LOCUS       NZ_CP023702            10399 bp    DNA     linear   CON 23-APR-2024
DEFINITION  Streptomyces nitrosporeus strain ATCC 12769 chromosome, complete
            genome.
ACCESSION   NZ_CP023702
VERSION     NZ_CP023702.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nitrosporeus
  ORGANISM  Streptomyces nitrosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7581562)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008704555.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:20:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,704
            CDSs (total)                      :: 6,606
            Genes (coding)                    :: 6,461
            CDSs (with protein)               :: 6,461
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 66 of 146
            Pseudo Genes (incomplete)         :: 106 of 146
            Pseudo Genes (internal stop)      :: 16 of 146
            Pseudo Genes (multiple problems)  :: 38 of 146
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:19:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6095280
            Orig. end                         :: 6105679
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(1947..2960)
                     /locus_tag="CP967_RS26925"
                     /old_locus_tag="CP967_27005"
     CDS             complement(1947..2960)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104671.1"
                     /locus_tag="CP967_RS26925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27005"
                     /product="VOC family protein"
                     /protein_id="WP_150490448.1"
                     /transl_table=11
                     /translation="MTSLVQHITIDCSDPYRLALFWAGVLGSSVSGEDAPGDPEALVEA
                     PGAALLFVMVPEPRHGKNRVHLDLRPEDRTRDAEVERLLELGAALVADHRKPNGRGWVT
                     LADPEGNEFCVECGTAERALLTGARLPVTADDVDLAVRLVVDTFAAAAGRDWRVPAGGL
                     AWDCWETAEHLGDDLFAYAVQLGPRRPPQDREVAFRYAPDREGGPANSVFADPSAGVAG
                     LLETVEASGALLAAMVRTASPDLRAHHVFGVSDPEGFAAMGVVETLVHAYDIARGLGLE
                     WAPPGDLCGRVLARLFPDAPDDADRWTVLLWSTGRADLPGRERLTTWRWYGAPPTG"
     gene            3116..3949
                     /locus_tag="CP967_RS26930"
                     /old_locus_tag="CP967_27010"
     CDS             3116..3949
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044101.1"
                     /locus_tag="CP967_RS26930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27010"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_150490449.1"
                     /transl_table=11
                     /translation="MILVTGATGNIGRALVRELSASGAGPTGGLTRDTARAGFPEGVEA
                     VEGDLARADSLEPVLRGVRSLFLVSRVGEDAGIIRTARQAGVEHVVLVSSITVRTHPHL
                     GPARENADVERLLRDSGMAWTVLRPTQFASNALWWAETIRERGTVRAPYADTGLPTVHP
                     ADIASVARAALTGPGHHGRTYELTGPERITARQQAAAIAEVLGREVPFAEAGRQEAHRR
                     MAAFMGGEAADAVLDLMGGDVNDELLAVRDTVARITGARARTFRQWAAENAADFR"
     gene            complement(4101..4997)
                     /gene="thpD"
                     /locus_tag="CP967_RS26935"
                     /old_locus_tag="CP967_27015"
     CDS             complement(4101..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="CP967_RS26935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27015"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_150490450.1"
                     /sec_met_domain="PhyH (E-value: 3.4e-37, bitscore: 127.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAEARTDLYPTRGAAEMTTPRQDPVIWSAPGAPGPIAAKDLQS
                     FEHDGFLAIDQLITPDEVTGYVAELDRLIADPAVRADERSIIEPKSQSVRSVFEVHRIS
                     EVFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEAL
                     TKMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENTAQEPFAA
                     PIRRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="CP967_RS26940"
                     /old_locus_tag="CP967_27020"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="CP967_RS26940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27020"
                     /product="ectoine synthase"
                     /protein_id="WP_150490451.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.5e-53, bitscore:
                     177.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHIKSASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWISPGTMYLLDGHEHHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5603..6865)
                     /gene="ectB"
                     /locus_tag="CP967_RS26945"
                     /old_locus_tag="CP967_27025"
     CDS             complement(5603..6865)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2e-70.
                     Score: 229.4. Matches aSDomain:
                     nrpspksdomains_CP967_RS26945_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="CP967_RS26945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27025"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_150490452.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.1e-75, bitscore:
                     251.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALLDYIERDGITHGLDMATTAKRAFLETFENVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKGELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICGEEAEAQFRGRGLVWGLEFTDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ISPEELDEGLRTLARSVRETA"
     aSDomain        complement(5777..6781)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP967_RS26945_Aminotran_3.1"
                     /evalue="2.00E-70"
                     /label="CP967_RS26945_Aminotran_3.1"
                     /locus_tag="CP967_RS26945"
                     /protein_end="363"
                     /protein_start="28"
                     /score="229.4"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALLDYIE
                     RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICGEEAEAQFRGRGLVWGLEFT"
     gene            complement(7022..7549)
                     /gene="ectA"
                     /locus_tag="CP967_RS26950"
                     /old_locus_tag="CP967_27030"
     CDS             complement(7022..7549)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="CP967_RS26950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27030"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_268253016.1"
                     /transl_table=11
                     /translation="MTAAPADIARARTEFLTIDTPRVEDGAAIWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARDEKGEPVAFVTGYFRPDRPGTLVVWQVAVDHAHRGRGLAAEL
                     LDALTSRVAAERGLDAVETTVTPDNTASDRLFTSYAQRHDVPLEREVLFDGGLFPEGTH
                     LPEVLYRIGPFA"
     misc_feature    complement(7568..7577)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.08"
                     /tool="antismash"
     misc_feature    7676..7692
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.3"
                     /tool="antismash"
     misc_feature    7680..7694
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.31"
                     /tool="antismash"
     misc_feature    7681..7694
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.4"
                     /tool="antismash"
     misc_feature    complement(7705..7720)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7715..7730
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7715..7732)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7845..7859
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.11"
                     /tool="antismash"
     gene            complement(7910..9016)
                     /locus_tag="CP967_RS26955"
                     /old_locus_tag="CP967_27035"
     CDS             complement(7910..9016)
                     /NRPS_PKS="Domain: Aminotran_5 (18-291). E-value: 2.5e-27.
                     Score: 87.6. Matches aSDomain:
                     nrpspksdomains_CP967_RS26955_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607777.1"
                     /locus_tag="CP967_RS26955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27035"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_150490453.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.7e-21, bitscore:
                     74.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTAARFAAIERRVAGLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLAVPFHTAVTAAQVEEALAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERMESNPRAPRRSYLSLLDWKARWIDPGRTALPHAP
                     AQLEMLALEACVERIEAEGLDTLTARHAAAAAATRAGALALGCGLGPYVHDARDAAPVA
                     TTLRTPAGVDASALVARALAADPSLPLIAGGGALSSEMIRVNHYGVDATRGAVLSSLSA
                     LGSALADTGRPADLDAARKAVSETWPGG"
     aSDomain        complement(8144..8962)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP967_RS26955_Aminotran_5.1"
                     /evalue="2.50E-27"
                     /label="CP967_RS26955_Aminotran_5.1"
                     /locus_tag="CP967_RS26955"
                     /protein_end="291"
                     /protein_start="18"
                     /score="87.6"
                     /tool="antismash"
                     /translation="IERRVAGLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNVVT
                     GPYGQTFGNWLRDCGAEVVDLAVPFHTAVTAAQVEEALAAHPEIDFVSLVHAEAATGNT
                     NPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVSV
                     SARAWERMESNPRAPRRSYLSLLDWKARWIDPGRTALPHAPAQLEMLALEACVERIEAE
                     GLDTLTARHAAAAAATRAGALALGCGLGPYVHDARDAAPVATTLRTPAGVD"
     gene            9135..10235
                     /locus_tag="CP967_RS26960"
                     /old_locus_tag="CP967_27040"
     CDS             9135..10235
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459504.1"
                     /locus_tag="CP967_RS26960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_27040"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_150490454.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3e-18, bitscore:
                     64.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHEPARTLRITGRVLLGPDDVRDEVWAVGGRITHERPPGADDA
                     LTLTGWALPGLVDAHCHVGLDKHGPVDAATAEAQALTDRDAGALLVRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAHVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEDSLRDLAEAGIDCVEHATGLTEDTV
                     PLFAERGVAIVPTLVNIATFPGLAADGEAKFPRWSAHMRRLHARRYDTVRAAYDAGIEV
                     YVGTDAGGSLAHGLVAAEVAELVKAGIPPLEALSATTWGARRWLGRPVLEEGAPADLVL
                     YDEDPRADVRVLAGPRRVVLNGRVIG"
ORIGIN
        1 gctggaaccc gacccgggcc gctacgactt cggctggctc gaccgcctgc tggacctcct
       61 gcacgtcaac ggcgtccgcg ccgacctcgg cacccccacc gtcgtaccgc cggcctggtt
      121 ctaccgcgcc caccccgagg ccctcccggt cggcaaggac ggcgtccggt acgccttcgg
      181 ggcgcgcggc gcgatctgcc acagctccac cgcctaccgc gaggccgccg ccggcatcac
      241 cgaacagctg gcccgccgct acgcgaacca cccggccctc gctatgtggc acgtccacaa
      301 cgagtacggg gtcccggtca gcgcctgcta ctgcgacagc tgcgccgccc acttccgccg
      361 ctggctcacc gcccgccacg gctcggccga cgccgtcaac gaggcatggg ggaccgcctt
      421 ctggggccag cgctaccgca gcctcgacga gatcgaaccg ccccgcaccg cccccacggt
      481 cggcaacccg gcccagcagc tggactacgc ccgcttcgcc gacgccacca tgcgcgagaa
      541 cttcaccgcc gaacgcgaca tcctgcaccg gctctcgccc ggcatccccg tcaccaccaa
      601 cttcatgacc gcgctcagcc agtgcgagtc cgtcgactac tgggcctggg gccgcgaggt
      661 cgacctcgtc accaacgacc actacctcat caccgacggc cgccgcaccc acgtcaacct
      721 cgccatggcc gccgacctca cccgctcggt ggccggcggc gccccctggc tcctgctgga
      781 gcactccacc agcggcgtca actggcagcc ccgcaacccc gccaagcgtc ccggcgagat
      841 ggcccgcaac agcctcgccc acatcgcccg cggctccgag ggcgccatgt tcttccagtg
      901 gcggcagtcc cggcgcggcg ccgagaagtt ccactccgcg atggtccccc acgccggcac
      961 cgactcccgg gtgtggcgtg aggtcagcgc gctcggcgcg gacctcggcc tgctcgaagg
     1021 cgtccggggc acccggaccg tcgccgacgt cgccatgctc tgggactggc agtcctggtg
     1081 ggcgcagtcc ctggactggc gccccagcga ggaccacgac gcccgcgaac gcgccgacac
     1141 cttctacgcc tcgctctacg accaccacct caccgtcgac ttcgcccacc ccgacgccga
     1201 cctgtccgcc taccccctgg tcgtcgtccc cgctctctac ctcgccaccg aggagaccgg
     1261 ccgcaacctg cgccgctacg tcgagcgggg cggcaccctc gtcgtctcgt acttctccgg
     1321 catcgtcgac gccgacgacg ccgtgcaccc cggcccctac cccggcgccc tgcgcgacgt
     1381 cctgggcctg acggtcgagg agttctcccc gctcctcgcc ggcggcacgg tccgtctgac
     1441 gacccccgaa ggcgcccccc agggccccgg actcaccggg gacgtctggt cggacgtgat
     1501 cgtcccgcgc ggtgccgaga cctgctggtc gtacgccgac ggcgtccccg ccggccgccc
     1561 cgcgatcacc cgcaaccggc tgggcgaggg cacggcctgg tacatctcca cccggctgag
     1621 cggcccggac ctggacgccg tgctggaccg cgccctggcc gacgcccgga tcgcaccgcg
     1681 cacctcgctc gcccgcgacg tcgaggccgt acgccgtacc ggggagagcg gcagctacct
     1741 cttcctgatc aaccacaccg gcaccgcggc ggaggtaccg ctggagaccc ccggcaccga
     1801 actcctcacc ggtgagcggg ccgggaagga gtgcaccgtc ccggccggtg ccgtccgcgt
     1861 cgtacgcctc gacggctgag ccgccggacc gcgcacacgg ggccgccccc ggaggtgaga
     1921 ggccgcggcc cccgcccgca gtaccttcag cccgtcggcg gggcgccgta ccacctccac
     1981 gtggtgagcc gctcccggcc cggcaggtcc gcacggccgg tggaccacag cagcacggtc
     2041 caccggtccg cgtcgtccgg ggcgtccggg aagagccggg ccagcacccg gccgcacaga
     2101 tccccgggcg gcgcccactc cagccccagc ccccgggcga tgtcgtacgc gtgcaccagc
     2161 gtctccacca cccccatcgc cgcgaacccc tcggggtccg acaccccgaa cacgtggtgc
     2221 gcgcgcaggt ccggtgaggc cgtgcgcacc atcgccgcga gcagggcacc gctcgcctcc
     2281 acggtctcca gcagccccgc gaccccggcg gacgggtcgg cgaagaccga gttcgccggc
     2341 ccgccctcac ggtccggcgc gtacctgaac gccacctcac ggtcctgcgg gggcctgcgc
     2401 gggccgagtt gcacggcgta cgcgaacagg tcgtcgccca ggtgctcggc ggtctcccag
     2461 cagtcccacg ccagcccgcc ggccggtacc cgccagtccc ggccggcggc ggccgcgaac
     2521 gtgtccacca cgagccgcac cgcgaggtcc acgtcatcgg cggtcaccgg cagccgtgcc
     2581 ccggtcagca gggcccgctc ggccgtcccg cactccacgc agaactcgtt gccctccgga
     2641 tccgccaggg tcacccagcc ccgcccgttc ggcttgcggt gatcggcgac cagggccgcg
     2701 ccgagctcca gcagccgctc cacctccgcg tcccgggtgc ggtcctcggg ccgtaggtcc
     2761 agatggaccc ggttcttgcc gtgcctgggc tcgggcacca tgacgaacag cagcgcggcc
     2821 cccggcgcct ccacgagtgc ctcgggatca ccgggcgcgt cctccccgga cacggaggag
     2881 ccgagcaccc cggcccagaa gagggccagc cggtaggggt cggagcagtc gatggtgatg
     2941 tgctgcacga gagacgtcat ggaggccact gttccctccg gccgcgggcg cggtccagcg
     3001 gattgtccgc gcgcgagcgg gccggggcgc cccggaggac tccttgacct gaagcaaggt
     3061 cgaggtccta gcgtcacggt gtaccggcta ccgcccgacg acaggagaag accccgtgat
     3121 cctcgtgacc ggagccaccg gaaacatagg aagagccctc gtacgggagc tgtcggcgtc
     3181 cggcgccggg ccgacgggag gactcacccg cgacaccgca cgggccgggt tccccgaagg
     3241 ggtcgaagcc gtcgagggcg acctcgcccg cgcggactcg ctggaacccg tgctgagggg
     3301 ggtgcggtcg ctgttcctcg tgtcacgcgt gggcgaggac gccgggatca tccggaccgc
     3361 ccggcaggcg ggcgtggagc acgtggtgct ggtctcgtcc atcaccgtcc ggacccatcc
     3421 gcatctgggc cccgcgcgcg agaacgcgga cgtcgagcgg ctgctcaggg acagcggcat
     3481 ggcgtggacg gtcctgcggc ccacgcagtt cgcctcgaac gcgctgtggt gggcggagac
     3541 gatccgggaa cgggggacgg tccgcgcacc ctacgccgac accggcctcc ccacggtcca
     3601 ccccgcggac atcgcctcgg tggcccgggc ggcactgacc gggcccggcc atcacgggcg
     3661 gacgtacgaa ctgaccggcc cggagcggat cacggcccgg cagcaggccg cggccatcgc
     3721 ggaagtactg gggcgggagg tgccgttcgc cgaggcaggc cgtcaggagg cccaccgccg
     3781 gatggccgcc ttcatgggcg gcgaagccgc tgacgcggtg ctcgacctga tgggcggcga
     3841 cgtcaacgac gaactgctcg ccgtccgcga cacggtcgcc cggatcaccg gcgcacgggc
     3901 caggacgttt cggcagtggg ccgcggagaa cgccgccgac ttccgctgag ccgcccggcc
     3961 cgggcccggc acggcccgcg cggcggccgg ccgtcccggc cccggagggc ggcccgcccc
     4021 cgcggacgac ggcgccgccc gggcacgcgg cgggggcggg gcgcaccagg cgccccgccc
     4081 ctgccgaccg gcgtacgccg ctacctcacc ggggtgaagt cccgcgcccc gatgaactcg
     4141 ggccggcgga tcggagccgc gaagggctcc tgggcggtgt tctcgacact gttgaacacg
     4201 atgaagacgt tgctgcgcgc gtacggggtg atgttgtccc ccgagccgtg catcgcgttg
     4261 cagtcgaacc aggtcgccga acccgccttg cccgtgaaga gcctgatgcc gtgccggtcg
     4321 gccatcttcg tcagcgcctc gtcggacggg gtgccggcgt cctgcatctg gagggacttc
     4381 ttgtagttgt ccttcggcgt ctcgcccgca cagcccagga acgtcttgtg cgaaccgggc
     4441 atgatcatca gcccgccgtt ggtgtcgaag ttctccgtca gcgcgatgga gaccgacacg
     4501 gtccgcatgt tcggcagccc gtcctcggcg tgccacgtct cgaagtccga gtgccagtag
     4561 aaaccggagg ccccgaagcc gggcttcacg ttgatccggg actggtggac gtagacgtcg
     4621 gagcccagga tctggcgggc ccggcccacc acacgctcgt cgcggaccag ccgggcgaag
     4681 acctcgctga tccggtggac ctcgaagacg gaccgtacgc tctgcgactt cggctcgatg
     4741 atcgaacgct cgtcggcccg gaccgccgga tcggcgatca gccggtccag ttcggcgacg
     4801 taccccgtca cctcgtccgg agtgatgagc tggtcgatgg cgaggaagcc gtcgtgctcg
     4861 aaggactgga ggtccttcgc ggcgatgggc ccgggcgcgc ccggcgcgga ccagatgacc
     4921 gggtcctggc ggggagtggt catctcggcg gcgccgcgcg tggggtagag gtcggttcgc
     4981 gcttcggcgg tggtcatggt tcagccctcc tcggtcagca gcgggtagac accgttctcg
     5041 tcgtggtcct cccgcccggt gaccggaggg ttgaagacgc agacgcaacg gaagtcggtc
     5101 ttcggccgca gcgtgtggtg ctcgtgcccg tccagcaggt acatcgtgcc cggcgagatc
     5161 cagtgcttct caccggtctc gtcgttggtg agttcggcct caccctcggt gcacagcacg
     5221 gcctcgatgt ggttggcgta ccacatcgac gtctccgtgc cggcgtacag cacggtctcg
     5281 tgcagggaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccacgtaccg
     5341 gaggccgact tgatgtgccg gtcggtgttc tcgatgtcac tgaacgaacg gacgatcacg
     5401 gtgtgatggt gcctttctgc tcggtactgc tcattcggtg cccgtgccgg gagccttccc
     5461 cggccgggcg gtctcggtgc cccggcgcgc cgcactccgt gccaccggcc gggggcggcg
     5521 ggccggcggc gaggggcggc ggcccccgcc gtgcggcggt gtgcgccgtc cggcggtgcg
     5581 gtgcgcgccg tgagggcggg tgtcaggcgg tctcgcggac ggaccgggcc agcgtgcgca
     5641 gaccctcgtc cagctcctcg ggggagatgg tcagcggcgg cagcagcttc acgacctcgc
     5701 tctgcgggcc cgaggtctcc agcagcagac cgagctcgaa ggcgcgggcg cagacggcgg
     5761 aggcccgcgc ggggtcggtg aactccaggc cccagaccag accgcggccc cggaactgcg
     5821 cctcggcctc ctcgccgcag atcgccagca gggtctgctc cacctgctcg ccgcgggcca
     5881 gggtctgctt ctccatctgg ccgtccgccc agtaggcgtc gagggcggcg gcggcggtca
     5941 cgaaggccgg gttgttgccg cggaaggtgc cgttgtgctc gcccggctcc cagatgtcca
     6001 gctcgccctt gaagaggcag agagacatcg gcaggccgta gccgctgatg gacttcgaca
     6061 gcgtgacgat gtccgggacg atgccggcct cctcgaagga gaagaacccg cccgtacggc
     6121 cgcagcccat ctggatgtcg tcgacgatca gcagcatgtc ctggcggtgg cacaggtcct
     6181 ggagcgcgcg gagccactcg gcgcgggcca cgttgatgcc gccctcgccc tgcacggtct
     6241 ccacgatcac cgcggccggc ttgttcagcc cggaaccctg gtcctccagg agccgctcga
     6301 accacaggaa gtccgggacc gtgccgtcga agtagttgtc gaagggcatc ggggtgccgt
     6361 gcaccagcgg gatgcccgca ccggcccgct tgaacgcgtt gccggtcacc gcgagcgacc
     6421 ccagcgacat gccgtggaag gcgttggtga aggagacgac ggactcacgg cccttgacct
     6481 tgcgggccag cttcagcgcc gactccacgg cgttggtgcc ggtcgggccg gggaacatca
     6541 ccttgtacgg caggtcgcgc ggacgcagga tcacgttctc gaacgtctcc aggaacgccc
     6601 gcttggccgt cgtggccatg tccaggccgt gggtgatgcc gtcacgctcg atgtagtcga
     6661 gcaacgcgcg tttcagcacc gggttgttgt gcccgtagtt gagtgatccg gcaccggcga
     6721 agaagtcgag gtacgagtgg ccgtcctcgt ccgtgagccg ggcgccctgc gcacggtcga
     6781 agaccgcggg ccagccgcgg cagtagctcc gtacctccga ctccaggctc tcgaagacac
     6841 tcagggcggg cggggtgatg gtcacagcgg atctcctgct cgacgagggg tgtgacgggt
     6901 ggcgggtgag gcgggcgccg ggcgctccgg acgaggcgta cgtccgccgg aaggggcggt
     6961 cacggacacg tcgtacacgg cccggtaggc cggagggggt gtacttccgg gcggccggga
     7021 cctacgcgaa cggtccgatg cggtacagga cttccggcag gtgcgtcccc tcggggaaca
     7081 gcccgccgtc gaagaggacc tcacgctcca gcggcacgtc gtggcgctgc gcgtaggagg
     7141 tgaacagccg gtcggaggcc gtgttgtccg gggtcacggt cgtctcgacc gcgtccaggc
     7201 cccgctcggc ggcgacacgg gaggtcagcg cgtccagcag ctcggcggcc agccccctcc
     7261 cgcggtgcgc gtggtccacg gccacctgcc agacgacaag ggtcccggga cggtcgggcc
     7321 ggaagtagcc cgtcacgaag gcgacgggct cgcccttctc gtcgcgggcg accacggagg
     7381 tcgccgcgaa gtcacggcac cacagcaggt agctgtacga ggagttgagg tccaggacct
     7441 cggaatcgcg ggcgatacgc cagatcgcgg ctccgtcctc cactcgcggg gtgtcgatcg
     7501 tcaggaattc ggttcgggca cgggcaatat ctgctggtgc ggcggtcatg ggaattgaat
     7561 ttacccagca aaaatcgaaa ttgcatcggc gcaaagggtt gggcggaagc ggctcctgtg
     7621 ttatcacgcg ggcgtgagac gagccgattc gcgtgaagat ttcccgcgat ttggcatgga
     7681 tttttccgac ataacgccca gctgtgtgga gttggtcaca gggttgtaac tctcctgaca
     7741 cgcgctcgga tcgagtgatt cgagcgcccc cgaaagagcg gagtttaggt tcgggaatcg
     7801 ggggcaaaag aatgcgggga gctgcttgga aaagcagctc cccgcatttg ccgataatgt
     7861 gtgcgcagag gtccggagag ggcgcccgga gacctccgtg cccttcgggt cagccgcccg
     7921 gccaggtttc ggagacggcc ttgcgggcag catccagatc ggccggacgg ccggtgtccg
     7981 ccagtgccga gccgagggcg gacagcgagg agagcacggc cccccgcgtc gcgtccacgc
     8041 cgtagtggtt gacgcggatc atctccgagg agagcgcccc gccgcccgcg atcagcggca
     8101 gcgaggggtc ggccgcgagc gcccgcgcca ccagggcgga ggcgtcgacc ccggccggcg
     8161 tacgcagcgt cgtggccacc ggcgcggcgt cccgggcgtc gtggacgtac ggcccgagac
     8221 cgcagcccag cgccagggcg cccgcccgcg tggcggcggc ggcagcggcg tgacgggccg
     8281 tcagcgtgtc caggccctcc gcctcgatcc gctccacaca cgcctccagt gccagcatct
     8341 ccagctgggc cggggcgtgc gggagcgcgg tgcggcccgg gtcgatccag cgggccttcc
     8401 agtccagcag ggagaggtag gaacggcggg gcgcccgcgg gttggactcc atccgctccc
     8461 acgcccgcgc gctcaccgac accgccgaca ccccggccgg cccgcccatc gccttctgcg
     8521 cgccgatcac gcacaggtcc acgccccacg cgtccggcag caggggctcg gcccccaccg
     8581 acgcgacggc gtccagcatg aacagcgcgc cgtgggcgcg gaccacctcg ccgatctccg
     8641 cgaccgggtt ggtgttgccg gtcgccgcct cggcgtgcac cagtgacacg aaatcgatct
     8701 ccgggtgtgc ggccagcgcc tcctccacct gcgcggcggt gaccgcggtg tggaagggca
     8761 ccgcgagatc gacgacctcc gcgccgcagt cccgcagcca gttcccgaag gtctggccgt
     8821 aggggccggt cacgacgttc agggcggtcg aaccggggcg ggcgccgccc cggatgcagc
     8881 cctccagggg cagcagcgcc tcgccttggg tgatcacgac gtcctgctcg gtggcgagga
     8941 ggccggccac ccggcgctcg atcgcagcga accgtgcggc ggtgagcggt gggaggtcga
     9001 ggaaggggtg cgtcacggga gtgctctctt cgaatctgtc gttccgctgt gtcgttccgc
     9061 gatcagcgct tgcgcgttcg agggtaccga gcacgccccg cgggcccgga cggtgtgcct
     9121 cgtacagtgc acacatgagc gatcacgagc cggcgcggac actgaggatc acggggcggg
     9181 tgctgctggg ccccgacgac gtcagggacg aggtctgggc cgtcggcggc cggatcaccc
     9241 acgaacggcc gcccggcgcc gacgacgcac tcaccctcac cggctgggcg ctgcccggcc
     9301 tcgtcgacgc ccactgccac gtcggcctcg acaagcacgg cccggtggac gccgcgaccg
     9361 ccgaagcgca ggcgctcacc gaccgcgatg ccggagccct gctcgtccgg gacgccggat
     9421 cgccctcgga cacccggtgg atcgacgacc gggaggacct ccccaagatc atcagagcgg
     9481 gccggcacat cgccaggacc cgccggtaca tccgcaacta cgcccatgag atcgaaccgg
     9541 gcgacctggt cgcccacgtc gcgcgggagg cgcggcgcgg cgacggctgg gtcaaactgg
     9601 tcggtgactg gatcgaccgc gacgccggcg acctgaccgc ctgctggccg cgcggcgagg
     9661 tcgaggcggc catcgccgag gcccaccggc tgggcgcccg ggtcaccgcg cactgcttcg
     9721 ccgaggactc gctgcgcgac ctggccgagg ccgggatcga ctgcgtcgag cacgcgaccg
     9781 gcctcaccga ggacaccgtc ccgctcttcg ccgaacgcgg tgtggcgatc gtcccgaccc
     9841 tggtcaacat cgcgaccttc cccggtctcg cggcagacgg cgaggccaag ttcccccgct
     9901 ggtccgccca catgcggcgg ctccacgcgc gccgctacga cacggtgcgc gcggcgtacg
     9961 acgcgggcat cgaggtgtac gtcggcaccg acgcgggcgg ttcgctggcc cacggcctgg
    10021 tggccgccga ggtcgccgaa ctggtcaagg ccggcatccc gcccctggaa gccctctccg
    10081 cgaccacctg gggcgcccgg cgctggctgg gacggccggt cctggaggag ggggcgccgg
    10141 ccgacctggt cctgtacgac gaggaccccc gggccgacgt acgcgtcctg gccgggcccc
    10201 ggcgggtggt gctgaacgga cgggtgatcg gctgaccggg gcggggcgcc ggtggaaacg
    10261 ccggtggtgt tgaccgacag tgacttccgg ccgccccgct cgttgaatct cttctgtgtg
    10321 cgggcgcgcc actcggccgc aggacggccg ccgttcggtg aaacgggtgg cttcagcgaa
    10381 ctcccgtgcc gaaagattc
//
