LOCUS       NZ_CP023702            10930 bp    DNA     linear   CON 23-APR-2024
DEFINITION  Streptomyces nitrosporeus strain ATCC 12769 chromosome, complete
            genome.
ACCESSION   NZ_CP023702
VERSION     NZ_CP023702.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nitrosporeus
  ORGANISM  Streptomyces nitrosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7581562)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008704555.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 03:20:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,704
            CDSs (total)                      :: 6,606
            Genes (coding)                    :: 6,461
            CDSs (with protein)               :: 6,461
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 66 of 146
            Pseudo Genes (incomplete)         :: 106 of 146
            Pseudo Genes (internal stop)      :: 16 of 146
            Pseudo Genes (multiple problems)  :: 38 of 146
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:19:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3982300
            Orig. end                         :: 3993230
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10930
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="17"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10930
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10930
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5930](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5930)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            1219..2730
                     /locus_tag="CP967_RS17735"
                     /old_locus_tag="CP967_17810"
     CDS             1219..2730
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (92-470). E-value: 4e-21.
                     Score: 67.7. Matches aSDomain:
                     nrpspksdomains_CP967_RS17735_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019992528.1"
                     /locus_tag="CP967_RS17735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17810"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_150488909.1"
                     /sec_met_domain="PF00561 (E-value: 9.6e-24, bitscore: 83.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTYARTRTALLLAAVTAASALTAVAFAPAAGAAGAGGTPALVWG
                     PCEPAGGPAGRECARLPVPLDYSDPSGPQVTLAVTRLRAQGPAPRRGTLLAVPGGPGGS
                     GVQRVTERGEALRSETEGRYDIVGFDPRGVGGSTKGSCSLAEEDRYMLTLRSWPAPDGG
                     IDENATRSRRVAAACARNGGPVVRGFTSENQARDMDRLRRALGERKVSVWATSYGAYTT
                     ALYAQHFPRRTDRLVLDSSGDPDPDRLARGWLANMARGAEDRFPDFAAWAADPPAGHRG
                     LRLAERPEDVRPLVTDLAARLDRAPRRAVNSDLPLNGNALRQALQSALYGDGAFAGFAA
                     LVKEAADPDGSPVLPPELAGPLPDTDAAVTIAVICNDVRWPASVEGYRSAVAADRAAHP
                     LTAGMPANITPCAFWKDAPTGRATRITGEGPSNILMIQSLRDPATPYTGALKMRAALGD
                     KARMVTTGRGGHGVYVPGRGACSDRAVAAFLNDGQRPAHDTHCAD"
     aSDomain        1495..2628
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP967_RS17735_Abhydrolase_1.1"
                     /evalue="4.00E-21"
                     /label="CP967_RS17735_Abhydrolase_1.1"
                     /locus_tag="CP967_RS17735"
                     /protein_end="470"
                     /protein_start="92"
                     /score="67.7"
                     /tool="antismash"
                     /translation="TLLAVPGGPGGSGVQRVTERGEALRSETEGRYDIVGFDPRGVGGS
                     TKGSCSLAEEDRYMLTLRSWPAPDGGIDENATRSRRVAAACARNGGPVVRGFTSENQAR
                     DMDRLRRALGERKVSVWATSYGAYTTALYAQHFPRRTDRLVLDSSGDPDPDRLARGWLA
                     NMARGAEDRFPDFAAWAADPPAGHRGLRLAERPEDVRPLVTDLAARLDRAPRRAVNSDL
                     PLNGNALRQALQSALYGDGAFAGFAALVKEAADPDGSPVLPPELAGPLPDTDAAVTIAV
                     ICNDVRWPASVEGYRSAVAADRAAHPLTAGMPANITPCAFWKDAPTGRATRITGEGPSN
                     ILMIQSLRDPATPYTGALKMRAALGDKARMVTTGRGGH"
     gene            complement(3046..3954)
                     /locus_tag="CP967_RS17740"
                     /old_locus_tag="CP967_17815"
     CDS             complement(3046..3954)
                     /NRPS_PKS="Domain: PKS_ER (20-300). E-value: 1.5e-39.
                     Score: 127.9. Matches aSDomain:
                     nrpspksdomains_CP967_RS17740_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155344.1"
                     /locus_tag="CP967_RS17740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17815"
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_150488910.1"
                     /sec_met_domain="ADH_N (E-value: 3.6e-05, bitscore: 22.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 3.8e-19, bitscore:
                     67.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLAIQFDRFGGPEVLRPVELPAPVPADGEVLLTVEAAGVNFADTH
                     QVDGSYLAADALPFVPGSEVVGRTPEGRRVLARVSRGYAELVAAKESSLVDLPEELGAA
                     EALALMMQGLTAWHLLRTAARVRPGETVVVHAAAGGVGSLAVQLAKEFGAGRVLAQAST
                     PDKERLALELGADGVAAYPLERGADVVLDAVGGELFDRALESLAPFGRLVTYGNAARNG
                     FTPVDPARLGRLSASVTGFWLRAALAAPDAVAGPLKELFGLVASGRLKPVTGPSYPLAD
                     ARRAHEDLLARRTVGKVVLRP"
     aSDomain        complement(3055..3894)
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP967_RS17740_PKS_ER.1"
                     /evalue="1.50E-39"
                     /label="CP967_RS17740_PKS_ER.1"
                     /locus_tag="CP967_RS17740"
                     /protein_end="300"
                     /protein_start="20"
                     /score="127.9"
                     /tool="antismash"
                     /translation="PAPVPADGEVLLTVEAAGVNFADTHQVDGSYLAADALPFVPGSEV
                     VGRTPEGRRVLARVSRGYAELVAAKESSLVDLPEELGAAEALALMMQGLTAWHLLRTAA
                     RVRPGETVVVHAAAGGVGSLAVQLAKEFGAGRVLAQASTPDKERLALELGADGVAAYPL
                     ERGADVVLDAVGGELFDRALESLAPFGRLVTYGNAARNGFTPVDPARLGRLSASVTGFW
                     LRAALAAPDAVAGPLKELFGLVASGRLKPVTGPSYPLADARRAHEDLLARRTVGKVVL"
     CDS_motif       complement(3508..3570)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CP967_RS17740_0001"
                     /evalue="6.90E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="CP967_RS17740"
                     /protein_end="149"
                     /protein_start="128"
                     /score="21.8"
                     /tool="antismash"
                     /translation="GETVVVHAAAGGVGSLAVQLA"
     misc_feature    4024..4042
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 21.06"
                     /tool="antismash"
     misc_feature    4025..4038
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.66"
                     /tool="antismash"
     misc_feature    complement(4030..4045)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    complement(4031..4040)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.17"
                     /tool="antismash"
     gene            4193..4954
                     /locus_tag="CP967_RS17745"
                     /old_locus_tag="CP967_17820"
     CDS             4193..4954
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-170). E-value: 6e-17. Score:
                     54.1. Matches aSDomain:
                     nrpspksdomains_CP967_RS17745_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047022.1"
                     /locus_tag="CP967_RS17745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17820"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_150488911.1"
                     /sec_met_domain="adh_short (E-value: 2e-35, bitscore:
                     121.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.1e-30, bitscore:
                     106.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLLSGKVVLITGASSGIGAAAARVFSQEGARVVLAARREDRLT
                     ELVDELKAAGGEAAHVVCDVTVAADAVRAVDFTVSTYGRLDGAFNNAGVGGDRTPLHLM
                     GDDVYDAVMDTNVRGVWNCIRPEIAAMLAHGGGAIVNNSSVGGLVAIPAASAYIAAKHA
                     VVGLTRAAADEYAGQGIRVNAVAPGTTRSEITADWFGRNPGLEEMVNSRTPQGRTAEPE
                     EIASAAAWLLSDRCPFLTGTVLPVDGGFINQ"
     CDS_motif       4217..4312
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CP967_RS17745_0001"
                     /evalue="1.20E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="CP967_RS17745"
                     /protein_end="40"
                     /protein_start="8"
                     /score="14.8"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAARVFSQEGARVVLAARR"
     aSDomain        4217..4702
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP967_RS17745_PKS_KR.1"
                     /evalue="6.00E-17"
                     /label="CP967_RS17745_PKS_KR.1"
                     /locus_tag="CP967_RS17745"
                     /protein_end="170"
                     /protein_start="8"
                     /score="54.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAARVFSQEGARVVLAARREDRLTELVDELKA
                     AGGEAAHVVCDVTVAADAVRAVDFTVSTYGRLDGAFNNAGVGGDRTPLHLMGDDVYDAV
                     MDTNVRGVWNCIRPEIAAMLAHGGGAIVNNSSVGGLVAIPAASAYIAAKHAVVGLTRA"
     gene            complement(5001..5930)
                     /locus_tag="CP967_RS17750"
                     /old_locus_tag="CP967_17825"
     CDS             complement(5001..5930)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="CP967_RS17750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17825"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_150488912.1"
                     /sec_met_domain="AfsA (E-value: 8.1e-16, bitscore: 56.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGGTVPEVPVFPHTLPAPAWGEQAVPRTYVHLSRDESVFVTGWR
                     RLKDGEFSLTARWPAAPRGLRYDPRVLTQTIRQSGLVVAHAAYGVPLSHQTLLHYFDFT
                     VNGDLRVHPAESASLGVEVTVSAPKKAGKAVSSLGMDVRVFQGDTMVARADSEFGWVSP
                     GAYRRLRGESLTAAWDDWPLPDPVEPRTVGRDRAGDVVLAPGGRPRRWWLRHDTSNHLL
                     YDHPVDHVPGLVLMEAADQAARAAVHPMPFEAGVVAASFERYVEFGRPCLIEAEVVPAA
                     GSDRVTVLVTGVQDEAVVFRGRLTGVCG"
     misc_feature    5974..5990
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.38"
                     /tool="antismash"
     misc_feature    5977..5986
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(5977..5990)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.77"
                     /tool="antismash"
     misc_feature    complement(5977..5991)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.06"
                     /tool="antismash"
     misc_feature    5978..5991
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.17"
                     /tool="antismash"
     misc_feature    complement(5979..5992)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.68"
                     /tool="antismash"
     gene            6021..6665
                     /locus_tag="CP967_RS17755"
                     /old_locus_tag="CP967_17830"
     CDS             6021..6665
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047020.1"
                     /locus_tag="CP967_RS17755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17830"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_150488913.1"
                     /transl_table=11
                     /translation="MARQQRAIRTRRIFLEAAAEVFDEHGYDAATIAAILDRAGLTRGA
                     LYFHFTSKEELARGVLQEAVTSDGVSPQIFKLQEWIDIALLLAYRLPREPLLSASIRLS
                     VDPRARGLFGTRWPDWITLGTEILTEAKERGELLPHVDPGATSRLFVGAWTGVQLITES
                     MPEPPDLVGEISALLELILPNNAVPGVLARLDTSPHRPERILEENRTAAAS"
     gene            6855..7634
                     /locus_tag="CP967_RS17760"
                     /old_locus_tag="CP967_17835"
     CDS             6855..7634
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967643.1"
                     /locus_tag="CP967_RS17760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17835"
                     /product="peptidoglycan recognition protein"
                     /protein_id="WP_150488914.1"
                     /transl_table=11
                     /translation="MWLRRSVLAAAFVPLAFLVFRDEPVPLVVPDEATARPETPAAPRP
                     AIVSRAQWHADESLVKEDATYTGAVNAVFVHHTGHPNDYDCADVPRMLRAMEEDHVRSE
                     GWDDIGYNFVVDRCGTVYEGRAGGVARAVRGAHTTGFNSHSVGIAAVGNFAPGVPVPEA
                     LIEGIAKVGAWKLRRGVDPEGTVRMVSTNDDSLFHRGQEVYLHAVSGHRDTFETNCPGD
                     ALYAQLPAIRLEMVRLRSSGGASARDGGSSPAPLPGG"
     misc_feature    complement(7654..7669)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            7739..8524
                     /locus_tag="CP967_RS17765"
                     /old_locus_tag="CP967_17840"
     CDS             7739..8524
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047018.1"
                     /locus_tag="CP967_RS17765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17840"
                     /product="transglycosylase family protein"
                     /protein_id="WP_150488915.1"
                     /transl_table=11
                     /translation="MPASPLAKRPVEAVFALLLVLLGALGLAGRSVAADRTAPAQATAV
                     QGAPAQAVPARTALGPHVTAGTDWDRIAECESGGRWDINTGNGYHGGLQFAPSTWQAYG
                     GTQYAPRADLATRAEQIAVGERVARSQGLSAWPTCGRLGANGAPDSSAAAPAPAPEQPS
                     ATDRRSSPGNQGSSGGSVVSGSTSDDQGDRSSRSTASGGTDDLVGAETYVVEPGDCLSV
                     IADRADVPGGTDALYALNRALLDEGPDLIYPGQRLRLTA"
     misc_feature    7770..7785
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.5"
                     /tool="antismash"
     gene            complement(8500..8751)
                     /locus_tag="CP967_RS17770"
                     /old_locus_tag="CP967_17845"
     CDS             complement(8500..8751)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155351.1"
                     /locus_tag="CP967_RS17770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17845"
                     /product="hypothetical protein"
                     /protein_id="WP_150488916.1"
                     /transl_table=11
                     /translation="MAGTERHQYDGQAAYVCPVCEHPVETVMGRHKTLGVFVPDRGPGP
                     CRNPECAVHVAEDEERTGEERAGGRAAREHGQAVRRRR"
     misc_feature    8787..8801
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.41"
                     /tool="antismash"
     gene            8819..9583
                     /locus_tag="CP967_RS17775"
                     /old_locus_tag="CP967_17850"
     CDS             8819..9583
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436180.1"
                     /locus_tag="CP967_RS17775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP967_17850"
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_150488917.1"
                     /sec_met_domain="HAD_2 (E-value: 4.1e-11, bitscore: 41.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEVLSPAAGLRSVRAVVFDTDGVITDSAPVHAAAWKKAFDACLA
                     SHEGQRPFDADEDYRRYVDGKARQDGAVSFLASRGIDLPEGTPDDPPGTATVRAVTARK
                     DEEFVRSLRERPVDVWPGTVRLLRVLRERGVPCAAASASRHATELISGAGLLDLFAAVV
                     DGNEARRLDLPGKPDPALFTEAARRLGTPPDDTAVVEDALAGVEAGRRGGFGLVIGVDR
                     SRSAAGAAGLRRHGADIVVPDLGDLLAEEETR"
     misc_feature    complement(10067..10081)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
ORIGIN
        1 cagcaggtag ccgtacatcg tgcccgcgcc gagcgccgtc atccacagct tgcggttcgg
       61 tacccaggcc aggaagcagg cgctcagcac caccgcgcag ccgaacagca gcagcgtcac
      121 cggcgcgccc acccaggccg gctcgcccag ctgctcggcc cccgccttgt ggaacagcca
      181 cgagtgcgag aaacgcggct cggcccagta ggcggcggcc agggcgatca ggcagacggg
      241 caccgaggcg acgcgcaccg cccggcggcg tacgaggtcg aagtgctcgg gccgcagctt
      301 gaggcccagg acgtagaacg gcaggaactg caggatccgc tggagctgga ggtcggtgcc
      361 gatggtgctg ttcatggagc cgatgcaggc gatgaccagc gacagcgaca gcggccaccg
      421 gaccgtctgc cagaacggcg tggtgatgcg ccagaggaag agcgccggca ggaaccacag
      481 gagccagtac ggggcgagcg ggctcagctc gtagcccggg gtcttcgtga ggcgttcgaa
      541 gaccgtgtac gcgacctcga agaccgcgta gggcaccagc acactggtga ccagcttcca
      601 catccggtcc ggccgggcgt cgaaactccg ggagaagtac ccggagacca tgatgaagac
      661 cggcatgtgg aaggcgtaga cgaacatgta gcacgtctcg acgaggcgcc cgctgtcacg
      721 gagcggctcc caggagtgcc ccagcgcgac gaggaccatg gccaggtact tggcgttgtc
      781 gaagtacgga tcgcgggcgc ggcggggtgc ggcgggtgcg gcggctccgg tggcggcggc
      841 cccggcgggg cccgggggcc gtgaggtcag ggagggcatc cggccacgat agaagacggc
      901 ccggggttcc ggttccccgg taccgcctcc tcccccgggc ccgggtggcc cccgggcccg
      961 gccccaggtc agccgccccg cccaccccgg cgttccgctt ccccgccgcc gcggcaccgc
     1021 gcggcccccg tgccgtgccg caccgcaccg tcggtccggt ccggcccggt tcggtccgtg
     1081 acggcggcgg ccggcggccg gcgggcgctg cccgggggac gggcccgcct gccccagggg
     1141 cacgccgggg gaaggcccca ccgcggtcgg cgggactgcc tcatgggcag cgggtgaacc
     1201 gccaaccacc ctgtgtacat gacgacttac gccagaacac gcaccgcact gctgctcgcg
     1261 gcggtcaccg ccgcctccgc gctgaccgcc gtggccttcg ccccggccgc gggggccgcc
     1321 ggtgcgggcg gcacgcccgc gctcgtctgg gggccctgcg agccggccgg gggccccgcc
     1381 ggccgggaat gcgcccggct ccccgtaccg ctcgactaca gcgacccctc gggcccgcag
     1441 gtcaccctcg ccgtcacccg gctcagggcc caggggccgg caccccgcag gggcaccctc
     1501 ctggccgtcc ccggcggccc cggcggttcc ggggtgcagc gcgtcacgga gcggggggaa
     1561 gcgctccgga gcgagaccga gggcaggtac gacatcgtcg gcttcgaccc gcgcggtgtc
     1621 ggcggcagca ccaagggctc gtgctccctg gccgaggagg accggtacat gctgaccctg
     1681 cggtcctggc cggccccgga cggcggcatc gacgagaacg ccacccgctc ccgccgcgtc
     1741 gccgcagcct gcgcccgcaa cggcggcccg gtggtacgcg gcttcaccag cgagaaccag
     1801 gcacgtgaca tggaccggct gcgccgcgcc ctgggcgagc ggaaggtctc cgtgtgggcc
     1861 acctcgtacg gcgcctacac gaccgccctc tacgcccagc acttcccgcg ccgtaccgac
     1921 cggctggtgc tggacagcag cggggacccc gacccggacc ggctggcacg cggctggctg
     1981 gccaacatgg cccgcggcgc cgaggaccgc ttcccggact tcgcggcctg ggccgccgac
     2041 ccgccggccg gacaccgcgg cctgcggctc gccgaacgcc ccgaggacgt ccgcccgctg
     2101 gtcacggacc tcgcggcgcg tctggaccgg gcaccccgcc gggccgtgaa ctcggacctt
     2161 ccgctgaacg gcaacgcgct gcgccaggcc ctgcagagcg ccctctacgg agacggcgcc
     2221 ttcgccggct tcgccgcact ggtcaaggag gccgccgacc ccgacggcag cccggtcctg
     2281 ccgcccgagc tcgccgggcc cctgcccgac acggacgccg ccgtcacgat cgcggtgatc
     2341 tgcaacgacg tacgctggcc cgcctcggtc gaggggtacc ggagcgcggt ggccgccgac
     2401 cgcgccgccc acccgctcac cgcggggatg cccgccaaca tcacgccctg cgccttctgg
     2461 aaggacgccc ccaccggcag ggccacccgg atcaccggcg aaggaccgtc gaacatcctg
     2521 atgatccaga gcctccggga ccccgcgacc ccctacacgg gggcgctgaa gatgcgcgcg
     2581 gccctcggtg acaaggcgcg gatggtcacc acgggccgcg gcgggcacgg ggtgtacgta
     2641 ccgggacgcg gagcctgcag cgaccgggcc gtcgccgcct tcctcaacga cgggcagcgc
     2701 cccgcccacg acacgcactg cgcggactga cccggcaccg ctcaggccgt ccccgggggc
     2761 gggccgcagc ggtcctcccc ggggacggtg gagctcaagg gccccgaggc cccgtggccc
     2821 cgcgccccga ggccccgggg gcctcgctca ggccgccggc cccggcccgg aacccgcttg
     2881 agcccccggg aacccgctca ggccgccggg gccggtccgg tgcccgctca aggtcgccgg
     2941 ggccgctctg gcacccgctc agaccaccgg gccccgctcc ggcaccagct cagaccgccc
     3001 ccgggccccg ctccggcacc agctcaggcc gtccccggga cccgctcagg gcctcagcac
     3061 caccttgccg accgtccggc gcgccaacag gtcctcatgg gcccgccggg cgtcggcgag
     3121 cgggtaggaa gggccggtca ccggcttcag acggcccgag gcgaccagcc cgaagagctc
     3181 cttcagcggc ccggcgaccg cgtcgggcgc cgccagcgcg gcacgcagcc agaagcccgt
     3241 gaccgaggcg ctgagccggc cgagccgggc cggatcgacg ggggtgaagc cgttccgggc
     3301 ggcgttgccg tacgtcacga gccggccgaa gggcgccagg gactccagcg cccggtcgaa
     3361 gagctccccg cccaccgcgt ccagcaccac gtccgccccg cgctccagcg gataggcggc
     3421 caccccgtcc gcccccagct ccagggccag ccgctccttg tccggggtgg acgcctgggc
     3481 cagcacccgg cccgccccga actccttcgc cagctgcacg gccaggcttc ccaccccgcc
     3541 cgccgccgcg tgcacgacga cggtctcccc gggccgcacc cgggccgccg tacgcagcag
     3601 gtgccaggcc gtcagcccct gcatcatcag ggccagcgcc tcggcggcac cgagctcctc
     3661 cggaaggtcc acgagcgagg actccttggc ggccaccagc tccgcgtacc cccgcgacac
     3721 ccgggccagc acccgacggc cctccggcgt acggcccacg acctcactgc ccgggacgaa
     3781 cggcaacgcg tccgcggcca gataggaacc gtccacctga tgggtgtccg cgaaattcac
     3841 cccggcagcc tcgacggtga gcagtacctc gccgtccgcc ggtacgggcg cggggagttc
     3901 gacgggccgc aggacttcgg gaccgccgaa acggtcgaac tggatagcga gcatgcggca
     3961 cggtaccgcg cacggcgccc ctgtggccgg acggcccgtt cgccctcggg ggaaccccgt
     4021 ccctggggta taaaaaaacc gttcggacgg tttgttgagg ggcggccgcc ctgtgtgcgc
     4081 cgggccgggg aaggctccgg ggccggagag cgcgcctgtc ccggaccgcc cggtacggct
     4141 atggcgcgga ccggctcgtt ccggtgggat ccaccactgc cagactgccg ccatgagtga
     4201 tctcctcagc ggcaaagtcg tcctcatcac cggggcgagc agtggcatcg gcgcggcagc
     4261 ggcacgtgtg ttctcccagg agggcgccag ggtggtcctg gcggcccgga gggaggaccg
     4321 gctgaccgag ctggtcgacg agttgaaggc agcgggcggc gaggccgccc acgtcgtctg
     4381 cgacgtcacc gtcgcggcgg acgcggtccg ggccgtggac ttcacggtca gcacctacgg
     4441 caggctggac ggggcgttca acaacgccgg tgtcggcggg gaccggaccc ccctgcacct
     4501 catgggggac gacgtctacg acgcggtcat ggacaccaac gtacggggcg tctggaactg
     4561 catccgcccc gagatagcgg cgatgctcgc ccacggcggc ggcgcgatcg tgaacaacag
     4621 cagcgtgggc ggcctggtgg cgatacccgc ggcctccgcc tacatagcgg ccaagcacgc
     4681 ggtggtcggc ctcacccgcg ccgccgccga cgaatacgcc ggacagggca tacgcgtcaa
     4741 cgccgtcgcc ccgggaacca cccgcagcga gatcaccgcc gactggttcg ggcggaaccc
     4801 cggcctggag gagatggtca acagccgcac tccgcaaggg cgtacggccg aacccgagga
     4861 gatagcctcc gccgccgcat ggctgctcag cgaccgctgc cccttcctca ccggcacggt
     4921 cctcccggtg gacggcggct tcatcaacca gtgaagaccg gcggctgacc ggggtgtgcg
     4981 gccgggcggg gccgccccgg tcagccgcac accccggtca gccgcccccg gaagaccacc
     5041 gcctcgtcct gcaccccggt gaccaggacc gtgacccggt ccgaaccggc ggcgggcacc
     5101 acctcggcct cgatcaggca gggacggccg aactcgacgt accgctcgaa cgaggcggcc
     5161 accacgcccg cctcgaaggg catcggatgg acggcggccc gcgcggcctg gtcggcggcc
     5221 tccatcagta ccagccccgg gacatggtcg accgggtggt cgtacagcag atggttggac
     5281 gtgtcgtggc ggagccacca ccggcgcggc cggccgccgg gggccaggac cacgtccccg
     5341 gcccggtccc ttcccacggt gcggggctcc acggggtccg ggagcggcca gtcgtcccac
     5401 gcggccgtga ggctctcccc gcgcagccgc cggtacgcgc ccggcgagac ccagccgaac
     5461 tcggagtcgg cccgcgcgac catggtgtcc ccctggaaca cgcggacgtc catgcccagt
     5521 gaactgaccg ccttgccggc cttcttcggc gcggacacgg tgacctcgac gccgagggag
     5581 gcggattcgg ccggatgtac ccgcagatcc ccgtttacgg tgaagtcgaa gtagtgcagg
     5641 agggtttggt gggacagtgg gaccccgtac gccgcgtgtg ccaccaccag gccgctctga
     5701 cggatggtct gggtgagcac ccgcgggtcg taccgaaggc cccgcggggc cgccggccac
     5761 cgggcggtga gcgagaactc gccatctttc aggcggcgcc atccggtgac gaagaccgac
     5821 tcgtcccggc tgaggtgaac atatgtccgc ggaaccgcct gttcgcccca ggcgggggcg
     5881 ggcagagtgt gcggaaaaac aggtacctcg ggcacggtcc ccccggacat ggcgcaaatt
     5941 ggcaagcctg gcgctgatat gataccggtt tgccggtatt ttttgaagaa gcgtcagatc
     6001 gaggtcaagg ggagcgcgag ttggcgaggc agcaacgggc gatccgtacg cgccgaatct
     6061 tcctggaagc cgcagccgag gtgttcgacg aacacggtta cgacgccgcc acgatcgccg
     6121 cgatcctcga ccgcgccggc ctcacgcggg gcgcgctgta cttccacttc acctcgaagg
     6181 aggagctggc ccgcggtgtc ctccaggagg ccgtgacctc ggacggcgtc tccccgcaga
     6241 tcttcaagct gcaggaatgg atcgacatag cgctcctcct cgcctaccgg ctgccccggg
     6301 agccgctgct cagcgcctcg atccggctgt ccgtcgaccc ccgggcacgc gggctcttcg
     6361 ggacccgctg gccggactgg atcacgctcg gcaccgagat cctcaccgag gccaaggagc
     6421 gcggtgagct gctgccgcac gtcgatcccg gtgcgacctc ccggctcttc gtcggggcct
     6481 ggaccggtgt ccagctgata acggagtcca tgcccgagcc gccggacctg gtcggcgaga
     6541 tctccgcact cctcgaactg atcctcccca acaacgccgt ccccggcgtg ctggcccggc
     6601 tcgacacctc cccgcaccgg cccgagcgca tcctggagga gaaccggacg gccgccgcgt
     6661 cctgaccgcc gcgtcctgcg ccgcagcgcg caggaccgcg ccgcgcaccg ggcccccgcg
     6721 caccgggcca ctgctccccg gctgccgcgc gcgggtccgc cgggagcgcg ggcgcacctg
     6781 gcggggcccg gcggcgtcac ccgaacgggc gggcggggcc tgcgaagccg ctggtcagcg
     6841 ggcaggctgg ccccatgtgg cttcgccggt cggtcctggc cgccgccttc gtaccgctgg
     6901 ccttcctggt gttccgggac gagccggtgc cgctcgtcgt gcccgacgag gccaccgcgc
     6961 gccccgagac cccggcggcc ccccggcccg cgatcgtcag ccgggcgcag tggcacgcgg
     7021 acgagagcct ggtcaaggag gacgcgacct acaccggggc ggtgaacgcg gtcttcgtgc
     7081 accacacggg gcaccccaac gactacgact gcgccgacgt cccccgcatg ctccgggcca
     7141 tggaggagga ccacgtcagg agcgagggct gggacgacat cggctacaac ttcgtggtcg
     7201 accgctgcgg cacggtctac gagggccggg ccggaggcgt cgcccgcgcc gtgcgcggcg
     7261 cccacacgac cgggttcaac tcccacagcg tgggcatcgc cgccgtgggc aacttcgccc
     7321 ccggggtgcc ggtgcccgag gcgctcatcg agggcatcgc gaaggtgggc gcctggaagc
     7381 tgcgccgggg tgtcgacccc gaggggaccg tacggatggt ctccacgaac gacgacagcc
     7441 tcttccaccg cgggcaggag gtgtacctcc acgccgtgtc gggtcaccgg gacaccttcg
     7501 agaccaactg ccccggtgac gccctctacg cccaactccc ggcgatccgc ctggagatgg
     7561 tgcgcctgcg ttcctccggc ggggcctcgg cgcgggacgg cggcagcagc cccgccccgc
     7621 tgccgggcgg gtgaaaagca gaagatccgc cgcgtcaaag ccctgtgaaa gccatatcgg
     7681 cggtttatct ggtcgagccc gagccagacc ttctggagca cagcggagga ataccgtcat
     7741 gccggcatca cccctcgcca agcggcccgt cgaagccgtg ttcgctcttc tgctcgtcct
     7801 tctgggcgcg ttgggcctgg ccggccgcag cgtggccgcc gaccgtaccg cccccgcgca
     7861 ggccaccgcc gtacaggggg ccccggcgca ggcggtcccc gcccgtaccg ccctcgggcc
     7921 ccacgtcacg gcgggtaccg actgggaccg catcgccgag tgcgagagcg gcggccggtg
     7981 ggacatcaac accggcaacg gctaccacgg cggactccag ttcgcgccct ccacctggca
     8041 ggcgtacggc ggcacccagt acgcgccgcg tgccgacctc gcgacccgcg cggaacagat
     8101 cgccgtcggt gaacgcgtcg cccgctcgca gggactgtcc gcctggccga cctgcggacg
     8161 cctcggagcg aacggcgcac cggactcctc cgccgccgcc cccgcccccg cccccgaaca
     8221 gccttccgcc accgaccgcc ggagcagccc cggaaaccag gggagctccg gcggttccgt
     8281 cgtttccgga agcacgtcgg acgaccaggg cgaccggtcg tcccggagca cggcgtccgg
     8341 cggcaccgat gacctcgtcg gtgccgagac ctacgtcgtc gagccgggcg actgcctgtc
     8401 cgtgatcgcc gaccgggccg acgtccccgg gggcaccgac gccctgtacg cactcaaccg
     8461 cgccctgctg gacgagggcc cggatctgat ctacccgggt cagcgcctcc gcctcaccgc
     8521 ctgaccgtgc tcccgggccg cccggccccc ggcccgctcc tcacccgtcc gctcctcgtc
     8581 ctccgccaca tggacggcgc actccgggtt ccggcagggg cctggacccc ggtcggggac
     8641 gaagacgccg agggtcttgt gccgccccat caccgtctcg acggggtgct cgcagacagg
     8701 acagacgtag gcggcctgcc cgtcgtactg atgacgctcg gttccggcca tgtattcacg
     8761 gtagcccggt accagggcga aacgtaatct ctccgactat tcacccggga gtccggtcat
     8821 ggcagaggta ctgtcgccgg ccgcgggtct gcgatccgtt cgcgccgtcg tcttcgacac
     8881 cgacggagtg atcactgatt cggccccggt gcacgcggcc gcctggaaga aggcgttcga
     8941 cgcctgcctc gcgtcccacg aaggccagcg gcccttcgac gcggacgagg actaccgccg
     9001 ctacgtcgac ggcaaggcac ggcaggacgg cgcggtgtcc ttcctcgcgt cgcgcgggat
     9061 cgacctcccc gaaggcaccc cggacgaccc gcccggcacg gcgacggtac gggccgtcac
     9121 cgcccgcaag gacgaggagt tcgtacggag cctgcgtgaa cgccccgtcg acgtctggcc
     9181 gggcacggtc cggctcctgc gcgtgctgcg cgagaggggc gtgccctgcg cggcggcttc
     9241 cgcgtcccgg cacgcgaccg aactgatctc aggggcgggg ctgctggacc tgttcgccgc
     9301 cgtcgtcgac ggcaacgagg cccgccggct ggacctgccc ggcaaaccgg acccggccct
     9361 gttcaccgag gcggcccggc gcctgggcac acccccggac gacaccgcgg tggtggagga
     9421 cgcgctggcc ggtgtcgaag cgggccggcg cggcggcttc ggcctggtga tcggggtgga
     9481 ccggtcccgg agcgccgccg gcgccgcggg gctgcgccgc cacggcgccg acatcgtggt
     9541 gcccgacctc ggggacctgc tggccgagga ggagacccga tgagcgcacc gtcggcccag
     9601 ccggcgccgc cgacggcggc ggagccgtgg acctgggagt acgagggcta cgacccggag
     9661 aaggagcggc tgcgcgaggc cctgtgcacg ctgggcaacg gctacttcgc cacccgcggc
     9721 gccgcccccg agacccacgc ggacacgtcc cactacccgg gcacctatgt ggcgggcgtc
     9781 tacaaccggc tcgtctcctc cgtccagggc ggccgggacg tccagaacga ggacatggtc
     9841 aacctgccga actggctgcc gctgcgctac cgcgtccggc ccacgggcgg cacccccgga
     9901 ccctggctgc accccgacca ctcctacctc gtggaacacc ggcagtggct cgacctgcgc
     9961 ggcggcaccc tgctgcggcg cagcgtctac gaggacggcg cgggccggcg gctcgtggtg
    10021 gaacaggaac gcctggtgca catgggcgac ccccatctcg ccgcgctgca cacggtgttc
    10081 accgccgagg gctggaccgg ggagctggag gtggtggccg gcatcgacgg cgacgtgacc
    10141 aacagcggcg tcgcccgcta ccgggacctg gcgaaccgcc acctgacggg ctgggaaacg
    10201 ggcgtggagc gcggttcgac cgtctggctg cgctgtcgca ccgtggagtc cgacatcggg
    10261 ctcgccctgg ccgcccgcac cgtggccacc ttcggtacgg cggagagcgg cggtgaccac
    10321 cacgcgcacc ccgagcagct gccccggggc gtccagcagc acctgctggt cccgatggga
    10381 ccgggctccc cggccgtcgt cgacaagacg gtcgccctgt acacctcgcg tgaccaggcc
    10441 atcggcagcc cccagcgggc cgcggtgggc gcggtggccc ggagcccgga gtacggggaa
    10501 ctgctcgcct cccaccaccg ggcctggggg gacctgtggc agcgggtcca ggtggaggtg
    10561 ccgggcgagc cggggcacat cctgcgcctg cacctcttcc acgtgctcca gaccctctcc
    10621 ccgcacaccg ccgacctcga cgccggggtg ccggccagag ggctgcacgg ggaggcatac
    10681 cggggccatg tgttctggga cgaactgttc gtcctgccct tcctcaacct ccatctcccg
    10741 gaggtgtcaa gggcgttgct ggactaccgg caccgccggc tgcccgccgc ctgccgggcg
    10801 gcccgcgcgg cgggcaggcg cggcgcgatg ttcccgtggc agagcgccgc cgaaggccgt
    10861 gaggagaccc agcaggtcca cctcaacccg cgctcgggcc gctggctgcc cgaccactcc
    10921 cacctccagc
//
