LOCUS       NZ_CP023697            11344 bp    DNA     linear   CON 12-OCT-2024
DEFINITION  Streptomyces prasinus strain ATCC 13879 chromosome, complete genome.
ACCESSION   NZ_CP023697
VERSION     NZ_CP023697.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces prasinus
  ORGANISM  Streptomyces prasinus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7647592)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008704445.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:58:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,683
            CDSs (total)                      :: 6,591
            Genes (coding)                    :: 6,375
            CDSs (with protein)               :: 6,375
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 81 of 216
            Pseudo Genes (incomplete)         :: 177 of 216
            Pseudo Genes (internal stop)      :: 19 of 216
            Pseudo Genes (multiple problems)  :: 56 of 216
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:55:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6258964
            Orig. end                         :: 6270308
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023697.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11344
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11344
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11344
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6344](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6344)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1276..2340)
                     /db_xref="GeneID:95538353"
                     /locus_tag="CP972_RS28100"
                     /old_locus_tag="CP972_28155"
     CDS             complement(1276..2340)
                     /codon_start=1
                     /db_xref="GeneID:95538353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034031.1"
                     /locus_tag="CP972_RS28100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28155"
                     /product="DUF4349 domain-containing protein"
                     /protein_id="WP_150475528.1"
                     /transl_table=11
                     /translation="MRTRRSVRPAHAVTGLLLAAALALAGCGAGSGSDSGASSSSDDKA
                     ALSQADARGDAQEGAKEGTARDGAAAGSADGSPATAPPADRNTATNHVIRTASLTVRVK
                     DAPAALAEARTATENAGGYVGDETTRQDAEGNERTRVVLRVPAEKYDAVLADLEGSGEL
                     VERRAKAEDVTDQVVDVESRIKTQRTSVARIRELMDRAVRLSDVVTLEGELSTRQADLE
                     SLLARQASLKDRTGLATVTLTLSETPVERSTEDDDPGFLDALAGGWDAFVTMLRWLAVA
                     IGAALPFAALAALLALLWLKAVRPGLSRRTRPAPSSPSVPASPSVPAARPASAPTTART
                     PDEAPKEAPGPETK"
     misc_feature    2455..2474
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.24"
                     /tool="antismash"
     misc_feature    complement(2457..2471)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.27"
                     /tool="antismash"
     misc_feature    2458..2472
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.07"
                     /tool="antismash"
     gene            2509..3945
                     /db_xref="GeneID:95538354"
                     /gene="hemG"
                     /locus_tag="CP972_RS28105"
                     /old_locus_tag="CP972_28160"
     CDS             2509..3945
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95538354"
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981981.1"
                     /locus_tag="CP972_RS28105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28160"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_055607687.1"
                     /sec_met_domain="DAO (E-value: 1.9e-11, bitscore: 42.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGTHTGAEQRVVVVGAGIAGLAAAHRLLGRGARVTVLEASERVG
                     GKLLPGEIAGARVDLGAESMLARRPEAVDLAREVGLTDRLRPPATATASLWTRGALRPM
                     PKGHVMGVPGTAAALAGVLSDEGLARIGQDAELPRTEVGDDVAVGAYVAARLGREVVDR
                     LVEPLLGGVYAGDAYRISMRSAVPQLFRAARTHTSLTEGVREIQAAATGQRTGPVFMGI
                     EGGVGTLPLAVAESVRSRGGEIRTGAPVTELRREPAGGWRVVTGERELHADAVVVAVPA
                     PAAARLLRAEAPGAAAELAGVEYASMALITLAYRRAETDLPEGSGFLVPPVDGRTIKAS
                     TFASRKWDWIDEENPDLTLLRTSVGRHGETEILGRDDAGLVEVSRHDLLAATGLDATPV
                     ATRVTRWQDGLPQYPVGHHARVARVREHLAALPGLAVCGAAYDGVGIPACIASAYDAAD
                     AVGQIHGEAPHDVRDVTATR"
     gene            3981..4712
                     /db_xref="GeneID:95538355"
                     /gene="hemQ"
                     /locus_tag="CP972_RS28110"
                     /old_locus_tag="CP972_28165"
     CDS             3981..4712
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /db_xref="GeneID:95538355"
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133767.1"
                     /locus_tag="CP972_RS28110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28165"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_055607686.1"
                     /transl_table=11
                     /translation="MTDDATTPAPDRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAESSDQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADEEPRAYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGSEARMHVR
                     EEIPFYTGRRKDIAELVAGLA"
     gene            complement(5001..6344)
                     /db_xref="GeneID:95538356"
                     /locus_tag="CP972_RS28115"
                     /old_locus_tag="CP972_28170"
     CDS             complement(5001..6344)
                     /codon_start=1
                     /db_xref="GeneID:95538356"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122140.1"
                     /locus_tag="CP972_RS28115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28170"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_055607685.1"
                     /sec_met_domain="DUF692 (E-value: 6e-96, bitscore: 319.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAGDIERMPGIDWVEVVAENVCPGHVPASLLR
                     LRERGVTVVPHGVALGLGGAERPDAGRLEALAERAEALRSPLVTEHIAFVRAGGPLSAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPGEEMSEGQFLY
                     DLADRTGVRLLVDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGVERDGVWHDSH
                     AHPVPRPVLDILTDLASRVSPPGVLLERDEDFPAPDELERELTGIREAVAKGAAARTTA
                     RAQGSGVRSRSVPVSGSARESGSRGPARQRVALAQAAVLSSLVAGTPVPEGFDRARMAV
                     QARALAAKRADVVAKVAPELPVLLGERYRAAFLAHAQRWPMTGGYRQDALDFVERLLLT
                     SGQRERRDEQLPLRELREWWLERSGPVPHSRRPAARLARATRRVLLRR"
     misc_feature    complement(6467..6476)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(6660..7085)
                     /db_xref="GeneID:95538357"
                     /locus_tag="CP972_RS28120"
                     /old_locus_tag="CP972_28175"
     CDS             complement(6660..7085)
                     /codon_start=1
                     /db_xref="GeneID:95538357"
                     /inference="COORDINATES: protein motif:HMM:NF040404.3"
                     /locus_tag="CP972_RS28120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28175"
                     /product="hypothetical protein"
                     /protein_id="WP_055607684.1"
                     /transl_table=11
                     /translation="MTEFEFHVDPADPTIRAGLERWLLEDDRLRGSLRLSTASPRRSPA
                     HDEYLGDPLSVLALVLSTTLALPAFLDSVRRWFATRPPQAPPLTLTRGAVTVLIPQDMD
                     TVKVAELADALASAESPRPADDPVDDTSGGSSGPAST"
     gene            complement(7104..8333)
                     /db_xref="GeneID:95538358"
                     /locus_tag="CP972_RS28125"
                     /old_locus_tag="CP972_28180"
     CDS             complement(7104..8333)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95538358"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /locus_tag="CP972_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28180"
                     /product="TIR domain-containing protein"
                     /protein_id="WP_055607683.1"
                     /transl_table=11
                     /translation="MSQTAKPPFLFISYAEPDRSWAEWVAWELQEAGHRVRLDRWDWRA
                     GDNRQIELGHAVENGQVLALFSEAYFDPRHSTADHWAAVLAAGDRIIPVRIDETEPPKL
                     LRPLLALSLYGLDHESARRELLKVLHAPGRPRTRPPFPGATGDTARARHPSPPPPIRRP
                     SDWSTGACVLVGVHTYQELSDQPGVKGNLEDLRKLLTTSFGIPRDRCLVVDNPLDPRAV
                     MDAIERAGRATAFESGMLLVYYAGHGVPHPRTGQLLLSVADSRVSSPHTFLPFEQVREA
                     VATSPASQRLVIIDCCYSARGLDALAAAGPLLPPIEGTFLMASSGATEVSLAPRGRPRT
                     AFTDALLGILEQGLPGGPPLLDAESLFEGARRVCADRKWPGPHRQVRNDGHLIPLIPNR
                     WHPGPPPAAD"
     gene            8499..9296
                     /db_xref="GeneID:95538359"
                     /locus_tag="CP972_RS28130"
                     /old_locus_tag="CP972_28185"
     CDS             8499..9296
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95538359"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /locus_tag="CP972_RS28130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_28185"
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="WP_055607682.1"
                     /transl_table=11
                     /translation="MGSHKGQEGLREQYKFGDSTTAETRLVTVARVFEPSSRAFLQRHA
                     APGASLAVDLGCGPGHSTRLLHEVVRPDRTLGLDRSASFLATASASALPGMLFRRHDVT
                     VTPLPVEQPDILYARLLLSHLKEPVRMVRAWVAELTPGGSLLLDEVESVETSDETGRDY
                     QKVVQSMLLDNGQRLDVGRLLTAASPAGAGRVRQELVTVRPTSRQVGLMFALNLRVWRS
                     DPFLAARYSPDWLDGLQAELDRAAEGLRSLTVTWTMRQLALTR"
     misc_feature    8820..8822
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 accccgagca ggcggcccgt gcgcagttcg gcgagcatct tcaccgtcat gggggaggcg
       61 ttcgggtagt agcccgcgcg gctggtcgac tcgatggtga ccgtctcgaa ccgcagcccc
      121 gcgcggtggg cgtccttctc ccgcaggccg gtgcgcgcga tctccaggtc gcagaccttg
      181 ctgaccgcgg taccgaccac acccgggaag gtggcgtagt cgccgccgac gttcgagccg
      241 atgacctggc cgtgcttgtt ggcgtgcgtg ccgagcgcga tgtgccgctc ctggccggag
      301 accaggtcga gcacctccac gcagtcgccg ccggcccaga tgttctcgtg gccgcggacc
      361 cgcatcgacc ggtcggtgag caggccgccg tgttcgccca ggggcagccc cgccgcccgc
      421 gcgagagccg tttcgggccg caccccgatg ccgagcacca ccacgtccgc cgggtactcg
      481 gcgtccgcgg tggtcacggc ccgtaccctg ccgtcgtccc cggtgagcag tccggtgacc
      541 tcggcgtcgt tcaccatggt gatgcccagg ccctccatcg cctcgtgcac caggcgcccc
      601 atgtcggggt cgagggtcgc catgggctcc cggccgcggt tgacgacggt cacctcgtag
      661 ccgcgtcgga tcatcgcctc ggccatctcc acgccgatgt accccgcgcc gacgaccacc
      721 gcccggcggc cggccgtgcg ctccagcgtg tcgagcaggg cctggccgtc ctccagggtc
      781 tgcaccccgt gcacaccggg ggcgtccacg ccgggcagct ccgggcggac cggccgcgcg
      841 ccggtcgcga tgacgagctt gtcgtacgac gtccaggact cggtgccgga atcgaggtcc
      901 cgcgcgcgta cccgcccccg gtcggggtcg atctccgtga cctcggtgcg catccgcagg
      961 tcgatgcggc gcgcgcggtg ctcctcgggc gtgcgggcga tcagctcgtc gcggtcggtg
     1021 acgtcaccgg ccacccagta ggggatgccg caggccgaga aggacgtgaa gcgcccgcgc
     1081 tcgaacgcca cgatctccag ctcgtccggc cccttcagcc ggcgcgcctg cgacgccgcg
     1141 gacatccccg cggcgtcgcc gccgatcacc accaggcgtt ccggcccctt cgaggccgga
     1201 accgcgccgc cgcccgtacc cgtactcgtc cctgtgctcg tgctcgtggt catacggcca
     1261 cgctacgggg accggtcact tcgtctccgg ccccggggcc tccttcgggg cctcgtccgg
     1321 agtgcgggcc gtcgtgggcg cggacgcggg ccgggccgcg gggacggagg gggaggcggg
     1381 aacggacggg gacgaggggg ccggccgggt gcggcgggac agcccggggc gtacggcctt
     1441 cagccacagc agggcgagca gggccgccag agccgcgaac ggcagcgccg cgccgatcgc
     1501 caccgcgagc cagcgcagca tggtcacgaa cgcgtcccag ccgcccgcga gcgcgtccag
     1561 gaatccgggg tcgtcgtcct cggtgctccg ctccaccggc gtctcggaca gggtcagggt
     1621 gaccgtggcc aggccggtgc ggtccttcag ggacgcctgc cgggcgagca gggactccag
     1681 gtcggcctgg cgggtgctga gttcgccctc cagggtgacc acgtcactga gccggacggc
     1741 ccggtccatc agctcgcgga tcctggccac gctggtgcgc tgcgtcttga tccggctctc
     1801 gacgtcgacg acctggtcgg tgacgtcctc cgccttcgcg cggcgttcga cgagttcgcc
     1861 ggagccctcg aggtccgcga ggaccgcgtc gtacttctcg gcggggacgc gcagcaccac
     1921 gcgcgtgcgt tcgttgccct ccgcgtcctg acgggtggtc tcgtcgccga cgtagccgcc
     1981 cgcgttctcc gtggccgtgc gtgcctcggc cagcgccgcc ggcgcgtcct tcacccgcac
     2041 ggtcagcgag gcggtgcgga tgacgtggtt cgtggcggtg ttcctgtcgg cgggcggcgc
     2101 cgtcgccggc gagccgtccg cggagccggc ggcggccccg tcccgcgccg tcccctcctt
     2161 cgccccttcc tgagcgtccc cccgggcgtc ggcctgggag agggcggcct tgtcgtcgga
     2221 ggagctggag gcgccggagt ccgaaccgga gcccgcgccg catccggcca gggcgagcgc
     2281 ggcggccagc agcaggcccg tcacggcgtg ggcgggccgt acggatcgtc gtgtgcgcat
     2341 gtgggttccc cccgggttcg gtaccaccga ccgtttctct gacgctcctt cgacgccgga
     2401 gggcggtgga acgatgacgc ggaaaggtcc cgaagcggtc acggtaggga ctcggagggg
     2461 ccgtacggcc ctggtgggac cgccggcggc atctgagagg gtggagccat gagcggaaca
     2521 cacacgggtg cggagcagcg ggtcgtcgtc gtgggggccg gcatcgccgg gctggccgcc
     2581 gcgcaccggc tgctcgggcg cggggcgcgg gtgaccgtgc tggaggcctc ggagcgggtc
     2641 ggcggcaagc tgctgcccgg cgagatcgcg ggcgcgcgcg tggacctggg cgccgagtcg
     2701 atgctggccc gccgcccgga ggcggtggac ctcgcccgtg aggtgggcct caccgaccgg
     2761 ctccggcccc cggccaccgc gaccgcgtcg ctgtggaccc gcggcgccct gcgccccatg
     2821 cccaagggcc acgtcatggg tgtccccggc accgccgccg ccctggccgg ggtgctctcc
     2881 gacgagggcc tcgcccggat cggacaggac gccgaactgc cccgcaccga ggtcggcgac
     2941 gacgtcgccg tcggcgcgta cgtggcggcc cggctcggcc gcgaggtcgt cgaccggctg
     3001 gtcgaacccc tgctgggcgg ggtgtacgcg ggcgacgcgt accgcatctc catgcgctcc
     3061 gccgtccccc agctgttccg ggccgcgcgc acgcacacct ccctgaccga gggcgtccgc
     3121 gagatccagg ccgccgccac cgggcagcgg accggcccgg tgttcatggg catcgagggc
     3181 ggtgtgggca ccctcccgct cgccgtcgcc gagtccgtgc gctcccgcgg cggggagatc
     3241 cgcaccggcg ccccggtcac cgaactgcgc cgggagccgg ccggcggctg gcgggtcgtc
     3301 accggggagc gggagctgca cgccgacgcg gtcgtcgtcg ccgtgcccgc cccggccgcc
     3361 gcccggctgc tgcgcgccga ggcgcccggc gccgccgcgg agctggccgg cgtcgagtac
     3421 gcctccatgg cgctcatcac gctcgcctac cgccgcgcgg agaccgacct gcccgagggc
     3481 agcgggttcc tggtgccgcc ggtcgacggc cgcaccatca aggcgtccac cttcgcctcg
     3541 cgcaaatggg actggatcga cgaggagaac ccggacctca ccctcctgcg cacctccgtc
     3601 ggccggcacg gcgagacgga gatcctcggc cgcgacgacg ccggcctggt ggaggtctcc
     3661 cggcacgacc tgctggccgc caccggcctg gacgccaccc cggtcgccac ccgcgtcacc
     3721 cgctggcagg acggcctgcc ccagtacccc gtcggccacc acgcgcgcgt ggcccgcgtc
     3781 cgcgagcacc tcgccgccct cccggggctg gccgtgtgcg gcgcggccta cgacggcgtc
     3841 ggcatccccg cgtgcatcgc gagcgcgtac gacgccgccg acgccgtcgg ccagatccac
     3901 ggtgaggccc cgcacgacgt gcgggacgtc accgccaccc ggtgaagaac cctccacgga
     3961 ggagcaggag aataagggac atgaccgacg acgccaccac ccccgcaccc gaccggatcc
     4021 cgaacaaggg caagctggcc aaggacctga acgaggtcat ccgctacacc ctctggtccg
     4081 tcttcaagct gaaggacgtg ctgcccgagg accgcgccgg gtacgccgac gaggtccagg
     4141 agctgttcga ccagctcgcc gccaaggacg tcaccgtgcg cggcacgtac gacgtctccg
     4201 gcctgcgtgc cgacgccgac ctgatgatct ggtggcacgc cgagagcagc gaccagctcc
     4261 aggaggcgta caacctcttc cgccgcaccc ggctgggccg ggccctcgag ccggtctggt
     4321 cgaacatggc gctgcaccgg cccgccgagt tcaaccgctc gcacatcccg gcgttcctcg
     4381 ccgacgagga gccccgcgcg tacgtcagcg tgtacccctt cgtacgctcc tacgactggt
     4441 acctgctgcc cgacgaggac cgccgccgca tgctcgccga ccacggcaag atggcccgcg
     4501 gcttccccga cgtgcgcgcc aacacggtcc cctcgttctc cctcggagac tacgagtggg
     4561 tcctcgcctt cgaggccgac gagctgtacc gcatcgtcga cctcatgcgg cacctgcgcg
     4621 gctccgaggc gcggatgcac gtccgtgagg agatcccgtt ctacacgggc cgccgcaagg
     4681 acatcgcgga actcgtcgcg ggcctcgcct gagcgagcac cacggtgacc ggccccgccc
     4741 gcttctgaac ggggcggggc cggacccggt cacaccgccg gccgggcggg cgccgccttc
     4801 ccggccggca gacacgtctc cacgtggccg ttcgcgcagg tctcggaccc gcccgcggcc
     4861 ccgcgcgcac gcatgttccg ctcggccacc ggtgaccatg ccccgttcac gatccggacg
     4921 gagcgccgtc cgcacgtgat gtcccgaccg gccggtcgtg tgcggggaac gggtcccgtc
     4981 ccggctcgcg ggtcccggcc tcaacgccgc agcagcaccc gccgcgtggc gcgcgccagt
     5041 cgcgccgcgg gccggcgcga gtgcgggacc ggcccggacc gctcgagcca ccactcccgc
     5101 aactcccgca gcggcagctg ctcgtcccgc cgctcccgct gcccggaagt gagcagcaac
     5161 cgctcgacga agtccagggc gtcctgccgg taccccccgg tcatcggcca cctctgcgca
     5221 tgggcgagga aggccgcccg gtaccgctcg ccgagcagca ccggcagctc gggcgccacc
     5281 ttggcgacga cgtccgcccg cttcgcggcg agcgcccgcg cctggacggc catccgcgcc
     5341 cggtcgaacc cctcgggcac gggcgtcccg gcgaccagcg aggagagcac cgccgcctgg
     5401 gcgagcgcga cccgctgccg cgcgggtccg cgggagccgg actcccgcgc ggagccggaa
     5461 acgggaacgg agcgggaacg gacgccggag ccctgcgccc gtgccgtggt ccgcgcggcc
     5521 gctcccttcg ccaccgcctc ccgaatgccc gtcagttccc tctccagctc gtccggtgcg
     5581 gggaagtcct cgtcccgctc caggaggacg ccgggagggg agacccggga ggcgaggtcg
     5641 gtgaggatgt ccagtaccgg ccggggcacg ggatgggcgt ggctgtcgtg ccagacgccg
     5701 tcccgctcga cgccgcccgc gacgtggacg tacgcgatgg cctccagggg cagttcggcg
     5761 agcgccttcg ccgggtcctc gccgcggttg acgtggttgg tgtgcagatt ggccacgtcg
     5821 acgagcagcc gtacgccggt gcggtcggcc aggtcgtaca ggaactgccc ctcggacatc
     5881 tcctcgccgg gccaggagaa cagcgccgcg atgttctcca cggcgagcgg tacgggcagc
     5941 gcgtcctggg cgatccggac gttctcgcac agcacgtcga gcgcgtcccg ggtgcgcggc
     6001 accggcagca ggtgaccggc ctcgagccgg ggggaggcgc tgagcggtcc gcccgcccgc
     6061 acgaacgcga tgtgctcggt gaccagcggc gaccggagcg cctcggcccg ctcggcgagc
     6121 gcctccaggc gccccgcgtc cggccgttcg gccccgccga gcccgagcgc gaccccgtgc
     6181 ggcacgacgg tcaccccgcg ttcgcgcagc cgcagcagtg atgcggggac gtggccgggg
     6241 cacacgttct cggccaccac ctcgacccag tcgatccccg gcatccgttc gatgtccccg
     6301 gcgatctccg gccgccaccc gatccccgtc ccgagtcgct ccatgtcgct gtccccctcc
     6361 cggaccggcc gtcccgacgg cccccaccgc ctccggcggc cctcgtcggg cgtccgccgg
     6421 acgtgacggg ggtatggccc cgaagcccgc gtccgaaccg tgccgaggcc gccttcagag
     6481 gaacatttga gctttgggcc ggagtcgccg gacgggtccc gggaagcagg ggcggcggaa
     6541 acgccgttcc cgcgcgggac ggtcctggac aggccgtctc ctcggcgtgt ttccacggcg
     6601 gacctcccgc ggccctcttc gtcccgggtg ggccgggacg cgccggctcc gggacggtct
     6661 cacgtactcg ccggccccga cgatcccccg gaagtgtcgt cgacggggtc atcggccggc
     6721 cgaggcgact ccgcggaggc cagggcgtcc gccagttccg cgaccttgac cgtatccatg
     6781 tcctgcggaa tcagcacggt gaccgctccg cgcgtcagcg tcagcggtgg agcctgcggg
     6841 ggccgggtcg cgaaccatcg gcgcacactg tccaggaagg cgggcagggc cagggtcgtg
     6901 gagagcacca gggcaaggac ggacagaggg tcgcccaggt actcgtcgtg tgctggactc
     6961 cgccggggcg aggccgtgct caggcgcaga gagccgcgca gccggtcatc ctccaggagc
     7021 catcgttcga ggccggcgcg gatcgtgggg tcggccgggt cgacgtggaa ctcgaactcc
     7081 gtcatgcggc gctccgtctc ggttcagtcg gctgccggcg gtggaccggg gtgccagcgg
     7141 ttgggaatga gggggatcag gtggccgtcg ttgcgtacct ggcgatgcgg tccgggccat
     7201 ttcctgtccg cgcacacccg ccgtgccccc tcgaacagcg attccgcgtc caggagcggc
     7261 ggtccacccg gcagaccctg ctcgaggatg ccgaggagag cgtcggtgaa agcggtacgc
     7321 ggtcttcctc tcggtgccag ggagacctcg gtcgcgccgg aggaagccat gaggaacgtc
     7381 ccctcgatcg gagggaggag agggccggcg gcagcgagcg cgtcgagtcc ccgagcgctg
     7441 tagcagcagt cgatgatcac aaggcgctgg gaggccggag aggtggcgac cgcttcccgg
     7501 acctgttcga acggcaggaa ggtgtgcggg gaggagacgc gtgagtcggc cacggacagg
     7561 agcagttgac ctgtacgggg gtggggaacg ccatggccgg cgtagtacac caggagcatt
     7621 ccgctctcga aggcggtcgc ccggccggcc cgctcgatcg cgtccatgac cgcgcgcgga
     7681 tccagcgggt tgtccacgac gaggcaccgg tcccggggta tcccgaacga ggtcgtcagg
     7741 agcttgcgca gatcctccag gttccccttg acgccgggtt ggtcgctcag ctcctggtag
     7801 gtgtggaccc ccaccagaac gcacgcgccg gtgctccagt cggagggcct tcggatcggt
     7861 ggcggaggcg acgggtggcg ggcccgtgcg gtgtcaccgg tggcgcccgg gaacggcggc
     7921 cgggtccgcg gtctgccggg agcgtggagg accttgagca gttcacgccg ggcgctctcg
     7981 tggtcgagtc cgtacaggga cagcgccaga agcggcctga ggagcttcgg cggctcggtc
     8041 tcgtcgatgc gcaccgggat gatccggtcc ccggcggcga ggaccgcggc ccagtgatcc
     8101 gcggtggagt gacggggatc gaagtaggcc tctgagaaca gcgccagcac ctggccgttc
     8161 tccacggcgt ggcccagctc gatctgccgg ttgtctccgg cccgccagtc ccagcggtcc
     8221 agccggaccc ggtgtccggc ctcctgcaac tcccaggcca cccactcggc ccaggagcgg
     8281 tcgggctcgg cataactgat gaagagaaaa ggaggcttcg ccgtctgcga catcccgtct
     8341 cccaacctgc cccccgtcag ggctgtacgt gcggcggagt cacggcctcg ccgggctgcg
     8401 accggtccgc gagccgcccg ggaccactag cctggaggca tcatgcccgg gtccgagagc
     8461 ttgactcacg cgacggcttg tttcggaggg agcgatccat gggttctcac aagggacagg
     8521 aaggcctgag ggagcagtac aagttcggtg actcgacgac ggcggaaacg cggctggtga
     8581 cggtcgcccg ggtcttcgag ccgagcagca gggctttcct gcagcgtcac gccgcccccg
     8641 gggcgtcact cgcggtggac ctgggctgcg gtcccgggca ctccacacga ctgctgcacg
     8701 aagtcgtgcg gccggatcgc accctcggcc tggaccggtc cgcgagcttc ctcgcgacgg
     8761 cttcggcgag cgccctgccc ggcatgctct ttcgtcgcca cgacgtcacc gtcaccccgt
     8821 taccggtcga gcagcccgac atcctctacg cccggctgct tctgtcgcat ctgaaggagc
     8881 cggtccgtat ggtgcgtgcg tgggtcgccg agctgacgcc ggggggcagc ctcctcctcg
     8941 acgaggtcga atccgtcgaa acgagtgacg aaaccggcag ggactaccag aaagtcgtcc
     9001 agtcgatgct cctggacaac ggccagcgcc tcgacgtcgg ccgtctgctg accgctgcga
     9061 gccccgccgg agccggcagg gtccgacagg agctggtgac cgtgcggccc acctcgcgac
     9121 aggtgggtct gatgttcgcg ctcaacctgc gggtgtggcg ttcggacccg ttcctcgccg
     9181 cccggtactc ccccgactgg ctggacggcc tgcaggccga actggaccgt gccgcagaag
     9241 gcttgcggag cctcaccgtc acctggacca tgaggcaatt ggcgcttacg cgttgatccc
     9301 tcagcgcggt gtgcgacaag ggggactgcg atgggcggcg ccgtggacag gggagacttc
     9361 tacctcagct accaccctgc cgacctctcc tgggcacact ggctcagctg ggtgctcgag
     9421 cggaacggct acacggtctt cgtccaggac tgggacctca ggcccggtga gaacatcgtc
     9481 gcccgcaaag cccaggctct cgaccgctgc gagttcacac tggccgtggt gacgcgcgcc
     9541 tatctcgcca ctcctgcctg ccgggacgac tggacttccg ccttcctcca ggatcctgac
     9601 ggccgggagc ggctcgtctg tgtgcagacc gaagtgtgcg acctcccgcg gcagatgaga
     9661 gtccgcgggg tgatcgacct gcaggaccgc gcggaggaag aggcccggac cgtccttctc
     9721 gaccacctcg atcccggccg cagacgcccc acggcccgtc cgggctatcc ccgtgccgtc
     9781 gacggcgggg tggaaccccc caggttcccc gccgtggcgc cgcgcatctt ccacgtaccc
     9841 ccgcgcaacg atcacttcac cggacgcctc caccacctgc gcggacttca cgagcacctc
     9901 caggcggtga cggtcctgca ggccgcggtg cacgggttgg gcggggtggg caagagccag
     9961 atagccatcg agtacgcgca ccggtacgcg acgtcgtacg acatcgtctg gtgggtggcg
    10021 gcggagcagc ccgcggtgat gcaggcggac atctccgcac tcgccgccgc gctggacatc
    10081 ccgctgacag gtgacgagcg tcgtcagagg gaagccctct acgccgagct gcggcgacgc
    10141 ggccgctggc tgatcatcta cgacaacgcg gagcaccccg acgacgtgta tcccctgccg
    10201 gagggcggac gggtcctcat cacgtcacgg aatccgaact gggccaacgt cgcgacagcc
    10261 gtgtccctgg acgtcctgac ggacgaggaa gccagcggct tcctcgacaa acgcctcagc
    10321 accggtgaca cccgcgacta cggcagcccc gcggacatca aggagctgag gcaacggctg
    10381 ggcaatcttc ccctggccct ggagcaggcg gcagccttca tccaggcggc cggcatcaac
    10441 gtccgcgact acctggggct gctctccacc agtcccaggg acgcgctctc cacgagcccg
    10501 ccggtcggct atgcgcggac cgtcatcgat acgtggatgc tgacgctgga caagatcgag
    10561 aagacctgcc cggacgccta cggacttctc ctgttctgcg ccttcctcgc acccgaccag
    10621 gtcccgcgaa gggtgttgag ccgacccggg ggtcacctgc cgcgccggct gggcagggtg
    10681 gtgaccagcc cttcgcgtta cttcgcggcg gtgagcgcgc tgcggcggta ttccatggtc
    10741 aaagccactc ccgaggagat ctcgttgcac cggctggtgc aatgggtggt ccggcagaga
    10801 gccgtccgca ccggccccca cccctccgaa ctcgtatcgg acgtggtgcg gtggctgctg
    10861 agcgtgtacc ccgtcagtgc cggggagccg gagaacgcgg ccctggccgg cacactgctt
    10921 ccccacgtac tggcggttca tgaccacgtg cccgggcacc ggcaccacac tccccggcgg
    10981 gagctcgcgg ccctcctgga gcgcactgca gtcttcctgc acgccagcac ccgcctgaag
    11041 accgcggaga ccctgttcga ttcggcactc cggtttctcg gcggcaccaa gcccacggac
    11101 ccccagctcc tgcgcaccga actggcctac gggcgcttac tgcaggatgt cggacggacc
    11161 gatgaggcgc agcacctgtt cgagcgggtt ctcacccact cgaggaaacg gaacgccggc
    11221 cgggcgggcc tcctcgcccc cgctctgatc gacatgggac gcctgctgca ggaggagggc
    11281 cggctgcggg aggcccgtga gcgggtgacg gaagcactgt cactggctcc gaccgcccgt
    11341 ctgc
//
