag gaacctcgcc gagaacccgc acgtcgtgct
     3421 gaccaccggc acgaacacct ggaacgaggg ctacgacctg gtcgtcgagg gcgaggcgac
     3481 gcgcgtgacc gacgacgggc gcctacgcga actggccgcc gcgtgggagg cgaagtacgg
     3541 cagtttctgg cacttcgagg tacgggaggg ctatttccgc cacggtgcgg gacacgcggc
     3601 cgtctatgcg gtggcgccgc gcacagtatt cggcttcggt aagggggagc cgttcagcca
     3661 gacgaggtgg cggttcaccg atgcctgacg aaggagtcac gccatggact tcacactcga
     3721 agtcatcccg ttgccggtga ccgacatcga ccgtgcccgg gacttctacc gggacaaggt
     3781 cggcttccac gtggacatcg accaggaggt catgcccggg atgcggatcg tccagctgac
     3841 gcctcccggc tccggctgtt cgatcgccct cggcgactcc atctgggaga tgaccccggg
     3901 ccccaccccg gcacccggct cctatcaggg cctgcaactg tgcgtcgccg acatcaaggc
     3961 cgcccacgcg gagctcgtcg aacgcggtct cgacgtctcg gagccggtcc agtacgcccc
     4021 cgacgacggc gccacgttca tgcacttcca ggacccggac ggcaacggct gggcgatcca
     4081 ggagtaccgc cgccgggcga cggagccgct gcaccagttg ctggcggacc tgaagcggca
     4141 ggggggctga ccgggtcggt tcagcgcgta cctggcgaag ttgtaagaac ggacgagggg
     4201 gccggcgatc atcgaccgct ggcccctccc ccttgccgtc caggagacgg caccgccctt
     4261 acagcgccgg gaggttcttc cgcagcaact tcttgttccg aaggaagtga cgtcggcgtg
     4321 gggcgacctc acacgatcgg tgtcggcgca ggtctgacct gtgatcgggt ggtcgttgtt
     4381 ggtcgttgtt ggtcgccttg ggccgccctt cgacggccca cagacggccc ggcccggacc
     4441 gcgtctacgg ctggcggctg cgagcgggag acctcgaccc cctcacggcc ggagaaatct
     4501 tcgacgccta ctgcaccgac atcgactccg gcgaccccat ctcaccggaa tacgacgtgg
     4561 ggcaaatccg gtacgggtcg cgaggctttg acccgtaccg taggcccaca aaagtccagg
     4621 tcaaggacgg cgaacaggcc acaccctgcc gaatgctgca ctacagtcct gtaatggaca
     4681 gtcctttttc ggacagtcct catcgaggct attgactgcc acccatcccc tcgcccaaga
     4741 tcattacgca aactcccact ggggggttag ggaagaaatg ccgtgaggat atccggtaga
     4801 tcgatcgttc tcggctttgt gagcgtggca ctcgcagcat ttagcttcgc tacgccggcc
     4861 gccgcagacg cctctcaggc tgccaccgtt acggcgaacg gcactgtcgt cgccgcggat
     4921 ggttccaagg tcggccaggt caccgtccac aagcgtggtg acgggacgaa gccgcccgca
     4981 gagctgggta acccctcaga atggggtgtg gtggtgatca agatggacga ttcgaccttt
     5041 acgccgcagg cctgcatggt gccagtggcg gaaattggtg ctacggctac aacctgacca
     5101 ccgacggtaa gtactgctac tcgaactact accactccac taaggaccac cattccacgg
     5161 tcaagattgc cgggggttcg aataccggtt acgcgttcgc tggccaaacc tcctacgcca
     5221 accggacggc gggcgctgcc tacacctgcg aaaccttcta cagccttgat gagtagattc
     5281 tgatccccgc atcacgcggt gaccctttcg gtaggtgggg taggttggtg cgccactcgg
     5341 gtctgtttcg tcgcccgcgc agtctccgag tggcgccacc tcctactctg aggtaatcac
     5401 ggaacaggga agaagaaatc gcaaactttc cgcgcactca tgttgcgccg ctcgcagtgc
     5461 ccctcggggt cggttttcta cttggactag cgggtcccct taccgaaaaa tggggcaatc
     5521 ccgtttgcgc ccctctaaat gttgtttttt ccgatggatg gtcgtgggtc tgttatgcat
     5581 tcttggtagg ctatttcagc cgatccaaaa ttgagtcagc cttgctgccc tctttggggt
     5641 tggctatcgg cgtggtcaca tactatctat tcaaggacct gagccccacc atcccggccg
     5701 gactggaatc tggcgccacc ggcgaggtaa attactctag tatgcttgca tggggaatag
     5761 cagctttcgt ccttggggca cccgtcgggc tcttgggcaa cctggcgcga attccaagtg
     5821 ttggcggtct cccattccga ctgattgttc cgctcgtagc acttttcgag acctccatgc
     5881 agctggctgc agaggcggat gggcaacgtg cggtcgttgg aattacttgg aatgtgattc
     5941 ggttcatcgc gggcgctgtt gctttggctc tagtaattca caccatccgg agttggtggc
     6001 actctcggcg tactcgttcc gcggagacgg atgagcacca cgccagaagg gacatttcgt
     6061 ccttgtgacc ttctgaagtc cagcctcgat ccgtcagctg gggccggtcg ttgaggacat
     6121 gaccacgcga gagtcagtgg aatccgcgat tcgcggctgg aacgggctgt ccctgcatcg
     6181 tcgtaccggc gcgtgatcta caacaacgtg tcgacggtcc tcaacgcggc ggtggacgac
     6241 ggtcacctgt cgaagaaccc gtgccacgcg caatcggtgt gtccacctgc tgcgggaagt
     6301 ggccgcgttg tgccgtggag cgccgagcgc gtcttcgctg tacgagcggc actgccagag
     6361 cgataccgcg cgacggtcga cctcggaggc ggatgcggac tccggcaagg ggagatcttc
     6421 gggttgcccg tcgatgcaat cggcttcgac tccggatggc tgcacatcgc gaaccaggtg
     6481 aaggtgacga aagacggtct cgtgttcgcc ccaccgaagc ggaacaagat ccgcgacgtt
     6541 cccttgccga atcgcgtggc gcacgtcctc aagcagcata tggaggcgtt ccccccggta
     6601 gagatcaccc tgccgtggct ccgggtgggc ggtcagagtg tcacgaagcg gctgctcttc
     6661 actcgcctcg acggcgcggg tgcagttcgg cgtactgact tcaacgaccg cgcgtggaag
     6721 cctgctctcg tggccgctgg ggtgatcccc gcgccgaatc cgggagagcg ccaccaggct
     6781 gcccgcgaac acggcatgca cgctctcagg cacttctacg cctcggccct cctggacacc
     6841 ggcgagaacg tcaaggcgct gagcaactac ctcggacaca acgatccggg gttcacgctc
     6901 cgggtctaca cgcacctcat gccgagcagc gacgcacgag cccggaaggc cgtggacggc
     6961 ctgtacgagg gcaccgatcc ggcgcccgac ggcccagaga cggcccaggg tctgtgagac
     7021 gccatgaggc cgacggtccg caaccgtcgg cctcatccgc tgaatctgcg gagaagtcgc
     7081 tctgacctgc gtctacaagg ccggaaggtt cttccgcagc tcgaacgccg tgacctccga
     7141 gcggtactcc tcccactccg acttcttgtt ccgaaggaag aagtcgaaga cgtgctcccc
     7201 gagcgtctcg gcgaccaggt cgctgcgttc catcagggtc agggcctcgc cgaggttctg
     7261 ggggagcggc tcgatgccca tcgcgcggcg ctcggcgtcg gagagggccc agacgtcgtc
     7321 ctcggcgccc ggcgggagct cgtagccctc ctcgatgccc ttcaggccgg cggccaggag
     7381 gacggcgtag gacaggtacg ggttcgcgcc ggagtccagg gagcggacct cgacccgggc
     7441 cgagcccgtc ttgccgggct tgtacatcgg gacgcggacc agcgccgagc ggttgttgtg
     7501 gccccagcag atgtacgagg gggcctcgcc gcccgcgccc gccgtgcgct ccgcgccgcc
     7561 ccagatgcgc ttgtaggagt tgacccactg gttggtgacg gcggagatct ccgccgcgtg
     7621 ccgcagcagg cccgcgatga aggagcggcc caccttcgag agctggtact ccgagcccga
     7681 ctcgtagaag gcgttgcggt cgccctcgaa gagggagagg tgggtgtgca tgccgctgcc
     7741 ggggtgctcg gagaacggct tcggcatgaa ggtcgcctgg acgccctgct ccagcgccac
     7801 ctgcttcatg accaggcgga acgtcatgat gttgtcggcc gtggagagcg cgtcggcgta
     7861 gcgcaggtcg atctcctgct ggcccggcgc gccctcgtgg tgggagaact cgaccgagat
     7921 gcccatggac tccagcatgg tgatcgcctg gcggcggaag tccatgccga cgttggtggg
     7981 ggtgtggtcg aagtagccgg agttgtcggc gggcgtcgga cggctgccgt ccagcggccg
     8041 gtccttcagc aggaagaact cgatctccgg gtgcgtgtag aacgtgaagc ccaggtccga
     8101 ggtgcgggcc agggcgcgct tgagtacgta gcgcgggtcg gcgaaggacg gcgagccgtc
     8161 cggcatgagg atgtcgcaga acatgcgcgc cgtgcccggg gcctccgcgc gccagggcag
     8221 gatctggaag gtcgacggat ccggcttggc gatcatgtcg gactcgtaga cccgggcgaa
     8281 gccctcgatc gcggagccgt cgaagccgat gccctcgtcg aaggcctgtt ccagttcggc
     8341 cggggccacg gcgacggact tgaggaagcc cagcacgtcc gtgaaccaca ggcgtacgaa
     8401 ccggatgtcg cgctcctcca acgtccggag cacgaactcc tgctgcttgt ccatcttccg
     8461 cttccccatc cttgctggtc aggccgcctg tcttccgtag ctcgggaggc ggtcgggcac
     8521 ctgagcatcc caccacacca ccatttcgta cgcgttgcgc accatgatcg cccgggcggc
     8581 ccggggccga acgcctcacg tacggcgggt ggactgctct tccgcccata gtgcctgctc
     8641 gcaccggcat ccgtaacggc cggttcggtt cgctgtcctc ccacttaatt tgcatttcat
     8701 atgcaacttt aaatacggtg cgcggcagtc gagtcctgag gagtcccgat gctgtccgag
     8761 cagtccgccg tcaccgtccg cgccaccctc cccgccgtcg gcgcggccct cggcacgatc
     8821 accgagcgct tctacgccgg gctgttcgcg gcccaccccg agctgctgaa cgacctgttc
     8881 aaccggggaa accaggccgc cggcacccag cggcaggcgc tcgccggatc catcgccgcc
     8941 ttcgcgacgc acctgctcga ccacccggag cagcggccgg acgcgatgct gcaccgcatc
     9001 gcccacaagc acgcctccct caacatcacg cccgagcagt acgacatcgt ccacgagcac
     9061 ctgttcgccg ccatcgccga cgtgctcggc gacgccgtca cccccgaggt cgccgccgcc
     9121 tgggacgagg tctactggct gatggcgaac gccctcatcg ccgtcgagaa gcggctgcgg
     9181 gaggagcgtg gcgggtacgc ctggcgggcc tgggaggtcg tcgagcgcgt cgccgagacc
     9241 gatgacgtcg ccacgttccg gctgcggccc gccgacggcg gtccggtgcg ggacttcctc
     9301 gcgggccagt acgtctccgt ccgcgtcgcg ctggcggacg gcgcccggca gatacggcag
     9361 tacagcctct ccggtgcgcc cgccccggac ctgcggcaga tcagcgtgaa gcgcgtgcac
     9421 ggcgacggca cccccgacgg cgaggtctcg aaccacctcc acgcgcgcgt gcaggcgggt
     9481 gacgtcctgg agctgtccga gccctacggc gacctcgtgc tggacgccgg ccccgacacc
     9541 ccgctgctgc tggcctcggc gggcatcggc gtgaccccca tgaccgcgat gctggcccac
     9601 ctcgccggct cggggcaccg cgccccggtc accgtcgtgc acggcgaccg ctcacccgcc
     9661 gcccacgccc tgcgcaccga ccacgaggcc tacgccgcca agctgcccga cgcgtccgtc
     9721 cacttctggt acgagcagga cgccccggcg ggcacccgct ccggcctggt cgacctcgcc
     9781 gacgttccgg tcgcgccggg cacgcgcgcg tacctgtgcg ggccactgcc cttcatgcgg
     9841 gcggtacggg cccagctgat cggcaagggc gtggcaccgg ccgacatcca ctacgaggtg
     9901 ttcgggccgg acctgtggct ggcggggcag gcggcctaga catcacgggg atcagcgccc
     9961 cgcgagatcc ccagcagcag cgccccggtc ggctccgcca cgatgtccgc caccgtgatc
    10021 gggtccagtg aggcgaagaa cgcctcctgg gcccggcgca gggcgtcgcg caggcggcag
    10081 gcggagttca gggggcaggg cgtggggccc tcgcagtcga cgacgtcgcc gtcgccctcg
    10141 aaggcgcgca ccacggcgcc caccgacgcc gtacggcccc gctcggtgag cgtgagaccg
    10201 ccgccgcggc cgcgacgggc ggccagcaga cccatgtgct ggagctcggc gacgaccttc
    10261 gccaggtggg tgtacg
//
