LOCUS       NZ_CP023697            10510 bp    DNA     linear   CON 12-OCT-2024
DEFINITION  Streptomyces prasinus strain ATCC 13879 chromosome, complete genome.
ACCESSION   NZ_CP023697
VERSION     NZ_CP023697.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces prasinus
  ORGANISM  Streptomyces prasinus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7647592)
  AUTHORS   Lee,N. and Cho,B.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Biological Science, KAIST, 291 Daehak-ro,
            Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008704445.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:58:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,683
            CDSs (total)                      :: 6,591
            Genes (coding)                    :: 6,375
            CDSs (with protein)               :: 6,375
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 81 of 216
            Pseudo Genes (incomplete)         :: 177 of 216
            Pseudo Genes (internal stop)      :: 19 of 216
            Pseudo Genes (multiple problems)  :: 56 of 216
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:55:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2804135
            Orig. end                         :: 2814645
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023697.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Korean Collection for Type Cultures.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10510
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="11"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10510
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10510
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5510](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5510)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            785..1075
                     /db_xref="GeneID:95535439"
                     /locus_tag="CP972_RS12830"
                     /old_locus_tag="CP972_12865"
     CDS             785..1075
                     /codon_start=1
                     /db_xref="GeneID:95535439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512958.1"
                     /locus_tag="CP972_RS12830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12865"
                     /product="chaplin"
                     /protein_id="WP_055605939.1"
                     /transl_table=11
                     /translation="MSRIAKAACVALGTGAVALSGAGAALADAGAQGVASHSPGVLSGN
                     LVQAPVHVPVNVCGNTVNVVGALNPAFGNTCVNEDGQGHKNHQNHQGAYGN"
     misc_feature    1212..1227
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            1286..2260
                     /db_xref="GeneID:95535440"
                     /locus_tag="CP972_RS12835"
                     /old_locus_tag="CP972_12870"
     CDS             1286..2260
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (52-280). E-value:
                     1.2e-20. Score: 66.1. Matches aSDomain:
                     nrpspksdomains_CP972_RS12835_Abhydrolase_1.1"
                     /codon_start=1
                     /db_xref="GeneID:95535440"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826208.1"
                     /locus_tag="CP972_RS12835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12870"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_055605940.1"
                     /sec_met_domain="PF00561 (E-value: 4e-15, bitscore: 54.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.3e-11, bitscore:
                     42.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTALPTLQGSLNTQRTGSVPPPAEQVEERTLVFEGFAYQCRIVH
                     QDAPRTAPLLLLGGSSQNRHSWQGHEKWLAPLCTVITVDLPGYGSADFLPAHYGIDFLA
                     AAVQHMLVETGMPPVNLFGGCFGEVVGLRFAQLHPGSVRRIVFCGAAMRLPEIYADAVP
                     RLSHALERGEIEAAAGGLVELFMSNPAAGQVRRHAAVARLLHRQFVNQTPDELGKAVEH
                     NTRLMTHGCYEPLPVPEVPTLVFTGEHDTLCTPQMGRELAATIPAARFTTVREADHLVT
                     VERRQESSELIGRFCTDRPIDDLPYCTPVESPGAGLSCATAHR"
     aSDomain        1442..2125
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CP972_RS12835_Abhydrolase_1.1"
                     /evalue="1.20E-20"
                     /label="CP972_RS12835_Abhydrolase_1.1"
                     /locus_tag="CP972_RS12835"
                     /protein_end="280"
                     /protein_start="52"
                     /score="66.1"
                     /tool="antismash"
                     /translation="PLLLLGGSSQNRHSWQGHEKWLAPLCTVITVDLPGYGSADFLPAH
                     YGIDFLAAAVQHMLVETGMPPVNLFGGCFGEVVGLRFAQLHPGSVRRIVFCGAAMRLPE
                     IYADAVPRLSHALERGEIEAAAGGLVELFMSNPAAGQVRRHAAVARLLHRQFVNQTPDE
                     LGKAVEHNTRLMTHGCYEPLPVPEVPTLVFTGEHDTLCTPQMGRELAATIPAARFTTVR
                     EADHLV"
     gene            complement(2386..2664)
                     /db_xref="GeneID:95535441"
                     /locus_tag="CP972_RS12840"
                     /old_locus_tag="CP972_12875"
     CDS             complement(2386..2664)
                     /codon_start=1
                     /db_xref="GeneID:95535441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987900.1"
                     /locus_tag="CP972_RS12840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12875"
                     /product="hypothetical protein"
                     /protein_id="WP_055605941.1"
                     /transl_table=11
                     /translation="MVKSSKFAAVAGVLGGLALIGAGSVQAFGAEDPDNCVEDEKGTIR
                     CVQVSEQEITTDQYGKIHFVNNSKQTCSGESAEVSCTNSVVLPAEES"
     gene            complement(2810..3076)
                     /db_xref="GeneID:95535442"
                     /locus_tag="CP972_RS12845"
                     /old_locus_tag="CP972_12880"
     CDS             complement(2810..3076)
                     /codon_start=1
                     /db_xref="GeneID:95535442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527571.1"
                     /locus_tag="CP972_RS12845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12880"
                     /product="hypothetical protein"
                     /protein_id="WP_055605942.1"
                     /transl_table=11
                     /translation="MRKYQKAAVVMAMLGSAGFLGAGVGHANDGPEVELNNEQTTTCVQ
                     DNSKQGLINIDDINLAVGILGLGNAENTETNAVSCTSSLAIGG"
     misc_feature    3176..3191
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.99"
                     /tool="antismash"
     misc_feature    complement(3179..3195)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.96"
                     /tool="antismash"
     misc_feature    3180..3189
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.63"
                     /tool="antismash"
     misc_feature    complement(3181..3194)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.53"
                     /tool="antismash"
     misc_feature    3182..3195
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.61"
                     /tool="antismash"
     misc_feature    complement(3183..3196)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.42"
                     /tool="antismash"
     misc_feature    3183..3197
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.29"
                     /tool="antismash"
     misc_feature    complement(3184..3197)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            complement(3225..3503)
                     /db_xref="GeneID:95535443"
                     /locus_tag="CP972_RS12850"
                     /old_locus_tag="CP972_12885"
     CDS             complement(3225..3503)
                     /codon_start=1
                     /db_xref="GeneID:95535443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826207.1"
                     /locus_tag="CP972_RS12850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12885"
                     /product="hypothetical protein"
                     /protein_id="WP_055605943.1"
                     /transl_table=11
                     /translation="MLEPKRVAAAAGLLGGVVLIGAGAVQAAGVEGSGNCVGDGRGGIR
                     CEQVREYRLPADLQGKVRFTGRQEQTRSGSGAEVSCVNGTLLGGERA"
     gene            complement(3647..3916)
                     /db_xref="GeneID:95535444"
                     /locus_tag="CP972_RS12855"
                     /old_locus_tag="CP972_12890"
     CDS             complement(3647..3916)
                     /codon_start=1
                     /db_xref="GeneID:95535444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527571.1"
                     /locus_tag="CP972_RS12855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12890"
                     /product="hypothetical protein"
                     /protein_id="WP_055605944.1"
                     /transl_table=11
                     /translation="MRKYQKTAVVMAMLGSVAFLGAGVSHAGDDPSFKLNNEQTTACAQ
                     DNSVQGLVNIDDVNAGVGAILGLGNAENTETNAVACASSFTFGG"
     misc_feature    3970..3988
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.42"
                     /tool="antismash"
     misc_feature    4030..4043
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.43"
                     /tool="antismash"
     gene            complement(4151..5017)
                     /db_xref="GeneID:95535445"
                     /locus_tag="CP972_RS12860"
                     /old_locus_tag="CP972_12895"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /db_xref="GeneID:95535445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046795.1"
                     /locus_tag="CP972_RS12860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12895"
                     /product="tyrosinase family protein"
                     /protein_id="WP_055605945.1"
                     /transl_table=11
                     /translation="MVYVRKDAGTLTAAERRRFVEALLEVKRRGEYDEFVRMHLAYFSS
                     DGERGLRTAHMAPSFLPWHRRFLLDLETALRRVDPAVTLPYWDWTRDRTAASAPWTADF
                     LGGDGRRSDRQVMTGPFAYRQGDWTIEVGVTDGEFLTRDLGRRRDPLDLPTASDLEQAL
                     RDPVYDVAPWDSTVTRGFRNRLEGWGTGRGGAAWRNHNRVHRWVGGAMLGAASVNDPVF
                     WLHHAFVDLQWYRWQRRHGGARYLPARPLGPRDGQHRRVVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(5001..5510)
                     /db_xref="GeneID:95535446"
                     /locus_tag="CP972_RS12865"
                     /old_locus_tag="CP972_12900"
     CDS             complement(5001..5510)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95535446"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="CP972_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12900"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_055605946.1"
                     /sec_met_domain="melC (E-value: 4.8e-47, bitscore: 158.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVPMGPASGGAGEAAPRPAPPGPAGRTRRDVARGLRAGALAGVL
                     APVLTAFRSPRPGPAETRPPGDTAPAFERTYRGRRIRGVWTPGRATAEDGRWHVTVDGR
                     PLHLMRRADGGWLSAVDHYCSYRTPLEAARAAVDALGPGEQLRDAAPAPKDTGHAHGEG
                     RHGVRA"
     gene            complement(5646..5918)
                     /db_xref="GeneID:95535447"
                     /locus_tag="CP972_RS12870"
                     /old_locus_tag="CP972_12905"
     CDS             complement(5646..5918)
                     /codon_start=1
                     /db_xref="GeneID:95535447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987888.1"
                     /locus_tag="CP972_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12905"
                     /product="hypothetical protein"
                     /protein_id="WP_055605947.1"
                     /transl_table=11
                     /translation="MKSLKAAAVIAGSLIAAGMAAPAYAQEVDLASTGLDTGLNTRTAV
                     PFELMSPQDESGAGESRTSVFDTAGEAADAVNQAKPVRGDLGLHA"
     misc_feature    complement(6246..6260)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.41"
                     /tool="antismash"
     misc_feature    6411..6425
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.98"
                     /tool="antismash"
     misc_feature    6411..6426
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.01"
                     /tool="antismash"
     gene            6469..7566
                     /db_xref="GeneID:95535448"
                     /locus_tag="CP972_RS12875"
                     /old_locus_tag="CP972_12910"
     CDS             6469..7566
                     /codon_start=1
                     /db_xref="GeneID:95535448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="CP972_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12910"
                     /product="hypothetical protein"
                     /protein_id="WP_055605948.1"
                     /transl_table=11
                     /translation="MRQSLGQLLRPATVGVLALVAMWAPGGSPAAAPAAPPAPEASGLA
                     FDQRYYALQHGGIVRAANSSATCRTAGSSSCADVQAGAQAVNGDFDMFYVDVDRDPNTY
                     NSSRAEVRLPSGSQVTYARLYWGGNLRVGEQKPSEDNGRVLIAEPGGDYKELLADTVVG
                     HDVADGADAFQASADVTGLVRDSGPGPYTVAQVNVAAGRSEAGAWGGWTLVVAYENAAE
                     PLRHLSLWDGFAPLKSRTGPEVGLSGLDLAEGAEGQVGLVAYNGDRGTTGDSLTVTSGT
                     PASTTTLADSANPAGDVLNSTVSGPAGAAAARVPAYANTLGYDSDVFTLGRGLLRAGDR
                     MSLRLSSQRDAAWAGVLFTAVDARR"
     gene            7563..8906
                     /db_xref="GeneID:95535449"
                     /locus_tag="CP972_RS12880"
                     /old_locus_tag="CP972_12915"
     CDS             7563..8906
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /db_xref="GeneID:95535449"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527564.1"
                     /locus_tag="CP972_RS12880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12915"
                     /product="glycosyltransferase"
                     /protein_id="WP_079060819.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.5e-31,
                     bitscore: 107.9, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPSPVPDAAGEEPGRADPSPADRRPRVLHLTQPVDGGVARVVA
                     DLVRTQVADGLDVTAVCPDSTLALHLRALGAQVRDWRSTRSPGPSLVREVRHLARLIDE
                     VRPDLVHAHSAKAGLAGRLAVRGRIPTVFQPHAWSFEAVGGVTAALALGWERWGARWAT
                     RMVCVSEAERATGVRAGITGRWTVVPNGIDPERFRPAGPGTVRAGLAPLAALHPATPLV
                     VCVGRLCRQKGQDVLLRAWDTVVRRVPAARLVLVGDGPDDTLLRARAPESVLFAGAVAD
                     ASPWYQAADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGARESLPPAFAPRCLVPPE
                     DPEALAGALAGLLLDPPLRESLGGLGRRHVLSVHDVRHTAEQISGVYRDLLGTRPSGGR
                     ARPPRAVEPGGAHDTGQGQPFDAGCLAEASHRPTQRVEPTEYRETIRS"
     gene            8903..10345
                     /db_xref="GeneID:95535450"
                     /locus_tag="CP972_RS12885"
                     /old_locus_tag="CP972_12920"
     CDS             8903..10345
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95535450"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065681.1"
                     /locus_tag="CP972_RS12885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CP972_12920"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_055605949.1"
                     /sec_met_domain="Bac_transf (E-value: 4.5e-59, bitscore:
                     197.5, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAERTVTSPGVPPRDHGSSPVSVMPPRDTAGGPRLPDLRAPARP
                     ASPVPLFLADAAAALLGTLTLSGDQRYAPLVALLIGVSLLLRLRGDRSPEAGLSEAGPL
                     EELPSVCGRVAVAWLASAALWAAYRPEHALSAGAVLTGLVLQSAAGGALRAAVHGRRRA
                     ALRRRPHAALVVGPAATAQRVAAALLRHPGCGVRPVGIVAEQPDGDGGLPVLRTGEEVE
                     RAVIQNGVRAVLAVHPSVRAGQGPLLHTLAEAGCVVREVDADALPYPAEERLAGFPCRR
                     LDLSPARRDSAGKRVLDVLVSGTLLLLAGPLLLVCALTLRVSDGPGVVFRQERIGRGGK
                     PFTLLKFRTHRPVDAHESATRWSVADENEMRRFCRMLRRTSLDELLQLWNVLRGDMSLV
                     GPRPERPYFVSQFSRTYPGYAARHRMRAGITGLAQVHGLRGDTSIEDRCRFDNAYIDDW
                     SLWQDVRILLRTAVTLVRPTGS"
ORIGIN
        1 ggcgggacgg aggccggcct gccggacctc gaggacccgg acgtggagga cgaggtccgt
       61 ccgctgctgg ccgcgttcac ggagtacgcc gagcgcgtcg ggaccgttcc gttcgtgcgc
      121 cggacctgcg gactgcgcgg ggtggagctg aaccccccgt actaccgggc cgactacgga
      181 acggacttcc tgaagaaggg ggaggaggcc gtggccgtct gccggaacga gccggagcgg
      241 ttcgacgagc tgtacgccga ggccctgcgg gacgcctgcg agcaggaggg cgggccgtgg
      301 ctgaacctcc tggagttcgg cgaggacgcg atcttccaca ccggggacgg cgaggtgggc
      361 tggctcgtca accgcgacga cctgctggcg gggcgcttcg acgacgtcgt gcagtcgtac
      421 cactgctgat cccggcccag cggcccgccc tcccctccgg atcgtcgact cccagcccct
      481 cggccgggag tcgacgatcc cggccggcgg tccctccgcg acggccgggc cggaccgcat
      541 cgcgctgagg tggtcggcgc agcggcaggc ccgatgcgcc ggcgcaccgg tccgccggcg
      601 cccgccccga gcgcggccgg cagaccggac cggcagacct gatcggaagg ccggccggaa
      661 gaaaactccc tcacctcggg tgatccgcgg tcgatcaccg tgacccgtcg cgcctctccg
      721 cgttgaacgg gaagcacggc cgcaccccct gcgtccgcgc tcatcgaaca agaaggagaa
      781 cgtgatgtcg cgcatcgcga aggctgcctg cgtcgcactc ggcacgggtg ccgtggccct
      841 gagcggggcc ggtgcggctc tggcggacgc gggcgcccag ggtgtcgcct cccactcccc
      901 gggagtgctg tcgggcaacc tcgtccaggc cccggtccac gtgccggtca acgtgtgcgg
      961 gaacaccgtc aacgtcgtcg gcgcgctgaa cccggcgttc ggcaacacct gcgtgaacga
     1021 ggacggccag ggtcacaaga accaccagaa ccaccagggc gcgtacggga actgacccct
     1081 cgtaggcgtc accagcagga cgtccagagc cccggcacct cgagcgccgg ggctctcgcc
     1141 gttcccggca ccgccctcga tcctcccggc gcacggacac gccctaccgc caggatcgcc
     1201 cacaaatgag tgattccgga tgaaaaccgt gatggctggt aatgatggac cggttcacac
     1261 catttccccc gcaccggagc cgtccatgcg cactgccctc cccaccctcc agggttcact
     1321 gaacacgcaa cgcaccgggt ccgttcctcc acctgcggag caggtggagg aacggaccct
     1381 ggtgttcgag ggcttcgcgt accagtgccg gatcgtccac caggacgctc cgcgaacggc
     1441 gccgctgctg ctgctcggag gctcgtccca gaaccggcac tcctggcagg ggcacgagaa
     1501 gtggctggcg ccgttgtgca cggtgatcac cgtggatctc cccgggtacg gcagtgcgga
     1561 cttcctgccc gcccactacg ggatcgattt cctcgccgcc gcggtccagc acatgctggt
     1621 ggagaccggg atgccgccgg tgaatctctt cggcggctgc ttcggtgaag tcgtcggcct
     1681 ccgcttcgcg caactgcacc ccgggtccgt ccggcgcatc gtcttctgcg gcgccgcgat
     1741 gcgcctcccc gagatctacg ccgacgcggt accccgcttg tcgcacgcgc tggaacgggg
     1801 cgagatcgaa gcggcggccg gcggcctggt ggaactgttc atgtcgaacc cggccgcggg
     1861 ccaggtccgc cggcacgcgg ccgtcgcacg ccttctgcac cgccagttcg tgaaccagac
     1921 gccggacgag ctcgggaagg cggtcgaaca caacacgaga ctgatgacgc acggctgcta
     1981 cgagcccctg cccgtccccg aggtgccgac gctggtgttc acgggcgagc acgacaccct
     2041 gtgcaccccg cagatgggca gggagctcgc cgcgacgatt ccggcggcgc ggttcaccac
     2101 ggtcagggag gcggaccacc tggtcacggt cgagcgccgg caggagtcgt cggagctgat
     2161 cggccgtttc tgcaccgacc gccccatcga cgacctgccg tactgcactc ccgtggagtc
     2221 gccgggtgcc ggcctctcct gcgcgacggc ccaccggtga cggacggacg acgagtggcc
     2281 ggaacccggt accgggttcc ggccactctc cgccgtacgg atgatccgag gcgggatgcc
     2341 gacgcgaagg atgccgcgga caacggcact cggccgtcgg agacttcagg actcctcggc
     2401 cgggaggacg acgctgttgg tgcaggagac ctcggcgctc tcgccggagc aggtctgctt
     2461 cgagttgttg acgaagtgga tcttcccgta ctggtcggtc gtgatctcct gctcgctcac
     2521 ctgcacgcag cgaatggtgc ccttttcgtc ctcgacgcag ttgtccgggt cctccgcgcc
     2581 gaaggcctgg acggaaccgg cgccgatcag cgcgagccca ccgaggactc ccgccaccgc
     2641 tgcgaacttg ctcgacttca ccatctacct ttttccttga cgatcaacgc gccgcgagtg
     2701 agcggcggag ccggcccgga tctccgggcc gaccgatgtg ccgggcgggc ccggggctcg
     2761 aacgcacggc tcgagacccc gggcccaccc gcatcacctg tgagggtgat cagccgccga
     2821 tggcgaggct ggaggtgcag gaaaccgcgt tggtctcggt gttctcggcg ttgccgaggc
     2881 ccaggatgcc aaccgccagg ttgatgtcgt cgatgttgat cagaccctgc ttcgagttgt
     2941 cctgcacgca ggtcgtggtc tgctcgttgt tgagctcgac ctcgggcccg tcgttggcgt
     3001 ggccgacacc ggcgccgagg aatccggcgc tgccgagcat ggccatgacg acagcggcct
     3061 tctggtactt gcgcattact tctccttcta gtctgacacg gtccgtgatg gcccggtcac
     3121 tgacagtcgc taatgtaggc gacgattccg gcaattgata gacgaaacgc cgtatcgcgc
     3181 gtttttttca aacaagtcgg cattacaccc gagcggcgga ggcctcaggc cctctcgcca
     3241 ccgaggaggg tgccgttcac gcaggagacc tcggcacccg aaccggagcg ggtctgctcc
     3301 tgccggccgg tgaagcggac cttcccctgc aggtcggccg ggagtcggta ctccctcacc
     3361 tgctcgcagc ggatgccgcc cctgccgtcc ccgacgcagt tgccggagcc ctcgacaccg
     3421 gcggcctgga cggcaccggc gccgatcagg acgacgcctc ccaggagacc cgccgccgct
     3481 gcgaccctct tcggctcgag catctgcctt ttccctgacg gtcaccccgc cgcgtctcgg
     3541 cggcgggacc ggcccggaac tccgggccgg ccgacgcacc gggcggatcc ggagcccgaa
     3601 cgcgcggttc gagctccggg acccgcctgg atcacctgaa aggtgatcag ccgccgaagg
     3661 tgaagctgga ggcgcaggcc accgcgttgg tctcggtgtt ctcggcgttg ccgaggccca
     3721 ggatggcgcc gacgccggcg ttgacgtcgt cgatgttgac gaggccctgg accgagttgt
     3781 cctgggcgca ggccgtggtc tgctcgttgt tgagcttgaa cgacgggtcg tctccggcgt
     3841 ggctgacgcc ggcgccgagg aaggcaacgc tgccgagcat ggccatgacg acagcggtct
     3901 tctggtactt gcgcattgct tcttctccga tttcagaagt tgacacagcc cgtattgggc
     3961 cggtcaccga cagtgcataa cgtaagcgac gtctacggct ttggacagcc aaaacaccca
     4021 tgtgcgtaaa tttcttgcga aagtgcacgt cgcgggcctg tcgcacgcat gtcgactgct
     4081 ccgaaagcgt gtgtccgccc cggcacccgc gcgccggggc tctcctcgtg cagccggttc
     4141 cggtgccgcc tcaggcgtac cggtagatcc ggccgacgtc ctccagttcg tccggtgtca
     4201 cgtcccacgg cggcagcttc tcgtgccgcg ccacgacccg gcggtgctgc ccgtcgcggg
     4261 ggcccagcgg tctcgcgggg aggtagcggg ccccgccgtg ccggcgctgc cagcggtacc
     4321 actgcagatc gacgaaggcg tggtgcagcc agaagacggg gtcgttgacg gaggcggcgc
     4381 cgagcatggc cccgccgacc cagcggtgca cccggttgtg gttgcgccag gccgcgccgc
     4441 cccggccggt gccccagccc tccagccggt tgcggaagcc ccgcgtgacc gtcgagtccc
     4501 agggagccac gtcgtagacg gggtccctca gcgcctgctc caggtcgctc gcggtgggca
     4561 ggtcgagcgg gtcgcggcgg cggccgaggt cccgggtgag gaactccccg tccgtcacgc
     4621 ccacttcgat ggtccagtcg ccctgccggt acgcgaacgg ccccgtcatc acctgccggt
     4681 cggaacggcg cccgtcgccg ccgaggaagt ccgccgtcca cggcgcggag gcggccgtgc
     4741 ggtcccgggt ccagtcccag tagggaaggg tcaccgccgg gtccacccgg cgcagggcgg
     4801 tctccaggtc gagcaggaac cggcggtgcc agggcaggaa ggagggcgcc atgtgcgcgg
     4861 tgcgcagtcc gcgctcgccg tcggaggaga agtacgcgag gtgcatccgc acgaactcgt
     4921 cgtactcgcc ccgccgtttg acctccagca gcgcttcgac gaagcgtcgc ctctcggccg
     4981 ccgtgagcgt gccggcgtcc ttacgcacgt acaccatgcc ggccctcccc gtgcgcgtgt
     5041 cccgtgtcct tcggcgccgg tgccgcgtca cggagctgct cgccggggcc cagcgcgtcg
     5101 acggccgcgc gggcggcctc gagcggtgtc cggtaggagc agtagtggtc gaccgcgctc
     5161 agccagccgc cgtcggcgcg gcgcatcaga tgcagggggc ggccgtccac cgtgacgtgc
     5221 caccggccgt cctcggccgt ggcgcgcccc ggcgtccaca cccctcggat gcggcggccg
     5281 cggtaggtcc gctcgaaggc gggggcggtg tcgcccgggg gccgcgtttc cgcgggcccg
     5341 gggcgtggag accggaaggc ggtgagcacc ggagccagga cgccggcgag ggcgcccgcc
     5401 cgcaggcctc gcgccacgtc ccgccgtgtt ctgcccgccg ggccgggcgg cgccgggcgc
     5461 ggcgccgctt ccccggcgcc cccggatgcc ggtcccatgg gaacgaccat gatccactcc
     5521 ttgtccgaac ggttgtcgcc gttccaaccg tcggagccgc cgtatggtca cctccgcgcg
     5581 gcgaggggga cacacgaagc aggccccccg gtccggaccg gggggcctgc ttcgtacggc
     5641 gcgtctcagg cgtgcagccc cagatcgccg cgcaccggct tggcctggtt caccgcgtcc
     5701 gccgcctccc ccgcggtgtc gaacaccgac gtccgggact cgccggcgcc cgactcgtcc
     5761 tgcggcgaca tgagctcgaa ggggaccgcg gtgcgcgtgt tcagaccggt gtcgaggccg
     5821 gtcgacgcca ggtcgacctc ctgcgcgtac gcgggcgcgg ccataccggc ggcgatcagg
     5881 gacccggcaa tgacggcggc agccttcagg gacttcatcg tgttcctttc ttcggcaacg
     5941 cgggtcgccg gagggggctt tgtgtcgccg tgctcaacga tcctcggccc gtacggaaac
     6001 cgggggatca cggattgacg agtcgtcata tgagattaaa cgggcggcgg cgtcaccggc
     6061 gtgggcggaa cggcgaccgc gggcgccggg gttgccgatc cgcacgagcg gcgcggcaca
     6121 ccttcgacgc ccccttttca cccgtacggc cctgatccgt ccgacggggc gacaggactg
     6181 cgccacccgg gtgccacgtc gcgcgggccg gtttcacgtt tccaacacac ttgtcggcgt
     6241 tgattgagtt tcggcaaatt tgtccggccg gtggctagag ttgcgccgac gcacccccag
     6301 gagcggacgc atcgcactcc tcgtgctgcc tgctccgtcc cggcggtccc gctcgtcgcc
     6361 gtcctcgggc gaggctccgg caccggcggc accggcggca ccgccggccg cggacgtcgt
     6421 tttcacccat ccggtccccg ccacccgttc cgaaaggctc cgacggtcat gcgccaatcc
     6481 ctgggacagt tgcttcgccc tgccacggtg ggcgtcctcg ccctcgtcgc gatgtgggcg
     6541 cccggcggct cccccgccgc cgctccggcc gcgccgcccg ccccggaggc gagcggtctc
     6601 gcgttcgacc agcggtacta cgcgctccag cacggaggca tcgtgcgcgc ggccaactcc
     6661 tccgccacct gccgcacggc cgggtcgtcc tcgtgcgccg acgtgcaggc gggcgcgcag
     6721 gcggtgaacg gcgacttcga catgttctac gtcgacgtcg accgcgaccc gaacacctac
     6781 aactcctccc gcgcggaggt ccgtctgccc tccggctcgc aggtgacgta cgcgcgcctg
     6841 tactggggcg gcaacctgcg cgtgggcgaa cagaagccct cggaggacaa cgggcgggtc
     6901 ctgatcgccg aacccggcgg ggactacaag gagctcctcg cggacaccgt cgtgggacac
     6961 gacgtggcgg acggcgcgga cgccttccag gcctcggcgg acgtgaccgg gctggtgcgg
     7021 gacagcgggc cgggtccgta caccgtggcg caggtcaacg tggccgccgg ccgctcggaa
     7081 gccggcgcgt ggggcggctg gaccctggtg gtggcgtacg agaacgcggc ggagccgctg
     7141 cggcacctct cgctctggga cgggttcgcg ccgctgaagt cccgcacggg gcccgaggtc
     7201 gggctcagcg gtctggacct cgcggagggc gcggagggcc aggtggggct ggtggcgtac
     7261 aacggcgacc gcggcaccac gggcgactcg ctcaccgtga cctccggcac gcccgcctcc
     7321 accacgacgc tcgccgactc cgccaacccc gccggcgacg tcctgaactc caccgtcagc
     7381 gggcccgccg gggccgctgc ggcgcgggtg ccggcgtacg ccaacacgct cggctacgac
     7441 tccgacgtgt tcaccctcgg ccgcggtctg ctgcgcgccg gcgaccggat gagcctgcgg
     7501 ctctcgtccc agcgggacgc ggcctgggcc ggcgtgctct tcaccgccgt cgacgcccgg
     7561 cgatgagcac gccgtcaccc gtcccggacg ccgcgggtga ggaacccgga cgcgcggacc
     7621 cgtcccccgc cgaccgccgg ccgcgggtgc tgcacctcac ccagccggtg gacggcgggg
     7681 tcgcgcgcgt ggtggcggac ctggtccgta cgcaggtggc ggacggcctg gacgtcaccg
     7741 cggtctgccc cgacagcacg ctcgccctcc acctgcgagc gctcggcgca caggtgcggg
     7801 actggcggtc gacgcggtcg cccggcccgt cgctcgtgcg ggaggtgcgg cacctcgctc
     7861 ggctgatcga cgaggtgcgc cccgacctgg tgcacgcgca cagcgcgaag gccgggctcg
     7921 ccgggcggct cgccgtgcgc gggcggatcc cgaccgtctt ccagccgcac gcctggtcgt
     7981 tcgaggcggt gggcggggtc accgcggcgc tcgcactcgg gtgggaacgc tggggggcgc
     8041 gctgggccac acggatggtg tgcgtgagcg aggcggaacg ggcgaccggt gtgcgcgccg
     8101 ggatcaccgg acggtggacc gtcgtcccca acggcatcga ccccgagcgc ttccgcccgg
     8161 ccggccccgg caccgtgcgg gccgggctcg ccccgctcgc cgccctccac cccgcgacgc
     8221 cgctcgtggt ctgcgtgggg cggctctgcc ggcagaaggg gcaggacgtc ctgctgcggg
     8281 cgtgggacac cgtcgtgcgg cgggtgcccg ccgcacgcct cgtcctggtc ggcgacgggc
     8341 ccgacgacac cctgctgcgc gcgcgggccc ccgagtccgt gctgttcgcg ggagccgtcg
     8401 ccgacgcctc gccctggtac caggccgccg atctcgtcgt cctgccgtcc cgctgggagg
     8461 gcatggccct cgccccgctg gaggcgatgg cctgcggacg tccggtggtg gtgacggacg
     8521 tcgacggcgc ccgcgagagc ctgccgccgg ccttcgcccc ccgctgcctg gtcccgccgg
     8581 aggacccgga ggccctggcc ggggccctgg ccgggctgct cctcgacccg ccgctgcgtg
     8641 agtcgctcgg cggcctgggc cgtcggcacg tgctgtccgt ccacgacgta cggcacaccg
     8701 ccgagcagat ctccggcgtc taccgcgacc tgctcggcac ccggccctcc ggggggaggg
     8761 cacgtccgcc ccgggccgtc gagcctggcg gggcccatga caccgggcag gggcagccgt
     8821 tcgacgcggg ctgcctcgcc gaagcgtcgc accggcccac gcagcgcgtc gaacccaccg
     8881 agtacaggga gaccatccgc tcgtgaccgc cgaacgcacc gtgacctccc ccggcgtacc
     8941 gccgcgggac cacggatcct cacccgtctc cgtcatgccg ccgcgcgaca ccgccggcgg
     9001 cccgcggctg cccgaccttc gcgctccggc acggccggcc tcgcccgtac cgctgttcct
     9061 cgcggacgcc gccgcggccc tgctgggcac gctgacgctg tccggcgacc agcggtacgc
     9121 cccgctcgtc gccctgctga tcggcgtgtc gctgctgctg cgcctccgcg gcgaccggtc
     9181 gccggaggcg gggttgtcgg aggcggggcc gctggaggaa ctgccctccg tgtgcggccg
     9241 ggtcgcggtg gcctggctgg cgtccgcggc gctctgggcg gcgtaccggc cggagcacgc
     9301 gttgtccgcc ggcgccgtgc tgacgggcct cgtcctgcag tcggcggccg gcggcgcgct
     9361 gcgcgcggcc gtgcacgggc ggcggcgcgc cgcgctgcgg cgccgtccgc acgcggcgct
     9421 cgtcgtcggc ccggcggcga ccgcgcagcg tgtggccgcc gccctgctgc gtcacccggg
     9481 gtgcggggtg cggccggtgg ggatcgtcgc cgagcagccg gacggcgacg ggggcctgcc
     9541 ggtgctgcgc accggtgagg aggtcgagcg ggcggtcatc cagaacgggg tgcgggccgt
     9601 gctggccgtc cacccctcgg tacgtgccgg gcagggcccg ctgctgcaca cgctggccga
     9661 ggcgggctgc gtggtccggg aggtcgacgc cgacgccctg ccgtacccgg cggaggaacg
     9721 gctggccggt ttcccgtgcc gtcgcctgga cctgtcaccg gcgcgccgcg acagtgcggg
     9781 caagcgcgtg ctcgacgtcc tcgtgtcggg gacgctgctg ctgctggccg gcccgctgct
     9841 gctggtgtgc gcgctgacgc tgcgggtcag cgacgggccg ggcgtggtgt tccggcagga
     9901 gcgcatcggc aggggcggga agccgttcac gctgctgaag ttccgtacgc accggccggt
     9961 cgacgcgcac gagtcggcga cccggtggag cgtggccgac gagaacgaga tgcgccggtt
    10021 ctgccgcatg ctgcggcgca cctcgctcga cgaactgctc cagctctgga acgtgctgcg
    10081 cggcgacatg agcctggtcg ggccgcggcc cgaacgccct tacttcgtct cccagttcag
    10141 ccggacctat ccgggttacg cggcccggca ccggatgcgg gccggcatca ccggtctcgc
    10201 ccaggtccac ggcctgcgcg gcgacacctc gatcgaggac cgctgccggt tcgacaacgc
    10261 ctacatcgac gactggtcgc tgtggcagga cgtccgcatc ctgctgcgca ccgcggtcac
    10321 gctggtccgt ccgacgggga gctgaccgac gtgagcctcg cccccgcacc gctcccgtcc
    10381 ccgctcgccc gccgggccgc ccgcctggtg ccggtgctgc ccctggtggc gctgcccacc
    10441 ctgctggccc tgccggtgac gcacggcgga gcggggcccg cggacgtggt ctccgggctg
    10501 gtggtggtgt
//
