LOCUS       NZ_CP023702            11044 bp    DNA     linear   CON 23-APR-2024
DEFINITION  Streptomyces nitrosporeus strain ATCC 12769 chromosome, complete
            genome.
ACCESSION   NZ_CP023702
VERSION     NZ_CP023702.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nitrosporeus
  ORGANISM  Streptomyces nitrosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7581562)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008704555.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:20:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,704
            CDSs (total)                      :: 6,606
            Genes (coding)                    :: 6,461
            CDSs (with protein)               :: 6,461
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 66 of 146
            Pseudo Genes (incomplete)         :: 106 of 146
            Pseudo Genes (internal stop)      :: 16 of 146
            Pseudo Genes (multiple problems)  :: 38 of 146
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:19:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7063082
            Orig. end                         :: 7074126
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="26"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6044](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    312..326
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(568..2385)
                     /locus_tag="CP967_RS31460"
                     /old_locus_tag="CP967_31545"
     CDS             complement(568..2385)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP967_RS31460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CP967_31545"
                     /product="hypothetical protein"
                     /protein_id="WP_150491216.1"
                     /transl_table=11
                     /translation="MDNRSLEALGLHVAPRDQPLVYPGAWPVESGLLYRGRLLRLRPGR
                     ARRLAQWLVDSPAEGFGGGTEPGEAVPLDYALMRANEPLAGERFPVLSVGSNACPAQLK
                     HKMDGHGLSSTIPMVKVRTVGIDIGVSAYVSPLGYVSSSPFHAPHVTRDLFVTWFDATQ
                     LEVVDASEGVFTEGGEYDRVLLPGTDFRFELPSGELLGGVYAYVHRYGVLHDGTGTPRP
                     HNGERRLLTELLAESRRLREWFGDTPEQFSSQARGNEQLCEKGTRLFRDEGRTTPSGLE
                     GHVPERPAAVVYDDIQPANPLPAGSHRVARTPDTYDQRGSGVVRLSAGLAADLRHPEHV
                     VLQNAQVPPARRERLGALANVVVAPELDPDDRRTVQVDRSLRICIGVEPGEDIAVRPAA
                     LPRTRRRWRNALFGPLNYVTCRVQDGDRASAEHEVCLLDALTLELLGVSSGDDVVVEGF
                     PGSDGIVPTLQLKAIQTSEEVIERRKDLHGGDLTSRYPSSLDALGTYPDLPWVFLDRRL
                     WAGLGVQGQWLATVRIRCSRTYQLKKELREMMFLLGLAFIGVVTVLESNTWRVISLAVL
                     VLLAGSLVSIRLRSRLTQRARRIGGAARR"
     misc_feature    complement(2451..2468)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            complement(2494..3522)
                     /locus_tag="CP967_RS31465"
                     /old_locus_tag="CP967_31550"
     CDS             complement(2494..3522)
                     /GO_function="GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606976.1"
                     /locus_tag="CP967_RS31465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31550"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_150491217.1"
                     /transl_table=11
                     /translation="MAETARHPEPRYGTRPTMKDVAARAGVGLKTVSRVVNGEPGVTPD
                     TERRVQEAIEALGFRRNDSARVLRKGRTASIGLVLEDLADPFYGPLSRAVEEVARDHGA
                     LLINGSSAEDPEREQELVLALCARRVDGLIVIPAGEDHRYLEPEIKAGVATVFVDRPAG
                     LIEADTVLSDSFGGAREGVAHLLAHGHRRIGFIGDRPHIHTAVERLRGYHAAMADAGID
                     VRESWVSQGATDPGRVAAAAEAMLAGPEPVTALFSGNNRVTVTVVRVLARREDHIALVG
                     FDDIELADLLGVTVVSQDPAAVGRTAAEHLFRRLEGSGRPPARVTLPTRLVARGSGELP
                     PA"
     misc_feature    3615..3629
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.65"
                     /tool="antismash"
     misc_feature    complement(3617..3628)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.52"
                     /tool="antismash"
     gene            3704..4675
                     /locus_tag="CP967_RS31470"
                     /old_locus_tag="CP967_31555"
     CDS             3704..4675
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157754.1"
                     /locus_tag="CP967_RS31470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31555"
                     /product="ROK family protein"
                     /protein_id="WP_150491218.1"
                     /transl_table=11
                     /translation="MTDHLVAALDIGGTKIAGALADDGGSLLVRAQRPTPAEGDAESVM
                     GAVDAVLTELASSPLWPGVGALGIGSAGPVDAAAGTVSPVNVPAWRGFPLVERAGAAVG
                     GLPVVLVGDGVAMTAAEHRLGAARGHDNALCLVVSTGVGGGLVLGGKLHAGPTGNAGHI
                     GHISVDLDGDLCPCGARGCVERLASGPNIARRAVDGGWRPAPGEDATAAAVAAAARAGD
                     PVAIASYERAAQALAAGIAATATLTEIDIAVIGGGVAGAGDVLFAPLRRSLRAYATLSF
                     VRRLTVAPALMGNDAGLVGAAVAAAAVAAGGRSGALRAKAAR"
     gene            complement(5001..6044)
                     /locus_tag="CP967_RS31475"
                     /old_locus_tag="CP967_31560"
     CDS             complement(5001..6044)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677196.1"
                     /locus_tag="CP967_RS31475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31560"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_150491219.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-20, bitscore: 71.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTHGGRQSGSRSVRKVPQHLVHKHDSAEVLLTDWTTHEGDLHT
                     VHARWPRCHTFYQPHCRRFDPLLFVETVRQTFPLLSHAAYGVPFDSHLVWDGFSFEIDP
                     QAMLIPQGRADVVLEAACTDVRTRKGCLASMTMHAEVRLNGRRVGGARTRFTNHSPAVY
                     RRLRGERDLAEARRRALPPLPPLPPATVGRHHHENVVLADSGRADRWQLRADLDHTVLF
                     DHPVDHAPGMLLLEAVRQAAHLNSFTANCMVTGMSVSFEGWVELDVPAWISVAPVGGNV
                     LRAVIEQEHGTCLTAEVVVESVPPRTAGHPAVQNQADLQLSPRLRSAPGPAEAMCSGPG
                     AVVLPVR"
     misc_feature    complement(5496..5498)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5547..5549)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6136..6759
                     /locus_tag="CP967_RS31480"
                     /old_locus_tag="CP967_31565"
     CDS             6136..6759
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677197.1"
                     /locus_tag="CP967_RS31480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31565"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_150491220.1"
                     /transl_table=11
                     /translation="MASVKAERAARTRQKLLLAAAEVFDEAGYEQAAVTQIVERAGFTL
                     GALYFHFGSKQGVAKALMNAQSMTIEPHLQSTGLQRLVDITLVWADRVRRDPILRAGVR
                     LAIEQGSHGMNDATSFEEWRELMRGHLEVARDSGELRDGVCPDRLARFVVGACTGIQVY
                     SNLATEHTDLMERVCDMWGLLLPSVASEAVAHGILVGPVRVPRT"
     gene            6756..7616
                     /locus_tag="CP967_RS31485"
                     /old_locus_tag="CP967_31570"
     CDS             6756..7616
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677198.1"
                     /locus_tag="CP967_RS31485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31570"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_229888290.1"
                     /transl_table=11
                     /translation="MTAPASHVVLTGATGQIASLMVRHLAARHRVTLTSRTPPSAVRPP
                     GVRHVACDYDAYGSVVDAFRGADALFVVTADPMRPEHDRNVVRAAVEVSVKHIVKLSAA
                     AVLDGAAHDLVTTWQRSNEERIKASGIPWTMVQPRSFMSHAFGWAPGIRGRGSTYALYP
                     HSRNACIAPEDIAEVSALLLGDSRHHGRSYQLTGPQALSAHEQSAVLAELLGRPLVCRA
                     LSPGEARLRYLRRYPAAMADALLASARRQLAGAKAHVTDTVERLTGRPATTFAQWAADH
                     RDRFE"
     gene            7857..8027
                     /locus_tag="CP967_RS34220"
     CDS             7857..8027
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CP967_RS34220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167535473.1"
                     /transl_table=11
                     /translation="MLSGTPSFIEPLTGRAVRECLADPVETARAAARLRSDRPSSTTGA
                     VAPAGSGRTAR"
     misc_feature    complement(8550..8559)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.82"
                     /tool="antismash"
     gene            8794..9372
                     /locus_tag="CP967_RS31490"
                     /old_locus_tag="CP967_31575"
     CDS             8794..9372
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="CP967_RS31490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31575"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_150491221.1"
                     /transl_table=11
                     /translation="MKNSERAASTRANLVLAAARHFDQHGYDGTTLNSVCAEVNVTLGA
                     LTFHFRCKAALASAVVQEGVEELRRVSTECSAAGALPELSLLVRQVVTALQRNVLTRAA
                     VRLIQEEHVVSDWLGVFREEVLRLLERASAAGGLFAGVRPDTAVRLVMYVVEGAMAEAR
                     RPADGEGPTGPDFAEVWRAVLNGLAADAR"
     gene            complement(9538..10680)
                     /locus_tag="CP967_RS31495"
                     /old_locus_tag="CP967_31580"
     CDS             complement(9538..10680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677202.1"
                     /locus_tag="CP967_RS31495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_31580"
                     /product="transposase"
                     /protein_id="WP_150491222.1"
                     /transl_table=11
                     /translation="MALPHEPMCGTSAEDDRTPRLPPFIQTVFGSLPRTDQRRWAEFYI
                     RALLRTPGKKSPRRLAESVSDSPTASQALHQFVNASPWDWLTARQGLLRWVAQAGSEVT
                     AWTLVPVFIPKRGDHSAGVHRRFDPAQGRLSNCQLGMAVLATGKYGPLPVDWDLYIPGG
                     WTRTRRAKARVPEEIGGPLWSQASKLLRRAPLQPPGRHAPVTAELSWLPDREALLAHLV
                     RDGRPFVVEVPGATRVARPVPPPAGTPPLTVRQVLRDPATAAGKEQHPLVSLPVVLPGS
                     SAGRVPLRLLVRHRPDGPAQTWLTNLVDRQLPELLPLMRQPRLAASVVGTLNEDFGLQD
                     FEGRSFPGWHHHTTLVSAAFAYSRIDGRGAAGGAGTRSQG"
     misc_feature    complement(10030..10032)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 accggcctgc attacggcac cttcgactac agtgcctgcg tcggcgtcag ccccgcccac
       61 caggccggcg accacccggc cgccgaccac gccaaggcgg tcatgcaggt cgccgcggcg
      121 ggcaccggcg tacgggtctg cgacggctcc accaacgtgc tgcccgtcgg ccccaccgag
      181 caggtccacg acgcctggag gctgcaccac ggcctcaccc ggcgcgccct ggcccgcgcc
      241 tactaccagg gctgggacat gcacccgggg catctgccca cccggtacgc ggccgtctac
      301 gccttctacc gcgagggcct cggccaggcc gcgtcccggc tcgccgccta cgtggccagg
      361 gcgggcggcg acgtcatgga cgagcccgcc accgcgaagg cgctgagcgg ctacctgctg
      421 cgcggcatcg actgcggggc gctggacaac gaggaggtca cccggctcac cggcctcggc
      481 cgcgcggacc tggacgcctt cgcctcaccg cggcgcggcg gcccggcggc ctgagccgcc
      541 gcgggcgggg cgcgggcgtc cggtccctca ccggcgggcc gcgcccccga tccgcctcgc
      601 ccgctgggtg agtctgctgc gcagccgtat gctgacgagg gacccggcca gcagcaccag
      661 aacggccagg ctgatgacgc gccaggtgtt gctctccagc acggtgacca caccgatgaa
      721 ggccagcccg agcaggaaca tcatctcgcg gagttccttc ttcagctggt aggtgcggct
      781 gcagcggatg cggaccgtgg cgagccactg gccctggacg ccgagacctg cccacaggcg
      841 gcggtccagg aacacccacg gcagatcggg gtacgtgccg agggcgtcga gggagctggg
      901 gtaccggctg gtcaggtcgc cgccgtgcag atccttgcgg cgttcgatca cctcctcact
      961 ggtctggatg gccttgagct gcagcgtcgg cacgatgccg tcgctgccgg ggaacccctc
     1021 caccacgacg tcgtcaccac tgctgacgcc gagaagctcc agcgtcaggg cgtccaggag
     1081 gcagacctcg tgctcggcgc tggcccggtc gccgtcctgg acgcggcagg tcacgtagtt
     1141 gagcggtccg aacagggcgt tccgccagcg gcgccgggtg cgggggagcg ccgccggcct
     1201 gacggcgatg tcctcgcccg gctccacccc gatgcatatc cgcagcgacc ggtccacctg
     1261 gaccgtgcgc cggtcgtcgg ggtcgagctc gggtgcgacg accacgttgg cgagcgcgcc
     1321 cagccgctcg cgccgggccg gaggcacctg ggcgttctgc aggaccacgt gctcggggtg
     1381 ccgcaggtcc gccgccagcc cggccgacag ccgtacgacc cccgagccgc gctggtcgta
     1441 ggtgtccggg gtacgggcca cccggtgtga tcccgcgggg agggggttgg ccggctggat
     1501 gtcgtcgtac acgacggcgg cgggcctttc ggggacatgg ccctccagcc cggacggggt
     1561 ggtccggccc tcgtcccgga agagccgggt gcctttctcg cagagctgct cgttgccgcg
     1621 agcctgtgaa ctgaactgtt cgggggtgtc gccgaaccac tcgcgcaggc gccgcgattc
     1681 ggccagcagt tccgtgagca gccgcctctc gccgttgtgg gggcgcggtg tgccggtgcc
     1741 gtcgtggagc acgccgtacc ggtggacgta cgcgtagacc ccgcccagca gttcgccgga
     1801 cggcagctcg aaccggaagt ccgtccccgg gagcaggacc cggtcgtact cccctccctc
     1861 ggtgaagacg ccctcgctgg cgtcgacgac ctcgagctga gtcgcgtcga accaggtgac
     1921 gaacaggtcc cgggtgacat gaggggcgtg gaagggggac gaggacacgt agccgagcgg
     1981 gctcacgtag gcggagaccc cgatgtcgat ccccacggtg cggaccttca ccatggggat
     2041 ggtcgaagag aggccgtggc cgtccatctt gtgtttcagc tgcgccgggc aggcgttcga
     2101 gcctacggac agcacgggga agcgctcacc ggccaacggt tcgttggccc gcatcagggc
     2161 gtagtccagc ggtacggctt cgcccggctc cgtgccgcct ccgaaccctt cggcggggct
     2221 gtccaccagc cactgagcca gtctgcgagc ccggccgggg cgcagccgca acagccggcc
     2281 gcggtacagc agcccggatt ccacaggcca ggcccccgga tagacgagcg gctggtcgcg
     2341 tggtgccacg tggaggccga gcgcttcgag cgatcgattg tccatctggt tcctggcctt
     2401 ccccggatga ttcggcgttc gggcgcagtg cgtccgatag cgtaatgaat caccggctca
     2461 tcgggatagg ccgcttcgga gtgacaacgg ggctcaggcg ggcgggagtt cgcccgagcc
     2521 gcgggcgacg aggcgggtcg ggagcgtcac ccgggccggc ggacggcccg atccctccag
     2581 ccggcggaag aggtgctccg ccgccgtgcg tcccaccgcc gccgggtcct gggagaccac
     2641 ggtgacgccg aggaggtcgg ccagctcgat gtcgtcgaag ccgacgaggg cgatgtggtc
     2701 ctcgcgacgg gcgaggacgc gcacgacggt cacggtcaca cggttgttgc ccgagaagag
     2761 cgcggtgacc ggctccgggc ccgcgagcat ggcctcggcc gccgccgcga cccgtccggg
     2821 gtcggtcgcg ccctgggaga cccaggactc gcggacgtcg atgcccgcgt cggccatggc
     2881 cgcgtggtag ccgcgcagac gctccacggc cgtgtggatg tgaggacggt cgccgatgaa
     2941 gccgatccgg cggtggccgt gcgccagcag gtgggcgacc ccttcacggg caccgccgaa
     3001 gctgtccgag aggaccgtgt cggcctcgat cagacccgcc ggccggtcga cgaagacggt
     3061 cgcgacgccc gcctttatct cgggctccag ataccggtgg tcctcgcccg ccggaatcac
     3121 gatgagaccg tccacccgcc gggcgcagag cgccagtacg agctcctgtt cgcgctcggg
     3181 gtcctcggcg ctggaaccgt tgatcagcag cgcgccgtgg tcgcgcgcga cctcctccac
     3241 ggcgcggctg agcgggccgt agaaggggtc cgcgaggtcc tccagcacca ggccgatgga
     3301 ggcggtgcgg cccttgcgga gcacccgcgc gctgtcgttg cggcggaagc ccagcgcctc
     3361 gatggcctcc tggacgcggc gctcggtgtc cggcgtgacg cccggttcgc cgttcaccac
     3421 gcgggagacc gtcttcaggc ccacccccgc gcgggccgcc acgtccttca tcgtcggcct
     3481 ggtgccgtag cggggctcgg ggtgacgggc ggtctcggcc acgatgcgct gtcctgtcgt
     3541 cgagtgggcg tgcccggccc gctcatgggc ggagccgggg aggtgccgga ggctgtggcg
     3601 tcgagcatag gccctggaca acgttgtcat aagaatggag actgggcggg ccgcggaccc
     3661 ggcgtcgcag gtccgcccca caccctccgg agagtcctga ccgatgaccg accacctcgt
     3721 cgccgcgctg gacatcggcg gcaccaagat cgccggcgcg ctggcggacg acggcggatc
     3781 acttctcgtg cgggcccagc ggccgacacc ggcggaaggg gacgccgagt cggtcatggg
     3841 ggcggtggac gccgtcctca ccgagctggc gtcctccccg ctgtggcccg gggtgggcgc
     3901 gctcggtatc ggcagcgcgg gtcccgtgga cgcggcggcg ggaacggtca gcccggtcaa
     3961 cgtccccgcg tggcgcggct tcccgctggt ggaacgggcc ggtgcggccg tcgggggact
     4021 gcccgtcgta ctggtcggcg acggcgtggc catgacggcc gccgagcacc ggctgggtgc
     4081 ggcccgaggg cacgacaacg cgctctgcct ggtcgtctcc accggtgtcg ggggagggct
     4141 ggtgctcggc gggaagctgc acgccggccc cacgggcaac gccggccaca tcggccacat
     4201 cagcgtcgat ctggacggcg acctctgccc gtgcggcgcc cggggctgcg tcgaacgcct
     4261 cgccagcggg cccaacatcg cccgccgggc cgtggacggc ggctggcgcc ccgctcccgg
     4321 cgaggacgcc acggcggccg ccgtggccgc ggccgcccgg gccggcgacc cggtcgccat
     4381 cgcctcgtac gaacgtgcgg cacaggcgct cgccgcgggc atcgccgcga ccgcgaccct
     4441 caccgagatc gacatcgcgg tgatcggcgg aggggtggcg ggcgcgggtg acgtactctt
     4501 cgcgccgctc cgcaggtcgc tgcgggcgta cgccaccctc tccttcgtcc ggcggctcac
     4561 cgtggccccg gccctcatgg gcaacgacgc gggtctcgtc ggcgcggcgg tggcggccgc
     4621 cgcggtggcc gccggcggcc gttccggggc cctgcgggca aaggccgcca ggtaacccgc
     4681 cgggtagctg tccggctgcc gtgcccgccg ggctgccgtg cgccccggag cgcacggcag
     4741 cccggcggaa ccgcaccgcc ccgacgggat ttccccggca tggtgtcgcg ggcaagccgc
     4801 agggggctga ggaagggggg cccgtgaccg tccggaccga ctgcgcgtcc tggagcctgt
     4861 ccgacgggtc aggagcagag gcgcccactc aggccgccat gaggcgcttt gtgccccggt
     4921 gatggactgg acgcggatgc gaggctgcta cggggacacg gggatacgag gcgcggaaac
     4981 ccgcttgagc ggccggagcg tcatctgacc ggcaggacga ccgctccggg cccggaacac
     5041 atcgcctccg ccgggcccgg agcgcttcgg aggcggggcg acaactggag gtccgcctgg
     5101 ttctgaacgg ccgggtgtcc cgccgtccgg ggcggcacgg actcgaccac gacctcagcg
     5161 gtcagacagg tgccgtgctc ctgctcgatg acggcccgca gcacgttgcc gcccaccggt
     5221 gcaaccgaga tccaggcggg gacgtccagt tccacccagc cttcgaagga gacggacatt
     5281 ccggtcacca tgcagttcgc ggtgaaggag ttcagatggg cggcttggcg gacggcttcc
     5341 aggaggagca tgccgggagc atggtcgacc gggtggtcga acagtacggt gtggtccagg
     5401 tcggcgcgta gctgccatcg gtccgcgcgt ccgctgtccg ccaggaccac gttctcgtgg
     5461 tggtggcgtc cgaccgtggc gggcggtagc ggaggtaagg ggggcaaggc ccggcgacgg
     5521 gcctcggcga ggtcgcgctc gccgcgtaat cgccggtaga cggcggggga gtggttggtg
     5581 aaccgggttc tggcgccgcc gacgcgacgg ccgttcagcc ggacctccgc gtgcatggtc
     5641 atcgaggcca ggcagccctt tctggtccgg acgtcggtgc aggcggcctc caggaccacg
     5701 tcggcccggc cctgcgggat cagcatggcc tgcggatcga tctcgaagga gaagccgtcc
     5761 cagaccagat gactgtcgaa gggcacgccg taggcggcgt gggacagcaa cgggaacgtc
     5821 tgacgcaccg tctcgacgaa gagcaagggg tcgaagcgcc ggcagtgggg ctggtagaag
     5881 gtgtggcaac ggggccaccg ggcgtggacg gtgtggaggt ctccctcatg ggtggtccag
     5941 tcggtgagga gcacctcggc cgaatcgtgt ttgtggacga ggtgctgagg gaccttcctg
     6001 acgcttcgtg acccgctctg tctgccaccg tgagtgttgg acatgcagca ctacccccct
     6061 gaatcattgg cccgggtgac ggccgttcag ctaacataaa ttacgttatc tatgtctgtc
     6121 gaggagtggt tcagggtggc atctgtcaag gcggaacggg ctgctcgtac gcggcagaaa
     6181 ctcctgctgg cggcggctga agtcttcgac gaagccggtt acgagcaggc cgcggtgacc
     6241 cagatcgtcg aacgggcggg attcaccctc ggtgccctct acttccactt cggctcgaag
     6301 cagggggtgg ccaaagccct gatgaacgcc cagtcgatga cgatagaacc ccacctccag
     6361 tcgaccggac tgcagcgcct ggtcgacatc accctcgtgt gggcggaccg tgtgaggcgc
     6421 gatccgatcc tgcgtgcggg agtccgcctc gccatagagc agggcagcca cggcatgaac
     6481 gacgccacct ccttcgagga gtggcgggag ctcatgcgcg gccatctcga ggtggcccgg
     6541 gacagcggtg aactccgcga cggggtctgt cccgaccggc tggcgcgttt cgtggtcggc
     6601 gcctgcaccg gcatacaggt ctactcgaac ctggcgaccg aacacaccga tctgatggag
     6661 agggtctgcg acatgtgggg acttctgctg cccagcgtcg cgtccgaggc ggtggcgcac
     6721 ggcatcctgg tcggccccgt acgtgtcccg agaacgtgac cgcacccgcg tcccacgtcg
     6781 tgctcaccgg ggccacaggg cagatcgcct cgctgatggt ccggcacctc gcggctcgcc
     6841 accgagtcac cctcaccagc cgtactccgc cttccgcggt gcggcctccc ggtgtccggc
     6901 atgtcgcctg cgactacgac gcgtacggga gcgtcgtcga cgcgttccgc ggtgctgacg
     6961 ccctcttcgt cgtgaccgcc gaccccatgc gtcccgaaca cgaccggaac gtggtgaggg
     7021 ccgcggtcga ggtctccgtg aagcacatcg tgaaactctc cgcggcggcg gtgctcgacg
     7081 gggccgcgca cgacctcgtc accacgtggc agcggagcaa cgaggagcgc atcaaggcga
     7141 gcggcatccc ctggaccatg gtccagccgc ggtcgttcat gtcccacgcc tttggatggg
     7201 cgcccggcat ccgcggccgc ggctccacgt acgccctgta cccccacagt cgcaacgcct
     7261 gtatcgcgcc cgaggacatc gcggaggtct ccgctctcct cctgggggac agcaggcacc
     7321 acggccggtc gtaccagctc accgggccgc aggcgctctc ggcccacgag cagtcggcgg
     7381 tccttgccga actgctgggg cgccccctcg tctgccgggc gctttccccc ggggaagccc
     7441 gcctgcgcta cctgcggcgg tacccggcgg cgatggccga tgccctgctc gcgagcgcca
     7501 ggcggcagct cgccggtgcc aaggcccacg tcaccgacac ggtcgagcgt ctcaccgggc
     7561 ggcccgccac caccttcgcg cagtgggcgg ccgatcaccg ggaccgcttc gagtgagacc
     7621 gggcacccgc cgggcacggg tgccgggtgc tccccggacc gcgcggcccg gccctgtcat
     7681 gtgaccggtg ggggcgctgt gcggtgatca gcggaacagg atccgccggc ggagcagggg
     7741 gatcctgttc cgccgcgcgt ctgcctcatg atcttcttgg tccgcgtgcc ggcgcccccg
     7801 acgcgagcgc tgcggcggga ggcgggtccg gcgtcgacgg cggcacgacg ggccgggtgc
     7861 tctccggcac gccctcattc atcgagcccc tcaccggacg cgccgtccgg gagtgcctgg
     7921 ccgatcccgt cgagaccgcc cgggcggccg cacggctccg ctcggaccgc ccctcgtcga
     7981 ccaccggcgc cgtcgccccg gccggcagcg gacggaccgc ccggtgaacc gcaccgcacc
     8041 ggcgaacttg cccggaacgt tccgggtttc cttcgttccc gtgctcatca ccgacgccca
     8101 ccgtagggtc ggacccccgt cgagccgctg ccgcgggacc gcgccacccg ctgccacggg
     8161 cagccagagc tgaggacggt acgtgaggga cctggctggc ctcgacatca cacgggtcaa
     8221 ccgcggtgtg gcggagggcg ggagaggaac cggacggtcc ggcggccctg ttccccacgg
     8281 aagggccagg tcctgttcgc gggccgtcgg accccctccc gcccccggcc gggagggaca
     8341 cgacgccacc ggtgactccg gggtcctccg cacccggccc gcacgtccct tgccgccaca
     8401 ggcgcggccc ggaccgttcg ggacgtgcgg tccggccggc ccggccccgg aggcggccca
     8461 ccggcccggc gggccggccc cgcaccgtca tgccgcgggc gcggcggtct tgtggcggcg
     8521 ccggggccct gccaccaccg agaggacgct caaagaacgc cgggcggaga ggcgcgttcc
     8581 ttccccgcgg cccccgcggt gcacggcccg catccgccgc tccccaccgg cgtctccacc
     8641 ggccgcgcgc cctgccctgc cccgcacaac tggcgccctc tgatccggcc cggcctccga
     8701 agggggccgg tccggtccgg ccggcccgtc cggagcccgc ttccggcgtg ccggctgtgc
     8761 gccctcattg ccgtcgcgat ccccgaggac ttcatgaaga acagtgaacg agccgcaagc
     8821 acccgcgcga acctcgtcct ggcagcagca cgccacttcg accagcacgg ttacgacggt
     8881 acgacgctca acagcgtgtg cgcggaggtg aacgtcacac tcggggccct cactttccac
     8941 ttccgctgca aggcggcgct cgcgtccgcg gtggtgcagg agggcgtcga ggaactgcgg
     9001 cgcgtcagca cggagtgctc cgccgccgga gcccttcccg aactgtccct cctcgtccgg
     9061 caggtggtga cggccctcca gcgcaacgtc ctcacccggg ccgccgtacg cctgatccag
     9121 gaggagcacg tcgtctccga ctggctcggc gtcttccgcg aagaggttct gcgcctcctc
     9181 gaaagggcat cggccgccgg tggcctcttc gccggtgtac ggccggacac cgccgtgcgt
     9241 ctcgtcatgt acgtggtgga gggggccatg gccgaggcca ggaggcctgc ggacggagaa
     9301 ggcccgacgg ggcccgactt cgccgaggtc tggcgcgccg tgctcaacgg tctggctgca
     9361 gacgcgcgct gagcacccgc tgtcggcatg tgcccccggg aacacggccg ccggccgtca
     9421 ggcaggtgtg tgaggcgggc cgccgcgcac gggggaggag tcgagggtgc ggcccgtggc
     9481 ctcctcgtac ggccggcctc tgtgtgcgac cggtcgggcg gtgggcgttc caaggcctca
     9541 gccctgggag cgggtcccgg caccgcccgc tgcaccgcgg ccgtcgatcc gggagtaggc
     9601 gaaggcggcg gagaccagcg tcgtgtggtg atgccacccc gggaacgacc ggccttcgaa
     9661 gtcctggagc ccgaagtcct cgttcagggt gcccaccaca ctcgcggcca gccgtggctg
     9721 gcgcatcagc ggcagcagtt cgggcagctg ccggtcgacc agattcgtca gccacgtctg
     9781 cgccggaccg tccgggcggt gccggacgag cagccgcagc ggaacgcgtc ctgcggatga
     9841 gccgggcagg accaccggga gggatacgag ggggtgctgt tccttccctg ccgcggtcgc
     9901 gggatccctc aggacctgcc ggacggtgag gggcggtgta ccggccggcg ggggaaccgg
     9961 acgtgcgaca cgtgtggcgc cgggaacctc caccacgaag ggccgcccgt cccgtaccag
    10021 atgagccagt aaggcttccc ggtccggcag ccaggacagt tcggccgtga caggggcgtg
    10081 ccggccggga ggctgcaagg gggcccggcg cagcagcttc gaggcctgtg accacaaagg
    10141 gccgccgatc tcttccggca cccgcgcctt ggcccgccgg gtcctcgtcc acccgccggg
    10201 gatgtacagg tcccagtcga ccggcagggg accgtacttg cccgtggcca gcacagccat
    10261 tcccagctgg cagttcgaca gccgcccctg cgcggggtcg aacctgcggt gcactccggc
    10321 ggagtggtcg ccgcgcttgg ggatgaacac cggtaccagc gtccaggccg tcacctccga
    10381 gccggcctgc gccacccagc gcagcaggcc ctgacgtgcc gtcagccagt cccacgggct
    10441 cgcgttcacg aactggtgca gagcctggga ggcggtaggg gagtcgctga cggattccgc
    10501 cagccggcgc ggcgacttct tgcccggtgt ccgcaggagc gccctgatgt agaactccgc
    10561 ccacctgcgt tgatccgttc gggggaggga gccgaagacg gtctggatga agggggggag
    10621 gcggggagtc cggtcgtctt cggcggacgt tccgcacatc ggctcgtgcg gcagtgccat
    10681 cggggcttcc tttccacgtg acgtaccgct gtcccgctgt ccctggtccg agtgagggcg
    10741 ttgccgcctc ctggaggacg ggggtcccca ggaggcgggg ccgagctaag cataaagagc
    10801 gctatttacg tgtgttgcgg cgacgagcgt aacgcgactt cggcacctcc accggtcgcc
    10861 cccttccgcg gtcacggaag ggggcgcgga ctcgccgggg tcatccgcgc tgcctccagg
    10921 agcggggccc gcccggggag cgggcttcac cgggcgggag ttcgggcccc cgggggaccc
    10981 gtgtggcacc ggccgaactc cccgcccgcc ggcggacagc cgccccggaa accggggcgc
    11041 gact
//
